Life Sciences Commons^™

Pgcc-Derived Daughter Cells Are Associated With Aggressive Phenotypes In Gastric Cancer​, Özlem Türksoy Terzi̇oğlu

Turkish Journal of Biology

Background/aim: Polyploid giant cancer cells (PGCCs) have been implicated in tumor recurrence, therapeutic resistance, and cellular plasticity; however, the biological properties of PGCC-derived daughter cells (PGCC-DCs) in gastric cancer remain poorly understood. The present study aimed to evaluate the molecular and functional characteristics of these progeny cells in a gastric cancer model.

Methods: The PGCCs were induced in HGC-27 gastric cancer cells using cobalt(II) chlorideCoCl2-mediated hypoxic stress and enriched by size-based filtration. The PGCC-DCs were isolated through single-cell culture and characterized using molecular and functional assays. Clinical assessments of the cellular parameters were conducted by comparing the PGCC-DCs with parental …

Preliminary Investigations Into Measuring Cold Hardiness Of Passiflora Hybrids, Eric Stafne, Haley Williams, Jenny Ryals, Blair Sampson

Publications

Measuring cold hardiness of Passionfruit (Passiflora spp.) is an area of significant interest, especially in new regions where the crop is expanding. However, few studies have explored it in a controlled manner. A previous study by Quesada (1992) revealed that lethal temperatures (LT50) for P. edulis f. flavicarpa were -9 to -10.5°C and -10 to -12.5°C for P. edulis f. edulis. Another species, P. incarnata, was also tested and found to have a cold hardiness level of -11.1 to -13.5°C. In the present study, differential thermal analysis was used on roots of P. incarnata selections from different …

Astaxanthin Elicits A Shared Transcriptional Core And Divergent Cell-Type-Specific Responses In Mcf-7 Breast And Ovcar-3 Ovarian Carcinoma Cells, Duygu Aygüneş Jafari

Turkish Journal of Biology

Background/aim: Astaxanthin, a marine carotenoid, shows antiproliferative activity across cancer models, but its reported mechanisms vary widely by cell type and comparative studies with a common protocol are scarce. This study compared MCF-7 breast (estrogen/ progesterone receptor-positive; wild-type TP53) and OVCAR-3 ovarian carcinoma (high-grade serous; homozygous TP53 R248Q) cells after astaxanthin treatment at cell-line-specific IC50 concentrations.

Materials and methods: Cytotoxicity was measured by WST-1, apoptosis by annexin V/propidium iodide flow cytometry, and 43 cancer-associated genes by a pathway-focused RT-qPCR array (n = 3). Relative expression was calculated by comparative ΔΔCt method with ACTB as the reference gene.

Results: At 72 …

Genetic Inheritance Of Leaf Rolling As An Adaptive Trait For Fog Capture And Drought Tolerance In Wheat, Sabah Merrium, Zulfiqar Ali, Latifa Alhusnain, Muneera D.F. Alkahtani, Sadia Hakeem, Sajid Fiaz, Muhammad Ali Sher, Kotb A. Attia, Seung Hwan Yang, Muhammad Abu Bakar Saddique, Muhammad Hammad Nadeem Tahir

Turkish Journal of Agriculture and Forestry

Wheat is a primary source of sustenance for around 2 billion people worldwide; however, its production is threatened by the water scarcity associated with climate change. In the search for climate-smart wheat varieties, atmospheric moisture in the form of fog and dew is gaining attention as a sustainable, self-irrigating water source for crops. Leaf rolling (LR) is a well-known adaptive trait under water deficit conditions that supports fog moisture capture in wheat; however, little is known about its genetic potential and inheritance pattern, particularly given its high environmental influence. This study assesses the genetic heritability, combining ability, and diversity of …

Valorization Of Buttermilk As A Functional Ingredient In Yogurt Soup: Effects On Physicochemical Properties, Aroma Profile, Amino Acid Composition, And Bioactivities, Emi̇ne Maci̇t, Fatma Zehra Özyilmaz

Turkish Journal of Agriculture and Forestry

​In this study, the feasibility of using buttermilk as a functional ingredient in yogurt soup, a traditional fermented dairy product, was investigated with the aim of valorizing dairy industry by-products. Soup samples prepared with different buttermilk ratios (0%, 25%, 50%, 75%, and 100%) were evaluated for their physicochemical and sensory properties, aroma and amino acid profiles, antioxidant capacity, and antidiabetic activity. The incorporation of buttermilk resulted in statistically significant differences in total solids, ash, and titratable acidity (p < 0.01 and p < 0.05), while no significant changes were observed in fat, protein, or pH values. Increasing buttermilk levels led to higher L* and b* values and lower a* values. Sensory analysis showed that flavor scores increased with buttermilk content, with the highest score observed in the sample containing 100% buttermilk (T5). In the control sample, hexanoic acid, benzoic acid, acetic acid, methyl salicylate, and several ketones were identified as dominant aroma compounds, whereas buttermilk-enriched samples were characterized mainly by hexanoic acid, acetic acid, butanoic acid, ethyl acetate, higher alcohols, and ethanol. Increasing buttermilk content resulted in a higher proportion of alcohols and a reduction in acids, ketones, and aldehydes. Samples with higher buttermilk levels exhibited increased essential amino acid content, while functional amino acids such as γ-aminobutyric acid, β-aminoisobutyric acid, ethanolamine, proline, and alanine reached their highest levels in the T5 sample. The T5 sample showed a DPPH radical scavenging activity of 5.98%, whereas the highest α-amylase inhibition was observed in the control sample. Overall, the results indicate that buttermilk can be effectively used as a functional ingredient in yogurt soup formulations, improving nutritional quality and sensory characteristics.

Tuber Yield Amplification In Dahlia Variabilis L. Under Humic Acid And Fertilizer Combinations, Kedar Mahadev Gheware, Nomita Laishram, Arvinder Singh, Sarabdeep Kour, Sourav Sharma, Aytan Samadova, Melekber Sülüşoğlu Durul, Hali̇l İbrahi̇m Tuzlaci

Turkish Journal of Agriculture and Forestry

Dahlia variabilis L. is a valued tuberous ornamental crop grown worldwide for its attractive flowers. Optimizing nutrient management is crucial for maximizing dahlia growth and tuber yield. In this field study, we evaluated the combined effects of humic acid (HA) and chemical fertilizers on the tuber production of Dahlia variabilis L. A factorial randomized complete block design was used with treatments combining three fertilizer levels (100%, 75%, and 50% of recommended NPK dose) and multiple HA treatments. HA was applied as a soil amendment and foliar spray in combination with inorganic NPK fertilizer. Results showed that integrating HA with reduced …

Bioactive Compounds In Fruits Of Newly Introduced Sweet Cherry Cultivars In Herzegovina, Jasmina Aliman, Mirjana Radović, Ahmed Džubur, Jasna Hasanbegović, Dženan Vukotić, Aleksandra Šupljeglav Jukić, Senad Murtić, Mihajlo Lizdek

Turkish Journal of Agriculture and Forestry

This study evaluated the biochemical characteristics and bioactive compounds of 3 newly introduced Italian sweet cherry cultivars (Sweet Valina, Sweet Stephany, and Sweet Gabriel) grown under the agroecological conditions of Herzegovina (Mostar and Trebinje). The analysis included total soluble solids, total acidity, sweetness index, total phenolic contents, total flavonoid contents, and antioxidant capacity in fruit skin and flesh. Significant differences were observed among cultivars, locations, and their interactions for most analyzed parameters. Fruits from Trebinje generally exhibited higher antioxidant capacity, total phenolic contents in the skin, soluble solids contents, and sweetness index values. Sweet Valina, particularly at the Trebinje location, …

Retraction Notice To “Characterization Of Tfiie-Regulated Genes By Transcriptome Analysis” [Turkish Journal Of Biology, Volume 48, Issue 6, Pages 442–455,Https://Doi.Org/10.55730/1300-0152.2718], Turkish Journal Of Biology

Turkish Journal of Biology

This article has been retracted.

Following concerns raised after publication, the matter was referred to the TÜBİTAK Committee on Research and Publication Ethics (AYEK) for investigation. An Expression of Concern was published while the investigation was ongoing; it was published in the Turkish Journal of Biology, Volume 49, Issue 3, https://journals.tubitak.gov.tr/biology/vol49/iss3/11/.

The investigation has now been concluded. Based on the findings, the project idea and research design underlying the study were used without appropriate attribution or permission. The investigation also determined that a researcher who had contributed to the intellectual foundation of the work—including the development of the …

Towards A Mechanism For Artificial Intelligence Into University Education For Environmental Architecture A Comparative Analytical Study To Evaluate The Impact Of Artificial Intelligence On Self- Learning In Environmental Design., Heba Mahrous Ali Abd El-Aal

Mansoura Engineering Journal

Architectural education is a field that uniquely targets the creativity and continuous development of architecture students. This is especially true within environmental architecture, which relies on diverse spatial and temporal variables that create unique interactions for students, based on the specific concept of environmental design.

Do Soil Amendments Improve Crop Production And Poor Water Infiltration?, Matt Yost, Megan Baker, Tejinder Singh, Grant Cardon

All Current Publications

Soil amendments are widely promoted as tools to improve aggregation, porosity, water holding capacity, nutrient availability, and water infiltration of soils. These benefits are possible, but they depend on the specific soil problem being treated, amendment type and rate, soil chemistry, soil texture and structure, and the time since application. Studies at Utah State University provide a useful short-term test of what growers can reasonably expect from a single surface application of these amendments.

Carbamate Toxicosis In Carnaby’S Cockatoos (Zanda Latirostris) In A Drying Climate, A Le Souef, Z M. Kissane, David Forshaw, K J. Riley, K Payne, T Hawkins, J Coggins, J M. Shephard, R Vaughn-Higgins, K S. Warren

Climate Science Research Articles

Pesticide exposure poses a growing threat to the health of wild avian populations worldwide. This report documents the first detections of agricultural chemicals in the tissues of wild Carnaby’s cockatoos (Zanda latirostris) in Western Australia. The events, characterised by morbidity and mortality, occurred in a national park near Perth, following periods of elevated summer temperatures over two seasons. Toxicological analysis of liver and digestive tract contents identified multiple pesticides and an herbicide, including methomyl, a highly toxic carbamate insecticide. Hot weather conditions preceding the events potentially increased the cockatoos’ use of alternative water sources for drinking, such as those in …

Combined Effects Of Postdistillation Residue Blends From Medicinal Plants On In Vitro Ruminal Microorganisms, Enzymes, Nitrogen And Energy Efficiency, And Antioxidant Status, Mojtaba Akbari Shooshood, Javad Rezaei, Mahdi Ayyari, Yousef Rouzbehan

Turkish Journal of Veterinary & Animal Sciences

In this study, the combined effects of postdistillation residue blends, including pomace, wastewater, and their mixture, derived from rosemary (RO), fennel (FE), spearmint (SP), and rose (ROS), on in vitro microbial populations, enzymatic activities, fermentation, and antioxidant status were evaluated using the gas production technique. The three phytobiotic blends were MP (mixed pomace of RO, FE, SP, and ROS; 1:1:1:1 ratio), MW (mixed wastewater of RO, FE, SP, and ROS; 1:1:1:1 ratio), and MPW (mixed pomace–wastewater of MP and MW; 1:1 ratio). Each blend was used at 2% and 3% of diet dry matter (DM). The experimental diets were as …

Ethylcyanoacrylate Adhesive And Microperforated Polyethylene Tape For The Cutaneous Closure Of Bitches Following Unilateral Total Mastectomy, Leonardo Martins Leal, Fábio Rodrigo Castro Bastos, Joseneia Boeing, Gabriel Aguiar Pavilak, Isaac Romani, Tais Harumi De Castro Sasahara, Paola Castro Moraes

Turkish Journal of Veterinary & Animal Sciences

The use of ethylcyanoacrylate (Super Bonder®) adhesive and microperforated polyethylene tape (Transpore) for the cutaneous closure of extensive wounds following unilateral mastectomy was evaluated, and the healing process was clinically assessed. Included in the study were 15 bitches diagnosed with mammary neoplasia, aged 4–14 years and weighing 3–33 kg. All bitches underwent a 30–60 cm craniocaudal cutaneous incision through the skin in the ventral thoracoabdominal region. Following excision of the tumor and the entire mammary chain, the adjacent tissues were approximated with polyglecaprone 25 sutures. Skin-edge coaptation was achieved through the application of adhesive to half of each wound, while …

Investigation Of Feed Values With In Vitro Technique And Chemical Properties, Particle Size Of Total Mixed Rations Prepared In Commercial Dairy Farms, Özlem Karcioğlu, Betül Zehra Sariçi̇çek

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the chemical and physical properties and feed values of total mixed rations used on dairy cattle farms in Ankara Province. Included in the study were three farms from each of the five districts of Ankara Province (atotal of 15 commercial dairy farms). Total mixed ration (TMR) samples were collected weekly from each farm over a 3-month period in 2025. The chemical composition (crude protein, crude fat, crude fiber, acid detergent fiber (ADF), neutral detergent fiber (NDF), and acid detergent lignin (ADL)), in vitro digestibility parameters (in vitro gas production (IVGP), methane production (MP), organic …

Optimizing Marination Methodology, Holding Temperature, And Holding Time To Upgrade The Physicochemical And Sensorial Attributes Of Japanese Quail (Coturnix Japonica) Breast Fillets, Naveen Uduwawithana, Ruvini Mutucumarana

Turkish Journal of Veterinary & Animal Sciences

The aim was to optimize the effect of meat marination method (unmarinated, tumbling, injection, and immersion), holding temperature (4 oC and 8 oC), and holding time (4 h, 8 h, and 12 h) on the physicochemical and sensorial properties of Japanese quail (Coturnix japonica) breast fillets. Ninety-six deboned breast fillets (mean weight ± SD; 10 g ± 2) from 42-day-old unsexed Japanese quails were marinated. Injection marination showed the highest uptake (p < 0.05). The method × temperature × time interaction (p < 0.05) was significant for cooking yield, meat pH, hardness, cooking loss, and surface color lightness (L*). The highest pH (p < 0.05) was reported when the unmarinated control was retained for 8 h. Tumbling marinated meat when held for 12 h at 8 oC yielded the maximum cooking yield (60.3%). The cooking loss was minimal in unmarinated, tumbled, and immersion-marinated meat when held for 12 h. Irrespective of the temperature, 4-h holding time caused the minimum meat hardness in all four treatments. Tumble-marinated meat when held for 12 h at 8 oC resulted in the minimum cooking loss, hardness, and lightness (L*), as well as the maximum cooking yield. Meat when held at 4 oC for 4 h following injection marination had the highest scores for color, aroma, marinade penetration, flavor, and overall acceptability. In conclusion, quail breast fillets when tumble-marinated and held at 8 oC for 12 h were the best in terms of overall meat quality. Quail breast fillets held for 4 h at 4 oC following injection marination were rated highest by the panelists.

Analytical Biochemistry: A Practical Handbook For Undergraduates, Mark C. Hall

Purdue University Press Books

Methods for characterizing proteins are central to the early stages of drug development and are highly relevant to careers in the pharmaceutical and biotechnology industries. These skills are best learned through hands-on practice in academic laboratory settings. Analytical Biochemistry: A Practical Handbook for Undergraduates presents a laboratory course specifically designed to teach protein analytical techniques to postsecondary students in biochemistry and related fields. Readers are guided through a semester-long research project that mirrors how scientific work is conducted in both academic and industry environments. Instead of focusing on isolated weekly exercises, students work toward a single overarching goal and learn …

Evaluation Of Apoptosis In Peripheral Blood And Milk Immune Cells During Lactation In Dairy Cows With Subclinical Mastitis, Mykola Mykolayovich Zhelavskyi

Turkish Journal of Veterinary & Animal Sciences

The lactation cycle is considered one of the most physiologically demanding periods in dairy cows and is accompanied by substantial immunological remodeling. In the present study, spontaneous apoptosis of lymphocytes, monocytes, and neutrophils was evaluated in clinically healthy Ukrainian Black and White dairy cows (n = 28, aged 3–5 years) during different stages of the lactation cycle: colostrum period (3–5 days), mid‑lactation (90–150 days), drying‑off (5–7 days), and dry period (12–20 days). A further group of 28 cows with subclinical mastitis confirmed by the California Mastitis Test was also included in the study. Peripheral blood and milk samples were collected …

Changes In Mohair Cortisol Concentration And Fiber Growth During Gestation In Angora Goats, Beyzanur Aköz, Erkan Pehli̇van

Turkish Journal of Veterinary & Animal Sciences

The hypothalamic–pituitary–adrenal (HPA) axis plays a central role in regulating long-term stress responses. Keratin-based matrices represent a noninvasive approach for assessing long-term HPA axis activity in farm animals. The present study investigates gestational changes in mohair cortisol concentration and mohair fiber length in Angora goats under routine farm conditions. Clinically healthy does (n = 29; 14 aged 1.5 years and 15 aged 2.5 years) were monitored during the breeding season, and pregnancy was confirmed in 16 does. Mohair samples were collected monthly, fiber length was measured, and cortisol concentrations were quantified using an ELISA. Mohair cortisol concentrations were not significantly …

Floras, Manuals, & Checklists Of The Vascular Plants Of North America: A Bibliography, James P. Smith Jr

Botanical Studies

No abstract provided.

Restructuring Our Agriculture Industry To Reduce The Eutrophication Of The Great Lakes, Kathryn B. Dunn

Tredway Library Prize for First-Year Research

No abstract provided.

Supporting The Adoption Of Digital Technology On-Farm: Ten Tips For Extension, David Rose, Kayleigh Crouch, Lisa Blix Germundsson, Myriam Gaspard, Onno Giller, Livia Ortolani, Robert Strong

Journal of Extension

We held a symposium to exchange lessons from U.S. and European experts on how Extension can be best designed to support adoption of digital agricultural innovation. We highlight 10 tips for an Extension audience in the United States: (a) ensure connectivity between all actors involved in agricultural innovation, (b) utilize the private sector, (c) advocate for adoption costs, (d) shape an enabling digital ecosystem, (e) create a service, not just a technology, (f) update Extension agents' digital skills, (g) support farmer-led innovation, (h) capture everyday farmer practices, (i) monitor and evaluate progress, and (j) offer long-term support.

Valuation Methods And Their Relevance In Achieving Marine Protected Areas Targets: A Systematic Review, Jacob Ankamah, Stephen Hynes, Daragh O'Leary

Journal of Ocean and Coastal Economics

The prolific human-induced pressures on marine ecosystems leading to negative externalities such as beach erosion, biodiversity loss, and climate change are matters of global concern. Marine protected areas (MPAs) have emerged as a tool to conserve marine biodiversity and support human livelihoods. This paper reviews the global research landscape, the evolution of concepts and the relevance of valuation techniques related to the assessment of the contribution of MPAs to societal welfare. The review indicates that less than 3% of the wider MPA literature explores the economic valuation of MPA designation. Of those, there is an obvious preference for non-market-based methods …

Effects Of A 12-Week Tai Chi And Qigong Training Program On Neuromuscular Function And Postural Stability In Elderly Men With Sarcopenia, Muthanna Harith Ekrayyem, Ali Fawzi Ahmed, Saif Rasheed Ghanim

Baltic Journal of Health and Physical Activity

Background: Sarcopenia is associated with progressive declines in neuromuscular function, balance, and increased systemic inflammation in older adults. This study investigated the effects of a 12-week Tai Chi and Qigong training program in elderly Iraqi men with sarcopenia. Materials and Methods: Thirty men aged 60–65 years with sarcopenia were randomly assigned to an intervention group (n = 15) or a control group (n = 15). Neuromuscular performance, postural stability, gait speed, electromyographic amplitude, IGF-1, and CRP-hs were assessed before and after the intervention. Results: The intervention group showed significant improvements in reaction time, electromyographic amplitude, postural stability, gait …

Glycitin Primes Retinal Pigment Epithelium Resilience To High-Glucose Injury Via The Pi3k/Akt Pathway, Shwu-Jiuan Sheu, Yi-Hsiung Lin, Ching-Yu Lin, Chen-Xi He, Chia-Yang Li, Shun-Yuan Tu, Yi-Ching Tsai, Kai-Chun Cheng, Chien-Chih Chiu, Chun-Lin Chen

Journal of Food and Drug Analysis

Diabetic retinopathy (DR) is a leading cause of vision loss, which is driven in part by oxidative stress-induced dysfunction of ocular tissues. We previously showed that high glucose conditions trigger senescence pathways and damage the retinal pigment epithelium (RPE). Here, we identify glycitin, a natural isoflavone, as a candidate cytoprotectant and define its mechanism of action in high glucose-exposed ARPE-19 cells. Using RNA-seq followed by Ingenuity Pathway Analysis, we mapped glycitin-responsive programs and validated key nodes experimentally. Glycitin partially restored cell proliferation, suppressed oxidative stress, and reversed high glucose-induced premature senescence, primarily through modulation of the PI3K/AKT pathway. These findings …

Creative Conversations: The Un-Meeting As An Approach To Agricultural Extension Communication, Jessica R. Spence, Eric K. Kaufman, Megan M. Seibel, Jean A. Parrella, Crystal Tyler-Mackey, Benjamin B. Grove, Bolane Adebayo, Olamide Olowoyo, Sonika Poudel, Stephanie Zhu, Michael Gutter

Journal of Extension

The agricultural industry increasingly faces complex challenges that require diverse stakeholder input, and traditional meeting approaches often limit engagement and perpetuate top-down dynamics that can inhibit effective problem-solving. This article explores the ``un-meeting'' format as an alternative, highlighting its ability to foster inclusive, participant-driven discussions that encourage collaborative brainstorming and actionable outcomes. Based on our experience with a statewide agriculture summit, we discuss the principles underlying un-meetings, the facilitation process, and the practical implications for Extension professionals. Recommendations include the importance of knowledgeable facilitators, a large team for effective management, and limiting session cycles to maintain focus and depth in …

The Weed Flora Of California: A Checklist, James P. Smith Jr

Botanical Studies

No abstract provided.

Building Appreciation For Natural History Collections From Biological Collection Data., Jamie Jensen, Seth Bybee

ScholarsArchive Data

These data represent the pre- and post-survey responses by students in an undergraduate, non-majors biology course given before and after a unit on Natural History Collections. We administered the Revised New Ecological Paradigm (R-NEP; Dunlap et al., 2000) survey which measures pro-environmental attitudes using 15 Likert-scale items.

Vascular Plants With Medicinal Properties, James P. Smith Jr

Botanical Studies

No abstract provided.

A Sneak Peek Into Prehistory: Curating An Interactive Fossil Display To Spark Interest In The Ua Little Rock Natural History Museum, Syd Joheim, Rene Shroat-Lewis

Research and Creative Works Expo

Arkansas does not currently have a dedicated natural history museum, creating a unique opportunity for UA Little Rock following the recent donation of an expansive fossil collection. In response, the university established a natural history museum initiative intended to benefit both the campus community and the broader public across Arkansas. This initiative provides accessible resources for learning about natural science, evolution, and the state’s prehistoric past, areas that are often underrepresented in informal science education. It also serves as a platform for outreach, student engagement, and undergraduate research, strengthening UA Little Rock’s role in science education, workforce development, and community …

Molecular Prevalence And Determinants Of Adenovirus And Norovirus Among Diarrhoeic Children In Ilorin Metropolis, Kwara State, Nigeria, Mutiat Busayo Odebisi-Omokanye, Suleiman Muhammed Mustapha, Mariam Kehinde Sulaiman, Augustine Okechukwu Udeze, Abdulbasit Bolaji Ajibade

Maaen Journal for Medical Sciences

Viral gastroenteritis remains a major public health concern and a leading cause of morbidity among children under five worldwide. Adenovirus (Adv) and Norovirus (Nv) are important etiological agents, yet data on their prevalence and determinants in North-central Nigeria are limited. A cross-sectional study involving 100 children under 5 years with acute diarrhoea was conducted at the Children’s Specialist Hospital, Ilorin, Kwara State between February to December, 2024. Virus detection was performed using qPCR, while a semi-structured questionnaire collected sociodemographic and environmental data. Associations were analyzed using chi-square tests and multivariable logistic regression. Adenovirus prevalence was high (71%), while Norovirus GI …