Life Sciences Commons^™

A Study Of The American Black Bear (Ursus Americanus) In Utah: An Analysis Of The Post-Denning Activities And Bear-Human Conflict, Julie Ann Miller

Theses and Dissertations

This study examined two different aspects of black bear (Ursus americanus) ecology in Utah. First, we determined the post-denning behaviors of female black bears in order to help management agencies protect bears from human disturbances as well as set spring hunts that minimize the taking of females with dependent young. We looked at the timing of den emergence (X = 25 March), the number of days at the den site post emergence (X = 11 days), and departure (X = 8 April) for female black bears in Utah from 2011—2013. We also analyzed the effects of cohort (lone …

Generality Of The Terminal Investment Hypothesis: Effects Of Extrinsic Mortality And Resource Availability On Age-Related Reproductive Investment, Allystair Jones

Theses and Dissertations

A central question in life history theory is, what combination of traits and behaviors will lead to the highest reproductive success throughout a lifetime. The trade-off between current and future reproduction is central to the lifetime reproductive success of an organism. If there is a cost to reproduction, then allocation of energy to current reproduction will come at a cost to future reproduction. We expect young individuals to favor future reproduction over current reproduction and that balance shifts to current reproduction as they age (i.e. terminal investment hypothesis). However, how this transition from an emphasis on future reproduction to emphasis …

Understanding The Diversification Of Central American Freshwater Fishes Using Comparative Phylogeography And Species Delimitation, Justin C. Bagley

Theses and Dissertations

Phylogeography and molecular phylogenetics have proven remarkably useful for understanding the patterns and processes influencing historical diversification of biotic lineages at and below the species level, as well as delimiting morphologically cryptic species. In this dissertation, I used an integrative approach coupling comparative phylogeography and coalescent-based species delimitation to improve our understanding of the biogeography and species limits of Central American freshwater fishes. In Chapter 1, I conducted a literature review of the contributions of phylogeography to understanding the origins and maintenance of lower Central American biodiversity, in light of the geological and ecological setting. I highlighted emerging phylogeographic patterns, …

Ground Forces Impact On Release Of Rotational Shot Put Technique, Niklas B. Arrhenius

Theses and Dissertations

In the shot put throw, the primary power is generated in the form of ground reaction forces as a result of action of the lower extremities (Coh, Stuhec, & Supej, 2008). The purpose of this study was to determine how the ground reaction force and ground contact time during the delivery phase of rotational shot put relates to the predicted distance of the throw. This will allow us to determine the optimal approach of force application for maximum throwing distance (Linthorne, 2001). Eight male subjects were used in this study (age 23 ± 4 y; body mass 123 ± 14 …

The Effects Of Worksite Health Promotion Programs On Employee Biometric Data, Jamie M. Pratt

Theses and Dissertations

INTRODUCTION: Worksite health promotion programs (WHPP) promote maintenance and changes of health-related behaviors of employees. Some companies opt to contract with a third party provider to implement a WHPP. PURPOSE: This study evaluated the participation rates, availability and use of health coaching, and changes in biometric data over a 2-year time period of employees in 13 companies for whom the WHPP was implemented by Wellness Corporate Solutions (WCS). METHODS: We had 2 years of biometric, health risk appraisal (HRA), or health coaching data on 4,473 employees. The statistical analysis included biometric screening data (percent body fat, body mass index (BMI), …

Spatial Heterogeneity Of Ecosystem Metabolism In A Shallow Wetland, Daniel Riley Rackliffe

Theses and Dissertations

Spatial heterogeneity in ecosystem metabolism may play a critical role in determining ecosystem functions. Variation in ecosystem metabolism between macrophyte patches in shallow wetlands at the extremes of freshwater habitats has not been investigated. We estimated ecosystem metabolism in mesocosms containing different macrophytes using 24-hour oxygen curves to test our hypotheses: (1) net aquatic production (NAP) during spring and summer would be similar among algal patches (metaphyton and Chara), (2) NAP in algal patches would be greater than patches dominated by the vascular plant Potamogeton foliosus, (3) heterotrophy and anaerobiosis would be greatest in patches dominated by Lemna, and (4) …

A Molecular Phylogeny Of Lampyridae With Insight Into Visual And Bioluminescent Evolution, Gavin Jon Martin

Theses and Dissertations

Fireflies are some of the most captivating organisms on the planet. Because of this, they have a rich history of study, especially concerning their bioluminescent and visual behavior. Among insects, opsin copy number variation has been shown to be quite diverse. However, within the beetles, very little work on opsins has been conducted. Here we look at the visual system of fireflies (Coleoptera: Lampyridae), which offer an elegant system in which to study visual evolution as it relates to their behavior and broader ecology. They are the best-known case of a terrestrial organism that communicates through the use bioluminescence. The …

The Update, December 2014, University Of Northern Iowa. College Of Humanities, Arts And Sciences.

Update

Inside this issue:

-- Harlem Hellfighters: UNI Alumnus Publishes Award Winning Book
-- Department News
-- School of Music Events
-- Science Students Travel to Minneapolis: 50th Anniversary of the Nobel Conference
-- Faculty Research, Scholarship and Creative Activity Awards
-- Graphic Design Students Create Work for the Community: Students Created Work for the Hearst Center for the Arts and Cedar Rock State Park
-- Alumni Spotlight: Department of Chemistry & Biochemistry: Dane Jacobson
-- Alumni Spotlight: Department of Physics: Jack Dostal
-- Alumni Spotlight: Department of Earth Science: Cara Wright
-- Student Spotlight: Department of Philosophy & World Religions: …

Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt

Master's Theses

Packaging Materials account for 31% of the world’s municipal solid waste. Agencies like the Environmental Protection Agency (EPA) and the Agency for Toxic Substances and Disease Registry (ATSDR) are pushing for the increased use of recycled thermoplastic materials. Polyethylene terephthalate (PET) is a commonly recycled thermoplastic which is used to package ready-to-eat fruits and vegetables. Most recycled polyethylene terephthalate (RPET) packaging materials contain heavy metal catalysts, the most common being antimony. The recent increased use of recycled plastic materials has been suspected as the source of increased human heavy metal exposure. In this study, cadmium, chromium, nickel, lead and antimony …

Transcription Of The Streptococcus Pyogenes Hyaluronic Acid Capsule Biosynthesis Operon Is Regulated By Previously Unknown Upstream Elements, Marina Falaleeva, Oliwia W. Zurek, Robert L. Watkins, Robert W. Reed, Hadeel Ali, Paul Sumby, Jovanka M. Voyich, Natalia Korotkova

Molecular and Cellular Biochemistry Faculty Publications

The important human pathogen Streptococcus pyogenes (group A Streptococcus [GAS]) produces a hyaluronic acid (HA) capsule that plays critical roles in immune evasion. Previous studies showed that the hasABC operon encoding the capsule biosynthesis enzymes is under the control of a single promoter, P1, which is negatively regulated by the two-component regulatory system CovR/S. In this work, we characterize the sequence upstream of P1 and identify a novel regulatory region controlling transcription of the capsule biosynthesis operon in the M1 serotype strain MGAS2221. This region consists of a promoter, P2, which initiates transcription of a novel small RNA, HasS, an …

Aminoglycoside-Induced Otoneurotoxicity: Analysis Of Inner Hair Cell Synaptic Plasticity Following Drug Exposure, Matthew M. Abernathy

Dissertations

Aminoglycoside antibiotics are powerful drugs for combating bacterial infections, but are limited in use due to their ototoxicity. This class of drug targets the auditory hair cells of the cochlea, causing cell death, which leads to a decline in auditory function. In spite of much research aimed at revealing a mechanism of damage, there are no co-therapies available to diminish the ototoxic liability of aminoglycosides. Existing research does show that there may be a neurodegenerative process that contributes to the observed toxicity. In an effort towards clarifying present understanding of issue, this dissertation project was conducted to characterize the neurodegenerative …

Ultrafast Interfacial Electron Transfer Across Molecule-Tio2 Nanocomposites: Towards Solar Cells And Two Photon Absorption, Edwin Mghanga

Dissertations

Interfacial charge transfer (ICT) across the molecule-TiO2 nanoparticle interface has gained enormous research attention for applications in dye sensitized solar cells (DSSC), photo-catalysis, water splitting and nonlinear optics. DSSCs are promising clean alternative energy sources. However, current DSSCs suffer from lower efficiencies and higher cost. Better understanding of the ICT processes in DSSCs can help solve these problems. We have used two strategies to understand ICT in the context of DSSCs. Firstly, we used a computationally validated anchor group, acetylacetonate (acac) to bind molecules to the semiconductor surface and facilitate charge separation. Secondly, we used natural dye sensitizers, …

Countrywide Spread Of Oxa-48 Carbapenemase In Lebanon: Surveillance And Genetic Characterization Of Carbapenem-Non-Susceptible Enterobacteriaceae In 10 Hospitals Over A One-Year Period, D. Hammoudi, C. Ayoub Moubareck, J. Aires, A. Adaime, A. Barakat, N. Fayad, N. Hakime, M. Houmani, T. Itani, Z. Najjar, M. Suleiman, R. Sarraf, D. Karam Sarkis

All Works

© 2014 The Authors. Objectives: To detect, characterize, and assess the genetic clonality of carbapenem-non-susceptible Enterobacteriaceae in 10 Lebanese hospitals in 2012. Methods: Selected Enterobacteriaceae isolates with reduced susceptibility to carbapenems were subject to phenotypic study including antibiotic susceptibility, cloxacillin effect, modified Hodge test, and activity of efflux pump inhibitor. Carbapenemase genes were detected using PCR; clonal relatedness was studied by pulsed field gel electrophoresis. Results: Out of 8717 Enterobacteriaceae isolated in 2012, 102 (1.2%) showed reduced susceptibility to carbapenems. Thirty-one (70%) of the 44 studied clinical isolates harbored blaOXA-48, including 15 Klebsiella pneumoniae, eight Escherichia coli, four Serratia marcescens, …

Relationship Between Relative Hive Entrance Position And Dance Floor Location, Chelsea E. Corrigan

Undergraduate Honors Theses

It has been observed that returning honey bee foragers congregate with unemployed foragers and food receiver bees in a localized region of the hive known as the dance floor. Here, the returning foragers advertise food sources via the waggle dance. It was hypothesized that the close proximity of the dance floor to the hive entrance was related to foragers minimizing time and travel inside the hive. The hive entrance is conventionally located at the bottom of the hive. It was suggested that this location was ideal for easy removal of debris. This correlation between dance floor location and hive entrance …

Redesign Of Trans-Splicing Molecules For The Correction Of Dystrophia Myotonica Type 1 Toxic Rna Transcripts, Eleanor G. Harrison

Undergraduate Honors Theses

Dystrophia myotonica (DM1), one of the most common forms of muscular dystrophy, is caused by a repeated trinucleotide expansion in the DMPK gene. This mutation results in the accumulation of toxic cellular RNA transcripts. Spliceosome-mediated RNA trans-splicing (SMaRT) technology is a form of gene therapy that possesses the potential to correct these toxic RNA transcripts and thus cure the disease. Despite its promise, prior research applications of SMaRT technology to DM1 have been hampered by poor efficiency and have not been validated in a relevant model of the disease. In order to improve the efficiency of trans-splicing, this study examined …

Characterization Of Histidine Decarboxylase In Drosophila Using An Internal Flag Epitope, Maxwell Mianecki

Masters Theses

Histamine is a neurotransmitter in arthropods and is responsible for synaptic transmission in vision, mechanosensation, temperature sensing and sleep cycle in Drosophila. Histamine is synthesized by the enzyme histidine decarboxylase (HDC). While histamine is detectable within tissues using current immunofluorescent labeling techniques, immunological approaches have not been successful for HDC itself, with both direct antibodies and terminal epitope tags determined to be ineffective. In order to avoid loss of the epitope tag through putative N-­‐ and C-­‐terminal proteolytic cleavage, known to occur for HDC in other organisms, an internal epitope tag that does not disrupt enzyme function was utilized. A …

The Economic Viability Of Cocoa Crop Insurance In Ghana, Justin D. Mckinley

Graduate Theses and Dissertations

This study was motivated by the fact that Ghanaian cocoa producers face lower yields than other main cocoa producing counties which in turn increases food insecurity for smallholder producers. In addition, low yields experienced by Ghanaian producers is a driving factor for forest degradation and deforestation as cocoa producers encroach further into previously undisturbed forests in efforts to increase their incomes. There are currently production methods to achieve higher yields readily available in Ghana; however, many producers choose not to adopt these methods because they are seen as too risky, or simply cannot adopt them due to financial/credit constraints. A …

Assessing Net Returns To Blueberry Production Using A Decision Support Tool, Sokha Sok

Graduate Theses and Dissertations

The purpose of this study was to develop and assess the profitability of four highbush blueberry farming systems in the south: organic field production, conventional field production, organic high tunnel production and conventional high tunnel production. Four baseline budget scenarios, one for each system, are developed for a 15 year production period. The results suggest that under expected production and price conditions for Northwest Arkansas, while all four production systems generated positive present value of net returns, the conventional field production produced the highest present value of net returns across the 15 years. The breakeven years of production were 7 …

European Consumers' Attitudes Towards Cisgenic Rice, Anne-Cécile Delwaide

Graduate Theses and Dissertations

The enhancement of existing plant breeding techniques, such as cisgenesis, allows plant breeders to enhance an existing cultivar quicker and with little to no genetic drag. Cisgenesis is the genetic modification of a recipient plant with natural gene(s) from a sexually compatible plant. Unlike transgenesis, which is the genetic modification of a recipient plant with gene(s) from any non‐plant organism, or from a donor plant that is sexually incompatible with the recipient plant, the results of cisgenesis could occur naturally over time. Currently, both cisgenic and transgenic products are classified as genetically modified organisms (GMOs) and are labeled as such …

Dialect Use Within A Socially Fluid Group Of Southern Resident Killer Whales, Orcinus Orca, Courtney Elizabeth Smith

Master's Theses

Resident killer whales, Orcinus orca, of the Northeastern Pacific form stable kinship-based matrifocal associations and communicate with group-specific repertoires of discrete calls (dialects) that reflect these associations. The gradual fission of matrilines is usually consistent with dialect variations among groups that may manifest as differences in call usage at the repertoire level or subtle structural differences of the calls themselves. Therefore, matrilines that are more closely related tend to be more acoustically similar. Within the endangered community of Southern Resident killer whales (SRKWs), recent evidence shows that one particular group (L pod) exhibits the lowest rate of intrapod association …

A Phenological Study Of Bat Communities In Southern Mississippi Caves, Zachary Uriah Roth

Master's Theses

Mississippi is generally not known for its caves, and consequently its cave flora and fauna remain largely unstudied. From fall 2010 to winter 2013, we studied the bat populations in the three largest caves in Mississippi. The most common (and only) species found in these caves were Myotis austroriparius and Perimyotis subflavus. I collected monthly data on the number of bats per species, behaviors and locations of the bats within the caves, as well as atmospheric data at selected positions within each cave. All three caves were found to have significant temperature differences between seasons (winterPerimyotis subflavus was found …

Exploring The Glucocorticoid Actions Of 1Α-Hydroxycorticosterone (1Α-Oh-B) In The Elasmobranch Fishes, Faith Nichole Lambert

Master's Theses

The corticosteroid 1α-hydroxycorticosterone (1α-OH-B) is unique to the elasmobranch fishes. It is thought that 1α-OH-B acts as both the primary glucocorticoid (GC) and mineralocorticoid (MC) in these fishes, a dual role analogous to that of cortisol in the teleost fishes. The MC characteristics of 1α-OH-B are well supported, but data supporting its GC functions are lacking. In this study, the putative GC actions of 1α-OH-B were examined. The first experiment characterized the physiological stress response of the Atlantic stingray (Dasyatis sabina) to air exposure, with particular regards to the roles of corticosteroids and metabolic fuels. Results demonstrate that …

Effects Of A Nutrition Education Intervention On Inner City Children, Stephanie P. Windle

Honors Theses

This paper focuses on nutrition education research conducted between February and April 2014. The researcher’s goal was to determine the effectiveness of a nutrition education intervention on low-income children. The USDA and Academy of Nutrition and Dietetics provided most of the research used to create the intervention. The definition of “healthy” used for this study was defined by the USDA (2014) as “whole foods such as fruits, vegetables, whole grains, fat free or low-fat dairy, and lean meats that are perishable (fresh, refrigerated, or frozen) or canned as well as nutrient-dense foods and beverages encouraged by the 2010 Dietary Guidelines …

Modulation Of The Ovarian Cancer Humoral Response By Tumor-Derived Exosomes., Carolyn Denise Roberson

Electronic Theses and Dissertations

The pathogenesis of epithelial ovarian cancer is complicated by its diagnosis during the latter stages of the disease resulting from lack of symptoms or from presence of symptoms that mimic other conditions. Because of the often fatal prognosis by the time of actual detection, efforts are being made to better understand the host immune response to ovarian cancer. In this study, the contributions of the humoral immune response were investigated by focusing on the role of tumor-derived exosomes and their ability to modulate humoral immune responses. First, ovarian cancer patient-derived free circulating antibodies were investigated for immunoreactivity to patient antigen. …

Novel Posttranslational Modification In Lkb1 Activation And Function, Szu-Wei Lee

Dissertations and Theses (Open Access)

Cancer cells display dramatic alterations in cellular metabolism to meet their needs of increased growth and proliferation. In the last decade, cancer research has brought these pathways into focus, and one emerging issue that has come to attention is that many oncogenes and tumor-suppressors are intimately linked to metabolic regulation (Jones and Thompson, 2009). One of the key tumor-suppressors involved in metabolism is Liver Kinase B1 (LKB1). LKB1 is the major upstream kinase of the evolutionarily conserved metabolic sensor—AMP-activated protein kinase (AMPK). Activation of the LKB1/AMPK pathway provides a survival advantage for cells under energy stress. LKB1 forms a heterotrimeric …

Insulin Resistance And Muscle Insulin Receptor Substrate-1 Serine Hyperphosphorylation, Charles A. Stuart, Mary E. A. Howell, Brian M. Cartwright, Melanie P. Mccurry, Michelle L. Lee, Michael W. Ramsey, Michael H. Stone

ETSU Faculty Works

Insulin resistance in metabolic syndrome subjects is profound in spite of muscle insulin receptor and insulin-responsive glucose transporter (GLUT4) expression being nearly normal. Insulin receptor tyrosine kinase phosphorylation of insulin receptor substrate-1 (IRS-1) at Tyr896 is a necessary step in insulin stimulation of translocation of GLUT4 to the cell surface. Serine phosphorylation of IRS-1 by some kinases diminishes insulin action in mice. We evaluated the phosphorylation status of muscle IRS-1 in 33 subjects with the metabolic syndrome and seventeen lean controls. Each underwent euglycemic insulin clamps and a thigh muscle biopsy before and after 8 weeks of either strength or …

An Exploratory Study On The Use Of Concentric Velocities In The Back Squat As A Monitoring Tool, Kevin M. Carroll, Josh D. Christovich, Caleb D. Bazyler, Nicholas J. Fiolo, George K. Beckham, Kimitake Sato

ETSU Faculty Works

Abstract available in the 9th Annual Coaches and Sport Science College .

The Mechanism Of Lhcp Insertion Into Thylakoid Membranes, Larae Brown

Graduate Theses and Dissertations

The light harvesting chlorophyll a/b-binding proteins (LHCPs) are the most abundant membrane proteins. LHCP is a nuclear encoded protein which is targeted to the thylakoid membranes by chloroplast signal recognition particles (cpSRP). Insertion into thylakoid membranes is facilitated by the cpSRP receptor cpFtsY and the Alb3 translocase. Work here focused on understanding the molecular events of LHCP insertion into the thylakoid membranes. Specifically, we sought to develop a tool to detect the insertion of the lumen-localized loop of LHCP into thylakoid membranes, which relies on cleavage of the loop by a thylakoid lumen processing protease. We also sought to understand …

Reconciliation Ecology: A New Paradigm For Advancing Creation Care, Dave P. Warners, Michael Ryskamp, Randall Van Dragt

University Faculty Publications and Creative Works

Current global environmental challenges—species loss, overconsumption, climate change, and others—have not been countered in the faith community with a response worthy of their signifi cance. While the prevailing faith-based creation-care paradigm, environmental stewardship, has been invaluable in moving us beyond the utilitarian notion of “dominion,” the stewardship concept does not suffi ciently emphasize our embedded, dependent relationship with the creation. To better represent our creationcare responsibilities, we propose a new paradigm based on a model of servanthood and informed by the concept of reconciliation ecology, which focuses on mending broken relationships between human beings and nonhuman creation. Drawing from the …

Linking The Green And Brown Worlds: The Prevalence And Effect Of Multichannel Feeding In Food Webs, Elizabeth M. Wolkovich, Stefano Allesina, Kathryn L. Cottingham, John C. Moore, Stuart A. Sandin, Claire De Mazancourt

Dartmouth Scholarship

Recent advances in food‐web ecology highlight that most real food webs (1) represent an interplay between producer‐ and detritus‐based webs and (2) are governed by consumers which are rampant omnivores; feeding on varied prey across trophic levels and resource channels. A possible avenue to unify these advances comes from models demonstrating that predators feeding on distinctly different channels may stabilize food webs. Empirical studies suggest many consumers engage in such behavior by feeding on prey items from both living‐autotroph (green) and detritus‐based (brown) webs, what we term “multichannel feeding,” yet we know little about how common such feeding is across …