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Articles 181 - 210 of 6112

Full-Text Articles in Animal Sciences

Overcoming Maternal Antibody Interference In Swine Influenza: Advancing Dna Vaccine Strateies For Enhanced Immune Protection In Piglets, Danh Cong Lai Jul 2025

Overcoming Maternal Antibody Interference In Swine Influenza: Advancing Dna Vaccine Strateies For Enhanced Immune Protection In Piglets, Danh Cong Lai

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Maternally derived antibodies (MDAs) play a vital role in protecting neonates from infectious diseases, but their presence at the time of vaccination can interfere with vaccine-induced immune responses, thereby reducing vaccine effectiveness. MDA interference has been documented in pigs vaccinated with whole inactivated virus (WIV) vaccines against swine influenza A virus (IAV). In this thesis, we evaluated the efficacy of a lipid nanoparticle encapsulated DNA (LNP-DNA) vaccine against swine IAV in the presence and absence of MDAs, comparing it to a WIV vaccine. In MDA-negative piglets, both the LNP-DNA and WIV vaccines induced strong immune responses and effectively prevented the …


Dietary Organic Selenium Supplementation Improved Some Gross Morphological Parameters And Biochemical Characteristics Of Testes In Young Male Kids, Nadia Rahim, Moolchand Malhi, Asma Babar, Muhammad Awais Soomro, Shazia Rahim Jun 2025

Dietary Organic Selenium Supplementation Improved Some Gross Morphological Parameters And Biochemical Characteristics Of Testes In Young Male Kids, Nadia Rahim, Moolchand Malhi, Asma Babar, Muhammad Awais Soomro, Shazia Rahim

Journal of Bioresource Management

Selenium (Se) plays a vital role in improving reproductive health in ruminants. This study evaluated the impact of Se supplementation on testicular health in young male goats. Twenty goats (2–3 months old, 8.5–10 kg) were randomly assigned to two groups: Control (C, n=10) receiving a basal diet (0.037 mg Se/kg) and Se-Yeast (SY, n=10) supplemented with 0.3 mg Se/kg diet. The trial lasted eight weeks. Results showed that testis weight was significantly higher in the SY group (26.8 ± 2.5 g) compared to C (22.4 ± 2.1 g, p < 0.05). Testis length, width, and circumference also increased significantly in SY goats (4.5 ± 0.3 cm, 2.9 ± 0.2 cm, and 7.4 ± 0.4 cm, respectively) compared to control (3.9 ± 0.4 cm, 2.5 ± 0.3 cm, and 6.7 ± 0.5 cm; p < 0.05). Serum cholesterol was higher in SY (135.27 ± 2.34 mg/dl) than in C (121.15 ± 8.74 mg/dl, p = 0.02), while triglycerides were lower (95.99 ± 1.37 vs. 114.38 ± 1.84 mg/dl, p = 0.0003). HDL increased (51.97 ± 2.10 vs. 45.02 ± 1.32 mg/dl, p = 0.03), and LDL decreased (54.23 ± 1.81 vs. 61.27 ± 1.56 mg/dl, p = 0.02) in SY group. Total serum protein rose significantly (8.14 ± 0.79 vs. 5.25 ± 0.06 mg/dl, p = 0.03), as did testosterone levels (1.95 ± 0.08 vs. 1.43 ± 0.06 ng/ml, p = 0.002). In conclusion, selenium supplementation significantly improved testicular morphology, lipid profile, protein levels, and testosterone, indicating enhanced reproductive health in goats.


The Factors Affecting The Distribution Of Scorpions (Arachnida: Scorpions) In Iran, Hassan Nasirian Jun 2025

The Factors Affecting The Distribution Of Scorpions (Arachnida: Scorpions) In Iran, Hassan Nasirian

Chulalongkorn Medical Journal

Background: The distribution of scorpions is influenced by various factors that determine their presence and abundance in different regions. Understanding these factors is crucial for comprehending scorpion ecology and predicting their potential impact on ecosystems. Objective: These subtopics aim to provide an overview of the distribution of scorpions within Iran and the factors affecting their distribution, highlighting their ecological preferences and adaptations to different regions.

Methods: Forty-nine documents were collected from well-known indexing services, including Elsevier, Google Scholar, PubMed, ScienceDirect, Scopus, Springer, and Web of Science. After carefully studying the documents, they were classified into specific topics and discussed.

Results: …


Nitrate And Nitrite Levels In Cattle Feed, Drinking Water, Rumen Content, And Blood Methemoglobin Levels In Burdur Province Of Türkiye, Reyda Kiyici, Hasan Altan Akkan, Mehmet Karaca, Esra Zeybek, Bayram Kudret Karaayvaz, Fi̇rdevs Mor Savaş, Yahya Öztürk, Asim Kart, Ramazan Yildiz, Murat Bayezi̇t Jun 2025

Nitrate And Nitrite Levels In Cattle Feed, Drinking Water, Rumen Content, And Blood Methemoglobin Levels In Burdur Province Of Türkiye, Reyda Kiyici, Hasan Altan Akkan, Mehmet Karaca, Esra Zeybek, Bayram Kudret Karaayvaz, Fi̇rdevs Mor Savaş, Yahya Öztürk, Asim Kart, Ramazan Yildiz, Murat Bayezi̇t

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the risk factors associated with nitrate/nitrite poisoning in cattle in the Burdur region. Feed and water samples were collected from 10 different farms randomly selected from 9 districts. A total of 33 feed samples, 13 water samples, 21 rumen contents, and 20 blood samples were collected for analysis. Nitrate and nitrite levels in feed, drinking water, and rumen fluid, as well as methemoglobin levels in blood samples, were determined using a spectrophotometric method. The average nitrate and nitrite levels in feed samples were 3.06 mg/kg and 3.54 mg/kg, respectively. The mean nitrate and nitrite …


Field Journal: The Excluded Middle, Carrie E. Kouts May 2025

Field Journal: The Excluded Middle, Carrie E. Kouts

Masters Theses

What does it look like to engage with organisms and landscapes at the periphery of anthropocentric value structures? How does one break the internalized myth that the “built” environment is excluded from the natural world? When does a hyper-mobile and hyper-commodified society confront the exponential crisis of animal death? Within this series of journal entries, field notes, collection observations, weird prose, and sensory musings, one will find questions on the nature of being human and the complex narratives of care we encounter in a world shared with more-than-humans. Each handwritten vignette and photograph from daily life weaves a non-linear and …


Exploring The Landscape Of Animal Advocacy In Egypt: Insights Into Stray, Wildlife, And Farmed Animal Rights, Yara Moussa, Wasseem Emam May 2025

Exploring The Landscape Of Animal Advocacy In Egypt: Insights Into Stray, Wildlife, And Farmed Animal Rights, Yara Moussa, Wasseem Emam

General - Animal Protection

This article examines the landscape of animal advocacy in Egypt, a nascent and growing field in one of Africa’s largest economies and most densely populated countries. It focuses on three types of animal advocacy: 1) stray animal welfare, 2) wildlife conservation, and 3) farmed animal welfare. The paper critically analyses current practices and trends in each of these areas, highlighting the main challenges and opportunities facing the growth of the movement. Our research draws on insights from interviews with active animal welfare organizations, situating their practical experiences, daily operational struggles, as well as instances of success, within Egypt’s wider socio-economic …


Biomarkers That Could Explain Parasite-Induced Behavioral Alterations In The Golden Shiner (Notemigonus Crysoleucas) – Posthodiplostomum System, Laura D. Nicolas May 2025

Biomarkers That Could Explain Parasite-Induced Behavioral Alterations In The Golden Shiner (Notemigonus Crysoleucas) – Posthodiplostomum System, Laura D. Nicolas

All HCAS Student Capstones, Theses, and Dissertations

Trophically transmitted parasites require an intermediate host to be consumed and often manipulate host phenotype (i.e., morphology, physiology, and/or behavior) to increase predation risk by definitive hosts. Posthodiplostomum is a trematode with a complex life cycle involving snails as first hosts, fish as second intermediate hosts, and piscivorous birds as definitive hosts. In fish hosts, Posthodiplostomum infection has been linked to increased predation, suggesting suppression of antipredator behaviors. However, the specific physiological pathways remained unexplored and changes in the host’s neural, endocrine, and immune systems could be involved. Thus, I examined whether Posthodiplostomum infection intensity was associated with brain serotonin …


Mechanisms Of Behavioral Change In Hosts Of Toxoplasma Gondii: A Review, Olivia Grimsley May 2025

Mechanisms Of Behavioral Change In Hosts Of Toxoplasma Gondii: A Review, Olivia Grimsley

Senior Honors Theses

The parasite Toxoplasma gondii is a highly ubiquitous parasite that latently infects an estimated 33% percent of the global population. Toxoplasmosis is of obvious clinical significance due to the extremely high risks to the unborn who contract it congenitally. Less attention has been given to a more subtle aspect of latent infection, which is the behavioral alterations that are commonly experienced by those who contract the parasite. Infected individuals commonly display a variety of altered behaviors, such as increased aggression and risk-seeking behaviors, depression, higher tendency towards suicide and self-directed violence, a loss of fear response, and a greatly increased …


Heterotopias Of Confinement: Zoo Architecture And The Loss Of Elephant Agency, Clayton Gill Beasley May 2025

Heterotopias Of Confinement: Zoo Architecture And The Loss Of Elephant Agency, Clayton Gill Beasley

Electronic Theses and Dissertations

Zoos functions as a site where human-animal relationships are structured through built environments that mediate control and representation. This study examines zoos as heterotopias—spaces where power and surveillance are negotiated between human observers and captive nonhuman animals. While zoos are often framed as spaces of conservation and education, their architectural design reinforces hierarchies of observation and confinement, shaping both human perceptions of nature and the lived experiences of nonhuman animals. This research focuses on Asian elephants as a critical species within this framework, exploring how their captivity illuminates tensions between human-imposed structures and nonhuman autonomy. Drawing from theories of heterotopia, …


Epidemiological Inferences Of The Impacts Of Winter Ticks On Moose Health, Krishna S. Woerheide May 2025

Epidemiological Inferences Of The Impacts Of Winter Ticks On Moose Health, Krishna S. Woerheide

Department of Entomology: Dissertations, Theses, and Student Research

The North American Moose (mooz in Anishinaabemowin, Ojibwe language, Alces alces), a culturally significant subsistence species for the Anishinaabe people, faces population decline due to calf predation, pathogen transmission, and parasitism by the winter tick (Dermacentor albipictus), all exacerbated by climate change. This study explores the impact of winter tick infestations on moose health, with a focus on natural mineral seeps as potential transmission hotspots in moose habitats within the Grand Portage Indian Reservation (Gichi Onigaming), on Isle Royale National Park (Minong), and within the 1854 Ceded Territory (greater northeastern Minnesota) - ancestral and present homelands of …


Evaluation Of Fecal Egg Counts (Fec) As A Viable Means To Estimate Ascaridia Galli, Heterakis Gallinarum And Capillaria Obsignata Burdens In Laying Hens, Alexander H. Moss May 2025

Evaluation Of Fecal Egg Counts (Fec) As A Viable Means To Estimate Ascaridia Galli, Heterakis Gallinarum And Capillaria Obsignata Burdens In Laying Hens, Alexander H. Moss

Animal Science Undergraduate Honors Theses

The control of gastrointestinal helminths, specifically Ascaridia galli, Heterakis gallinarum, and Capillaria obsignata, is a critical component of poultry health management, particularly in free-range and organic farming systems. Methods of estimating parasite burdens, such as post-mortem examinations, are not feasible for large-scale commercial operations due to their invasiveness and time requirements. This study aimed to evaluate the use of fecal egg counts (FEC) as a non-invasive and efficient method for estimating the worm burdens of these parasites in laying hens. A flock of 12 brown egg laying, spent, hens were individually penned on concrete. Fecal samples (both cecal and intestinal) …


Environmental Effects On Preweaning Calf Health, Lindsay L. Waechter-Mead May 2025

Environmental Effects On Preweaning Calf Health, Lindsay L. Waechter-Mead

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Neonatal calf health is an important factor in the productivity and profitability in the beef cattle industry, influencing preweaning survival, growth, and long-term performance. The overall objective of this thesis is to characterize risk factors that contribute to health issues in young beef calves. The first objective is to review literature exploring the environmental factors affecting preweaning morbidity and mortality, including hypothermia, precipitation, and wind. Management strategies such as the effect of environmental conditions of calving location, body condition score in dams at parturition, and the impacts of dystocia on calf health will also be discussed. Gaps in current research …


Stereotypic Behaviors And Physiological Indicators Of Stress Occur In Horses With Restricted Durations Of Turnout, Elizabeth E. Rodgers May 2025

Stereotypic Behaviors And Physiological Indicators Of Stress Occur In Horses With Restricted Durations Of Turnout, Elizabeth E. Rodgers

Department of Animal Science: Dissertations, Theses, and Student Research

The confinement of horses to stalls with limited or no turnout time restricts their natural roaming behaviors, which potentially increases stress and behavioral stereotypies. This study investigated the impact of turnout duration on behavioral and physiological stress indicators in healthy horses. Adult horses (n = 11) were enrolled in a 3 treatment (0-, 15-, and 60-minute turnout/day) Latin square design, including 3 12 d experimental periods over 6 weeks. Horses were acclimated to turnout (24.38 x 64.01m indoor arena) and stalls (2.74 x 4.87m - 3.66 x 3.66m) stalls prior to data collection on day 1. Experienced handlers managed …


Digestibility, Palatability, And Nutrient Composition Of Three Species Of Whole Prey In Three Species Of Snakes, Morgan R. Kienzle May 2025

Digestibility, Palatability, And Nutrient Composition Of Three Species Of Whole Prey In Three Species Of Snakes, Morgan R. Kienzle

Department of Animal Science: Dissertations, Theses, and Student Research

Snakes in human care often lack diversity in the diet. Rodents account for the majority of meals, while wild snakes are consuming a varied diet composed of multiple different whole prey species. By feeding only one species of prey, the chance of nutrient deficiencies and other health concerns increases. To promote variety in the managed diet, two commercially available whole prey species (mice (Mus musculus) and chicks (Gallus spp.)) and one novel species (Seba’s short-tailed bats (Carollia perspicillata)), were analyzed for their dry matter (DM), organic matter (OM), crude protein (CP), crude fat …


Methane Mitigation And Its Effects On Energy Utilization In Lactating Dairy Cattle, Shan C. Sherwood May 2025

Methane Mitigation And Its Effects On Energy Utilization In Lactating Dairy Cattle, Shan C. Sherwood

Department of Animal Science: Dissertations, Theses, and Student Research

Improved methane (CH4) mitigation strategies are needed to achieve the greenhouse gas emission goals of the dairy industry. The first experiment was conducted to evaluate the effects of feeding black cumin (Nigella sativa) seed oil and acetate (BCA) on CH4 production and whole-animal energy utilization. Twelve dry Jersey cows were used in a 2-period crossover design study with 28d periods. The BCA treatment was compared to a control diet with corn oil. Both BCA and corn oil were added to the base diet as a top dress. Results from this study did not show any …


Gonadotropin-Releasing Hormone Ii And Its Receptor: Novel Regulators Of Semen Quality And Follicular Function In Swine, Caitlin Elizabeth Ross May 2025

Gonadotropin-Releasing Hormone Ii And Its Receptor: Novel Regulators Of Semen Quality And Follicular Function In Swine, Caitlin Elizabeth Ross

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Pigs are the only mammalian livestock species encoding functional proteins for the second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II). In the first study, GnRHR-II localized to developing germ cells and the connecting piece of ejaculated spermatozoa. Concurrently, GnRH-II was most abundantly produced in the testis compared with other reproductive tract organs and was present within seminal plasma. Moreover, treatment of extended semen with GnRHR-II analogues altered sperm motility, morphology, and kinematics. For remaining studies, we utilized a swine line with ubiquitous knockdown of GnRHR-II (GnRHR-II KD) developed in our laboratory. The second study demonstrated that circulating GnRH-II …


Stomoxys Flies (Diptera, Muscidae) Are Competent Vectors Of Trypanosoma Evansi, Trypanosoma Vivax, And Other Livestock Hemopathogens, Julia W. Muita, Joel L. Bargul, John Mark O. Makwatta, Ernest M. Ngatia, Simon K. Tawich, Daniel K. Masiga, Merid N. Getahun May 2025

Stomoxys Flies (Diptera, Muscidae) Are Competent Vectors Of Trypanosoma Evansi, Trypanosoma Vivax, And Other Livestock Hemopathogens, Julia W. Muita, Joel L. Bargul, John Mark O. Makwatta, Ernest M. Ngatia, Simon K. Tawich, Daniel K. Masiga, Merid N. Getahun

All Peer-Reviewed Publications

Stomoxys flies are widely distributed and economically significant vectors of various livestock pathogens of veterinary importance. However, the role of Stomoxys spp. in pathogen transmission is poorly understood. Therefore, we studied the feeding patterns of these blood feeders collected from specific locations in Kenya to identify various vertebrate hosts they fed on, the livestock hemopathogens they carried, and to elucidate their role in pathogens transmission. Our findings show that field-collected Stomoxys flies carried several pathogens, including Trypanosoma spp., Anaplasma spp., and Theileria spp., which were also detected in the blood of sampled livestock, namely camels and cattle. The findings on …


Data From: The Dynamic Responses Of The Mouse Gut Microbiome To Dietary Supplementation With Various Anthocyanin-Rich Foods In The Context Of A Western Diet, Abby D. Benninghoff Apr 2025

Data From: The Dynamic Responses Of The Mouse Gut Microbiome To Dietary Supplementation With Various Anthocyanin-Rich Foods In The Context Of A Western Diet, Abby D. Benninghoff

Browse all Datasets

Background/Objectives: Anthocyanin (ACN)-rich foods may support gut health by modulating the microbiome and reducing inflammation, potentially lowering the risk of colitis-associated colorectal cancer. However, the dynamic response of the gut microbiome to ACN-rich diets is not well characterized, including in the context of structural and compositional differences among ACN-containing foods. This study examined the temporal response of the mouse gut microbiome to dietary ACNs, focusing on the impact of structural variation. We hypothesized that microbiome shifts would occur rapidly but require sustained dietary ACN intake to persist. Methods: C57BL/6J mice were fed the total Western diet (TWD) supplemented with freeze-dried …


Goat Tying Styles Impact On Performance Consistency With Rodeo Competitors In The National Intercollegiate Rodeo Association Ozark Region, Marie Baxley, C.A. Shea Porr, Thomas Powell Apr 2025

Goat Tying Styles Impact On Performance Consistency With Rodeo Competitors In The National Intercollegiate Rodeo Association Ozark Region, Marie Baxley, C.A. Shea Porr, Thomas Powell

Honors College Theses

Goat tying is a rodeo event involving dismounting a horse, and flanking and tying three legs of a goat. Flanking is defined as picking a goat up and setting it on the ground on its side. Flank/gather (FG) is a style where riders scoop the legs with the right hand and then place them in the left. Catch the flank (CF), stuffing (ST), and funneling (FN) are other methods that involve the goats’ legs landing together so the right hand is not needed for gathering. The objectives of this study were to determine 1) if flanking method affects performance, and …


The Effects Of Diluted Fungicide On Western Tiger Salamander (Ambystoma Mavortium) Health, Sage Veomett Apr 2025

The Effects Of Diluted Fungicide On Western Tiger Salamander (Ambystoma Mavortium) Health, Sage Veomett

Honors Program: Senior Projects (Public)

The Western Tiger Salamander (Ambystoma mavortium) is a species of salamander that is native to Nebraska. The Western Tiger Salamander population has been in decline, specifically in Southeast Nebraska (Ferraro, 2016). The purpose of this study was to determine whether low levels of Azoxystrobin fungicide deposited in the soil are harmful to the Western Tiger Salamander. In this study, soil in tanks containing pairs of Western Tiger Salamanders was treated with levels of Azoxystrobin 0.5x under the recommended label amount for usage. Fifteen salamanders in total were used. Four tanks of salamander pairs were treated with the Azoxystrobin …


Growing South Dakota (Spring 2025), College Of Agriculture, Food And Environmental Sciences Apr 2025

Growing South Dakota (Spring 2025), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Keeping South Dakota's food supply safe
[Page] 6 Controlling environmental listeria
[Page] 8 Inaugural 2+2 vet program class graduates
[Page] 10 Faculty honored for excellence
[Page] 12 New leader for agronomy, horticulture, plant science
[Page] 14 Students spend spring break in South Africa
[Page] 16 Hadacek offers advice to fellow graduates
[Page] 17 Park Interpretation and Management Club starts
[Page] 18 On the front lines with invasive species
[Page] 22 Director Karla Trautman retires
[Page] 24 Helping navigate precision ag
[Page] 26 Growing bigger, better beef
[Page] 28 Jackrabbits Then and Now


Emergence Of Transferable Daptomycin Resistance In Gram-Positive Bacteria, Tessa Marciniak, Lukas Kirchner, Silver A. Wolf, Birgit Walther, Thorsten Bischler, Justin Nyasinga, R Gunturu, Torsten Semmler, Tom Gräfenhan, Andrew Whitelaw Apr 2025

Emergence Of Transferable Daptomycin Resistance In Gram-Positive Bacteria, Tessa Marciniak, Lukas Kirchner, Silver A. Wolf, Birgit Walther, Thorsten Bischler, Justin Nyasinga, R Gunturu, Torsten Semmler, Tom Gräfenhan, Andrew Whitelaw

Pathology, East Africa

Daptomycin (DAP) is a last-resort antibiotic to treat infections by multiresistant Gram-positive pathogens, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci. DAP resistance and clinical treatment failure has been associated with adaptive chromosomal mutations, but so far not with transmissible resistance traits. Here we report for the first time an acquired DAP-R determinant (named drc) that we detected in a livestock-associated Mammaliicoccus sciuri isolate. drc consists of a two-gene operon (drcAB) that is controlled by an adjacent two-component system (drcRS). The DrcAB proteins, which mediate DAP inactivation, are similar to BceAB-like antimicrobial peptide transporters of Gram-positives, but are distinct from …


Study On Predisposing Factors And Control Measures Against Cryptosporidiosis In Buffalo Calves At Cattle Colony Hyderabad, Sindh, Pakistan, Ali Ul Murtaza, Abdul Latif Bhutto, Jam Kashif Zaman, Mansoor Tariq, Amjad Hussain Mirani, Riaz Ahmed Laghari, Pervez Ahmed Khoso, Muhammad Mohsen Rahimoon, Abdul Saboor, Fayaz Hussain Mar 2025

Study On Predisposing Factors And Control Measures Against Cryptosporidiosis In Buffalo Calves At Cattle Colony Hyderabad, Sindh, Pakistan, Ali Ul Murtaza, Abdul Latif Bhutto, Jam Kashif Zaman, Mansoor Tariq, Amjad Hussain Mirani, Riaz Ahmed Laghari, Pervez Ahmed Khoso, Muhammad Mohsen Rahimoon, Abdul Saboor, Fayaz Hussain

Journal of Bioresource Management

The current study looked at the variables affecting the frequency of Cryptosporidium Parvum in buffalo calves at the Hyderabad Cattle Colony. From calves, 100 fecal samples were taken in total, preserved, and analyzed using direct smear and Ziehl-Neelsen staining methods. 74 percent of the samples tested positive for Cryptosporidium Parvum. Disease rates were higher in Nili Ravi calves (86 %) compared to Kundhi calves (62 %), with a statistically significant difference (p


The Design, Construction, And Testing Of Mrna Vaccine Against Equine Herpes Virus, Bhawana Devkota Mar 2025

The Design, Construction, And Testing Of Mrna Vaccine Against Equine Herpes Virus, Bhawana Devkota

LSU Master's Theses

Equine Herpesvirus-1 (EHV 1) is a worldwide significant pathogen that causes respiratory illness, abortion, and neurological disorders in equine. Current vaccines, including live attenuated, inactivated, and subunit platforms, do not prevent viral latency, mucosal shedding, or cross-strain immunity, requiring alternative approaches. To address these gaps, this project explores the design, synthesis, and in vitro testing of an mRNA vaccine targeting immunogenic EHV 1 glycoprotein (gB, gC, gD, gG, and gM). Epitopes were computationally predicted using the Immune Epitope Database (IEDB), codon-optimized for Bos taurus, and cloned into pUCIDT vectors using SP6/T7 promoters. In vitro transcription (IVT) used nucleotide modifications …


Production And Characterization Of Muc13 Monoclonal Antibodies For Differential Expression In Cancers, Shabnam Malik, Daniel Zubieta, Mohammed Sikander Mar 2025

Production And Characterization Of Muc13 Monoclonal Antibodies For Differential Expression In Cancers, Shabnam Malik, Daniel Zubieta, Mohammed Sikander

Research Symposium

Background: MUC13 is a transmembrane glycoprotein that plays a critical role in the progression of various cancers, including liver, pancreatic, colon, and ovarian cancer. While its presence and implications in these malignancies have been recognized, the development of specific antibodies targeting MUC13 is limited. This study aims to produce a monoclonal antibody against MUC13 and evaluate its utility in assessing differential expressions associated with cancers progression.

Methodology: We employed hybridoma technology to generate specific monoclonal antibodies against MUC13. Mouse splenocytes were immunized with recombinant MUC13 protein and subsequently fused with myeloma cells to create hybridomas. Positive clones were selected …


Pharmacokinetics Of Liposomal Levamisole After Oral, Intramuscular, And Subcutaneousadministration In Sheep, Hasan Susar, İzzet Karahan Feb 2025

Pharmacokinetics Of Liposomal Levamisole After Oral, Intramuscular, And Subcutaneousadministration In Sheep, Hasan Susar, İzzet Karahan

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the pharmacokinetics of free and liposomal levamisole in sheep following single oral (O),intramuscular (IM), and subcutaneous (SC) administrations at a dose of 7.5 mg/kg. The study developed a levamisole liposome andinvestigated relevant parameters: particle size, zeta potential, polydispersity index, encapsulation efficiency, pH, and morphology. TheBangham method, a thin-film hydration technique, was employed to prepare the liposomal levamisole. Blood samples were collectedby jugular venipuncture in heparinized tubes immediately before each free and liposomal levamisole treatment and at 2.5, 5, 10, 15,30, 60, 90, 120, 240, 360, and 480 min and 12, 16, and 24 h after …


Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto Jan 2025

Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto

Karbala International Journal of Modern Science

The viral disease affects the whiteleg shrimp (Litopenaeus vannamei) and causes losses. Immunostimulants are recognized as a more environmentally friendly approach to disease management and antimicrobial properties of aquatic organisms. Watercress (Nasturtium officinale) is an aquatic plant species that contains antioxidant properties that can protect the organism from disease and boost the immune system. Shrimp rely heavily on innate immunity to combat infectious agents due to their absence of adaptive immunity. Peroxinectin and scavenger receptor class B (SRB) play essential roles in the shrimp defense system. This research employs a bioinformatic to assess the immunostimulatory potential …


Effect Of Feeding Fenugreek Extract And Encapsulated Lecithin On Finishing Cattle Performance, Carcass Characteristics, And Liver Abscesses, Sofia Suarez Lorences, Rebecca L. Mcdermott, Joshua R. Benton, Robert Bondurant, Willard Lemaster, James C. Macdonald, Galen E. Erickson Jan 2025

Effect Of Feeding Fenugreek Extract And Encapsulated Lecithin On Finishing Cattle Performance, Carcass Characteristics, And Liver Abscesses, Sofia Suarez Lorences, Rebecca L. Mcdermott, Joshua R. Benton, Robert Bondurant, Willard Lemaster, James C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

A feedlot study was conducted to evaluate the effects of adding fenugreek extract and encapsulated lecithin (Furst-McNess Company, Rockford, Illinois, United States) on finishing cattle performance, carcass characteristics, and liver abscesses. Treatments were applied as a 2×2 factorial that included a control diet with no additive; a diet containing fenugreek extract; a diet containing encapsulated lecithin; and a diet containing both fenugreek extract and encapsulated lecithin. Tylosin was not included in any diets to test natural occurrence of liver abscesses. No interactions were observed between fenugreek extract and encapsulated lecithin treatments but neither feed additive impacted intakes, …


Effects Of Allocation Frequency On Cattle Performance And Forage Utilization When Swath Grazing A Sorghum-Sudangrass Hybrid In Eastern Nebraska, Thomas E. Aquino, Mary E. Drewnoski, Pedro Henrique J. Fernandes Jan 2025

Effects Of Allocation Frequency On Cattle Performance And Forage Utilization When Swath Grazing A Sorghum-Sudangrass Hybrid In Eastern Nebraska, Thomas E. Aquino, Mary E. Drewnoski, Pedro Henrique J. Fernandes

Nebraska Beef Cattle Reports

Summary with Implications

A sorghum-sudangrass hybrid was swath grazed by growing steers (529 ± 18 lb) from November 2023 to January 2024. Treatments were offering new forage once (1X) or twice (2X) per week to determine the effect of allocation frequency on animal performance and forage utilization. Average daily gain was not impacted by treatment (1X = 0.45 ± 0.1 lb/day, 2X = 0.45 ± 0.1 lb/day). Pre-and post-graze biomass samples were collected to estimate forage utilization, which was not different between treatments (1X = 57.1 ± 3.6%, 2X = 61.1 ± 3.6%). However, there was a difference in carrying …


Growing South Dakota (Winter 2025), College Of Agriculture, Food And Environmental Sciences Jan 2025

Growing South Dakota (Winter 2025), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Native Plant Initiative on a mission
[Page] 4 Gees give back
[Page] 7 Christensen named assistant dean
[Page] 8 Fennell honored in plant biology
[Page] 9 New ag communication faculty
[Page] 10 SDSU Extension awards
[Page] 12 Veterinary medicine class welcomed
[Page] 13 Coulter honored
[Page] 14 National beef award winners
[Page] 15 Department celebrates 100 years
[Page] 16 Southeast Research Farm growing
[Page] 18 Saving the flock
[Page] 22 Energize conference inspires
[Page] 24 BeefSD helping producers
[Page] 25 SDSU Extension retirements
[Page] 26 New food safety testing service
[Page] 27 Bringing back bison
[Page] 28 Jackrabbits …