Life Sciences Commons^™

Seed Dormancy-Breaking In A Cold Desert Shrub In Relation To Sand Temperature And Moisture, Huiliang Liu, Buhailiqiemu Abudureheman, Lingwei Zhang, Jerry M. Baskin, Carol C. Baskin, Daoyuan Zhang

Biology Faculty Publications

Seasonal periodicity of seed germination and its relationship to seasonal changes in temperature and soil moisture have been well studied in seeds of species with physiological dormancy. However, relatively little information is available on the role of these environmental factors in controlling germination of seeds with physical dormancy (PY). Our primary aim was to determine if seeds of the cold desert sand dune semi-shrub Eremosparton songoricum exhibits seasonal periodicity of seed germination and the relationship between seed dormancy break and soil temperature and moisture. In the laboratory, seeds incubated on dry, wet, wet-dry and dry-wet sand were exposed to a …

Microbial Interactions In The Phyllosphere Increase Plant Performance Under Herbivore Biotic Stress, Muhammad Saleem, Nicole Meckes, Zahida H. Pervaiz, Milton B. Traw

Plant and Soil Sciences Faculty Publications

The phyllosphere supports a tremendous diversity of microbes and other organisms. However, little is known about the colonization and survival of pathogenic and beneficial bacteria alone or together in the phyllosphere across the whole plant life-cycle under herbivory, which hinders our ability to understand the role of phyllosphere bacteria on plant performance. We addressed these questions in experiments using four genetically and biogeographically diverse accessions of Arabidopsis thaliana, three ecologically important bacterial strains (Pseudomonas syringae DC3000, Xanthomonas campestris, both pathogens, and Bacillus cereus, plant beneficial) under common garden conditions that included fungus gnats (Bradysia spp.). …

Moderator Biographies, Heart Of America Grazing Conference

Kentucky Grazing Conference

No abstract provided.

Grazing Panel, Jeff Beasley, Trevor Toland, Ted Krauskopf

Kentucky Grazing Conference

No abstract provided.

Cover Crops/Annuals And Grazing, Roger Staff

Kentucky Grazing Conference

The use of annual forages (cover crops), both summer and winter, have been utilized for several years. When cover crops first were used to extend the grazing season, it began with one to three species in a mixture. That was before the soil health benefits were being observed and discussed as they are now. There have been a wide variety of planting methods used: airplanes, helicopters, conventional and No-till drills. Some seeding methods is simply broadcast on the soils surface and lightly tilled in or just rained in depending upon the weather. Then we discovered the herbicides and amounts used …

Mitigating The Challenges Of Grazing Lush, Spring Forages, W. Travis Meteer

Kentucky Grazing Conference

During the winter season most cattle are supplemented with dry forages, grains, and co-products. This ration is balanced and delivered to cattle. Then spring comes along and cattle are put out to grass. While green grass solves a lot of problems associated with winter feeding (manure, pen maintenance, calf health, and labor demands), it can pose nutritional challenges. Lush, spring forage has three major challenges when it comes to meeting cattle nutrition requirements.

Seedhead Suppression In Tall Fescue With Chaparral Herbicide, E. S. Flynn, P. B. Burch

Kentucky Grazing Conference

Tall Fescue (Lolium arundinaceum (Schreb.)) is cool-season perennial grass found in pastures throughout the Eastern US, most commonly in the transition zone. It’s tolerance to extreme temperatures, drought, poor soil fertility, heavy grazing, and pests have made it a reliable forage base for livestock pastures. Released in 1943, ‘KY-31’ tall fescue was quickly adopted by cattle producers and now inhabits an estimated 40 million acres in the US.

Soon after its adoption, cattle producers began to notice a reduction in animal performance and signs that animals were heat stressed following tall fescue consumption. These symptoms were an indication of an …

Fescue Tolerance Testing, Monty Kerley

Kentucky Grazing Conference

Fescue toxicosis is characterized by clinical symptoms such as vasoconstriction, immunosuppression, and poor thermoregulation. Fescue toxins bind to membrane receptors of cells that control constriction of blood through capillaries responsible for heat dissipation. When cattle consume toxic tall fescue, they lose ability to move blood to their skin where heat can be lost to the environment. As they lose this ability they become more prone to heat stress. In the winter, this lack of blood flow leads to other clinical symptoms, such as fescue foot and loss of tail switch, that occur from restricted blood flow.

Research Update: Beef Cattle Management Considerations For Grazing Tall Fescue, Frank Ireland

Kentucky Grazing Conference

It has been reported that 8 million beef cattle graze an estimated 35 million acres of endophyte-infected tall fescue in the United States with the majority of tall fescue being produced in the Midwest and southeastern regions of the United States. In the presence of the endophytic fungus, ergot alkaloids are present that when consumed by beef cattle results in decreased animal performance as measured in reduced weight gains, lower body condition scores, decreased conception rates, rough hair coats and the inability to regulate body temperatures. It has been estimated that this loss in animal performance accounts for a $600 …

In Defense Of Soil Health, Allen R. Williams

Kentucky Grazing Conference

Soil health is a hot topic today in sustainable agriculture circles and is even becoming more popular in conventional agriculture. However, the term is often thrown around loosely, and sometimes the meaning gets muddled.

Foreword And Conference Information [2017], Matt Bunger

Kentucky Grazing Conference

This is the front matter of the proceedings.

Organelle_Pba, A Pipeline For Assembling Chloroplast And Mitochondrial Genomes From Pacbio Dna Sequencing Data, Aboozar Soorni, David Haak, David Zaitlin, Aureliano Bombarely

Kentucky Tobacco Research and Development Center Faculty Publications

Background: The development of long-read sequencing technologies, such as single-molecule real-time (SMRT) sequencing by PacBio, has produced a revolution in the sequencing of small genomes. Sequencing organelle genomes using PacBio long-read data is a cost effective, straightforward approach. Nevertheless, the availability of simple-to-use software to perform the assembly from raw reads is limited at present.

Results: We present Organelle-PBA, a Perl program designed specifically for the assembly of chloroplast and mitochondrial genomes. For chloroplast genomes, the program selects the chloroplast reads from a whole genome sequencing pool, maps the reads to a reference sequence from a closely related species, and …

Fourth-Generation Fan Assessment Numeration System (Fans) Design And Performance Specifications, Michael P. Sama, George B. Day, Laura M. Pepple, Richard S. Gates

Biosystems and Agricultural Engineering Faculty Publications

The Fan Assessment Numeration System (FANS) is a measurement device for generating ventilation fan performance curves. Three different-sized FANS currently exist for assessing ventilation fans commonly used in poultry and livestock housing systems. All FANS consist of an array of anemometers inside an aluminum shroud that traverse the inlet or outlet of a ventilation fan. The FANS design has been updated several times since its inception and is currently in its fourth-generation (G4). The current design iteration (FANS-G4) is reported in this article with an emphasis on the hardware and software control, data acquisition systems, and operational reliability. Six FANS-G4 …

Comparative Proteomic Analyses Of The Parietal Lobe From Rhesus Monkeys Fed A High-Fat/Sugar Diet With And Without Resveratrol Supplementation, Relative To A Healthy Diet: Insights Into The Roles Of Unhealthy Diets And Resveratrol On Function, Aaron M. Swomley, Judy C. Triplett, Jeriel T. Keeney, Govind Warrier, Kevin J. Pearson, Julie A. Mattison, Rafael De Cabo, Jian Cai, Jon B. Klein, D. Allan Butterfield

Chemistry Faculty Publications

A diet consisting of a high intake of saturated fat and refined sugars is characteristic of a Western-diet and has been shown to have a substantial negative effect on human health. Expression proteomics were used to investigate changes to the parietal lobe proteome of rhesus monkeys consuming either a high fat and sugar (HFS) diet, a HFS diet supplemented with resveratrol (HFS+RSV), or a healthy control diet for 2 years. Here we discuss the modifications in the levels of 12 specific proteins involved in various cellular systems including metabolism, neurotransmission, structural integrity, and general cellular signaling following a nutritional intervention. …

Effects Of Biochar Treatment Of Municipal Biosolids And Horse Manure On Quality Of Runoff From Fescue Plots, Rachel Williams, Dwayne R. Edwards

Biosystems and Agricultural Engineering Faculty Publications

Land-applied horse manure and municipal biosolids can increase nutrient and bacteria concentrations in runoff. Biochar has been demonstrated to have beneficial impacts on nutrient retention and runoff quality when used to treat other land-applied organic soil amendments (e.g., poultry manure). The objective of this study was to evaluate the effects of biochar addition to horse manure and municipal biosolids on runoff concentrations of nutrients and fecal coliforms. Biochar was added at 5% to 8% (wet basis) to horse manure and biosolids that were applied to 2.4 m x 6.1 m fescue plots followed by application of simulated rainfall (102 mm …

Synthesis And Development Of Zwitterionic Pei (Zpei) For Optimized Delivery Of Nucleic Acids, Joseph Raleigh Duke Iii

Theses and Dissertations--Chemistry

Gene therapy holds promise for the treatment a wide range of diseases ranging from cystic fibrosis to cardiovascular disease to cancer. The need for safe and efficient gene delivery methods remains the primary barrier to human gene therapy. Non-viral vector materials, including polymers, can be designed to be biocompatible and non-immunogenic, but lack the efficiency to be clinically relevant. Gene therapy awaits the development of new materials that are both safe and efficient. Here, we have synthesized a series of modified zwitterionic polymers based on the common transfecting agent polyethylenimine (PEI). Using a variety of biochemical and biophysical methods we …

Ammonia And Carbon Dioxide Emissions Of Three Laying-Hen Housing Systems As Affected By Manure Accumulation Time, Timothy A. Shepherd, Hongwei Xin, John P. Stinn, Morgan D. Hayes, Yang Zhao, Hong Li

Biosystems and Agricultural Engineering Faculty Publications

Laying-hen housing design and management are the most significant factors affecting the generation and release of gaseous ammonia to the atmosphere. Transitioning the hen housing type from traditional high-rise (where manure is stored within the house for about one year) to modern manure-belt style (where manure is removed every 1 to 4 d and placed into long-term storage) has significantly improved in-barn air quality and reduced farm-level ammonia emissions. As a direct result of the advantages, 100% of new construction for U.S. egg production incorporates manure-belt systems that regularly remove manure from the houses. However, manure-belt system designs (e.g., active …

Fluvial-Lacustrine Processes Shaping The Landforms Of The Distal Paraguay Fluvial Megafan, Edward Limin Lo

Theses and Dissertations--Earth and Environmental Sciences

Tropical wetlands such as the Pantanal help regulate global biogeochemical cycles, but climate change is modifying these environments. Controls on environmental changes can potentially be assessed from ancient, well-dated lacustrine sedimentary records. An integrated field and laboratory approach was undertaken to study the limnogeology of Lake Uberaba in the northern Pantanal, and test whether the lake has preserved a reliable record of environmental change in its strata. This study was designed to understand how the basin accumulates sediment and to assess its sensitivity to hydroclimatic variability. The data showed that modern Lake Uberaba is a highly dynamic, freshwater fluvial-lacustrine basin. …

A Method For Reflectance Index Wavelength Selection From Moisture-Controlled Soil And Crop Residue Samples, Ali Hamidisepehr, Michael P. Sama, Aaron P. Turner, Ole O. Wendroth

Biosystems and Agricultural Engineering Faculty Publications

Reflectance indices are a method for reducing the dimensionality of spectral measurements used to quantify material properties. Choosing the optimal wavelengths for developing an index based on a given material and property of interest is made difficult by the large number of wavelengths typically available to choose from and the lack of homogeneity when remotely sensing agricultural materials. This study aimed to determine the feasibility of using a low-cost method for sensing the moisture content of background materials in traditional crop remote sensing. Moisture-controlled soil and wheat stalk residue samples were measured at varying heights using a reflectance probe connected …

Kentucky Water Resources Research Institute Annual Technical Report Fy 2016, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2016 Annual Technical Report for Kentucky consolidates reporting requirements for the Section 104(b) base grant award into a single document that includes: 1) a synopsis of each student research enhancement project that was conducted during the period, 2) citations for related publications, reports, and presentations, 3) a description of information transfer activities, 4) a summary of student support during the period, and 5) notable awards and achievements.

No funds were requested for general program administration activities. However, travel funds were provided to support the participation of the director and associate director in the annual meeting of the National Institutes …

Statistical Analyses To Detect And Refine Genetic Associations With Neurodegenerative Diseases, Yuriko Katsumata

Theses and Dissertations--Epidemiology and Biostatistics

Dementia is a clinical state caused by neurodegeneration and characterized by a loss of function in cognitive domains and behavior. Alzheimer’s disease (AD) is the most common form of dementia. Although the amyloid β (Aβ) protein and hyperphosphorylated tau aggregates in the brain are considered to be the key pathological hallmarks of AD, the exact cause of AD is yet to be identified. In addition, clinical diagnoses of AD can be error prone. Many previous studies have compared the clinical diagnosis of AD against the gold standard of autopsy confirmation and shown substantial AD misdiagnosis Hippocampal sclerosis of aging (HS-Aging) …

Evaluating Soil Physical And Chemical Properties Following Addition Of Non-Composted Spent Coffee And Tea For Athletic Fields, Shuang Zhou

Theses and Dissertations--Plant and Soil Sciences

Physical and chemical properties of non-composted spent coffee (CF) and tea (T) suggest they may have applications as soil amendments for improving poor soils. Studies were conducted to determine 1) the effect of amendments on grass growth and soil properties, 2) the effect of incorporation versus surface application of amendments on soil properties, and 3) the effect of amendment application frequency on grass growth and soil properties. In the first study, amendments were mixed with sand and planted to bermudagrass. Treatments included CF, T, and peat moss (PM) mixed with sand, and 100% sand as a control. In the second …

Oxidative Stress And Proteomic Studies Of Mammalian Models Of Age-Related Metabolic Dysfunction In Neurodegenerative Disorders, Aaron M. Swomley

Theses and Dissertations--Chemistry

Expression proteomics is the field of science wherein proteins that make up the cellular proteome are identified both by name and by fold-change. Depending on the application of proteomics, this change in level could be due to internal cellular stressors or introduction of xenobiotics. Global oxidative stress measures use immunohistochemistry to determine the relative level of oxidative stress of macromolecules within the cell. In this dissertation, both global oxidative stress measures and expression proteomics were used in a variety of mammalian models in order to determine the effects of protein upregulation, intranasal insulin administration, and resveratrol supplementation on the cellular …