Animal Sciences Commons^™

Impact Of Reducing Ammonia Concentration By Ozone Technology And Its Potential Improvement On Broiler Performance, Odilpio Quintana Ortega

Graduate Theses and Dissertations

The employment of ozone in livestock production as a means of mitigating ammonia is used for enhancing air quality within animal confinement facilities, such as swine or poultry barns. Ammonia is a byproduct of animal excrement. Elevated ammonia concentrations can adversely affect the well-being of both animals and humans. The aim of this thesis was to investigate the effects of Ozone Technology Machine Unit (OTMU) utilization in poultry barns regarding ammonia concentration and its potential benefit in terms of broilers performance and welfare. In recent years, there has been a significant boom in technological advances. New devices that help mitigate …

Demographics, Sexual Dimorphism, And Ecological Aspects Of Ambystoma Annulatum (Ringed Salamander) In Northwest Arkansas, Usa, Brian M. Becker

Graduate Theses and Dissertations

The need to study and monitor amphibian populations is increasing along with the threats to their population stability and persistence in nature. Northwest Arkansas is one of the fastest growing areas in the nation and with that growth comes rapid changes in land use, massive alterations to habitats, habitat loss, and the introduction of nonnative plants and animals. Ambystoma annulatum (Ringed Salamander) is an Ambystomatid endemic to the Ozark and Ouachita Mountains of southern Missouri, northern and western Arkansas, and eastern Oklahoma giving it a relatively small distribution compared to most Ambystoma. Therefore, Arkansas constitutes a significant portion of the …

Assessment Of Dietary Energy And Amino Acid Diet Formulation In The Modern Broilers, Matheus Freitas Costa

Graduate Theses and Dissertations

As a result of the advancement of genetics, understanding broiler requirements for dietary energy and amino acids is essential for improving live performance and carcass characteristics. The energy system used to formulate diets in poultry is based on metabolizable energy (ME); this equation can be described as the total amount of energy in the feed minus gases and feces. However, the net energy (NE) system may be the best depiction of the energy utilized by birds for production and maintenance purposes once the heat increment (HI) is measured. Thus, a series of studies were conducted to establish the responsiveness of …

The Effects Of Probiotics And Environment On The Swine Gut Microbiome, Bin Zuo

Graduate Theses and Dissertations

The gastrointestinal tract is crucial for nutrient absorption and immune system functionality. It employs a synergistic approach, merging physical and biochemical defenses, to counteract pathogens that enter through dietary or other external routes. This system also facilitates the breakdown of complex carbohydrates, a process beyond the host's innate capacity. In swine, gut microbiota early colonization plays a significant role in several physiological developments, shaped by a variety of determinants including maternal factors and environmental conditions. In the swine industry, the shift to indoor pig farming enhances efficiency but limits soil interaction, potentially impacting gut microbiota diversity. Additionally, the practice of …

Controlling Salmonella: Strategies For Feed, The Farm, And The Processing Plant, Tomi Obe, Timothy Boltz, Mike Kogut, Steven C. Ricke, Lasheda A. Brooks, Ken Macklin, Ashley Peterson

Poultry Science Faculty Publications and Presentations

Controlling Salmonella in poultry is an ongoing food safety measure and while significant progress has been made, there is a need to continue to evaluate different strategies that include understanding Salmonella-poultry interaction, Salmonella-microbiota interactions, Salmonella genetics and response to adverse conditions, and preharvest and postharvest parameters that enable persistence. The purpose of this symposium is to discuss different strategies to consider from feed milling to the farm to the processing environment. This Poultry Science Association symposium paper is divided into 5 different sections that covers 1) immunological aspects of Salmonella control, 2) application of Salmonella genetics for targeted …

Innovative Approaches To Poultry Processing Wastewater Treatment: The Stainless Steel Ultrafiltration Membrane As A Viable Option, Saubana Olorunrola Dada, Chidambaram Thamariselvan, Mahmood Jebur, Sumith Ranil Wickramasinghe

Chemical Engineering Faculty Publications and Presentations

In pursuit of sustainability, we explored replacing conventional dissolved air floatation (DAF) in poultry processing wastewater (PPW) treatment with a precisely tuned 0.02 µm stainless-steel ultrafiltration (SSUF) membrane. SSUF is a robust, homogenously porous membrane with strong chemical resistance, ease of cleaning, and exceptional resistance to organic fouling. Unlike polymeric membranes, it can be regenerated multiple times, making it a cost-effective choice due to its compatibility with harsh chemical cleaning. The PPW used for the study was untreated wastewater from all processing units and post-initial screening. Our study revealed the SSUF membrane’s exceptional efficiency at eliminating contaminants. It achieved an …

Editorial: Respiratory Microbiome In Health And Disease, Yimin Zhuang, Tao Ding, Jiangchao Zhao, Jianmin Chai

Animal Science Faculty Publications and Presentations

Editorial on the Research Topic:

Respiratory microbiome in health and disease

The Spatial Dissimilarities And Connections Of The Microbiota In The Upper And Lower Respiratory Tract Of Beef Cattle, Zhihao Zhang, Chengqian Zhang, Yikai Zhong, Shuli Yang, Feilong Deng, Ying Li, Jianmin Chai

Animal Science Faculty Publications and Presentations

Bovine respiratory disease (BRD) causes morbidity and mortality in cattle. The critical roles of the respiratory microbiota in BRD have been widely studied. The nasopharynx was the most popular sampling niche for BRD pathogen studies. The oral cavity and other niches within the respiratory tract, such as nostrils and lung, are less assessed. In this study, oropharyngeal swabs (OS), nasal swabs (NS), nasopharyngeal swabs (NP), and bronchoalveolar lavage (BAL) were collected from calves located in four countries and analyzed for investigation of the dissimilarities and connections of the respiratory microbiota. The results showed that the microbial diversity, structure, and composition …

An Amplification-Free Crispr-Sers Biosensor For Specific, Sensitive And Rapid Detection Of Salmonella Typhimurium In Poultry, Fei Jia, Bingyan Li, Yawen He, Yafang Shen, Juhong Chen, Xingmin Li, Yanbin Li

Biological and Agricultural Engineering Faculty Publications and Presentations

CRISPR (Clustered regularly interspaced short palindromic repeats) has been a cutting-edge biotechnology in both gene editing and biosensing due to its desirable features, such as high base resolution and set-and-forget operation. However, as a nucleic acid detection method, an DNA/RNA amplification procedure is still inevitable in CRISPR biosensors. In this study, a highly-ordered nanopore array SERS substrate, coated with silver nanoparticles, was fabricated and utilized as a platform for the specific detection of Salmonella Typhimurium (S. Typhimurium) without the time-consuming DNA amplification step. The smart sensing strategy was established by introducing an ssDNA labeled with a Raman active molecule …

Characterizing The Impact Of Enterococcus Cecorum Infection During Late Embryogenesis On Disease Progression, Cecal Microbiome Composition, And Early Performance In Broiler Chickens, Marcela Arango, Aaron Forga, Jing Liu, Latasha Gray, Randy Moore, Makenly E. Coles, Abdiel Atencio, Carolina Trujillo, Juan David Latorre, Guillermo Tellez-Isaias, Billy Hargis, Danielle Graham

Poultry Science Faculty Publications and Presentations

Enterococcus cecorum (EC) has been associated with septicemia and early mortality in broiler chickens. There is limited research investigating the pathogenicity of EC field strains obtained from affected birds. The purpose of this study was to evaluate the effect of in-ovo administration into the amnion with different EC field isolates at d 18 of embryogenesis (DOE18). In Exp 1, 7 EC field isolates alone or in combination (EC1–EC3, EC4–EC5, EC6, and EC7) were selected based on phenotypic characteristics and evaluated at different concentrations (1 × 10², 1 × 10⁴, and 1 × …

Understanding Functional Benefits Of Isoleucine And Arginine On Poultry Performance And Health., Roshan Adhikari, Jason T. Lee, Keith Haydon

Proceedings of the Arkansas Nutrition Conference

Poultry industry is rapidly moving towards utilizing the best ingredients to precisely feed the birds for better performance, welfare, health, and profitability. One of the practices that evolved over time with research is better optimization of amino acid utilization in the diets thus changing the crude protein landscape. Historically, if we compare the modern diets to diets from 1980, significant changes are visible in the use of unbound or crystalline amino acids leading to reduced levels of CP and reduced unutilized nitrogen in the diets. Multiple feed-grade amino acids are available in a cost-effective manner in poultry diets. Multiple research …

The Himalayan Uplift And Evolution Of Aquatic Biodiversity Across Asia: Snowtrout (Cyprininae: Schizothorax) As A Test Case, Binod Regmi, Marlis R. Douglas, Karma Wangchuk, Zachery D. Zbinden, David R. Edds, Singye Tshering, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

Global biodiversity hotspots are often remote, tectonically active areas undergoing climatic fluctuations, such as the Himalaya Mountains and neighboring Qinghai-Tibetan Plateau (QTP). They provide biogeographic templates upon which endemic biodiversity can be mapped to infer diversification scenarios. Yet, this process can be somewhat opaque for the Himalaya, given substantial data gaps separating eastern and western regions. To help clarify, we evaluated phylogeographic and phylogenetic hypotheses for a widespread fish (Snowtrout: Cyprininae; Schizothorax) by sequencing 1,140 base pair of mtDNA cytochrome-b (cytb) from Central Himalaya samples (Nepal: N = 53; Bhutan: N = 19), augmented with 68 GenBank …

Design, Fabrication, And Evaluation Of 3-D-Printed Cystotomy Spoons As A Retrieval Method In Dogs, Davina C. D'Angelo, Sam E. Stephens, Morten O. Jensen, Lauren R. Thomas

Animal Science Faculty Publications and Presentations

OBJECTIVE

Current cystotomy methods often implement the use of off-label devices, resulting in urocystolith extraction difficulty and potentially leading to postoperative complications and discomfort for the patient. The objective of this study was to create 3 novel 3-D printed cystotomy spoons that offer a dedicated solution for removing urocystoliths from a patient's urinary bladder.

ANIMALS

Clinical use of the 3 novel 3-D printed cystotomy spoons were ultimately evaluated in 4 dogs and 1 cat that presented for urocystotlith removal at 3 different veterinary hospitals in northwest Arkansas.

METHODS

The novel cystotomy spoons were designed using SolidWorks, 3-D printed with a …

Editorial: Parasitism: The Good, The Bad And The Ugly, Marco A. Juarez-Estrada, Danielle Graham, Xochitl Hernandez-Velasco, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

Editorial on the Research Topic

Parasitism: the good, the bad and the ugly

Potent Bile Acid Microbial Metabolites Modulate Clostridium Perfringens Virulence, Tahrir Jatli Alenezi, Ying Fu, Bilal Alrubaye, Thamer Alanazi, Ayidh Almansour, Hong Wang, Xiaolun Sun

Poultry Science Faculty Publications and Presentations

Clostridium perfringens is a versatile pathogen, inducing diseases in the skin, intestine (such as chicken necrotic enteritis (NE)), and other organs. The classical sign of NE is the foul smell gas in the ballooned small intestine. We hypothesized that deoxycholic acid (DCA) reduced NE by inhibiting C. perfringens virulence signaling pathways. To evaluate the hypothesis, C. perfringens strains CP1 and wild-type (WT) HN13 and its mutants were cultured with different bile acids, including DCA and isoallolithocholic acid (isoalloLCA). Growth, hydrogen sulfide (H₂S) production, and virulence gene expression were measured. Notably, isoalloLCA was more potent in reducing growth, H …

Isolation, Characterization, And Experimental Infection Of Streptococcus Gallolyticus Subspecies Pasteurianus From Commercial Turkeys With Acute Septicemia: A Pilot Study, L. S. Gray, J. D. Latorre, D. Hernandez-Patlan, B. Solis-Cruz, V. Petrone-Garcia, X. Hernandez-Velasco, K. M. Robbins, R. W. Moore, C. N. Vuong, A. Stein, L. Laverty, K. Martin, M. E. Coles, R. Señas-Cuesta, J. M. Diaz-Gomez, I. Loeza, I. Castellanos-Huerta, J. A. Maguey-Gonzalez, B. D. Graham, B. M. Hargis, G. Tellez-Isaias

Poultry Science Faculty Publications and Presentations

Streptococcus gallolyticus (SG) is a Gram-positive cocci found as commensal gut flora in animals and humans. SG has emerged as a cause of disease in young poults between 1 and 3 wk of age. SG is associated with septicemia resulting in acute mortality with no premonitory signs in turkeys. Three SG isolates were obtained from clinical field cases of acute septicemia of commercial turkeys and used in three independent experiments. In Experiment 1, embryos were inoculated 25 d of embryogenesis with varying concentrations of SG1, SG2, or SG3. In Experiment 2, day of hatch, poults were inoculated with varying concentrations …

Feed Formulation: Expanding Our Horizons Beyond The Simple Provision Of Least-Cost Diets, Rick Kleyn, Mariana Ciacciariello

Proceedings of the Arkansas Nutrition Conference

Commercial feed formulation is typically based on linear programming (LP) algorithms that derive a least-cost solution for a blend of ingredients that meet specific nutrient criteria. This methodology has been effectively used for decades, but how we achieve our primary goal, which is sustainable poultry production, needs to be reconsidered in broader terms. Some of the issues that this paper will deal with are: not all parameters that we consider are linear and additive; we are still unsure of how to build the matrix required for feed formulation; dealing with the variability encountered in every batch of ingredient remains a …

Reducing Feed’S Environmental Footprint: Where Are The Opportunities?, Lara Moody

Proceedings of the Arkansas Nutrition Conference

Working with animal food industry members and stakeholders up- and down-stream in the supply chain, the Institute for Feed Education and Research (IFEEDER) developed a ‘Sustainability Road Map’ to support the U.S. animal food industry as it pursues continuous improvement on impacts important to industry members and their customers. For IFEEDER and the American Feed Industry Association (AFIA), sustainability is defined and managed by each individual organization to deliver measurable, continuous improvements on the impacts related to people, planet and governance that are most important to them and their stakeholders.

No Antibiotic Ever (Nae) Versus Conventional Broiler Production: It’S Complicated, Marcos H. Rostagno

Proceedings of the Arkansas Nutrition Conference

Broiler production has evolved significantly over the years to meet a constant growing demand for animal protein. Rooted on major advances in genetics, facilities and environmental conditions, management, and specially, in nutrition and health, the U.S. broiler industry improved tremendously in productivity and efficiency, clearly demonstrated by a marked reduction of the average market age (i.e., days to market), while significantly increasing average market weight, breast meat yield and feed efficiency (National Chicken Council, 2023). For decades, conventional broiler production was dominant, with extensive use of antibiotics to promote growth and manage disease challenges (prevention and control). However, concerns over …

Organic Trace Minerals In Environmentally Sustainable Systems, Sergio L. Vieira, Julmar C. Feijó, André Favero

Proceedings of the Arkansas Nutrition Conference

Essential trace minerals (Zn, Cu, Fe, Mn, Se and I) are traditionally included as supplements through mineral premixes in poultry feeds. In comparison to other essential nutrients, as well as energy, they are scarcely studied. The essentiality of a nutrient, by definition, refers to the nutrient role in normal body function, such as growth or egg production, and considers that they cannot be synthesized by the body and, therefore, must be supplied from the feed.

Application Of New Molecular Tools To Assess Virus Viability In Production Animals, Declan C. Schroeder

Proceedings of the Arkansas Nutrition Conference

Persistent viral infections routinely impose an inconvenient, always costly but thankfully not all too often catastrophic outcome on our modern food system. Managing viruses is key for all concerned if our objective is to meet the ever-growing demand to high-value animal protein. Whether it’s the impact influenza A virus (IAV) has on the avian or swine industries, Porcine reproductive and respiratory virus (PRRSV) on the swine or Deformed wing virus (DWV) on honey bees in both the honey and pollinator sectors - the negative impact and damaging cost to the individual producer, country and global economy cannot be overstated.

A View Of Poultry Production In The Americas, Michael Donohue

Proceedings of the Arkansas Nutrition Conference

The primary goal of the presentation is to share a long-term overview of how broiler production has changed over the last 35 years in areas of bird performance, production costs, particularly in the area of feed ingredient cost per lb. and illustrating how poultry nutritionists have adapted feed formulations to meet the changes in performance and cost.

Roost Selection By Synanthropic Bats In Rural Kenya: Implications For Human-Wildlife Conflict And Zoonotic Pathogen Spillover, Reilly T. Jackson, Paul W. Webala, Joseph G. Ogola, Tamika J. Lunn, Kristian M. Forbes

Biological Sciences Faculty Publications and Presentations

Many wildlife species are synanthropic and use structures built by humans, creating a high-risk interface for human–wildlife conflict and zoonotic pathogen spillover. However, studies that investigate features of urbanizing areas that attract or repel wildlife are currently lacking. We surveyed 85 buildings used by bats and 172 neighbouring buildings unused by bats (controls) in southeastern Kenya during 2021 and 2022 and evaluated the role of microclimate and structural attributes in building selection. We identified eight bat species using buildings, with over 25% of building roosts used concurrently by multiple species. Bats selected taller cement-walled buildings with higher water vapour pressure …

Effect Of Bovine Respiratory Disease On The Respiratory Microbiome: A Meta-Analysis, Samantha Howe, Beth Kegley, Jeremy Powell, Sicheng Chen, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

Background: Bovine respiratory disease (BRD) is the most devastating disease affecting beef and dairy cattle producers in North America. An emerging area of interest is the respiratory microbiome’s relationship with BRD. However, results regarding the effect of BRD on respiratory microbiome diversity are conflicting.

Results: To examine the effect of BRD on the alpha diversity of the respiratory microbiome, a meta-analysis analyzing the relationship between the standardized mean difference (SMD) of three alpha diversity metrics (Shannon’s Diversity Index (Shannon), Chao1, and Observed features (OTUs, ASVs, species, and reads) and BRD was conducted. Our multi-level model found no difference in Chao1 …

Alkaline Pretreatment And Air Mixing For Improvement Of Methane Production From Anaerobic Co-Digestion Of Poultry Litter With Wheat Straw, Yuanhang Zhan, Jun Zhu, Yiting Xiao, Leland C. Schrader

Biological and Agricultural Engineering Faculty Publications and Presentations

Alkaline pretreatment (AL) and air mixing (air) both have the potential to improve anaerobic co-digestion (Co-AD) of poultry litter with wheat straw for methane production. In this study, the effects of the combination of AL (pH 12 for 12 h) and air mixing (12 mL·d⁻¹) on the Co-AD process were investigated. The substrate hydrolysis was enhanced by AL, with soluble chemical oxygen demand increased by 4.59 times and volatile fatty acids increased by 5.04 times. The cumulative methane yield in the group of Co-AD by AL integrated with air (Co-(AL + air)), being 287 mL·(g VS_added) …

Evaluation Of Clostridium Septicum Hemolytic Activity, Administration Route, And Dosage Volume Of A Clostridial Dermatitis (Cellulitis) Bacterin-Toxoid On Humoral Immune Response In Commercial Turkeys, Aaron Forga, Kabel Robbins, Andrew Smith, Makenly Coles, Guillermo Tellez-Isaias, Christine N. Vuong, Billy Hargis, Danielle Graham

Poultry Science Faculty Publications and Presentations

Clostridial cellulitis or dermatitis affects commercial turkey flocks, primarily as they approach market age. In the field, this disease has been effectively controlled with antibiotics, but alternatives to antibiotics are needed. Bacterin-toxoid vaccination programs have been shown to prevent clostridial diseases in other species, including humans. Results from previous field studies indicate that vaccination with an experimental whole-cell Clostridium septicum (CS) bacterin-toxoid oil emulsion vaccine reduced clostridial dermatitis-associated mortality and antibiotic usage for some commercial turkey flocks, but vaccination was not always efficacious. To improve vaccine efficacy, studies were conducted to optimize the antigenic component of the experimental …

Effects Of Supplemental Trace Minerals As Amino Acid Complexed Or Inorganic Sources For Beef Cattle From Receiving Through Finishing, Robin Ashley Cheek

Graduate Theses and Dissertations

The objective of experiments was to investigate the effects of inorganic or amino acid-complexed sources of trace minerals on health, gain performance, liver mineral concentrations, and carcass characteristics of beef heifers from receiving through finishing. To investigate the effects of inorganic or amino acid-complexed sources of trace minerals (zinc, copper, manganese, and cobalt) on performance and morbidity of beef heifers during the receiving period, crossbred beef heifer calves arriving on 3 delivery dates were used in a 42-day receiving trial. Treatments consisted of supplemental zinc (360 mg/d), copper (125 mg/d), manganese (200 mg/d), and cobalt (12 mg/d) from complexed (Availa-4, …

Evaluation Of Heat Stress And Dietary Amino Acid Density On Nutrient And Energy Partitioning In Broilers, Jean-Remi Teyssier

Graduate Theses and Dissertations

The simultaneous increase in the global demand for animal-based protein products and the rise in temperature associated with global warming pose important challenges to the poultry industry. Heat stress (HS) occurs when an animal’s heat production (HP) surpasses its capacity to dissipate heat into the surrounding environment, ultimately leading to reduced feed intake (FI) and negative impacts on performance. A better understanding of bird responses to elevated temperatures is required to propose effective nutritional strategies that mitigate the adverse effects of HS. Therefore, this dissertation aimed to characterize the nutritional and physiological mechanisms explaining the loss in performance and carcass …

Investigation Of Consumer Freezing Practices, Condition And Duration On Palatability Of Beef, Elizabeth Sue Neal

Graduate Theses and Dissertations

The objective of these studies was to determine: the impact of extended periods of frozen storage duration and packaging type on palatability traits of cooked beef steaks from two muscles and the impact of freezing type and storage duration on objective and subjective measures of ground beef palatability traits. In study 1, no differences were elicited from any of the possible interactions (P= 0.95) for SSF or CL: package × muscle × freezing duration, package × muscle, package × duration or muscle × duration. However, freezing duration did impact both SSF (P < 0.01) and CL (P < 0.001). Generally, for both SSF and CL steaks that were frozen for 9-months elicited both the highest SSF values as well as the greatest percentage of CL among all treatments. In comparison, EM was impacted by the interaction (P = 0.059) of packaging type  muscle  freezing duration. Samples from OW GM stored for 9-months elicited the highest percentage of EM while fresh OW LL samples resulted in the lowest. Additionally, expressible moisture was impacted by the interaction (P = 0.047) of muscle  freezing duration. Among all treatments, GM steaks that were frozen for 9-months elicited the highest EM values and fresh LL samples the lowest EM values. Contrastingly, there was no significant difference observed in expressible moisture for the interaction (P  0.18) of package type  freezing duration or for the interaction (P = 0.70) of package type  muscle. Generally, fresh OW GM steaks resulted in the greatest concentration of lipid derived volatile compounds such as aldehydes, hydrocarbons and alcohols which contrasted sensory ratings of trained panelists that indicated oxidized and refrigerator-stale ratings increased as storage time increased for OW steaks. In study 2, The interaction of freezer treatment × storage duration impacted gumminess (P = 0.05), a TPA attribute. In greater detail, samples stored in RF for 6-months resulted in the greatest gumminess values (P < 0.001), while those stored in the CF for 12-months elicited the lowest (P < 0.001). Similarly, flavor development was also impacted by the interaction (P = 0.05) of freezer treatment × storage duration. Three lipid derived compounds were of greatest concentration among RF patties stored for 1-month. In contrast, the interaction of freezer treatment × storage duration elicited no impact on consumer ratings, SF or TBARS. Nonetheless, frozen storage duration impacted TPA, flavor development, consumer ratings, SF and TBARS as a main effect (P < 0.05), especially in regard to tenderness and juiciness. Moreover, beef flavor development, tenderness and juiciness are impacted by freezing duration, muscle, freezer type and packaging, however these factors are not necessarily independent of one another. Furthermore, the retail display period is a critical period for fresh beef steaks, especially when packaged in aerobic conditions. For optimal eating experience, beef products should be stored for extended periods in a vacuum packaging, in a designated freezer that is not opened frequently to allow for optimal air flow regulation and minimize freezer burn to improve tenderness and juiciness.

Dietary Histidine: Broiler Responsiveness With And Without Endogenous Histidine Reserve Consideration, Kenneth Blake Nelson

Graduate Theses and Dissertations

In the past, histidine warranted little concern from poultry nutritionists because it was seldom deficient in practical broiler diets. With most essential amino acids now commercially available as feed-grade forms to partially replace intact protein ingredients, histidine and its minimum in least-cost formulation are receiving fresh attention. Published histidine requirement data for broilers are sparce, and many of these studies have been conducted on males within the first three weeks post-hatch. Moreover, rudimentary dose-response experimental designs may not be applicable to estimate histidine requirements in later growth periods because histidine is uniquely stored within the body. In times of dietary …