Life Sciences Commons^™

Effects Of Cutting Methods And Drying Temperatures On Hot-Air Drying Kinetics And Quality Of Morchella Esculenta, Lin Fang, Wu Zhaolong, Wu Ying, Ma Lanyu, Liu Zhonglin

Food and Machinery

[Objective] To enhance the added value of Morchella esculenta and optimize its hot -air drying process. [Methods] The effects of cutting methods (whole mushroom, 1/2 cut, and 1/4 cut) and drying temperatures (50,60, and 70 ℃) on drying characteristics, nutritional quality (rehydration ratio, protein content, and total phenolic content ), and antioxidant activity were systematically investigated, and a drying kinetic model was established. [Results] The hot -air drying process of M.esculenta could be divided into two stages:an accelerating drying stage and a falling -rate drying stage. The Page model accurately described the moisture variation during drying (R2=0.980). With increasing cutting …

Research Progress And Prospects Of 5g Technology In The Food Supply Chain, Xie Zifan, Wang Lijuan, Liu Siyuan, Liu Bo, Liu Zhexu, Dong Tieyou

Food and Machinery

As a new generation of mobile communication technology, 5G demonstrates significant potential in the digitalization and intelligent transformation of the food supply chain, owing to its advantages of high data rates, low latency, and massive connectivity. This paper reviews the research progress and application exploration of 5G in the food supply chain in China and abroad, with a focus on typical cases and technical pathways in raw material production, food processing, storage and cold -chain logistics, and quality traceability. Overall, 5G has become an important support for promoting the development of the food supply chain. However, related research and practices …

Optimization Of Fermentation Process And Analysis Of Flavor Compounds In Pitaya-Black Tea Wine, Wei Chunhui, Li Xiaoyi, Yang Yikun, Sang Shixin, Zeng Shiyan

Food and Machinery

[Objective] To develop a composite fermented wine integrating the flavors of pitaya and black tea, optimize the fermentation process, and analyze its volatile flavor compounds. [Methods] The mixture of red -fleshed pitaya juice and black tea infusion is inoculated with yeast for fermentation. Single -factor and orthogonal experiments are employed to optimize the fermentation parameters, and the volatile flavor components are analyzed by headspace solid -phase microextraction coupled with gas chromatography -mass spectrometry (HS-SPME -GC-MS). [Results] The optimal fermentation conditions are determined as follows:a volume ratio of pitaya juice to black tea infusion of 1∶5, an initial sugar degree of …

Effects Of Microwave Irradiation On Major Flavor Compounds In Maotai-Flavor Baijiu With Original Bottled Packaging, Huang Jimin, Qin Haibo, Wu Zhaolong, Zhao Guohui, Liang Jinzhi, Li Bingzheng, Huang Zhimin

Food and Machinery

[Objective] To enhance the quality and market value of original bottled Maotai -flavored Baijiu, this study investigates the effects of microwave irradiation on major flavor compounds during the aging process and optimizes the microwave -assisted aging process. [Methods] Maotai -flavored Baijiu samples were subjected to microwave irradiation, and the effects of irradiation time, irradiation temperature, and power density on the contents of key flavor compounds, including ethyl acetate, ethyl lactate, ethyl butyrate, ethyl hexanoate, acetic acid, and acetaldehyde, were examined in samples stored for 28 days after treatment. [Results] With increasing irradiation time, ester contents first increased and then fluctuated …

Active Ingredients Of Piper Betle Extracts And Their Antibacterial Effects On Levilactobacillus Brevis, Chao-Jung Chen, Hung-Tse Huang, Jung-Feng Hsieh, Bang-Yuan Chen

Journal of Food and Drug Analysis

Levilactobacillus brevis, a common beer spoilage bacterium, causes acidification during brewing. In this study, we aimed to investigate the antibacterial activities of Piper betle extracts against L. brevis and identify their active constituents. A crude ethanol extract was obtained from P. betle leaves and further fractionated into chloroform, ethyl acetate (PBAE), and aqueous extracts via liquid-liquid partitioning. Among all extracts, PBAE exerted the strongest antibacterial effects, forming an inhibition zone (diameter = 57.5 ± 0.71 mm) larger than that formed by the P. betle ethanol extract (diameter = 45.5 ± 0.71 mm) and the gentamicin control (diameter = 21.0 ± …

Nanoparticle-Based Innovations In Lung Cancer Treatment: Challenges And Opportunities, Shailesh Sharma, Chamanpreet Kaur, Gurminder Kaur, Surajpal Verma

Journal of Food and Drug Analysis

Nanoparticles (NPs) present a therapeutic revolution in the context of lung cancer, having several and unique advantages over many traditional drugs, such as increased specificity, minimal side effects on healthy tissues, and increased efficacy. It definitely provides a detailed understanding of preparation techniques for nanoparticles for lung cancer treatment, as well as assessment approaches and therapeutic applications of nanoparticles in cancer treatment. The incorporation of drugs into nanoparticle-based systems is then critically discussed with emphasis on their capability to circumvent MDR and target tumour cell lines. Also, the review mentioned the development in the agnostic application, the nanoparticles that are …

Michigan Academician Volume 50, Issue 2

Michigan Academician

2026 Abstract Issue

Agrivoltaics: Combining Food And Solar Power On Agricultural Lands, Dharla Soteros, Kendall Becker, Spencer J. Good, Ngoni Mufute, Matt Yost, Logan Mitchell, Scott Hotaling

All Current Publications

Utah’s ranchers and farmers face pressure to sell land to developers, conserve water, and manage increasing production costs (Li et al., 2019). One way producers can keep growing food while diversifying and increasing revenue is by integrating agriculture and solar electricity generation on the same land, a practice known as agrivoltaics. In this fact sheet, the authors describe agrivoltaic setup options, summarize agrivoltaics' impacts on livestock and crop production, and discuss implementation economics.

Hagfish Intermediate Filament Proteins: From A Natural Protein System To Functional Biomaterials, Oran Wasserman, Kristin K. Durrant, Katee Stromberg, Brianne E. Bell, Paula E. Oliveira, Justin A. Jones

Biology Student Research

Sourcing protein-based biomaterials directly from nature presents persistent challenges in scalability, processing, and batch-to-batch variability. Recombinant engineering has focused largely on spider silks, and hagfish intermediate filament (HIF) proteins remain a comparatively unexplored alternative, with small non-repetitive subunits that have been produced full-length at high yield. This review examines the biology of naturally produced HIF protein threads from the Pacific hagfish, Eptatretus stoutii, advances in recombinant expression and purification, and their translation into functional biomaterials. We first describe the tripartite domain architecture and biochemical classification of the threonine-rich α- and γ-keratin-like subunits, and then the defensive biomechanics of HIF …

Building Relationships With Family Forest Landowners In The Western Upper Gulf Coastal Plain To Enhance Forest Management And Promote Certification, Sandeepa Pantha, Nana Tian, Ana Gutierrez-Castillo, Joshua Adams, Eric Taylor

Journal of Extension

Responsible forest management provides ecological, economic, and societal benefits. Sustaining these benefits in Arkansas, Louisiana, and Texas depends on the forest management practices of 821,000 family landowners who own 32 million acres of forestland. This study uses an innovative network-building approach to support family forest landowners (FFLs), particularly underrepresented ones, by connecting them with professionals through facilitator teams established in each state by an advisory board. This network-building strategy provides supportive networks for FFLs, fostering trust and connections within a more extensive and diverse FFL community. This network encourages cooperative, communication-centered technical support to FFLs within essential landscapes to enhance …

Top 5 Tree Planting Mistakes, Miles Becker, Daniel Allen

All Current Publications

Planting mistakes can substantially shorten a tree’s life. The consequences of planting mistakes may show up within a year or two or remain hidden until a tree dies inexplicably years later. In many cases, these planting mistakes are easy to avoid, saving the tree owner substantial money and time. Homeowners can use this list of five common planting problems to check the quality of work by hired professionals, as instructions for gardeners or landscapers, or as a quick reminder for more experienced tree planters. By making a few simple changes to tree planting, we will all benefit from longer-lived, larger …

Innovation Ecosystem Model To Achieve Sustainability In Palm-Oil Biofuel Development In Indonesia, Fanny Rahmadhitya, Avanti Fontana, Ignatius Heruwasto

Journal of Environmental Science and Sustainable Development

Achieving sustainable expansion of Indonesia’s palm-oil biofuel sector requires overcoming two interconnected challenges: ecological limits on feedstock expansion and weak translation of research into industry adoption. Although innovation ecosystem research emphasizes multi-actor collaboration, existing innovation ecosystem research provides limited understanding of how governance and innovation intermediation can bridge these gaps in the palm oil biofuel sector. Using a constructivist grounded theory approach, the study draws on 12 semi-structured interviews with key policymakers, industry practitioners, and researchers, analyzed using constructivist grounded theory (CGT) and triangulated with policy documents and a structured comparison with Malaysia. The findings reveal two interconnected bottlenecks. First, …

Why Student Research Matters: A Faculty’S Perspective, Ethan Snow

The Journal of Integrative Physiology

As agreeably conveyed by expert accounts, educational theory, primary literature, program data, and other credible sources, students benefit in countless ways from research experiences. While effort-reward imbalances are often ascribed to mentor-mentee relationships, student research also provides faculty with many benefits; however, these outcomes are often overlooked and comparatively undervalued. The purpose of this article is to highlight why student research matters to faculty. The article describes how mentoring student research can cultivate intellectual growth from cognitive responsibility, professional impact through productivity, and fulfillment in reciprocated mentorship. In serving its purpose, this article may guide faculty investment in student research …

The Lateral Recovery Position In Medical Vs Dental Emergencies, John Regis, Steven Saxe, Jason Ciccotelli, Saskia Richter, Jessica Immonen

The Journal of Integrative Physiology

The lateral recovery position is widely used in prehospital and clinical settings to help maintain airway patency in unconscious patients who are breathing spontaneously. Tracheal anatomy and asymmetries in respiratory structures, along with gravitational effects on soft tissues, may influence the efficacy and safety of this positioning. Clinical nuances related to the setting and secondary health concerns interestingly create disagreement on preferred side for lateral placement. This article explores the anatomical and physiological considerations that support the lateral recovery position—focusing on tracheal asymmetry and its implications for airway patency—along with emergency medical practices in medical versus dental emergencies. It also …

The Effect Of Seedling Age On Nickel (Ni) Accumulation In Maize (Zea Mays L.), Tuba Göçergi̇l, Hava Şeyma İnci̇

Turkish Journal of Agriculture and Forestry

One of the most significant problems facing different ecosystems worldwide is the pollution of soil and water resources with heavy metals, which are harmful to biota. Metals are absorbed by plant roots through soil-plant interaction, leading to accumulation in plants. The plant's growth phase is one of the most important factors in heavy metal uptake and accumulation. Among heavy metal elements, nickel is one of the serious environmental pollutants and plant growth inhibitors due to its toxicity and persistent nature at high concentrations, as well as its bioaccumulation in ecosystems. This study aims to investigate how nickel accumulation in plant …

Species-Specific Metal Uptake Thresholds In Riparian Macrophytes: A Biological Framework For Ecological Risk Assessment, Reyhan Sağlam, Ferhat Gökbulak

Turkish Journal of Agriculture and Forestry

Anthropogenic pressures on freshwater ecosystems have increased metal contamination in riparian zones; however, biological thresholds that distinguish tolerable exposure from ecologically significant accumulation remain poorly defined. This study investigated species-specific thresholds for heavy metal uptake in riparian macrophytes to establish a biologically grounded framework for ecological risk assessment in impacted streams. A gradient encompassing five sites was used to sample four dominant macrophyte species: Typha latifolia, Phragmites australis, Lythrum salicaria, and Persicaria lapathifolia. Concentrations of cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), nickel (Ni), lead (Pb), and zinc (Zn) in aboveground plant biomass were examined in relation to their …

Genetic Variation, Heritability, And Forage Yield Path Analysis Of Iranian Desert Wheat Grass (Agropyron Desertorum Fisch. Ex Link) Genotypes, Reza Mohammadi, Alireza Pourmohammad, Meli̇h Okçu

Turkish Journal of Agriculture and Forestry

Desert wheat grass (Agropyron desertorum Fisch. ex Link) is a valuable species in the temperate zones that exhibits tolerance to cold and drought and naturally grows in arid and semiarid regions of Iran. This research was conducted to investigate the phenotypic diversity of 50 selected genotypes of Iranian A. desertorum based on several agromorphological traits and forage production. Genotypes were planted in a randomized complete block design with four replications in 2016. The results showed highly significant differences among genotypes for all traits (p < 0.01). Broad-sense heritability estimates ranged from low to medium for the evaluated traits. The highest heritability values were observed for crown diameter, plant height (PH) and dry matter yield (DMY), while the genetic advance (GA) values were found for forage fresh weight and DMY. Selection based on traits with high GA and heritability is expected to be effective, indicating sufficient genetic variation for breeding programs. The number of stems (NS) and PH explained more than 73% of the entire variation in DMY in the stepwise multiple linear regression analysis. Principal coordinate analysis (PCoA) revealed that the first three coordinates (PCo1–PCo3) explained 82.4% of the phenotypic variation. According to the PCoA plot based on these coordinates, the genotypes were grouped into two groups. In the factor analysis (FA), factor 1 (FAC1) had a positive relationship with forage yield and NS; therefore, it was identified as a biomass factor. Cluster analysis divided the genotypes into two groups: cluster 1 contained 19 genotypes, and cluster 2 consisted of 31 genotypes. Significant phenotypic diversity was detected among the A. desertorum genotypes, indicating a valuable germplasm to employ in forage varietal improvement programs. Furthermore, the examined genotypes can serve as valuable germplasm for development of a core collection of A. desertorum in Iran.

Integrating Fine-Scale Topography And Stand Structure To Explain Herb-Layer Diversity In Forest Ecosystems, Carla-Maria Aparaschive, Paul Sestras, Alina M. Truta, Irina M. Morar, Catalina Dan, Monica Boscaiu, Florin Ioras, Adriana F. Sestras

Turkish Journal of Agriculture and Forestry

Herb-layer diversity in temperate forests emerges from the interaction between stand structure and fine-scale environmental variation. We studied eight mixed beech–hornbeam and oak–hornbeam plots distributed along a short altitudinal gradient (170–310 m) and contrasting slope aspects in East Europe. For each plot, we measured tree diameter, height and crown-base height, and derived stand structural indices (tree density, basal area, Gini coefficients for DBH diameter at breast height and total height, and median crown base height). Herb-layer communities were characterized using standard diversity indices and Ellenberg indicator values. We then used Jaccard similarity to explore tree–herb cooccurrence and Spearman correlations to …

Strigolactone-Mediated Regulation Of Physiological, Biochemical, And Gene Expression Responses Enhances Salinity Tolerance In Strawberry (Fragaria × Ananassa), Nora M. Al Aboud, Aishah Alatawi, Maryam Maryam, Mi̇kdat Şi̇mşek, Adel A. Rezk, Hossam S. El-Beltagi, Bader Y. Alsubaie, Othman Al-Dossary, Ahmed M. Ismail, Anvar İbrahimov

Turkish Journal of Agriculture and Forestry

Salinity stress is a limiting factor that restricts growth, physiology, and fruit quality of strawberry (Fragaria × ananassa), particularly in irrigated and arid regions across the globe. The current investigation focused on unveiling the role of strigolactone (GR24, 5 µM) in the mitigation of NaCl-induced stress (100 mM) under greenhouse conditions. Salinity stress massively reduced plant performance, including fresh biomass (16.5 g), dry biomass (4.3 g), photosynthetic rate (7.9 μmol CO2 m−2 s−1), and fruit yield (6 fruits per plant), along with a decline in fruit quality (7.2 °Brix). In contrast, foliar application of strigolactone sig-nificantly enhanced the growth and …

A Lightweight Multitask Adaptive Fusion Network With Channel Attention For Uav-Based Crop Detection And Classification, Xuanyu Yue, Zhenyu Wan

Turkish Journal of Agriculture and Forestry

Unmanned aerial vehicles (UAVs) have become essential tools in precision agriculture, facilitating large-scale monitoring of crop health, weed proliferation, and overall field conditions. However, conventional single-task recognition models encounter significant challenges when implemented on resource-constrained UAV platforms, particularly in detecting densely distributed small objects, managing scale variations, and addressing occlusions. To address these challenges, this paper proposes a channel attention-based adaptive fusion network (CNAF-Net) specifically designed for agricultural scenarios. CNAF-Net utilizes MobileNetV3 for hierarchical feature extraction and integrates a channel attention-guided adaptive fusion mechanism that dynamically combines multiscale features across detection, classification, and segmentation tasks. Experiments conducted on the WeedMap …

The Collagen-Derived Tripeptide Gly-Pro-Hyp Suppresses Fgf-5 Expression And Promotes Anagen Induction In C3h/Hej Mice, Yun-Ho Kim, Jin Seok Kim, Woong Yong Kim, Hyun Jung Jo, Zhe Piao, Yeo Cho Yoon

Turkish Journal of Biology

Background/aim: Collagen-derived peptides have shown potential in promoting hair growth, yet their effects on catagen-inducing factors remain unexplored. In this study, the hair growth-promoting effects and molecular mechanisms of Collagen-Tripep20 (CTP), a collagen tripeptide preparation containing 3.2% Gly-Pro-Hyp (GPH), were explored, with a specific focus on fibroblast growth factor 5 (FGF-5) regulation.

Materials and methods: Female C3H/HeJ mice were randomly assigned to four groups (n = 10/group): control, CTP-LOW (400 mg/ kg/day), CTP-HIGH (800 mg/kg/day), and GPH (25.6 mg/kg/day). Following dorsal depilation, test substances were administered dailyvia oral gavage for 7 weeks. Hair regrowth was assessed by visual scoring, histological …

Pifithrin-Α Enhances Ex Vivo Hematopoietic Stem Cell Expansion, Nesli̇han Meri̇ç, Pinar Çolakoğlu Erkan, Fati̇h Kocabaş

Turkish Journal of Biology

Background/aim: Hematopoietic stem cells (HSCs) are essential for treating hematological disorders; however, their low abundance and limited proliferative capacity restrict their clinical use. This study investigated whether pifithrin-α (PFT-α), a small-molecule p53 inhibitor with known genoprotective properties, could enhance the ex vivo expansion of HSCs.

Materials and methods: Mouse lineage-negative (Lin⁻) cells were isolated by magnetic depletion, while human umbilical cord blood (hUCB) and mouse bone marrow (mBM) mononuclear cells were obtained using density gradient centrifugation. CD34⁺ cells were subsequently isolated by fluorescence-activated cell sorting using an antimouse CD34 antibody. The isolated cells were cultured in StemSpan SFEM II (STEMCELL …

Anti-Helicobacter Pylori Activity Of Polycation-Coated Plga Nanoparticles Loaded With Juglone–Silver Complex, Tural Safarov, Emrah Şefi̇k Abamor, Serap Acar, Murat Topuzoğullari, Bükre Kiran Üner, Kübra Karadaş, Seçi̇l Kaya, Zi̇şan Toprak

Turkish Journal of Biology

Background/aim: The rising antibiotic resistance of Helicobacter pylori requires the development of novel treatment approaches. Although natural products (e.g., juglone (5-hydroxy-1,4-naphthoquinone)) and metal antimicrobials (e.g., silver) hold some promise as alternatives, drawbacks like the toxicity and instability of these materials limit their clinical use. Combining juglone and silver with coordination bonds can overcome these drawbacks and yield a more effective antimicrobial complex.

Materials and methods: Juglone–silver (J–Ag) complex was synthesized, encapsulated into PLGA nanoparticles (J–Ag@PLGA), and coated with Eudragit (J–Ag@PLGA+eu). Structural and physicochemical properties of the produced samples were examined using MALDI-TOF MS, FTIR, and UV–Vis spectroscopies; dynamic light scattering; …

Understanding Antimicrobial Peptide Activation In Mesenchymal Stem Cells Through The Aryl Hydrocarbon Receptor Pathway And Inflammatory Signals, Cansu Özdemi̇r Saka, Beyza Turgut, Ceren Tuncay, Cemi̇l Can Eylem, Emi̇rhan Nemutlu

Turkish Journal of Biology

Background/aim: Antimicrobial peptides (AMPs) are small peptides involved in host defense and have been widely studied for their roles in innate immunity against microbial pathogens. Mesenchymal stem cells (MSCs), which provide stromal support in tissues such as bone marrow, are also capable of secreting AMPs, although the signaling pathways controlling this function remain unclear. The present study investigates the role of the aryl hydrocarbon receptor (AHR) signaling, a xenobiotic response pathway, in AMP production by MSCs.

Materials and methods: To assess AMP activation, human MSCs were stimulated with the agonist FICZ and antagonist SR1. TNF-α + IL-1β and LPS were …

Design And Synthesis Of A Novel Benzoxazine-Based Fluorophore With Potential For Lipid Droplet Targeting In Cancer Cells, Eda Açikgöz, Burak Kuzu, Mustafa Çakir

Turkish Journal of Biology

Background/aim: Fluorescence-based visualization of lipid droplets is essential for understanding lipid-related mechanisms in cancer and other metabolic diseases. This study aimed to design and evaluate a novel benzoxazine-based fluorescent probe for selective lipid droplet imaging compatible with multiplexed fluorescence applications.

Materials and methods: A novel benzoxazine-based fluorophore (EA), inspired by the structural features of Nile Red, was synthesized via a two-step strategy. Structural characterization was performed using nuclear magnetic resonance and high-resolution mass spectrometry. Biological performance was assessed in cancer cells under fixed- and live-cell imaging conditions, including cytotoxicity evaluation and compatibility with dual staining.

Results: EA selectively localized to …

Msx2 Is An Erα-Associated Transcription Factor That Sustains Proliferative Capacity And Is Associated With Favorable Prognosis In Luminal Breast Cancer​​, Gamze Turan, Eli̇f Kansiz, Gözde Korkmaz, Hande Özkan

Turkish Journal of Biology

Background/aim: Breast cancer (BC) is the most frequently diagnosed malignancy among women and exhibits substantial molecular heterogeneity. Luminal tumors represent the largest molecular subtype and are primarily driven by estrogen receptor alpha (Erα), ahormone-dependent transcription factor (TF) that regulates gene expression programs controlling proliferation, differentiation, and endocrine response. Herein, the study aimed to identify functionally relevant TFs regulated by ERα as part of a complex regulatory program in luminal BC.

Materials and methods: An integrative transcriptomic approach was applied to identify TFs regulated by ERα in luminal BC. Differential gene expression analysis was integrated with RNA-seq data obtained from estrogen …

Comparison Of Tnfα, Ifnγ, And Dss In Modeling Inflammatory Bowel Disease In Murine Enteroids And Assessment Of The Role Of Zonulin Inhibitor Larazotide Acetate, Sema Nur Gür, Burcu Pervi̇n, Mehmet Emi̇n Şeker, Fati̇ma Aerts Kaya

Turkish Journal of Biology

Background/aim: Inflammatory bowel disease (IBD) is characterized by chronic inflammation of the gastrointestinal tract. We hypothesized that the zonulin inhibitor larazotide acetate (LA), designed to regulate loss of tight junctions between intestinal cells and currently used for the treatment of celiac disease, could also have potential therapeutic value in the treatment of IBD.

Materials and methods: BALB/c enteroids were exposed to dextran sulfate sodium (DSS), tumor necrosis factor-alpha (TNFα), or interferon-gamma (IFNγ) for 48 h to induce an IBD-like environment, followed by treatment with LA. Organoids were assessed for intestinal permeability, viability, and expression of the zonulin (Hp), occludin (Ocln), …

Targeting Mitochondrial And Inflammatory Pathways In Abdominal Aortic Aneurysm: Discovery Of Sigma-1 And Ppar-Γ Agonists Through Plasma Microrna Profiling, Defne Çiğ Kabakcioğlu, İrem Peker Eyüboğlu, Medi̇ Kori̇, Fatma Betül Çeli̇k, Semra Meli̇s Soyer, Mustafa Çalişkan, Can Erzi̇k, Kazim Yalçin Arga, Ceyda Kasavi̇

Turkish Journal of Biology

Background/aim: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease marked by progressive aortic dilation, high rupture-associated mortality, and a complete lack of effective pharmacological therapies. Because microRNAs (miRNAs) are key regulators of vascular inflammation, extracellular matrix remodeling, and smooth muscle cell function, they represent highly promising therapeutic targets. The aim of this study was to employ an integrative systems biology framework to identify novel miRNA-drug regulatory axes for AAA intervention.

Materials and methods: AAA transcriptome data were integrated with experimentally validated miRNA–mRNA interactions to prioritize candidate miRNAs. A total of 22 previously unreported miRNAs were selected for further analysis. …

Resveratrol Modulates Oxidative Stress-Associated Responses And Restores Keratinocyte Migration Following Lps-Induced Inflammatory Injury, Şeyma Dümür, Burcu Bi̇lteki̇n, Hafi̇ze Uzun

Turkish Journal of Biology

Background/aim: Persistent inflammation disrupts keratinocyte migration and contributes to delayed wound healing. Lipopolysaccharide (LPS)-induced inflammatory models are widely used to investigate impaired reepithelialization and inflammatory cellular dysfunction. Resveratrol, a naturally occurring polyphenolic compound, exhibits antiinflammatory and antioxidant properties; however, its therapeutic effects on established inflammatory dysfunction remain insufficiently characterized. This study investigates the effects of resveratrol on LPS-induced keratinocyte dysfunction and compares them with those of boric acid (BA) under inflammatory conditions.

Methods: Human HaCaT keratinocytes were exposed to 10 μg/mL LPS to establish an inflammatory microenvironment. Following LPS pretreatment, the cells were treated with resveratrol (2, 5, or 10 …

Pgcc-Derived Daughter Cells Are Associated With Aggressive Phenotypes In Gastric Cancer​, Özlem Türksoy Terzi̇oğlu

Turkish Journal of Biology

Background/aim: Polyploid giant cancer cells (PGCCs) have been implicated in tumor recurrence, therapeutic resistance, and cellular plasticity; however, the biological properties of PGCC-derived daughter cells (PGCC-DCs) in gastric cancer remain poorly understood. The present study aimed to evaluate the molecular and functional characteristics of these progeny cells in a gastric cancer model.

Methods: The PGCCs were induced in HGC-27 gastric cancer cells using cobalt(II) chlorideCoCl2-mediated hypoxic stress and enriched by size-based filtration. The PGCC-DCs were isolated through single-cell culture and characterized using molecular and functional assays. Clinical assessments of the cellular parameters were conducted by comparing the PGCC-DCs with parental …