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Three single nucleotide polymorphisms (SNPs) at -660bp (deletion of a single guanine base), -380bp (T>C), -69bp (C>T) were identified by sequencing cDNA of CYB5 genes in different Chinese and Foreign pigs. After subsequent detection using microsatellite, pyrosequencing and restriction fragment length polymorphism (RFLP) techniques in large populations of different pig breeds, we found that the mutations in all the SNP sites were favorable in Chinese pigs except LiCha black pigs which were crossed with Landrace pigs before, whereas the wild type nucleotides were favorable in Foreign pigs including Large White, Landrace and Duroc pigs. Therefore, we conclude that these SNPs …

The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and codingregions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Comparative analysis of the promoter region among 20 species including pig revealed four conserved regions that harbor transcriptional factors involved in adipose differentiation. Using a pooled DNA sequencing approach, we discovered 39 single nucleotide polymorphisms (SNPs) in the pig FTO gene and four of them were …