Life Sciences Commons^™

Relationships Between Different Management Practices And Selected Soil Health Indicators, Erika Lambert, Jack Howard, Sammuel Tapp

Scholars Week

Relationships Between Different Management Practices and Selected Soil Health Indicators

Jack Howard, Erika Lambert, Samuel Tapp, Corey Hale, Mallorie Snider, Dr. Brian Parr, and Dr. Iin Handayani

Hutson School of Agriculture, Murray State University

Abstract

Soil health is essential for growing crops productively. Healthy soils help to reduce erosion, improve nutrient cycling, and lower input cost. Understanding how crop rotations and tillage systems affect soil is key to preserving soil while also improving agricultural income and efficiency. This study was conducted in Calloway County, Kentucky to observe the effects of different management practices over nine sites of management on the …

The Impacts Of Land Management Practices On Soil Organic Carbon And Soil Physical Properties, Kinsey Hamby, Raelee Story, Dallas Patey

Scholars Week

The Impacts of Land Management Practices on Soil Organic Carbon and Soil Physical Properties

K. Hamby, R. Story, D. Patey, C. Hale, I. P. Handayani, B. Parr, and M. Snider

Hutson School of Agriculture, Murray State University

ABSTRACT

To understand how to care for our soils, an understanding of soil physical properties must first be established. Soil organic carbon (SOC) refers to the carbon component of the organic compounds found in soil organic matter (SOM). SOC can be an indicator of soil structure, tilth, aeration, drainage, and stability. Soil pH is the measure of hydrogen ions suspended in a soil …

A Gud Toolbox: Implementation Of Giving-Up Densities With Mammals, Brandon Preston, Jordan Tandy, Kundil Patel, Karissa Coffield

Scholars Week

Giving-up densities are the density of food remaining within an artificial foraging patch over a specified time and are an indicator of how an organism perceives its environment. The GUD method is particularly useful to study the foraging behavior of an organism relative to predation risk and interactions with other environmental variables (e.g., cover, conspecifics, and food quantity and quality) to address larger ecological and evolutionary questions. The GUD methodology is commonly used with the mammalian taxonomic group due to their general size, detectability, and abundance in comparison to other taxa. However, development of protocols that maximize the potential for …

Mapping Fetch And Diel Movements Of Silver Carp Hypophthalmichthys Molitrix Within Kentucky Lake And Lake Barkley With Gis, Levi Umland

Scholars Week

Title: Mapping Silver Carp movements, relative exposure, and fetch within inland reservoirs using Geographic Information Systems.

Author: Levi G Umland

Invasive species across our country have continued to threaten our native species, compete for habitat via interspecific competition, and have caused economic impacts. Silver Carp (Hypophthalmichthys molitrix), commonly referred to as Asian Carp, have remained in the invasive species spotlight throughout the southern and mid-western states as they have continued to spread. Silver Carp have successfully spread through our rivers and reservoirs, but little is known about Silver Carp movement rates/behavior within reservoirs due to their lower abundance within …

The Effects Of Pelleted Cannabidiol Supplementation On Heart Rate And Reaction Scores In Horses, Anna L. Draeger, Evan P. Thomas, Kiara A. Jones, Shea Porr

Faculty & Staff Research and Creative Activity

The potential use of cannabidiol (CBD) as a nutraceutical to support improved health and welfare has been of increasing interest. In particular, CBD has been shown to decrease anxiety in humans and small animals. While there is little research published on the effects of CBD supplementation in horses, its use is increasing rapidly. The objective of this study was to determine the effect of feeding a pelleted CBD supplement on equine reactivity and heart rate (HR). Seventeen stock-type geldings were divided into control (CON) or treatment (TRT) groups. The TRT group received 100 mg of CBD once daily. Control horses …

Intein Inhibitors As Novel Antimicrobials: Protein Splicing In Human Pathogens, Screening Methods, And Off-Target Considerations, Diana A. Wall, Chunyu Wang, Kenneth Mills, Christopher W. Lennon

Faculty & Staff Research and Creative Activity

Protein splicing is a post-translational process by which an intervening polypeptide, or intein, catalyzes its own removal from the flanking polypeptides, or exteins, concomitant with extein ligation. Although inteins are highly abundant in the microbial world, including within several human pathogens, they are absent in the genomes of metazoans. As protein splicing is required to permit function of essential proteins within pathogens, inteins represent attractive antimicrobial targets. Here we review key proteins interrupted by inteins in pathogenic mycobacteria and fungi, exciting discoveries that provide proof of concept that intein activity can be inhibited and that this inhibition has an effect …

Reactive Chlorine Species Reversibly Inhibit Dnab Protein Splicing In Mycobacteria, Christopher Lennon Dr, Daniel Wahl, J. R. Goetz, Joel Weinberger Ii

Faculty & Staff Research and Creative Activity

Intervening proteins, or inteins, are mobile genetic elements that are translated within host polypeptides and removed at the protein level by splicing. In protein splicing, a self-mediated reaction removes the intein, leaving a peptide bond in place. While protein splicing can proceed in the absence of external cofactors, several examples of conditional protein splicing (CPS) have emerged. In CPS, the rate and accuracy of splicing are highly dependent on environmental conditions. Because the activity of the intein-containing host protein is compromised prior to splicing and inteins are highly abundant in the microbial world, CPS represents an emerging form of posttranslational …

Measuring Palatability As A Linear Combination Of Nutrient Levels In Food Items, Jeffrey S. Young

Faculty & Staff Research and Creative Activity

It well known that palatability and nutritional quality of foods and/or diets are viewed as being in tension with one another. While there exist multiple measures of healthiness, there are no such measures for tastiness. This gap limits the degree to which researchers can investigate this tension and its implications for dietary behavior and hence public health and nutrition policy. The scope of future work concerning the dietary behavior of Americans would expand greatly if researchers better understood consumers’ willingness to eat certain foods, which matters as much as recommending those foods for them to eat in the first place. …

Monitoring Protein Splicing Using In-Gel Fluorescence Immediately Following Sds-Page, Joel Weinberger Ii, Christopher W. Lennon

Faculty & Staff Research and Creative Activity

Inteins garner significant interest from both basic and applied researchers due to their unique catalytic abilities. Herein, we describe a protocol for accurately monitoring protein splicing without purification using in-gel fluorescence immediately following Tris-Glycine SDS-PAGE. Following expression in Escherichia coli, cells are lysed by sonication, cell supernatants are separated using Tris- Glycine SDS-PAGE, and superfolder GFP (sfGFP) fluorescence is directly visualized within gels. This method is rapid, with sfGFP immediately imaged following SDS-PAGE without staining. Further, sfGFP can be specifically detected in complex samples such as E. coli cell supernatants, proteins run at expected masses, and all steps can be …

A Comparison Of The Dermal Swab And Waterborne Methods For Measuring Corticosterone Levels In Amphibians For Use In Wetland Condition Assessments, Andrew W. Sisson, Andrea Darracq, J.B. Moon

Student Scholarship & Creative Works

Wetlands are some of the most biodiverse ecosystems in the world but

have been declining in condition across the United States for decades.

Methods such as the National Wetland Condition Assessment, which

focuses on foliage health, nutrient enrichment, chemical contamination,

and surrounding land usage, are used to catalog the condition of those

wetlands that remain. It is unknown if and how these current measures

of condition related to the physiological responses of the organisms that

inhabit these systems. Amphibians can serve as a model organism for

assessing the linkage between organismal health and wetland condition

due to their high species …

Using Bioenergetics To Model The Effects Of Climate Change On Bluntnose Minnows Pimephales Notatus In A Western Kentucky Stream, Christian Slone, Christian Slone

Student Scholarship & Creative Works

Bioenergetics considers a variety of factors like consumption, excretion, and metabolism to quantify the energy use of an organism. A common use of bioenergetics modeling is to help solve fish stock problems. This project looks to develop a bioenergetics model for Bluntnose Minnows Pimephales notatus to help predict effects of climate change on the biodiversity of western Kentucky streams. We used R and NetLogo to create an agent-based bioenergetics model to simulate the size distribution of Bluntnose Minnows under various temperature regimes. Bluntnose Minnow metabolism increases, and consumption decreases with an increase in water temperature. Fish growth seems to be …

We Speak English Here: An Exploratory Study Of Language Barrier Effects In Agriculture, Camryn Clift

Honors College Theses

This thesis investigates the effects of the language barrier between English-speaking H-2A managers and their Spanish-speaking H-2A employees on Kentucky farms with special attention to the insights that can be gleaned from farm managers concerning the intersection of communication, language barriers, and the unique social and cultural environment created by the microcosm of the H-2A program. The project includes a literature review evaluating the currently documented effects of language barriers in various industries as they relate to the language barriers found within agriculture. IRB-approved interviews with farm managers provide original data to evaluate these potential effects, whether positive or negative. …

Using Crispr-Cas9 To Characterize The Role Of Gli-Similar 3 (Glis3) In Insulin Regulation, Pancreatic Development, And Type 2 Diabetes, Lilyanne Grieve

Honors College Theses

The prevalence of type 2 diabetes continues to rise nationally and internationally, impacting millions of people worldwide. Type 2 diabetes results from insulin resistance leading to chronic hyperinsulinemia and dysfunction of the insulin producing β cells of the pancreas. While environmental factors can influence the development of type 2 diabetes, research has shown genetics are also involved. Gli-similar 3 (Glis3), a Krüppel-like zinc finger transcription factor, has been identified as a novel regulator of insulin transcription. Evidence has shown that loss-of-function Glis3 mutations decrease insulin expression, implicating Glis3 in the development of type 2 diabetes. However, the distinct role Glis3 …

The Disparities Of The Marginalized: Focusing Race And Queerness In Science And Medicine, Kearby Stiles

Honors College Theses

The United States is an immensely diverse country in which certain groups have been—and continue to be—marginalized in society because of their differences. Science and healthcare are areas in which marginalized peoples are negatively affected by a society that punishes difference and diversity. This is an immense problem because in biological and medical school education, in clinical research, and medical practices, little attention is given to marginalized populations. In this paper, I focus on the disadvantages faced by people of color, trans, and intersex people. I decided to focus on race because the history and current state of racism in …

Adaptive Plasticity Of Coloration In Response To Environmental Change, Karissa Coffield

Scholars Week

When rapid environmental changes occur, different selective forces can create phenotypic trade-offs in which a trait can provide fitness benefits or costs under different environmental conditions. Amphibians are particularly vulnerable to environmental change, and previous research has revealed that some species will plastically respond to variation in temperature and ultra-violet radiation (UVR) by altering their coloration. Divergent selection on coloration may change with elevation and climate induced shifts in temperature because high temperatures are likely to result in lighter color morphs but as elevation increases, UVR exposure increases leading to the prediction that darker color morphs will be more common. …

Using Modeling To Investigate Factors Driving Avian Diversity In Urban Ecosystems, Clay Bliznick

Scholars Week

Anthropogenic influences have altered global landscapes considerably throughout the past two centuries, resulting in the decline of natural land cover types. Conversely, land cover types such as cropland and urban areas that are derived from human activities have experienced vast expansion. This landscape transition has serious implications for ecosystem services. To mitigate the loss of these services, it is necessary to maintain ecological integrity within these anthropogenically-influenced systems. Being able to support high biodiversity is an indicator of well-functioning ecosystems, thus quantifying biodiversity and assessing its contributing factors can be useful for developing management strategies in artificial environments. Our objective …

Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger

Scholars Week

Life history morph, sex, and body condition are traits that may influence stress within salamander populations because of differences in physiology and environmental conditions. Given widespread declines and the effects chronic stress can have on amphibian health, it is important to understand within-population drivers of stress and how population level variation may influence population viability. Thus, the objective of our study was to assess how corticosterone varies within the Arizona tiger salamander (Ambystoma mavortium nebulosum) population at the Mexican Cut Nature Preserve. We used a non-invasive skin swabbing method to collect baseline and elevated corticosterone from paedomorph (aquatic …

Understanding Relationships Between Stress, Snake Fungal Disease, And Parasitism In Wild Cottonmouth Snake Populations, Emma Fehlker Campbell

Scholars Week

Chronically raised stress levels are known to correlate with decreased immune function in vertebrates, possible leading to increased susceptibility to infections including parasitism and Snake Fungal Disease (SFD). Helminth endoparasites and Hemoparasites are frequently found in wild populations but little is known about their physiological effects. Additionally, parasite infection may have interactive effects with diseases including SFD. To our knowledge few studies have assessed potential interactions between SFD and parasites in snakes. Thus, the objective of our study is to assess the individual and interactive effects of Helminth endoparasites, hemoparasites, and snake fungal disease on cottonmouth physiological stress. We are …

Soil Quality As Affected By Hemp And Grain Production Systems In Western Kentucky, Jack Eaker

Scholars Week

Since 2018, hemp cropping systems have become increasingly popular in Kentucky. However, there is limited data on soil’s behavior/changes under these management practices as compared to corn and soybean cultivation systems. Therefore, the objective of this study was to determine soil characteristics including soil organic matter, bulk density, water holding capacity, macroporosity, and water at field capacity in hemp and grain cropping systems. The thirty undisturbed soil samples were collected from three different hemp fields and a corn, soybean, and a bare field adjacent to these hemp fields. Each field was located at Murray State University West Farm, Calloway County, …

Impacts Of Digestawell Nrg Supplementation On Post Exercise Muscle Soreness In Unconditioned Horses, A Pilot Study, Jessica K. Suagee-Bedore, Yeting Shen, Shea Porr, Ivan D. Girard, Karen Bennett-Winbrush, Ashley L. Wagner

Faculty & Staff Research and Creative Activity

Exercising horses are commonly plagued by muscle fatigue and soreness, which can result in reduced performance ability. In the present study, ten unconditioned horses were fed 200g per day DigestaWell NRG, a commercial dietary supplement containing Yucca schidigera and Trigonella foenum-graecum, two herbs shown in other species to reduce post-exercise muscle pain and soreness. A control, unsupplemented group contained ten horses of similar age, breed, and gender. Horses completed a 50 minutes, ridden standardized exercise test of moderate intensity immediately prior to (Period1) and after 28 days of supplementation (Period2). Muscle soreness and tightness were evaluated 24 hours prior …

Magnesium’S Impact On Cannabis Sativa ‘Baox’ And ‘Suver Haze’ Growth And Cannabinoid Production, Patrick Veazie, Paul Cockson, David Logan, Brian Whipker

Journal of Agricultural Hemp Research

Limited research exists on the fertility needs for industrial hemp (Cannabis sativa) and the impact of fertility on plant growth and cannabinoids. Optimizing floral production for cannabinoid production and especially cannabidiol (CBD) production, is an economic goal for growers. Magnesium (Mg) is an essential nutrient for plant growth and plays many key roles in plant growth and when deficient leads to suboptimal plant growth. Six Mg fertility rates (0.0, 12.5, 25.0, 50.0, 75.0, and 100.0 mg·L^-1) were evaluated to determine the optimal fertility for C. sativa on two High CBD-type cultivars ‘BaOx; and ‘Suver Haze’. Foliar …

A Survey Of Horse Use And Management In Horse Clubs In China: A Pilot Study, Zhen Li Ph.D., Msc, Shea Porr Phd

Steeplechase: An ORCA Student Journal

Rapid development of the Chinese equine industry in the last two decades has resulted in an increased equine population without trained personnel to support industry growth. The purpose of this study was to understand the present profile of horse use in horse clubs in China from the perspective of horse welfare. The survey was distributed to specific personnel in selected Chinese horse clubs, and snowball sampling techniques were used to increase response rates. Of 20 respondents, the oldest club was established in 2002 and the newest in 2018. Fourteen clubs were membership-based, providing services for 40-1000 members and up to …

Pharmacokinetics Of A Single Feeding Of Pelleted Cannabidiol In Horses, Anna L. Draeger, Laura K. Hoffman, Patricia R. Godwin, Amanda J. Davis, Shea A. Porr

Steeplechase: An ORCA Student Journal

Claims about cannabidiol (CBD) supplementation improving health and behavior are extensive, but research is lacking. Some studies have shown decreased anxiety behavior in rats, and increased activity in osteoarthritic dogs supplemented with CBD, but even less research exists on horses. This study monitored pharmacokinetics and short-term safety for 3 CBD dosages. Eighteen Quarter Horse geldings were randomly assigned to 3 treatment groups: 50 mg (TXT1), 100 mg (TXT2), and 250 mg (TXT3). Dosage was derived from manufacturer recommendations and existing literature on other species. Horses were fed a single dose of CBD pellets. Blood was collected pre- and post-treatment at …

An Evaluation Of College Preparedness On Natural Selection Principles Following The Completion Of An Entry-Level Biology Course, Amy Bell

Murray State Theses and Dissertations

Abstract

Evolution education in secondary education has long been a topic of research. The level of knowledge and acceptance of students upon entering college has been studied using various methods; however, no study had provided the perception of preparedness from the student perspective nor had analyzed the individual Natural Selection principles. This study analyzed college freshmen (n=162) in an entry-level BIO 101 course. Participants were given the Conceptual Inventory of Natural Selection Instrument (CINSI) and perception survey questions upon completion of the course. The CINSI data was then analyzed for each of the four Natural Selection principles as well as …

Science Of Climate Change In Agricultural Courses, Iin P. Handayani

Faculty & Staff Research and Creative Activity

Climate change adaptation is required knowledge for students and graduates from colleges of agriculture since recent crop production and food security are influenced by climate change. Thus, understanding the dynamics of climate change is important to support farmers to adapt to future conditions. However, not all students and graduates understand the concept and application of climate change with regard to the dynamics of food production and future food security. While agriculture faces the challenge of climate change adaptation, agricultural courses have not kept pace by incorporating climate change science into the curricula. To address this issue, eleven syllabi in selected …

The Viability Of Selected Potassium Solubilizing Rhizobacteria In A Mixture Of K-Feldspar And Organic Matter As Carrier Material, Diyan Herdiyantoro, Tualar Simarmata, Mieke R. Setiawati, Nenny Nurlaeny, Benny Joy, Mahfud Arifin, J S. Hamdaniand, Iin P. Handayani

Faculty & Staff Research and Creative Activity

The potassium solubilizing biofertilizer based on selected potassium solubilizing bacteria (KSB) can facilitate availability of potassium in primary silicate minerals containing potassium to be absorbed by the plants. The key to successful application of biofertilizer in showing positive effects on inoculated plants is the selection of a carrier material that can guarantee viability of inoculants during shelf life before being applied in the field. The purpose of this study was to determine viability of three selected KSB isolates which were formulated in a mixture of K-feldspar (KF), rice straw compost (RSC), rice husk biochar (RHB), and Aleksandrov liquid medium (ALM) …

The Reconstruction Of Hatchery Creek: Effects On Macroinvertebrate And Fish Community Dynamics, Patrick Vrablik

Murray State Theses and Dissertations

Hatchery Creek is a restored stream in Jamestown, KY that drains Wolf Creek National Fish Hatchery. The previous degraded channel of Hatchery Creek caused large sediment plumes in the Lower Cumberland River and was restored to decrease sediment loss and provide the opportunity for a self-sustaining trout population. I predicted that the increased amount of habitat would increase taxa richness, abundance and biomass of macroinvertebrates. Macroinvertebrates were monitored in three periods; the degraded period, a one-year recovery period, and a restored period using surber and kicknet samples in order to determine biomass, abundance, diversity, taxa richness, and macroinvertebrate biotic index. …

Dirofilaria Immitis Prevalence In Canis Latrans In Kentucky, Melanie Ann Brandon

Murray State Theses and Dissertations

Dirofilaria immitis (canine heartworm) was found in forty-two (42) of the two hundred seventy-five (275) Canis latrans (coyote) necropsied in the state of Kentucky from November 27, 2019 through March 3, 2021. Thirty-five (35) of the positive cases were from western Kentucky region with the other seven spread across the state. With this group of coyotes, one hundred fifty-eight (158) were male and the other one hundred six-teen (116) were female. The estimated age ranged from a pup to senior dogs. A little over forty percent of the dogs were obtained through coyote/predator tournaments; the remaining were acquired from pest …

Males Of The Wolf Spider Rabidosa Rabida Use Two Mechanisms To Stun Females During Copulation, Daniel Schoenberg

Murray State Theses and Dissertations

The differences in energetic input between the sexes required to produce gametes translates to the differences in reproductive behavior and overall mating systems seen in a species. Females generally produce a few energetically and resource expensive eggs and typically choose a high-quality suitor to ensure she has high quality offspring. In contrast, males produce abundant energetically cheap sperm and attempt to fertilize as many eggs as possible in as many females as possible. Both sexes are trying to maximize their inclusive fitness, but the dichotomy of interests can lead to sexual conflict and perhaps extreme or unusual behaviors such as …

Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett

Murray State Theses and Dissertations

Over the past four decades, emergent fungal diseases have been the most devastating relative to species declines and extinctions. While most research has focused on fungal diseases affecting amphibians and bats, less has focused on diseases like snake fungal disease (SFD), caused by Ophidiomyces ophiodiicola (Oo). SFD was first described in 2006 in North America within a Timber rattlesnake (Crotalus horridus) population in New Hampshire. Since then SFD has been documented in 19 US states, one US territory (Puerto Rico), and Europe. SFD causes high mortality in some species, including the Eastern Massasauga rattlesnakes (Sistrurus …