Life Sciences Commons^™

The Role Of Glycogen Synthase Kinase 3 Β (Gsk-3Β) At The Cardiac Myofilament, Marisa Jean Stachowski

Dissertations

Altered kinase activity and localization is gaining appreciation as a mechanism contributing to cardiovascular disease. Previous work suggests that the kinase glycogen synthase kinase 3 β (GSK-3β) regulates contractile function of the myofilament, but this work was performed in vitro. The goal of this work was to investigate GSK-3β’s in vivo role in regulating myofilament function, the mechanisms involved, and the translational relevance in disease. GSK-3β’s myofilament localization was confirmed in wild type mouse and human tissue via western blot of myofilament enriched tissue and immunofluorescence. These experiments also revealed that GSK-3β phosphorylated at Y216 binds to the z-disc. We …

Casting And Splinting Options For Pseudo-Jones Avulsion Fractures: An Evidence-To-Practice Review, Amber M. Kingsley, Aysha B. Reyes, Zachary K. Winkelmann

Clinical Practice in Athletic Training

Avulsion fractures of the fifth metatarsal bone occur when a portion of the bone is broken or pulled off the rest of the bone. These fractures are often seen in athletes, especially those of a young age. Avulsion fractures of the fifth metatarsal bone are commonly treated non-surgically with immobilization of the foot. There are several different options for immobilization of the foot with no clear result as to which immobilization method is the most effective. The purpose of this evidence-to-practice review was to summarize a systematic review on comparative outcomes of immobilization interventions on avulsion fractures of the fifth …

Ars Moriendi: An Overview Of Approaches To The Art Of Dying, Grief And Loss For Nurses Working In Mental Health, Meagan G.A. Dickerson, Darren Conlon, Toby Raeburn

Nursing Papers and Journal Articles

This historical discussion paper is an overview for nurses working in mental health of medieval and Early Modern texts known as Ars Moriendi literature, which focuses on the art of dying, grief and loss. Primary and secondary historical documents are used to describe Ars Moriendi literature and how historical understandings of death and dying were shaped within a spiritual context. Ars Moriendi concepts are compared with modern Western secularised and medicalised notions to prompt reflection on historical versus modern approaches to dying, grief and loss.

Evolution Of Life-History Characteristics In Gadoidei, Joshua Hittie

Master's Theses

Life-history characteristics (e.g., age and growth) have been used extensively to understand the temporal population dynamics of fish species, but less so within a phylogenetic framework. This study investigates life-history characteristics within the suborder Gadoidei (order: Gadiformes) and to test the extent of phylogenetic signal for those characteristics. To accomplish this, a phylogeny of Gadoidei was first constructed based on both mitochondrial and nuclear genes. Within this phylogenetic framework, life-history traits, including growth rate, age at maturity, and longevity, as well as ecological data, such as water depth and diet type, were mapped to the phylogeny using parsimony analysis to …

Phenotypic Analysis Of Murine Gut Associated Lymphocytes, Madison Flood

Master's Theses

γδ T cells are important in the role of immune homeostasis, especially in relation to the gut. It has been shown that there is a higher proportion of γδ T cells in the intestines than in circulation. With this knowledge, our lab focused on understanding the mechanisms of these cells, specifically regarding immune tolerance. In our preliminary research, we identified a subset of γδ T cells that express Foxp3, a transcription factor essential for the functioning of regulatory T cells, in the neonatal (5 day old) intestine. We hypothesized that these Foxp3+ γδ T cells have immunosuppressive functions upon exposure …

Bisacodyl Limits Chikungunya Virus In Vitro And Is Broadly Antiviral, Natalie June Lomascolo

Master's Theses

Identifying novel antivirals requires significant time and resource investment, and the continuous threat of viruses to human health necessitates commitment to antiviral identification and development. Developing antivirals requires years of research and validation, and recent outbreaks have highlighted the need for preparedness in counteracting pandemics. One way to facilitate development is to repurpose molecules already used clinically. By screening such compounds, we can accelerate antiviral development. Here, we screened compounds from the National Institutes of Health’s Developmental Therapeutic Program for activity against chikungunya virus, an alphavirus that is responsible for a significant outbreak in the Americas in 2013. Using this …

Comparison Of Feeding Functional Morphology Development In North American Esocids, Cheryl Theile

Master's Theses

Esocidae is a family of freshwater fishes within order Esociformes known for its long, cylindrical bodies, duck-bill snouts, and an impressive number of sharp teeth. There are four species, including two subspecies, in North America with areas of overlap of their geographic distribution. These ambush predators change diets from invertebrate prey to vertebrate prey during their first year of life, and the timing of this shift varies between species. In particular, the sister species Esox lucius (Northern Pike) and Esox masquinongy (Muskellunge) overlap in the upper Midwest, but E. masquinongy transitions to a vertebrate diet sooner in its development than …

Calcium-Mediated Induction Of Vibrio Fischeri Es114 Biofilms, Katia Elizabeth Semenchuk

Master's Theses

Vibrio fischeri bacteria form biofilms that facilitate symbiotic colonization of the Hawaiian bobtail squid, Euprymna scolopes. These host-associated biofilms require production of the SYP polysaccharide. However, it has been difficult to observe robust biofilm formation in vitro in a laboratory setting using wild-type strain ES114; instead, most work investigating syp-dependent biofilm formation has relied on genetically altered strains. Guided by recent findings that increasing calcium induces c-di-GMP (Tischler et al., 2021), a molecule known in other systems to promote biofilm formation, the question was posed as to whether high levels of calcium alone could promote syp-dependent biofilm formation by ES114. …

Biofilms On Plastic Litter: Community Composition And Activity And The Effects On Ecosystem Processes, Raul F. Lazcano

Master's Theses

The rapid increase of plastic production and disposal has resulted in plastic pollution becoming a global problem. In aquatic ecosystems, plastic litter is a substrate for biofilms, but little research has simultaneously assessed the effects of plastic litter on biofilm activity, community composition, and ecosystem processes. Our objectives were to: (1) measure biofilm activity and community composition on plastic litter relative to a natural surface in an urban river and (2) assess the impact of microplastic pollution on ecosystem metabolism and N2 flux in an oligotrophic lake. For objective (1) we incubated three common plastics with distinct physical and chemical …

Dna Methylation Patterns Of Line-1 Elements Specific To Hc21p In Cancer Cells, Dametria Jetters

Master's Theses

Long Interspersed Nuclear Elements (LINEs) are retrotransposons. They function by making a cDNA copy, reverse-transcribing it into DNA and reinserting itself back into the genome; thereby, increasing its copy number. Sequencing of the human genome revealed that LINEs (L1) are underrepresented on the short arm of human chromosome 21 (HC21), but it is enriched in full-length copies. A number of previous studies have investigated the DNA methylation patterns of L1s in cancer cells, but these studies are typically done in euchromatic regions. The heterochromatic short arm of HC21 is primarily composed of heterochromatin. However these regions can be targeted for …

Unveiling Bacterial Hitchhikers In The Urobiome, Genevieve Baddoo

Master's Theses

Microbial communities of bacteria, viruses, and fungi form complex interactions in nature. However, our understanding of these communities is limited to those that can be cultured in the lab. Enhanced culture methods have enabled the isolation of numerous fastidious species from urinary tract, definitively proving that the urinary tract of asymptomatic individuals is not sterile. In working with these isolates, our group has observed several instances in which a “purified” isolate actually harbors more than one species. We refer to these additional members as bacterial hitchhikers, a term previously used to describe similar occurrences in soil communities. I hypothesized that …

Can Gdp Be Predictive Of Fertility Rates In Sweden?, Hannah Coleman

Honors Program: Senior Projects (Public)

Fertility rates in a country vary and can be influenced by numerous different aspects of an individual’s life and the society in which they live. One aspect that may influence fertility rates at the national level is the gross domestic product (GDP). GDP is an indicator of a country’s economic productivity. Because fertility is linked to both economic development and individual resources, GDP may be a useful indicator of fertility rates. If so, knowing or predicting GDP could be used to project the fertility rates of a population years into the future. To determine if a reasonable connection between GDP …

Optimization And Standardization Of Dms Chemical Mapping Experiments For Probing Rna 3d Structure, Sarah Brady

Honors Program: Senior Projects (Public)

RNA plays a significant and crucial role in biological processes. The properties of RNA are ultimately responsible for each molecule’s unique function. The configuration and structural integrity of RNA is important for its functionality. Chemical mapping detailed in this thesis can be used to analyze the 3D structure of an RNA sequence. RNA is modified via methylation of adenine and cytosine bases using dimethyl sulfate, then reverse transcribed to DNA, then sequenced. The sites where the bases were methylated become mutations in the DNA sequence, allowing for identification of these locations when analyzing sequencing data. Conditions for modification can be …

Multi-Scale Threat Assessment Of Riverine Ecosystems In The Colorado River Basin, Lise Comte, Julian D. Olden, Stacy Lischka, Brett G. Dickson

Faculty Publications – Biological Sciences

Freshwater ecosystems are facing a deepening biodiversity crisis. Developing robust indicators to assess ecological integrity across large spatial scales and identifying the specific threats and pathways of impairment are thus critically needed if we are to inform freshwater conservation strategies. Here we present the first comprehensive threat assessment across the Colorado River Basin – one of the largest and most endangered river basins in North America – using a spatial framework accounting for the wide range of human activities (land uses, transportation infrastructure, exploitative activities, water withdrawals), pathways (local footprint, overland runoff, upstream cumulative effects), and spatial extent of influence …

Internalization Of Appearance Ideals And Not Religiosity Indirectly Impacts The Relationship Between Acculturation And Disordered Eating Risk In South And Southeastern Asian Women Living In The United States, Sonakshi Negi, Erik M. Benau, Megan Strowger, Anne Claire Grammer, C. Alix Timko

Psychology Faculty Publications

Objective: Studies that examine disordered eating in samples of Asian individuals living in the United States frequently combine all individuals of Asian descent into a single group, which can obscure important differences between groups and their experiences of acculturation. The goal of the present study was to establish the relation of acculturation, internalization of appearance ideals, and religiosity as predicting body dissatisfaction and disordered eating in women of South and Southeast Asian (SSEA) descent.

Method: Women of SSEA descent (N = 112) aged 18–51 years (M = 23.10, SD = 6.4) completed a battery of questionnaires that inquire about these …

Extended Functional Connectivity Of Convergent Structural Alterations Among Individuals With Ptsd: A Neuroimaging Meta-Analysis, Brianna S. Pankey, Michael C. Riedel, Isis Cowan, Jessica E. Bartley, Rosario Pintos Lobo, Lauren D. Hill-Bowen, Taylor Sato, Erica D. Musser, Matthew T. Sutherland, Angela R. Laird

Psychology Faculty Publications

Background: Post-traumatic stress disorder (PTSD) is a debilitating disorder defined by the onset of intrusive, avoidant, negative cognitive or affective, and/or hyperarousal symptoms after witnessing or experiencing a traumatic event. Previous voxel-based morphometry studies have provided insight into structural brain alterations associated with PTSD with notable heterogeneity across these studies. Furthermore, how structural alterations may be associated with brain function, as measured by task-free and task-based functional connectivity, remains to be elucidated.

Methods: Using emergent meta-analytic techniques, we sought to first identify a consensus of structural alterations in PTSD using the anatomical likelihood estimation (ALE) approach. Next, we generated functional …

For Whom The Bell Tolls: Psychopathological And Neurobiological Correlates Of The Dna Methylation Index Of Time-To-Death, Sage E. Hawn, Xiang Zhao, Danielle R. Sullivan, Mark Logue, Dana Fein-Schaffer, William Milberg, Regina Mcglinchey, Mark W. Miller, Erika J. Wolf

Psychology Faculty Publications

Psychopathology is a risk factor for accelerated biological aging and early mortality. We examined associations between broad underlying dimensions of psychopathology (reflecting internalizing and externalizing psychiatric symptoms), PTSD, and age-adjusted GrimAge (“GrimAge residuals”), a DNA methylation biomarker of mortality risk relative to age. We also examined neurobiological correlates of GrimAge residuals, including neurocognitive functioning, blood-based biomarkers (of inflammation, neuropathology, metabolic disease), and cortical thickness. Data from two independent trauma-exposed military cohorts (n = 647 [62.9% male, M_age = 52], n = 434 [90% male, M_age = 32]) were evaluated using linear regression models to test associations between …

Rationale And Design Of An Ecological Momentary Assessment Study Examining Predictors Of Binge Eating Among Sexual Minority And Heterosexual Young Women: Protocol For The Health And Experiences In Real Life (Her Life) Study, Kristin E. Heron, Abby L. Braitman, Charlotte A. Dawson, Cassidy M. Sandoval, Lauren V. Butler, Alicia Moulder, Robin J. Lewis

Psychology Faculty Publications

Background: Previous research has identified health disparities between sexual minority and heterosexual women, including increased rates of obesity and binge eating in sexual minority women. Established predictors of binge eating behavior include negative emotions and sociocultural processes; however, these studies are generally conducted in samples of young women where sexual identity is not known or reported. There is a dearth of research evaluating how sexual minority–specific factors (eg, minority stress and connectedness to the lesbian, gay, bisexual, transgender, and queer community) may affect binge eating in sexual minority women. In addition, no studies have examined these processes in racially diverse …

Investigating Seed Maturation And Mortality: A Mechanism For Post-Fire Regeneration In Non-Serotinous Conifers, Madeleine A. Lopez

Cal Poly Humboldt theses and projects

Climate warming and associated dry conditions are contributing to increased fire frequency, severity, and size, for many regions in western North America. These changes in fire activity have prompted concern over the long-term persistence of some conifer species, specifically those not adapted to withstand high-severity fire. However, regeneration of non-serotinous conifer species is possible if the timing of fire occurs following seed maturation, and within a heat range that seeds can withstand, in a regenerative mechanism termed “facultative serotiny.” To address this mechanism, I determined the timing of conifer seed maturation using viability testing for four California conifer species: ponderosa …

Drought, Tree Mortality, And Regeneration In Northen California, Sophia Lb Lemmo

Cal Poly Humboldt theses and projects

The 2012-2016 California drought was the most severe in the state’s recorded history, contributing to the death of millions of trees. While the effects of this drought on forests are relatively well studied in the central and southern Sierra Nevada, less is known about its effects on the heavily timbered and diverse forests of northern California. Through sampling 54 0.25 ha plots in northern California, this study compared tree mortality and regeneration patterns before, during, and after California’s most recent record-setting drought. This study evaluated 1) the influence of habitat and competitive covariates on mortality and regeneration trends using ridge …

Foraging Behavior Of Honeybees Exposed To Mosquito Insecticides, Samantha Marie Diel

Cal Poly Humboldt theses and projects

The main aim of this study was to quantify the effects of mosquito insecticides on honey bee (Apis mellifera), foraging behavior, explicitly assessing the impacts of adult mosquito control practices on honey bee health. The extent to which honey bees are exposed to pyrethrum, pyrethrins, permethrin, and pyrethroids is unknown, as are their effects on honey bee health and vitality. This study sought to answer this question by exposing honey bees to D-Phenothrin with the addition of Piperonyl butoxide (PBO), one of many synthetic pyrethroids widely used against adult mosquitoes to control mosquito-borne diseases. Honey bees were exposed to …

Does Bisphenol-A, An Estrogen-Like Environmental Toxin, Disrupt Expression Of The Neuronal Chloride Exporter Protein During Early Brain Development?, Mayra S. Mendez

Cal Poly Humboldt theses and projects

Bisphenol A (BPA), a manufactured compound found in consumer products, is known to adversely affect early brain development by disrupting normal estrogen signaling. Recently, BPA was reported to suppress expression of a gene encoding the neuron-specific chloride ion transporter, KCC2. Human and animal studies show suppressing KCC2 can cause neuronal and behavioral hyperactivity. Therefore, some adverse effects of BPA may be due to KCC2 suppression and consequent neuronal hyperactivity. This study aimed to determine whether BPA exposure during brain development alters KCC2 expression. A secondary purpose was to evaluate whether a new transgenic zebrafish line, KCC2:mCitrine,could be used to track …

Winter Sandpiper Communities On Humboldt Bay: High-Tide Roost Use And Habitat Features That Influence Roost Site Selection, Chelsea Polevy

Cal Poly Humboldt theses and projects

Roosts, locations at which birds and other animals come together to rest and preen while remaining vigilant for predators, are a common facet of the nonbreeding biology of many shorebirds. Roosts fall on a continuum from traditional to ephemeral; traditional roosts are used consistently by the same individuals, whereas ephemeral roosts are used inconsistently and may be used only once or a few times. Studies show that roost habitat preference is the result of an individual shorebird’s attempt to optimize the costs and benefits associated with time constraints, energetic demands, and danger risks as they relate to overall fitness.

In …

Characterizing The Relationship Of Lgl1 And Akt In Migration Of Murine Neural Stem And Progenitor Cells, Natalie Pedicino

Cal Poly Humboldt theses and projects

Asymmetric cell division and migration are critical for neural stem cell differentiation and brain development. When these processes are dysregulated in neural progenitor cells (NPCs), developmental defects and diseases like glioma can result. Lgl1 is a tumor suppressor gene that was first characterized in Drosophila neuroblasts (Strand et al., 1994). It is best known for its regulation of asymmetric cell division through its association with the Par complex. The PI3K/AKT signaling cascade is involved in cellular migration and is also regulated by Par signaling. Unpublished data from the Sprowles laboratory suggests a potential role of Lgl1 in migration and other …

Investigating The Genetic Diversity Of Immune Genes In Non-Native Populations Of American Bullfrogs (Rana Catesbeiana), Luisa Nereyda Segovia

Cal Poly Humboldt theses and projects

The American Bullfrog (Rana catesbeiana) is a highly invasive species that has successfully colonized habitats around the world. The genetic variability of immune genes within invasive populations, like that of bullfrogs, may contribute to how resistant a population is to pathogens. The objective of this project was to characterize the genetic diversity of an immune gene in invasive bullfrog populations in California to better understand how persistent these populations might be over time. To characterize immune gene variability, I isolated exon 2 of the Major Histocompatibility Complex (MHC) Class II Beta chain gene and a neutral mitochondrial marker, …

Degradation And Nitrogen Cycling In The Context Of Biodiversity-Ecosystem Function Relationships In The Inquiline Bacterial Community Of Darlingtonia Californica, Megan Teigen

Cal Poly Humboldt theses and projects

Biodiversity-ecosystem function (BEF) research aims to explain how species and their environments interact with each other. Microbial communities engage in vital biogeochemical pathways in a variety of natural ecosystems, and yet there are large knowledge gaps about the specific metabolic pathways in which they are involved. Degradation specifically contributes to nitrogen cycling globally through the breakdown of large organic nitrogen compounds into small inorganic nitrogen that is necessary for the survival of many other organisms. In this study, I focused on the degradative function of the inquiline microbial communities found within the carnivorous pitcher plant, Darlingtonia californica. Darlingtonia grows in …

A Preliminary Study For The Development Of Free Weight Back Squat Muscular Fitness Reference Norms For Adults Aged 19-29, Eden G. Marquez

Cal Poly Humboldt theses and projects

The barbell back squat is a simple and common resistance training exercise that is used to evaluate muscular fitness. Its use as an evaluative method for muscular fitness in the general population is limited due to a lack of published reference values for this free weight exercise in the general population. This study presents reference values for the back squat muscular fitness in healthy active young adults (aged 19-29). This data is made of 399 subjects (51% male/49% female). Mean ±SD for participants in anthropometric data were 23.1±2.8 years of age, 73.0±12.9 kg weight, 170.6±9.4 cm height, and 25.0±3.4 body …

Escherichia Coli And Salmonella Sp. In Domestic Cattle And Wild Roosevelt Elk: Fecal Pathogens At The Wildlife-Domestic Interface, Emily A. Buck

Cal Poly Humboldt theses and projects

Direct or indirect contact between domestic populations of animals and wildlife carries an inherent risk for transmission of pathogens that cause infectious disease. In Humboldt and Del Norte counties of northern California, ongoing conflict between ranchers and Roosevelt elk groups results from elk use of ranching pastures and pastures on private land. Fecal samples from elk in association with cattle, cattle, and from elk not in known association with cattle were assessed for the presence of bacteria Salmonella enterica and pathogenic Escherichia coli to assess whether association with cattle increases risk of infection for elk. Group identity (one of the …

Demographic Modeling Of Conservation Strategies For The Yosemite Toad (Anaxyrus Canorus), Corrina Rebecca Kamoroff

Cal Poly Humboldt theses and projects

The Yosemite toad (Anaxyrus canorus) is an anuran species endemic to the Sierra Nevada in California that, like many amphibians globally, has suffered population declines. The documented decline in A. canorus populations across their historic range highlights the need for an effective management strategy to protect the species from future extirpation. For this study, I estimated survival rates of A. canorus using a Cormack-Jolly-Seber model populated with data from a demographic study. I then used a female-only post-birth pulse stochastic Lefkovitch matrix model using vital rates I estimated and from the literature to simulate the effect of different …

Prevalence Of Rickettsia Sp. Phylotype G022 And Rickettsia Tillamookensis In Ixodes Pacificus Nymphs From Northern California, Erin Rose Trent

Cal Poly Humboldt theses and projects

Back in 2011 an unknown Rickettsia species was discovered in adult Ixodes pacificus and became known as Rickettsiaspecies phylotype G022. Preliminary analyses placed it among the Spotted Fever Group Rickettsia, indicating unknown pathogenicity. Another Rickettsia species previously found in I. pacificus, R. tillamookensis, was only recently the subject of research efforts after its original discovery in the 1960s. The main focus of this research was to determine the prevalence of phylotype G022 in nymphal I. pacificus to gain further insight into this tick-Rickettsia relationship. The prevalence of R. tillamookensis was also a topic of this …