66th National Poultry Breeder Roundtable Poster_Jibin Zhang.Pdf, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont
May 2017
66th National Poultry Breeder Roundtable Poster_Jibin Zhang.Pdf, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont
Jibin Zhang
Fast-growing modern broilers are susceptible to cardiac arrhythmia and heart failure. To understand the developmental regulation related to these problems, cardiac gene expression in Illinois and Ross broilers was investigated at 6 and 21 days post hatch (dph) using RNA-seq (n=5-6 for each group). Because Illinois is a legacy broiler line with isometric heart growth, and Ross is a modern broiler line with a negative allometric heart growth, comparison of gene expression between the two lines and the two time points provides an avenue to understand genetic regulation underlying their different cardiac development.
Sequence data were mapped to the Galgal5 …
Pag 2017 Poster _ Jibin Zhang.Pdf, Jibin Zhang, Michael G. Kaiser, Melissa S. Herrmann, Rodrigo A. Gallardo, David A. Bunn, Terra R. Kelly, Jack C. M. Dekkers, Huaijun Zhou, Susan J. Lamont
Jan 2017
Pag 2017 Poster _ Jibin Zhang.Pdf, Jibin Zhang, Michael G. Kaiser, Melissa S. Herrmann, Rodrigo A. Gallardo, David A. Bunn, Terra R. Kelly, Jack C. M. Dekkers, Huaijun Zhou, Susan J. Lamont
Jibin Zhang
Velogenic Newcastle disease virus (NDV) causes global economic losses in the poultry industry. Although proper vaccination does prevent outbreaks of ND, genetic selection for enhanced resistance to NDV offers a complementary approach to disease prevention. Understanding the gene expression changes induced by ND infection will help to elucidate mechanisms of host resistance and susceptibility.
Two distinct and highly inbred chicken lines - Fayoumi and Leghorn - were studied as relatively ND-resistant and susceptible models, respectively. Equal numbers of birds from each line were inoculated with LaSota strain NDV (or saline control) through nasal and ocular routes at 21 days of …
Gene Expression Response To Heat Stress In Two Broiler Lines, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont
Mar 2016
Gene Expression Response To Heat Stress In Two Broiler Lines, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont
Jibin Zhang
The fast growth rate and increased body weight of modern broilers makes them susceptible to heart failure and sensitive to heat stress. To find the genetic causes of these problems, gene expression was measured in hearts of two broiler lines that differed in their response to heat stress. Each line was separated into two groups that were kept under heat stress or at ambient temperature. The comparison of gene expression between the two lines and heat treatments suggests that expression of 325 genes were significantly changed in modern broilers under heat stress, while only 3 genes changed their expression in …
Differential Expression Of Cyb5a In Chinese And European Pig Breeds Due To Genetic Variations In The Promoter Region..Pdf, Ying Bai, Jibin Zhang, Y Xue, Yl Peng, Gang Chen, Meiying Fang
Dec 2015
Differential Expression Of Cyb5a In Chinese And European Pig Breeds Due To Genetic Variations In The Promoter Region..Pdf, Ying Bai, Jibin Zhang, Y Xue, Yl Peng, Gang Chen, Meiying Fang
Jibin Zhang
Cytochrome b5 (CYB5A) is an important electron transfer protein with homologues in a number of different organisms. In pigs, CYB5A is related to boar taint because of its role in androstenone biosynthesis. To determine the variety of CYB5A expression in pig breeds, genetic variations in the porcine CYB5A promoter region in both Chinese and European pig breeds were examined. Three single nucleotide polymorphisms (NC_010443.4:
g.165901487delG, g.165901767T>C and g.165902078C>T) were identified in the porcine CYB5A promoter region. These SNPs occurred in different frequencies in Chinese and European pigs. Chinese pigs were primarily haplotype B (denoted as delG-C-T: the position of nt …
Differential Expression Of Cell Cycle Regulators During Hyperplastic And Hypertrophic Growth Of Broiler Subcutaneous Adipose Tissue.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Paula Chen, Michael Davis, Kichoon Lee
May 2015
Differential Expression Of Cell Cycle Regulators During Hyperplastic And Hypertrophic Growth Of Broiler Subcutaneous Adipose Tissue.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Paula Chen, Michael Davis, Kichoon Lee
Jibin Zhang
Hyperplasticgrowth and hypertrophicgrowth within adiposetissue is tightly associated with cellcycle activity. In this study, CCNG2 and CDKN2C were found to be correlated with cellcycle inhibition during fat cell differentiation, whereas CCND3, CCNA1, and ANAPC5 were positively associated with cellcycle activity during fat cell proliferation after selection based on GEO datasets available on the NCBI website. The findings were validated through comparison of expressions of these genes among different tissues/fractions in broiler chickens and time points during primary cell culture using quantitative real-time PCR. Development of broilersubcutaneousadiposetissue was investigated on embryonic days 15 and 17 and on post-hatch days 0, 5, …
Identification Of Ctla2a, Defb29, Wfdc15b, Serpina1f And Mup19 As Novel Tissue-Specific Secretory Factors In Mouse..Pdf, Jibin Zhang, Jinsoo Ahn, Yeunsu Suh, Seongsoo Hwang, Michael E. Davis, Kichoon Lee
May 2015
Identification Of Ctla2a, Defb29, Wfdc15b, Serpina1f And Mup19 As Novel Tissue-Specific Secretory Factors In Mouse..Pdf, Jibin Zhang, Jinsoo Ahn, Yeunsu Suh, Seongsoo Hwang, Michael E. Davis, Kichoon Lee
Jibin Zhang
Secretoryfactors in animals play an important role in communication between different cells, tissues and organs. Especially, the secretoryfactors with specific expression in one tissue may reflect important functions and unique status of that tissue in an organism. In this study, we identified potential tissue-specificsecretoryfactors in the fat, muscle, heart, lung, kidney and liver in the mouse by analyzing microarray data from NCBI's Gene Expression Omnibus (GEO) public repository and searching and predicting their subcellular location in GeneCards and WoLF PSORT, and then confirmed tissue-specific expression of the genes using semi-quantitative PCR reactions. With this approach, we confirmed 11 lung, 7 …
Differential Expression Of Cyclin G2, Cyclin Dependent Kinase Inhibitor 2c And Peripheral Myelin Protein 22 Genes During Adipogenesis..Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Jinsoo Ahn, Michael E. Davis, Kichoon Lee
May 2014
Differential Expression Of Cyclin G2, Cyclin Dependent Kinase Inhibitor 2c And Peripheral Myelin Protein 22 Genes During Adipogenesis..Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Jinsoo Ahn, Michael E. Davis, Kichoon Lee
Jibin Zhang
Increase of fat cells (FCs) in adipose tissue is attributed to proliferation of preadipocytes or immature adipocytes in the early stage, as well as adipogenic differentiation in the later stage of adipose development. Although both events are involved in the FC increase, they are contrary to each other, because the former requires cell cycle activity, whereas the latter requires cell cycle withdrawal. Therefore, appropriate regulation of cell cycle inhibition is critical to adipogenesis. In order to explore the important cell cycle inhibitors and study their expression in adipogenesis, we adopted a strategy combining the Gene Expression Omnibus (GEO) database available …
Jibin Zhang's Ppt For Midwest Meeting.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Michael E. Davis, Kichoon Lee
Mar 2014
Jibin Zhang's Ppt For Midwest Meeting.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Michael E. Davis, Kichoon Lee
Jibin Zhang
Increasing numbers of fat cells in adipose tissue are attributed to proliferation of preadipocytes or immature adipocytes in the early stages as well as adipogenic differentiation in the later stages of adipogenesis. Although both events are involved in the increase in fat cell numbers, they are contrary to each other in that the former requires cell cycle activity whereas the latter requires cell cycle withdrawal. Therefore, appropriate regulation of cell cycle inhibition is critical to adipogenesis.
To explore the important cell cycle inhibitors and study their expression in adipogenesis, a strategy was adopted that combined the Gene Expression Omnibus (GEO) …
A Comprehensive Microrna Expression Profile Of The Backfat Tissue From Castrated And Intact Full-Sib Pair Male Pigs.Pdf, Ying Bai, Jinming Huang, Gang Liu, Jibin Zhang, Jianying Wang, Chengkun Liu, Meiying Fang
Jan 2014
A Comprehensive Microrna Expression Profile Of The Backfat Tissue From Castrated And Intact Full-Sib Pair Male Pigs.Pdf, Ying Bai, Jinming Huang, Gang Liu, Jibin Zhang, Jianying Wang, Chengkun Liu, Meiying Fang
Jibin Zhang
Background: It is widely known that castration has a significant effect on the accumulation of adipose tissue. microRNAs (miRNAs) are known to be involved in fat deposition and to be regulated by the androgen-induced androgen receptor (AR). However, there is little understanding of the relationship between miRNAs and fat deposition after castration. In this study, the high-throughput SOLiD sequencing approach was used to identify and characterize miRNA expression in backfat from intact and castrated full-sib male 23-week-old pigs. The patterns of adipogenesis and fat deposition were compared between castrated and intact male pigs.
Results: A total of 366 unique miRNA …
Genetic Character Of Cyb5 Gene Among Different Pig Breeds.Pdf, Ying Bai, Jibin Zhang, Meiying Fang
Oct 2013
Genetic Character Of Cyb5 Gene Among Different Pig Breeds.Pdf, Ying Bai, Jibin Zhang, Meiying Fang
Jibin Zhang
Three single nucleotide polymorphisms (SNPs) at -660bp (deletion of a single guanine base), -380bp (T>C), -69bp (C>T) were identified by sequencing cDNA of CYB5 genes in different Chinese and Foreign pigs. After subsequent detection using microsatellite, pyrosequencing and restriction fragment length polymorphism (RFLP) techniques in large populations of different pig breeds, we found that the mutations in all the SNP sites were favorable in Chinese pigs except LiCha black pigs which were crossed with Landrace pigs before, whereas the wild type nucleotides were favorable in Foreign pigs including Large White, Landrace and Duroc pigs. Therefore, we conclude that these SNPs …
Expression Analysis Of Hif-1a And Hif-2a Genes In Tibetan Chicken Under Normoxia And Hypoxia.Pdf, Yunzhou Yang, Ying Bai, Xinxing Dong, Jibin Zhang, Meiying Fang
Nov 2011
Expression Analysis Of Hif-1a And Hif-2a Genes In Tibetan Chicken Under Normoxia And Hypoxia.Pdf, Yunzhou Yang, Ying Bai, Xinxing Dong, Jibin Zhang, Meiying Fang
Jibin Zhang
Tibetan chicken is one of those chicken breeds that could adapt to high altitude and low oxygen pressure environment; it owes to an integrative genetic mechanism for hypoxia adaptability compared to lowland chicken breeds. HIF-1α and HIF-2α are the central factors playing important roles in maintaining organisms’s oxygen homeostasis. In this study, the expression of HIF-1α and HIF-2α genes were investigated by Real Time-PCR in Tibetan chicken and Dwarf chicken embryo brain tissue under normoxia (21% oxygen concentration) and hypoxia (13% oxygen concentration). The results showed that Tibetan chicken always had lower mortality than Dwarf chicken during the incubation. We …
Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang
Nov 2010
Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang
Jibin Zhang
The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and codingregions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Comparative analysis of the promoter region among 20 species including pig revealed four conserved regions that harbor transcriptional factors involved in adipose differentiation. Using a pooled DNA sequencing approach, we discovered 39 single nucleotide polymorphisms (SNPs) in the pig FTO gene and four of them were …
3hsds1和17hsds7基因5'上游区转录活性研究.Pdf, Xinxing Dong, Yi Xin, Ying Bai, Yunzhou Yang, Jibin Zhang, Meiying Fang, Gang Chen
Dec 2009
3hsds1和17hsds7基因5'上游区转录活性研究.Pdf, Xinxing Dong, Yi Xin, Ying Bai, Yunzhou Yang, Jibin Zhang, Meiying Fang, Gang Chen
Jibin Zhang
3β-hydroxysteroid dehydrogenases (HSDs) and 17βHSDs are pivotal enzymes involved in synthesis, activation and deactivation of steroid hormones. Therefore, they play an important role to maintain homeostasis of hormone and regulate the synthesis and metabolism of hormones, and they are also related to metabolism of androstenone which can cause boar taint in male pigs. Through ligation of different PCR fragments of these genes from Large White pigs to pGL3-basic plasmid and subsequent transient transfection of constructed vectors into HepG2 cell culture, we found that the fragment from -1038bp to 45bp in porcine 3βHSD gene and the fragment from -966bp to 39bp …
