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Articles 1741 - 1770 of 1851

Full-Text Articles in Life Sciences

Performance Of Mouse Lines Divergently Selected For Heat Loss When Exposed To Different Environmental Temperatures. Ii. Feed Intake, Growth, Fatness, And Body Organs, P. M. Kgwatalala, Merlyn K. Nielsen Jan 2004

Performance Of Mouse Lines Divergently Selected For Heat Loss When Exposed To Different Environmental Temperatures. Ii. Feed Intake, Growth, Fatness, And Body Organs, P. M. Kgwatalala, Merlyn K. Nielsen

Department of Animal Science: Faculty Publications

Mouse populations differing in metabolic rate have been developed through selection for high (MH) and low (ML) heat loss, along with the unselected controls (MC). Objectives of the study were to compare the MH, ML, and MC lines for feed intake, growth, body fatness, and organ weights when reared at 12, 22, and 31°C, and investigate potential line × environment interactions. Feed intake was recorded weekly from 3 to 9 wk of age, and BW at 3, 6, and 9 wk of age. Body fat percent and organ weights were measured at 9 wk of age. No line × environment …

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Molecular Characterization Of Immunoglobins A, G, And M Heavy Chains In The Atlantic Bottlenose Dolphin, Tursiops Truncatus, Holly Ann Gefroh Jan 2004

Molecular Characterization Of Immunoglobins A, G, And M Heavy Chains In The Atlantic Bottlenose Dolphin, Tursiops Truncatus, Holly Ann Gefroh

MUSC Theses and Dissertations

The dolphin immune system is not well characterized, unlike the human and mouse immune systems. As a sentinel species of the marine ecosystem, an understanding of the dolphin immune system will not only contribute to conservation and management of the species, but also contribute to an understanding of the status of the marine ecosystem. cDNA sequences encoding IgA and IgG heavy chains and IgM transmembrane segment of the Atlantic bottlenose dolphin, Tursiops truncatus, were obtained using a combination of reserve transcription of mRNA, PCR amplification using degenerate primers, and 3' and VH-FR3 anchored 5' RACE. Analysis of dolphin γ gene …

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Pathological Findings In Acute Amitraz Intoxication In Mice, Ayhan Fi̇lazi̇, Tolga Güvenç, Cavi̇t Kum, Seli̇m Sekki̇n Jan 2004

Pathological Findings In Acute Amitraz Intoxication In Mice, Ayhan Fi̇lazi̇, Tolga Güvenç, Cavi̇t Kum, Seli̇m Sekki̇n

Turkish Journal of Veterinary & Animal Sciences

This study examined the pathological changes in tissues and organs with acute intoxications with amitraz and its veterinary medicinal formulation. In total 312 healthy white English mice (Mus musculus), 156 female and 156 male, were used for this purpose. The solutions were given to the mice by gavage at the following dosages: 500, 1000, 1500, 1800, 2200 or 2500 mg of technical amitraz/kg and 50, 100, 250, 500, 750 or 1000 mg of amitraz/kg in a veterinary formulation. There was an interval of 1 week after the administration of the study medication. Necropsies were performed immediately on the mice that …

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Effect Of Culture Medium Volume On The Development And Viability Of Microinjected Or Noninjected One-Cell Hybrid And Cd-1 Strain Mouse Embryos, Haydar Bağiş, Hande Odaman Mercan Jan 2004

Effect Of Culture Medium Volume On The Development And Viability Of Microinjected Or Noninjected One-Cell Hybrid And Cd-1 Strain Mouse Embryos, Haydar Bağiş, Hande Odaman Mercan

Turkish Journal of Veterinary & Animal Sciences

We investigated the effects of drop volume on the development of mouse presumptive zygotes to the completely hatched blastocyst stage. For this aim, we established 3 experimental groups. In the first group, we cultured hybrid presumptive zygotes to the hatched blastocyst stage in 10 presumptive zygotes per 5, 10, 20, 40 and 50 µl drops. In the second group, we used microinjected hybrid zygotes for culture. In the last group, we cultured CD-1 mouse presumptive zygotes to the hatched blastocyst stage. There was no statistical difference in the first group. In contrast, the data demonstrated that there were significant differences …

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A Scanning Electron Microscope Examination Of Heligmosomum Costellatum, Kader Yildiz, Külti̇gi̇n Çavuşoğlu Jan 2004

A Scanning Electron Microscope Examination Of Heligmosomum Costellatum, Kader Yildiz, Külti̇gi̇n Çavuşoğlu

Turkish Journal of Veterinary & Animal Sciences

The morphology of Heligmosomum costellatum, a nematode of field mice (Microtus epiraticus), was described by scanning electron microscope. The scanning electron microscopic view of this nematode revealed that the anterior end was surrounded by 2 cephalic vesicles. The 2 copulatory spicules of the male were enveloped in membrane and the male bursa was large. The female posterior end was characterized by a caudal spine. The body of this parasite had transverse ridges.

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Impaired Antigen Receptor Induced Calcium Mobilization In A Phospholipase C-Gamma1 Deficient B Cell Line., Natarajan Muthusamy, Do Joon Park, Hei-Won Rho, Sue Goo Rhee, Subbarao Bondada Dec 2003

Impaired Antigen Receptor Induced Calcium Mobilization In A Phospholipase C-Gamma1 Deficient B Cell Line., Natarajan Muthusamy, Do Joon Park, Hei-Won Rho, Sue Goo Rhee, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

B lymphocytes express phospholipase C-gamma(1) (PLC-gamma(1)) and phospholipase C-gamma(2) (PLC-gamma(2)) isozymes. However, the relative importance of these two isozymes in B cell signaling is not known. We report here the identification and analysis of a B cell line deficient in PLC-gamma(1). Mature splenic B lymphocytes and a panel of cell lines representing pre-B, immature and mature B cell stages expressed phospholipase C-gamma (PLC-gamma), but not the beta or delta isoforms of phospholipase C (PLC). While all the tested B cell lines and primary splenic B cells expressed PLC-gamma(1) and PLC-gamma(2) isozymes, the L1.2 B cell line exclusively expressed PLC-gamma(2), but …

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The Stromal Cell-Derived Factor-1alpha/Cxcr4 Ligand-Receptor Axis Is Critical For Progenitor Survival And Migration In The Pancreas., Ayse G. Kayali, Kurt Van Gunst, Iain L. Campbell, Aleksandr Stotland, Marcie Kritzik, Guoxun Liu, Malin Flodström-Tullberg, You-Qing Zhang, Nora Sarvetnick Nov 2003

The Stromal Cell-Derived Factor-1alpha/Cxcr4 Ligand-Receptor Axis Is Critical For Progenitor Survival And Migration In The Pancreas., Ayse G. Kayali, Kurt Van Gunst, Iain L. Campbell, Aleksandr Stotland, Marcie Kritzik, Guoxun Liu, Malin Flodström-Tullberg, You-Qing Zhang, Nora Sarvetnick

Journal Articles: Regenerative Medicine

The SDF-1alpha/CXCR4 ligand/chemokine receptor pair is required for appropriate patterning during ontogeny and stimulates the growth and differentiation of critical cell types. Here, we demonstrate SDF-1alpha and CXCR4 expression in fetal pancreas. We have found that SDF-1alpha and its receptor CXCR4 are expressed in islets, also CXCR4 is expressed in and around the proliferating duct epithelium of the regenerating pancreas of the interferon (IFN) gamma-nonobese diabetic mouse. We show that SDF-1alpha stimulates the phosphorylation of Akt, mitogen-activated protein kinase, and Src in pancreatic duct cells. Furthermore, migration assays indicate a stimulatory effect of SDF-1alpha on ductal cell migration. Importantly, blocking …

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Il-23 Produced By Cns-Resident Cells Controls T Cell Encephalitogenicity During The Effector Phase Of Experimental Autoimmune Encephalomyelitis, Burkhard Becher, Brigit G. Durell, Randolph J. Noelle Oct 2003

Il-23 Produced By Cns-Resident Cells Controls T Cell Encephalitogenicity During The Effector Phase Of Experimental Autoimmune Encephalomyelitis, Burkhard Becher, Brigit G. Durell, Randolph J. Noelle

Dartmouth Scholarship

CNS-resident cells, in particular microglia and macrophages, are a source of inflammatory cytokines during inflammation within the CNS. Expression of IL-23, a recently discovered cytokine, has been shown to be critical for the development of experimental autoimmune encephalomyelitis (EAE) in mice. Expression of the p40 subunit of IL-12 and IL-23 by microglia has been shown in situ and in vitro, but direct evidence for a functional significance of p40 expression by CNS cells during an immune response in vivo is still lacking. Here we report that p40 plays a critical role in maintaining encephalitogenicity during the disease course. By using …

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Natural Selection On Protein Polymorphism In The Rodent Genus Peromyscus: Evidence From Interlocus Contrasts, Jay F. Storz, Michael W. Nachman May 2003

Natural Selection On Protein Polymorphism In The Rodent Genus Peromyscus: Evidence From Interlocus Contrasts, Jay F. Storz, Michael W. Nachman

Jay F. Storz Publications

The effects of natural selection are generally locus-specific, whereas migration, drift, and inbreeding are expected to have relatively uniform effects across the entire genome. This suggests that multilocus surveys of multiple populations can be used to distinguish selection from demographic effects. The purpose of this study was to test for evidence of selection on protein polymorphism in natural populations of mice in the genus Peromyscus. We analyzed published data from geographic surveys of allozyme variation and used a coalescent- based simulation model to identify specific loci that deviated from neutral expectations. Observed FST values generally exhibited a remarkably …

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The Nematode Fauna Of Long-Nosed Mice Oxymycterus Spp. From The Bolivian Yungas, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner Apr 2003

The Nematode Fauna Of Long-Nosed Mice Oxymycterus Spp. From The Bolivian Yungas, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

During a long-term survey of the parasites of mammals from all over Bolivia, 44 individuals of Oxymycterus inca and 6 of Oxymycterus paramensis were collected and examined for parasites from the foothills and Eastern Cordillera (Los Yungas) of the Andes of Bolivia. Three species of nematode were found including, from the cecum, a previously unknown genus and species of pinworm, Caroloxyuris boliviensis n. gen, n. sp., aspidoderids representing Nematomystes rodentophilus, and from the stomach, Protospirura numidica criceticola. These helminths occurred in prevalences of 25, 9, and 14%, respectively, in O. paramensis. Caroloxyuris boliviensis resembles species included in …

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Comparison Of Ifat And Elisa Results In Mice Experimentally Infected With Toxocara Canis, Hasan Ayçi̇çek, Mehmet Tanyüksel Jan 2003

Comparison Of Ifat And Elisa Results In Mice Experimentally Infected With Toxocara Canis, Hasan Ayçi̇çek, Mehmet Tanyüksel

Turkish Journal of Veterinary & Animal Sciences

Toxocariasis is a disease which indicates a wide symptomatic spectrum and occurs usually by the ingestion of infective Toxocara canis (rarely Toxocara cati) eggs by humans as a paratenic host. In this study, the sera samples of experimentally infected mice were evaluated on different days by IFAT and ELISA. Regarding the sera samples obtained on the 15^{th} and following days of the study, the groups seropositivity in IFAT by adult and larval sectional antigens and in TES-ELISA were 70, 73.3 and 96.7%, respectively. In the control group, seronegativity in IFAT by adult and larval sectional antigens, and in T. canis …

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Selection For Maternal Behavior In Mice—Direct And Correlated Responses, Chung-Feng Chiang, Rodger K. Johnson, Merlyn K. Nielsen Dec 2002

Selection For Maternal Behavior In Mice—Direct And Correlated Responses, Chung-Feng Chiang, Rodger K. Johnson, Merlyn K. Nielsen

Department of Animal Science: Faculty Publications

Three generations of divergent selection for a maternal care index (MCI) in mice were practiced in each of three replicates. MCI was recorded for 29–32 dams and litters in each replicate/line subclass. Pups of the top (or bottom) 6– 10 dams in each replicate, depending on number of females in the litters, were selected in high (H) and low (L) MCI lines. At birth, litters with more than 10 pups were standardized to 10 pups whereas litters with no more than 10 pups remained intact. On day 4, 8, 12, and 16 of lactation, time budgets during a 30-min session …

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Ccr4-Bearing T Cells Participate In Autoimmune Diabetes., Soon H. Kim, Mary M. Cleary, Howard S. Fox, Icos Coporation, Nora Sarvetnick Dec 2002

Ccr4-Bearing T Cells Participate In Autoimmune Diabetes., Soon H. Kim, Mary M. Cleary, Howard S. Fox, Icos Coporation, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Chemokine receptor expression is exquisitely regulated on T cell subsets during the course of their migration to inflammatory sites. In the present study we demonstrate that CCR4 expression marks a pathogenic population of autoimmune T cells. CCR4 was found exclusively on memory CD4(+) T cells during the progression of disease in NOD mice. Cells expressing the CCR4 ligand TARC (thymus- and activation-regulated chemokine) were detected within infiltrated islets from prediabetic mice. Interestingly, neutralization of macrophage-derived chemokine (MDC) with Ab caused a significant reduction of CCR4-positive T cells within the pancreatic infiltrates and inhibited the development of insulitis and diabetes. Furthermore, …

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The Cd154/Cd40 Interaction Required For Retrovirus-Induced Murine Immunodeficiency Syndrome Is Not Mediated By Upregulation Of The Cd80/Cd86 Costimulatory Molecules, Kathy A. Green, W. James Cook, Arlene H. Sharpe, William R. Green Nov 2002

The Cd154/Cd40 Interaction Required For Retrovirus-Induced Murine Immunodeficiency Syndrome Is Not Mediated By Upregulation Of The Cd80/Cd86 Costimulatory Molecules, Kathy A. Green, W. James Cook, Arlene H. Sharpe, William R. Green

Dartmouth Scholarship

C57BL/6 (B6) mice infected with LP-BM5 retroviruses develop disease, including an immunodeficiency similar to AIDS. This disease, murine AIDS (MAIDS), is inhibited by in vivo anti-CD154 monoclonal antibody treatment. The similar levels of insusceptibility of CD40−/− and CD154−/− B6 mice indicate that CD154/CD40 molecular interactions are required for MAIDS. CD4+ T and B cells, respectively, provide the CD154 and CD40 expression needed for MAIDS induction. Here, the required CD154/CD40 interaction is shown to be independent of CD80 and CD86 expression: CD80/CD86−/− B6 mice develop MAIDS after LP-BM5 infection.

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Histological Evaluation Of Cryopreserved Ovaries In Autologous Abdominal Transplant To NaïVe, Outbred, Cd1, Mice, Wentia Elissa Ford Oct 2002

Histological Evaluation Of Cryopreserved Ovaries In Autologous Abdominal Transplant To NaïVe, Outbred, Cd1, Mice, Wentia Elissa Ford

Biological Sciences Theses & Dissertations

This study showed that fresh and frozen-thawed mouse ovaries would maintain viable primordial or primary follicles post-autologous transplantation. Furthermore, with appropriate hormonal stimulation, these early-stage follicles were shown to develop into Graafian follicles containing a metaphase II antral ovum. Partially sliced ovaries were transplanted into the abdominal cavity of naive, outbred, CD1, white mice. Hormonal stimulation with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) one week after the autologous transplantation stimulated variable degrees of follicle and ovum maturation within the ovarian tissue. There were no major morphological differences observed between the four groups, three treatment and one …

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Maternal Behavior In Mice Selected For Large Litter Size, Chung-Feng Chiang, Rodger . K. Johnson, Merlyn K. Nielsen Sep 2002

Maternal Behavior In Mice Selected For Large Litter Size, Chung-Feng Chiang, Rodger . K. Johnson, Merlyn K. Nielsen

Department of Animal Science: Faculty Publications

A total of 96 lactating mice and their pups from each of three replications of lines selected 21 generations for increased litter size (LS) followed by 23–25 generations of random selection and contemporary unselected control lines (LC) were characterized for maternal behavior. Sixteen dams and their pups from each replication of LS and LC lines were sampled. Litter sizes at birth for LS versus LC in the first, second, and third replicates were 17.3 versus 13.2, 15.9 versus 13.1, and 12.3 versus 10.6 pups, respectively, with LS dams averaging 2.87 ± .70 pups more. One-half of the litters were standardized …

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Defective Cd19-Dependent Signaling In B-1a And B-1b B Lymphocyte Subpopulations., Goutam Sen, Hsin-Jung Wu, Gabriel Bikah, Chandrasekar Venkataraman, Darrell A Robertson, E Charles Snow, Subbarao Bondada Sep 2002

Defective Cd19-Dependent Signaling In B-1a And B-1b B Lymphocyte Subpopulations., Goutam Sen, Hsin-Jung Wu, Gabriel Bikah, Chandrasekar Venkataraman, Darrell A Robertson, E Charles Snow, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Peritoneal and pleural cavities in mice and humans contain a unique population of B-lymphocytes called B-1 cells that are defective in B cell antigen receptor (BCR) signaling but have an increased propensity to produce autoantibodies. Several molecules such as Btk, Vav, and CD19 known to be important for BCR signaling have been shown to be critical for the development of B-1 cells from undefined precursors. Here we demonstrate that B-1 cell unresponsiveness to BCR cross-linking is in part due to defective signaling through CD19, a molecule known to modulate signaling thresholds in B cells. The defective CD19 signaling is manifested …

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Food Storage, Rodents And Hantavirus, Charlotte Brennand Aug 2002

Food Storage, Rodents And Hantavirus, Charlotte Brennand

All Current Publications

No abstract provided.

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Analysis Of The Crude Antigen Of Hymenolepis Nana From Mice By Sds-Page And The Determination Of Specific Antigens In Protein Structure By Western Blotting, Bahadir Gönenç Jan 2002

Analysis Of The Crude Antigen Of Hymenolepis Nana From Mice By Sds-Page And The Determination Of Specific Antigens In Protein Structure By Western Blotting, Bahadir Gönenç

Turkish Journal of Veterinary & Animal Sciences

Protein bands of crude antigens of Hymenolepis nana were determined by SDS-PAGE and Western blotting. Thirty Swiss albino mice were allotted into two groups of 15 each as positive (infected with H. nana) and negative (non-infected with H. nana) groups. The natural infections of H. nana and other helminths were determined by centrifugal flotation of faeces. After bleeding, the mice were necropsied and their guts were examined for H. nana and other intestinal helminths. Sera from mice were tested by Western blotting and the bands obtained from positive and negative groups were compared. The specific protein band for H. nana …

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The Effect Of Trifolium Pratense On Spermatogenesis And Its Acute Toxicity (Ld_{50}) In Mice, Tülay Bakirel, Oya Keleş, H. Hakan Bozkurt, Kemal Ak Jan 2002

The Effect Of Trifolium Pratense On Spermatogenesis And Its Acute Toxicity (Ld_{50}) In Mice, Tülay Bakirel, Oya Keleş, H. Hakan Bozkurt, Kemal Ak

Turkish Journal of Veterinary & Animal Sciences

Trifolium pratense contains phytoestrogens and thus it has estrogenic activity. Estrogen has an important role in the development of the reproductive system and the maintanence of its function, and for this reason the effect of Trifolium pratense on sperm parameters and spermatogenesis was examined. Furthermore, its acute toxicity was established. In this study, the motility and morphology of epididymal spermatozoa taken from animals fed diets contains 20% and 40% Trifolium pratense, were affected. Statistically insignificant differences in the diameter of the seminiferous tubule and the number of primary spermatocytes between the control and experimental groups were observed. Intraperitoneal LD_{50} was …

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Presented Antigen From Damaged Pancreatic Beta Cells Activates Autoreactive T Cells In Virus-Mediated Autoimmune Diabetes., Marc S. Horwitz, Alex Ilic, Cody Fine, Enrique Rodriguez, Nora Sarvetnick Jan 2002

Presented Antigen From Damaged Pancreatic Beta Cells Activates Autoreactive T Cells In Virus-Mediated Autoimmune Diabetes., Marc S. Horwitz, Alex Ilic, Cody Fine, Enrique Rodriguez, Nora Sarvetnick

Journal Articles: Regenerative Medicine

The induction of autoimmunity by viruses has been attributed to numerous mechanisms. In mice, coxsackievirus B4 (CB4) induces insulin-dependent diabetes mellitus (IDDM) resembling the final step of disease progression in humans. The immune response following the viral insult clearly precipitates IDDM. However, the molecular pathway between viral infection and the subsequent activation of T cells specific for islet antigen has not been elucidated. These T cells could become activated through exposure to sequestered antigens released by damaged beta cells, or they could have responded to factors secreted by the inflammatory response itself. To distinguish between these possibilities, we treated mice …

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Visceral Larva Migrans In Mice Caused By Eating Toxocara Canis-Infected Chick Livers, Erkut Tüzer, Müfi̇t Toparlak, Ayşen Gargili, Aynur Gülanber, Vedat Keleş, İlker Efi̇l, Meltem Ulutaş Esatgi̇l Jan 2002

Visceral Larva Migrans In Mice Caused By Eating Toxocara Canis-Infected Chick Livers, Erkut Tüzer, Müfi̇t Toparlak, Ayşen Gargili, Aynur Gülanber, Vedat Keleş, İlker Efi̇l, Meltem Ulutaş Esatgi̇l

Turkish Journal of Veterinary & Animal Sciences

In this study, two subjects, 1) whether Visceral Larva Migrans (VLM) takes place when T. canis larvae-infected chick livers are eaten and 2) whether any differences are present between larval distributions, clinical signs and pathological disorders of the infections caused by eggs and infected liver, were investigated in the chick-mouse model. For this purpose, 15-day-old broiler chicks (n=42) and 3-month-old albino mice (n=62) were used. Thirty chicks were each infected orally with 5000 infective T.canis eggs. These chicks, composed of 6 groups, were necropsied at 2-day intervals and their livers were fed to 42 mice in 6 experimental groups. In …

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Effects Of Alcohol, Passive Smoking And Alcohol Plus Passive Smoking On Some Serum Biochemical Variables In Mice, Mustafa Altinişik, Muharrem Balkaya, Funda Kargin, Hümeyra Ünsal, Cengi̇z Ünsal Jan 2002

Effects Of Alcohol, Passive Smoking And Alcohol Plus Passive Smoking On Some Serum Biochemical Variables In Mice, Mustafa Altinişik, Muharrem Balkaya, Funda Kargin, Hümeyra Ünsal, Cengi̇z Ünsal

Turkish Journal of Veterinary & Animal Sciences

The effects of alcohol, passive smoking and alcohol+passive smoking on certain biochemical variables were investigated in mice serum. For this purpose, 72 female and 49 male Swiss albino mice were used. The animals were approximately 10 weeks old at the beginning of the experiment and their initial body weights ranged from 22 to 31 g. All animals were divided randomly into four groups; the control, alcohol, passive smoking and alcohol+passive smoking groups. Alcohol was given in drinking water at a v/v ratio of 10% during the first week, 20% during the second week and 30% during the following four weeks. …

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The Effects Of An Electromagnetic Field (60-90 Hz And 5 Mt) On Blood Electrolyte Levels In Diurnal Rhythm, Gökhan Eraslan, Mehmet Akdoğan, Ali̇ Bi̇lgi̇li̇, Murat Kanbur, Fatma Şahi̇ndokuyucu Jan 2002

The Effects Of An Electromagnetic Field (60-90 Hz And 5 Mt) On Blood Electrolyte Levels In Diurnal Rhythm, Gökhan Eraslan, Mehmet Akdoğan, Ali̇ Bi̇lgi̇li̇, Murat Kanbur, Fatma Şahi̇ndokuyucu

Turkish Journal of Veterinary & Animal Sciences

In this study, 120 British male mice, weighing 35-40 g, and 5 months old were used. Three groups, one control (Group I) and two experimental (Groups II and III), were formed. One experimental group (Group II) received 60 Hz and 5 mT, while the other (Group III) received 90 Hz frequency and 5 mT magnetic flux density efficacy electromagnetic field (EMF), between 900 and 1700. During this period, blood was taken every hour and blood electrolyte levels (Ca^{++}, P^{+++}, Na^{+}, K^{+}, Cl^{-}) were evaluated. In conclusion, in almost all periods and groups only sodium and calcium levels showed a steady …

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The Toxoplasma Gondii Protein Rop2 Mediates Host Organelle Association With The Parasitophorous Vacuole Membrane, Anthony P. Sinai, Keith A. Joiner Jul 2001

The Toxoplasma Gondii Protein Rop2 Mediates Host Organelle Association With The Parasitophorous Vacuole Membrane, Anthony P. Sinai, Keith A. Joiner

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Toxoplasma gondii replicates within a specialized vacuole surrounded by the parasitophorous vacuole membrane (PVM). The PVM forms intimate interactions with host mitochondria and endoplasmic reticulum (ER) in a process termed PVM–organelle association. In this study we identify a likely mediator of this process, the parasite protein ROP2. ROP2, which is localized to the PVM, is secreted from anterior organelles termed rhoptries during parasite invasion into host cells. The NH2-terminal domain of ROP2 (ROP2hc) within the PVM is exposed to the host cell cytosol, and has characteristics of a mitochondrial targeting signal. In in vitro assays, ROP2hc is …

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The Efffect Of Albendazole And Praziquantel On Strobilocercus Fasciolaris In Experimentally Infected Mice, Kader Yildiz, Ahmet Doğanay Jan 2001

The Efffect Of Albendazole And Praziquantel On Strobilocercus Fasciolaris In Experimentally Infected Mice, Kader Yildiz, Ahmet Doğanay

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to analyze the effects of albendazole and praziquantel on the development and treatment of S. fasciolaris. In order to determine the prophylactic effects on the development of S. fasciolaris, the drugs (albendazole and praziquantel) were given 2 days before the infection and for a period of 30 days. Also, to determine the effects of treatment, both drugs were given on the 63^rd day of the infection and for a period of 14 days. The dosages of albendazole and praziquantel were 15 mg/kg (150 ppm) and 200 mg/kg (2000 ppm) respectively. They were given with the normal …

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Impaired Fast-Spiking, Suppressed Cortical Inhibition, And Increased Susceptibility To Seizures In Mice Lacking Kv3.2 K+ Channel Proteins, David Lau, Eleazar Vega-Saenz De Miera, Diego Contreras, Alan Chow, Richard Paylor, Christopher S. Leonard, Bernardo Rudy Dec 2000

Impaired Fast-Spiking, Suppressed Cortical Inhibition, And Increased Susceptibility To Seizures In Mice Lacking Kv3.2 K+ Channel Proteins, David Lau, Eleazar Vega-Saenz De Miera, Diego Contreras, Alan Chow, Richard Paylor, Christopher S. Leonard, Bernardo Rudy

NYMC Faculty Publications

Voltage-gated K(+) channels of the Kv3 subfamily have unusual electrophysiological properties, including activation at very depolarized voltages (positive to -10 mV) and very fast deactivation rates, suggesting special roles in neuronal excitability. In the brain, Kv3 channels are prominently expressed in select neuronal populations, which include fast-spiking (FS) GABAergic interneurons of the neocortex, hippocampus, and caudate, as well as other high-frequency firing neurons. Although evidence points to a key role in high-frequency firing, a definitive understanding of the function of these channels has been hampered by a lack of selective pharmacological tools. We therefore generated mouse lines in which one …

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Expression And Function Of Hoxc5 And Hoxc6 In The Murine Mammary Gland, Silvia Alejandra Garcia-Gasca Jan 2000

Expression And Function Of Hoxc5 And Hoxc6 In The Murine Mammary Gland, Silvia Alejandra Garcia-Gasca

MUSC Theses and Dissertations

Homeotic genes encode transcription factors, believed to regulate cell proliferation, differentiation, as well as cell migration to create positional identities during embryogenesis. In the mouse, the Hox complex consists of at least 39 genes, organized into four clusters, in four different chromosomes. Hoxc5 and Hoxc6 are adjacent genes located in chromosome 15, and are expressed in the developing somites of the thoracic region and central nervous system with overlapping patterns. Both Hoxc5 and Hoxc6 are also expressed in the mammary gland. This study demonstrates that a targeted disruption of the Hoxc6 gene results in a failure of ductal development in …

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A Defect In Interleukin 12-Induced Activation And Interferon Gamma Secretion Of Peripheral Natural Killer T Cells In Nonobese Diabetic Mice Suggests New Pathogenic Mechanisms For Insulin-Dependent Diabetes Mellitus., Marika Falcone, Brian Yeung, Lee Tucker, Enrique Rodriguez, Nora Sarvetnick Oct 1999

A Defect In Interleukin 12-Induced Activation And Interferon Gamma Secretion Of Peripheral Natural Killer T Cells In Nonobese Diabetic Mice Suggests New Pathogenic Mechanisms For Insulin-Dependent Diabetes Mellitus., Marika Falcone, Brian Yeung, Lee Tucker, Enrique Rodriguez, Nora Sarvetnick

Journal Articles: Regenerative Medicine

The function of natural killer T (NKT) cells in the immune system has yet to be determined. There is some evidence that their defect is associated with autoimmunity, but it is still unclear how they play a role in regulating the pathogenesis of T cell-mediated autoimmune diseases. It was originally proposed that NKT cells could control autoimmunity by shifting the cytokine profile of autoimmune T cells toward a protective T helper 2 cell (Th2) type. However, it is now clear that the major function of NKT cells in the immune system is not related to their interleukin (IL)-4 secretion. In …

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Induction Of Integral Membrane Pam Expression In Att-20 Cells Alters The Storage And Trafficking Of Pomc And Pc1, Giuseppe D. Ciccotosto, Martin R. Schiller, Betty A. Eipper, Richard E. Mains Feb 1999

Induction Of Integral Membrane Pam Expression In Att-20 Cells Alters The Storage And Trafficking Of Pomc And Pc1, Giuseppe D. Ciccotosto, Martin R. Schiller, Betty A. Eipper, Richard E. Mains

Life Sciences Faculty Research

Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides. The bifunctional PAM protein contains an NH2-terminal monooxygenase (PHM) domain followed by a lyase (PAL) domain and a transmembrane domain. The cytosolic tail of PAM interacts with proteins that can affect cytoskeletal organization. A reverse tetracycline-regulated inducible expression system was used to construct an AtT-20 corticotrope cell line capable of inducible PAM-1 expression. Upon induction, cells displayed a time- and dose-dependent increase in enzyme activity, PAM mRNA, and protein. Induction of increased PAM-1 expression produced graded changes in PAM-1 metabolism. Increased expression of …

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