Life Sciences Commons^™

A Comparison Of The Dermal Swab And Waterborne Methods For Measuring Corticosterone Levels In Amphibians For Use In Wetland Condition Assessments, Andrew W. Sisson, Andrea Darracq, J.B. Moon

Student Scholarship & Creative Works

Wetlands are some of the most biodiverse ecosystems in the world but

have been declining in condition across the United States for decades.

Methods such as the National Wetland Condition Assessment, which

focuses on foliage health, nutrient enrichment, chemical contamination,

and surrounding land usage, are used to catalog the condition of those

wetlands that remain. It is unknown if and how these current measures

of condition related to the physiological responses of the organisms that

inhabit these systems. Amphibians can serve as a model organism for

assessing the linkage between organismal health and wetland condition

due to their high species …

Using Bioenergetics To Model The Effects Of Climate Change On Bluntnose Minnows Pimephales Notatus In A Western Kentucky Stream, Christian Slone, Christian Slone

Student Scholarship & Creative Works

Bioenergetics considers a variety of factors like consumption, excretion, and metabolism to quantify the energy use of an organism. A common use of bioenergetics modeling is to help solve fish stock problems. This project looks to develop a bioenergetics model for Bluntnose Minnows Pimephales notatus to help predict effects of climate change on the biodiversity of western Kentucky streams. We used R and NetLogo to create an agent-based bioenergetics model to simulate the size distribution of Bluntnose Minnows under various temperature regimes. Bluntnose Minnow metabolism increases, and consumption decreases with an increase in water temperature. Fish growth seems to be …

We Speak English Here: An Exploratory Study Of Language Barrier Effects In Agriculture, Camryn Clift

Honors College Theses

This thesis investigates the effects of the language barrier between English-speaking H-2A managers and their Spanish-speaking H-2A employees on Kentucky farms with special attention to the insights that can be gleaned from farm managers concerning the intersection of communication, language barriers, and the unique social and cultural environment created by the microcosm of the H-2A program. The project includes a literature review evaluating the currently documented effects of language barriers in various industries as they relate to the language barriers found within agriculture. IRB-approved interviews with farm managers provide original data to evaluate these potential effects, whether positive or negative. …

Using Crispr-Cas9 To Characterize The Role Of Gli-Similar 3 (Glis3) In Insulin Regulation, Pancreatic Development, And Type 2 Diabetes, Lilyanne Grieve

Honors College Theses

The prevalence of type 2 diabetes continues to rise nationally and internationally, impacting millions of people worldwide. Type 2 diabetes results from insulin resistance leading to chronic hyperinsulinemia and dysfunction of the insulin producing β cells of the pancreas. While environmental factors can influence the development of type 2 diabetes, research has shown genetics are also involved. Gli-similar 3 (Glis3), a Krüppel-like zinc finger transcription factor, has been identified as a novel regulator of insulin transcription. Evidence has shown that loss-of-function Glis3 mutations decrease insulin expression, implicating Glis3 in the development of type 2 diabetes. However, the distinct role Glis3 …

The Disparities Of The Marginalized: Focusing Race And Queerness In Science And Medicine, Kearby Stiles

Honors College Theses

The United States is an immensely diverse country in which certain groups have been—and continue to be—marginalized in society because of their differences. Science and healthcare are areas in which marginalized peoples are negatively affected by a society that punishes difference and diversity. This is an immense problem because in biological and medical school education, in clinical research, and medical practices, little attention is given to marginalized populations. In this paper, I focus on the disadvantages faced by people of color, trans, and intersex people. I decided to focus on race because the history and current state of racism in …

An Evaluation Of College Preparedness On Natural Selection Principles Following The Completion Of An Entry-Level Biology Course, Amy Bell

Murray State Theses and Dissertations

Abstract

Evolution education in secondary education has long been a topic of research. The level of knowledge and acceptance of students upon entering college has been studied using various methods; however, no study had provided the perception of preparedness from the student perspective nor had analyzed the individual Natural Selection principles. This study analyzed college freshmen (n=162) in an entry-level BIO 101 course. Participants were given the Conceptual Inventory of Natural Selection Instrument (CINSI) and perception survey questions upon completion of the course. The CINSI data was then analyzed for each of the four Natural Selection principles as well as …

The Reconstruction Of Hatchery Creek: Effects On Macroinvertebrate And Fish Community Dynamics, Patrick Vrablik

Murray State Theses and Dissertations

Hatchery Creek is a restored stream in Jamestown, KY that drains Wolf Creek National Fish Hatchery. The previous degraded channel of Hatchery Creek caused large sediment plumes in the Lower Cumberland River and was restored to decrease sediment loss and provide the opportunity for a self-sustaining trout population. I predicted that the increased amount of habitat would increase taxa richness, abundance and biomass of macroinvertebrates. Macroinvertebrates were monitored in three periods; the degraded period, a one-year recovery period, and a restored period using surber and kicknet samples in order to determine biomass, abundance, diversity, taxa richness, and macroinvertebrate biotic index. …

Dirofilaria Immitis Prevalence In Canis Latrans In Kentucky, Melanie Ann Brandon

Murray State Theses and Dissertations

Dirofilaria immitis (canine heartworm) was found in forty-two (42) of the two hundred seventy-five (275) Canis latrans (coyote) necropsied in the state of Kentucky from November 27, 2019 through March 3, 2021. Thirty-five (35) of the positive cases were from western Kentucky region with the other seven spread across the state. With this group of coyotes, one hundred fifty-eight (158) were male and the other one hundred six-teen (116) were female. The estimated age ranged from a pup to senior dogs. A little over forty percent of the dogs were obtained through coyote/predator tournaments; the remaining were acquired from pest …

Males Of The Wolf Spider Rabidosa Rabida Use Two Mechanisms To Stun Females During Copulation, Daniel Schoenberg

Murray State Theses and Dissertations

The differences in energetic input between the sexes required to produce gametes translates to the differences in reproductive behavior and overall mating systems seen in a species. Females generally produce a few energetically and resource expensive eggs and typically choose a high-quality suitor to ensure she has high quality offspring. In contrast, males produce abundant energetically cheap sperm and attempt to fertilize as many eggs as possible in as many females as possible. Both sexes are trying to maximize their inclusive fitness, but the dichotomy of interests can lead to sexual conflict and perhaps extreme or unusual behaviors such as …

Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett

Murray State Theses and Dissertations

Over the past four decades, emergent fungal diseases have been the most devastating relative to species declines and extinctions. While most research has focused on fungal diseases affecting amphibians and bats, less has focused on diseases like snake fungal disease (SFD), caused by Ophidiomyces ophiodiicola (Oo). SFD was first described in 2006 in North America within a Timber rattlesnake (Crotalus horridus) population in New Hampshire. Since then SFD has been documented in 19 US states, one US territory (Puerto Rico), and Europe. SFD causes high mortality in some species, including the Eastern Massasauga rattlesnakes (Sistrurus …

Work Ethic And Productivity: The Mediating Role Of Leadership, Everly Tapp

Murray State Theses and Dissertations

Employee work ethic and workplace productivity are vital for organizational success. Past research has shown leadership style to be influential on both, with the manager-employee relationship impacting employee’s attitude and output in the workplace. This study investigated the relationship between employee work ethic and workplace productivity, while also examining the impact of transformational, transactional, and laissez-faire leadership styles on agriculture equipment dealership employees. The leadership style of service managers, as measured by the Multifactor Leadership Questionnaire (MLQ) (5x-Short), was compared to the workplace productivity and work ethic scores of the service technicians they manage. Service technician workplace productivity data was …

Evaluating The Effects Of Residual Broiler Litter And Copper In Soil On Hemp (Cannabis Sativa L.) Fertility And Cannabinoid Production, Sarah E. Forden

Murray State Theses and Dissertations

This study encases two separate experiments observing hemp (Cannabis sativa L.) and soil fertility with the use of broiler litter and copper. The long-term broiler litter research plots were established in 1997 and the copper fertility research plots were not established until 2009. Both experiments use a randomized complete lock design with the broiler litter experiment composed of four replications and eight treatments at the following rates: 0, 1, 2, 3, 4, 5, 6, and 7 tons BL * ac^-1. The copper experiment was designed with four replications and three treatments at the following rates: 0, 10, …

Characterizing The Host Usage And Development Of Ormia Lineifrons (Diptera: Tachinidae), Kyler J. Rogers

Murray State Theses and Dissertations

Insect communication systems are strongly driven by the evolution of signals or signal preferences. These systems rely on a signaler to truthfully emit signals for receivers to interpret. Often, these signals are conspicuously broadcasted. Conspicuous signals involved in animal communication are intended to attract mates, however, these signals are often exploited by eavesdroppers. Thus, many communication systems experience natural selection and sexual selection acting in opposite directions. New adaptations can arise in response to selective pressures, such as eavesdroppers, leading to co-evolving systems between eavesdroppers and hosts, for example. Understanding these systems can provide valuable insight into how unintended receivers …

Effectiveness Of The Two Plus Two Online Program In Agriscience Education: A Case Study Of Students’ Experiences And Persistence, Ronald M. Howell Jr

Murray State Theses and Dissertations

Higher education is changing, and more community colleges find themselves helping to increase the number of students who receive a bachelor’s credential from a 4-year post-secondary institution. This qualitative study was designed to determine the effectiveness of the online two plus two Agriscience Education program at a university in North Carolina. Twelve current and former students provided feedback about their perspectives and lived experiences through semi-structured interviews.

Preliminary findings suggest that both current and former students find the two plus two program very cost-affordable, flexible, and convenient for transferring two years’ worth of credit and courses to earn a bachelor’s …

Glial Granules And Germline Proteins In The Drosophila Brain, Samuel J. Tindell

Murray State Theses and Dissertations

My overarching goal was to elucidate the role of Tudor (Tud) protein in the Drosophila brain. Tud is an evolutionarily conserved molecular scaffold, containing 11 Tud domains which bind to symmetrically dimethylated arginine (sDMA) residues in a variety of proteins. Tud is an essential component of embryonic ribonucleoprotein complexes (RNPs) called germ granules that contain RNAs and proteins crucial to the differentiation and maintenance of germ cells, the specialized class of stem cells that ultimately give rise to the next generation of organism. In the absence of Tud, there is a complete abolishment of germ cells highlighting its important role …