Life Sciences Commons^™

Denitrification In Urban Coastal Environments: A Functional Gene Study, Samantha Denise Lindemann

Master's Theses

Eutrophication is a major anthropogenic stressor on aquatic ecosystems worldwide. Jamaica Bay is located in the southeastern portion of New York City, NY (NYC). Like many other estuaries, Jamaica Bay is impacted by anthropogenic N inputs from various sources. Despite eutrophic conditions, improvements in water quality over the last few decades have prompted government agencies to promote oyster restoration to help reduce anthropogenic N in NYC waters. The effect of eastern oysters on sediment bacterial communities responsible for denitrification in Jamaica Bay was previously unknown. Those samples exposed to oysters were predicted to have high denitrification gene abundances, and thus …

Characterization Of The Female Urinary Microbiota And Their Association With The Female Bladder Uroepithelium, Evann Elizabeth Hilt

Master's Theses

The current clinical dogma assumes that urine is sterile in the absence of clinically relevant infection. However, recent evidence has demonstrated the existence of a female urinary microbiota in women with and without lower urinary tract symptoms. With the knowledge that the lower urinary tract possesses its own unique microbiota, I hypothesize that certain bacterial species of the female microbiota may be the cause or play a role in lower urinary tract syndromes such as overactive bladder syndrome (OAB). About 40-50% of OAB patients do not respond to conventional anti-muscarinic and beta-3 adrenergic agonist drug treatment. One possible explanation for …

An Investigation Of The Role Of Vibrio Vulnificus Rbda And Vibrio Parahaemolyticus Sypa In Biofilm Formation, Cecilia Thompson

Master's Theses

Vibrio vulnificus and Vibrio parahaemolyticus are both bacteria that cause human infection. V. vulnificus has a polysaccharide locus, rbd, responsible for bacterial aggregation, a form of biofilm. This locus is conserved in V. parahaemolyticus and in the non-pathogen V. fischeri.

In V. fischeri, the polysaccharide locus, syp, has been extensively characterized and shown to be important for biofilm formation. In V. fischeri, the first gene, sypA, is critical for biofilm formation. V. fischeri biofilm-proficient strains form wrinkled colonies. In contrast, sypA mutants form smooth colonies, indicating a lack of biofilm formation.

To understand the function of RbdA and SypAVP, proteins …

Identification And Analysis Of Omentum Derived Suppressor Cells In Regards To Th17 Inhibition, Nick Huang

Dissertations

Omentum has been harnessed by surgeons for hundreds of years, providing an ideal environment for graft healing and acceptance. However, little is known about the cellular mechanisms that promote the tolergenic environment of the omentum. We examined the cellular composition and role of activated omentum in regards to T-cell immunomodulation. We then tested activated omentum as a cellular therapy in a mouse allogenic lung transplantation model.

Our findings demonstrated activated omentum is mostly comprised of non-hematopoietic cells resembling mesenchymal stem-like cells (MSCs) and myeloid derived suppressor cells (MDSCs). Activated omentum exhibited anti-inflammatory properties through suppression of Th1 and Th17, while …

Multifunctional Coronavirus Papain-Like Proteases As Targets For Antiviral Therapeutics And Vaccines, Anna Maria Mielech

Dissertations

Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) and Middle Ease Respiratory Syndrome coronavirus (MERS-CoV) pose a severe threat to humans because of high mortality. Despite the risk of coronavirus (CoV) emerging in the human population there are no antiviral drugs or vaccines to combat coronavirus infection. The focus of my dissertation was to study the multifunctionality of papain-like proteases (PLPs) encoded within coronavirus genomes to facilitate the development of antiviral drugs and vaccines. The viral PLPs are critical for processing the amino-terminal end of the replicase during virus replication and are attractive targets for antiviral therapies.

In my research, I analyzed …

Generalist And Specialist Pollination Syndromes: When Are They Favoured? A Theoretical Approach To Predict The Conditions Under Which A Generalist Or Specialist Pollination Syndrome Is Favoured., Tyler L. Poppenwimer

Senior Independent Study Theses

No abstract provided.

What Does Not Kill Them Makes Them Stronger: Larval Environment And Infectious Dose Alter Mosquito Potential To Transmit Filarial Worms, Jennifer A. Breaux, Molly K. Schumacher, Steven A. Juliano

Faculty Publications – Biological Sciences

For organisms with complex life cycles, larval environments can modify adult phenotypes. For mosquitoes and other vectors, when physiological impacts of stressors acting on larvae carry over into the adult stage they may interact with infectious dose of a vector-borne pathogen, producing a range of phenotypes for vector potential. Investigation of impacts of a common source of stress, larval crowding and intraspecific competition, on adult vector interactions with pathogens may increase our understanding of the dynamics of pathogen transmission by mosquito vectors. Using Aedes aegypti and the nematode parasite Brugia pahangi, we demonstrate dose dependency of fitness effects of …

Estimating U.S. Household Seafood Demand Based On Longitudinal And Cross-Sectional Data, Huabo Wang

LSU Doctoral Dissertations

This overall goal of this research is to examine and update U.S. at-home household demand estimates for seafood (including species and generic products) that can be used in policy formulation (by both the private and public sector). As such, this study estimated quantity-based household demand functions for seafood in aggregate, by generic product form (fresh, frozen, and prepared), and primary species. Given that the role of quality has been shown to significantly influence expenditures and seafood demand, this paper also seeks to incorporate the household quality choice into the demand model. In order to help to tailor the market strategy, …

Production Of 2-Propanol, Butanol And Ethanol Using Clostridium Beijerinckii Optonii, Adam Hoogewind

LSU Doctoral Dissertations

With an unpredictable market for gasoline and increased concerns with the pollution created by burning fossil fuels, there is a push for developing suitable replacements for gasoline. While corn-based ethanol production is the most common renewable biofuel produced in the United States, ethanol is not an ideal solution to gasoline replacement due to low energy density, hygroscopic and corrosive properties and inability to purify by distillation alone. Higher alcohols such as butanol do not have the same problems with energy density, purification and hygroscopic and corrosive properties. The fermentation of butanol by using solventogenic Clostridium species, creating acetone, butanol and …

Occurrence Of Multiple Antibiotic Resistant Bacteria In Aquatic Environments In Central Minnesota, Megan Bollin

Honors Theses, 1963-2015

Antibiotic resistance among bacteria has been a topic of concern for many years. The purpose of this study was to evaluate the occurrence of antibiotic resistance among bacteria found in aquatic environments such as the lakes on the campus of Saint John’s University along with the Sauk River in Cold Spring and St. Joseph, Minnesota. By analyzing samples from different aquatic environments, this data can be used to better identify patterns of resistance within different genera of bacteria. A total of 125 isolates were captured from these different areas and isolated into pure cultures. The isolated cultures were grown on …

Fatigue Resistibility And Stimulus Strength Using Intraspinal Microstimulation Vs. Intramuscular Stimulation In A Rat Model: Case Study, John Dube

Honors Theses, 1963-2015

Spinal Cord Injury is a devastating injury that has drastic effects on an individual’s daily living activities. The current devices and methods of direct muscle, peripheral nerve cuff, and epidural stimulation are bulky, easily fatigable, require large stimulation parameters, and/ or difficult to use. Intraspinal Micro stimulation (ISMS) suggests a more fatigue resistant method at lower stimulation parameters that will help restore locomotion, bladder control, and sexual function in an individual. The research conducted herein shows ISMS evokes a linear positive relationship between stimulus strength and muscle contraction force that is comparable to clinically used methods of Intramuscular stimulation. Research …

Albedo-Induced Radiative Forcing From Mountain Pine Beetle Outbreaks In Forests, South-Central Rocky Mountains: Magnitude, Persistence, And Relation To Outbreak Severity, M. Vanderhoof, C. A. Williams, Y. Shuai, D. Jarvis, Dominik Kulakowski, J. Masek

Geography

Mountain pine beetle (MPB) outbreaks in North America are widespread and have potentially persistent impacts on forest albedo and associated radiative forcing. This study utilized multiple data sets, both current and historical, within lodgepole pine stands in the south-central Rocky Mountains to quantify the full radiative forcing impact of outbreak events for decades after outbreak (0-60 yr) and the role of outbreak severity in determining that impact. Change in annual albedo and radiative forcing peaked at 14-20 yr post-outbreak (0.06 ± 0.006 and-0.8 ± 0.1 W m-2, respectively) and recovered to pre-outbreak levels by 30-40 yr post-outbreak. Change in albedo …

Exchange Rate Volatility Effects On Brics Countries Exports, David Isaias Maradiaga Pineda

LSU Doctoral Dissertations

The leaders of the leading emerging economies Brazil, Russia, India, China, and South Africa (BRICS) claim that increased currency exchange rate volatility (CERV) from USD, EUR, and JPY (G-3) negatively impacts their exports, and expressed their desire for less trade dependence on these currencies. The literature on the impact of CERV on trade is vast. However, no consensus on the impact’s direction and significance has been reached yet. The motivation of this study was: first, to contribute to the existing empirical literature by using an alternative methodology; and second, to provide empirical evidence to the claim’s validity or nullity by …

The Short-Term Effects Of A Routine Poisoning Campaign On The Movements And Detectability Of A Social Top-Predator., B L. Allen, R. M. Engeman, L. K-P. Leung

United States Department of Agriculture Wildlife Services: Staff Publications

Top-predators can be important components of resilient ecosystems, but they are still controlled in many places to mitigate a variety of economic, environmental and/or social impacts. Lethal control is often achieved through the broadscale application of poisoned baits. Understanding the direct and indirect effects of such lethal control on subsequent movements and behaviour of survivors is an important pre-requisite for interpreting the efficacy and ecological outcomes of top predator control. In this study, we use GPS tracking collars to investigate the fine-scale and short-term movements of dingoes (Canis lupus dingo and other wild dogs) in response to a routine poison-baiting …

New Rodenticides: An Update On Recent Research Trials, Gary W. Witmer, Katherine E. Horak, Rachel Moulton

United States Department of Agriculture Wildlife Services: Staff Publications

Rodents cause substantial amounts of damage and losses of foodstuffs around the world. While various methods are used to reduce damage and losses to rodents, rodenticides remain the most important tool in the toolbox. However, like all tools, rodenticides have advantages and disadvantages. Several considerations are shaping the future of rodenticide use. These include manufacturing and registration costs, concern about toxicity levels and non-target animal hazards, potential hazards to children, reduced effectiveness of some formulations, and humaneness to the targeted rodents. While there have been very few new developments in rodenticides in the last several decades, new formulations and active …

Functional Significance Of Ultraviolet Feeding Cues In Wild Turkeys, Scott J. Werner, Richard Buchholz, Shelagh K. Tupper, Susan E. Pettit, Jeremy W. Ellis

United States Department of Agriculture Wildlife Services: Staff Publications

Most birds are able to sense ultraviolet (UV) visual signals. Ultraviolet wavelengths are used for plumage signaling and sexual selection among birds. The aim of our study was to determine if UV cues are also used for the process of food selection in wild turkeys (Meleagris gallopavo). We used avoidance conditioning to test the hypothesis that UV feeding cues can be used functionally for foraging behavior in wild turkeys. Female turkeys exhibited no avoidance of untreated food and 75–98% avoidance of food treated with an UV-absorbent, postingestive repellent (0.5–4% anthraquinone; wt./wt.) during repellent exposure. Male turkeys exhibited 78–99% avoidance of …

Wildlife Strikes With Military Rotary-Wing Aircraft During Flight Operations Within The United States, Brian E. Washburn, Paul J. Cisar, Travis L. Devault

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife–aircraft collisions (wildlife strikes) with civilian and military aircraft pose notable risks and economic losses. The 4 military services within the U.S. Department of Defense maintain records regarding wildlife strikes with military aircraft. Although rotary-wing aircraft operations comprise important mission components within all 4 military services, no assessment of wildlife strikes to military rotary-wing aircraft has been conducted. The objectives of this project were to 1) conduct a comprehensive analysis of data available from all military services regarding wildlife strikes with rotary-wing aircraft, and 2) provide recommendations to reduce the frequency and negative impacts of these strikes. We acquired all …

Animal Attendance At M-44 Sodium Cyanide Ejector Sites For Coyotes, John A. Shivik, Lauren Mastro, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Sodium cyanide (NaCN) ejectors for coyotes (Canis latrans), known as M-44s, are used in many parts of the United States for lethally removing coyotes to protect livestock or other resources. Quantifying selectivity of current devices in killing target and non-target species is important to users and provides a baseline for future development of more effective and selective techniques. We used motion-activated cameras to monitor M-44 locations for coyote and other species visitations to the sites. Because camera presence potentially influences coyote behavior, we first compared activation of M-44s at paired sites where cameras were and were not present. Coyotes activated …

Biology And Impacts Of Pacific Island Invasive Species. 11. Rattus Rattus, The Black Rat (Rodentia: Muridae), Aaron B. Shiels, William C. Pitt, Robert T. Sugihara, Gary W. Witmer

United States Department of Agriculture Wildlife Services: Staff Publications

The black rat, roof rat, or ship rat (Rattus rattus L.) is among the most widespread invasive vertebrates on islands and continents, and it is nearly ubiquitous on Pacific islands from the equatorial tropics to approximately 55 degrees latitude north and south. It survives well in human-dominated environments, natural areas, and islands where humans are not present. Rattus rattus is typically the most common invasive rodent in insular forests. Few vertebrates are more problematic to island biota and human livelihoods than R. rattus; it is well known to damage crops and stored foods, kill native species, and serve as a …

Toxicokinetics And Coagulopathy Threshold Of The Rodenticide Diphacinone In Eastern Screech-Owls (Megascops Asio), Barnett A. Rattner, Katherine E. Horak, Rebecca S. Lazarus, David A. Goldade, John J. Johnston

United States Department of Agriculture Wildlife Services: Staff Publications

In the United States, new regulations on second-generation anticoagulant rodenticides will likely be offset by expanded use of first-generation anticoagulant rodenticides. In the present study, eastern screech-owls (Megascops asio) were fed 10 mg diphacinone/g wet weight food for 7 d, and recovery was monitored over a 21-d postexposure period. By day 3 of exposure, diphacinone (DPN) was detected in liver (1.63 mg/g wet wt) and kidney (5.83 mg/g) and coagulopathy was apparent. By day 7, prothrombin time (PT) and Russell’s viper venom time (RVVT) were prolonged, and some individuals were anemic. Upon termination of exposure, coagulopathy and anemia were resolved …

Detecting An Elusive Invasive Species: A Diagnostic Pcr To Detect Burmese Python In Florida Waters And An Assessment Of Persistence Of Environmental Dna, Antoinette J. Piaggio, Richard M. Engeman, Matthew W. Hopken, John S. Humphrey, Kandy L. Keacher, William E. Bruce, Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

Recent studies have demonstrated that detection of environmental DNA (eDNA) from aquatic vertebrates in water bodies is possible. The Burmese python, Python bivittatus, is a semi-aquatic, invasive species in Florida where its elusive nature and cryptic coloration make its detection difficult. Our goal was to develop a diagnostic PCR to detect P. bivittatus from water-borne eDNA, which could assist managers in monitoring this invasive species. First, we used captive P. bivittatus to determine whether reptilian DNA could be isolated and amplified from water samples. We also evaluated the efficacy of two DNA isolation methods and two DNA extraction kits commonly …

A Landscape-Based Approach For Delineating Hotspots Of Wildlife-Vehicle Collisions, Nathan P. Snow, David M. Williams, William F. Porter

United States Department of Agriculture Wildlife Services: Staff Publications

Imposing human perceptions about the scales of ecological processes can produce unreliable scientific inferences in wildlife research and possibly misinform mitigation strategies. An example of this disconnect occurs in studies of wildlife-vehicle collisions (WVCs). Subjective procedures are often used to delineate hotspots of WVCs, resulting in hotspots that are not spatially independent. We developed a new approach that identifies independent hotspots using attributes of the landscape to inform delineations instead of subjective measures. First, we generated a candidate set of grouping scenarios using unique combinations of kernel-density estimation parameterization (i.e., bandwidth and isopleth values). Next, we associated the groups of …

Genetic Engineering, A Hope For Sustainable Biofuel Production : Review., Sudip Paudel, Michael Menze

Faculty and Staff Scholarship

The use of recently developed genetic engineering tools in combination with organisms that have the potential to produce precursors for the production of biodiesel, promises a sustainable and environment friendly energy source. Enhanced lipid production in wild type and/or genetically engineered organisms can offer sufficient raw material for industrial transesterification of plant-based triglycerides. Bio-diesel, produced with the help of genetically modified organisms, might be one of the best alternatives to fossil fuels and to mitigate various environmental hazards.

Quantitative Trait Loci (Qtl) That Underlie Scn Resistance In Soybean [Glycine Max (L.) Merr.] Pi438489b By ‘Hamilton’ Recombinant Inbred Line (Ril) Population, Kassem My Abdelmajid, Laura Ramos, D. L. Hyten, Jason Bond, Abdelhafid Bendahmane, Prakash R. Arelli, Victor N. Njiti, Silvia Cianzio, Stella K. Kantartzi, Khalid Meksem

Department of Agronomy and Horticulture: Faculty Publications

Soybean cyst nematode caused by Heterodera glycines Ichinohe is the most devastating pest in soybean [Glycine max (L.) Merr.]. Resistance to SCN is complex, polygenic, race and cultivar specific, and it is controlled by several quantitative trait loci (QTL). Our objective was to identify and map QTL for SCN resistance to races 3 (HG Type 0) and 5 (HG Type 2.5.7) using a high density SNP-based genetic linkage map based on the PI438489B by ‘Hamilton’ (PIxH, n=50) recombinant inbred line population. The PI438489B by Hamilton map contained 648 SNPs distributed on 31 LGs with coverage of 1,524.7 cM and …

Lineage-Specific Transcriptional Profiles Of Symbiodinium Spp. Unaltered By Heat Stress In A Coral Host, Daniel J. Barshis, Jason T. Ladner, Thomas A. Oliver, Stephen R. Palumbi

Biological Sciences Faculty Publications

Dinoflagellates of the genus Symbiodinium form an endosymbiosis with reef building corals, in which photosynthetically derived nutrients comprise the majority of the coral energy budget. An extraordinary amount of functional and genetic diversity is contained within the coral-associated Symbiodinium, with some phylotypes (i.e., genotypic groupings), conferring enhanced stress tolerance to host corals. Recent advances in DNA sequencing technologies have enabled transcriptome-wide profiling of the stress response of the cnidarian coral host; however, a comprehensive understanding of the molecular response to stress of coral-associated Symbiodinium, as well as differences among physiologically susceptible and tolerant types, remains largely unexplored. Here, …

Sarpa Salpa Herbivory On Shallow Reaches Of Posidonia Oceanica Beds, Latina Steele, Kelly M. Darnell, Just Cebrián, Jose Luis Sanchez-Lizaso

Biology Faculty Publications

Sarpa salpa herbivory on shallow reaches of Posidonia oceanica beds.— Here, we examined the temporal and small–scale spatial variability of grazing by the herbivorous fish Sarpa salpa on shallow beds of the temperate seagrass Posidonia oceanica. Herbivory intensity expressed as the percent of leaf area taken by fish bites was higher in September 2006 than in February 2007, and at 0.5 m than at 1.5 m during both sampling times. All S. salpa feeding at the shallow locations studied were juveniles, with bite sizes ranging from 0.03 to 0.62 cm2. Juveniles feeding at 1.5 m were larger in February 2007 …

The Effects Of Pknox1.1 Knockdown On Hoxb1a Expression In R4 Of Danio Rerio Hindbrain, Kevin Hess (Class Of 2015)

Writing Across the Curriculum

Zebrafish hindbrain development begins with the folding of the neural plate into the neural tube that gives rise to segments in the hindbrain known as rhombomeres. Each rhombomere gives rise to important structures, such as the otic vesicle and various craniofacial nerves. The first of the rhombomeres to develop is r4—modulated by proper development of hoxb1a. Proper development of r4 triggers a cascade of gene expression of other genes such as fgfs, krox-20­, and many other genes required for proper segmentation of the rest of the hindbrain. Another one of these genes responsible for proper rhombomere segregation is …

Analysis Of Herbicide Treatment Effectiveness On Common Reed (Phragmites Australis) Of Delaware Bay Salt Marshes, Laura Rosa Ruggeri

Theses, Dissertations and Culminating Projects

Management of Phragmites australis (Cav.) Trin. ex Steud., or common reed, an invasive species within the Delaware Bay, U.S. has been ongoing since 1994 as part of the Estuary Enhancement Program (EEP) for Public Service Enterprise Group (PSEG). Phragmites is known to alter the habitat by creating a monoculture, increasing sediment trapping, and decreasing water circulation resulting in decreased biodiversity. Herbicide treatment at EEP Phragmites-dominated sites began as a means to mitigate for loss of nekton species resulting from operations of the Hope Creek-Salem Generating Station once-through cooling system. Using ArcGIS, effectiveness of herbicide treatment was compared at two of …

The Composition And Stability Of The Vaginal Microbiota Of Normal Pregnant Women Is Different From That Of Non-Pregnant Women, Roberto Romero, Sonia S. Hassan, Pawel Gajer, Adi L. Tarca, Douglas W. Fadrosh, Lorraine Nikita, Marisa Galuppi, Ronald F. Lamont, Piya Chaemsaithong, Jezid Miranda, Tinnakorn Chaiworapongsa, Jacques Ravel

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

This study was undertaken to characterize the vaginal microbiota throughout normal human pregnancy using sequence-based techniques. We compared the vaginal microbial composition of non-pregnant patients with a group of pregnant women who delivered at term.

Results

A retrospective case–control longitudinal study was designed and included non-pregnant women (n = 32) and pregnant women who delivered at term (38 to 42 weeks) without complications (n = 22). Serial samples of vaginal fluid were collected from both non-pregnant and pregnant patients. A 16S rRNA gene sequence-based survey was conducted using pyrosequencing to characterize the structure and stability of the vaginal …

Lack Of Sexual Size Dimorphism In Sceloporus Poinsettii From Durango, Mexico, J. A. Lemos-Espinal, Geoffrey R. Smith, John B. Iverson, Juan Manuel Morales Sandoval

Faculty Publications

No abstract provided.