Life Sciences Commons^™

America’S Newest Public Health Crisis: The Dangerous Side Effects Of The United States Supreme Court, Emma Rekate

The Synapse: Intercollegiate science magazine

No abstract provided.

Identification Of A Rickettsial Endosymbiont In A Soft Tick Ornithodoros Turicata Americanus, Lichao Liu, Daniel E. Sonenshine, Hameeda Sultana, Girish Neelakanta

Biological Sciences Faculty Publications

Bacterial endosymbionts are abundantly found in both hard and soft ticks. Occidentia massiliensis, a rickettsial endosymbiont, was first identified in the soft tick Ornithodoros sonrai collected from Senegal and later was identified in a hard tick Africaniella transversale. In this study, we noted the presence of Occidentia species, designated as Occidentia-like species, in a soft tick O. turicata americanus. Sequencing and phylogenetic analyses of the two genetic markers, 16S rRNA and groEL confirmed the presence of Occidentia-like species in O. turicata americanus ticks. The Occidentia-like species was noted to be present in all developmental stages …

Radon (222rn) As Tracer For Submarine Groundwater Discharge Investigation—Limitations Of The Approach At Shallow Wind-Exposed Coastal Settings, Michael Schubert, Jan Scholten, Matthias Kreuzburg, Eric Petermann, Mariele Lopes De Paiva, Dennis Kohler, V. Liebetrau, John Rapaglia, Michael Schlüter

Biology Faculty Publications

Mapping radon (222Rn) distribution patterns in the coastal sea is a widely applied method for localizing and quantifying submarine groundwater discharge (SGD). While the literature reports a wide range of successful case studies, methodical problems that might occur in shallow wind-exposed coastal settings are generally neglected. This paper evaluates causes and effects that resulted in a failure of the radon approach at a distinct shallow wind-exposed location in the Baltic Sea. Based on a simple radon mass balance model, we discuss the effect of both wind speed and wind direction as causal for this failure. We show that at coastal …

Vaginal Microbiota Of Recurrent Bacterial Vaginosis Patients Under Oral Metronidazole Therapy: Prognosis Based On Specific Species Of Gardnerella And Other Bacterial Vaginosis Associated Species In Association With The Vaginal Microbiome, Ashomathi Ashok Sudhamathi Mollin

Wayne State University Theses

Bacterial Vaginosis (BV) is a state of vaginal dysbiosis in which BV-associated species displace the typically dominant species of Lactobacillus. This urogenital disease elevates the risk for complications such as pre-term birth, STDs and HIV. Currently, there is no consensus on the etiology of BV. Standard of care treatment is oral metronidazole; however, therapy has high rates of recurrence. Our previous qPCR study of recurrent BV patients (3+ episodes/year) found strong associations of Gardnerella_Gsp07, G. swidsinksii, and G. leopoldii with refractory and recurrent responses, and a robust recovery of Lactobacillus in remission patients. However, these findings were not applicable across …

Distribution Of Toxicity Of Plant Species In The Plant Family Sapindaceae (Sub-Family Hippocastanoideae), Vishvam Mehta

Undergraduate Honors Thesis Projects

Maple leaf toxicosis is a lethal condition in which wilted leaves from the plant genus Acer are ingested by equines, leading to hemolytic anemia and methemoglobinemia. In prior studies, the toxicity of various Acer species was quantified, revealing a wide array of responses in terms of hemolysis and methemoglobin production. The object of this study, therefore, is to determine the toxicity of Acer’s closest relatives within the family Sapindaceae, specifically the four additional genera in the subfamily Hippocastanoideae. The information gained from this study will be used to understand the evolution of the toxins responsible for hemolysis and methemoglobin production. …

Managing Agricultural Nitrogen Losses In Crop Production And Mitigation Of Climate Change Effects, Winnie Ntinyari, Mekonnen Giweta, James Mutegi, Cargele Masso, Joseph P. Gweyi-Onyango

All Peer-Reviewed Publications

Nitrogen (N) losses from cropping systems negatively affect soil quality/fertility, crop yield, and the environment through its contribution to climate change and water pollution. İt possesses oil-water-atmospheric system challenges to both the too much and the too little N application countries. Soil and climatic factors are the critical drivers of N loss from agricultural systems as they influence major processes like nitrification and denitrification. Other N loss pathways include leaching, runoff, and volatilization. Nitrous oxide (N2O) and ammonia (NH3) gases are the two pollutants resulting from N fertilizers in crop production and represent N loss’ significant pathway. Nitrous oxide is …

Seasonal Factors Affecting Sea Turtle Nesting In The Southeastern Caribbean Sea (Gulf Of Paria, Venezuela), Clemente Balladares, Digna T. Rueda-Roa, Diego Rodríguez, Frank E. Muller-Karger, Hector Barrios-Garrido

Marine Science Faculty Publications

The nesting characteristics (number of nests and eggs, time of year, nesting initiation, and nesting length) of leatherback (Dermochelys coriacea) and hawksbill (Eretmochelys imbricata) sea turtles of the southern Caribbean Sea (specifically in the Gulf of Paria in Venezuela), were examined in association with weekly precipitation averages and number of rainy days per week during the period between 2009 and 2018. We hypothesized about the influence of rainfall intensity and patterns as the main abiotic factor for sea turtle nesting. On average, leatherbacks preferred nesting during the drier season of each year (March, April, and May), …

A Comparative Test Of Creative Thinking In Preschool Children And Dolphins, Dawn Melzer, Deirdre Yeater, Madison Bradley, Heather M. Hill, Gonzalo Guerra, Kimberly Salazar, Teresa Bolton, Kathleen M. Dudzinski

Psychology Faculty Publications

Creativity is considered one aspect of intelligence. Including creativity allows for more room for expression (e.g., participants can respond with movement instead of written or verbal responses) than in standard intelligence assessments. The Torrance Tests of Creative Thinking (TTCT; Torrance, 1974) are the leading method of assessing creative abilities in school-aged humans and above. To assess creativity in young humans and nonhuman animals, modifications must be made to facilitate nonverbal responses. In the current study, a cross-species comparison was conducted between preschoolers and bottlenose dolphins to examine responses to a modified creativity task in which both species were trained to …

Human Papillomavirus 16 E2 Regulates Host Cell Pathways Important For Cancer Progression And Treatment Sensitivity Which May Contribute To Cancer Outcomes, Christian Fontan

Theses and Dissertations

Human papillomaviruses (HPVs) are causative agents in around 5% of all cancers, including cervical and oropharyngeal. A feature of HPV cancers is their better clinical outcome compared with non-HPV anatomical counterparts. In turn, the presence of E2 predicts a better clinical outcome in HPV-positive cancers; the reason(s) for the better outcome of E2-positive patients is not fully understood.

Previously, we demonstrated that HPV16 E2 regulates host gene transcription that is relevant to the HPV16 lifecycle in N/Tert-1 cells. One of the genes repressed by E2 and the entire HPV16 genome in N/Tert-1 cells is TWIST1. In these studies, we demonstrate …

Bioinformatic Pipeline For Determining Terminal Repeats In The Human Cytomegalovirus Genome Assembled With Pacbio Long Read Sequences, Ahmed Al Qaffas

Theses and Dissertations

Human Cytomegalovirus (HCMV) is a member of the betaherpesvirinae subfamily of the Herpesvirus family. HCMV infection is common among adults worldwide, with an estimated seroprevalence of 66 to 95%, depending on the geographic region (Zuhair et al., 2019). Although most of the virus genomic content has been studied extensively, the terminal repeating region sequences remain understudied. Two main challenges hindered the study of the region: a) limitations of sequencing technologies; and b) misassembly of the repeats due to its complex nature. Here I show a novel bioinformatics pipeline that takes advantage of PacBio's long reads to resolve the challenges mentioned …

Protein Structure And Interaction: The Role Of Aromatic Residues In Protein Structure And Interactions Between Pyridoxine 5'-Phosphate Oxidase/Dopa Decarboxylase, Mohammed H. Al Mughram

Theses and Dissertations

Naturally developed proteins are capable of carrying out a wide variety of molecular functions due to their highly precise three-dimensional structures, which are determined by their genetically encoded sequences of amino acids. A thorough knowledge of protein structures and interactions at the atomic level will enable researchers to get a deep foundational understanding of the molecular interactions and enzymatic processes required for cells, resulting in more effective therapeutic interventions. This dissertation intends to use structural knowledge from solved protein structures for two distinct objectives.

In the first project, we conducted a bioinformatics structural analysis of experimental protein structures using our …

Behavioral Screening And Chiral Bioanalysis Of Emerging Stimulant-Type Drugs In Rats, Tyson R. Baird

Theses and Dissertations

The epidemic of drug use in the United States and elsewhere in the world has resulted in tragic loss of life and substantial economic costs. Novel psychoactive substances (NPS) are one of the contributors to this problem, and the lack of information about many of these drugs compounds their risk. This dissertation proposes a strategy to use intracranial self-stimulation (ICSS) as a behavioral screening tool to assess emerging drugs of abuse for their abuse potential in order to generate a proactive threat assessment. A series of stimulant-type drugs including methcathinone, α-pyrrolidinohexanophenone (α-PHP), cocaine, and the phenyltropane analogs of cocaine WIN35428 …

Identifying The Human Homologs Of Yeast Rab Proteins Ypt10 & Ypt11 And A Global-Scale Louse Endosymbiont Genome Variation, Nathaniel P. Smith

Theses and Dissertations

Amyotrophic lateral sclerosis (ALS) is a late-onset fatal neurodegenerative disease that causes loss of upper and/or lower motor neurons, and currently has no treatment or cure available. Over 90% of cases occur spontaneously with unknown causes, highlighting the complexity of the disease, and only 10% of cases are linked to heritable genetic mutations. Numerous ALS-linked genes are conserved through evolution, and model organisms may therefore provide opportunities to understand disease pathology at a molecular or cellular level, proving instrumental in identifying therapeutic targets. ALS subtype 8 (ALS8) is caused by an autosomal dominant P56S mutation in the VAPB gene that …

Examination Of Common And Rare Variant Genetic Architecture Of Psychiatric Disorders, Mohammad Ahangari

Theses and Dissertations

Psychiatric disorders are often heterogenous in their manifestation and genome-wide association studies have identified many common risk variants involved in their polygenic architectures with varying degrees of pleiotropy. In recent years, large-scale biobanks have also begun sequencing the genome of their participants to elucidate the role of rare risk variation in the genetic architecture of complex phenotypes, including psychiatric traits. This dissertation sought to better understand the role of both common and rare risk variation in the genetic architecture of psychiatric disorders with a particular focus on schizophrenia and alcohol problems. In the first three analyses, we focused on characterizing …

Computational Analysis Of Drug Targets And Prediction Of Protein-Compound Interactions, Sina Ghadermarzi

Theses and Dissertations

Computational prediction of compound-protein interactions generated a substantial amount of interest in the recent years owing to the importance of the knowledge of these interaction for drug discovery and drug repurposing efforts. Research suggests that the currently known drug targets constitute only a fraction of a complete set of drug targets, limiting our ability to identify suitable targets to develop new drugs or to repurpose current drugs for new diseases. These efforts are further thwarted by our limited knowledge of protein-drug (and more generally protein-compound) interactions, where only a subset of drug targets is typically known for the currently used …

Ecological Changes Of The Lower Niobrara River Valley And Lewis And Clark Lake Delta From Past To Present, Madalyn Christine Bollig

Dissertations and Theses

Over half of the world’s large river systems are impacted by dams. These may prevent sediment flow downstream, resulting in accumulation in the upstream reservoir. On the Missouri River, deltas and associated backwater-affected areas occur in nine locations across six reservoirs. One of these, the Lewis and Clark Lake delta-backwater, is influenced by sediment inputs from both the Missouri River and the Niobrara River. While the extension of the prograding delta into the reservoir has been well documented, there has been little research on upstream (backwater) effects of the delta on the lower Niobrara River. This study examined ecological changes …

Evaluating Relative Abundance, Fish Length, And Marine Protected Area Effectiveness For Four Key Rocky Reef Species Along The Northern Californian Coast, Leon Davis

Cal Poly Humboldt theses and projects

Marine protected areas (MPAs) were created with the purpose of helping conserve and restore diminished populations of marine organisms. Measuring the effectiveness of MPAs requires long-term monitoring, investigating the abundance and size distributions of the species that utilize the conservation areas, and comparing the results to neighboring reference sites that are not currently protected. In this study, observations from long-term MPA monitoring in northern California (2010-2019) were modeled with substrate, oceanographic, spatial, temporal, and body size variables to describe the variability in abundance and size of three fish groups: Black rockfish (Sebastes melanops), the Blue rockfish group (comprised …

Northern Elephant Seal (Mirounga Angustirostris) Colony Establishment And Growth In The King Range National Conservation Area, California, Emma Hatton Levy

Cal Poly Humboldt theses and projects

The King Range colony is the northernmost successful and expanding breeding site for northern elephant seals (Mirounga angustirostris). To evaluate the composition and growth of this colony, I conducted systematic surveys recording counts, age classes, births, and resighting seals with individually identifiable flipper tags. The timing of life-history events (breeding, molting, and resting) of all elephant seal sex and age groups at the King Range was consistent with observations at other colonies. Colony counts during breeding and molting have increased steadily over the 4-year study period (2018-2021), and pup production has increased by 87% since the first year …

Drought Responses Across Diverse Conifer Species, Habitats, And Competitive Gradients In Northern California, Gabriel J. Roletti

Cal Poly Humboldt theses and projects

Frequent and intense drought events are rapidly altering stand dynamics in western North American forests. Climate, competition, and site characteristics can affect the growth responses of individual trees to drought stress. The ecological and geographical diversity of northern California provides a unique opportunity to measure these responses across species, habitat types, and levels of competitive pressure. This study used dendrochronological techniques and linear mixed-effects models to assess growth responses to drought in four montane and two coastal conifer species across 54 study sites (nine sites per species and 540 trees total) in northern California. Growth was evaluated from 2002-2018 and …

Variation Of Larval Traits And Copper Tolerance In An Invasive Cryptic Species Complex (Watersipora: Bryozoa), Jason A. Lopiccolo

Cal Poly Humboldt theses and projects

Many factors contribute to the potential of a non-indigenous species to invade an area and become established. For bryozoan colonies of the cryptic species complex Watersipora (Neviani, 1896), this may include larval characteristics such as settlement rate, competency of metamorphosis, swimming duration, and the ability to tolerate copper, a common component in marine anti-fouling paints. Two common groups of Watersipora that occur along the California coast are W. subatra Clade A and an undescribed new species, Clade N. The goal of this research work was to discover what differences, if any, exist in the larval traits and copper tolerances of …

A Characterization Of The Sandy Beach Surf Zone Fish Community And Their Ecology In Northern California And The Effects Of Marine Protected Areas, Katie B. Terhaar

Cal Poly Humboldt theses and projects

Historically written off as dull and homogenous, the dynamics of the sandy beach surf zone remains under studied world-wide. Northern California has been no exception to this global standard, as the sandy beach surf zone ecosystem in this region has yet to be characterized, and the effect of Marine Protected Areas (MPAs) on the fish and macroinvertebrate community fully ascertained.

Considered data-poor by local wildlife officials, commercially, recreationally and culturally important Amphistichus rhodoterus (redtail surfperch) and common Hyperprosopon ellipticum (silver surfperch) utilize the sandy beach surf zone in Northern California. Little is known about the effect of various environmental factors, …

The Effects Of Polymorphisms In The Cx3cr1 Gene On The Development Of Canine Hipdysplasia, Sertaç Atalay, Süleyman Kök

Turkish Journal of Veterinary & Animal Sciences

Hip dysplasia, caused by both environmental and genetic components, is a common disorder characterized by hip instability in humans and dogs. Unfortunately, the genetic mechanisms that cause the disease in both have not been fully determined. The aim of this study was polymorphisms in the exon 2 and 3' UTR regions of the CX3CR1 gene were determined and their effects on the development of Canine Hip Dysplasia (CHD) in three dog breeds (German Shepherd, Belgian Malinois, Labrador Retriever). For this purpose, a case -control study was designed with 172 dogs in Dog Breeding and Training Center (DBTC) in Turkey. Each …

The Apoptotic And Proliferative Effects Of Tulathromycin And Gamithromycin On Bovinetracheal Epithelial Cell Culture, Yaşar Şahi̇n, Ebru Yildirim, Begüm Yurdakök Di̇kmen, Mustafa Türk

Turkish Journal of Veterinary & Animal Sciences

Gamithromycin and tulathromycin are commonly used in the treatment of bovine respiratory bacterial diseases. The current work was undertaken to establish the apoptotic, necrotic, and cytotoxic effects of these antibiotics in the target animal. Cells with apoptosis and necrosis were determined by dual staining method, cytotoxic effects were determined by MTT assay, cell proliferative effects were examined by XCelligence real-time cell analysis system (RTCA-SP). The comparison between gamithromycin and tulathromycin concentrations on tracheal cells in terms of % cell viability was found to be significantly different. While the cell viability percentage of gamithromycin was higher at 150 μg/mL, 180 μg/mL, …

Levels Of Milk Glycosaminoglycans In Holstein Cows With Clinical And Subclinical Mastitis, Nicolas Caggiano, Elisa Crespi, Gonzalo Fiorini, Emilio De Simone

Turkish Journal of Veterinary & Animal Sciences

Mastitis is an inflammation of the mammary gland whose diagnosis is based on clinical criteria and the determination of inflammatory markers. The disease is mainly caused by bacteria causing intra-mammary infections. The etiological agent/s are detected by milk culture or by molecular biology approaches. However, these methodologies are unpractical for on-farm diagnosis, where rapid decisions must be made when infected animals are detected. For this reason, the levels of glycosaminoglycans (GAGs) in milk were evaluated as a tool for the diagnosis of mastitis. GAGs levels were determined by the dimethylmethylene blue assay (DMMB) method in milk from healthy animals and …

Quality Improvement: Implementation Of Individualized Concussion Patient Education And Rehabilitation Treatment Plans, Briana Burgette, Tamara C. Valovich Mcleod

Clinical Practice in Athletic Training

Concussion is a significant health issue at all levels of sports participation. At Saint Luke’s Health System (SLHS) in Kansas City, Missouri, the sports medicine and concussion team discovered an issue with dissemination of patient education for concussion rehabilitation plans. A quality improvement project was launched in August 2019 to develop concussion educational rehabilitation plans, incorporating cluster symptoms into concussion profiles and improving communication during follow-up care. The project promoted improvement of patient understanding of individualized rehabilitation plans and effective communication for the treatment management team. Three cycles of a Plan-Do-Study-Act (PDSA) quality improvement project were completed over 6 months. …

Production Efficiency, Nutrient Digestibility, And Meat Quality Of Broilers Reared Ondifferent Fat Sources And Emulsifiers, Muhammad Shoaib, Shaukat Ali Bhatti, Hassan Iqbal, Shahzad Ashraf, Najam Us Sahar, Muhammad Mahboob Ali Hamid, Muhammad Asim, Mubsher Hussain

Turkish Journal of Veterinary & Animal Sciences

Two fat sources, soybean oil (SO) and refined poultry oil (RPO), and 4 emulsifiers (control, lysolecithin, lysophospholipid, and bile acids) were used in this experiment. Thirteen hundred and sixty (1360) birds (ROSS - 308) were divided into 8 groups having 5 replicates, 34 birds in each. Energy efficiency, feed conversion (FCR), and protein efficiency ratios were improved in birds receiving SO than RPO diets. Weight gain, FCR, feed intake, and dressing percentage were increased in broiler birds receiving bile acid than those given lyso-phospholipid and lysolecithin (p < 0.05). Cooking loss was greater (p < 0.05) in broiler birds given RPO than those fed SO. Birds receiving bile acid had greater (p < 0.05) water holding capacity of chest meat than other fat emulsifiers. Bile acids in broiler diet presented higher (p < 0.05) digestibility of crude protein and ether extract as compared to other treatments. Bile acid supplemented diets had a minimum (p < 0.05) cost of production per kg live weight than other fat emulsifiers. Therefore, the use of bile acids with RPO diets can increase nutrient digestibility, growth performance, meat quality, and economic efficiency in broiler production systems.

Hiring Practices Among Ncaa Division Ii Head Athletic Trainers, B J. Warner, Z K. Winkelmann, S A. Cage, D M. Gallegos, L E. Trail

Clinical Practice in Athletic Training

Context: To the authors’ knowledge, there are few studies on how athletic trainers (ATs) are hired by NCAA institutions. Current legislation protects many demographics from discrimination during hiring, but these laws may not be enforced by institutions. Therefore, the purpose of this study was to describe factors that might influence the hiring practices of NCAA Division II Head Athletic Trainers. Methods: We used a web-based survey (Qualtrics) that was distributed to by email to 278 NCAA Division II Head Athletic Trainers. A total of 96 ATs completed the survey (response and completion rate=34.5%). The survey included a prompt for hiring …

Impact Of A Virtual Education Presentation On Perceived And Actual Knowledge Of Cupping Therapy Among Athletic Trainers, S A. Cage, D L. Gill, P K. Brown, R Schmitz, B J. Warner

Clinical Practice in Athletic Training

Context: Within the athletic training profession, there are continuing education requirements in order to maintain national certification. Although the profession mandates completion of continuing education, little research has assessed the effectiveness of continuing education interventions. Furthermore, there has been even less research conducted on the effectiveness of virtual continuing education interventions. The purpose of this study was to determine the effect of a virtual continuing education presentation on perceived and actual knowledge of cupping therapy among certified athletic trainers. Methods: 31 certified athletic trainers began this study (age = 38 ± 11, years of credentialed experience = 15 ± 11). …

Anisocoria As The Sole Initial Symptom Of Concussion In A Collegiate Volleyball Player: A Case Report, I Hopper, B J. Warner, M Mckenney, R M. Galbraith, C Cox, S A. Cage

Clinical Practice in Athletic Training

Background: A 21-year-old female collegiate volleyball player reported to the athletic training staff after a competition upon noticing that her pupils were unequal. The patient stated that she was not experiencing any other signs or symptoms that would indicate a concussion, but did recall striking her head on the court during pre-competition warmups. The patient reported no previous history of concussion or eye injury. Further evaluation did not reveal any issues with the patient’s reflexes, balance, or memory. At this time, the decision was made to preemptively withhold the patient from participating in athletic activities so she could be monitored …

Productive Performance, Egg Quality, And Hatching Traits Of Japanese Quail Lines Selectedfor Higher Body Weight And Egg Number, Abdur Rehman, Jibran Hussain, Athar Mahmud, Khalid Javed, Abdul Ghayas, Sohail Ahmad

Turkish Journal of Veterinary & Animal Sciences

The present study evaluated the effect of selection for higher body weight (weight based selection = WBS) and egg numbers (egg based selection = EBS) on productivity, egg quality, and hatching traits of Japanese quail for three generations. From a base population of 1125 day-old chicks of Japanese quail, best performing families were allowed to propagate for getting next generation. WBS in G3 presented higher values of feed intake, egg weight, hatching weight. However, egg production and feed conversion ratio were better in EBS during G3. The incidence of embryonic mortality was also lower in the WBS line. In the …