Viticulture and Oenology Commons^™

Revolutionizing Grapevine Breeding Programs: A Mobile Genome Editing System For Perennial Crops, Bridget Bolt

Theses and Dissertations--Plant and Soil Sciences

Grapevine (Vitis vinifera) faces unprecedented challenges from climate change, with up to 90% of current growing regions potentially becoming unsuitable for production within decades. Traditional breeding approaches require 25-30 years for variety development, creating a time constraint incompatible with the pace of environmental change. This research establishes the approach for a novel mobile RNA-based gene editing platform that addresses longstanding bottlenecks in grapevine improvement while preserving cultivar identity and reducing breeding timelines.

This research developed a three-component system for transgene-free gene editing in grapevine. First, a mobile CRISPR/Cas9 system was established using Agrobacterium rhizogenes transformation and the cut-and-dip …

From Code To Crops: Harnessing Bioinformatics And Artificial Intelligence (Ai) In Agricultural Omics, Lakshay Anand

Theses and Dissertations--Plant and Soil Sciences

Global agricultural faces numerous challenges, such as climate change, resource limitations, novel pests and diseases, increasing costs, and the ever-increasing human population. To tackle these challenges, we need innovative strategies that combine new technologies and data analytics approaches to enhance agricultural output, promote sustainable methods, and optimize resource allocation. The key to this innovation lies in understanding the complex molecular web within plants that governs their growth, defense, and adaptability mechanisms. By mastering this molecular network, we can cultivate crops that are more resilient, sustainable, and suitable for different climatic terrains. Moreover, studying the symbiotic relationship between plants and microorganisms …

In Pursuit Of Understanding Barrel Variation: Surveying The Lignocellulose Composition And Content Changes In Quercus Alba Barrel Staves During Cooperage And Bourbon Whiskey Maturation, Jarrad Wade Gollihue

Theses and Dissertations--Plant and Soil Sciences

Bourbon whiskey is a distilled spirit which is a uniquely American with several legal requirements including 1) the major grain in the mash is corn, 2) must be aged in a new charred white oak barrel, and 3) made in the United States of America. Kentucky is the whiskey historical home, producing most of the bourbon in the United States. Interest in whiskey has developed a need to understand more about the underlying chemistry of bourbon. The charred white oak barrel used in bourbon production is of substantial interest because the barrel has been implicated in giving American whiskey its …