Life Sciences Commons^™

How Do They Do It? Using Omics Approaches To Explore Metabolic Responses Associated With Hypoxia And Exercise Tolerance In The Deepest Diving Pinniped, Elizabeth R. Piotrowski

University of the Pacific Theses and Dissertations

Marine mammals such as northern elephant seals (NES) routinely experience hypoxemia and ischemia-reperfusion events to many tissues during deep dives with no apparent adverse effects. Adaptations to diving include increased antioxidants and elevated oxygen storage capacity associated with high hemoprotein content in blood and muscle. Despite experiencing decreased oxygen tensions during diving, NES likely rely on the mobilization of large lipids stores and catabolism of fatty acids to provide energy to exercising muscle while diving. To identify potential regulatory mechanisms that may underly hypoxia and exercise tolerance in diving mammals, this study used system-wide approaches to characterize changes in genes …

Quantification, Isolation, Characterization, And Biogeography Of Thermoacidophilic Bacteria From Mesophilic Soils, Margaux Karp

Cal Poly Humboldt theses and projects

Many thermoacidophilic bacterial species thrive in hot springs, fumaroles, and geothermally heated soils. These habitats are globally distributed but often small in size and located distantly from similar habitats, and therefore the biogeography of thermoacidophiles may be similar to island biogeography of plants and animals. Little is known about dispersal of thermoacidophiles. In this study I aimed to quantify, isolate, and characterize thermoacidophilic bacteria from non-permissive habitats. I also used genetic approaches to compare these isolates to thermoacidophilic bacteria from permissive habitats to provide insight to their biogeography.

To determine if I could enrich thermoacidophiles from non-permissive habitats, and if …

Effect Of Fenugreek Seeds In Maternal Diet On Some Features Of Newborn Pups In Albino Mice, Karim R. Hamad

Polytechnic Journal

Introduction: Fenugreek seeds have got interest of researchers for their positive effects on laboratory animals and human being, including their effects on reproductive system. The present study aimed to investigate the effects of 5% raw fenugreek seed and its boiled aqueous extract containing diet on adult female mice, for 2 weeks before mating, to evaluate fertility, and some features of newborn pups. Materials and Methods: A total of 90 adult female mice randomly and equally were divided into three groups. Group I: Control, Group II: Female mice treated with 5% raw fenugreek seed containing diet, and Group III: Female mice …

Molecular Characterization Of Proteus Spp. From Patients Admitted To Hospitals In Erbil City, Treska Dh. Kamil, Sanaria F. Jarjes

Polytechnic Journal

The study was carried out to detect the identity of Proteus spp. isolated from various clinical specimens in Erbil City by polymerase chain reaction (PCR) technique. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. Fifty-one Proteus isolates, (47) Proteus mirabilis, and (4) Proteus vulgaris, were undergone PCR assay using specific primers, targeting the genes ureR and Urease C (ureC), that encode for urease enzyme as a virulence factor of Proteus species. It was found that all isolates of Proteus mirabilis yielded positive result for ureR gene with an amplicon length of 225 …

Effect Of Fenugreek Seeds In Maternal Diet On Some Features Of Newborn Pups In Albino Mice, Karim R. Hamad

Polytechnic Journal

Introduction: Fenugreek seeds have got interest of researchers for their positive effects on laboratory animals and human being, including their effects on reproductive system. The present study aimed to investigate the effects of 5% raw fenugreek seed and its boiled aqueous extract containing diet on adult female mice, for 2 weeks before mating, to evaluate fertility, and some features of newborn pups. Materials and Methods: A total of 90 adult female mice randomly and equally were divided into three groups. Group I: Control, Group II: Female mice treated with 5% raw fenugreek seed containing diet, and Group III: Female mice …

Molecular Characterization Of Proteus Spp. From Patients Admitted To Hospitals In Erbil City, Treska Dh. Kamil, Sanaria F. Jarjes

Polytechnic Journal

The study was carried out to detect the identity of Proteus spp. isolated from various clinical specimens in Erbil City by polymerase chain reaction (PCR) technique. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. Fifty-one Proteus isolates, (47) Proteus mirabilis, and (4) Proteus vulgaris, were undergone PCR assay using specific primers, targeting the genes ureR and Urease C (ureC), that encode for urease enzyme as a virulence factor of Proteus species. It was found that all isolates of Proteus mirabilis yielded positive result for ureR gene with an amplicon length of 225 …

Effect Of Fenugreek Seeds In Maternal Diet On Some Features Of Newborn Pups In Albino Mice, Karim R. Hamad

Polytechnic Journal

Introduction: Fenugreek seeds have got interest of researchers for their positive effects on laboratory animals and human being, including their effects on reproductive system. The present study aimed to investigate the effects of 5% raw fenugreek seed and its boiled aqueous extract containing diet on adult female mice, for 2 weeks before mating, to evaluate fertility, and some features of newborn pups. Materials and Methods: A total of 90 adult female mice randomly and equally were divided into three groups. Group I: Control, Group II: Female mice treated with 5% raw fenugreek seed containing diet, and Group III: Female mice …

Molecular Characterization Of Proteus Spp. From Patients Admitted To Hospitals In Erbil City, Treska Dh. Kamil, Sanaria F. Jarjes

Polytechnic Journal

The study was carried out to detect the identity of Proteus spp. isolated from various clinical specimens in Erbil City by polymerase chain reaction (PCR) technique. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. Fifty-one Proteus isolates, (47) Proteus mirabilis, and (4) Proteus vulgaris, were undergone PCR assay using specific primers, targeting the genes ureR and Urease C (ureC), that encode for urease enzyme as a virulence factor of Proteus species. It was found that all isolates of Proteus mirabilis yielded positive result for ureR gene with an amplicon length of 225 …

Effect Of Fenugreek Seeds In Maternal Diet On Some Features Of Newborn Pups In Albino Mice, Karim R. Hamad

Polytechnic Journal

Introduction: Fenugreek seeds have got interest of researchers for their positive effects on laboratory animals and human being, including their effects on reproductive system. The present study aimed to investigate the effects of 5% raw fenugreek seed and its boiled aqueous extract containing diet on adult female mice, for 2 weeks before mating, to evaluate fertility, and some features of newborn pups. Materials and Methods: A total of 90 adult female mice randomly and equally were divided into three groups. Group I: Control, Group II: Female mice treated with 5% raw fenugreek seed containing diet, and Group III: Female mice …

Molecular Characterization Of Proteus Spp. From Patients Admitted To Hospitals In Erbil City, Treska Dh. Kamil, Sanaria F. Jarjes

Polytechnic Journal

The study was carried out to detect the identity of Proteus spp. isolated from various clinical specimens in Erbil City by polymerase chain reaction (PCR) technique. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. Fifty-one Proteus isolates, (47) Proteus mirabilis, and (4) Proteus vulgaris, were undergone PCR assay using specific primers, targeting the genes ureR and Urease C (ureC), that encode for urease enzyme as a virulence factor of Proteus species. It was found that all isolates of Proteus mirabilis yielded positive result for ureR gene with an amplicon length of 225 …

Biol 1411- Botany Laboratory Manual, Yolander R. Youngblood

Open Education Resources

Botany Laboratory Manual By – Yolander R.Youngblood, PhD.

This manual was reviewed by student editors, Ayanna Montegut and Ineceia Carter.

Table of Contents

1. The Plant Cell Lab

2. Mitosis Lab

3. Plant Growth and Development Lab

4. Simple Tissue Lab

5. Leaf Lab

6. Stem Lab

7. Root Lab

8. Evolution of Land Plants Lab

9. Bryophyte Lab

10. Fern Lab

11. Gymnosperm Lab

12. Angiosperm Lab

13. Appendix

a. Writing in the Scientific Notebook

b. How to use the Microscope

c. Plants and Their Structure

d. Additional Video, Lecture, and Lab Resources

Light Pollution Levels On Beaches: How Window Tinting Effects Disorientation Of Sea Turtles On Hilton Head Island, Kathryn (Kate) Hettiger

Biology: Student Scholarship & Creative Works

Loggerhead sea turtles, an endangered species, are decreasing at alarming rates worldwide. Artificial light from beachfront hotels on Hilton Head Island are causing disorientation in Loggerhead sea turtle hatchlings affecting their ability to successfully find the ocean. To mitigate this, exterior windows facing the ocean will be tinted using either tinting spray, which will be implemented at the Marriott Grande Ocean, or using tinting film, which will be implemented at the Marriott Westin. During the typical nesting and hatching season (May-October), sea turtle hatchling tracks will be recorded using drones to compare the effects of various levels of light pollution …

Local Weather Explains Annual Variation In Northern Goshawk Reproduction In The Northern Great Basin, Usa, Allyson B. Bangerter, Eliana R. Heiser, Jay D. Carlisle, Robert A. Miller

Intermountain Bird Observatory Publications and Presentations

Weather is thought to influence raptor reproduction through effects on prey availability, condition of adults, and survival of nests and young; however, there are few long-term studies of the effects of weather on raptor reproduction. We investigated the effects of weather on Northern Goshawk (Accipiter gentilis; henceforth goshawk) breeding rate, productivity, and fledging date in south-central Idaho and northern Utah, USA. Using data from 42 territories where we found evidence of breeding attempts in ≥1 yr from 2011–2019, we analyzed breeding rates using 315 territory–season combinations, analyzed productivity for 134 breeding attempts, and analyzed fledging date for 118 …

Lifelines Fall 2021, Southern Adventist University

Lifelines - Biology Department Newsletter

The Fall 2021 issue of Lifelines contains articles on a former biology student, information on student research, the Palau Adventist Wellness Center, and Department Happenings.

Risk Assessment Of Blackbird Mine, Tinsae Alem

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Blackbird Mine. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Risk Assessment Of Blackbird Mine, Eli Caldwell

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Blackbird Mine. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Risk Assessment Of Pcb Exposure From Mvp In Southwest, Va, Elizabeth Garri

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for PCB exposure from MVP in Southwest Virginia. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Risk Assessment Of Anaconda Copper Mine, Kelly Jarratt

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Anaconda Copper Mine. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Risk Assessment Of Bear Creek Sediments, Rebekah Godfrey

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Bear Creek Sediments. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Risk Assessment Of Yellow Pine Mining Area, Noel Preece

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Yellow Pine Mining Area. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Risk Assessment Of Devil's Swamp Lake, Jocelyn Price

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Devil's Swamp Lake. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Microbial Diversity Of Rural And Urban Soil At Different Depths On Longwood University's Campus, Kristen Dobek, Madalyn Cook

Fall Showcase for Research and Creative Inquiry

Microbial diversity is the range of various kinds of bacteria. The purpose of this experimental research was to determine the complex diversity of bacteria among soil samples at two locations on Longwood University's campus. The study found that there was a higher microbial diversity in the rural soil sample location compared to the urban soil sample location.

The Effect Of Distance From Habitat Fragmentation On Biodiversity Of Invertebrates, Diego Sifuentes, Coleman Taylor

Fall Showcase for Research and Creative Inquiry

Habitat fragmentation happens when human interaction causes an organism's environment to emerge in discontinuities in their habitat. This leads to degradation and disrupts the ecosystem as a whole. This experiment will try to answer the question if habitat fragmentation is still prevalent on invertebrates at a further range. This will be achieved by setting two sites with pitfall traps on both sides on the fragmentation and then a third site will be added at the same distance from the first two sites. It is hypothesized that there will be a less abundance of invertebrates on sites A and B while …

The Process Of Isolating, Purifying, And Analyzing The Gfp Gene From The Pglo Plasmid, And Transforming And Expressing Gfp In E. Coli Cells., Kailyn Westbrook

Fall Showcase for Research and Creative Inquiry

The aim of this project was to isolate, amplify, and purify the GFP gene from the pGLO plasmid, for both analysis as well as the translation and expression into Escherichia coli cell cultures. The Polymerase Chain Reaction (PCR) technique was used to isolate and amplify the GFP gene, which was then purified for use in the agarose gel electrophoresis (AGE) chamber, to distinguish the DNA fragments by size and determine the presence of the desired amplicon. The NanoDrop, SnapGene, and Basic Local Alignment Search Tool (BLAST) softwares were then used for the analysis of the purified amplicon. During the analysis …

Water Clarity Affects Microbial Diversity In Lakes At Longwood University, Maya Smith, Linden Czeizinger

Fall Showcase for Research and Creative Inquiry

This project intends to discover how water clarity affects the diversity of Longwood University's native freshwater microbiomes. The group hypothesized that if the lake water clarity is low, then microbiomes within the lake will be less abundant. The results of this experiment proved that the lake waters were far clearer than previous measurements of clarity had shown, as bacterial colonies were highly abundant.

Micro-Rna, A Solution For Understanding Depression, Marshall White

Fall Showcase for Research and Creative Inquiry

Major depressive disorder is a mood disorder that causes sadness and loss of interest. This affects how one may feel, think, or behave which impact their physical and mental state. People who have been diagnosed with this disorder find themselves losing interest in things they once loved. In the United States there are 16.1 million Americans diagnosed with MDD. This disorder is more common in individuals who have had it in their families which has resulted in the interest in how different genes impact MDD. Micro-RNA has been researched and discovered to have a correlation with MDD. These Micro-RNA's show …

Can Magic Mushrooms Remove Inherited Trauma?, Caleb Edwards

Fall Showcase for Research and Creative Inquiry

This presentation is about a proposal wherein mice are exposed to psilocybin over multiple generations to study the effects of psilocybin on fear response, hippocampal growth and gene expression.

Scanning Electron Microscopic Analysis Of Developmental Speed's Effect On Symmetry In Drosophila Melanogaster, Kyle Hiatt

Fall Showcase for Research and Creative Inquiry

The purpose of this project is primarily to examine the effects of development speed on symmetry in Drosophila melanogaster when employing methylparaben, an anti-microbial preservative, as an additive to the organisms' growth medium. This paraben was selected due to its studied effects on prolonging the length of juvenile development. The research question being examined in this project is whether manipulating developmental speed by lengthening the average developmental cycle would lead to more overall symmetry as opposed to a shorter developmental cycle speed. Baseline assays of juvenile developmental speed of Drosophila melanogaster, both groups with and without methylparaben growth medium, will …

Risk Assessment Of Vaping, Gillian Eshelman

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for vaping. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.

Risk Assessment Of Unity Auto Mart, Ashley Wilson

Fall Showcase for Research and Creative Inquiry

The risk assessment process involves identifying and characterizing hazards, determining dose-response relationships, and assessing possible exposures to toxins in order to inform risk management. The goal of this project was to complete the steps of a risk assessment and develop a perspective on risk management. Data collected from publicly available databases, scholarly articles, and targeted sources were used to perform a risk assessment for Unity Auto Mart. Social, economic, political, and/or legal perspectives were considered when determining the best risk management approaches.