Agriculture | Open Access Articles | Digital Commons Network™

Boxwood Blight, Fulya Baysal-Gurel, Prabha Liyanapathiranage

Extension Publications

No abstract provided.

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Wild Stimulant Production Methods For American Ginseng Farms In Tennessee, Shannon Smith, John Dubois, Nate Phillips, Arvazena Clardy

Extension Publications

No abstract provided.

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Development Of A Laser Capture Microscope-Based Single-Cell-Type Proteomics Tool For Studying Proteomes Of Individual Cell Layers Of Plant Roots, Yingde Zhu, Hui Li, Sarabjit Bhatti, Suping Zhou, Yong Yang, Tara Fish, Theodore W. Thannhauser

Agricultural and Environmental Sciences Faculty Research

Single-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution. However, the methodology for this type of research has not been well-developed. This paper reports developing a workflow of laser capture microdissection (LCM) followed by gel-liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots. Thin-sections (~10-μm thick, 10 sections per root tip) were prepared for root tips of tomato germinating seedlings. Epidermal and cortical cells (5000–7000 cells per tissue type) were isolated under a LCM microscope. Proteins were isolated and then separated by SDS–polyacrylamide …

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Large-Plot Field Studies To Assess Impacts Of Newer Insecticides On Non-Target Arthropods In Western U.S. Orchards, Peter W. Shearer, Kaushalya G. Amarasekare, Steven P. Castagnoli, Elizabeth H. Beers, Vincent P. Jones, Nicholas J. Mills

Agricultural and Environmental Sciences Faculty Research

The non-target impacts of two reduced risk insecticides, chlorantraniliprole and spinetoram, were evaluated for two years in Oregon pear and California walnut orchards. Experiments were conducted in large replicated plots (approximately 0.25–0.4ha) to assess the impact of these two insecticides on natural enemies of secondary pests when applied against codling moth, Cydia pomonella. Cumulative insect days (CID) of secondary pests and natural enemies were calculated from leaf samples, plant volatile traps, beat trays or cardboard trunk bands. Ratios of natural enemies and prey were also calculated. Results from these field studies demonstrate that applications of chlorantraniliprole can reduce abundance of …

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Nontarget Effects Of Orchard Pesticides On Natural Enemies: Lessons From The Field And Laboratory, Elizabeth H. Beers, Nicholas J. Mills, Peter W. Shearer, David R. Horton, Eugene R. Milickzy, Kaushalya G. Amarasekare, Lessando M. Gontijo

Agricultural and Environmental Sciences Faculty Research

The nontarget effects of insecticide programs used to control codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), were studied in large-plot field trials in apples, pears, and walnuts in the western United States. We assessed the health of the natural enemy community by sampling the abundance of natural enemies and by monitoring for outbreaks of secondary pests. The insecticides used in the field tests overlapped those tested in laboratory bioassays. Using these parallel lab and field studies, we examined two hypotheses: 1) pesticides found to have negative effects on natural enemy fitness in laboratory bioassays will predict reductions in natural enemy …

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Cost Minimization Of Supplying Biomass For Ethanol Biorefineries, Yayuan Jin, Prabodh Illukpitiya

Agricultural and Environmental Sciences Faculty Research

A major hurdle facing commercial biofuel production is the cost of producing the feedstock. Since biofuel feedstock is bulky in nature, a large proportion of cost needed to be allocated for harvesting and transportation of feedstock. Economic viability of ethanol production from cellulosic feedstock depends in part of the cost to produce, harvest and deliver feedstock to the ethanol production facilities. A well-developed harvesting and transportation system does not exist for most feedstock. Hence to determine accurate estimation of the harvest, transportation and storage costs is important in ethanol production. The objectives of the study are to determine the optimal …

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Relative Growth Of Carcass Tissues Of Goat Kids From Five Breed Types Finished On Pasture Or Feedlot, Raquel Vasconcelos Lourençon, Heraldo Cesar Gonçalves, Paulo Roberto De Lima Meirelles, Richard Browning Jr., Maria Lenira Leite-Browning, Andréia Cristina Toniolo Chávari, Raquel Ornelas Marques, Gil Ignacio Lara Canizares, Natalia Santos Leal, Helen Fernanda Barros Gomes

Agricultural and Environmental Sciences Faculty Research

he aim of this study was to evaluate the effect of cross breeding, finishing system, and gender on the relative growth of carcass tissues of dairy kids. Seventy eight kids (39 male and 39 female) from five breed types were used: Alpine; ½ Boer + ½ Alpine (½ BA); ½ Nubian + ½ Alpine (½ ANA); ¾ Boer + ¼ Alpine (¾ BA); and ½ Nubian + ¼ Boer + ¼ Alpine (TC). Kids were distributed into two finishing systems: in pasture with doe (FS1) and weaned in feedlot (FS2). Kids were slaughtered at a mean age of 128.4 ± …

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