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Full-Text Articles in Animal Sciences
Population Genomic Transformations Induced By Isolation Of Wild Bird Avian Influenza Viruses (Orthomyxoviridae) In Embryonated Chicken Eggs, Matthew W. Hopken, Antoinette J. Piaggio, K. L. Pabilonia, James Pierce, Theodore Anderson, Courtney Pierce, Zaid Abdo
Population Genomic Transformations Induced By Isolation Of Wild Bird Avian Influenza Viruses (Orthomyxoviridae) In Embryonated Chicken Eggs, Matthew W. Hopken, Antoinette J. Piaggio, K. L. Pabilonia, James Pierce, Theodore Anderson, Courtney Pierce, Zaid Abdo
USDA Wildlife Services: Staff Publications
Isolation and cultivation of wild-type viruses in model organism cells or tissues is standard practice in virology. Oftentimes, the virus host species is distantly related to the species from which the culture system was developed. Thus, virus culture in these tissues and cells basically constitutes a host jump, which can lead to genomic changes through genetic drift and/or adaptation to the culture system. We directly sequenced 70 avian influenza virus (Orthomyxoviridae) genomes from oropharyngeal/cloacal swabs collected from wild bird species and paired virus isolates propagated from the same samples following isolation in specific-pathogen-free embryonated chicken eggs. The data were analyzed …