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2023

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Articles 241 - 270 of 13839

Full-Text Articles in Life Sciences

Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm Dec 2023

Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm

Turkish Journal of Veterinary & Animal Sciences

Genetic diseases play an increasingly important role in cattle breeding as breeding technologies such as artificial insemination has an inborn risk of disseminating deleterious genes, especially from high merit breeding sires. Cholesterol deficiency (CD) is a metabolic disorder first recognized in Holstein cattle in 2015. The condition is characterized by chronic diarrhea and unthriftiness in calves being homozygous for the defective allele. The prevalence of carriers of CD in Turkish cattle is at present unknown and therefore 700 heifers and cows of different breeds, including 300 Holsteins were genotyped. Of the tested Holsteins, 2.0% turned out to be carriers of …


Functional Training Promotes Benefits In The Physical Condition Of Horses, Paula Gomes Rodrigues, Camila Fernanda Fidêncio, Katia De Oliveira, Camilla Mendonça Silva, Clistenes Gomes Oliveira, José Miradelson Oliveira Carvalho, Anselmo Domingos Ferreira Santos, Gregorio Murilo De Oliveira Junior, José Camisão De Souza Dec 2023

Functional Training Promotes Benefits In The Physical Condition Of Horses, Paula Gomes Rodrigues, Camila Fernanda Fidêncio, Katia De Oliveira, Camilla Mendonça Silva, Clistenes Gomes Oliveira, José Miradelson Oliveira Carvalho, Anselmo Domingos Ferreira Santos, Gregorio Murilo De Oliveira Junior, José Camisão De Souza

Turkish Journal of Veterinary & Animal Sciences

Horses that perform constant physical activity related to sport or work are subject to musculoskeletal disorders. Thus, the objective was to evaluate the effectiveness of a functional training program to promote physical conditions (epaxial musculature biometry and linear kinematics) and behavior traits in patrol horses. Sixteen crossbred horses were randomly distributed in two experimental treatments: not submitted (control group) and submitted (test group) to a functional exercise program. The training program exercises were performed three times per week for 90 days and consisted of dynamic mobilization techniques, reflexive mobilization, dynamic imbalance, and passive stretching. The studied variables were Longissimus dorsi …


Tuftelin1 Drives Experimental Pulmonary Fibrosis Progression By Facilitating Stress Fiber Assembly, Caoyuan Niu, Kai Xu, Yanan Hu, Yanling Jia, Yuexia Yang, Xiaoyue Pan, Ruyan Wan, Hui Lian, Qiwen Wang, Juntang Yang, Yajun Li, Ivan Rosas, Lan Wang, Guoying Yu Dec 2023

Tuftelin1 Drives Experimental Pulmonary Fibrosis Progression By Facilitating Stress Fiber Assembly, Caoyuan Niu, Kai Xu, Yanan Hu, Yanling Jia, Yuexia Yang, Xiaoyue Pan, Ruyan Wan, Hui Lian, Qiwen Wang, Juntang Yang, Yajun Li, Ivan Rosas, Lan Wang, Guoying Yu

Children’s Nutrition Research Center Staff Publications

Background: Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease (ILD) with unknown etiology, characterized by sustained damage repair of epithelial cells and abnormal activation of fibroblasts, the underlying mechanism of the disease remains elusive.

Methods: To evaluate the role of Tuftelin1 (TUFT1) in IPF and elucidate its molecular mechanism. We investigated the level of TUFT1 in the IPF and bleomycin-induced mouse models and explored the influence of TUFT1 deficiency on pulmonary fibrosis. Additionally, we explored the effect of TUFT1 on the cytoskeleton and illustrated the relationship between stress fiber and pulmonary fibrosis.

Results: Our results demonstrated a significant …


Regulation Of Mrna And Circrna As A Cause And Consequence Of Aging In Caenorhabditis Elegans, Rebekah Napier-Jameson Dec 2023

Regulation Of Mrna And Circrna As A Cause And Consequence Of Aging In Caenorhabditis Elegans, Rebekah Napier-Jameson

Biological Sciences Theses and Dissertations

A role for RNA binding proteins in regulating lifespan has emerged. We identified a pair of neuronal RBPs, exc-7 and mbl-1, which display synthetic lifespan defects. Such a strong synthetic phenotype represented an opportunity to use transcriptomics to search for potential causative targets that are synthetically regulated. We identified a small handful of genes synthetically dysregulated in double mutants and systematically tested each candidate gene for functional contribution to the exc-7; mbl-1 lifespan phenotype. We identified one such gene, the ion transporter nhx-6, which is highly upregulated in double mutants. Overexpression of nhx-6 causes reduced lifespan, and deletion …


Deep Learning For Microbiome-Based Integrative Modeling And Microbial Biomarkers Identification, Sen Yang Dec 2023

Deep Learning For Microbiome-Based Integrative Modeling And Microbial Biomarkers Identification, Sen Yang

Statistical Science Theses and Dissertations

The human microbiome, comprising trillions of microorganisms, plays a pivotal role in modulating host physiology via molecular and metabolite exchanges. One of the major challenges in this field lies in the effective integration of microbiome and metabolomics data, an achievement that holds the promise of substantially enhancing the precision of disease prediction. However, many datasets prioritize microbiome data while neglecting paired metabolome information. Additionally, the prevalent analytical tools face challenges in effectively merging these intricate datasets, leading to possible misinterpretations and reduced prediction accuracies.

To address these challenges, the first part of this research introduces the Microbiome-based Supervised Contrastive Learning …


Isolation And Characterization Of Bacteriophages From Fish Tank Samples, Oluwaseyi Oluwagbeminiyi Oladele-Ajose Dec 2023

Isolation And Characterization Of Bacteriophages From Fish Tank Samples, Oluwaseyi Oluwagbeminiyi Oladele-Ajose

Electronic Theses and Dissertations

Bacteriophages (phages) are viral obligate intracellular parasites that infect bacteria. Research has been carried out on alternative treatment plans for bacterial infections due to the increase in antibiotic resistance. Phages have been proposed as an alternative to antibiotics. This research focused on the isolation and characterization of bacteria from small aquariums. A total of 42 bacterial isolates were isolated from seven different fish tank water samples and were tested to determine if they possessed phages. Spot plaque assay revealed presence of lytic phage for only one of the isolated bacterial strains, which was determined to belong to Enterobacteriaceae family based …


The Effectiveness Of Garlic Extract Against Spodoptera Litura On Chili, Efrin Firmansyah, Cecep Hidayat, Anna Aina Roosda, Adinda Nurul Fatah Adi Pradana, R Soelistijono Dec 2023

The Effectiveness Of Garlic Extract Against Spodoptera Litura On Chili, Efrin Firmansyah, Cecep Hidayat, Anna Aina Roosda, Adinda Nurul Fatah Adi Pradana, R Soelistijono

Jurnal Kultivasi

Spodoptera litura is a polyphagous pest and attack many families of cultivated plants, such as chili plants. Application of synthetic pesticides is still the mainstay for controlling this pest in the field. To support eco-friendly plant protection management, it is important to explore potential natural materials such as garlic extract. This research aims to determine the potential of garlic extract in controlling S. litura on chili plants, and to see its indirect effect on chili yield. The research was carried out in two sites, in the laboratory and the field. The experiment used a completely randomized design with 6 treatments …


Callus Induction And Proliferation Of Centella Asiatica L. Generated From Leaves And Petioles In The Presence Of Dicamba And Bap, Suci Rahayu, Darmawan Saptadi, Chotimatul Azmi, Kusumawaty Kusumanegara, Tri Handayani, Ika Roostika, Nurliani Bermawie, Haris Maulana Dec 2023

Callus Induction And Proliferation Of Centella Asiatica L. Generated From Leaves And Petioles In The Presence Of Dicamba And Bap, Suci Rahayu, Darmawan Saptadi, Chotimatul Azmi, Kusumawaty Kusumanegara, Tri Handayani, Ika Roostika, Nurliani Bermawie, Haris Maulana

Jurnal Kultivasi

Centella's need for industrial raw materials is high as a medicinal plant. These needs can be met through rapid multiplication using tissue culture techniques. In this study, induction and proliferation for a callus of centella cv. Castina 3 was conducted in the MS basal medium plus 4 mgL-1 Dicamba withand enriched with 7 concentrations of BAP (0, 0.1, 0.3, 0.5, 0.7, 0.9, and 1.1 mgL-1). Two kinds of explant were used, i.e., leaf and petiole. The results revealed that the addition of BAP in MS plus Dicamba medim stimulated better and produced a higher callus growth rate, both from leaf …


Correlation Analysis Between Agronomic Character And Yield Of Padjadjaran Maize Hybrids At Medium And High Population Densities, Yuyun Yuwariah, Qinthara Nail Haysa, Damara Bakti Muhammad, Dedi Ruswandi, Fiky Yulianto Wicaksono Dec 2023

Correlation Analysis Between Agronomic Character And Yield Of Padjadjaran Maize Hybrids At Medium And High Population Densities, Yuyun Yuwariah, Qinthara Nail Haysa, Damara Bakti Muhammad, Dedi Ruswandi, Fiky Yulianto Wicaksono

Jurnal Kultivasi

Maize is a prospective food commodity that developed in Indonesia. The use of hybrid corn gives better yield than open-pollinated varieties. Spacing management in hybrid maize production can reduce competition between plants, so that the plant canopy and roots can grow optimally. This research aimed to compare the differences in the phenotypic performance of the agronomic characters of Padjadjaran maize hybrid, determine the dominant agronomic characteristics, and determine the correlation between the growth and yield characteristics at medium- and high-population densities. The experiment was carried out from December 2021 until May 2022 at Sanggar Penelitian, Latihan dan Pengembangan Pertanian (SPLPP) …


Quantifying The Influences Of Abiotic And Biotic Factors On Post-Fire Vegetation In The Southwestern Us, Joseph Lafayette Crockett Dec 2023

Quantifying The Influences Of Abiotic And Biotic Factors On Post-Fire Vegetation In The Southwestern Us, Joseph Lafayette Crockett

Biology ETDs

The ongoing warming associated with climate change is increasing the flammability of southwestern US dry forests. As larger fractions of the landscape are impacted by high-severity fire, the potential exists for forests to convert to non-forest. Given that the forested area in the southwestern US is an important contributor of ecosystem services, quantifying limitations to vegetation establishment is critical to informing post-fire recovery efforts and understanding how ongoing climate change will limit seedling establishment in the future. To that end, this dissertation focuses on post-fire vegetation recovery of southwestern forests. First, I report on the influence of precipitation and topography …


Characterizing The Ecology Of Plains (Spilogale Interrupta) And Eastern (Spilogale Putorius) Spotted Skunks: A Systematic Literature Review And Population Assessment In Kansas, Jenell De La Peña Dec 2023

Characterizing The Ecology Of Plains (Spilogale Interrupta) And Eastern (Spilogale Putorius) Spotted Skunks: A Systematic Literature Review And Population Assessment In Kansas, Jenell De La Peña

Electronic Theses & Dissertations

The plains spotted skunk (Spilogale interrupta) has recently been recognized as a distinct species from the eastern spotted skunk (Spilogale putorius). While once abundant across the central and eastern United States, both species have experienced population declines, resulting in their review for listing under the Endangered Species Act (1973). In Kansas, the plains spotted skunk has suffered a particularly dramatic decline, the last detection in the state having occurred in 2020. Additionally, due to the recent taxonomic split, many past studies reporting on S. putorius ecology actually describe findings of two species, potentially complicating our understanding …


A Preliminary Study On Floating Flexible Otec Cold Water Pipe, Nai Kuang Liang, Hai Kuan Peng Dec 2023

A Preliminary Study On Floating Flexible Otec Cold Water Pipe, Nai Kuang Liang, Hai Kuan Peng

Journal of Marine Science and Technology–Taiwan

Ocean Thermal Energy Conversion (OTEC) requires a large amount of cold seawater. The traditional rigid pipe, semi-rigidly fixed onto the ship bottom is not easy to install and disassemble, so the Floating Flexible Cold Water Pipe (FFCWP) flexibly connected to the ship is proposed. A small FFCWP was designed, fabricated, and successfully installed and recovered in the sea. The flexible pipe adopts commercially available fire ventilation snake pipe. The pipe wall material strength must be improved in the future. Assuming that the drag coefficient Cd value is 1.5, a numerical calculation is employed to simulate the FFCWP attitude from the …


Machine Learning With Multi-Source Data To Predict And Explain Marine Pilot Occupational Accidents, Gokhan Camliyurt, Youngsoo Park, Daewon Kim, Won Sik Kang, Sangwon Park Dec 2023

Machine Learning With Multi-Source Data To Predict And Explain Marine Pilot Occupational Accidents, Gokhan Camliyurt, Youngsoo Park, Daewon Kim, Won Sik Kang, Sangwon Park

Journal of Marine Science and Technology–Taiwan

Marine pilot occupational accidents during transfer to/from ships are the primary concern of the International Marine Pilots’ Association (IMPA) and industry professionals. There are multiple transfer methods for marine pilots, with the most common being the pilot boat. To reach the mother ship bridge, the following stages must be safely completed: car transfer, walking on the pier, pier to pilot boat, pilot transfer by boat, cutter to pilot ladder, mother ship freeboard climbing, and ship deck to the bridge. Each stage has its own risk. Previous accident records and expert opinions are commonly used to conduct a risk analysis and …


Evaluation Of Operational Performance Of Wusongkou Cruise Port Through Network Data Envelopment Analysis, Qian-Feng Wang, Guo-Ya Gan, Xin-Liang Ye, Hsuan-Shih Lee Dec 2023

Evaluation Of Operational Performance Of Wusongkou Cruise Port Through Network Data Envelopment Analysis, Qian-Feng Wang, Guo-Ya Gan, Xin-Liang Ye, Hsuan-Shih Lee

Journal of Marine Science and Technology–Taiwan

As major hubs for cruise berthing and passenger transfers, cruise ports in China were developing rapidly in the years before the COVID-19 pandemic because of an ever-growing regional market. Wusongkou Cruise Port is the most important port for cruise ships in China, and this study evaluated the operational performance of this port during 2011–2020. To this end, two-stage network data envelopment analysis was conducted to evaluate the port’s operation performance; subsequently, the change trajectory of the port’s operational efficiency during 2011–2020 was determined, and the potential reasons for the identified changes are discussed. Finally, suggestions for improving the operational performance …


Corrigendum To "Ssr-Based Molecular Diagnosis For Taiwan Tea Cultivars And Its Application In Identifying Cultivar Composition Of The Processed Tea" Dec 2023

Corrigendum To "Ssr-Based Molecular Diagnosis For Taiwan Tea Cultivars And Its Application In Identifying Cultivar Composition Of The Processed Tea"

Journal of Food and Drug Analysis

No abstract provided.


Acknowledgment Of Reviewers Dec 2023

Acknowledgment Of Reviewers

Journal of Food and Drug Analysis

No abstract provided.


Application Of The Us Food And Drug Administration’S Sentinel Routine Querying Tools To The Taiwan Sentinel Data Model-Formatted National Health Insurance Research Database, Fang-Ju Lin, Ling-Ya Huang, Chi-Chuan Wang, Sengwee Toh Dec 2023

Application Of The Us Food And Drug Administration’S Sentinel Routine Querying Tools To The Taiwan Sentinel Data Model-Formatted National Health Insurance Research Database, Fang-Ju Lin, Ling-Ya Huang, Chi-Chuan Wang, Sengwee Toh

Journal of Food and Drug Analysis

The U.S. Food and Drug Administration’s Sentinel System is a leading distributed data network for drug safety surveillance in the world. The National Health Insurance Research Database (NHIRD) in Taiwan was converted into the Taiwan Sentinel Data Model (TSDM) based on the Sentinel Common Data Model (SCDM) version 6.0.2. The goal of this study was to investigate the feasibility of applying the same study designs, analytic choices, and analytic tools as used by the U.S. Sentinel System to examine the same drug-outcome associations in the TSDM-formatted NHIRD. Four known drug-outcome associations previously examined by the U.S. Sentinel System were selected …


Isolation Of Glp-1 Enhancing Indolizidine Alkaloids From Boehmeria Formosana, Ching-Yi Lu, Zheng-Ying Huang, Meng-Chin Chen, Hui-Kang Liu, Shoei-Sheng Lee, Chia-Chuan Chang Dec 2023

Isolation Of Glp-1 Enhancing Indolizidine Alkaloids From Boehmeria Formosana, Ching-Yi Lu, Zheng-Ying Huang, Meng-Chin Chen, Hui-Kang Liu, Shoei-Sheng Lee, Chia-Chuan Chang

Journal of Food and Drug Analysis

Boehmeria formosana, with its related species, demonstrates anti-glycemic effect, inhibition of HBV production, anti-cancer activities, etc. Some indolizidine alkaloids from the same genus are bioactive but sensitive to light. To overcome this problem and obtain more phenanthroindolizidine alkaloids, isolation was performed in darkness, yielding 10 new indolizidine alkaloids and 17 known compounds. Among them, seven enhanced glucagon-like receptor 1 (GLP-1) activity at 50 mM, especially 14 and 6 (3.5- and 2.3-fold than the negative control). This procedure yielded bioactive indolizidine alkaloids with novel structures.


An Integrated Strategy To Evaluate Active Substances Of Astragali Radix-Carthami Flos Combination On The Treatment Of Cerebral Ischemia Reperfusion Injury Based On Tqsm Polypharmacokinetics And Pharmacodynamics, Qiang Zeng, Chang Li, Shouchao Xu, Yu He Dec 2023

An Integrated Strategy To Evaluate Active Substances Of Astragali Radix-Carthami Flos Combination On The Treatment Of Cerebral Ischemia Reperfusion Injury Based On Tqsm Polypharmacokinetics And Pharmacodynamics, Qiang Zeng, Chang Li, Shouchao Xu, Yu He

Journal of Food and Drug Analysis

As a classic herb pair, Astragali Radix-Carthami Flos (AR-CF) has revealed good biological activity in the treatment of cerebral ischemia/reperfusion injury (CI/RI), which remained to be further clarified together with the underlying efficacy related compounds for material basis. In this study, the nine formulations were obtained by L9(34) orthogonal array design of four active fractions (saponin and flavonoid extracted from AR, safflower yellow and safflower red extracted from CF). The concentrations of eleven components and the levels of four biochemical indicators in rat plasma were continuously detected after intragastric administration of nine formulations, respectively. The collected …


Cryptocaryone Induces Apoptosis In Human Hepatocellular Carcinoma Cells By Inhibiting Aerobic Glycolysis Through Akt And C-Src Signaling Pathways, Chen-Lin Yu, Yu-Wei Lai, Jih-Jung Chen, Jie-Jen Lee, Tsung-Hsien Chou, Chen-Chen Huang, Shih-Chia Liu, Guang-Wei Chen, Chung‐Hsin Tsai, Shih-Wei Wang Dec 2023

Cryptocaryone Induces Apoptosis In Human Hepatocellular Carcinoma Cells By Inhibiting Aerobic Glycolysis Through Akt And C-Src Signaling Pathways, Chen-Lin Yu, Yu-Wei Lai, Jih-Jung Chen, Jie-Jen Lee, Tsung-Hsien Chou, Chen-Chen Huang, Shih-Chia Liu, Guang-Wei Chen, Chung‐Hsin Tsai, Shih-Wei Wang

Journal of Food and Drug Analysis

Hepatocellular carcinoma (HCC) is the most common form of liver cancer, with the second highest mortality rate in all cancer. Energy reprogramming is one of the hallmarks of cancer, and emerging evidence showed that targeting glycolysis is a promising strategy for HCC treatment. Cryptocaryone has been shown to display promising anti-cancer activity against numerous types of cancer. Previous study also indicated that cryptocaryone induces cytotoxicity by inhibiting glucose transport in cancer cells, but the detailed mechanism still needs to be elucidated. Therefore, this study aimed to investigate the relationship between the anti-cancer effect and glycolytic metabolism of cryptocaryone in human …


Dehulled Adlay (Coix Lachryma-Jobi L.) Ameliorates Hepatic Gluconeogenesis And Steatosis In Streptozotocin/High-Fat Diet-Induced Diabetic Rats, Shing-Hwa Liu, Wan-Hsin Wu, Huei-Ping Tzeng, Wenchang Chiang, Meng-Tsan Chiang Dec 2023

Dehulled Adlay (Coix Lachryma-Jobi L.) Ameliorates Hepatic Gluconeogenesis And Steatosis In Streptozotocin/High-Fat Diet-Induced Diabetic Rats, Shing-Hwa Liu, Wan-Hsin Wu, Huei-Ping Tzeng, Wenchang Chiang, Meng-Tsan Chiang

Journal of Food and Drug Analysis

Adlay (Coix lachryma-jobi L.) is a traditional Chinese herbal medicine with various biological activities. We investigated the anti-diabetic effects of different parts of adlay seeds, including polished adlay (PA), adlay bran (AB) and dehulled adlay (DA) in a streptozotocin (STZ)/high fat diet (HFD) diabetic rat model (DM). DM rats supplemented with or without PA (43%), AB (3%), or DA (46%) diet for 8 weeks. The plasma glucose and insulin levels and the insulin resistance index (HOMA-IR) were increased in DM group; among the three adlay diets, DA has the best effects attenuating all of these alterations in DM …


Biological And Clinical Significance Of The Age-Rage Axis In The Aggressiveness And Prognosis Of Prostate Cancer, Shih-Hong Khoo, Pei-Ru Wu, Kun-Tu Yeh, Shih-Lan Hsu, Chi-Hao Wu Dec 2023

Biological And Clinical Significance Of The Age-Rage Axis In The Aggressiveness And Prognosis Of Prostate Cancer, Shih-Hong Khoo, Pei-Ru Wu, Kun-Tu Yeh, Shih-Lan Hsu, Chi-Hao Wu

Journal of Food and Drug Analysis

Dietary factors and chronic hyperglycemia are linked to the formation of advanced glycation end products (AGEs) and prostate cancer (PCa) risk. The activation of the receptor for AGEs (RAGE) acts as a bridge between various RAGE ligands and certain malignancies. This study showed that the interaction of AGEs and RAGE promoted PCa cell proliferation, invasion, and autophagy-mediated survival in response to chemotherapeutic agents. RAGE-overexpressed PCa cells underwent epithelial-mesenchymal transition and showed increased cancer stem cell-like properties. In mouse xenograft models, RAGE-overexpressed cells showed more substantial tumorigenic capacity than parental cells, whereas RAGE knockdown decreased tumorigenicity. The clinical data validated a …


Steamed Daylily Flower (Hemerocallis Fulva L.) Protected Cardiac And Hepatic Cells Against Ethanol Induced Apoptotic And Oxidative Damage, Sheng-Lei Yan, Wen-Tzu Wu, Mei-Chin Mong, Mei-Chin Yin Dec 2023

Steamed Daylily Flower (Hemerocallis Fulva L.) Protected Cardiac And Hepatic Cells Against Ethanol Induced Apoptotic And Oxidative Damage, Sheng-Lei Yan, Wen-Tzu Wu, Mei-Chin Mong, Mei-Chin Yin

Journal of Food and Drug Analysis

Our previous study examined the phytochemical composition and bio-activities of raw daylily flower (Hemerocallis fulva L.). However, this plant food is usually served via heat process such as cooking in a soup. This study aimed to investigate the phytochemical profile and biofunctions of steamed daylily flower (SDF). The content of total phenolic acids, total flavonoids, total carotenoids, total anthocyanins and total triterpenoids in SDF aqueous extract was assessed. Normal cardiac and hepatic cells, H9c2 and L-02 cells, were used to evaluate the protective effects of SDF against ethanol. SDF concentrations of 0.25%, 0.5%, and 1% were applied to treat …


Analysis And Identification Of Phenolic Compounds With Antiproliferative Activity From Chinese Olive (Canarium Album L.) Fruit Extract By Hplc-Dad-Spe-Tt-Nmr, Yu-Te Yeh, Chien-Kuang Chen, Yi-Chun Liao, Shoei-Sheng Lee, Shu-Chen Hsieh Dec 2023

Analysis And Identification Of Phenolic Compounds With Antiproliferative Activity From Chinese Olive (Canarium Album L.) Fruit Extract By Hplc-Dad-Spe-Tt-Nmr, Yu-Te Yeh, Chien-Kuang Chen, Yi-Chun Liao, Shoei-Sheng Lee, Shu-Chen Hsieh

Journal of Food and Drug Analysis

Chinese olives (Canarium album L.) are rich in phenolic compounds, exhibiting a broad spectrum of potential clinical applications. This study is the first report on the isolation and elucidation of bioactive compounds with high antiproliferative activity from the ethyl acetate fraction of a Chinese olive fruit methanolic extract (CO-EtOAc). We used the WST-1 assay to determine which subfractions of CO-EtOAc had significant antiproliferative activity using the murine colon cancer cell line CT26. Subsequently, the functional compounds were characterized by the hyphenated technique and high-performance liquid chromatography-diode array detector-solid phase extraction-transfer tube-nuclear magnetic resonance (HPLC-DAD-SPE-TT-NMR). Thirteen phenolic constituents were identified …


Glycine-Rich Peptides From Fermented Chenopodium Formosanum Sprout As An Antioxidant To Modulate The Oxidative Stress, Chen-Che Hsieh, Shu-Han Yu, Hsing-Chun Kuo, Darin Khumsupan, Hsiao-Chu Huang, Yu-Wei Liou, Chen-Yu Kao, Szu-Chuan Shen, Kuan-Chen Cheng Dec 2023

Glycine-Rich Peptides From Fermented Chenopodium Formosanum Sprout As An Antioxidant To Modulate The Oxidative Stress, Chen-Che Hsieh, Shu-Han Yu, Hsing-Chun Kuo, Darin Khumsupan, Hsiao-Chu Huang, Yu-Wei Liou, Chen-Yu Kao, Szu-Chuan Shen, Kuan-Chen Cheng

Journal of Food and Drug Analysis

Rhizopus oligosporus was utilized in the solid-state fermentation of Chenopodium formosanum sprouts (FCS) in a bioreactor. Subsequently, the antioxidant activity of food proteins derived from FCS was investigated. Results showed that glycine-rich peptide (GGGGGKP, G-rich peptide), identified from the < 2 kDa FCS proteins, had antioxidant values. According to SwissADME, AllerTOP, ToxinPred, and BIOPEP-UWM analyses, G-rich peptide was identified as safe, non-toxic, and non-allergenic. Afterward, the peptide was examined using in silico and in vitro studies to evaluate its potential alleviating oxidative stress caused by particulate matter. This study proposed plausible mechanisms that involve the binding of G-rich peptide which inhibited phosphorylation of the v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) subunit on NF-κB pathway. The inhibition then resulted in down regulation of NF-κB transcription and genetic expression of inflammatory …


Investigation Of Microwave Extraction Of Red Cabbage And Its Neurotherapeutic Potential, Halil Koyu, Serdar Demir, Mehmet Zeki Haznedaroglu Dec 2023

Investigation Of Microwave Extraction Of Red Cabbage And Its Neurotherapeutic Potential, Halil Koyu, Serdar Demir, Mehmet Zeki Haznedaroglu

Journal of Food and Drug Analysis

In the pursuit of bioactive compounds with health benefits from food and medicinal plants, optimization of process to reduce solvent, raw material, energy and time consumption has been a valuable research objective. Sustainable management and efficient use of natural resources are of critical importance for sustainability goals. In this manner, microwave extraction of red cabbage which has been a mass cultivated plant for food purposes, was optimized for maximizing acetylcholinesterase, butyrylcholinesterase, tyrosinase inhibitory activity and bioactive content. Quantitative analysis of total phenol, flavonoid and anthocyanin content were performed using spectrophotometry and UPLC-DAD-ESI-MS/MS. Optimum conditions were determined as 600 W, 100% …


Abundance Of Prevotella Copri In Gut Microbiota Is Inversely Related To A Healthy Diet In Patients With Type 2 Diabetes, Chih-Yiu Tsai, Po-Yu Liu, Meng-Chuan Huang, Chiao-I Chang, Hsin-Yun Chen, Yu-Hsien Chou, Chi-Neu Tsai, Chia-Hung Lin Dec 2023

Abundance Of Prevotella Copri In Gut Microbiota Is Inversely Related To A Healthy Diet In Patients With Type 2 Diabetes, Chih-Yiu Tsai, Po-Yu Liu, Meng-Chuan Huang, Chiao-I Chang, Hsin-Yun Chen, Yu-Hsien Chou, Chi-Neu Tsai, Chia-Hung Lin

Journal of Food and Drug Analysis

While the gut microbiota is known to be influenced by habitual food intake, this relationship is seldom explored in type 2 diabetes patients. This study aims to investigate the relationship between dietary patterns and gut microbial species abundance in 113 type 2 diabetes patients (mean age, 58 years; body mass index, 29.1; glycohemoglobin [HbA1c], 8.1%). We analyzed the gut microbiota using 16S amplicon sequencing, and all patients were catogorized into either the Bacteroides enterotype (57.5%, n = 65) or the Prevotella enterotype (42.5%, n = 48) using the partitioning around medoids clustering algorithm, based on the most representative genera. Patients …


Therapeutic Effects Of Orally Administration Of Viable And Inactivated Probiotic Strains Against Murine Urinary Tract Infection, Vo Thi Hong Van, Zhen-Shu Liu, Yueh Jen Hsieh, Wei-Chen Shiu, Bo-Yuan Chen, Yu-We Ku, Po-Wen Chen Dec 2023

Therapeutic Effects Of Orally Administration Of Viable And Inactivated Probiotic Strains Against Murine Urinary Tract Infection, Vo Thi Hong Van, Zhen-Shu Liu, Yueh Jen Hsieh, Wei-Chen Shiu, Bo-Yuan Chen, Yu-We Ku, Po-Wen Chen

Journal of Food and Drug Analysis

Urinary tract infections (UTIs) are highly prevalent bacterial infections that pose significant health risks. Specific probiotic strains have been recommended for UTI control and management of antibiotic resistance. Otherwise, paraprobiotics, defined as inactivated probiotic cells, offer potential advantages by minimizing risks associated with live microorganisms. However, the effectiveness of heat-killed probiotic strains against UTIs remains uncertain. Additionally, lactoferrin (LF), an iron-binding glycoprotein, exhibits immunomodulatory, antimicrobial, and anti-inflammatory properties. Recently, we had developed recombinant LF-expression probiotics, which can display considerate antibacterial activities against select food-borne pathogens in vitro. Thus, the present study aimed to evaluate the antibacterial activities of heat-killed natural …


Recent Advances In Combined Avant-Garde Technologies (Thermal-Thermal, Non-Thermal-Non-Thermal, And Thermal-Non-Thermal Matrix) To Extract Polyphenols From Agro Byproducts, Isaac Duah Boateng Dec 2023

Recent Advances In Combined Avant-Garde Technologies (Thermal-Thermal, Non-Thermal-Non-Thermal, And Thermal-Non-Thermal Matrix) To Extract Polyphenols From Agro Byproducts, Isaac Duah Boateng

Journal of Food and Drug Analysis

Because food byproducts (waste) are rich in phytoconstituents, valorizing them is crucial for global food security. However, conventional extraction (CE), including decoction, maceration, Soxhlet, etc., for agro byproducts’ polyphenol extraction are time-consuming and rely significantly on vast volumes of potentially aggressive solvents. Hence, Avant-garde extraction technologies, including non-thermal (high hydrostatic pressure (HHPE), pulsed-electric field (PEF), high voltage electrical discharges (HVED), etc.) and thermal extraction (supercritical fluid (SCF), subcritical water extraction (SWE), microwave-assisted extraction (MAE), etc.), as well as their thermal combinations (SCF-PLE, SCCO2-SWE, SCCO2-MAE, etc.), non-thermal combinations (HHPE+UAE, PEF+UAE, HVED+UAE, etc.) and combined thermal-non-thermal (MAE-UAE, etc.) are increasingly replacing CE. …


A Scoping Review Of Wrist-Worn Wearables In Education, Noah L. Schroeder, William L. Romine, Sidney E. Kemp Dec 2023

A Scoping Review Of Wrist-Worn Wearables In Education, Noah L. Schroeder, William L. Romine, Sidney E. Kemp

Biological Sciences Faculty Publications

Within the past ten years, the public's use of wearables, such as smartwatches and fitness trackers, has greatly increased. Recently, researchers have published systematic reviews to examine how wearables are used in educational settings. However, not all wearables are alike. Head-mounted wearables and exoskeletons may be viewed as invasive, whereas smartwatches and wristbands are commonly worn by the public. In this scoping review, we systematically review the literature around wrist-worn wearables (WWs) in educational settings. Our systematic search revealed 1,932 unique research items, of which 46 met the inclusion criteria and were analyzed. Our findings reveal the trends in the …