Life Sciences Commons^™

Scaphanocephalus-Associated Dermatitis As The Basis For Black Spot Disease In Acanthuridae Of St. Kitts, West Indies, Michelle M. Dennis, Adrien Izquierdo, Anne Conan, Kelsey Johnson, Solenne Giardi, Paul Frye, Mark A. Freeman

Harold W. Manter Laboratory of Parasitology: Library Materials

Acanthurus spp. of St. Kitts and other Caribbean islands, including ocean surgeonfish A. bahianus, doctorfish A. chirurgus, and blue tang A. coeruleus, frequently show multifocal cutaneous pigmentation. Initial reports from the Leeward Antilles raised suspicion of a parasitic etiology. The aim of this study was to quantify the prevalence of the disease in St. Kitts’ Acanthuridae and describe its pathology and etiology. Visual surveys demonstrated consistently high adjusted mean prevalence at 3 shallow reefs in St. Kitts in 2017 (38.9%, 95% CI: 33.8−43.9) and 2018 (51.5%; 95% CI: 46.2−56.9). There were no differences in …

Novel Information On The Morphology, Phylogeny And Distribution Of Camallanid Mematodes From Marine And Freshwater Hosts In South Africa, Including The Description Of Camallanus Sodwanaensis N. Sp., Roman Svitin, Marliese Truter, Olena Kudlai, Nico J. Smit, Louis Du Preez

Harold W. Manter Laboratory of Parasitology: Library Materials

Four species of previously known nematodes from the family Camallanidae were found from different hosts in South Africa: Batrachocamallanus xenopodis from the frog Xenopus muelleri, Paracamallanus cyathopharynx, and Procamallanus pseudolaeviconchus from the catfish Clarias gariepinus and Spirocamallanus daleneae from the catfish Synodontis zambezensis. In the material collected from various marine fishes, several specimens of nematodes from the genus Camallanus clearly differed from all previously known species. Based on morphological differences these specimens are assigned to a new species, C. sodwanaensis. Molecular data of 18S and 28S rDNA and COI sequences are provided for the collected species …

Conceptualizing Ecological Responses To Dam Removal: If You Remove It, What’S To Come?, J. Ryan Bellmore, G. R. Press, Jeffrey J. Duda, Jim E. O'Connor, Amy E. East, Melissa M. Foley, Andrew C. Wilcox, Jon J. Major, Patrick Shafroth, Sarah A. Morley, Christopher S. Magirl, Chauncey W. Anderson, James E. Evans, Laura Shawn Craig

School of Earth, Environment and Society Faculty Publications

One of the desired outcomes of dam decommissioning and removal is the recovery of aquatic and riparian ecosystems. To investigate this common objective, we synthesized information from empirical studies and ecological theory into conceptual models that depict key physical and biological links driving ecological responses to removing dams. We define models for three distinct spatial domains: upstream of the former reservoir, within the reservoir, and downstream of the removed dam. Emerging from these models are response trajectories that clarify potential pathways of ecological transitions in each domain. We illustrate that the responses are controlled by multiple causal pathways and feedback …

External Marking And Behavior Of Early Instar Helicoverpa Armigera (Lepidoptera: Noctuidae) On Soybean, Luiz E. R. Pannuti, Edson L. L. Baldin, Silvana L. Vieira De Paula Moraes, Thomas E. Hunt, Vinicius F. Canassa, José P. F. Bentivenha, Ivana F. Da Silva

Department of Entomology: Faculty Publications

Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is a pest of major agricultural crops, such as soybean and cotton. A better understanding of larval movement is important for its integrated management and resistance management. Studies with neonates through second instar larvae are still limited by the difficulties involving the handling and observation of these instars. Many studies require marking larvae, and most research involving marking is focused on moths. However, our study investigated aspects of larval behavior of the second instar of H. armigera on soybean plants. The dyes luminous powder red and Sudan Red 7B were tested as external larval …

Spatial Repellency, Antifeedant Activity And Toxicity Of Three Medium Chain Fatty Acids And Their Methyl Esters Of Coconut Fatty Acid Against Stable Flies, Gwang H. Roh, Xiaaojie Zhou, Yansu Wang, Steven C. Cermak, James A. Kenar, Alexander Lehmann, Baoyu Han, David B. Taylor, Xiaopeng Zeng, Chung Gyoo Park, Gary J. Brewer, J.J. Zhu

Department of Entomology: Faculty Publications

BACKGROUND: Stable flies are one of the most detrimental arthropod pests to livestock. With changing climates and agronomic practices, they expand their roles as pests and disease vectors as well. Their painful bites reduce livestock productivity, annoy companion animals, and interfere with human recreational activities. Current management technologies are unable to effectively control stable flies. The present study reports new results concerning the contact, spatial repellency, and toxicity of a bio-based product, coconut fatty acid and their methyl ester derivatives of free fatty acids of C8:0, C10:0 and C12:0 to stable flies.

RESULTS: Three medium chain fatty acid methyl esters …

Histone Acetylation Increases In Response To Ferulic, Gallic, And Sinapic Acids Acting Synergistically In Vitro To Inhibit Candida Albicans Yeast‐To‐Hyphae Transition, Cristiane R. S. Câmara, Qin-Yin Shi, Matthew Pedersen, Richard Zbasnik, Kenneth Nickerson, Vicki Schlegel

Department of Food Science and Technology: Faculty Publications

Novel treatments are needed to prevent candidiasis/candidemia infection due to the emergence of Candida species resistant to current antifungals. Considering the yeast-to‐hyphae switch is a critical factor to Candida albicans virulence, phenols common in plant sources have been reported to demonstrating their ability to prevent dimorphism. Therefore, phenols present in many agricultural waste stress (ferulic (FA) and gallic (GA) acid) were initially screened in isolation for their yeast‐to‐hyphae inhibitory properties at times 3, 6, and 24 hr. Both FA and GA inhibited 50% of hyphae formation inhibitory concentration (IC₅₀) but at a concentration of 8.0 ± 0.09 and …

Effect Of Rootstocks On Phytochemical Properties Of Apricot Fruit, Müttali̇p Gündoğdu

Turkish Journal of Agriculture and Forestry

The phytochemical influence of rootstocks on the Hacıhaliloğlu apricot cultivar was examined in this study. Myrobolan 29-C, Myrobolan GF-31, Marianna 2624, Pixy, and Tokaloğlu rootstocks were used and organic acid, sugars, vitamin C, total antioxidant capacity, and phenolic compounds were identified. The highest phenolic compounds and antioxidant capacity were observed in Tokaloğlu rootstock. While Myrobolan GF-31 stood out in terms of organic acids and vitamin C, Pixy stood out in terms of sugar. The highest sugar content (sucrose) was recorded as 24.178 mg 100 g-1 in 'Pixy'. It was determined that Tokaloğlu rootstock had increased organic acid (1030.730 mg 100 …

Sap Flux And Stem Radius Variations In Mature Cedrus Libani Trees During The Growing Season, Ayli̇n Güney, Rei̇ner Zi̇mmermann, Klaus Haas

Turkish Journal of Agriculture and Forestry

Analysis of continuous sap flux and stem growth measurements can help to elucidate how environmental conditions influence water status and stem growth in trees. Stem radius variations and sap flux densities of the drought-tolerant conifer Cedrus libani were measured using automatic point dendrometers and heat dissipation sensors, respectively. The study was conducted at the Elmalı Cedar Research Forest at 1665 m a.s.l. From April to September 2009, stem radius variation was measured hourly for nine C. libani trees. Concurrently, environmental variables (e.g., air temperature, precipitation, relative humidity, soil water content) were monitored within the study site. From the dendrometer records, …

Tree Vigor, Yield, Fruit Quality, And Antioxidant Capacity Of Apple (Malus × Domestica Borkh.) Influenced By Different Fertilization Regimes: Preliminary Results, Tomo Milosevic, Nebojsa Milosevic, Jelena Mladenovic

Turkish Journal of Agriculture and Forestry

Tree growth, productivity, fruit quality attributes, and antioxidant activity were tested in apple cultivar Idared under different fertilization treatments from the 22nd to the 23rd year after planting in a heavy acidic soil under western Serbian conditions. Treatments included the use of aged cattle manure, compound NPK (15:15:15) mineral fertilizer, a multinutrient fertilizer commercially named Italpolina (ITP) (NPK + organic carbon + humic acids), two types of N mineral fertilizers (calcium ammonium nitrate [CAN] and urea), and control (no fertilization). Results showed that Idared trees were more vigorous when fertilized with the two N fertilizers than when treated with another …

Comparative Transcriptome Sequencing To Determine Genes Related To The Nucellar Embryony Mechanism In Citrus, Özhan Şi̇mşek, Di̇cle Dönmez, Si̇nan Eti̇, Turgut Yeşi̇loğlu, Yildiz Kaçar

Turkish Journal of Agriculture and Forestry

Several citrus varieties produce apomictic seeds by the nucellar embryony (NE) mechanism. Nucellar embryos are created from nucellus tissue and have an identical genetic constitution to the mother plant. Nucellar embryony is known to be an unusual feature of seed production in many citrus cultivars. The term "NE" refers to the development of identical embryos from the maternal tissue known as the nucellus surrounding the embryo sac. The authors aimed here to detect differentially expressed genes involved in the NE mechanism. Orlando tangelo (OT), producing apomictic seeds, and a clementine mandarin, Algerian tangerine ranch selection (AT), known as monoembryonic, were …

Characterization Of Quince (Cydonia Oblonga Mill.) Accessions By Simple Sequence Repeat Markers, Murat Güney, Sali̇h Kafkas, Aysen Koç, Servet Aras, Hakan Keles, Harun Karci

Turkish Journal of Agriculture and Forestry

In Turkey, quince (Cydonia oblonga Mill.) is found as both a wild and a cultivated tree species. The Karanlıkdere Valley is a pit area between Şefaatli and Yerköy districts in Yozgat Province that originates from the River Delice. Members of the Yozgat Karanlıkdere Valley quince population, containing 17 quince accessions and 15 commercial quince cultivars, were DNA fingerprinted using 30 simple sequence repeat (SSR) primers to identify the genetic relationships among them. A total of 111 alleles were detected for all 32 accessions, and the number of alleles revealed by SSR analysis ranged from 2 to 10 alleles per locus …

Effect Of Rhizobacteria Treatments On Nutrient Content And Organic And Amino Acid Composition In Raspberry Plants, Muzaffer İpek

Turkish Journal of Agriculture and Forestry

Plant growth-promoting rhizobacteria (PGPR) have been found to be beneficial to plant growth, yield, crop quality, the environment, and sustainable agricultural production. Therefore, six bacterial strains were tested to determine their effects on raspberry's nutrient content and organic and amino acid composition. The experiment was performed from 2015 to 2017. Two-year-old raspberry plants were inoculated with bacterial suspensions by a dipping method and were planted in 30-L pots. The mineral content and organic acid and amino acid composition of the leaf and root were compared in the Alcaligenes 637Ca, Staphylococcus MFDCa1 and MFDCa2, Agrobacterium A18, Pantoea FF1, and Bacillus M3 …

Comparison Of Maize Lines And Their Test Crosses According To Grain Yield And Somephysiological Properties, Eli̇f Özdemi̇r, Bayram Sade

Turkish Journal of Agriculture and Forestry

This study evaluated 7 inbred lines of maize (Zea mays L.), 3 testers, and 21 hybrids produced using the line × tester mating design. The study was conducted in Konya, in The Central Anatolia Region of Turkey. CD (coefficient of determination) values were calculated to detect the effect of inbred lines on grain yield and other physiological properties of the test crosses. The mean values of all the parameters of the genotypes were evaluated using the Duncan?s multiple range test for grouping progenies and parent groups. The CD values between parent groups and progenies were statistically significant for photosynthetic efficiency …

Environmentally Friendly Fertilizers Can Enhance Yield And Bioactive Compounds Inchinese Cabbage (Brassica Rapa Ssp. Pekinensis), Jeongyeo Lee, Young-Hee Noh, Kun-Hyang Park, Dae-Soo Kim, Han Taek Jeong, Haeng-Soon Lee, Sung Ran Min, Hyeran Kim

Turkish Journal of Agriculture and Forestry

The objective of this study was to evaluate the performance of 3 environmentally friendly fertilizers and their effect on the growth of Chinese cabbage and its contents of bioactive compounds. The following fertilizers were applied: purslane extract, 5% sulfur, and 15% phosphoric acid with 5% calcium. A total of 9, 9, and 11 components were detected for fatty acids, carotenoids, and glucosinolates, respectively. The contents of cis-7 hexadecenoic acid, linoleic acid (C18:2n6c+t), and linolenic acid (C183n3) were generally increased, and also carotenoid amounts of violaxanthin, antheraxanthin, lutein, 13-cis-ß-carotene, α-carotene, ß-carotene, and 9-cis-ß-carotene were significantly increased by fertilizer treatments. All fertilization …

Design And Development Of A Front Mounted On-The-Go Soil Strength Profile Sensor, Ari̇f Behi̇ç Teki̇n, Harun Yalçin

Turkish Journal of Agriculture and Forestry

Soil compaction is a great problem since it affects crop growth and yield. The causes of soil compaction are the management practices in agricultural production. A common practice is to implement subsoiling at a few centimeters below the hardpan. Management practices, field traffic, and variations of the soil?s physical and chemical properties throughout the field cause variations in the soil compaction degree and depth. Subsoiling at certain depths can cause excessive energy consumption at a high cost. Therefore, agricultural tillage equipment could be improved by varying the tillage depth. Soil strength is the main indicator that depends on several soil …

Design, Development, And Evaluation Of A Target Oriented Weed Control System Usingmachine Vision, Ömer Bariş Özlüoymak, Ali̇ Bolat, Ali̇ Bayat, Emi̇n Güzel

Turkish Journal of Agriculture and Forestry

The objective of the study was to develop and test an automatic machine vision-based spraying robot for the detection, tracking, and spraying of artificial weeds by using LabVIEW programming language. The greenness method was used to distinguish green objects in the image. A time-controlled spray nozzle was run according to the presence of an artificial weed and its coordinates. A mobile test bench was built and the spraying system with a webcam was operated at speeds of 0.42, 0.54, 0.66, 0.78, and 0.90 km h-1, so as to be able to see the performance of the system. The amount of …

Highly Polymorphic Novel Simple Sequence Repeat Markers From Class I Repeats Inwalnut (Juglans Regia L.), Emre Eser, Hayat Topçu, Sina Kefayati, Mehmet Sütyemez, Md Rashedul Islam, Sali̇h Kafkas

Turkish Journal of Agriculture and Forestry

Walnut (Juglans regia L.) is the most important species in the genus Juglans due to the high commercial value of its nuts and timber. Simple sequence repeats (SSRs) are considered as the markers of choice owing to their codominant nature. Since very few SSRs have been developed in walnut, this study aimed to develop numerous polymorphic SSRs from Class I repeats by using DNA sequences of the Chandler cultivar. In all, 800 SSRs were designed and tested in 8 Turkish, 3 French, and 5 US walnut cultivars, of which 88 (11%) did not produce bands, 161 (20.1%) were monomorphic, and …

Role Of Endogenous Polyamines In The Alternate Bearing Phenomenon In Pistachio, Muhammet Ali̇ Gündeşli̇, Sali̇h Kafkas, Mouzghan Zarifikhosroshahi, Nesi̇be Ebru Kafkas

Turkish Journal of Agriculture and Forestry

Alternate bearing is a significant economic problem adversely affecting the producers, consumers, and economy of a country. In the present study, we aimed to investigate the physiological role of free polyamines (PAs) in fruit bud abscission of the Uzun pistachio variety, which has a strong alternate bearing tendency. The identification and quantification of PAs, such as putrescine (Put), spermidine (Spd), and spermine (Spm), at different physiological stages and in different organs, were carried out by high-performance liquid chromatography. Putrescine was the major PA in all the organs of pistachio trees. Furthermore, the concentrations of Put and Spd in the leaves …

Mesophyll Protoplast Isolation Technique And Flow Cytometry Analysis Of Ancient Platycladus Orientalis (Cupressaceae), Qianyi Zhou, Zhaohong Jiang, Yiming Li, Tian Zhang, Hailan Zhu, Fei Zhao, Zhong Zhao

Turkish Journal of Agriculture and Forestry

Platycladus orientalis (Cupressaceae) is a native tree species used widely for landscaping and afforestation in arid and semiarid areas in Asia. Ancient P. orientalis trees not only have an important humanistic and historical value but also have a significant scientific value in aging mechanism research. Mesophyll protoplasts are an important material for studies of plant regeneration, transient gene expression, and senescence. Although an easy and effective technique of mesophyll protoplast isolation from mature trees is urgently needed, the isolation parameters have great material specificity. The young tissue of plants is an ideal material for protoplast preparation. In this study, we …

Authentication Of Gemlik Olive Cultivar Using 1h Nmr Spectroscopy Andchemometric Analysis, İbrahi̇m Sani̇ Özdemi̇r, Somer Beki̇roğlu

Turkish Journal of Agriculture and Forestry

In this study, 1H NMR spectroscopy was used to discriminate Gemlik olives cultivated in a protected designation of origin (PDO) region from those cultivated in non-PDO regions. For this purpose, 95 Gemlik olive samples (47 from PDO and 48 from non- PDO regions) were collected. 1H NMR spectral analysis was carried out on the oils extracted from these olive samples. The results of the partial least squares-discriminant analysis showed that olives from the PDO and non-PDO regions could be discriminated on the basis of their NMR profiles. The prediction ability of the model was better when olive samples from the …

Identification Of Chromosomal Regions In The Genetic Control Of Quality Traits In Durum Wheat (Triticum Turgidum L.) From The Fertile Crescent, Ahmad Alsaleh, Faheem Shahzad Baloch, Mohamad Azrak, Aladdin Hamwieh, Gönül Cömertpay, Rüştü Hati̇poğlu, Miloudi Nachit, Hakan Özkan

Turkish Journal of Agriculture and Forestry

Durum wheat genetic resources from Turkey and Syria are expected to harbor novel alleles for most traits that are of interest to breeders and consumers. However, there have not been sufficient efforts to investigate the genetic structure of this gene pool. In this study, cultivar Kunduru-1149 (selected from one of the unique landraces, Kunduru) from Anatolia was crossed with a Syrian cultivar, Cham1, to produce recombinant inbred lines (RILs) for quantitative trait locus (QTL) analysis. The RIL population was genotyped with simple sequence repeats, amplified fragment length polymorphism, and seed storage protein markers and analyzed for 9 different important quality …

Nematode-Resistant, Clonal Almond Rootstock Breeding By Crossing In Turkey, Hali̇t Seyfetti̇n Atli, Canan Can, Mehmet Baş, Kami̇l Sarpkaya, Ayşe Fi̇danci, Ertuğrul İli̇kçi̇oğlu, Nergi̇z Çoban, Sultan Bay Türkoğlu

Turkish Journal of Agriculture and Forestry

Nematode-resistant clonal rootstock use is limited in Turkey. Throughout a 3-year-study (2012-2014), targeted rootstock candidates were obtained through hybridization between almond and plum cultivars. Some characteristics, such as strong growth ability, compatibility with other almond cultivars, nematode resistance, and rooting of the cuttings were common among the hybrid types. In the hybridization studies, one almond cultivar (Ferragnes) and 2 plum (Myrobalan and Pissardi nigra, both belonging to Prunus cerasifera) were used as female parents, and 2 plum (Myrobalan and Pissardi nigra) and 2 almond cultivars [Ferragnes and AB3 (Amygdalus orientalis Mill. Type)] were used as male parents. In total, 6 …

Identification And Validation Of Microsatellite Markers In Strawberry Tree(Arbutus Unedo L.), Pedro Fazenda, Ricardo Pereira, Maria Fonseca, Jorge Carlier, José Leitão

Turkish Journal of Agriculture and Forestry

Strawberry tree (Arbutus unedo L.), an evergreen shrub/small tree of the family Ericaceae, is a main constituent of the Mediterranean basin flora; although it is also found in southwestern France, Macaronesia, and Ireland. The small fruits are edible but mostly used for preparation of preserves and jams, and for liquors such as the Portuguese traditional "aguardente de medronho". Traditionally cultivated by small farmers, often in consociation with Quercus sp., strawberry tree is presently emerging as a new important fruit crop cultivated in large orchards by modern export-oriented enterprises. This change of paradigm requires a growing role of plant breeding, upstream …

A Comparative Analysis Of The Volatile Components Of Green Tea Produced From Various Tea Cultivars In China, Lin-Long Ma, Dan Cao, Yan-Li Liu, Zi-Ming Gong, Pan-Pan Liu, Xiao-Fang Jin

Turkish Journal of Agriculture and Forestry

China is a major producer and consumer of green tea and possesses numerous high-quality tea cultivars, which can be used to make green tea. However, the green teas produced from these tea cultivars vary markedly in aroma. In this study, a sensory evaluation method and headspace solid-phase microextraction combined with GC-MS were employed to determine the primary substances that contribute to the aromas and key differences in the aroma components of green tea produced from 20 tea cultivars. The results revealed 57 major volatile components, among which aldehydes, alcohols, and esters were the most prevalent. For the volatile components, the …

Molecular Characterization Of Apple (Malus × Domestica Borkh.) Genotypes Originatingfrom Three Complementary Conservation Strategies, Anca Butiuc, Ana Coste, Anca Farkas, Victoria Cristea, Valentina Isac, Adela Halmagyi

Turkish Journal of Agriculture and Forestry

Apple (Malus × domestica Borkh.) genotypes originating from different plant collections (field collection, in vitro plant collections undergoing or not undergoing cryopreservation) were screened and characterized by SSR markers. Shoot tips excised from plants grown in vitro were successfully cryopreserved by encapsulation-dehydration. The highest regrowth frequency (69%, cultivar Goldrush) of cryopreserved apices was achieved after 24 h of osmoprotection in 0.5 M sucrose, 3 h of desiccation, and 24% water content of alginate beads. No differences in morphological characteristics including shoot length and number and length of roots were observed between controls and plants recovered after cryopreservation. SSR markers were …

The Effects Of Mistletoe (Viscum Album L.) On The Physiological Properties Of Some Drupetrees In Turkey, Tamer Üstüner

Turkish Journal of Agriculture and Forestry

In the present study, the effects of mistletoe (Viscum album L.) on the physiological properties of drupe trees were investigated. In order to achieve this goal, sulfhydryl, chlorophyll a and b, total chlorophyll, ascorbic acid, total amino acid, proline, carotenoid, glucose, and fructose levels, as well as leaf proportional water contents were determined in both mistletoe and its hosts under laboratory conditions. According to the results, mistletoe caused a significant decrease in water content and reduced total ascorbic acid content in apricot, almond, and plum. Additionally, mistletoe did not affect the total amino acid, glucose, and fructose concentrations of the …

Effects Of Depth-Dependent Irrigation Regimes And Organomineral Fertilizers On Water Useand Quality Attributes Of Sugar Beet, Ali̇ Ünlükara

Turkish Journal of Agriculture and Forestry

Experiments were conducted under provincial conditions of Kayseri, Turkey, for two years in the growing seasons of 2014 and 2015 to determine the effects of irrigation water applications based on different sugar beet root zone depths and different organomineral fertilizers on sugar beet yield, root quality, water consumption, and water use efficiency. Two constant root depths (D1 = 0.9 m and D2 = 0.6 m) and one active water extraction root depth (D3= 0.4-0.9 m) were investigated. A mineral fertilizer (F1), an organomineral fertilizer (F2), and organic + mineral fertilizer (F3) were considered as the subtreatments in the experiments carried …

Using Smoke Solutions In Grass Pea (Lathyrus Sativus L.) To Improve Germination And Seedling Growth And Reduce Toxic Compound Odap, Uğur Başaran, Medi̇ne Çopur Doğrusöz, Erdem Gülümser, Hani̇fe Mut

Turkish Journal of Agriculture and Forestry

The effects of smoke solutions derived from wheat straw on the germination, seedling growth characteristics, and biochemical content of grass pea (Lathyrus sativus L.) were examined in this study. The smoke solutions were applied to petri and pot media at different concentrations. Both experiments were conducted in a randomized plot design with 3 replications. In the pot experiments, the smoke solutions were applied through the initial irrigation at 5 different concentrations (1%, 5%, 10%, 20%, and 40%) while 6 concentrations were used for petri experiments (0.5%, 1%, 2%, 4%, 8%, 10%) and results were compared to a control (distilled water). …

Identification And Characterization Of Hypovirus-Infected Cryphonectria Parasitica Isolates From Biological Control Plots In İzmir, Kütahya, And Sinop, Seçi̇l Akilli Şi̇mşek, Yakup Zekai̇ Katircioğlu, Nora Borst, Deni̇z Çakar, Simone Prospero, Daniel Rigling, Sali̇h Maden

Turkish Journal of Agriculture and Forestry

In a pilot study, a biological control with hypovirus-infected Cryphonectria parasitica was applied in 3 study plots in Turkey, in June 2013. The aim of this study was to evaluate the success of the applied biological control by verifying the establishment of the released hypovirus (CHV-1) strains using molecular markers. C. parasitica isolates were sampled from cankers at 3 different time points: before the biological control treatments (April 2013), and 5 months (October 2013) and 11 months (May 2014) after the treatment. In total, 255 C. parasitica isolates were recovered and characterized. First, the culture morphology and vegetative compatibility type …

Development Of An In Vitro Micropropagation Protocol For Myrobalan 29c Rootstock, Murat Güney

Turkish Journal of Agriculture and Forestry

Stone fruits are well known for their high nutritional value. Therefore, in horticulture, the micropropagation of suitable rootstocks is vital for their cultivation. The aim of the present study was to improve the micropropagation protocol of Myrobalan 29C (Prunus cerasifera Ehrh.) rootstock using shoot-tip culture. Murashige and Skoog (MS) basal medium containing 2 mg L-1 6-benzylaminopurine (BAP) and 0.05 mg L-1 gibberellic acid (GA3) resulted in the highest number (14.3) of multiple shoots. However, a greater shoot length (2.0 cm) was attained when GA3 was excluded from the MS medium and the concentration of BAP was reduced to 1 mg …