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2013

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Articles 451 - 480 of 1171

Full-Text Articles in Animal Sciences

Review Of The Subgenus Pandinurus Fet, 1997 With Descriptions Of Three New Species (Scorpiones, Scorpionidae, Pandinus)., František Kovařík Mar 2013

Review Of The Subgenus Pandinurus Fet, 1997 With Descriptions Of Three New Species (Scorpiones, Scorpionidae, Pandinus)., František Kovařík

Euscorpius

Pandinus (Pandinurus) awashensis sp. n. from Ethiopia, P. (P.) somalilandus sp. n. from Somaliland, and P. (P.) lowei sp. n. from Democratic Republic of Congo are described and compared with other species of the subgenus. P. (P.) sudanicus Hirst, 1911 stat. n. previously considered a synonym of P. (P.) magrettii Borelli, 1901 is recognized as a valid species. New data on taxonomic characters and distribution of the subgenus Pandinurus are presented. Also presented are a map of distribution, photos of the localities and a key to species using hitherto unpublished characters of the tarsomere spination formula and sexual …


Fish Assemblages Associated With Natural And Anthropogenically-Modified Habitats In A Marine Embayment: Comparison Of Baited Videos And Opera-House Traps, Corey B. Wakefield, Paul Lewis, Teresa B. Coutts, David V. Fairclough, Timothy J. Langlois Mar 2013

Fish Assemblages Associated With Natural And Anthropogenically-Modified Habitats In A Marine Embayment: Comparison Of Baited Videos And Opera-House Traps, Corey B. Wakefield, Paul Lewis, Teresa B. Coutts, David V. Fairclough, Timothy J. Langlois

Fisheries Research Articles

Marine embayments and estuaries play an important role in the ecology and life history of many fish species. Cockburn Sound is one of a relative paucity of marine embayments on the west coast of Australia. Its sheltered waters and close proximity to a capital city have resulted in anthropogenic intrusion and extensive seascape modification. This study aimed to compare the sampling efficiencies of baited videos and fish traps in determining the relative abundance and diversity of temperate demersal fish species associated with naturally occurring (seagrass, limestone outcrops and soft sediment) and modified (rockwall and dredge channel) habitats in Cockburn Sound. …


Geochemical Evidence Of Ancient Maya Marketplace Activities In The Puuc Hills Of Mexico And At Caracol, Belize, Jacob M. Horlacher Mar 2013

Geochemical Evidence Of Ancient Maya Marketplace Activities In The Puuc Hills Of Mexico And At Caracol, Belize, Jacob M. Horlacher

Theses and Dissertations

The large public plazas of the ancient Maya were likely swept clear of debris and durable artifacts that could have provided evidence of the ancient anthropogenic activities. However, geochemical residues of food or mineral ores and pigments became affixed to soil and floor particles. These particles chemically bound so that natural movement of water is insufficient to cause them to move, leaving invisible geochemical signatures of ancient activities. This line of study is focused on the relationship between the geospatial distribution of element concentrations and ancient human activities using current laboratory techniques and isopleths, or chemical concentration contour maps, to …


Habitat Selection And Response To Disturbance By Pygmy Rabbits In Utah, Robert John Edgel Mar 2013

Habitat Selection And Response To Disturbance By Pygmy Rabbits In Utah, Robert John Edgel

Theses and Dissertations

The pygmy rabbit (Brachylagus idahoensis) is a sagebrush (Artemisia sp.) obligate that depends on sagebrush habitats for food and cover throughout its life cycle. Invasive species, frequent fires, overgrazing, conversion of land to agriculture, energy development, and many other factors have contributed to recent declines in both quantity and quality of sagebrush-steppe habitats required by pygmy rabbits. Because of the many threats to these habitats and the believed decline of pygmy rabbit populations, there is a need to further understand habitat requirements for this species and how they respond to disturbance. This study evaluated habitat selection by pygmy rabbits in …


Effect Of Anthraquinone Dyes On The Carbohydrate, Protein And Lipid Content In The Muscle Of Channa Punctatus And Cyprinus Carpio, Rajee Olaganathan, Jamila Patterson Mar 2013

Effect Of Anthraquinone Dyes On The Carbohydrate, Protein And Lipid Content In The Muscle Of Channa Punctatus And Cyprinus Carpio, Rajee Olaganathan, Jamila Patterson

Publications

Channa punctatus and Cyprinus carpio was exposed to sub lethal concentrations viz., 6.15 and 6.60 mg/l of vat blue 4 and vat green 1 respectively for a period of thirty days. The cumulative effect of these two dyes on the total carbohydrate, protein and lipid levels in the muscle of both the fishes were assayed on 7th, 18th and 30th day of exposure. The mean carbohydrate content of C. punctatus (both control and treated fish) collected on 18th and 30th day showed a significant difference (P < 0.01 to 0.04). In the case of control and treated C. carpio the data collected on 30th day showed significant variation (P < 0.04) in the carbohydrate content of the muscle. Student’s ‘t’ test analysis conducted on the protein content of C. punctatus during different occasions registered a significant decrease; in case of lipid content, except between the 1st and 30th day in all other cases the ‘t’ value is not significant. In case of C. carp protein and lipid content were observed to increase during the 7th day and thereafter a marked decrease was recorded on the 18th and 30th day of exposure. When the data was subjected to student’s ‘t’ test, both in protein and lipid, significant difference could not be recorded.


Evaluating The Effects Of Road Crossing Structures On Stream-Associated Amphibians In The Wilson River Watershed, Tillamook State Forest, Oregon, Sara Erin Twitchell Mar 2013

Evaluating The Effects Of Road Crossing Structures On Stream-Associated Amphibians In The Wilson River Watershed, Tillamook State Forest, Oregon, Sara Erin Twitchell

Dissertations and Theses

As replacement and removal of undersized culverts gains momentum as an effective technique for restoring natural stream flows and removing fish passage barriers, it is important to evaluate the benefits of these efforts on the in-stream and adjacent riparian habitat for other species of potential concern. This study compares stream-associated amphibian (SAA) occurrence in streams adjacent to different road crossing structures on unpaved forest roads in the Wilson River watershed located within the Tillamook State Forest, Oregon. Surveys were conducted at road crossing structures for three taxa of SAA; Pacific giant salamander (Dicamptodon tenebrosus), coastal tailed frog (Ascaphus truei), and …


Microbial Responses To Coarse Woody Debris In Juniperus And Pinus Woodlands, Deborah Monique Rigby Mar 2013

Microbial Responses To Coarse Woody Debris In Juniperus And Pinus Woodlands, Deborah Monique Rigby

Theses and Dissertations

The ecological significance of coarse woody debris (CWD) is usually highlighted in forests where CWD constitutes much of an ecosystem's carbon (C) source and stores. However, a unique addition of CWD is occurring in semi-deserts for which there is no ecological analog. To stem catastrophic wildfires and create firebreaks, whole Juniperus osteosperma (Torr.) and Pinus edulis (Engelm.) trees are being mechanically shredded into CWD fragments and deposited on soils previously exposed to decades of tree-induced changes that encourage "tree islands of fertility." To investigate consequences of CWD on C and nitrogen (N) cycling, we evaluated microbial metabolic activity and N …


Connecting The Contradictory With Science Art And The Aid Of A Caption, Carel P. Brest Van Kempen, Darryl Wheye Mar 2013

Connecting The Contradictory With Science Art And The Aid Of A Caption, Carel P. Brest Van Kempen, Darryl Wheye

The Transdisciplinary STEAM+ Journal

When the disciplines of science and art intertwine to reveal a truth then words and images are suited to telling different parts, and reveal the whole story most effectively when working in tandem. Decoding the underlying science within a work of art through a caption does not diminish its value as art, but when we fail to decode the science we miss entry into a narrative.


A Distributed Intelligence Approach To Multidisciplinarity: Encouraging Divergent Thinking In Complex Science Issues In Society., Jarod Kawasaki, Dai Toyofuku Mar 2013

A Distributed Intelligence Approach To Multidisciplinarity: Encouraging Divergent Thinking In Complex Science Issues In Society., Jarod Kawasaki, Dai Toyofuku

The Transdisciplinary STEAM+ Journal

The scientific issues that face society today are increasingly complex, open-ended and tentative (Sadler, 2004). Finding solutions to these issues, not only requires an understanding of the science, but also, concurrently dealing with political, social, and economic dimensions that exist (Hodson, 2003). For example, 40 years after the first congressional hearing on climate change held by Al Gore in 1976, the 2012 Intergovernmental Panel on Climate Change (IPCC) report states that climate change is still getting worse, despite efforts by governments, businesses, social actors such as Non-Government Organizations, and scientists. With the top minds in the world, across all disciplines, …


The Influence Of Anthropogenic Development Of Water On Coyotes And Kit Foxes In The Great Basin And Mojave Deserts, Lucas Keith Hall Mar 2013

The Influence Of Anthropogenic Development Of Water On Coyotes And Kit Foxes In The Great Basin And Mojave Deserts, Lucas Keith Hall

Theses and Dissertations

Anthropogenic provisioning of water (water developments) to enhance abundance and distribution of wildlife is a common management practice in arid regions where water is limiting. Despite the long-term and widespread use of water developments, little is known about how they influence distribution, competition dynamics, and behavior of native species. To elucidate the potential influences of water developments on native species, we tested hypotheses concerning the occurrence and behavior of native kit foxes (Vulpes macrotis). First, we tested the indirect effect of water hypothesis (IEWH) which proposes that water developments negatively affect the arid-adapted kit fox by enabling a …


Application Of Genome Reduction, Next Generation Sequencing, And Kaspar Genotyping In Development, Characterization, And Linkage Mapping Of Single Nucleotide Polymorphisms In The Grain Amaranths And Quinoa, Scott Matthew Smith Mar 2013

Application Of Genome Reduction, Next Generation Sequencing, And Kaspar Genotyping In Development, Characterization, And Linkage Mapping Of Single Nucleotide Polymorphisms In The Grain Amaranths And Quinoa, Scott Matthew Smith

Theses and Dissertations

The grain amaranths (Amaranthus sp.) and quinoa (Chenopodium quinoa Willd.) are important seed crops in South America. These crops have gained international attention in recent years for their nutritional quality and tolerance to abiotic stress. We report the identification and development of functional single nucleotide polymorphism (SNP) assays for both amaranth and quinoa. SNPs were identified using a genome reduction protocol and next generation sequencing. SNP assays are based on KASPar genotyping chemistry and were detected using the Fluidigm dynamic array platform. A diversity screen consisting of 41 amaranth accessions showed that the minor allele frequency (MAF) of the amaranth …


Experimental And Natural Warming Elevates Mercury Concentrations In Estuarine Fish, Jennifer A. Dijkstra, Kate L. Buckman, Darren Ward, David W. Evans, Michele Dionne, Celia Y. Chen Mar 2013

Experimental And Natural Warming Elevates Mercury Concentrations In Estuarine Fish, Jennifer A. Dijkstra, Kate L. Buckman, Darren Ward, David W. Evans, Michele Dionne, Celia Y. Chen

Dartmouth Scholarship

Marine food webs are the most important link between the global contaminant, methylmercury (MeHg), and human exposure through consumption of seafood. Warming temperatures may increase human exposure to MeHg, a potent neurotoxin, by increasing MeHg production as well as bioaccumulation and trophic transfer through marine food webs. Studies of the effects of temperature on MeHg bioaccumulation are rare and no study has specifically related temperature to MeHg fate by linking laboratory experiments with natural field manipulations in coastal ecosystems. We performed laboratory and field experiments on MeHg accumulation under varying temperature regimes using the killifish, Fundulus heteroclitus. Temperature treatments …


Assessment Of A Rotenone Application Event At Mormon Island West Lake In Central Nebraska, Keith D. Koupal, Brian C. Peterson, Casey W. Schoenebeck Mar 2013

Assessment Of A Rotenone Application Event At Mormon Island West Lake In Central Nebraska, Keith D. Koupal, Brian C. Peterson, Casey W. Schoenebeck

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Fisheries managers applied rotenone to Mormon Island West in August of 2010 to renovate a fish community that was hypothesized to be unbalanced (i.e., dominated with gizzard shad and common carp) based on standardized survey results. We estimated species-specific biomass following the lake renovation to provide a baseline biomass estimate for a sand pit lake and to evaluate the effectiveness of standardized sampling gears. Gizzard shad (Dorosoma cepedianum) were abundant in all sampling gears, but mostly stock-size (>175 mm total length) and larger individuals were caught in gill and trap nets and sub-stock (≤175 mm total length) …


An Experimental Investigation Of Nest Reuse And Nest Site Selection In An Open-Cup Nesting Passerine, Sarah A. Cancellieri Mar 2013

An Experimental Investigation Of Nest Reuse And Nest Site Selection In An Open-Cup Nesting Passerine, Sarah A. Cancellieri

Dissertations and Theses

Eastern Kingbirds (Tyrannus tyrannus) breed from coast to coast in North America and build open-cup nests in trees. They have been extensively studied across most of their range and have only on occasion been documented to reuse a nest from a previous season. However, at Malheur National Wildlife Refuge (MNWR), located in southeastern Oregon, ~10 % of female Eastern Kingbirds reuse old nests of mainly American Robins (Turdus migratorius). In an attempt to address why nest reuse is so common at MNWR, I used artificial nests to evaluate two hypotheses as to why nest reuse is common in this breeding …


Status Of Nutria (Myocastor Coypus) Populations In The Pacific Northwest And Development Of Associated Control And Management Strategies, With An Emphasis On Metropolitan Habitats, Trevor Robert Sheffels Mar 2013

Status Of Nutria (Myocastor Coypus) Populations In The Pacific Northwest And Development Of Associated Control And Management Strategies, With An Emphasis On Metropolitan Habitats, Trevor Robert Sheffels

Dissertations and Theses

The nutria (Myocastor coypus) is a semi-aquatic rodent native to South America that was introduced to the Pacific Northwest, USA, in the 1930s. Primary damage categories from this invasive species include burrowing and herbivory, resulting in habitat degradation. Nutria have become well-established in metropolitan habitats, and anecdotal information suggests the problem has increased in recent years. However, little regional research on the species has been conducted. The scope of this research, which emphasizes metropolitan habitats, includes three primary foci in relation to nutria populations in the Pacific Northwest: modeling habitat suitability, assessing activity and movement patterns, and identifying and managing …


Eurasian Jays Predict The Food Preferences Of Their Mates, Alan C. Kamil Mar 2013

Eurasian Jays Predict The Food Preferences Of Their Mates, Alan C. Kamil

Avian Cognition Papers

The cognitive abilities of animals continue to fascinate both scientists and nonscientists. Although the abilities of the primates, our closest living relatives, generally attract most interest, several different lines of research have demonstrated high levels of intellectual capacity in birds, particularly corvids. The members of this family are known for their large brains and have performed well in many cognitive tasks using different paradigms (1–3). This finding has led to substantial revision of thinking about avian intelligence, including the suggestion of convergence in the evolution of cognitive abilities between corvids and primates (4). In PNAS, Ostojić et al. (5) add …


Ecological Studies Of Wolves On Isle Royale, 2012-2013, John A. Vucetich, Rolf O. Peterson Mar 2013

Ecological Studies Of Wolves On Isle Royale, 2012-2013, John A. Vucetich, Rolf O. Peterson

Ecological Studies of Wolves on Isle Royale

Annual Report 2012-2013


Tracking The Forest People: In The Rainforests Of Borneo, Erin Vogel '95 Studies A Group Of Orangutans In Hopes Of Protecting This Dwindling Species, Adriana Nordin Manan Mar 2013

Tracking The Forest People: In The Rainforests Of Borneo, Erin Vogel '95 Studies A Group Of Orangutans In Hopes Of Protecting This Dwindling Species, Adriana Nordin Manan

Colby Magazine

Erin Vogel ’95 studies orangutans up close in the rainforest of Borneo. An anthropologist at UC Santa Cruz, Vogel wants to know what these orangutans eat and why, in hopes that the knowledge will help protect this dwindling species.


The Search For The Ivory-Billed Woodpecker: Sara Barker And A Team Of Researchers Find Conclusive Evidence That Brings The Ivory-Billed Woodpecker Back From 'Extinction', Gerry Boyle Mar 2013

The Search For The Ivory-Billed Woodpecker: Sara Barker And A Team Of Researchers Find Conclusive Evidence That Brings The Ivory-Billed Woodpecker Back From 'Extinction', Gerry Boyle

Colby Magazine

An extinct ivory-billed woodpecker in an Arkansas swamp? Sara Barker '94 coordinated the team that quietly searched the swamp to see if the "grail bird" lives.


Linking Animal Behavior To Useful Natural Repellents, Sandra Avant Mar 2013

Linking Animal Behavior To Useful Natural Repellents, Sandra Avant

Agricultural Research Magazine

A little monkey business is revealing a few clues about natural remedies that animals use to protect themselves against biting insects and arthropods.

Certain species of animals, such as monkeys and birds, anoint themselves with citrus, other plants, and creatures like millipedes. To find out more about this behavior and to determine if any chemicals in the anointing substances effectively deter ticks and mosquitoes, scientists are examining responses to natural compounds.

Scientists at the Agricultural Research Service Henry A. Wallace Beltsville [Maryland] Agricultural Research Center (BARC) and the Smithsonian Conservation Biology Institute (SCBI) at the National Zoological Park in Front …


An Atlas For Guatemala, A Tool For Conserving World Crops, Karen Williams, Dennis O’Brien Mar 2013

An Atlas For Guatemala, A Tool For Conserving World Crops, Karen Williams, Dennis O’Brien

Agricultural Research Magazine

With exotic names like ayote de caballo (a wild squash), friajolito (a wild bean), and teocinte (a wild relative of corn), Guatemala’s native plants seem very different from the agricultural bounty produced by farmers in the United States and other countries. But many of these native plants carry genes that may be vital to global food security. A new tool, developed by a team that includes Agricultural Research Service scientists, will make it easier to find and preserve these important plants. The tool is an interactive atlas designed to provide Guatemalan scientists and land managers with information on where these …


Wasted Food: What We Are Doing To Prevent Costly Losses, Robert L. Fireovid Mar 2013

Wasted Food: What We Are Doing To Prevent Costly Losses, Robert L. Fireovid

Agricultural Research Magazine

Like many other public and private organizations, the Agricultural Research Service is very concerned about how much food goes to waste between farm and fork—both nationally and internationally.

By reducing losses in our food systems, U.S. growers, processors, and others can enhance America’s ability to feed itself and the world. By the same token, slashing waste may provide new opportunities to reduce costs along the entire supply chain and make better use of increasingly limited natural resources.

The U.S. Department of Agriculture’s Economic Research Service (ERS) estimated— in 2008—that the amount of food lost annually at just the retail and …


Measuring And Managing Impacts Of Manure Spills, Ann Perry Mar 2013

Measuring And Managing Impacts Of Manure Spills, Ann Perry

Agricultural Research Magazine

Manure spills happen for a range of reasons—a manure spreader rolls over, a hose breaks, a storage pond overflows after a relentless downpour. Whatever the cause, these events are such a threat to the environment that states have emergency teams to deal with the hazard.

Typically, the responders build dams to contain the spill and then pump out the contaminated water. Although cleanup efforts start as quickly as possible, a fish kill in a nearby stream is often the first evidence that a spill has taken place.

Another problem is that sediments in the contaminated water channel can capture phosphorus …


March 2013 Locations Featured In This Magazine Issue Mar 2013

March 2013 Locations Featured In This Magazine Issue

Agricultural Research Magazine

The Agricultural Research Service has about 100 labs all over the country.

Locations Featured in This Magazine Issue

Davis, California 3 research units ■ 117 employees Corvallis, Oregon 3 research units ■ 127 employees

Vegetable and Forage Crops Research Unit, Prosser, Washington 1 research unit ■ 35 employees

Northwest Irrigation and Soils Research Laboratory, Kimberly, Idaho 1 research unit ■ 34 employees

U.S. Arid-Land Agricultural Research Center, Maricopa, Arizona 3 research units ■ 80 employees

Northern Plains Agricultural Research Laboratory, Sidney, Montana 2 research units ■ 59 employees

Children’s Nutrition Research Center, Houston, Texas 1 research unit ■ 8 employees …


Trickery And Other Methods Explored To Vanquish Potato Cyst Nematodes, Roy Navarre, Jan Suszkiw Mar 2013

Trickery And Other Methods Explored To Vanquish Potato Cyst Nematodes, Roy Navarre, Jan Suszkiw

Agricultural Research Magazine

The pale cyst nematode, Globodera pallida, is one bad roundworm.

Unchecked, it invades the roots of potato and other host crops to feed, obstructing the free flow of nutrients and causing stunted growth, wilted leaves, and other symptoms that can eventually kill the plant. Severe infestations in potato fields can cause yield losses of up to 80 percent.

To make matters worse, female G. pallida nematodes form hard, round cysts that safeguard their eggs from predators and parasites, inhospitable conditions, or a scarcity of food. As many as 30 years may pass before the eggs hatch (cued by a …


How Does A Mom's Nutrition Affect Her Children's Health?, Robert A. Waterland, Marcia Wood Mar 2013

How Does A Mom's Nutrition Affect Her Children's Health?, Robert A. Waterland, Marcia Wood

Agricultural Research Magazine

In some rural villages of the tiny West African nation of The Gambia, food is generally less available during August and September—the peak of the rainy season—than during a typically dry March through May. Now, a study led by molecular geneticist Robert A. Waterland of the USDA-ARS Children’s Nutrition Research Center in Houston, Texas, has shown that functioning of certain genes in kids conceived during the rainy season differs from that in children conceived during the dry season.

The difference may be explained by a relatively new science referred to as “epigenetics.” The variation appears to be permanent, and in …


A Desert Shrub’S Crystallized Protein Sheds Light On Photosynthesis, Michael Salvucci, Dennis O’Brien Mar 2013

A Desert Shrub’S Crystallized Protein Sheds Light On Photosynthesis, Michael Salvucci, Dennis O’Brien

Agricultural Research Magazine

Plants use an enzyme known as “rubisco” to capture carbon dioxide from the atmosphere and, with energy from the sun and nutrients from the soil, build up the shoots, leaves, and stems that make up the plant itself. Scientists have known that for years. They also have known that temperatures are important. When it gets too hot, a rubisco helper protein called “rubisco activase” shuts down, photosynthesis stops, and the plant stops growing. Heat literally unravels the activase protein, and when it does, the result is a less bountiful harvest. Different plants shut down photosynthesis at different temperatures, and the …


Agricultural Research Magazine, March 2013 Mar 2013

Agricultural Research Magazine, March 2013

Agricultural Research Magazine

Table of Contents

4 Weight Loss, Cortisol, and Your Brain: Scientists Explore Connections

6 How Does Mom’s Nutrition Affect Her Children’s Health? Epigenetics May Provide New Insights

8 Enhancing Yogurt With Healthful Fiber From Oats

9 An Atlas for Guatemala, a Tool for Conserving World Crops

10 Linking Animal Behavior to Useful Natural Repellents

12 Cultural Practices To Maintain Soil Quality and Address Climate Change

15 A Desert Shrub’s Crystallized Protein Sheds Light on Photosynthesis

16 Measuring and Managing Impacts of Manure Spills

18 Trickery and Other Methods Explored To Vanquish Potato Cyst Nematodes

20 Measuring the Potential of Switchgrass …


Cultural Practices To Maintain Soil Quality And Address Climate Change, Upendra Sainju, Dennis O’Brien Mar 2013

Cultural Practices To Maintain Soil Quality And Address Climate Change, Upendra Sainju, Dennis O’Brien

Agricultural Research Magazine

For decades, farmers in Montana and the Dakotas have produced impressive yields of barley and wheat. But that bounty has come at a cost. Tilling the soil in the region’s crop-fallow production systems has robbed the soil of nutrients and organic matter and reduced crop yields. In fact, the region’s soils have lost up to 50 percent of their organic matter in the last 50 to 100 years, and scientists say that current practices are unsustainable.

Agriculture also contributes about 25 percent of the human-made carbon dioxide and 70 percent of the human-made nitrous oxide being released into the atmosphere. …


Measuring The Potential Of Switchgrass Pellets, Paul Adler, Ann Perry Mar 2013

Measuring The Potential Of Switchgrass Pellets, Paul Adler, Ann Perry

Agricultural Research Magazine

President Barack Obama wants U.S. scientists to pursue an “all-of-the-above” strategy in developing new sources of domestic energy. Agricultural Research Service agronomist Paul Adler is providing complete cost-benefit breakdowns for using switch-grass pellets instead of fuel oil to heat homes and businesses in the Northeast.

“There have been a lot of studies on bioenergy potential,” says Adler, who works at the ARS Pasture Systems and Watershed Management Research Unit in University Park, Pennsylvania. “Most of them are focusing on transportation, but we still need a viable, commercial, bio-based fuel substitute for petroleum. In the meantime, our studies suggest that we …