Life Sciences Commons^™

Interactions Between New Zealand Mud Snails (Potamopyrgus Antipodarum) And Toxic Cyanoabacteria (Anabaena) In Baduwa’T (Mad River), Humboldt County, California, Cecilia F. Kennelly

Cal Poly Humboldt theses and projects

Invasive species in freshwater systems are circulated around the globe, wreaking havoc on natural communities. The New Zealand mud snail (Potamopyrgus antipodarum) is a very successful aquatic invader that has established populations in freshwater streams, lakes, and rivers around the globe. The goal of this project was to evaluate the interactions of invasive New Zealand mud snails (NZMS) and toxic cyanobacteria Anabaena in the Mad River in Humboldt County, California. These two species of concern are found in similar habitats along the river, but their whether NZMS consume Anabaena or affect toxin production is unknown. First, I conducted …

Barn Owls Exert Top-Down Effects On The Abundance And Behavior Of Rodent Pests, Katherine C. Larson

Cal Poly Humboldt theses and projects

Predators can exert top-down effects on their prey by direct consumption, by imposing risk of predation, or a combination of both. However, empirical evidence for these effects is mixed and inconsistent. Barn owls in agriculture present a system with attributes that should, according to theory, lead to strong top-down effects, but research is limited and the distinction between effects on prey abundance and behavior remains unresolved. Understanding the top-down effect of predators on pests is a cornerstone of integrated pest management and important where barn owls may contribute to the control of rodents on farms. Vegetation can mediate the top-down …

Persistent Net Release Of Carbon Dioxide And Methane From An Alaskan Lowland Boreal Peatland Complex, Eugénie S. Euskirchen, Colin W. Edgar, Evan Kane, Mark P. Waldrop, Rebecca B. Neumann, Kristen L. Manies, Thomas A. Douglas, Catherine Dieleman, Miriam C. Jones, Merritt R. Turetsky

Michigan Tech Publications

Permafrost degradation in peatlands is altering vegetation and soil properties and impacting net carbon storage. We studied four adjacent sites in Alaska with varied permafrost regimes, including a black spruce forest on a peat plateau with permafrost, two collapse scar bogs of different ages formed following thermokarst, and a rich fen without permafrost. Measurements included year-round eddy covariance estimates of net carbon dioxide (CO2), mid-April to October methane (CH4) emissions, and environmental variables. From 2011 to 2022, annual rainfall was above the historical average, snow water equivalent increased, and snow-season duration shortened due to later snow return. Seasonally thawed active …

Valorization Of Spent Vetiver Roots For Biochar Generation, Sameer Neve, Dibyendu Sarkar, Manas Warke, Teresa Bandosz, Rupali Datta

Michigan Tech Publications

Vetiver root is widely used to produce essential oils in the aromatherapy industry. After the extraction of oil, the roots are disposed of as waste. The central objective of this research was to explore the conversion of this waste into a resource using a circular economy framework. To generate biochar, vetiver roots were pyrolyzed at different temperatures (300, 500, and 700 °C) and residence times (30, 60, and 120 min). Analysis showed the root biochar generated at 500 °C and held for 60 min had the highest surface area of 308.15 m2/g and a yield of 53.76%, in addition to …

Season Influences Interspecific Responses Of Canopy-Forming Kelps To Future Warming And Acidification At High Latitude, Lauren E. Bell, Lily Westphal, Evan O' Brien, Jason A. Toy, Haleigh Damron, Kristy J. Kroeker

Biological Sciences Faculty Publications

Variability in primary producers' responses to environmental change may buffer higher trophic levels against shifts in basal resource composition. Then again, in instances where there is a lack of functional redundancy because consumers rely on a few species to meet their energetic requirements at specific times of the year, altered community production dynamics may significantly impact food web resilience. In high-latitude kelp forests, a complementary annual phenology of seaweed production supports coastal marine consumers' metabolic needs across large seasonal variations in their environment. Yet, marine consumers in these systems may face significant metabolic stress under the pronounced low pH conditions …

Listeria Adhesion Protein Orchestrates Caveolae-Mediated Apical Junctional Remodeling Of Epithelial Barrier For Listeria Monocytogenes Translocation, Rishi Drolia, Donald B. Bryant, Shivendra Tenguria, Zuri A. Jules-Culver, Jessie Thind, Breanna Amelunke, Donqi Liu, Nicholas L. F. Gallina, Krishna K. Mishra, Manalee Samaddar, Manoj R. Sawale, Dharmendra K. Mishra, Abigail D. Cox, Arun K. Bhunia

Biological Sciences Faculty Publications

The cellular junctional architecture remodeling by Listeria adhesion protein-heat shock protein 60 (LAP-Hsp60) interaction for Listeria monocytogenes (Lm) passage through the epithelial barrier is incompletely understood. Here, using the gerbil model, permissive to internalin (Inl) A/B-mediated pathways like in humans, we demonstrate that Lm crosses the intestinal villi at 48 h post-infection. In contrast, the single isogenic (lap− or ΔinlA) or double (lap−ΔinlA) mutant strains show significant defects. LAP promotes Lm translocation via endocytosis of cell-cell junctional complex in enterocytes that do not display luminal E-cadherin. In comparison, InlA facilitates …

Targeting Ocean Conservation Outcomes Through Threat Reduction, Joseph A. Turner, Malcolm Starkey, Nicholas K. Dulvy, Frank Hawkins, Louise Mair, Adeline Serckx, Thomas Brooks, Beth Polidoro, Stuart H. M. Butchart, Kent Carpenter, Minna Epps, Rima W. Jabado, Nicholas B. W. Macfarlane, Leon Bennun

Biological Sciences Faculty Publications

Nations have committed to reductions in the global rate of species extinctions through the Sustainable Development Goals 14 and 15, for ocean and terrestrial species, respectively. Biodiversity loss is worsening despite rapid growth in the number and extent of protected areas, both at sea and on land. Resolving this requires targeting the locations and actions that will deliver positive conservation outcomes for biodiversity. The Species Threat Abatement and Restoration (STAR) metric, developed by a consortium of experts, quantifies the contributions that abating threats and restoring habitats in specific places offer towards reducing extinction risk based on the IUCN Red List …

Assessing The Value And Knowledge Gains From An Online Tick Identification And Tick-Borne Disease Management Course For The Southeastern United States, Catherine A. Lippi, Holly D. Gaff, Alexis L. White, Sadie J. Ryan

Biological Sciences Faculty Publications

Background

Tick-borne diseases are a growing public health threat in the United States. Despite the prevalence and rising burden of tick-borne diseases, there are major gaps in baseline knowledge and surveillance efforts for tick vectors, even among vector control districts and public health agencies. To address this issue, an online tick training course (OTTC) was developed through the Southeastern Center of Excellence in Vector-Borne Diseases (SECOEVBD) to provide a comprehensive knowledge base on ticks, tick-borne diseases, and their management.

Methods

The OTTC consisted of training modules covering topics including tick biology, tick identification, tick-borne diseases, and public health, personal tick …

Deconstructing The Mangrove Carbon Cycle: Gains, Transformation, And Losses, M. F. Adame, N. Cormier, P. Taillardat, N. Iram, A. Rovai, T. M. Sloey, E. S. Yando, J. F. Blanco-Libreros, M. Arnaud, T. Jennerjahn, C. E. Lovelock, D. Friess, G. M. S. Reithmaier, C. A. Buelow, S. M. Muhammad-Nor, R. R. Twilley, R. A. Ribeiro

Biological Sciences Faculty Publications

Mangroves are one of the most carbon-dense forests on the Earth and have been highlighted as key ecosystems for climate change mitigation and adaptation. Hundreds of studies have investigated how mangroves fix, transform, store, and export carbon. Here, we review and synthesize the previously known and emerging carbon pathways in mangroves, including gains (woody biomass accumulation, deadwood accumulation, soil carbon sequestration, root and litterfall production), transformations (food web transfer through herbivory, decomposition), and losses (respiration as CO₂ and CH₄, litterfall export, particulate and dissolved carbon export). We then review the technologies available to measure carbon fluxes in …

Wild Sun Bears (Helarctos Malayanus) Exhibit Aseasonality In Parturition, Zachary A. David, Brian Crudge, Matt Hunt, Kirsty Officer, Vuthy Choun, Barbara Durrant, Megan A. Owen, Morokot Long, John P. Whiteman

Biological Sciences Faculty Publications

Seasonal reproduction can provide species with fitness advantages by allowing the birth of young to coincide with favorable environmental conditions, particularly in environments with high seasonal and intra-annual variation in these conditions. Seven of the eight species in the bear family (Ursidae) reproduce seasonally in both managed care and the wild; however, data for the eighth species, the sun bear (Helarctos malayanus), is unclear. Sun bears have been observed to reproduce throughout the year in managed care, yet currently there are no clear data of birth timing for wild sun bear cubs. Here we investigate the seasonality of …

Active Facilitation Of Helper Dispersal By Parents And Siblings In The Cooperatively Breeding Acorn Woodpecker, Natasha D. G. Hagemeyer, Walter D. Koenig, Eric L. Walters

Biological Sciences Faculty Publications

Offspring that delay dispersal in cooperatively breeding species have been hypothesized to gain direct fitness benefits via parental facilitation—being passively tolerated on their natal territory by their parents—thereby enjoying enhanced survival and increased probability of acquiring a breeding position in the population. Here we describe active facilitation in the acorn woodpecker (Melanerpes formicivorus) by parents and siblings assisting the dispersal of helpers in their social group. Helpers in this species compete for reproductive opportunities in “power struggles” that take place when all breeders of one sex die or disappear, creating a reproductive vacancy. Individuals compete at power struggles …

A Taxonomic Revision Of The Genus Hydnora (Hydnoraceae), Sebastian A. Hatt, Chris J. Thorogood, J. F. Bolin, Lytton J. Musselman, Duncan D. Cameron, Olwen M. Grace

Biological Sciences Faculty Publications

A systematic monograph is presented for Hydnora (Hydnoraceae), a poorly known genus of holoparasitic plants distributed across Africa, Madagascar and southern Arabia. Species of Hydnora are characterised by their underground habit, unusual fleshy flowers and complete absence of leaves or photosynthetic tissue. This is the first detailed monograph of the genus Hydnora since 1935 and is informed by a comprehensive survey of herbarium specimens and literature. Detailed descriptions, full synonymy, distribution maps and discussion concerning confusable taxa are provided for each species, along with notes on ethnobotany, ecology and conservation. We place particular emphasis on the taxonomic value of osmophore …

Salmonella Detection In Food Using A Hek-Htlr5 Reported Cell-Based Sensor, Esma Eser, Victoria A. Felton, Rishi Drolia, Arun K. Bhunia

Biological Sciences Faculty Publications

The development of a rapid, sensitive, specific method for detecting foodborne pathogens is paramount for supplying safe food to enhance public health safety. Despite the significant improvement in pathogen detection methods, key issues are still associated with rapid methods, such as distinguishing living cells from dead, the pathogenic potential or health risk of the analyte at the time of consumption, the detection limit, and the sample-to-result. Mammalian cell-based assays analyze pathogens’ interaction with host cells and are responsive only to live pathogens or active toxins. In this study, a human embryonic kidney (HEK293) cell line expressing Toll-Like Receptor 5 (TLR-5) …

Baby's First Jets: A Kinematic And Hydrodynamic Analysis Of Turning In Cuttlefish Hatchlings, Alissa M. Ganley, Paul S. Krueger, Ian K. Bartol

Biological Sciences Faculty Publications

Turning is an important aspect of life underwater, playing integral roles in predator avoidance, prey capture, and communication. While turning abilities have been explored in a diversity of adult nekton, little is currently known about turning in early ontogeny, especially for cephalopods. In this study, we investigated the turning abilities of hatchling common cuttlefish (Sepia officinalis, n = 49) and dwarf cuttlefish (Sepia bandensis, n = 30), using both kinematic and wake-based analyses. Using body tracking software and particle image velocimetry (PIV), we found that S. officinalis turned faster than S. bandensis, but both species …

Ai And Ml-Based Risk Assessment Of Chemicals: Predicting Carcinogenic Risk From Chemical-Induced Genomic Instability, Ajay Vikram Singh, Preeti Bhardwaj, Peter Laux, Prachi Pradeep, Madleen Busse, Andreas Luch, Akihiko Hirose, Christopher J. Osgood, Michael W. Stacey

Biological Sciences Faculty Publications

Chemical risk assessment plays a pivotal role in safeguarding public health and environmental safety by evaluating the potential hazards and risks associated with chemical exposures. In recent years, the convergence of artificial intelligence (AI), machine learning (ML), and omics technologies has revolutionized the field of chemical risk assessment, offering new insights into toxicity mechanisms, predictive modeling, and risk management strategies. This perspective review explores the synergistic potential of AI/ML and omics in deciphering clastogen-induced genomic instability for carcinogenic risk prediction. We provide an overview of key findings, challenges, and opportunities in integrating AI/ML and omics technologies for chemical risk assessment, …

Natural Enemies Of Varroa Destructor Identified From Eastern North America Honey Bee Colonies: A Biological Survey Of Candidates For Mite Control From Maryland, Usa, Francisco Posada-Flórez, Daniel Sonenshine, Jay Evans, Dawn Boncristiani, Monica Pava-Ripoll, Steven Cook

Biological Sciences Faculty Publications

A survey was conducted of microbes and arthropods associated with Varroa destructor (Acari: Varroidae) mites (hereafter, Varroa) collected from honey bee colonies in Maryland, USA. Live Varroa were challenged with samples of both microbes and arthropods and assessed for their potential as biocontrol agents against the mites. To our knowledge, this is the first survey of natural enemies of Varroa conducted in North American honey bee colonies. The survey revealed 21 fungi, 25 bacteria, one nematode, and 8 arthropod species associated with Varroa in honey bee colonies, and from challenge assays using 11 of these, including 4 species of …

Abalones At Risk: A Global Red List Assessment Of Haliotis In A Changing Climate, Howard Peters, Gina M. Ralph, Laura Rogers-Bennett

Biological Sciences Faculty Publications

There is increasing awareness that marine invertebrates such as abalones are at risk from the combined stressors of fishing and climate change. Abalones are an important marine fishery resource and of cultural importance to Indigenous and non-Indigenous people. A highly priced marine delicacy, they are inherently vulnerable: individuals are slow-growing and long-lived and successful reproduction requires dense assemblages. However, their global conservation status is poorly understood. Using IUCN Red List methodology, we assessed the extinction risk to all 54 species of abalone (genus Haliotis). Of the 21 fished commercially for human consumption either now and/or in the past, 15 …

State Of Coastal And Marine Resources: Sablayan, Occidental Mindoro, Ma Celina J. Añonuevo, Jemelyn Grace P. Baldisimo, Denmark B. Racamara, Mary Joyce P. Velos, Joseph Benedict T. Garcia, Frederico B. Sabban, Joey P. Cabasan, Rhea Mae Luciano, Darryl Anthony Valiño, Lovely Joy Heyres, Hazel O. Arceo

Biological Sciences Faculty Publications

[Introduction] Sablayan, situated on the western side of Mindoro Island facing the WPS (12.8564° N, 120.9101° E), is a first-class municipality in the province of Occidental Mindoro with a total land area of 2,188.8km² that is divided into 22 barangays, nine of which are along the coast. The coastline stretches for 115 km, with municipal waters covering approximately 750,000 km² (LGU-Sablayan 2008).

The Apo Reef Natural Park, one of its most prominent features, is located approximately 33 km off the coast (Figure 2.9-1) The Apo Reef Natural Park is a protected coral reef system under presidential proclamation 868 (1996) encompassing …

State Of Coastal And Marine Resources: Lian, Batangas, Joseph Benedict T. Garcia, Mary Joyce P. Velos, Jemelyn Grace P. Baldisimo, Joey P. Cabasan, Ma Celina J. Añonuevo, Denmark B. Recamara, Hazel O. Arceo

Biological Sciences Faculty Publications

[Introduction] Lian (13.9852° N, 120.6545° E) is a third-class municipality in the northwestern part of the province of Batangas. It is bounded on the north by the municipality of Nasugbu, on the east by the municipalities of Tuy and Balayan, on the west by the WPS, and on the south by the municipality of Catatagan (Figure 2.7-1). It has a total land area of 76.80km² that is divided into 19 barangays, six of which are coastal and two are integrated coastal barangays (LGU-Lian 2013). Its total population is 56,280 individuals in 15,991 households as of 2020 (PSA 2020).

The main …

Taxonomic Status, Distribution And Conservation Of The Critically Endangered Plant Psychotria Angustata (Rubiaceae), Walter Simbaña, Alan Tye, Timothy J. Motley, Uno Eliasson

Biological Sciences Faculty Publications

Simbaña, W.; A. Tye, T. J. Motley & U. Eliasson. 2024. Taxonomic status, distribution and conservation of the Critically Endangered plant Psychotria angustata (Rubiaceae), endemic to Floreana Island, Galapagos. Darwiniana, nueva serie 12(2): 204-219.

Two species of Psychotria have been recognised in the Galapagos Islands, both understorey shrubs endemic to the archipelago: P. rufipes Hook.f. occurs on seven islands, including Floreana, while P. angustata Andersson is endemic to Floreana. Since their mid-19th century discovery, the taxonomic and ecological status of these species has been uncertain. To clarify this, we examined historical specimens and numerous recent collections of both species, and …

Selection ​For Rain Gardens And Other Stormwater Installations​, Catherine Neal, Amy Papineau

UNH Cooperative Extension

No abstract provided.

Pseudomonas Mosselii Improves Cold Tolerance Of Asian Rice (Oryza Sativa L.) In A Genotype-Dependent Manner By Increasing Proline In Japonica And Reduced Glutathione In Indica Varieties, Nasim Maghboli Balasjin, James Maki, Michael Schläppi

Biological Sciences Faculty Research and Publications

Cold stress is an important factor limiting rice production and distribution. Identifying factors that contribute to cold tolerance in rice is of primary importance. While some plant specific genetic factors involved in cold tolerance have been identified, the role of the rice microbiome remains unexplored. In this study, we evaluated the influence of plant growth promoting bacteria (PGPB) with the ability of phosphate solubilization on rice cold tolerance and survival. To reach this goal, inoculated and uninoculated 2-week-old seedlings were cold stressed and evaluated for survival and other phenotypes such as electrolyte leakage (EL) and necessary elements for cold tolerance. …

Tarantula Toxin Transcripts And Toxin Structure Prediction By Alphafold, Christine Vega, Rebekkah Sepulveda, Ying Jia

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Tarantula venom predominantly comprises peptides and small proteins (venom toxins). To explore this further, we generated a combined gland cDNA library from five different tarantula species. Subsequently we randomly sequenced 752 clones, which led to the identification of sixteen distinct toxin groups, including one novel group. Each group consists of a minimum of three identical sequences. A comprehensive analysis of these toxin groups was carried out, and we simulated the 3D structures of certain predicted toxins. These transcripts can be used to the production of venom toxins through recombinant techniques, offering promising applications in various biomedical applications.

Effects Of Unilateral Lower Limb Suspension On Quadriceps’ Structure And Contractile Function, Eve N. Elmore

Theses and Dissertations--Kinesiology and Health Promotion

BACKGROUND: Muscle unloading such as bed rest or space flight decreases strength and cross-sectional area concomitant with a slow-to-fast fiber type shift, of anti-gravitational muscles such as the quadriceps. Diffusion tensor imaging (DTI) quantifies tissue anisotropic properties such as Fractional Anisotropy, Mean Diffusivity, Axial Diffusivity, and Radial Diffusivity, as well as muscle volume, fascicle length, and physiological cross-sectional area, and has recently been adapted to assess skeletal muscle structure. In skeletal muscle, anisotropic properties correlate with muscle fiber type and contractile function and may be responsive to unloading-induced atrophy.

PURPOSE: To assess the changes in quadriceps’ size, anisotropy, and contractile …

Influence Of Deception Of Task Duration On The Sex-Specific Physiological, Neuromuscular, And Affective Responses To Rpe-Clamp Cycle Ergometry, Pasquale Succi

Theses and Dissertations--Kinesiology and Health Promotion

Several theories exist to explain the interaction of sensory afferent feedback and efferent feedforward signals on the generation of a rating of perceived exertion (RPE) which influence endurance exercise pacing and performance. Within these theories, exists the premise that the expected duration of exercise plays a role within the generation of an individual’s RPE and their endurance performance. Previous work has demonstrated equivocal findings of this influence, while other research has suggested potential differences between males and females. However, an RPE-clamp model, where the external workload is modulated to maintain a constant perceptual intensity, has not previously been used to …

Altered Gait Mechanics In Marfan Syndrome, Amara G. Sharp

Theses and Dissertations--Kinesiology and Health Promotion

FBN1 is a gene responsible for the production of fibrillin, a major component of connective tissue throughout the body including skeletal muscle tissue. In individuals with Marfan Syndrome these mutations in FBN1 result in widespread muscle weakness and a correspondingly higher incidence of lower extremity joint pain. Despite the widespread musculoskeletal impacts of Marfan Syndrome there is a dearth of research on the potential impacts of Marfan Syndrome on gait mechanics that may be related to the high incidence of lower extremity joint pain in the Marfan Syndrome population. Therefore, the purpose of this study was to determine the potential …

Serum Interleukin-22 In Non-Segmental Vitiligo, Fatma Faisal El Dakrory, Maha Elsayed Mahmoud Elsayed, Yousra Ibrahim El-Tantawy Sadeq, Shereen Ezzalregal Alashry

Mansoura Medical Journal

Background: A depigmenting skin condition called vitiligo affects 0.5–2 percent of people globally. It seems to be caused by an interaction between neurological, immunological, and genetic factors. Vitiligo is mediated by cell-mediated reactions, as Th1/Th17 and Tc1 cells. It is thought that the Th17 cell's most specialized cytokine isIL-22. Aim: To explore the blood levels of IL-22 in non-segmental vitiligo and to investigate whether it can be a biomarker in monitoring disease activity and it’s correlation with disease severity. Patient and methods: This study included thirty healthy individuals and sixty non-segmental vitiligo cases (30 with stable vitiligo and 30 with …

Ultrasound Guided Quadratus Lumborum Block Versus Transversus Abdominis Plane Block As Postoperative Analgesia In Patients Undergoing Abdominal Cancer Surgery, Ghada Fouad Amer, Nevert Adel Abdel Ghaffar

Mansoura Medical Journal

Background: Abdominal surgeries are often linked with post-operative pain (POP). The transverse abdominal plane (TAP) block is an efficient method for POP management in these patients. The quadratus lumborum (QL) block (QLB) is another method for the POP control. The current study aims to assess the efficiency and safety of QLB and TAP block for POP control after abdominal cancer surgeries. Methods: 98 cases scheduled for abdominal cancer surgery by using general anesthesia (GA) were comprised in this randomized study. They were randomly divided into 2 equal groups :(QL group) patient received GA plus bilateral QLB, (TAP group) patient received …

Assessment Of Response Of Enteropathic Spondyloarthritis To Different Types Of Biological Therapy; An Egyptian Single Center Study, Fatma Abozeid, Marwa Besar

Mansoura Medical Journal

Introduction: Arthropathy is one of the most prevalent extraintestinal symptoms of inflammatory bowel disease (IBD). It may be either peripheral or axial spondyloarthropathy. Biological treatment as anti-tumor necrosis factor alpha (TNF𝛼) or anti-interleukin 12/23 (anti IL 12/23) have shown efficacy in controlling gut inflammation and reducing joint manifestation. Our aim of the study was to assess the different aspects of response (laboratory , endoscopically and radiologically) of axial arthropathy associated with IBD patients after receiving biological drugs including: anti TNF (infliximab and adalimumab)& anti IL 12/23 (ustekinumab)

Patients & methods:A single center observational study was conducted through one …

Coload Effect On Cardiac Output Measurement Using Transthoracic Echocardiography In Preeclamptic Patients Undergoing Cesarean Delivery, Sherine Ali Elsayed Bakrey, Aya Hasan Masoud Abdelhady, Alaa El-Din Mazy Abdou Mazy, Maha Ahmed Abo-Zeid Salim

Mansoura Medical Journal

Background: During cesarean delivery (CD) for preeclamptic patients under spinal anaesthesia (SA), much intravenous fluids are avoided. Through transthoracic echocardiography, study conducted to assess the hypothesis that colloid plus crystalloid coload will maintain the cardiac output (CO) than crystalloid fluid alone. Methods: Thirty-four preeclamptic patients, scheduled for elective CD, under SA, were allocated according to the fluid coloading into two groups; Crystalloid group (group I); in which the patients received Ringer’s acetate or Crystalloid-colloid group (group II); in which the patients received 6% Hydroxyethyl starch and Ringer’s acetate. The primary outcome was CO while, left ventricular end diastolic diameter (LVEDD), …