Animal Sciences Commons^™

Analyses Of Whole-Genome Sequences From 185 North American Thoroughbred Horses, Spanning 5 Generations, Ernie Bailey, Carrie J. Finno, Jonah N. Cullen, Ted Kalbfleisch, Jessica Lynn Petersen

Department of Animal Science: Faculty Publications

Whole genome sequences (WGS) of 185 North American Thoroughbred horses were compared to quantify the number and frequency of variants, diversity of mitotypes, and autosomal runs of homozygosity (ROH). Of the samples, 82 horses were born between 1965 and 1986 (Group 1); the remaining 103, selected to maximize pedigree diversity, were born between 2000 and 2020 (Group 2). Over 14.3 million autosomal variants were identified with 4.5–5.0 million found per horse. Mitochondrial sequences associated the North American Thoroughbreds with 9 of 17 clades previously identified among diverse breeds. Individual coefficients of inbreeding, estimated from ROH, averaged 0.266 (Group 1) and …

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First Record Of The Diamond-Backed Watersnake (Nerodia Rhombifer) In Nebraska, Joseph W. Paysen, Keith Geluso, Daniel D. Fogell

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The diamond-backed Watersnake (Nerodia rhombifer) is a fairly large-bodied (≤2100g), semi-aquatic snake associated with wetlands throughout the south-central United States. Distribution of this species closely aligns with river floodplains in parts of its distribution, including the Missouri River floodplain as far north as Holt County, Missouri. Herein, we report on the first record of the Diamond-backed Watersnake in Nebraska. On May 21, 2011, Daniel D. Fogell collected a juvenile Diamond-backed Watersnake in southeastern Richardson County representing the first occurrence of the species in the state, which is adjacent to Holt County, Missouri. In extreme southeastern Nebraska, the distribution of this …

Correcting The Record: Subspecific Identification Of Nebraska’S Lone Common Eider (Somateria Mollissima), Sam Manning, Joel G. Jorgensen, Stephen J. Brenner, W. Ross Silcock

Nebraska Bird Review

In late November or early December 1967, Roger Guenther harvested a Common Eider (Somateria mollissima) on Maloney Canal between Maloney and Sutherland in Lincoln County, Nebraska. The head and a single foot from the carcass were deposited at the University of Nebraska State Museum via the University of Nebraska–Lincoln and the Nebraska Fish and Game Department (now the Game and Parks Commission). Dr. Jean Tate prepared the parts, which were inventoried as specimen #ZM-12341 in the UNSM. Dr. Paul Johnsgard purportedly took this specimen to the Field Museum of Natural History in Chicago for identification purposes, and concluded …

2023 (35th) Report Of The Nou Records Committee, Mark A. Brogie

Nebraska Bird Review

The functions and methods of the Nebraska Ornithologists’ Union Records Committee (NOURC) are described in its bylaws (NOURC 2010). The committee’s purpose is to provide a procedure for documenting unusual bird sightings and to establish a list of all documented birds for Nebraska. Species for which the NOURC seeks documentation (NOURC Review List) can be found at the NOU website https://noubirds.org/Birds/ReviewList.aspx. Starting in 2020, the NOURC decided to deal mainly with those species deemed as Accidental on the “Official List of the Birds of Nebraska”. Casual species and out-of- range species are now summarized in the “Seasonal Reports” of the …

The Nebraska Bird Review (September 2024) 92(3), Whole Issue

Nebraska Bird Review

Summer Field Report, June–July 2024, by W. Ross Silcock

Correcting the Record: Subspecific Identification of Nebraska’s Lone Common Eider (Somateria mollissima), by Sam Manning, Joel G. Jorgensen, Stephen J. Brenner, and W. Ross Silcock

2023 (35th) Report of the NOU Records Committee, by Mark A. Brogie

Subscription and Organization Information

Summer Field Report, June–July 2024, W. Ross Silcock

Nebraska Bird Review

This was an intriguing summer in several ways. I am far from being a knowledgeable ecologist, but, like most of us, as a layman I wonder whether and how weather affects birds, and if and how local weather is reflecting the conventional wisdom of climate change. Climate change seems increasingly to be expressed as various extreme or rapidly changing weather events that have differing effects on vegetation and consequently insect and larger prey populations. Every species responds differently it seems, depending on its biological needs, but food supply is the most important issue for birds. It is tempting to assign …

Technical Note: Assessing Gps Sensor Accuracy Using Real-Time Kinematic Device For Livestock Tracking, Biquan Zhao, Rebecca Mcdermott, Galen E. Erickson, Yijie Xiong

Department of Animal Science: Faculty Publications

The technical aspects of global positioning system (GPS) sensors have been improved substantially over the years, making them valuable and popular tools for livestock tracking. Using GPS sensors allows producers and researchers to locate grazing livestock, monitor their behavior and distribution, and gather referencing information about the health status of the animals and pastures. However, interpreting the behavior of grazing livestock, such as cattle, from GPS data can be difficult due to positioning inaccuracies. Without knowing the positioning accuracy range of GPS sensors, achieving high-level confidence in determining grazing densities and identifying abnormal livestock movement patterns is challenging. …

Knockdown Of Gonadotropin-Releasing Hormone Ii Receptor Impairs Ovulation Rate, Corpus Luteum Development, And Progesterone Production In Gilts, Amy T. Desaulniers, Rebecca Cederberg, Clay A. Lents, Brett White

Department of Animal Science: Faculty Publications

Reproduction is classically controlled by gonadotropin-releasing hormone (GnRH-I) and its receptor (GnRHR-I) within the brain. In pigs, a second form (GnRH-II) and its specific receptor (GnRHR-II) are also produced, with greater abundance in peripheral vs. central reproductive tissues. The binding of GnRH-II to GnRHR-II has been implicated in the autocrine/paracrine regulation of gonadal steroidogenesis rather than gonadotropin secretion. Blood samples were collected from transgenic gilts, with the ubiquitous knockdown of GnRHR-II (GnRHR-II KD; n = 8) and littermate controls (n = 7) at the onset of estrus (follicular) and 10 days later (luteal); serum concentrations of 16 steroid hormones …

Impact Of Partial Oil Removal On Energy Content Of Distillers Grains Plus Solubles For Finishing Cattle, Jordan E. Burhoop, J. L. Sperber, Curt J. Bittner, F. Henry Hilscher, J. C. Macdonald, Galen E. Erickson

Department of Animal Science: Faculty Publications

Two experiments evaluated the impact of the reduction in the percentage of corn oil remaining in distillers grains plus solubles (DGS) after the ethanol plant de-oiling process or by adding corn oil back to DGS following de-oiling on finishing cattle performance and nutrient digestion. Experiment 1 utilized 320 yearling steers (initial BW = 413 kg; SD = 25 kg) fed in 32 pens (10 steers/pen) and assigned to one of four treatments (n = 8 pens/treatment). The four treatments consisted of a blended DRC:HMC corn control diet (CON), de-oiled modified distillers grains plus solubles included at 40% of diet DM …

Impacts Of Invasive Carp And Their Population Dynamics On Fish Communities In The Missouri River, Joshua F. Kocik

School of Natural Resources: Dissertations, Theses, and Student Research

Invasive Carp species: Silver Carp Hypophthalmichthys molitrix, Bighead Carp Hypophthalmichthys nobilis, Grass Carp Ctenopharyngodon idella, and Black Carp Mylopharyngodon piceus have both potential and realized abilities to negatively impact native species. Specifically, Bighead Carp and Silver Carp as filter feeders have been theorized to be detrimental not only to adult filter-feeding species, but to many fish which rely on plankton and algae in their early-life stages. The impact of Invasive Carp on the overall fish community remains underexplored. The fish communities below Gavins Point Dam (Invasive Carp present) and Fort Randall Dam (Invasive Carp absent) afford the …

Spatiotemporal Plasticity In Reproductive Readiness And Recruitment Of Ichthyoplankton Of Invasive Silver Carp Along A Western Invasion Front, Jessi L. Urichich

School of Natural Resources: Dissertations, Theses, and Student Research

Individuals of invasive species at the edge of their invasion distribution may exhibit enhanced reproductive capacity and phenotypic plasticity leading to accelerated range expansion. Environmental conditions in highly fluctuating environments at distribution edges may either promote or hinder such reproductive readiness and phenotypic plasticity. Dynamic habitat conditions and periodic disturbances experienced in prairie streams, such as drought (e.g., low-flow, high water temperatures), may potentially reduce suitable spawning environments and ichthyoplankton recruitment of invasive Silver Carp, potentially slowing range expansion or enabling management strategies that reduce abundance in the absence of a reproducing population. Female Silver Carp batch fecundity, Gonadosomatic Index …

Investigating Associations Between Activity And Performance Traits Across Three Breeds Of Swine Using NuTrack, Dalton R. Obermier

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Feed costs are the largest contributor towards the variable costs of pork production. To decrease feed cost while not sacrificing output requires an improvement in feed efficiency (FE). A potential approach to expand available phenotypes associated with FE is with activity tracking, yet the relationship between activity and performance is not well defined in swine. Therefore, the objective of this study was to quantify associations between activity and feed intake traits. Average daily feed intake (ADFI) was collected on 1,766 purebred boars of three genetic lines (Duroc, Landrace, and Yorkshire) using FIRE (Osborne Industries, Inc., Osborne, Kansas) at a testing …

Understanding The Role Of The Microbiome In Infectious Disease, Developing Surveillance Tools, And Engineering Direct Fed Microbials To Improve Animal Health, Alison Clare (Bartenslager) Neujahr

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

With the ever-increasing demand for animal protein and the continual need for the assurance of proper animal welfare, investigations of preventative strategies for diseases is greatly needed. Specifically in the cattle industry, infectious bovine keratoconjunctivitis (IBK), commonly known as pinkeye, causes a large economic loss to the producer. However, effective mitigation strategies are lacking. Partly, this is due to the lack of studies investigating the ocular microbiome. Therefore, studies investigating the bovine ocular microbiome are greatly needed to better understand IBK disease etiology. To this end, we investigated the changes in the ocular microbiome in animals before, during and after …

Phylogenetic And Biogeographic Patterns Of Devonian Proetid Trilobites, Katherine Jane Jordan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Ecological disruption events such as mass extinctions can result in the permanent alteration in clades and ecosystems. A pivotal time of change during the history of life on Earth can be seen in the Devonian Period. During this time, major transitions in marine and terrestrial system resulted in a different world at the end of the period in comparison to the beginning. One such group most heavily impacted were trilobites. Trilobites were reduced to one order by the end of the Devonian: Proetida. My dissertation focuses on phylogenetic, biogeographic, and paleocommunity patterns of this group, known as the last of …

Information About Honey Fraud: Implications Of Information Choice For Consumer Behavior, Christopher R. Gustafson

Cornhusker Economics

The study highlights that standard research practice—exposing participants to information—may not capture what will happen outside of the lab when people have a choice about what to read, view, or do with their time. While we found that having participants read about honey fraud significantly shifted their willingness to pay for local and domestic versus imported honey, we did not find those changes when people were able to select a text to read.

The implications of these findings extend beyond the issue of honey fraud to broader contexts of food safety, nutrition, and production techniques. While people have preferences for …

Annual Forage Insurance Program Performance, Jay Parsons

Cornhusker Economics

In 2014, the Annual Forage Insurance Program (AFIP) was offered for the first time by the USDA – Risk Management Agency (RMA) as a pilot program for the states of Nebraska, North Dakota, South Dakota, Kansas, Oklahoma, and Texas. It was expanded to include Colorado in 2016 and New Mexico in 2017. This year marks the 10-year anniversary of the program, and the goal of this article is to provide a summary of where the program is and how it is being used. We will focus specific attention on Nebraska but also provide an overview of the national picture.

The …

Assessment Of Genetic Diversity And Population Structure Of U.S. Polypay Sheep From Breed Origins To Future Genomic Selection, Carrie S. Wilson, Jessica Lynn Petersen, Luiz F. Brito, Brad A. Freking, Sara M. Nilson, J. Bret Taylor, Thomas W. Murphy, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Knowledge of past and present genetic diversity within a breed is critical for the design and optimization of breeding programs as well as the development of strategies for the conservation of genetic resources. The Polypay sheep breed was developed at the U.S. Sheep Experiment Station (USSES) in 1968 with the goal of improving productivity in Western U.S. range flocks. It has since flourished in the more intensively managed production systems throughout the U.S. The genetic diversity of the breed has yet to be documented. Therefore, the primary objective of this study was to perform a comprehensive evaluation of the genetic …

Evaluation Of The Capacity And Effective Angle Of Thalamic Damage For 2 Commercially Available Captive Bolt Tool Types On Cadaver Heads From Sows >200 Kg Body Weight, Karly N. Anderson, Ruth Woiwode, Ashlynn A. Kirk, Jennifer Berger, Arquimides A. Reyes, Perle E. Zhitnitskiy, Kurt D. Vogel

Department of Animal Science: Faculty Publications

This study evaluated the ability of 2 penetrating captive bolt (PCB) types (PISTOL, INLINE) to reach and disrupt the thalamus when applied in 2 placements (FRONTAL, BEHIND EAR) to chilled cadaver heads (N = 60) from sows >200 kg. Heads were randomly distributed across 6 treatments (n = 10): FRONTAL-INLINE, FRONTAL-PISTOL, FRONTAL-NO SHOT, BEHIND EAR-INLINE, BEHIND EAR-PISTOL, and BEHIND EAR-NO SHOT. The FRONTAL shot was placed 3.5 cm superior to the optic orbits at the midline; the BEHIND EAR shot was placed directly caudal to the pinna of the ear on the same plane as the eyes and targeting the …

Biostratigraphic Revision Of Esthonyx (Tillidontia, Mammalia) In The Contect Of Climate Change In The Lower Eocene Of The Bighorn Basin, Wyoming, John Colter Johnson

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Esthonyx is an early Eocene tillodont found in North America, Europe, and India. The genus was named by Edward Drinker (E. D.) Cope in 1874 based on specimens from the San Jose Formation in New Mexico. Since then, several species of Esthonyx have been described from North America alone. The best record of Esthonyx comes from the central Bighorn Basin (BHB) in Wyoming, where it appears in 220 localities in a 640 m-thick stratigraphic succession. Since the last summary of Esthonyx in the BHB, several hundred new specimens have been collected and curated at the Denver Museum of Nature and …

Identifying The Relative Abundance Of Cell-Cell Signaling Peptides During Mammalian Hibernation And Anesthetic Administration By Label-Free Peptidomics Analysis, Somayeh Mousavi

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Cell-cell signaling molecules known as neuropeptide and peptide hormones, distributed in both the central nervous system (CNS) and the peripheral nervous system (PNS), regulate a variety of physiological processes including body temperature, feeding, circadian rhythm, reproduction, energy homeostasis, and more. While a number of active neuropeptides structures with physiological functions have been discovered, advances in peptidomic mass spectrometry are enabling researchers to uncover novel, previously unknown, active neuropeptide structures with potential for bioactivities. Using liquid chromatography coupled with tandem mass spectrometry (MS/MS), it is possible to identify a wide range of neuropeptides in single experiments without preselecting specific peptides. Mass …

Development Of A Durable And Low-Dose Universal Vaccine Against Swine Influenza A Virus, Erika Petro-Turnquist

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Swine Influenza A Virus (IAV-S) imposes a significant burden on the pork industry and poses a great threat to public health due to its zoonotic potential. Susceptibility to human, avian, and swine influenza make pigs the ultimate mixing vessels for reassorted influenza A virus strains. Current vaccination strategies induce short-lived and strain-specific responses against IAV-S. Further, the substantial genetic diversity of IAV-S necessitates consistent revaccination and updating of commercial vaccines. Computational platforms have recently emerged as a promising strategy to develop new-age vaccines against infectious diseases in livestock animals. Here, we describe a computationally derived set of vaccine immunogens, called …

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle Duncan Dougherty

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …

Population Demography, Occupancy, And Connectivity Of Invasive Bigheaded Carp In The Platte River, Nebraska And Its Tributaries, Blake W. Logan

School of Natural Resources: Dissertations, Theses, and Student Research

Silver Carp Hypophthalmichthys molitrix and Bighead Carp H. nobilis, collectively referred to as bigheaded carp, are invasive species in the United States of America that have expanded their range throughout the Missouri River basin in the last three decades. It may be desirable to develop management plans directed at the monitoring, control, or removal of bigheaded carp within the Missouri River basin. However, a knowledge gap exists regarding bigheaded carp ecology in Missouri River tributaries, which may be important for the development of management plans. As such, this study characterized bigheaded carp population demographics, occupancy, and connectivity in …

Virtual Fencing In Livestock Management: Effects On Beef Cattle Stress And Enhancing Wildlife Conservation, Kaitlyn E. Dozler

School of Natural Resources: Dissertations, Theses, and Student Research

I examined the effects of virtual fencing on beef cattle stress and how implementing the technology can aid wildlife habitat conservation at the University of Nebraska Gudmundsen Sandhills laboratory (GSL) near Whitman, NE. First, I examined how virtual fencing influences acute heart rate changes in beef cattle fitted with the virtual fencing collars. No significant difference in the average heart rate of a cow (P > 0.05) was observed between the 30 min control and virtual fenced periods for both trial dates. Second, I examined the influence of topography on wildlife species richness and presence at GSL using wildlife camera …

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle D. Dougherty

School of Natural Resources: Dissertations, Theses, and Student Research

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …

Hybridization Between The Rare Gray-Headed Chickadee And The Abundant Boreal Chickadee In The Midst Of Shifting Climate, Matthew R. Armstrong

School of Natural Resources: Dissertations, Theses, and Student Research

As species respond to changing climate, distributions and abundances may shift and alter species interactions. Hybridization, a relatively widespread phenomenon becoming more common with climate change, can have beneficial and detrimental effects on population growth rates and genetic integrity. Beneficial effects due to the introduction of advantageous alleles and increased genetic diversity may result from hybridization. Species may also accrue fitness costs associated with changing climates if mismatches occur between environmental variables and phenotypes. The gray-headed chickadee, Poecile cinctus lathami, is an extremely rare songbird that has experienced marked declines in recent decades within its restricted distribution in Alaska …

Heat Stress Changes The Bovine Methylome And Transcriptome And Investigation Of Two Novel Genetic Defects In Cattle, Rachel Renae Reith

Department of Animal Science: Dissertations, Theses, and Student Research

Heat stress is a major concern for livestock producers due to its negative impact on animal health and productivity. Heat stress does so by altering expression of genes through different regulatory mechanisms such as DNA methylation. Understanding how heat stress alters gene expression will help elucidate the genetic basis of physiological changes as well as identify targets for possible heat stress mitigation. The purpose of the first study was to understand how heat stress alters the adipose and skeletal muscle transcriptomes in zilpaterol-fed Brahman, as zilpaterol improves muscle growth and may mitigate the effects of heat stress. Differential expression and …

Heat Stress Changes The Bovine Methylome And Transcriptome And Investigation Of Two Novel Genetic Defects In Cattle, Rachel Renae Reith

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Heat stress is a major concern for livestock producers due to its negative impact on animal health and productivity. Heat stress does so by altering expression of genes through different regulatory mechanisms such as DNA methylation. Understanding how heat stress alters gene expression will help elucidate the genetic basis of physiological changes as well as identify targets for possible heat stress mitigation. The purpose of the first study was to understand how heat stress alters the adipose and skeletal muscle transcriptomes in zilpaterol-fed Brahman, as zilpaterol improves muscle growth and may mitigate the effects of heat stress. Differential expression and …

Daily Eicosapentaenoic Acid Infusion In Iugr Fetal Lambs Reduced Systemic Inflammation, Increased Muscle Adrβ2 Content, And Improved Myoblast Function And Muscle Growth, Haley Beer, Taylor A. Lacey, Rachel L. Gibbs, Micah S. Most, Zena M. Hicks, Pablo C. Grijalva, Eileen S. Marks-Nelson, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Intrauterine growth-restricted (IUGR) fetuses exhibit systemic inflammation that contributes to programmed deficits in myoblast function and muscle growth. Thus, we sought to determine if targeting fetal inflammation improves muscle growth outcomes. Heat stress-induced IUGR fetal lambs were infused with eicosapentaenoic acid (IUGR+EPA; n = 9) or saline (IUGR; n = 8) for 5 days during late gestation and compared to saline-infused controls (n = 11). Circulating eicosapentaenoic acid was 42% less (p < 0.05) for IUGR fetuses but was recovered in IUGR+EPA fetuses. The infusion did not improve placental function or fetal O2 but resolved the 67% greater (p < 0.05) circulating TNFα observed in IUGR fetuses. This improved myoblast function and muscle growth, as the 23% reduction (p < 0.05) in the ex vivo differentiation of IUGR myoblasts was resolved in IUGR+EPA myoblasts. Semitendinosus, longissimus dorsi, and flexor digitorum superficialis muscles were 24–39% lighter (p < 0.05) for IUGR but not for IUGR+EPA fetuses. Elevated (p < 0.05) IL6R and reduced (p < 0.05) β2 adrenoceptor content in IUGR muscle indicated enhanced inflammatory sensitivity and diminished β2 adrenergic sensitivity. Although IL6R remained elevated, β2 adrenoceptor deficits were resolved in IUGR+EPA muscle, demonstrating a unique underlying mechanism for muscle dysregulation. These findings show that fetal inflammation contributes to IUGR muscle growth deficits and thus may be an effective target for intervention.