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South Dakota State University

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Articles 571 - 600 of 1392

Full-Text Articles in Animal Sciences

Nitrogen Excretion From Beef Cattle For 6 Cover Crop Mixes As Estimated By A Nutritional Model, E.E. Grings, A. Sackey, M. Hansen, V. Owens, D. Beck, P. Sexton Jan 2013

Nitrogen Excretion From Beef Cattle For 6 Cover Crop Mixes As Estimated By A Nutritional Model, E.E. Grings, A. Sackey, M. Hansen, V. Owens, D. Beck, P. Sexton

South Dakota Beef Report, 2013

Excretion of nitrogen (N) from cattle within crop‐livestock systems is an important component of nutrient cycling, but measuring fecal and urinary N excretion in grazing cattle is a difficult and time consuming task. Nutritional models are available to estimate feed utilization and have been used to predict N excretion in grazing cattle. Using the Large Ruminant Nutrition Model, we predicted N losses from mature pregnant beef cows and growing beef heifers from compositional analysis of cover crop mixes grown in central South Dakota. All of the mixes used contained crude protein (CP) concentrations greater than cattle requirements. Estimates of both …


Overwinter Mortality Of Sympatric Juvenile Bluegill And Yellow Perch In Mid-Temperate Sandhill Lakes, Nebraska, U.S.A, Jeffrey C. Jolley, Mark A. Kaemingk, David W. Willis, Richard S. Holland Jan 2013

Overwinter Mortality Of Sympatric Juvenile Bluegill And Yellow Perch In Mid-Temperate Sandhill Lakes, Nebraska, U.S.A, Jeffrey C. Jolley, Mark A. Kaemingk, David W. Willis, Richard S. Holland

Natural Resource Management Faculty Publications

Substantial mortality can occur in age-0 fish populations during their first year of life, especially in winter; this can potentially influence overall recruitment into the adult population. As such, we compared relative abundances between fall and spring catches of sympatric juvenile bluegill Lepomis macrochirus Rafinesque and yellow perch Perca flavescens (Mitchill) to evaluate the magnitude of overwinter mortality across locations (five lakes for two years) and through time (one lake for six years). In addition, we compared both quantile-quantile and increment plots, based on length-frequency histograms from fall- and spring-caught cohorts from 2004 to 2010, to determine if mortality was …


Growing South Dakota (Winter 2013), College Of Agriculture &. Biological Sciences Jan 2013

Growing South Dakota (Winter 2013), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2012 South Dakota Agricultural Station Annual Report

CONTENTS:
[Page] 1 From The Dean
[Page] 2 Commitment To Collaboration: Working Together Adds Efficiency To Budgets: Effectiveness To Solutions
[Page] 7 Special Pull-Out Section: South Dakota Agricultural Experiment Station Annual Report: Highlighting Research Projects On Beef; Wheat; And Soybean Meal For Aquaculture
[Page] 11 Water Watchers: The South Dakota Water Resources Institute Provides Research, Education & Outreach
[Page] 12 Winter College News
[Page] 14 SDSU Extension: One Year Later: Reorganization Prompted Restructuring – And Renewed Commitment
[Page] 16 Bullseye!: South Dakota’s 4-H Shooting Sports Program Sees Continued Growth …


Managing Annual Cow Costs, Julie Walker Jan 2013

Managing Annual Cow Costs, Julie Walker

Animal Science Faculty Publications

Published in Proceedings of the 23rd Range Beef Cow Symposium- Rapid City, SD.


News Of The Department Of Animal Science, Department Of Animal Science Dec 2012

News Of The Department Of Animal Science, Department Of Animal Science

Animal Science Newsletters

No abstract provided.


Growing South Dakota (Fall 2012), College Of Agriculture &. Biological Sciences Oct 2012

Growing South Dakota (Fall 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Kudos To South Dakota Farmers: SDSU Study Credits Farmers For Conserving Soil, Sequestering More Carbon
[Page] 4 Fall College News
[Page] 6 Investing In Research: Additional Appropriations Being Sought For SDSU’s Ag Station Research
[Page] 8 Pertinent Publications: Plant Science Department Produces Several New Guide Books
[Page] 9 Special Pull-Out Section: Snapshot from SDSU’s College of Agriculture & Biological Sciences: Highlighting Academic Programs, Ag Experiment Station and SDSU Extension
[Page] 13 Plans Proceed: Efforts For Cow/Calf Training And Research Center Continue
[Page] 14 Outreach Through Horses: Initiative Teaches Equine Care On South Dakota Reservations
[Page] 16 Here And …


Foraging Ecology Of Fall-Migrating Shorebirds In The Illinois River Valley, Randolph V. Smith, Joshua Stafford, Aaron P. Yetter, Michelle M. Horath, Christopher S. Hine, Jeffery P. Hover Sep 2012

Foraging Ecology Of Fall-Migrating Shorebirds In The Illinois River Valley, Randolph V. Smith, Joshua Stafford, Aaron P. Yetter, Michelle M. Horath, Christopher S. Hine, Jeffery P. Hover

Natural Resource Management Faculty Publications

Populations of many shorebird species appear to be declining in North America, and food resources at stopover habitats may limit migratory bird populations. We investigated body condition of, and foraging habitat and diet selection by 4 species of shorebirds in the central Illinois River valley during fall migrations 2007 and 2008 (Killdeer [Charadrius vociferus], Least Sandpiper [Calidris minutilla], Pectoral Sandpiper [Calidris melanotos], and Lesser Yellowlegs [Tringa flavipes]). All species except Killdeer were in good to excellent condition, based on size-corrected body mass and fat scores. Shorebird diets were dominated by invertebrate taxa from Orders Diptera and Coleoptera. Additionally, Isopoda, Hemiptera, …


News Of The Department Of Animal Science, Department Of Animal Science Aug 2012

News Of The Department Of Animal Science, Department Of Animal Science

Animal Science Newsletters

No abstract provided.


News Of The Department Of Animal Science, Department Of Animal Science Jul 2012

News Of The Department Of Animal Science, Department Of Animal Science

Animal Science Newsletters

No abstract provided.


Survival Of White-Tailed Deer Fawns In The Grasslands Of The Northern Great Plains, Troy W. Grovenburg, Robert W. Klaver, Jonathan A. Jenks Jul 2012

Survival Of White-Tailed Deer Fawns In The Grasslands Of The Northern Great Plains, Troy W. Grovenburg, Robert W. Klaver, Jonathan A. Jenks

Natural Resource Management Faculty Publications

Environmental factors, such as forest characteristics, have been linked to fawn survival in eastern and southern white-tailed deer ( Odocoileus virginianus) populations. In the Great Plains, less is known about how intrinsic and habitat factors influence fawn survival. During 2007-2009, we captured and radiocollared 81 fawns in north-central South Dakota and recorded 23 mortalities, of which 18 died before 1 September. Predation accounted for 52.2% of mortality; remaining mortality included human (hunting, vehicle, and farm accident; 26.1%) and hypothermia (21.7%). Coyotes (Canis latrans) accounted for 83.3% of predation on fawns. We used known-fate analysis in Program MARK to estimate summer …


Growing South Dakota (Summer 2012), College Of Agriculture &. Biological Sciences Jul 2012

Growing South Dakota (Summer 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 150 Years and Counting: 2012 Marks a Significant Milestone for Land-Grant Universities [Page] 4 Summer College News
[Page] 6 Ready, Set, Grow: Gardening Programs Offer bevy of Benefits to Community
[Page] 8 SDSU Extension Staff Directory: Pull Out and Save for Future Reference
[Page] 13 Bugs Beware!: SDSU Assembles Talented Team of Entomologists
[Page] 14 High-Tech Teaching: Curriculums Now Integrate iPads, Social Media & Peer Mentoring
[Page] 16 A Lesson in Chinese: SDSU Contingent Experiences World's Largest Marketplace
[Page] 18 Here and There
[Page] 20 Adapting with Apps: Technological Advancements Offer Opportunities for Farming's Future
[Page] 21 A …


News Of The Department Of Animal Science, Department Of Animal Science Apr 2012

News Of The Department Of Animal Science, Department Of Animal Science

Animal Science Newsletters

No abstract provided.


Growing South Dakota (Spring 2012), College Of Agriculture &. Biological Sciences Apr 2012

Growing South Dakota (Spring 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Powerful Potential: Growing Global Population Spurs Need for Agbioscience
[Page] 4 Spring College News
[Page] 6 Enhanced Education: Students Complement Coursework With Extra-Curricular Opportunities
[Page] 8 Gaining Global Perspective: SDSU Delegation Visits Argentina
[Page] 9 Creative Collaboration: Students, Landowners & Industry Professionals Pair Up for a Positive Experience
[Page] 10 Wondrous Wheat: New Research Agreement & Wheat Varieties Announced
[Page] 12 Here and There
[Page] 14 New Era Underway: SDSU Extension Re-Energizes Programs, Delivery [Page] 16 iGrow Continues to Grow
[Page] 18 Focused on the Future: Field Specialists Strive to Serve Changing Landowner Needs
[Page] 19 Connecting with …


Causes Of Pneumonia Epizootics Among Bighorn Sheep, Western United States, 2008–2010, Thomas E. Besser, Margaret A. Highland, Katherine Baker, E. Frances Cassirer, Neil J. Anderson, Jennifer M. Ramsey, Kristen Mansfield, Darren L. Bruning, Peregrine Wolff, Joshua B. Smith, Jonathan A. Jenks Mar 2012

Causes Of Pneumonia Epizootics Among Bighorn Sheep, Western United States, 2008–2010, Thomas E. Besser, Margaret A. Highland, Katherine Baker, E. Frances Cassirer, Neil J. Anderson, Jennifer M. Ramsey, Kristen Mansfield, Darren L. Bruning, Peregrine Wolff, Joshua B. Smith, Jonathan A. Jenks

Natural Resource Management Faculty Publications

Epizootic pneumonia of bighorn sheep is a devastating disease of uncertain etiology. To help clarify the etiology, we used culture and culture-independent methods to compare the prevalence of the bacterial respiratory pathogens Mannheimia haemolytica, Bibersteinia trehalosi, Pasteurella multocida, and Mycoplasma ovipneumoniae in lung tissue from 44 bighorn sheep from herds affected by 8 outbreaks in the western United States. M. ovipneumoniae, the only agent detected at signifi cantly higher prevalence in animals from outbreaks (95%) than in animals from unaffected healthy populations (0%), was the most consistently detected agent and the only agent that exhibited single strain types within each …


Spatial Ecology Of White-Tailed Deer Fawns In The Northern Great Plains: Implications Of Loss Of Conservation Reserve Program Grasslands, Troy W. Grovenburg, Robert W. Klaver, Jonathan A. Jenks Mar 2012

Spatial Ecology Of White-Tailed Deer Fawns In The Northern Great Plains: Implications Of Loss Of Conservation Reserve Program Grasslands, Troy W. Grovenburg, Robert W. Klaver, Jonathan A. Jenks

Natural Resource Management Faculty Publications

Few studies have evaluated how wildlife, and white-tailed deer (Odocoileus virginianus) in particular, respond to Conservation Reserve Program (CRP) grasslands. We conducted a 3-year study (2007– 2009) to determine the influence of CRP on fawn ecology during a time of declining CRP enrollment. We captured and radiocollared 81 fawn white-tailed deer during 15 May to 15 June 2007–2009 in north-central South Dakota, collected 6,505 locations, and documented 70 summer home ranges. Mean summer home ranges increased temporally during 2007–2009 (P < 0.001) and corresponded to a 41% loss of CRP grasslands in the area (2.3% loss in land cover and approx. 21% loss in cover habitat in the study area) over the duration of the study. Additionally, mean movement between daily locations increased (P < 0.001) from 2007 to 2009. Analysis of covariance models indicated that change in CRP influenced home-range size, and change in CRP and wheat influenced daily movement. Smaller home ranges and reduced movements were associated with greater quantity of CRP available to fawns, and increased movements were associated with more acreage of wheat available to fawns. Fawns shifted resource selection during the summer at a mean age ranging from 48.8 days to 58.6 days, and this shift was associated with height of corn (83–87 cm). During early summer, fawns consistently selected for CRP; selection of wheat progressed temporally from avoidance in 2007 to selection in 2009. During late summer, fawns consistently selected for corn habitat and used CRP at least in proportion to its availability. Reduction in CRP-grasslands seemed to increase fawn home-range size and daily movements and, influenced change in resource selection to wheat. Current legislation mandates continued decrease in CRP enrollment and concomitant increase in the planting of corn for ethanol production. Management of habitat throughout the grasslands of the Northern Great Plains that maximizes cover habitats would provide neonates with adequate cover for protection from predators.


News Of The Department Of Animal And Range Sciences, Department Of Animal And Range Sciences Feb 2012

News Of The Department Of Animal And Range Sciences, Department Of Animal And Range Sciences

Animal Science Newsletters

No abstract provided.


Hierarchy In Factors Affecting Fish Biodiversity In Floodplain Lakes Of The Mississippi Alluvial Valley, Daniel J. Dembkowski, L. E. Miranda Feb 2012

Hierarchy In Factors Affecting Fish Biodiversity In Floodplain Lakes Of The Mississippi Alluvial Valley, Daniel J. Dembkowski, L. E. Miranda

Natural Resource Management Faculty Publications

River-floodplain ecosystems offer some of the most diverse and dynamic environments in the world. Accordingly, floodplain habitats harbor diverse fish assemblages. Fish biodiversity in floodplain lakes may be influenced by multiple variables operating on disparate scales, and these variables may exhibit a hierarchical organization depending on whether one variable governs another. In this study, we examined the interaction between primary variables descriptive of floodplain lake large-scale features, suites of secondary variables descriptive of water quality and primary productivity, and a set of tertiary variables descriptive of fish biodiversity across a range of floodplain lakes in the Mississippi Alluvial Valley of …


Cottonwood And Antelope Range And Livestock Research Stations Unit Report, Daniel B. Oedekoven Jan 2012

Cottonwood And Antelope Range And Livestock Research Stations Unit Report, Daniel B. Oedekoven

South Dakota Beef Report, 2012

The Cottonwood and Antelope Range and Livestock Research Stations, located in western South Dakota, are used for research projects focused on the needs of range livestock producers in that region. The stations are comprised primarily of native rangeland that is grazed by cattle at both stations, and also by sheep at Antelope. The philosophy of the research efforts has been focused on conducting applied research to solve problems and address rangeland and livestock management opportunities relevant to the livestock producers and land managers of the region.


Sdsu Cow/Calf Teaching And Research Unit, C. Wright, K. Vander Wal, G. Perry Jan 2012

Sdsu Cow/Calf Teaching And Research Unit, C. Wright, K. Vander Wal, G. Perry

South Dakota Beef Report, 2012

The SDSU Cow/Calf Unit (CCU) is a multi-purpose facility that provides resources for Animal Science courses and research projects. Cattle produced at the facility are also utilized by Little International, Block and Bridle, and livestock judging teams. The facility is managed by Kevin Vander Wal and generally employs 4 to 5 undergraduate students.


The Influence Of Caspase-3 On The Calpain Enzyme System During Meat Aging, D.A. Mohrhouse, S.A. Kern, K.R. Underwood, A.D. Weaver Jan 2012

The Influence Of Caspase-3 On The Calpain Enzyme System During Meat Aging, D.A. Mohrhouse, S.A. Kern, K.R. Underwood, A.D. Weaver

South Dakota Beef Report, 2012

Tenderness is a key component of palatability, which influences consumers’ perception of meat quality. There are a variety of factors that contribute to variations in tenderness, including postmortem proteolysis. A more complete understanding of this biological mechanism regulating tenderness is needed to ensure consistently tender beef. Numerous reports indicate μ-calpain is primarily responsible for the degradation of proteins postmortem. Additionally, it has been shown that caspase-3 can cleave calpastatin, the inhibitor of μ-calpain. Therefore, the objective of this study was to determine if in vitro degradation of calpastatin by caspase-3 can enhance the postmortem breakdown of myofibrillar proteins by μ-calpain. …


Sdsu Calf Value Discovery 2011 Summary Report, J.A. Walker, B.P. Holland, C.L. Wright, W.C. Rusche Jan 2012

Sdsu Calf Value Discovery 2011 Summary Report, J.A. Walker, B.P. Holland, C.L. Wright, W.C. Rusche

South Dakota Beef Report, 2012

The Calf Value Discovery Program (CVD) allows cow-calf producers to gain knowledge of the finishing segment of the beef cattle industry and the marketing of fed cattle. Specifically, CVD provides an opportunity for cow-calf producers to learn how their calves perform in a feedlot and their carcass value when sold in a value-based marketing system. Each producer taking part in the program could consign a minimum of 5 steers weighing between 500 and 800 pounds to the CVD program. Animals were finished in a calf-fed program using typical diets and management protocols at VanderWal Yards (Bruce, SD). Carcass and feedlot …


Cost Analysis Of Cattle Feedlot Designs, J.M. Mrozinski, R.H. Pritchard, B.P. Holland, G.W. Warmann Jan 2012

Cost Analysis Of Cattle Feedlot Designs, J.M. Mrozinski, R.H. Pritchard, B.P. Holland, G.W. Warmann

South Dakota Beef Report, 2012

A cost analysis of fixed and non-fixed costs of gain was conducted on 3 cattle feedlot designs. The three facility designs compared were conventional open pens (OPN), open pens with shelter over the feeding area (OS), and a monoslope confinement barn (MON). The OPN design was the least expensive facility to build and operate. However, because of poorer cattle performance (P < 0.05), it was not the most cost effective. The MON design had significantly higher operating costs when compared to the OPN or OS designs, especially for the tractor/spreader (P < 0.05), skid loader (P < 0.10), labor (P < 0.05), and straw (P < 0.05). The operating and fixed costs, combined, made the MON design the most expensive cost of gain design (P < 0.05). The OS design was the most expensive facility to build but with current feed prices and the cattle performance, it was the most cost effective cattle feeding design.


Relationship Between Fat Content And Ne Values For Some Ethanol Byproducts, R. Pritchard, E. Loe, T. Milton Jan 2012

Relationship Between Fat Content And Ne Values For Some Ethanol Byproducts, R. Pritchard, E. Loe, T. Milton

South Dakota Beef Report, 2012

A finishing phase study was conducted to measure the impact of the fat content of ethanol byproducts on the relative Net Energy values for these feedstuffs. The three feedstuffs with varying crude fat content used included a commercially available corn gluten feed corn distillers grains blend (CGD); a corn wet distillers grains without solubles (WDG); and corn wet distillers grains plus solubles (WDGS). Byproducts were incorporated as 40% of the finishing diets (DM basis) replacing corn and SBM components of the control (CO) diet. There were 6 pens of 7 or 8 yearling steers on each treatment during the 130 …


Comparing Pfizer Genestar And Igenity Profile Dna Tests In Crossbred Cattle, M.G. Gonda, G.A. Perry, B.P. Holland, C.L. Wright Jan 2012

Comparing Pfizer Genestar And Igenity Profile Dna Tests In Crossbred Cattle, M.G. Gonda, G.A. Perry, B.P. Holland, C.L. Wright

South Dakota Beef Report, 2012

Our objective was to compare the performance of Pfizer’s GeneSTAR and Igenity’s PROFILE DNA tests in crossbred beef cattle. Hair follicles were collected from 394 crossbred steers that were part of the South Dakota State University Calf Value Discovery project (n = 145) and calves that were fed at the SDSU Southeast Research Farm (n = 249) in 2011. Hair follicles were sent to Pfizer Animal Genetics and Igenity for testing with their GeneSTAR and PROFILE DNA tests, respectively. Marbling score (100-999 scale), ribeye area, fat thickness, carcass weight, yield grade, quality grade, and % kidney, pelvic, and heart fat …


Effects Of Weaning Age And Winter Development Environment On Heifer Grazing Distribution, N.L. Hojer, M.B. Hubert, P.S. Johnson, M.H. Price Jan 2012

Effects Of Weaning Age And Winter Development Environment On Heifer Grazing Distribution, N.L. Hojer, M.B. Hubert, P.S. Johnson, M.H. Price

South Dakota Beef Report, 2012

The objective of this experiment was to determine if early weaning (approximately 125 d) vs. normal weaning (approximately 250 d) and wintering replacement heifers in drylot versus rangeland affected heifer grazing distribution during the subsequent summer. Heifer calves from the 2009 and 2010 calf crop (n = 104 and 73, respectively) were allocated to the 2 weaning treatments and then stratified by age into the 2 winter development treatments. During the summer of yr 1 heifers were allocated to 2 pastures by winter treatment, and in yr 2, all 4 treatment combinations were allocated to separate pastures. A subset of …


Influence Of Propionate Salt Levels On Young Cow Reproductive Performance, J.A. Walker, G.A. Perry, K.C. Olson Jan 2012

Influence Of Propionate Salt Levels On Young Cow Reproductive Performance, J.A. Walker, G.A. Perry, K.C. Olson

South Dakota Beef Report, 2012

A supplementation study was conducted to evaluate level of propionate salt (Ca-propionate) on young cow performance over two years. One hundred-twenty cows were allocated to one of three treatments at calving. Propionate salt was incorporated in a protein supplement at a rate of 0, 80 or 160 g/d. Cows were individually supplemented twice weekly at 2 lbs/d. In year 1, cows had access to pasture and hay. In year 2, cows had access to a native range pasture. Blood was collected weekly and analyzed for progesterone (P4) to determine postpartum interval (≥1 ng P4/ml). Weights and body condition scores (BCS) …


The Jackrabbit Dairy Review, Vikram Mistry Jan 2012

The Jackrabbit Dairy Review, Vikram Mistry

The Jackrabbit Dairy & Food Review

No abstract provided.


Comparison Of Three Cidr Based Fixed-Time Ai Protocols For Beef Heifers, G.A. Perry, J.K. Grant, J.A. Walker, G.A. Bridges, S.G. Kruse, S. Bird, K. Heaton, R. Arias, S.L. Lake Jan 2012

Comparison Of Three Cidr Based Fixed-Time Ai Protocols For Beef Heifers, G.A. Perry, J.K. Grant, J.A. Walker, G.A. Bridges, S.G. Kruse, S. Bird, K. Heaton, R. Arias, S.L. Lake

South Dakota Beef Report, 2012

Several effective fixed-time AI (FTAI) protocols have been developed to facilitate AI while eliminating the need for estrus detection. Among these are the 5-d CO-Synch+CIDR (5d), PG 6-d CIDR (PG-CIDR), and 14-d CIDR-PG (CIDR-PG) protocols. While each of these protocols varies in duration and approach to synchronizing estrus and ovulation, each has been reported as an effective method to facilitate FTAI in beef heifers. Therefore, the objective of this study was to compare FTAI pregnancy rates in beef heifers synchronized with these three CIDR based protocols. Virgin beef heifers (n = 801) at four locations were synchronized with one of …


Effects Of Weaning Age And Winter Development Environment On Heifer Performance, N.L. Hojer, M.B. Hubert, P.S. Johnson, M.H. Price, K.C. Olson Jan 2012

Effects Of Weaning Age And Winter Development Environment On Heifer Performance, N.L. Hojer, M.B. Hubert, P.S. Johnson, M.H. Price, K.C. Olson

South Dakota Beef Report, 2012

Our objective was to determine if early weaning (about 125 d) vs. normal weaning (about 250 d) and wintering replacement heifers in drylot vs. rangeland affected heifer growth and reproductive performance. Heifer calves from the 2009 and 2010 calf crops (n = 104 and 73, respectively) were allocated to the 2 weaning treatments and then stratified by age into the 2 winter development treatments forming a 2 by 2 factorial arrangement of treatments. Heifers wintered in drylot received mixed grass and alfalfa hay (yr 1: 11.6% CP, 52.5% TDN; yr2: 12.3% CP, 53.4% TDN) plus 1.8 kg of a dried …


Investigation Of Protease Activity In Early Postmortem Musclesubjected To Alternative Chilling Conditions, D.A. Mohrhouse, S.M. Lonergran, E. Huff-Lonergran, K.R. Underwood, A.D. Weaver Jan 2012

Investigation Of Protease Activity In Early Postmortem Musclesubjected To Alternative Chilling Conditions, D.A. Mohrhouse, S.M. Lonergran, E. Huff-Lonergran, K.R. Underwood, A.D. Weaver

South Dakota Beef Report, 2012

The objective of this study was two-fold: 1) determine the conditions (temperature, pH) that exist in early postmortem muscle of normally-chilled and delay-chilled beef carcasses to provide a model for in vitro work, and 2) determine the mechanism by which specific early postmortem temperature/pH conditions found in normally-chilled and delay-chilled muscle influence the enzymes that regulate the aging process in vitro. For objective 1, seven market-ready calves (HCW 848 ± 17.5 lb) were harvested with the right sides subjected to normal chilling (2.3°C) and the left sides subjected to ambient temperature (delay chilling; 22.6°C) for an additional 4.75 h and …