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Full-Text Articles in Agriculture

Increased Bundle-Sheath Leakiness Of Co2 During Photosynthetic Induction Shows A Lack Of Coordination Between The C4 And C3 Cycles, Yu Wang, Samantha S. Stutz, Carl J. Bernacchi, Ryan A. Boyd, Donald R. Ort, Stephen P. Long Dec 2022

Increased Bundle-Sheath Leakiness Of Co2 During Photosynthetic Induction Shows A Lack Of Coordination Between The C4 And C3 Cycles, Yu Wang, Samantha S. Stutz, Carl J. Bernacchi, Ryan A. Boyd, Donald R. Ort, Stephen P. Long

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Use of a complete dynamic model of NADP-malic enzyme C4 photosynthesis indicated that, during transitions from dark or shade to high light, induction of the C4 pathway was more rapid than that of C3, resulting in a predicted transient increase in bundle-sheath CO2 leakiness (ϕ). Previously, ϕ has been measured at steady state; here we developed a new method, coupling a tunable diode laser absorption spectroscope with a gas-exchange system to track ϕ in sorghum and maize through the nonsteady-state condition of photosynthetic induction. In both species, ϕ showed a transient increase to > 0.35 before declining to a steady state …


A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk Dec 2022

A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk

Department of Agronomy and Horticulture: Faculty Publications

Background: Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage. Results: This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl2, …


Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson Aug 2022

Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Arbuscular mycorrhizal fungi (AMF) remain a vital obligate symbiont of nearly all plants. It is well established that the symbiosis between AMF and host plant improves plant nutrient acquisition, alleviates abiotic and biotic environmental stressors, defends against plant pathogens, and contributes to overall plant fitness and productivity through modification of the soil habitat. Modifications include increased soil aggregation and stability, carbon sequestration through provision of fungal wall precursors to soil organic matter (SOM) formation, and enhanced nutrient cycling in the mycorrhizosphere. The goal of this dissertation was to assess how AMF respond to nitrogen (N) fertilization regimes in maize cropping …


Substantial Carbon Loss Respired From A Corn-Soybean Agroecosystem Highlights The Importance Of Careful Management As We Adapt To Changing Climate, Caitlin E. Moore, Christy D. Gibson, Guofang Miao, Evan C. Dracup, Nuria Gomez-Casanovas, Michael D. Masters, Jesse Miller, Adam C Von Haden, Tilden Meyers, Evan H. Delucia, Carl J. Bernacchi Jan 2022

Substantial Carbon Loss Respired From A Corn-Soybean Agroecosystem Highlights The Importance Of Careful Management As We Adapt To Changing Climate, Caitlin E. Moore, Christy D. Gibson, Guofang Miao, Evan C. Dracup, Nuria Gomez-Casanovas, Michael D. Masters, Jesse Miller, Adam C Von Haden, Tilden Meyers, Evan H. Delucia, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Understanding agroecosystem carbon (C) cycle response to climate change and management is vital for maintaining their long-term C storage. We demonstrate this importance through an in-depth examination of a ten-year eddy covariance dataset from a corn-corn-soybean crop rotation grown in the Midwest United States. Ten-year average annual net ecosystem exchange (NEE) showed a net C sink of -0.39 Mg C ha-1 yr-1. However, NEE in 2014 and 2015 from the corn ecosystem was 3.58 and 2.56 Mg C ha-1 yr-1, respectively. Most C loss occurred during the growing season, when photosynthesis should dominate and C fluxes should reflect a net …