Animal Sciences Commons^™

Effects Of Spirulina And Nannochloropsis Supplementations On The Meat Quality And Sensory Properties Of Broiler Carcasses, Shreyash S. Patel

Master's Theses

Microalgae are a promising feed ingredient due to their high content of proteins, lipids, polysaccharides, vitamins, pigments, and enzymes. This study evaluated the effects of algae as a dietary supplementation on the appearance, quality, and sensory attributes of broiler carcasses. In each of experiment I and II, a total of 54 Ross-708 broilers were randomly obtained from the pool of 270 broilers in the 18 floor pens of soybean-mealbased diets at 0, 2.5%, and 5% Spirulina (Experiment I) and Nannochloropsis (Experiment II) supplements in the Poultry Unit at California Polytechnic State University (Cal Poly, San Luis Obispo, CA). Broiler carcasses …

An Unusual Trumpeter Swan (Cygnus Buccinator) In Nebraska: Albinism Or Leucism?, Jacob C. Cooper, Peggy M. Huss, Eugene A. Huryn, Cody Mcgregor

Nebraska Bird Review

On 26 January 2025, we observed a large mixed flock of Trumpeter Swans (Cygnus buccinator) and Tundra Swans (C. columbianus) at Hansen Reservoir ca. 7 miles (11 km) NNW of North Platte along the east side of Nebraska Highway 97. The flock of 158 swans consisted of 4 “Whistling” Tundra Swans (C. c. columbianus), 153 typical Trumpeter Swans, and one individual that we were not immediately able to identify. Our unknown swan was in the company of a family group of Trumpeter Swans but immediately stood out due to its broad yellow base of …

Nebraska Ornithologists' Union Tri-State Meeting With Iowa Ornithologists' Union And South Dakota Ornithologists' Union, South Sioux City, Nebraska, May 16-18, 2025

Nebraska Bird Review

The NOU Spring Meeting was held at the Salvation Army Western Plains Camp in South Sioux City, Nebraska, in conjunction with the IOU and SDOU, with approximately 180 attending. The organizers of the meeting included Chris Anderson and Mark Schmidtbauer from South Dakota, Tyler Harms and Kevin Murphy from Iowa, and Ruth Stearns, Joel Jorgenson, and Paul Pearson from Nebraska.

A scientific poster session featuring the work of 9 ornithological researchers from UN-Kearney, USD-Vermillion, and Hitchcock Nature Center was held Friday evening. Thanks to Dr. Jacob Cooper from UNK, Dr. David Swanson from USD, and the students who participated.

Field …

Nou Tri-State Meeting—South Sioux City [Spring 2025 Nou Meeting Species Tally]

Nebraska Bird Review

Bird species tally (185) for the Spring 2025 NOU Tri-State Meeting, South Sioux City (Nebraska Ornithologists’ Union Tri-State Meeting with Iowa Ornithologists’ Union and South Dakota Ornithologists’ Union, South Sioux City, Nebraska, May 16–18, 2025)

Abstracts Of Posters Presented At South Sioux City Nou Meeting

Nebraska Bird Review

A scientific poster session featuring the work of 9 ornithological researchers from UN-Kearney, USD-Vermillion and Hitchcock Nature Center was held Friday evening. Thanks to Dr. Jacob Cooper from UNK, Dr. David Swanson from USD, and the students who participated.

Wandering from the River: On- and Off-River Abundance of Interior Least Terns along the 59-Mile Reach of the Missouri National Recreational River. Zoe Donelan, Hannah Slattery, Kyle Jorgensen, and David L. Swanson, University of South Dakota, Department of Biology.

Preliminary Results of Raptor Migration Monitoring at Hitchcock Nature Center. Kellie Hayden, Jerry Toll, and Sandy Reinken, Hitchcock Nature Center, Pottawattamie Conservation, …

Spring Field Report, March–May 2025, W. Ross Silcock

Nebraska Bird Review

Perhaps the most important takeaway this spring is the continuing phenomenon of large numbers of species with early spring arrival dates. Although about half as many as 2024, there were 64 examples of either tied or new early arrival dates, most obviously of insectivores such as swifts, flycatchers, vireos, swallows, pipits, longspurs, and 10 warbler species. Among these, record or near-record early were Whip-poor-will, Black-necked Stilt, Marbled Godwit, Black Tern, Olive-sided Flycatcher, Purple Martin, Rock Wren, Gray Catbird in central, Spotted Towhee in north and west, and Hooded Warbler.

Following along logically perhaps with early arrival dates were a number …

The Nebraska Bird Review (June 2025) 93(2), Whole Issue

Nebraska Bird Review

Spring Field Report, March–May 2025, by W. Ross Silcock

NOU Spring Field Days and Annual Meeting in South Sioux City, May 16–18, 2025

Abstracts of posters presented at Spring 2025 NOU Meeting

Spring 2025 NOU Meeting Species Tally

An Unusual Trumpeter Swan (Cygnus buccinator) in Nebraska: Albinism or Leucism? by Jacob C. Cooper, Peggy M. Huss, Eugene A. Huryn, and Cody McGregor

Subscription and Organization Information

Re-Evaluating The Host Specificity Of Thirteen Eimeria Species That Infect Galliform Birds: Cross-Infection Experiments In Chukar Partridges (Alectoris Chukar), Northern Bobwhites (Colinus Virginianus) And Turkeys (Meleagris Gallopavo), Jessica L. Rotolo, A. Leveille, Rachel K. Imai, Elizabeth G. Zeldenrust, Billy M. Hargis, Elijah G. Kiarie, John R. Barta

Poultry Science Faculty Publications and Presentations

The commercial poultry industry has expanded and profited from intensively rearing birds, but it is not without consequences. Coccidiosis is an intestinal disease caused by Eimeria species that is well recognized by poultry producers because of the resulting health implications that often contribute to reduced welfare of birds, meat production and profitability. Strict host specificity of these parasites (infection of one host species only) is often assumed; however, examples of Eimeria spp. capable of infecting multiple galliform hosts have been described. Despite the relevance of coccidiosis in commercial poultry production, the number and extent of such crossinfections remain poorly understood, …

The Effects Of Sirulina And Nannochloropsis Supplementation On Broiler Performance, Intestinal Morphology, And Gut Microbiology, Emily Beckerman

Master's Theses

The poultry industry is constantly searching for new nutritional strategies and better feed conversion ratio to enhance broiler growth and productivity. Microalgae have been shown to improve broiler performance and health depending on species and supplementation levels. The objective of this study was to determine the effects of Spirulina supplementation (Experiment I) and Nannochloropsis supplementation (Experiment II) on broiler production performance, intestinal morphology, and gut microbiology. In Experiment I, a total of 270 broiler chicks (one-day-old Ross 708) were obtained from a local hatchery after vaccinating against Newcastle, Marek’s disease, and Infectious Bronchitis. They were weighed and sorted into 18 …

One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika

All Peer-Reviewed Publications

The global human population is rapidly increasing, escalating interactions of people, animals and the environment. This has led to more convoluted health challenges, for which African smallholder farmers bear the brunt. The One Health (OH) approach recognises the interconnectedness of these health challenges and thus follows a transdisciplinary approach involving diverse stakeholders to address them. Although there have recently been global concerted efforts and studies on OH, an information gap exists in Africa, particularly regarding smallholder farmers and their needs. Additionally, evaluation frameworks and outcome metrics for OH initiatives are still unclear. Thus, this study was conducted to critically map …

Genetic Factors Of Campylobacter Jejuni Required For Its Interactions With Free-Living Amoeba, Deepti Pranay Samarth, Asim Z. Abbasi, Young Min Kwon

Poultry Science Faculty Publications and Presentations

Acanthamoeba, a free-living amoeba ubiquitous in environmental water, has been considered as the environmental reservoir of certain bacterial pathogens, including Campylobacter jejuni, an intracellular human pathogen causing self-limiting gastroenteritis. Acanthamoeba-C. jejuni interaction mechanisms may help clarify how the otherwise fastidious bacterium C. jejuni survives in environmental waters. In this study, we constructed single deletion mutants of C. jejuni strain 81-176 for the 10 selected genes (motAB, ciaB, kpsE, virB11, cheY, flaAB, cstII, docB, sodB, and cadF) previously shown to be important for the interaction (invasion and intracellular survival) of C. jejuni with mammalian hosts. We used a modified gentamicin protection …

The Effects Of Incubation Humidity On Broiler Chicks, Claire Caples

Animal Science Undergraduate Honors Theses

Leg health issues are a welfare problem in commercial broiler chickens and an economic issue for the broiler industry. Broilers exhibiting leg abnormalities show altered gait, reduced ability to access resources, and increased lameness incidence. These leg health issues can result from many animal and environmental factors during incubation and post-hatch. Incubation strategies may contribute to the development of leg health issues, and recent research has increased focus on incubation factors affecting embryonic development and health and welfare post-hatch. Thus, the objective of this study was to evaluate the effect of high (80%), low (55%), and ideal (65%) humidity on …

Developing Protective Measures To Reduce The Incidence Of Bacterial-Induced Chondronecrosis With Osteomyelitis In Broiler Chickens, Alexa L. Thomas

Animal Science Undergraduate Honors Theses

Bacterial Chondronecrosis with Osteomyelitis (BCO) lameness is a significant animal welfare and food safety issue affecting the commercial broiler industry in the United States and worldwide. Bacteria can translocate from the respiratory system and/or the gastrointestinal tract into the bloodstream, eventually settling in bone microfractures leading to necrosis and BCO lameness.

This study evaluated the effects of Probiotic 1 and Probiotic 2 on the incidence of BCO lameness in broiler chickens. Probiotic 1 was applied as a spray on Day 1-old chicks, while Probiotic 2 was administered prophylactically (orally in feed) from Day 1 to Day 56. Birds were assigned …

Utilizing 16s Ribosomal Rna Sequencing To Identify Bacterial Species Colonizing Lame Birds With Bacterial Chondronecrosis With Osteomyelitis, Kara Hoover

Animal Science Undergraduate Honors Theses

Broiler chicken lameness has become prevalent in recent years due to high growth rates in broilerchickens. Bacterial chondronecrosis with osteomyelitis (BCO), the main cause of lameness, is characterized by necrosis of femoral and tibial heads in affected birds. Heavier birds are more susceptible to mechanical stress in these regions, which results in microfractures, leading to lameness. BCO lameness poses huge financial pressure to the poultry industry, as 14-50% of flocks can become affected during outbreaks. The purpose of this experiment was to identify bacterial species present in birds with BCO lameness. A total of 1,560 chicks were randomly distributed into …

The Effect Of 1,25-Dihydroxyvitamin D3 Supplementation On Humoral Immunity Against Bacterial Chondronecrosis With Osteomyelitis Lameness In Broiler Chickens, Bailey Herberger

Animal Science Undergraduate Honors Theses

Bacterial chondronecrosis with osteomyelitis (BCO) lameness, the most predominant locomotive illness in fast-growing broilers, causes substantial animal well-being issues and affects broiler production, leading to huge financial losses. Several strategies, including feed supplementations using organic trace minerals, vitamin D, probiotics, and prebiotics, have been utilized to alleviate BCO lameness incidences in broiler chickens. Previous studies have indicated that feeding an additive of Panbonis^®-G-1,25(OH)₂D₃ from Solanum glaucophyllum containing 1 µg/kg G-1,25(OH)₂D₃ to broilers for the first 28 days of their production cycle reduced BCO lameness by more than 50%. In this study, we …

Effects Of Intradermal Injections Of Lipopolysaccharide On The Local And Systemic Acute Inflammatory Responses In Turkeys, Delaney F. Roberts

Poultry Science Undergraduate Honors Theses

In chickens, the growing feather (GF) pulp has been established as a cutaneous bioassay to monitor and assess tissue/cellular immune activities in response to intradermal (i.d.) injections of test materials. The objective of this pilot study was to determine the suitability of the GF bioassay in turkeys by examining the acute inflammatory responses initiated by i.d. GF-pulp injections of lipopolysaccharide (LPS). For this, three, 5-wk-old turkey poults were used. Two poults had 8 GFs injected (10 μL/GF) with low (L)-LPS (0.1 μg/GF) and 8 GFs with high (H)-LPS (1 μg/GF), while a third poult had 8 GFs injected with endotoxin-free …

Inflammatory Response In Ucd200/206 Scleroderma-Prone Chickens To Intradermal Injection Of Silikon 1000, Katee L. Johnson

Animal Science Undergraduate Honors Theses

Intradermal injections of silicone materials have resulted in chronic inflammation and fibrotic reactions in some individuals. The UCD200/206 line of chickens is characterized by spontaneous development of scleroderma/systemic sclerosis (SSc), a fibrotic autoimmune disease. This longitudinal study examined the cellular inflammatory activity initiated by SILIKON 1000 (a silicone product used as a filler in human skin) at the site of intradermal (i.d.) injection and in the peripheral blood in the SSc-prone UCD 200/206 (UCD) chickens and healthy White Leghorn (WL) controls. Specifically, the pulps of growing feathers (GFs) were i.d. injected with SILIKON or endotoxin-free, sterile PBS (8 and 3 …

The Effect Of 1,25-Dihydroxyvitamin D3 Supplementation On Humoral Immunity Against Bacterial Chondronecrosis With Osteomyelitis Lameness In Broiler Chickens, Bailey Herberger

Animal Science Undergraduate Honors Theses

Bacterial chondronecrosis with osteomyelitis (BCO) lameness, the most predominant locomotive illness in fast-growing broilers, causes substantial animal well-being issues and affects broiler production, leading to huge financial losses. Several strategies, including feed supplementations using organic trace minerals, vitamin D, probiotics, and prebiotics, have been utilized to alleviate BCO lameness incidences in broiler chickens. Previous studies have indicated that feeding an additive of Panbonis^®-G-1,25(OH)₂D₃ from Solanum glaucophyllum containing 1 µg/kg G-1,25(OH)₂D₃ to broilers for the first 28 days of their production cycle reduced BCO lameness by more than 50%. In this study, we …

Evaluation Of Fecal Egg Counts (Fec) As A Viable Means To Estimate Ascaridia Galli, Heterakis Gallinarum And Capillaria Obsignata Burdens In Laying Hens, Alexander H. Moss

Animal Science Undergraduate Honors Theses

The control of gastrointestinal helminths, specifically Ascaridia galli, Heterakis gallinarum, and Capillaria obsignata, is a critical component of poultry health management, particularly in free-range and organic farming systems. Methods of estimating parasite burdens, such as post-mortem examinations, are not feasible for large-scale commercial operations due to their invasiveness and time requirements. This study aimed to evaluate the use of fecal egg counts (FEC) as a non-invasive and efficient method for estimating the worm burdens of these parasites in laying hens. A flock of 12 brown egg laying, spent, hens were individually penned on concrete. Fecal samples (both cecal and intestinal) …

The Expression Of Intestinal Tight Junction Proteins Induced By 1,25-Dihydroxyvitamin D3-Glycosides Supplementation To Reduce Bacterial Chondronecrosis With Osteomyelitis Lameness In Broilers, Kennedy M. Hicks

Biological Sciences Undergraduate Honors Theses

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a leading cause of lameness in broiler chickens, impacting animal welfare and commercial poultry production. Supplementation using organic trace minerals, vitamin D, probiotics, and prebiotics has been studied to mitigate BCO lameness incidences in broiler chickens. Our previous study demonstrated that feeding Panbonis®-G-1,25(OH)₂D₃ from Solanum glaucophyllum, containing 1 µg/kg G-1,25(OH)₂D₃, to broilers for the first 28 days of their production cycle reduced BCO lameness by approximately 50%. In this study, we investigated the impact of 1,25-dihydroxy vitamin D₃ supplementation on the expression of tight junction …

A Comparative Study Of Urban And Natural Nesting Gray Hawks In The Lower Rio Grande Valley Of South Texas, Evan Farese

Theses and Dissertations

Urbanization can negatively impact birds of prey, which occupy higher trophic levels. The Gray Hawk (Buteo plagiatus) is a neotropical raptor inhabiting the Lower Rio Grande Valley (LRGV) of Texas, where it is threatened. The species’ ecology has been little studied and it is unclear how urbanization may affect population viability. I studied Gray Hawk reproductive success, diet, and thermal ecology at nests in urban and natural habitats in the LRGV. Camera trap data showed adults at urban nests delivered mostly avian prey, while mammalian prey was most frequently brought to natural nests. Data from temperature loggers showed …

Sustainable Poultry Farming: A Concept Of Iot-Based Poultry Management System For Small-Scale Farmers, Ayodeji Akinsoji Okubanjo, Ignatius Kema Okakwu, Olufemi Peter Alao, Nurudeen Samuel. Lawal, Ayoola Abiola Babalola, Abisola Olayiwola

Al-Bahir

Conventional poultry management techniques are failing to meet increased demand for poultry products as the population continues to grow. As a result, this issue has become a major concern for small-scale farmers, particularly those in low-income areas, in terms of food security. One of the main reasons for this is that the farmers rely on intensive farming methods which are inefficient for automating daily poultry operations. However, intensive farming methods pose major environmental concerns to ecosystems and poultry health. Also, the environmental conditions, welfare, and productivity of poultry operations may be harmed by the global climate crisis and poultry waste …

Mdpk Cell Lines Can Be Used For In Vitro Antiviral Testing With Highly Pathogenic Avian Influenza (H5n1), Jada L. Westover

Student Research Symposium

• Outbreak in agricultural poultry starting in 2022
• In March 2024 first confirmed case in Dairy Cattle
• Tested drugs T705, Oseltamivir, Baloxivir, Ribavirin, EIDD-2947, and EIDD-2749 on three different cell lines
• Goal: See if MDBK cell lines could be used for in vitro studies

Effect Of Heat Stress On Ileal Epithelial Barrier Integrity In Broilers Divergently Selected For High- And Low-Water Efficiency, Elizabeth S. Greene, Brooklee Roach, Maria Fernandez Cuadrado, Sara Orlowski, Sami Dridi

Poultry Science Faculty Publications and Presentations

Water scarcity and rising global temperatures are two of the greatest current and future threats to poultry sustainability. Therefore, selection for water efficiency (WE) and heat resilience are of vital importance. Additionally, intestinal integrity is of critical importance under challenging conditions to maintain nutrient absorption and therefore, growth and performance of broilers. Here, we examined the effect of chronic cyclic heat stress (HS) on the ileal expression profile of tight-junction, gap-junction, adherens, and desmosome genes in the fourth generation of divergently selected low (LWE)- and high water efficient (HWE)-chicken lines. LWE birds exhibited higher levels of gut permeability, regardless of …

Evaluation Of The Effect Of In Ovo Applied Bifidobacteria And Lactic Acid Bacteria On Enteric Colonization By Hatchery-Associated Opportunistic Pathogens And Early Performance In Broiler Chickens, Mitchell C. Rowland, Aaron J. Forga, James Higuita, Makenly E. Coles, Billy M. Hargis, Christine N. Vuong, Danielle Graham

Poultry Science Faculty Publications and Presentations

Probiotics have been used to promote pioneer colonization by beneficial bacteria in poultry. The purpose of the present study (four trials) was to determine if an in ovo injection with Bifidobacterium saeculare (B2-2, B3-4) and/or lactic acid bacteria (LAB18, LAB46) at d18 of embryogenesis reduced Enterococcus and Gram-negative bacterial colonization in the gut at hatch. An environmental challenge model was used to simulate microbial contamination in large-scale hatch cabinets in a laboratory setting. In all trials, gut homogenates obtained from chicks at hatch were plated to enumerate relevant bacterial populations. Performance was evaluated in trials two and four. The top …

The Mechanistic Target Of Rapamycin Mediates Clostridium Perfringens-Induced Chicken Necrotic Enteritis Attenuated By Secondary Bile Acid Deoxycholic Acid, Mohit Bansal, Tahrir Alenezi, Ying Fu, Janashrit Shrestha, Ayidh Almansour, Hong Wang, Anamika Gupta, Rohana Liyanage, Xiaolun Sun

Poultry Science Faculty Publications and Presentations

Clostridium perfringens is a prevalent gut bacterial pathogen in humans and animals. This study investigated the role of the mechanistic targets of rapamycin (mTOR) and deoxycholic acid (DCA) on C. perfringens intestinal infection. Chickens were sequentially infected with Eimeria maxima and received the mTOR inhibitor rapamycin and DCA. C. perfringens-induced necrotic enteritis (NE) was evaluated using body weight gain (BWG), histopathology, bile acids, pathogen colonization, cell infiltration and death, and gene expression. The significant difference of p < 0.05 was analyzed by one-way ANOVA and multiple comparisons. Notably, rapamycin strongly reduced the subclinical and clinical NE histopathologies. DCA and DCA combined with rapamycin alleviated clinical NE and BWG loss. Rapamycin, DCA, and DCA + rapamycin attenuated bile acid reduction in NE birds, and they also reduced immune cell infiltration into the intestinal lamina propria as well as immune cell migration in vitro. At molecular levels, DCA and DCA + rapamycin reduced proinflammatory IFN gamma, MMP9, IL23, and IL17 gene expression. Rapamycin, DCA, and DCA + rapamycin reduced NE-induced intestinal cell apoptosis. Together, these results suggest that mTOR signaling mediates C. perfringens-induced ileitis, and combining mTOR inhibition and DCA improves the intervention efficacy against NE ileitis and BWG loss.

Hatching And Caring For Chicks, Cory Farnsworth, Jacob Hadfield, Ashley Longmore

All Current Publications

There are many reasons people are interested in raising birds. Most people who desire to start are interested in saving money and becoming more self-sufficient. Many people find excitement in hatching eggs and raising chicks. This fact sheet will help you get started with hatching eggs and raising chicks.

Empowering Young Innovators Through School-Based Agricultural Education: Insights From A Nigerian Secondary School Teacher, Mary O. Olumide-Oyaniyi

National Collaborative for Research on Food, Energy, and Water Education (NC-FEW)

Nigeria faces challenges like food insecurity and economic instability, worsened by a growing youth population. School-Based Agricultural Education (SBAE) engages youth in agricultural innovations. This study implemented SBAE principles in a Nigerian school, combining poultry farming and aquaculture. Students managed a poultry farm, using chicken droppings to breed maggots as feed for catfish in rainwater-filled tanks. The project enhanced students' skills, improved local food production, and engaged the community. Despite funding and management challenges, the initiative demonstrated SBAE's potential to empower youth and advance agricultural practices, offering a model for similar projects in other regions.

The Nebraska Bird Review (March 2025) 93(1), Whole Issue

Nebraska Bird Review

Winter Field Report, December 2024–February 2025, by W. Ross Silcock
2024–2025 Christmas Bird Counts, by Don Paseka
Annual Color Photo Section
Annual Treasurer’s Report for 2024, by Jan Johnson
Subscription and Organization Information

Annual Color Photo Section

Nebraska Bird Review

The following seven pages show examples of this year’s theme: birds feeding or carrying food.

Osprey with striped bass
Spotted Sandpiper with beetle
Scissor-tailed Flycatcher with insect
Male Pine Grosbeak with green ash seeds
Merlin eating sparrow
Whooping Crane carrying ear of corn
Yellow-billed Cuckoo with harvestman spider
Black-billed Magpie eating grain
Red-tailed Hawk with plains garter snake
Summer Tanager with hackberry emperor butterfly
Cattle Egret with bullfrog
Juvenile Red-shouldered Hawk eating garter snake
Red-breasted Merganser eating fish
Juvenile White Ibis eating unidentified item
Bohemian Waxwing eating crab apples
Rose-breasted Grosbeak eating sunflower seeds
Loggerhead Shrike eating dragonfly
Yellow-crowned Night …