Biosecurity Commons^™

The First Australian Co-Invasion Of Euwallacea Fornicatus, Fusarium Sp. [Af18] And Graphium Euwallaceae, Melinda Moir, Monica Kehoe, Dominie Wright, Stefania Bertazzoni, Peter Scott, Cuiping Wang, Elsie Kinnaird, Brenda A. Coutts

Biosecurity Research Articles

As international trade and travel increase around the world, invasive species detections and incursions are increasing in frequency. Here we document the first detection and incursion within Australia by the Polyphagous shot-hole borer (PSHB), Euwallacea fornicatus (Eichhoff, 1868) (Coleoptera: Curculionidae: Scolytinae), an ambrosia beetle, and two of the associated fungal species; Fusarium sp. [AF18], and Graphium euwallaceae. All three exotic species were detected in a confined region within Perth, Western Australia, and represent a co-invasion. This multispecies incursion into one of the world’s most remote cities highlights the rapid increase in biological invasions globally and the pressures that urban, agricultural, …

Managing Predators On Livestock Producing Properties In South Africa And Western Australia – Producer Perspectives, Tracey L. Kreplins, H A. Melville

Biosecurity Research Articles

Context

Predation impacts livestock farming enterprises worldwide. In South Africa and Western Australia, native and non-native predators negatively impact sheep and cattle farming enterprises. Aims

We hoped to compare the perspectives on predator impacts of livestock producers in relation to livestock predation in South Africa and Western Australia. Methods

Online and in-person surveys consisting of 26 questions were posed to farmers and pastoralists in both countries. The questions related to land ownership, property size, livestock management, predation impact (financial and livestock losses), control of predators, and other impacts on productivity. Key results

Livestock production properties are of a similar size …

Predator Management With A Single Bait? Use Of Eradicat For The Simultaneous Management Of Feral Cats, Red Foxes, And Wild Dogs, Tracey L. Kreplins, David Algar

Biosecurity Research Articles

Context

In Western Australia, there are three invasive predators that require management for agriculture and biodiversity protection, feral cats, wild dogs, and red foxes. These three predators often coexist in the same locality, suggesting potential efficacy gains can be made via simultaneous control. While Western Australian native species have evolved a high tolerance to poison baiting (1080), invasive predators have not. Therefore, landscape-scale baiting is commonly used for predator management. Aims

Eradicat baits designed for feral cat control have also been known to be consumed and control wild dogs and foxes. In this trial, we aimed to evaluate Eradicat as …

What’S The Best Way To Store Toxic 1080 Baits?, Heather M. Crawford, Ricarda Fenske, Gerard Smith, Teen Ryan, Jamie (Frog) Taylor, Daniel Carlisle, Debbie Dowden, Dean Butcher, Tracey L. Kreplins, James Miller, Malcolm S. Kennedy, Patricia A. Fleming

Biosecurity Research Articles

Being able to store toxic baits could allow livestock producers to target ‘hot spots’ of predator activity, supplementing or even replacing broadscale baiting, therefore avoiding potential risk of bait-resistant populations. We compared 1080 dose recovered from dried meat baits (DMB; camel, horse and kangaroo) and sausage baits stored by different methods (shed, locked transport box, freezer, cryo-vacuumed) to identify whether they were still lethal, and address concern that freezing/thawing could result in loss of the water-soluble 1080 toxicant. We developed a bait collection method to halt microbial activity (preserving 1080 dose), al-lowing collection of baits manufactured under field conditions by …

An Economic Analysis Of Cell-Fencing In Semi-Arid Rangelands, Stuart Dawson, Tracey L. Kreplins, Malcolm S. Kennedy, Santhi Wicks, Patricia A. Fleming

Biosecurity Research Articles

In Australia, livestock predation by dingoes (Canis familiaris) has contributed to what some livestock producers consider a dire situation for rangeland pastoralism, driving demand for cooperative regional-scale exclusion (‘cell’) fencing (i.e. pest-proof fences that encompass one or more individual properties) and landscape-scale predator control. The present case study predicted the effect of four cell-fences in the state of Western Australia (WA) on the gross margin of sheep (for meat or meat and wool) and cattle pastoral enterprises. We modelled the potential effects of the following four key variables: (1) four levels of commodity prices, (2) five levels of …

Genetic Diversity Of Soybean Dwarf Virus In Two Regions Of Mainland Australia, Benjamin S. Congdon, Murray Sharman, Monica Kehoe

Biosecurity Research Articles

Soybean dwarf virus (SbDV; family Tombusviridae, genus Luteovirus, species Luteovirus glycinis) is an RNA plant virus that is transmitted solely by aphids in a persistent, circulative and non-propagative manner. SbDV causes significant losses in cultivated Fabaceae, especially in subterranean clover (Trifolium subterraneum) pastures of mainland Australia. SbDV isolates are classified into four phenotypically distinguishable strains: YP, YS, DP, and DS. Y and D strains differ primarily in their host range, and P and S strains in their primary vector species. Genetically, Y and D strains separate into two clades in every genomic region except for …

An Assessment Of Dingo Ancestry In Camp Dogs In Western Australia, Tracey L. Kreplins, Danielle Stephens, N Laing, C Oskam

Biosecurity Research Articles

Hybridisation between Australian dingoes and domestic dogs is a controversial area of interest and research. An ongoing canine sterilisation programme in rural and remote Western Australia provided an opportunity to assess the dingo ancestry of camp dogs and opportunities for hybridisation. Blood samples were collected from 345 individual community dogs at 21 locations. Dogs were screened using 23 microsatellite loci and ancestry percentage assigned using an iterative Bayesian assignment algorithm. A single individual was a dingo, 96% were domestic dogs and 3.5% were hybrids. Camp dog and dingo hybridisation in these areas is of little concern in terms of conserving …

Postharvest Blown-Arc Plasma Treatment Did Not Reduce Fusarium Graminearum Or Its Mycotoxins In Field-Infested Wheat Grain, Maninder Kaur, M Kowalczyk, Daniel Huberli, Kirsty L. Bayliss

Biosecurity Research Articles

The increasing prevalence of Fusarium graminearum, a major causative pathogen of Fusarium head blight in cereal crops, leads to contamination of grain with mycotoxins, which necessitates the development of effective management strategies. This study investigated whether postharvest Blown-Arc plasma treatment could reduce F. graminearum and associated mycotoxins in field-infested grain. Postharvest Blown-Arc plasma treatment for 60 or 180 s was trialled to reduce Fusarium graminearum and deoxynivalenol (DON) mycotoxins in field-infested wheat grain. Changes in fungal components of the grain microbiome following the treatment were assessed using metabarcoding analysis of the nuclear ribosomal internal transcribed spacer gene region, …

Hymenopteran Parasitoids Of Fall Armyworm (Spodoptera Frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)) In Australia, With The Description Of Five New Species In The Families Braconidae And Eulophidae, Erinn P. Fagan-Jeffries, Melina M. Miles, Tareva-Chine C. Atkin-Zaldivar, Andy G. Howe, Siva Subramaniam, Donna M. Chambers, Frezzel Praise J Tadle, Saleh Adnan, Verni Sivasubramaniam, Alice Woodward, Helen Spafford, Brian Thistleton

Biosecurity Research Articles

Fall armyworm, Spodoptera frugiperda (J.E. Smith) is an invasive pest of agricultural crops including sweet corn and maize. The moth was first recorded in Australia in January 2020 and is now considered established in most states and territories, and research is underway to develop management strategies. Extensive rearing of S. frugiperda larvae and eggs occurred from March 2020 to April 2023 to understand the parasitoid complex present in Australia and identify potential biological control agents. We report here on the hymenopteran parasitoids reared during this period, which were identified using a combination of morphology and COI DNA barcoding, and provide …

Burial And Subsequent Growth Of Rigid Ryegrass (Lolium Rigidum) And Ripgut Brome (Bromus Diandrus) Following Strategic Deep Tillage, Catherine Borger, George Mwenda, Sarah Collins, Stephen Davies, Arslan Peerzada Dr, Andrew Van Burgel

Biosecurity Research Articles

Soil amelioration via strategic deep tillage is occasionally utilised within the conservation tillage systems to alleviate soil constraints, but its impact on weed seed burial and subsequent growth within the agronomic system is poorly understood. This study assessed the effects of different strategic deep tillage practices, including soil loosening (deep ripping), soil mixing (rotary spading), or soil inversion (mouldboard plough), on weed seed burial and subsequent weed growth, compared to a no-till control. The tillage practices were applied in 2019 at Yerecoin and Darkan, Western Australia, and data on weed seed burial and growth was collected during the following three-year …

Australian Cool-Season Pulse Seed-Borne Virus Research: 1. Alfalfa And Cucumber Mosaic Viruses And Less Important Viruses, Roger A C Jones, Benjamin S. Congdon

Biosecurity Research Articles

Here, we review the research undertaken since the 1950s in Australia’s grain cropping regions on seed-borne virus diseases of cool-season pulses caused by alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV). We present brief background information about the continent’s pulse industry, virus epidemiology, management principles and future threats to virus disease management. We then take a historical approach towards all past investigations with these two seed-borne pulse viruses in the principal cool-season pulse crops grown: chickpea, faba bean, field pea, lentil, narrow-leafed lupin and white lupin. With each pathosystem, the main focus is on its biology, epidemiology and management, …

An International Wheat Diversity Panel Reveals Novel Sources Of Genetic Resistance To Tan Spot In Australia, Julian Taylor, Dorthe Jorgensen, Caroline S. Moffat, Ken J. Chalmers, Rebecca Fox, Grant J. Hollaway, Melissa J. Cook, Stephen M. Neate, Pao Theen See, Manisha Shankar

Biosecurity Research Articles

Tan spot is a foliar disease in wheat caused by the fungal pathogen Pyrenophora tritici-repentis (Ptr) and has been reported to generate up to 50% yield losses under favourable disease conditions. Although farming management practices are available to reduce disease, the most economically sustainable approach is establishing genetic resistance through plant breeding. To further understand the genetic basis for disease resistance, we conducted a phenotypic and genetic analysis study using an international diversity panel of 192 wheat lines from the Maize and Wheat Improvement Centre (CIMMYT), the International Centre for Agriculture in the Dry Areas (ICARDA) and Australian (AUS) wheat …

P450-Mediated Resistance In Myzus Persicae (Sulzer) (Hemiptera: Aphididae) Reduces The Efficacy Of Neonicotinoid Seed Treatments In Brassica Napus, Lisa S. Kirkland, Evatt Chirgwin, Samantha E. Ward, Benjamin S. Congdon, Anthony Van Rooyen, Paul Umina

Biosecurity Research Articles

The prophylactic use of seeds treated with neonicotinoid insecticides remains an important means of controlling aphid pests in canola (Brassica napus) crops in many countries. Yet, one of the most economically important aphid species worldwide, the peach potato aphid (Myzus persicae), has evolved mechanisms which confer resistance to neonicotinoids, including amplification of the cytochrome P450 gene, CYP6CY3. While CYP6CY3 amplification has been associated with low-level resistance to several neonicotinoids in laboratory acute toxicity bioassays, its impact on insecticide efficacy in the field remains unresolved. In this study, we investigated the impact of CYP6CY3 amplification on …

Australian Cleotychini Planthoppers: Review Of The Genus Cleotyche Emeljanov, 1997 With Three New Species (Hemiptera: Fulgoromorpha: Dictyopharidae), Jérôme Constant, Linda Semeraro, Melinda L. Moir

Biosecurity Research Articles

The genus Cleotyche in the monotypic Australian planthopper tribe Cleotychini (Fulgoroidea: Dictyopharidae) is reviewed. The subgenus Griseotyche subgen. nov. is described to accommodate one species Cleotyche blanda Emeljanov, 2011 while the second species, C. mariae Emeljanov, 1997, is retained in the subgenus Cleotyche (Cleotyche) Emeljanov, 1997. Three new species of Cleotyche (Cleotyche) from Queensland, C. (Cleotyche) christinae sp. nov. from Cania Gorge National Park, C. (Cleotyche) francescoi sp. nov. from Eurimbula National Park and C. (Cleotyche) montana sp. nov. from Blackdown Tableland National Park are described and compared to the …

Insecticide Resistance In Australian Spodoptera Frugiperda (J.E. Smith) And Development Of Testing Procedures For Resistance Surveillance, Lisa Bird, Melina M. Miles, Adam Quade, Helen Spafford

Biosecurity Research Articles

Spodoptera frugiperda (J.E. Smith) is a highly invasive noctuid pest first reported in northern Australia during early 2020. To document current status of resistance in S. frugiperda in Australia, insecticide toxicity was tested in field populations collected during the first year of establishment, between March 2020 and March 2021. Dose-response was measured by larval bioassay in 11 populations of S. frugiperda and a susceptible laboratory strain of Helicoverpa armigera. Emamectin benzoate was the most efficacious insecticide (LC50 0.023μg/ml) followed by chlorantraniliprole (LC50 0.055μg/ml), spinetoram (LC50 0.098μg/ml), spinosad (LC50 0.526μg/ml), and methoxyfenozide (1.413μg/ml). Indoxacarb was the least toxic …

Virus Diseases Of Cereal And Oilseed Crops In Australia: Current Position And Future Challenges, Roger Jones, Murray Sharman, Piotr Trebicki, Solomon Maina, Benjamin S. Congdon

Biosecurity Research Articles

This review summarizes research on virus diseases of cereals and oilseeds in Australia since the 1950s. All viruses known to infect the diverse range of cereal and oilseed crops grown in the continent’s temperate, Mediterranean, subtropical and tropical cropping regions are included. Viruses that occur commonly and have potential to cause the greatest seed yield and quality losses are described in detail, focusing on their biology, epidemiology and management. These are: barley yellow dwarf virus, cereal yellow dwarf virus and wheat streak mosaic virus in wheat, barley, oats, triticale and rye; Johnsongrass mosaic virus in sorghum, maize, sweet corn and …

Virulence Assessment Of Australian Pyrenophora Tritici-Repentis Isolates, Pao Theen See, Kefei Chen, Kalai A. Marathamuthu, Nikki Schultz, Manisha Shankar, Caroline S. Moffat

Biosecurity Research Articles

The virulence of 57 Australian isolates of Pyrenophora tritici-repentis (Ptr), a necrotrophic fungal pathogen responsible for the major wheat disease tan spot, was assessed through plant infection assays. Isolates collected from the northern, southern, and western wheat-cropping regions of Australia were evaluated against 16 Australian bread wheat cultivars under controlled growth conditions. Following infection, the wheat panel displayed varying disease symptoms ranging from tiny necrotic specks to spreading chlorotic and necrotic lesions. Analysis of variance indicated that the wheat cultivar exhibited a greater effect on the disease response, explaining 62.7% of the variation, in comparison to the isolate …

Native And Non-Native Sources Of Carbohydrate Correlate With Abundance Of An Invasive Ant, Lori Lach, Benjamin Hoffmann, Melinda L. Moir

Biosecurity Research Articles

Invasive species threaten many ecological communities and predicting which communities and sites are invasible remains a key goal of invasion ecology. Although invasive ants often reach high abundances in association with plant-based carbohydrate resources, the source and provenance of these resources are rarely investigated. We characterized carbohydrate resources across ten sites with a range of yellow crazy ant abundance in Arnhem Land, Australia and New Caledonia to determine whether yellow crazy ant (Anoplolepis gracilipes) abundance and trophic position correlate with carbohydrate availability, as well as the relative importance of native and non-native sources of carbohydrates to ant diet. …

Analysis Of Mutations In West Australian Populations Of Blumeria Graminis F. Sp. Hordei Cyp51 Conferring Resistance To Dmi Fungicides, Madeline A. Tucker, Francisco J. Lopez-Ruiz, Hans J. Cools, Jonathon G L Mullins, Kithsiri W. Jayasena, Richard P. Oliver

Biosecurity Research Articles

BACKGROUND

Powdery mildew caused by Blumeria graminis f. sp. hordei (Bgh) is a constant threat to barley production but is generally well controlled through combinations of host genetics and fungicides. An epidemic of barley powdery mildew was observed from 2007 to 2013 in the West Australian grain belt.

RESULTS

We collected isolates across Australia, examined their sensitivity to demethylation inhibitor (DMI) fungicides and sequenced the Cyp51B target gene. Five amino acid substitutions were found, of which four were novel. The most resistant haplotypes increased in prevalence from 0% in 2009 to 16% in 2010 and 90% in 2011. …

Application Of Loop-Mediated Isothermal Amplification In An Early Warning System For Epidemics Of An Externally Sourced Plant Virus, Benjamin S. Congdon, Paul Matson, Farhana Begum, Monica Kehoe, Brenda A. Coutts

Biosecurity Research Articles

Restricting Turnip yellows virus (TuYV) spread in canola (Brassica napus) crops often relies upon the application of systemic insecticides to protect young vulnerable plants from wide-scale green-peach aphid (GPA; Myzus persicae) colonization and subsequent virus infection. For these to be applied at the optimal time to ensure they prevent epidemics, growers would need to be forewarned of incoming viruliferous aphid migration and colonization. This study was conducted to field validate a loop-mediated isothermal amplification (LAMP) protocol designed to detect TuYV in aphids caught on traps and develop an early warning system for TuYV epidemics. Double-sided yellow sticky traps were deployed …

The Survival Of Mediterranean Fruit Fly (Diptera: Tephritidae) Over Winter In Western Australia, Touhidur Rahman, Sonya Broughton

Biosecurity Research Articles

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is one of the most economically important pest insects of fruit crops worldwide. Mediterranean fruit fly can cause up to 100% crop loss in susceptible fruit. In order to formulate best management practices, it is critical to understand how Mediterranean fruit fly overwinters in a given geographical location and bridge the gap between autumn and spring populations. In this study, we evaluated the overwintering potential of Mediterranean fruit fly immature and adult stages in two locations in Perth Hills, Western Australia. We also monitored wild adult Mediterranean fruit fly populations for …

Development And Testing Of A Standardized Method To Estimate Honeydew Production, Melinda L. Moir, Michael Renton, Benjamin Hoffmann, Mei Chen Leng, Lori Lach

Biosecurity Research Articles

Honeydew production by Hemiptera is an ecologically important process that facilitates mutualisms and increases nutrient cycling. Accurate estimates of the amount of honeydew available in a system are essential for quantifying food web dynamics, energy flow, and the potential growth of sooty mould that inhibits plant growth. Despite the importance of honeydew, there is no standardized method to estimate its production when intensive laboratory testing is not feasible. We developed two new models to predict honeydew production, one based on insect body mass and taxonomic family, and one based on body mass and life stage. We tested the accuracy of …

Loci On Chromosomes 1a And 2a Affect Resistance To Tan (Yellow) Spot In Wheat Populations Not Segregating For Tsn1, Manisha Shankar, Dorthe Jorgensen, Julian Taylor, Ken J. Chalmers, Rebecca Fox, Grant J. Hollaway, Stephen M. Neate, Mark S. Mclean, Elysia Vassos, Hossein Golzar, Rob Loughman, Diane E. Mather

Biosecurity Research Articles

Tan spot (syn. yellow spot and yellow leaf spot) caused by Pyrenophora tritici-repentis is an important foliar disease of wheat in Australia. Few resistance genes have been mapped in Australian germplasm and only one, known as tsn1 located on chromosome 5B, is known in Australian breeding programs. This gene confers insensitivity to the fungal effector ToxA. The main aim of this study was to map novel resistance loci in two populations: Calingiri/Wyalkatchem, which is fixed for the ToxA-insensitivity allele tsn1, and IGW2574/Annuello, which is fixed for the ToxA-sensitivity allele Tsn1. A second aim was to combine …

Development Of Genetic Ssr Markers In Blumeria Graminis F. Sp. Hordei And Application To Isolates From Australia, Madeline Tucker, C S. Moffat, Simon R. Ellwood, K C. Tan, Kithsiri Jayasena, Richard P. Oliver

Biosecurity Research Articles

The barley powdery mildew pathogen, Blumeria graminis f. sp. hordei (Bgh), exists in numerous haplotypes and displays significant differences in fungicide sensitivity. It causes considerable yield losses throughout the world. Microsatellite SSRs are useful tools to study the population level and biogeographic aspects of intraspecific diversity, but so far none have been defined for Bgh. Here, eight polymorphic microsatellite loci were identified and characterized. Primer pairs amplifying the loci were then applied to 111 isolates of Bgh from Australia. The number of alleles per locus ranged from 4 to 13, and Nei's genetic diversity ranged from 0·25 to 0·76. The …

Quantitative Trait Loci For Seedling And Adult Plant Resistance To Stagonospora Nodorum In Wheat, Manisha Shankar, Esther Walker, Hossein Golzar, Rob Loughman, R E. Wilson, Michael Francki

Biosecurity Research Articles

Stagonospora nodorum blotch (SNB) caused by Stagonospora nodorum is a severe disease of wheat (Triticum aestivum) in many areas of the world. S. nodorum affects both seedling and adult plants causing necrosis of leaf and glume tissue, inhibiting photosynthetic capabilities, and reducing grain yield. The aims of this study were to evaluate disease response of 280 doubled haploid (DH) individuals derived from a cross between resistant (6HRWSN125) and susceptible (WAWHT2074) genotypes, compare quantitative trait loci (QTL) for seedling and adult plant resistance in two consecutive years, and assess the contribution of QTL on grain weight. Flag leaves and …

Should Australia Export Its Native Birds?, Ross Kingwell

Biosecurity Research Articles

Commercial export from Australia of native birds, wild or captive bred, is prohibited. This paper firstly describes the current legislation and regulations that restrict the export of native birds and discusses why governments have adopted such a regulatory approach to bird species preservation. Secondly, the paper reviews the debate concerning the export ban, pointing out strengths and weaknesses in arguments and indicating the important role of CITES. Lastly, the paper outlines a new case for a conditional lifting of the ban, whereby DNA fingerprinting is used to establish transferable property rights to overcome a main source of market failure in …