Supplementation Of Commercial Probiotic Lactobacillus Casei In Rearing Early-Stage Silver Therapon (Leiopotherapon Plumbeus) Larvae: Effects On Growth, Body Composition, And Stress Tolerance, Frolan A. Aya, Reylan C. Gutierrez, Mark Joseph Reyes, Luis Maria B. Garcia

The Philippine Agricultural Scientist

Supplementation of probiotics in live feeds and rearing water may improve the growth, survival, and resistance of fish larvae. This study examined the beneficial effect of a commercial probiotic bacteria Lactobacillus casei on the growth, body composition, and stress resistance in early-stage silver therapon (Leiopotherapon plumbeus) larvae. Larvae at 12 d post-hatch (DPH) (9.76 ± 1.32 mm total length [TL] and 13.48 ± 4.80 mg body weight [BW]) were reared in triplicate groups of 15 larvae per tank from 12 – 40 DPH (Phase 1) and 41 – 96 DPH (Phase 2). From 12 – 40 DPH, larvae were fed …

Production Objectives And Trait Preferences Of Smallholder Farmers In The Philippines: Implications For Designing Breeding Schemes Utilizing Indigenous Swine Genetics, Joy Banayo, Kathlyn Louise Manese, Kaito Furusho, Madeline Kingan, Justine Ayomen, Marcelino Saliw-An, Karina Marie Nicolas, Kayvin Joel Petipit, Dorothy Pagbilao, Vea Roven Arellano, Rene Santiago, Flomella Caguicla, Arnolfo Monleon, Giselle Perlas, Giselle Perlas, Rea Palma Ortego, Sharon Singzon, Agapita Salces, Takahiro Yamagata

The Philippine Agricultural Scientist

Profit maximization is not the only production objective in farming. For smallholder farms worldwide, family sustenance often takes priority, with production being viewed more as savings-oriented than market-oriented. Furthermore, market pressures can lead to the mismanagement of indigenous livestock resources, increasing their risk of genetic erosion. To ensure the long-term conservation of the Philippine native pig, this study aimed to determine the various types of native pig farmers and define the intervention strategies for each type. A total of 432 native pig farmers representing seven provinces in the Philippines (Benguet, Kalinga, Isabela, Nueva Vizcaya, Quezon, Marinduque, and Eastern Samar; n …

Metagenomic Sequencing For Early Detection Of Future Engineered Pandemics: Foreshadowing The Privacy Challenge, Nir Eyal, Bridget Williams, Kevin M. Esvelt, Jane R. Bambauer

UF Law Faculty Publications

In this commentary, we imagine how a global system of metagenomic sequencing could be governed in order to reap the benefits and manage the risks. Global institutional engineers and their academic partners would need to develop a new governance model to ensure that all partners involved in a global metagenomic surveillance system cooperate as needed.

Effect Of Integrated Crop–Livestock Systems On Soil Properties And Microbial Diversity In Soybean Production, Namita Sinha, Brett R. Rushing, Aniruddha Acharya, Shankar Gaapathi Shanmugam

Faculty Publications - Biological Sciences

Integrated crop and livestock systems (ICLSs) have been considered an important management-based decision to improve soil health by carbon sequestration. A two-year study (2019–2021) at CPBES in Newton, MS, was conducted to evaluate the effect of an ICLS on soil microbial diversity in the southeastern region of the USA, representing agroclimatic conditions that are warm and humid. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and characterization of microbial diversity were performed using QIIME2®. Soil fungal diversity pattern showed significant difference (alpha diversity, p = 0.031 in 2020 and beta diversity, p = 0.037 …

The Effect Of Common Road Salts And Organic Additives On Microbial Respiration In Urban Streams, Claire Meara

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

Due to significant population growth and urbanization, there has been an increase in impervious surfaces such as roads and sidewalks. During winter, impervious surfaces are often treated with salt to make road conditions safer. However, this has significant impacts on water quality when salt enters urban streams during runoff events. The addition of road salts to urban streams has been shown to have detrimental effects to various organisms across trophic levels and affects nutrient cycling and greenhouse gas release. These findings have made the development of alternative treatments necessary, such as using organic additives, like beet juice, which can decrease …

Analyzing Food Sources And Food Insecurity Of Kichwa Farming Families In The Ecuadorian Amazon, Reina Watanabe

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

While the right to food is internationally recognized and the United Nations established the sustainable development goal to end hunger and achieve food security by 2030, many households remain without consistent access to food in 2024. In Latin America, approximately 4 in 10 households were reported as food insecure in 2020 with higher rates in rural and indigenous communities. Rural areas, which have great overlap with indigenous communities in Ecuador, experience disproportionately high levels of poverty and food insecurity compared to urban areas. The most populous indigenous nationality in the Ecuadorian Amazon and the participants of this study are the …

Anti-Inflammatory Potential Of Black Cumin Seed Oil And Its Nanoemulsion Formulation Against Lipopolysaccharide-Induced Liver Injury In Mice, Shady Swidan, Hesham Hafez, Rehab Hosni, Hatem Amin

Pharmacy

Original Article

Anti-inflammatory potential of black cumin seed oil and its nanoemulsion formulation against lipopolysaccharide-induced liver injury in mice

Haffez, Hesham^1,2,✉; Hosni, Rehab³; Swidan, Shady A.⁴; Amin, Hatem K.^1,5

Author Information

¹Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, Helwan, 11795, Cairo, Egypt

²Center of Scientific Excellence “Helwan Structural Biology Research”, Helwan University, Helwan, 11795, Cairo, Egypt

³El Sisi Comprehensive Clinic, General Authority for Health Insurance-Giza, Haram, 12556, Giza, Egypt

⁴Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, The British University in Egypt, El-Sherouk, 11837, Cairo, …

Variability In Fruit Quality Traits Of Tropical Australian Jackfruit (Artocarpus Heterophyllus Lam.) Genotypes, Jashanpreet Kaur, Zora Singh, Muhammad Sohail Mazhar, Eben Afrifa-Yamoah, Andrew Woodward

Research outputs 2022 to 2026

Jackfruit exhibits a high degree of genetic diversity due to cross-pollination and heterozygosity; nevertheless, little is known about the characterisation of fruit quality in jackfruit genotypes grown in tropical Australia. In this study, patterns of morphological and biochemical variation in the fruit of 12 jackfruit genotypes were investigated. Maximum fruit weight and pulp weight were recorded in ‘Accession 304 (yellow-fleshed)’, which can be more suitable for domestic markets. ‘Accession 348 (orange-fleshed)’ exhibited the highest fruit edible portion, showing its potential for both table and processing purposes. There was evidence of wide variability among jackfruit genotypes in total dry matter content …

Tumor Expression Of Cd83 Reduces Glioma Progression And Is Associated With Reduced Immunosuppression, Malcolm F Mcdonald, Rachel Naomi Curry, Isabella O'Reilly, Brittney Lozzi, Alexis Cervantes, Zhung-Fu Lee, Anna Rosenbaum, Peihao He, Carrie Mohila, Arif O Harmanci, Akdes Serin Harmanci, Benjamin Deneen, Ganesh Rao

Faculty, Staff and Student Publications

Immunosuppression in malignant glioma remains a barrier to therapeutic development. CD83 overexpression in human and mouse glioma increases survival. CD83+ tumor cells promote signatures related to cytotoxic T cells, enhanced activation of CD8+ T cells, and increased proinflammatory cytokines. These findings suggest that tumor-expressed CD83 could mediate tumor-immune communications.

Root Plasticity Of Selected Corn (Zea Mays L.) Varieties Grown For Food And Forage In Response To Fertigroe®-N And Urea, Vanica Apostol, Edna Aguilar, Lilia Fernando, Lourdes Edano Ma., Tonette Laude, Eureka Teresa Ocampo

The Philippine Agricultural Scientist

Root plasticity, the capacity of a corn root system to modify its growth and development in response to varying environmental conditions, is a crucial adaptability trait for optimizing water and nutrient uptake, especially in a challenging environments. Understanding root plasticity provides insights into the pattern basis of crop adaptation, contributing to the development of improved corn varieties and agricultural practices that optimize the production of both ear shoots and forage. This study assessed root traits and other morphological-physiological responses of three corn varieties (NK8840, IPB Var 6, and Pearl Sweet) grown for food (ear shoots) and forage (baby corn fodder) …

Ecological Interactions Of Cougars, Deer, And Wild Pigs In The Central Coast Range, California, Mitchell A. Parsons

All Graduate Theses and Dissertations, Fall 2023 to Present

Wild pigs are an introduced species in the Americas that negatively affect ecosystems in many ways. Previous research has documented their negative effects on water quality and vegetation and their potential to spread disease among wildlife, domestic animals, and humans. However, how wild pigs affect predator-prey interactions between native wildlife has received little attention. This research explores multiple interactions between wild pigs, a native predator (cougars), and a native prey (black-tailed deer). First, I studies cougar diet to understand how frequently pigs are used as prey. During this project, I also placed wildlife cameras at cougar killed prey to monitor …

Polymeric Sumo-2/3 Chain Signals Are Antagonistically Regulated By The Sumo-Targeted Ubiquitin E3 Ligase Rnf4 And The Sumo-Specific Isopeptidase Senp6, Shaniah A. Irving M.A., Xiang-Dong Zhang Ph.D.

Biology Theses

Polymeric SUMO-2/3 chain modification is critical for chromosome segregation, DNA damage repair, genome stability, stress responses, and protein degradation. However, how these signals are regulated remains poorly understood. Both the SUMO-targeted ubiquitin E3 ligase (STUbL) RNF4 and the SUMO-specific isopeptidase SENP6 recognize poly-SUMO-2/3 chains through multiple SUMO-interacting motifs (SIMs). RNF4 contains four tandem SIMs within a short (~80 amino acids) region, whereas SENP6 possesses eight SIMs spanning a much larger (~500 amino acids) domain.

Our preliminary results show that overexpressing an RNF4 N-terminal fragment containing four tandem SIMs (wild type, WT), but not an inactive SIMs mutant (Mut), increases levels …

Soybean Seed Yield Distribution Within The Canopy As Affected By Nitrogen Supply, Lucia Bonfanti

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Soybean [Glycine max (L.) Merr.] seed yield is influenced by the seasonal availability of resources (i.e., nutrients, light, and water). While nitrogen (N) supply is becoming a soybean yield-limiting factor, the N-induced effect in seed yield components is still unclear. The objective of this research was to assess the effect of N supply on soybean yield components distribution within the canopy. A Full-N treatment, which provided the crop with ample N supply, was compared against a Zero-N treatment in which the crop relied on soil N and biological N fixation at nine high-yielding irrigated soybean environments in Nebraska, with …

Leveraging Within-Field Spatial Variability To Optimize Soybean Yield, Seed Quality, And Profitability, Marina Marly Dalla Betta

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Optimizing yield and seed quality in soybean production is critical for both profitability and meeting market demands. However, within-field variability in soil properties poses a challenge in determining optimal management strategies to maximize soybean yield, seed quality, and profitability. This work aimed to evaluate the spatial variability of soybean fields to optimize soybean seeding rates, seed quality, and profitability. Two case studies were used to carry over this research. The first study looked at the optimal economical site-specific soybean seeding rates using 11 on-farm precision experiments across Nebraska. The second study looked at the protein and oil variability within 22 …

Feeding Habits And Malaria Parasite Infection Of Anopheles Mosquitoes In Selected Agroecological Areas Of Northwestern Ethiopia, Aklilu K. Belay, Abebe Asale, Catherine L. Sole, Abdullahi A. Yusuf, Baldwyn Torto, Clifford M. Mutero, David P. Tchouassi

All Peer-Reviewed Publications

Background: Surveillance of the host–anopheline mosquitoes’ interaction is important for assessing malaria transmission risk and guiding vector control. We assume that changes in malaria vector species’ feeding habits, as well as the surrounding environment, have a substantial impact on varied malaria transmission. In this study, we determined the vertebrate host feeding patterns of anopheline mosquitoes to characterize entomologic risk factors for malaria in Jabi Tehnan, Northwestern Ethiopia. Methods: Blood-fed anophelines surveyed during malaria surveillance in Jabi Tehnan district of northwestern Ethiopia were utilized in this study. They were collected using Centers for Disease Control and Prevention (CDC) light traps deployed …

Effect Of Microsporidia Mb Infection On The Development And Fitness Of Anopheles Arabiensis Under Different Diet Regimes, Godfred Yaw Boanyah, Lizette L. Koekemoer, Jeremy K. Herren, Tullu Bukhari

All Peer-Reviewed Publications

Background: Microsporidia MB (MB) is a naturally occurring symbiont of Anopheles and has recently been identified as having a potential to inhibit the transmission of Plasmodium in mosquitoes. MB intensity is high in mosquito gonads, with no fitness consequences for the mosquito, and is linked to horizontal (sexual) and vertical (transovarial) transmission from one mosquito to another. Maximising MB intensity and transmission is important for maintaining heavily infected mosquito colonies for experiments and ultimately for mosquito releases. We have investigated how diet affects the MB-Anopheles arabiensis symbiosis phenotypes, such as larval development and mortality, adult size and survival, as well …

Neurological Foundations And Technological Interventions For Dyslexia: Advancements And Challenges, Piper Hutson, James Hutson

Faculty Scholarship

Dyslexia, a complex neurological condition affecting reading and language processing, is intricately tied to variations in brain structure and function. This article explores the neurological underpinnings of dyslexia, emphasizing phonological, visual, and attention-related impairments. Furthermore, the analysis highlights various types of dyslexia—phonological, surface, rapid automatized naming, and visual—while addressing their neurological correlations. It also investigates the relationship between dyslexia and other neurodivergent conditions, such as autism and ADHD, focusing on the neurological overlap and differences. To mitigate the challenges associated with dyslexia, advanced technologies such as Virtual Reality (VR) and eye-tracking are discussed for their diagnostic and intervention potential. These …

Examination Of The Relationship Between Algal And Mussel Populations In Streams, Haarika Hebbur

Honors Projects

Due to the ecological importance of both mussel and algal populations, understanding their interactions is critical for further studies in the field. This literature review will explore the functions of both organisms within stream ecosystems, followed by an observational study. The study is designed to evaluate the potential interactions between algal biomass and mussel populations using chlorophyll a concentration as a tool for algal abundance measurement.

2024 Seeded Watermelon Cultivar Evaluation In Indiana, Wenjing Guan

Midwest Vegetable Trial Reports

The annual watermelon cultivar evaluation trial is conducted at Southwest Purdue Agricultural Center (SWPAC) in Vincennes, Indiana. It evaluates yield, fruit quality, and overall plant performance of commercial watermelon cultivars and advanced breeding lines. Purdue Extension and seed companies financially support the trial. The 2024 seeded watermelon cultivar trial evaluated 15 cultivars.

Evaluation Of Pierce’S Disease-Resistant Grapevine Cultivars In Upstate South Carolina, Annie Bruno

All Theses

Interest in wine grape production is increasing in South Carolina, but Pierce’s Disease (PD) caused by the xylem-limited bacteria Xylella fastidiosa (Xf) is a major limiting factor. Xf is spread by sap-sucking insect vectors in the Cicadellidae family such as sharpshooters. In the United States, Xf has become endemic to the southeast as global temperatures rise, causing winter temperatures to increase, which allows the bacteria to survive year-round. However, PD-resistant grapevine hybrid cultivars have become available through breeding V. vinifera genes with native American Vitis species that are resistant to Xf. In this study, the PD-resistant grapevine …

Evaluating The Roles Of Pollinators In Shaping The Floral Color Variation Of Phacelia Dubia, Katherine Riddle

All Theses

Floral color variation within a species is a common occurrence and its maintenance has been a long-standing question in evolutionary biology. Differences in flower color between populations can be shaped by pollinator-mediated selection. Pollinators can exhibit innate and learned preferences for color that may influence floral color variation. These preferences may be molded by the visual environment in which pollinators forage. Most current understanding of how pollinators shape floral color variation involves dramatic differences in dominant pollinators between populations (e.g., hawkmoth vs. bee) and most behavioral work utilized model pollinators like honeybee and bumble bee. However, less is known about …

Informing Management Of American Shad (Alosa Sapidissima) In The Carolinas: Evaluating Novel Fish Passage Approaches And Acoustic Tracking Methods, Margaret Gaither

All Theses

Acoustic telemetry is a valuable tool for fisheries scientists to study the movements and migratory behaviors of aquatic organisms. A critical assumption of this method is that tagged fish exhibit similar behaviors and survival rates as their untagged counterparts. To investigate this, we conducted a 12-week laboratory study on adult American Shad (Alosa sapidissima) using gastroesophageal insertion of acoustic transmitters. We implanted 80 fish with dummy transmitters and monitored tag retention and survival. Our analysis, employing a mixed effects Cox’s proportional hazards model, revealed no significant differences in survival between tagged and control fish, regardless of sex. Additionally, …

The Effect Of Packaging On The Acceptance Of Natural Peanut Butter, Perian M. Reese

All Theses

The aim was to determine the effect of packaging on the acceptance of natural peanut butter, especially concerning the acceptance of the undesirable oil layer. The jars of peanut butter differed by color, label size, and inclusion of qualifying statements. 119 participants across two groups completed this study. Both groups completed an eye-tracking study in a mock grocery setting. The first group rated all five jars in appearance, expected quality, and overall rating. They were given one sample cup of peanut butter and gave hedonic liking values for color, oiliness, peanut flavor, smoothness, sweetness, and overall liking. The second group …

On Humans, Hogs, And Habitat: How Landscape Features And Vegetation Influence Wild Hog (Sus Scrofa) Detection On Private Lands And Using Existing Wild Hog Outreach Websites To Create An Effective Website For South Carolina, Andrew Jamison

All Theses

Invasive species are a source of human conflict with wildlife on a global scale and exist to the detriment of native wildlife species. Invasive species often possess the ability to respond rapidly to change and they often exist alongside humans. Wild hogs (Sus scrofa) are perhaps one of the most infamous examples of an invasive species. The spread of wild hogs necessitates effective communication with the public being impacted by them, as well as a continuation of research to learn more about wild hog behavior to promote effective management. Chapter 1 focuses on how we created a website …

Investigation Of Chondrogenesis, Myogenesis, And Osteogenesis After 2,4-Dtbp, 2-Bp, 4-Bp, 3,5-Dtbc Exposure In Zebrafish, Haley Brashears

All Theses

Synthetic phenolic antioxidants (SPAs) are used in personal care products, food packaging, and water pipes, common SPAs include 2,4-di-tert-butylphenol (2,4-DTBP), 2-tert-butyl phenol (2-BP), and 4-tert-butyl phenol (4-BP). These compounds are detected in the environment and in human biological samples. 2,4-DTBP has been detected in cord plasma, placenta, and breast milk samples. This drives the concern regarding developmental toxicity. In human iPS cells 2,4-DTBP impairs differentiation into somites and osteoblasts. Other SPAs, like 2,6-di-tert-butyl-hydroxytoluene caused increased rates of malformations including yolk and pericardial edema, and spinal curvature in 5-day post fertilization (dpf) zebrafish. …

Developing Holistic And Reduced Fertilization Guidelines For Southeastern Peach (Prunus Persica (L.) Batsch) Orchards, Richardson Bien-Aime

All Theses

Peach production in the southeastern United States requires optimizing fertilization practices to balance economic sustainability and environmental health. Current guidelines may overlook rootstock-specific nutrient requirements and fail to align with orchard-specific conditions, often resulting in overfertilization, increased costs, and environmental risks. This study, conducted at Clemson University’s Musser Fruit Research Center during 2023 and 2024, aimed to refine fertilization practices through two complementary experiments. The first experiment investigated how three rootstocks, ’MP-29’, Lovell, and Guardian, influence nutrient dynamics of ‘Carored’ peach trees. Nutrient concentrations were measured in various tree organs, including leaves, pruned wood, thinned fruitlets, and harvested fruits, along …

A Drosophila Model Of Mucopolysacchridosis Iiia, Rebecca Bishop

All Theses

Mucopolysaccharidosis IIIA (MPS IIIA) is a rare lysosomal storage disorder that arises from inability to break down heparan sulfate (HS) because of mutations in the N-sulfoglucosamine sulfohydrolase (SGSH) gene. We used a deletion mutant of the Drosophila melanogaster Sgsh gene along with three point mutations analogous to mutations observed in patients (S64W, L89P, S301P) to show an increase in the average percent of area with lysosomal puncta in the fly brains of our mutants using Lysotracker. RNA sequencing of brains of mutant and control flies showed 441 (Knockout), 337 (S64W), 155 (L89P), and 96 (S301P) differentially expressed …

Lateral Dispersal Of Adult Trichoptera: Flight Distance And Traveling Pattern, Peilin Tan

All Theses

Trichoptera species (caddisfly) are usually used as bio-indictors for surface water quality due to their sensitivity to environmental changes, and if assessing water quality with light-trapped adults, knowing their travelling patterns will improve the accuracy of such assessments. To investigate how far a caddisfly will migrate from their emerging stream, three streams in South Carolina with different widths (Wildcat Creek 1.2 m, Brasstown Creek 11.2 m and Chattooga River 26.95 m) were selected for this study with light traps placed at three distances (0 m, 50 m, 100 m) from the streams. How long and when? Both species richness and …

2024 Standard-Sized Seedless Watermelon Cultivar Evaluation In Indiana, Wenjing Guan

Midwest Vegetable Trial Reports

No abstract provided.

Primary Industries Education Foundation Australia (Piefa) Storm And Flood Industry Recovery Program: Evaluation Report, Tamara Van Der Zant, Katherine Dix

Program monitoring and evaluation

Despite the growing diversity of careers in Primary Industries, young peoples’ pursuit of agricultural careers is often hampered by outdated perceptions of the sector and its opportunities. As Primary Industries adapt to the threat of natural disasters by turning to scientific and technological solutions, a diverse, highly skilled workforce will be required. In the wake of the devastating impacts of storm and floods in early 2021 across New South Wales, the Storm and Flood Industry Recovery Program (SFIRP): Creating resilience through empowering school curriculums about primary industries careers project was developed. To aid the long-term recovery and resilience of the …