Life Sciences Commons^™

Pedia: Prioritization Of Exome Data By Image Analysis., Tzung-Chien Hsieh, Martin A Mensah, Jean T Pantel, Dione Aguilar, Omri Bar, Allan Bayat, Luis Becerra-Solano, Heidi B Bentzen, Saskia Biskup, Oleg Borisov, Oivind Braaten, Claudia Ciaccio, Marie Coutelier, Kirsten Cremer, Magdalena Danyel, Svenja Daschkey, Hilda David Eden, Koenraad Devriendt, Sandra Wilson, Sofia Douzgou, Dejan Đukić, Nadja Ehmke, Christine Fauth, Björn Fischer-Zirnsak, Nicole Fleischer, Heinz Gabriel, Luitgard Graul-Neumann, Karen W Gripp, Yaron Gurovich, Asya Gusina, Nechama Haddad, Nurulhuda Hajjir, Yair Hanani, Jakob Hertzberg, Konstanze Hoertnagel, Janelle Howell, Ivan Ivanovski, Angela Kaindl, Tom Kamphans, Susanne Kamphausen, Catherine Karimov, Hadil Kathom, Anna Keryan, Alexej Knaus, Sebastian Köhler, Uwe Kornak, Alexander Lavrov, Maximilian Leitheiser, Gholson J Lyon, Elisabeth Mangold, Purificación Marín Reina, Antonio Martinez Carrascal, Diana Mitter, Laura Morlan Herrador, Guy Nadav, Markus Nöthen, Alfredo Orrico, Claus-Eric Ott, Kristen Park, Borut Peterlin, Laura Pölsler, Annick Raas-Rothschild, Linda Randolph, Nicole Revencu, Christina Ringmann Fagerberg, Peter N Robinson, Stanislav Rosnev, Sabine Rudnik, Gorazd Rudolf, Ulrich Schatz, Anna Schossig, Max Schubach, Or Shanoon, Eamonn Sheridan, Pola Smirin-Yosef, Malte Spielmann, Eun-Kyung Suk, Yves Sznajer, Christian T Thiel, Gundula Thiel, Alain Verloes, Irena Vrecar, Dagmar Wahl, Ingrid Weber, Korina Winter, Marzena Wiśniewska, Bernd Wollnik, Ming W Yeung, Max Zhao, Na Zhu, Johannes Zschocke, Stefan Mundlos, Denise Horn, Peter M Krawitz

Faculty Research 2019

PURPOSE: Phenotype information is crucial for the interpretation of genomic variants. So far it has only been accessible for bioinformatics workflows after encoding into clinical terms by expert dysmorphologists.

METHODS: Here, we introduce an approach driven by artificial intelligence that uses portrait photographs for the interpretation of clinical exome data. We measured the value added by computer-assisted image analysis to the diagnostic yield on a cohort consisting of 679 individuals with 105 different monogenic disorders. For each case in the cohort we compiled frontal photos, clinical features, and the disease-causing variants, and simulated multiple exomes of different ethnic backgrounds.

RESULTS: …

The Alliance Of Genome Resources: Building A Modern Data Ecosystem For Model Organism Databases., Alliance Of Genome Resources Consortium, Carol J Bult, Judith A. Blake, Brian R Calvi, J. Michael Cherry, Valentina Difrancesco, Robert Fullem, Kevin L Howe, Thom Kaufman, Chris Mungall, Norbert Perrimon, Mary Shimoyama, Paul W Sternberg, Paul Thomas, Monte Westerfield

Faculty Research 2019

Model organisms are essential experimental platforms for discovering gene functions, defining protein and genetic networks, uncovering functional consequences of human genome variation, and for modeling human disease. For decades, researchers who use model organisms have relied on Model Organism Databases (MODs) and the Gene Ontology Consortium (GOC) for expertly curated annotations, and for access to integrated genomic and biological information obtained from the scientific literature and public data archives. Through the development and enforcement of data and semantic standards, these genome resources provide rapid access to the collected knowledge of model organisms in human readable and computation-ready formats that would …

The Mayfly Newsletter, Donna Giberson

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera

2019 Hitchcock Raptor Banding Project In Perspective, Jerry Toll

Nebraska Bird Review

Hitchcock Nature Center in Pottawattamie County, north of Crescent, Iowa, is home to the Hitchcock HawkWatch and Raptor Banding Project. The Nature Center is in the heart of the Loess Hills which, together with the flat expanse of the Missouri River Valley to the west, provides the perfect conditions for creating the updraft that aids migrating birds. The Hawkwatch began in 1992, and every fall from September through December, birds-of-prey enthusiasts gather on the 45-foot observation tower to watch and count thousands of eagles, hawks, and other raptors as they migrate south. Every year since 2007, Sandy Reinken and I …

Recent Observations Of Water Shrews In Northeastern South Dakota, Dennis Skadsen, Robert M. Timm

The Prairie Naturalist

North American water shrews in the genus Sorex are a complex of at least five species, three of which were recognized historically, Sorex alasksans, S. bendirii, and S. palustris (Hall 1981). Within what was previously considered the single, widespread northern species, S. palustris, two additional species are now recognized, S. albibarbis in the eastern US and Canada and S. navigator in the western United States and Canada (Hope et al. 2014; Nagorsen et al. 2017; Woodman 2018). The American water shrew (Sorex palustris) originally was documented in South Dakota by three females, two were collected 1876 and …

Final Thoughts As Editor-In-Chief, Christopher N. Jacques

The Prairie Naturalist

Greetings GPNSS members! I write this editorial during a time of reflection as Editor-in-Chief of The Prairie Naturalist (TPN), and during unprecedented times as the global COVID-19 pandemic continues. In full disclosure, I do not have a particular topic for this editorial, other than to offer a few final thoughts as my time serving the Great Plains Natural Science Society and TPN.

Bats Of The Loess Hills Ecoregion Of Southeast Nebraska, Virgil Brack Jr, Dale W. Sparks, Darwin C. Brack

The Prairie Naturalist

We surveyed bats at 49 sites in the Loess Hills Ecoregion of southeastern Nebraska, along the western edge of the eastern forest biome in eastern Richardson, Nemaha, and Otoe counties. We completed this study shortly before the northern long-eared bat (Myotis septentrionalis) was listed by the United States Fish and Wildlife Service under the Endangered Species Act. The expectation of listing, along with potential presence of the endangered Indiana bat (Myotis sodalis), motivated the study. We captured 183 bats of five species: eastern red bat (Lasiurus borealis) (n = 103; 56 %), big brown …

Forage News [2019-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

No abstract provided.

Examining The Influence Of Skeletal Muscle Oxygen Metabolism On Microvascular Reperfusion In Response To Acute Ischemia, Ryan Phillip Rosenberry

Kinesiology Dissertations - Archive

Cardiovascular disease (CVD) is the single leading cause of morbidity and mortality in the United States (and the world), with a death rate greater than cancer and chronic lower respiratory disease (emphysema, bronchitis, asthma) combined. Therefore, it is critically important to continue developing techniques for assessing cardiovascular disease. Reactive hyperemia (RH), the magnitude of tissue reperfusion following a period of transient ischemia, is a common technique that is conventionally interpreted as an index of microvascular function and has been shown to be a sensitive and powerful predictor of cardiovascular risk factors and future cardiac events. In Chapter 2, we provide …

Pathophysiology Of Exercise Intolerance In Breast Cancer Survivors Treated With Anthracycline Chemotherapy, Rhys I. Beaudry

Kinesiology Dissertations - Archive

Anthracyclines emerged as frontline breast cancer adjuvant therapy in the late 1960’s. Within half a decade of their clinical adoption, dose-limiting cardiotoxicity was recognized and cumulative dose limits were established to acceptably balance anti-tumor and cardiotoxic properties. Despite these longstanding dose limits, anthracycline treated breast cancer (BC) survivors have advanced biological aging, as evidenced by a marked impairment in peak oxygen uptake (VO2 peak). Given that the pathophysiology of reduced VO2 peak in BC is poorly understood, the aim of this dissertation was to: 1) develop a non-invasive imaging technique to investigate cardiac function under exercise stress; 2) investigate the …

Roli Dhe Rëndësia E Kompleksit Të Vitaminave B, Almire Shala

Theses and Dissertations

Vitaminat janë substanca organike, të përhapura në të gjitha ushqimet, si tek bimët, shtazët dhe mikroorganizmat ndërsa njeriu i merr ato nëpërmjet ushqimit. Vitaminat kanë rëndësi të madhe sepse ruajnë dhe mirëmbajnë metabolizmin e organizmit, janë thelbësore për të gjitha funksionet e organizmit përfshirë metabolizmin, rigjenerimin e indeve dhe mirëmbajtjen e shëndetit të njeriut si pjesë aktive e metabolizmit të organizmit. Qëllimi kryesor i punimit ka qenë analizimi i vitaminave B kompleks, roli dhe rëndësisa që kan këto vitamina në organizëm.

Materiali i përdorur për këtë punim është siguruar kryesisht nga interneti, nga faqja me e madhe e publikimeve mjekësore …

Përcaktimi I Baktereve Mezofile Aerobe Si Dhe Koliformeve Në Qumështin E Papërpunuar Në Regjionin E Prizrenit, Suharekës Dhe Rahovecit, Duresa Ukaj

Theses and Dissertations

Cilësia mikrobiologjike e qumështit të papërpunuar është thelbësore për prodhimin cilësor të produkteve të qumështit. Alternimi është një term që përshkruan ndryshimin e përbërjes, shijes dhe erës në ato pika ku është i pangrënshëm për konsumatorin. Alternimi mikrobial i qumështit shpesh përfshin degradimin e proteinave, karbohidrateve dhe yndyrnave të organizmave dhe enzimave të tyre. Konsumimi i qumështit dhe qumështit është rritur në mënyrë të konsiderueshme në Kosovë gjatë dekadës së fundit, dhe një pjesë e madhe e prodhimit vendor vjen nga shpërndarësit në shkallë të vogël në të gjithë vendin. Në këtë hulumtim, janë marrë 50 mostra qumështi në disa …

Distinct Aspects Of The Early Environment Contribute To Associative Memory, Cued Attention, And Memory-Guided Attention: Implications For Academic Achievement, Maya L. Rosen, Andrew N. Meltzoff, Margaret A. Sheridan, Katie A. Mclaughlin

Neuroscience: Faculty Publications

Childhood socioeconomic status (SES) is associated with numerous aspects of cognitive development and disparities in academic achievement. The specific environmental factors that contribute to these disparities remain poorly understood. We used observational methods to characterize three aspects of the early environment that may contribute to SES-related differences in cognitive development: violence exposure, cognitive stimulation, and quality of the physical environment. We evaluated the associations of these environmental characteristics with associative memory, cued attention, and memory-guided attention in a sample of 101 children aged 60–75 months. We further investigated whether these specific cognitive abilities mediated the association between SES and academic …

Polyhistidine Facilitates Direct Membrane Translocation Of Cell-Penetrating Peptides Into Cells, Han-Jung Lee, Yue-Wern Huang, Shiow-Her Chiou, Robert Aronstam

Biological Sciences Faculty Research & Creative Works

The bovine lactoferricin L6 (RRWQWR) has been previously identified as a novel cell-penetrating peptide (CPP) that is able to efficiently internalize into human cells. L6 interacts with quantum dots (QDs) noncovalently to generate stable L6/QD complexes that enter cells by endocytosis. In this study, we demonstrate a modified L6 (HL6; CHHHHHRRWQWRHHHHHC), in which short polyhistidine peptides are introduced into both flanks of L6, has enhanced cell-penetrating ability in human bronchoalveolar carcinoma A549 cells. The mechanism of cellular uptake of HL6/QD complexes is primarily direct membrane translocation rather than endocytosis. Dimethyl sulfoxide (DMSO), but not pyrenebutyrate (PB), ethanol, oleic acid, or …

2019-2020 Kentucky Agricultural Economic Situation And Outlook, Kenneth H. Burdine, Todd D. Davis, Jerry Pierce, William M. Snell, Timothy A. Woods, Jeffrey W. Stringer, Bobby Ammerman

Agricultural Situation and Outlook

No abstract provided.

Plant Community Composition And Structure Monitoring At Wind Cave National Park, 2019 Data Report, Theresa L. Schaffner, Daniel J. Swanson, Stephanie L. Rockwood

United States National Park Service: Publications

Abstract

This report presents the results of the Northern Great Plains Inventory and Monitoring Network (NGPN) and Northern Great Plains Fire Ecology Program (NGPFire) vegetation monitoring at Wind Cave National Park (WICA) in 2019. This was the ninth year of combined monitoring efforts.

Crew members from NGPN visited 18 long-term monitoring plots to collect data representing the plant communities at WICA. The NGPFire program collected pre- and post-burn data from an additional 37 plant community and forest structure monitoring plots. This work is part of a long-term monitoring effort designed to evaluate the condition of the vegetation community and understand …

Vegetation Classification And Mapping, Missouri National Recreational River, Tom Baldvins, Matt Ley, David Jones, Joe Stevens, Hannah Pilkington

United States National Park Service: Publications

Executive Summary

The vegetation inventory project at Missouri National Recreational River (MNRR) classified and mapped vegetation within the park administrative boundary and estimated thematic map accuracy quantitatively. The project was conducted over a four year period from the summer of 2015 to the winter of 2019.

The project follows guidance provided by the National Park Service (NPS) Vegetation Mapping Inventory (VMI) Program. The overall process includes initial planning and scoping, imagery procurement, field data collection, data analysis, imagery interpretation and classification, and accuracy assessment. The initial planning and scoping meetings to support study plan development took place in December, 2009, …

Fragsad: A Database Of Diversity And Species Abundance Distributions From Habitat Fragments, Jonathan M. Chase, Mario Liebergesell, Alban Sagouis, Felix May, Shane A. Blowes, Åke Berg, Enrico Bernard, Berry J. Brosi, Marc W. Cadotte, Luis Cayuela, Adriano G. Chiarello, Jean Francois Cosson, Will Cresswell, Filibus Danjuma Dami, Jens Dauber, Chris R. Dickman, Raphael K. Didham, David P. Edwards, Fábio Z. Farneda, Yoni Gavish, Thiago Gonçalves-Souza, Demetrio Luis Guadagnin, Mickaël Henry, Adrià López-Baucells, Heike Kappes, Ralph Mac Nally, Shiiwua Manu, Alexandre Camargo Martensen, Duncan Mccollin

Biological Sciences Faculty Publications

Habitat destruction is the single greatest anthropogenic threat to biodiversity. Decades of research on this issue have led to the accumulation of hundreds of data sets comparing species assemblages in larger, intact, habitats to smaller, more fragmented, habitats. Despite this, little synthesis or consensus has been achieved, primarily because of non-standardized sampling methodology and analyses of notoriously scale-dependent response variables (i.e., species richness). To be able to compare and contrast the results of habitat fragmentation on species’ assemblages, it is necessary to have the underlying data on species abundances and sampling intensity, so that standardization can be achieved. To accomplish …

Inhibition Of Serine, Glycine, One-Carbon Metabolism Reduces Proinflammatory Response, Ashlynn Mccall

Honor’s College Freeman Research Journal

Acute lung injury affects around 200,000 critically ill patients annually, with a mortality rate near 33%. With a lack of targeted therapies, it is vital to discover and understand the fundamental cellular processes that drive macrophage inflammation. In the field of macrophage immunometabolism, it is accepted that macrophages upregulating glycolysis indicates a role in proinflammatory processes, however, there is a lack of knowledge surrounding the intermediate pathways in glycolysis. This study examined the effects of serine, glycine, one-carbon metabolism inhibition on LPS-induced inflammation in bone marrow-derived macrophages. We show that the enzymatic expression of PHGDH and PSATl is unaffected when …

Effects Of The French Contrast Method On Maximum Strength And Vertical Jumping Performance, Mathew L. Welch, Eric T. Lopatofsky, Jared R. Morris, Christopher B. Taber

Exercise Science Faculty Publications

Introduction: French Contrast Method (FCM) was first created by French track and field coach Gilles Cometti. Anecdotally, FCM is widely utilized by strength and conditioning coaches in their programming for athletes. However, research on the effectiveness of this method is limited. Methods: A pre-post design was used to examine the effects of FCM on maximum strength and vertical jumping performance. A total of 10 weight-trained males volunteered to participate in this study (Age: 21.84±2.38, Height: 175.37±4.94, Body Mass: 80.6±11.11 Training Age: 4.7±2.3, one repetition maximum (1RM) Back Squat: 136.9±37.14, 1RM Trap Bar Deadlift: 189±42.85).

Tuta Absoluta (Lepidoptera: Gelechiidae) Invasion In Nigeria: First Report Of Its Distribution, Pascal Osa Aigbedion-Atalor, Abiola O. Oke, Abiola A. Oladigbolu, Adejumo A. Layade, Igho B. Igbinosa, Samira A. Mohamed

All Peer-Reviewed Publications

The South America tomato pinworm Tuta absoluta is a serious invasive species in Nigeria. Its impacts on crop production are well understood, but its distribution in the country is unclear. Here, between March 2017 and July 2018, mapping surveys of the distribution of T. absoluta were conducted in 528 different locations across 11 states in Nigeria. Results showed that T. absoluta is present and established in all 360 sites surveyed in the northern and south-western parts of Nigeria. However, it was not recorded at all locations surveyed in two states, Edo and Delta, further south of the country. The implications …

The Nutritive Value Of Black Soldier Fly Larvae Reared On Common Organic Waste Streams In Kenya, Marwa Shumo, Isaac M. Osuga, Fathiya M. Khamis, Chrysantus M. Tanga, Komi K.M. Fiaboe, Sevgan Subramanian, Sunday Ekesi, Arnold Van Huis, Christian Borgemeister

All Peer-Reviewed Publications

In Africa, livestock production currently accounts for about 30% of the gross value of agricultural production. However, production is struggling to keep up with the demands of expanding human populations, the rise in urbanization and the associated shifts in diet habits. High costs of feed prevent the livestock sector from thriving and to meet the rising demand. Insects have been identified as potential alternatives to the conventionally used protein sources in livestock feed due to their rich nutrients content and the fact that they can be reared on organic side streams. Substrates derived from organic by-products are suitable for industrial …

Electropermeabilization Does Not Correlate With Plasma Membrane Lipid Oxidation, Olga Michel, Andrei G. Pakhomov, Maura Casciola, Jolanta Saczko, Julita Kulbacka, Olga N. Pakhomova

Bioelectrics Publications

The permeabilized condition of the cell membrane after electroporation can last minutes but the underlying mechanisms remain elusive. Previous studies suggest that lipid peroxidation could be responsible for the lasting leaky state of the membrane. The present study aims to link oxidation within the plasma membrane of live cells to permeabilization by electric pulses. We have introduced a method for the detection of oxidation by ratiometric fluorescence measurements of BODIPY-C11 dye using total internal reflection fluorescence (TIRF) microscopy, limiting the signal to the cell membrane. CHO-K1 cells were cultured on glass coverslips coated with an electroconductive indium tin oxide (ITO) …

Supporting Black Mothers At The Transition: A Collaborative Design Prenatal Coparenting Program, Jordan Ebanaya

Masters Theses

Despite having the highest infant mortality rate of similarly developed nations, infant mortality rates have trended downward in the United States, however, the infant mortality disparity between Black and White infants persists. The causes of the disparity have been traced to social considerations of the mother especially while pregnant. These social situations include racism and discrimination, marital status of the mother, social support and pregnancy intentions, pointing to one commonality: stress. Using Boss and colleagues 2017 Conceptual Model of Family Stress, this study proposes a solution to address some of the social support, or resources, available to the expectant mother; …

Functional Analysis In The Home Setting Of An Older Adult With Neurocognitive Disorder, Emily Norton

Masters Theses

Currently within the behavioral gerontology literature there are no published studies that include a functional assessment in the home setting. The primary goal of the present study was to address this gap by conducting a functional analysis on a challenging behavior of an older adult with neurocognitive disorder in the home. This study occurred in two phases. During phase one, researchers conducted an antecedent functional analysis on the bizarre speech of an 81-year-old female with suspected dementia across four conditions (television on, no interactions initiated; television on, interactions initiated; television off, no interactions initiated; television off, interactions initiated). Results from …

Hepatobiliary Analyses Suggest Chronic Pah Exposure In Hakes (Urophycis Spp.) Following The Deepwater Horizon Oil Spill, Rachel E. Struch, Erin Pulster, Andrea D. Schreier, Steven A. Murawski

C-IMAGE Publications

Prior to the Deepwater Horizon oil spill, we lacked a comprehensive baseline of oil contamination in the Gulf of Mexico's sediments, water column, and biota. Gaps in prespill knowledge limit our ability to determine the aftereffects of the Deepwater Horizon blowout or prepare to mitigate similar impacts during future oil spill disasters. We examined spatiotemporal differences in exposure to and metabolism of polycyclic aromatic hydrocarbons (PAHs) in 2 hake species (Urophycis spp.) to establish a current baseline for these ecologically important, abundant, and at-risk demersal fishes. Gulf hake (Urophycis cirrata) and southern hake (Urophycis floridana) were collected throughout the Gulf …

Physicochemical And Colligative Investigation Of Α (Shrimp Shell)-And Β (Squid Pen)-Chitosan Membranes: Concentration-Gradient-Driven Water Flux And Ion Transport For Salinity Gradient Power Andseparation Process Operations, Clifford R. Merz

Marine Science Faculty Publications

Chitin, and its derivative chitosan, is a naturally occurring biopolymer and an abundant polysaccharide containing acetylated units of N-acetyl-d-glucosamine. Chitosan membranes produced from shrimp shell (α) and squid pen (β) biowaste were prepared by solvent-casting, after which water flux and ionic transport diffusion experiments were conducted using a side-by-side concentration test cell under differing salinity concentration gradients. Physicochemical and experimental investigations were conducted, which confirmed that β-chitin possesses differing and enhanced performance characteristics than α-chitin with respect to diffusive water flux and ionic transport capabilities. In addition, novel colligative investigations through osmotic equilibrium were conducted to determine electrochemical characteristics …

Bowles, Hunter Joe, B. 1996 (Fa 1384), Manuscripts & Folklife Archives

Folklife Archives Finding Aids

Finding aid only for Folklife Archives Project 1384. Interviews with honey and mushroom producers in southcentral Kentucky, conducted by Hunter Bowles in fall 2019 for a WKU folk studies class in foodways.

How Conflict Shapes Evolution In Poeciliid Fishes, Andrew I. Furness, Bart J.A. Pollux, Robert Meredith, Mark S. Springer, David N. Reznick

Department of Biology Faculty Scholarship and Creative Works

In live-bearing animal lineages, the evolution of the placenta is predicted to create an arena for genomic conflict during pregnancy, drive patterns of male sexual selection, and increase the rate of speciation. Here we test these predictions of the viviparity driven conflict hypothesis (VDCH) in live-bearing poecilid fishes, a group showing multiple independent origins of placentation and extreme variation in male sexually selected traits. As predicted, male sexually selected traits are only gained in lineages that lack placentas; while there is little or no influence of male traits on the evolution of placentas. Both results are consistent with the mode …

Type Three Diabetes: Are You At Risk? A Systematic Review, Mary Ellen Yep

All Student-Created Educational Resources

To develop a possible framework for informing patients with type two diabetes of their potential risk of dementia, specifically Alzheimer’s Disease. Since type two diabetes is still on the rise, this could implicate a major increase in dementia across the population. Implications to assess the public of this research to promote awareness of the risks of type 3 diabetes.