Life Sciences Commons^™

Long-Term Climatic Stability Drives Accumulation And Maintenance Of Divergent Freshwater Fish Lineages In A Temperate Biodiversity Hotspot, Sean James Buckley, Chris J. Brauer, Peter J. Unmack, Michael P. Hammer, Mark Adams, Stephen J. Beatty, David L. Morgan, Luciano B. Beheregaray

Research outputs 2022 to 2026

Anthropogenic climate change is forecast to drive regional climate disruption and instability across the globe. These impacts are likely to be exacerbated within biodiversity hotspots, both due to the greater potential for species loss but also to the possibility that endemic lineages might not have experienced significant climatic variation in the past, limiting their evolutionary potential to respond to rapid climate change. We assessed the role of climatic stability on the accumulation and persistence of lineages in an obligate freshwater fish group endemic to the southwest Western Australia (SWWA) biodiversity hotspot. Using 19,426 genomic (ddRAD-seq) markers and species distribution modelling, …

The Allergen Profile Of Two Edible Insect Species—Acheta Domesticus And Hermetia Illucens, Shaymaviswanathan Karnaneedi, Elecia B. Johnston, Utpal Bose, Angéla Juhász, James A. Broadbent, Thimo Ruethers, Emily M. Jerry, Sandip D. Kamath, Vachiranee Limviphuvadh, Sally Stockwell, Keren Byrne, Dean Clarke, Michelle L. Colgrave, Sebastian Maurer-Stroh, Andreas L. Lopata

Research outputs 2022 to 2026

Scope: Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need to feed the growing population. Tropomyosin is the main insect allergen; however, additional potential allergens are not well characterized and the impact of extraction procedures on immunological reactivity is unknown. Methods and results: Proteins from different commercial food products derived from cricket (Acheta domesticus) and black soldier fly (BSF) (Hermetia illucens) are extracted using five different extraction buffers. The proteins are analyzed by SDS-PAGE and immunoblotting using allergen-specific antibodies and crustacean allergic patient sera. IgE binding bands are analyzed by mass spectrometry …

Conceptualising Project Environmental Impact Assessment For Enhancement: No Net Loss, Net Gain, Offsetting And Nature Positive, Angus Morrison-Saunders, Luis Sánchez

Research outputs 2022 to 2026

With declining biodiversity worldwide, the need for development proposals to deliver positive, not negative, environmental outcomes is clear. Arguably, this is a long-standing goal of environmental impact assessment (EIA). This article explores the extent to which current project EIA practice can achieve enhancement, no net loss or a net gain of biodiversity and Nature Positive goals. Using reflexivity, a conceptual approach is applied to policies and examples from practice. The environmental enhancement goal of EIA is explained. The mitigation hierarchy is applied to a hypothetical mining example for accomplishing no net loss/net gain of biodiversity through offsets. Nature Positive is …

Effect Of High-Pressure Processing On The Antioxidant And Antibacterial Properties Of Honey, Ayesha Faraz, Mark Williams, Wmad Binosha Fernando, Vijay Jayasena

Research outputs 2022 to 2026

High pressure processing (HPP) have the potential to be used as an alternative to commercial thermal processing of honey. Its impact on the antioxidant and antibacterial properties of honey have yet to be understood properly. This study focuses on the effects of HPP on the antioxidant and antibacterial properties of honey. Six different honey samples were subjected to HPP at 400 and 600 MPa for 10 and 15 min. The antioxidant properties were measured in terms of total phenolic content, total flavonoid content, the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) free radical scavenging assay. The antibacterial activity …

Diel Movement Patterns In Nominally Nocturnal Coral Reef Fishes (Haemulidae And Lutjanidae): Intra Vs. Interspecific Variation, William P. Collins, David R. Bellwood, Renato A. Morais, Nathan J. Waltham, Alexandre C. Siqueira

Research outputs 2022 to 2026

Movement of animals, habitat connectivity, and nutrient transport are key elements in the ecological functioning of coral reef ecosystems. However, most studies have focused on the diurnal component of these aspects; nocturnal patterns are less known. Our study aims to address this knowledge gap by assessing diel movement patterns in ecologically and commercially important nocturnal coral reef fishes. We evaluate 122-days of acoustic telemetry data from 4 nocturnal species in 2 coral reef fish families (Haemulidae and Lutjanidae) on the Great Barrier Reef. For all species, we found a clear division between daytime resting areas on the reef and a …

Exact Testing For Heteroscedasticity In A Two-Way Layout In Variety Frost Trials When Incorporating A Covariate, Angelika A. Pilkington, Brenton R. Clarke, Dean A. Diepeveen

Grain and Other Field Crops Research Articles

Two-way layouts are common in grain industry research where it is often the case that there are one or more covariates. It is widely recognised that when estimating fixed effect parameters, one should also examine for possible extra error variance structure. An exact test for heteroscedasticity, when there is a covariate, is illustrated for a data set from frost trials in Western Australia. While the general algebra for the test is known, albeit in past literature, there are computational aspects of implementing the test for the two way when there are covariates. In this scenario the test is shown to …

Should Crop Sequences In Western Australia Include More Lupins?, Christophe D'Abbadie, Sud Kharel, Ross Kingwell, Amir Abadi Ghadim

Grain and Other Field Crops Research Articles

Reducing greenhouse gas emissions is an increasing priority for Australian grain producers. Could substituting lupins for canola, as a rotational break crop, enable farmers to reduce their emissions by less use of nitrogenous fertilisers?

Aim

This study aims to identify if replacing canola with lupins in rotations at a range of locations in Western Australia’s grainbelt is environmentally and economically attractive.

Methods.

Bio-economic simulation modelling is used to examine the gross margins and emissions associated with replacement of canola by lupins at 14 locations in the grainbelt of Western Australia in variousland use sequences.

Key results

Replacing canola with lupins …

The Potential Effects Of Abiotic Stresses On Ziziphus And Acacia Species Grown Under Uae Climate Condition, Almaha Abdulla Alneyadi

Theses

This comprehensive thesis delves into a comparative analysis of two prominent desert plant species, namely Acacia and Ziziphus, shedding light on their distinct adaptive features in response to challenging environmental conditions. The exploration spans various facets, including the germination process, tap root development, and their reactions to water stress, ultimately revealing the superior hardiness and adaptability of Acacia over Ziziphus. In the realm of germination, Acacia stands out with its swift and efficient initiation, indicating a robust mechanism for establishing its presence in arid landscapes. The examination of tap root development further underscores the resilience of Acacia, as it demonstrates …

Study On The Chemical Composition And Antioxidant Activities Of Essential Oil Extracted From Horwoodia Dicksoniae Growing In The Uae, Salama Sari Al Mansoori

Theses

Plant-derived substances including essential oils, secondary metabolites and other chemical substances are gaining attention due to their broad spectrum use in different fields. Native medicinal plants can play a key role in the healthcare field in any country. Horwoodia dicksoniae is a native endangered plant in UAE. The current study is about the chemical composition and antioxidant properties of H. dicksoniae’s essential oil as well as the evaluation of growth of this native endangered plant under different environmental conditions. For this purpose, the first step included confirmation of the viability of seeds obtained from a seed bank following the …

Ecu-Mss-2 Dataset, A New Multi-Species Seagrass, Md K. Noman, Syed M. S. Islam, Seyed M. Jalali, Jumana Abu-Khalaf, Paul Lavery

Research Datasets

The "ECU-MSS-2' dataset contains four different habitats, 'Amphibolis' spp (hereafter 'Amphibolis"), 'Halophila' spp (hereafter 'Halophila'), 'Posidonia' spp (hereafter 'Posidonia') and 'Background'. We compiled this image dataset from different sources. The 'Halophila' images were collected by the Centre for Marine Ecosystems Research, Edith Cowan University, Western Australia.

The 'Amphibolis' 'Background' and 'Posidonia' images were collected by the Department of Biodiversity, Conservation and Attractions (DBCA), Australia. The 'Amphibolis' class includes the seagrass species Amphibolis griffithii and Amphibolis Antarctica. The 'Halophila' class includes Halophila ovalis, while the 'Posidonia' class includes Posidonia sinuosa, Posidonia coriacea, and Posidonia Australis. The 'Background' class includes coral, sand, …

Invasive Wild Pig (Sus Scrofa) Diets On Barrier Islands In The Southeastern United States, Vienna R. Canright, Antoinette J. Piaggio, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Background

Biological invasions are a leading cause of reductions in global biodiversity. Islands are particularly sensitive to invasions, which often result in cascading impacts throughout island communities. Wild pigs (Sus scrofa) are globally invasive and pose threats to numerous taxa and ecosystems, particularly for islands where they have contributed to declines of many endemic species. However, the impacts of wild pig diet on the flora and fauna remain understudied in many island systems.

Results

We used DNA metabarcoding of wild pig fecal samples to quantify the seasonal diet composition of wild pigs on three barrier islands in the …

Characterizing Feral Swine Movement Across The Contiguous United States Using Neural Networks And Genetic Data, Rachael M. Giglio, Courtney F. Bowden, Ryan K. Brook, Antoinette J. Piaggio, Timothy J. Smyser

United States Department of Agriculture Wildlife Services: Staff Publications

Globalization has led to the frequent movement of species out of their native habitat. Some of these species become highly invasive and capable of profoundly altering invaded ecosystems. Feral swine (Sus scrofa × domesticus) are recognized as being among the most destructive invasive species, with populations established on all continents except Antarctica. Within the United States (US), feral swine are responsible for extensive crop damage, the destruction of native ecosystems, and the spread of disease. Purposeful human-mediated movement of feral swine has contributed to their rapid range expansion over the past 30 years. Patterns of deliberate introduction of …

Discovery Of A Novel Invasive Rodent Species On Wake Atoll With A Desert Southwest Usa Origin, Antoinette J. Piaggio, Eric Lanners, Daniel R. Taylor, Aaron B. Shiels, Marjorie Matocq, John Eisemann

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive species, particularly omnivorous rodents, have damaged island ecosystems around the world. The unique flora and fauna of island systems have typically not evolved with omnivorous rodents and are heavily impacted by their introduction. Wake Atoll of the southern Pacific Ocean is a 707-hectare coral island complex of three islands managed by the United States Department of Defense (DoD). DoD restricts civilian access to the atoll but aircraft and seacraft regularly visit the island to transport military equipment and to sustain the year-round military and contractor personnel stationed on the atoll. Wake Atoll had been invaded by Rattus rattus complex …

Unsung Heroes In Conservation: Evaluating The Limitations Faced By New England Wildlife Rehabilitation Centers And Proposing Solutions For Their Support And Recognition, Shaylee M. Sarmiento

Honors Theses and Capstones

Wildlife rehabilitation is an often-unacknowledged practice within the overarching field of wildlife protection and conservation. This practice involves the treatment and hopeful release of wild animals affected by various ailments. With the continued expansion of and interest in the field, it could be assumed that wildlife rehabilitation is supported and funded by government bodies, but this is not the case. Because of the lack of funding and resources, many wildlife rehabilitation centers cannot achieve their full rehabilitative potential and expand their reach in the conservation world. Additionally, an absence of support leads critics to question the ethical standards of wildlife …

Comparing Calcium, Vitamin D, And Calorie Intakes Among Unh Army Rotc Cadets With Low And Normal Bone Mineral Status, Ava Gaudette

Honors Theses and Capstones

No abstract provided.

Assessment Of Open Top Chambers To Simulate Effects Of Climate Change On Soil Temperature And Cover Crop Response In Agricultural Systems, Gretchen Bahmueller

Honors Theses and Capstones

Climate change is expected to yield warmer winters that have the potential to place additional stress on our already stressed agricultural systems. Understanding how agricultural systems may respond to these changes is essential to creating crop and land management plans that ensure food security for future generations. To better understand how warming winters can/will affect air and soil temperatures and cover crop performance, open top chambers (OTCs) were deployed post cover crop seeding in a field experiment at the UNH Kingman Research Farm in Madbury, NH. The experiment consisted of four cover crop treatments sown into or after corn: an …

Density Dependent Calling Behavior In North Pacific Humpback Whales, Lucas J. Anderson

Honors Theses and Capstones

Animal density can define factors such as mating success, food acquisition, and communication. Inferring animal density from vocal rates is an important tool for estimating abundance and space use in difficult-to-study species like marine mammals and requires an understanding of the relationship between social context and calling rates. The goal of this project was to investigate the relationship between animal density and call rate in Southeast Alaskan humpback whales (Megaptera novaeangliae). Passive acoustic monitoring data were collected along with visual observations gathered near Frederick Sound and Stephen’s Passage, Southeast Alaska, over two summers (2019, 2022). Observers watched whales …

Utilizing Plasmid Dna Hydrogels For Cell-Free Protein Production, Tinotenda Duche

Honors Theses and Capstones

Proteins play an important role in various biological processes and are crucial components in biomedical research and therapeutics. Cell-free protein synthesis (CFPS) has provided a valuable approach for rapid protein production outside living cells, involving both transcription and translation processes. However, traditional CFPS methods encounter challenges such as high cost, relatively low yields, and scalability. The end goal of our project is to incorporate our template into the hydrogel. In this work, we report advancements towards that by trying to optimize our solution of CFPS. Unfortunately, the standards for pDNA hydrogel synthesis are not met by the pDNA production and …

Alpaca Assisted Activities In The New England Alpaca Industry: A Possible Solution To Farm Profitability And Participant Mental Health, Ashley Michele Masse

Honors Theses and Capstones

The high operating costs of the New England alpaca industry were putting the majority of alpaca farmers in this region in a state of financial loss. On a separate note, American communities were experiencing a prevalence of anxiety and depression. What if a single solution could help improve the outcome of both these challenges that seem to have little to do with each other? Alpaca Animal Assisted Activities (alpaca AAAs) was a possible solution. This study explored New England alpaca farm profitability and alpaca AAAs in a way no other research study has done before. A Farmer Survey was mailed …

Equine Rescue Success In The United States: A Statistical Analysis, Carly J. Cave

Honors Theses and Capstones

The high inflation that has characterized the post-pandemic period in the United States has increased the costs of horse ownership for private owners and equine rescue organizations, which are facing critical pressures. Over 200,000 equines are at risk for neglect or slaughter in the United States each year, which far exceeds the capacity to house these equines at rescue organizations. While demand on these organizations is high, their ability to afford appropriate resources for their care is insufficient. This study examined geographical and economic factors that impact equine rescues’ success in taking in at-risk equines and adopting them to forever …

Alpaca Assisted Activities In The New England Alpaca Industry: A Possible Solution To Farm Profitability And Participant Mental Health, Ashley Michele Masse

Honors Theses and Capstones

The high operating costs of the New England alpaca industry were putting the majority of alpaca farmers in this region in a state of financial loss. On a separate note, American communities were experiencing a prevalence of anxiety and depression. What if a single solution could help improve the outcome of both these challenges that seem to have little to do with each other? Alpaca Animal Assisted Activities (alpaca AAAs) was a possible solution. This study explored New England alpaca farm profitability and alpaca AAAs in a way no other research study has done before. A Farmer Survey was mailed …

Distribution Of Tick Species On Free-Ranging Ungulates In Northern New England, Karisa Graham

Honors Theses and Capstones

In Northern New England, ungulates are often parasitized by ticks, which is one of the leading causes for the decline in population. Ungulates are a good host for ticks, specifically deer ticks and winter ticks, and these ticks cause many tick-borne diseases in humans as well. The purpose of this study was to assess passive sampling from harvested animals as a means of tick surveillance in Vermont and New Hampshire. Ticks were collected from deer and moose and mapped throughout the two states by ArcGIS to visualize the trends in distribution. Relative abundance was greater in southeastern Vermont, with no …

Investigation Of The Effects Of Ocean Acidification On Juvenile Homarus Americanus Feeding Behavior, Sophia Roy

Honors Theses and Capstones

Climate change has led to increased CO2 absorption by ocean surface waters, resulting in decreased pH levels, a phenomenon known as ocean acidification (OA). A primary class of marine organism behavior is impacted by OA, in particular, olfactory-mediated behaviors related to foraging and feeding success. However, the biological mechanisms responsible for the documented impairment of foraging and feeding-related behaviors remains in question. We find that juvenile American lobsters (H. americanus) exhibit significantly faster rates of total feeding times in response to short-term low pH exposure. However, total search time and total handling time were not affected by pH when examined …

Do Duckweeds Adapt To Water And Microbes?, Ava M. Rose

Honors Theses and Capstones

Organisms in their home environment sometimes outperform transplanted individuals native to other areas, in a phenomenon termed local adaptation. While local adaptation is traditionally considered to be driven by the abiotic factors of an environment, scientists have recently increased consideration of biotic factors. Specifically, of interest to many is host-associated microbiomes, which can alter host trait expression. As the desire to commercialize microbiome treatments for agriculture and medicine grows, it is important to analyze the potential value of local microbiomes, which may be adapted to their hosts, or to which local hosts may have adapted. Using Lemna minor (duckweed) as …

Financial Considerations For U.S. Equine Rescues: A Comparison Of Adoption-Based Vs. Sanctuary-Based Organizations, Carolyn V. Frank

Honors Theses and Capstones

No abstract provided.

The Continuation Of Thoroughbred Racing: A Dual Mission, Isabella V. Williams

Honors Theses and Capstones

Thoroughbred racing is the highest-profile segment of the equine industry in the United States and around the world, accounting for 50% of industry jobs and 25% of industry revenue. Public interest in the sport is rising thanks to social media and celebrity involvement at marquee events like the Kentucky Derby, leading to increased scrutiny of the sport, posing a high risk to human and equine participants alike. Public outcry at equine injuries in particular threatens the sport’s social license to operate (SLO), making it critical to protect the “whole-life welfare” of the animals involved in the sport to ensure its …

Drosophila Parasitoids Go To Space: Unexpected Effects Of Spaceflight On Hosts And Their Parasitoids, Jennifer Chou, Johnny R. Ramroop, Amanda M. Saravia-Butler, Brian Wey, Matthew P. Lera, Medaya L. Torres, Mary Ellen Heavner, Janani Iyer, Siddhita D. Mhatre, Sharmila Bhattacharya, Shubha Govind

Publications and Research

While fruit flies (Drosophila melanogaster) and humans exhibit immune system dysfunction in space, studies examining their immune systems’ interactions with natural parasites in space are lacking. Drosophila parasitoid wasps modify blood cell function to suppress host immunity. In this study, naive and parasitized ground and space flies from a tumor-free control and a blood tumor-bearing mutant strain were examined. Inflammation-related genes were activated in space in both fly strains. Whereas control flies did not develop tumors, tumor burden increased in the space-returned tumor-bearing mutants. Surprisingly, control flies were more sensitive to spaceflight than mutant flies; many of their …

South America Holds The Greatest Diversity Of Native Daisies (Asteraceae) In The World: An Updated Catalogue Supporting Continental-Scale Conservation, Andrés Moreira-Muñoz, Marcelo Monge, Mariana A. Grossi, Fabio Andrés Ávila, Vanezza Morales-Fierro, Gustavo Heiden, Berni Britto, Stephan Beck, Jimi N. Nakajima, Vanina G. Salgado, Juan Facundo Rodríguez-Cravero, Diego G. Gutiérrez

Publications and Research

Asteraceae is the world’s richest plant family and is found on all continents, in environments ranging from the coast to the highest mountains. The family shows all growth forms and, as in other angiosperm families, species richness is concentrated in tropical regions. South America has the highest diversity of Asteraceae in the world, yet taxonomic and distributional knowledge gaps remain. This study compiles an updated catalog of Asteraceae native to South America, based on national and regional checklists and ongoing large-scale flora projects. The resulting checklist includes a total of 6,940 species and 564 genera native to South America to …

Comprehensive Analysis Of Craniopharyngioma: Epidemiology, Clinical Characteristics, Management Strategies, And Role Of Radiotherapy, Amir Mushtaq, Mohsin Fayaz, Abdul Rashid Bhat, Abbas F Abdul Hussein, Gianluca Ferini, Giuseppe Emmanuele Umana, Gianluca Scalia, Feroze Ahmad Mir, Aizul Khursheed, Bipin Chaurasia

Faculty, Staff and Student Publications

BACKGROUND/AIM: Craniopharyngiomas pose challenges in diagnosis and management due to their rare occurrence and diverse clinical manifestations. This study aimed to provide a comprehensive analysis of cranio-pharyngioma, including its epidemiological trends, clinical presentations, radiological characteristics, surgical interventions, and the role of radiotherapy.

PATIENTS AND METHODS: A retrospective observational study was conducted on 23 patients diagnosed with craniopharyngioma at our hospital from August 2017 to July 2019. Data regarding demographics, clinical presentation, radiological findings, surgical interventions, and adjuvant therapies were collected and analyzed.

RESULTS: Craniopharyngiomas exhibited a bimodal age distribution, with peaks in childhood and late adulthood. Clinical presentations varied between …

A Relay Velocity Model Infers Cell-Dependent Rna Velocity, Shengyu Li, Pengzhi Zhang, Weiqing Chen, Lingqun Ye, Kristopher W Brannan, Nhat-Tu Le, Jun-Ichi Abe, John P Cooke, Guangyu Wang

Faculty, Staff and Student Publications

RNA velocity provides an approach for inferring cellular state transitions from single-cell RNA sequencing (scRNA-seq) data. Conventional RNA velocity models infer universal kinetics from all cells in an scRNA-seq experiment, resulting in unpredictable performance in experiments with multi-stage and/or multi-lineage transition of cell states where the assumption of the same kinetic rates for all cells no longer holds. Here we present cellDancer, a scalable deep neural network that locally infers velocity for each cell from its neighbors and then relays a series of local velocities to provide single-cell resolution inference of velocity kinetics. In the simulation benchmark, cellDancer shows robust …