Life Sciences Commons^™

Identifying Polyglutamine Protein Species In Situ That Best Predict Neurodegeneration, Jason Miller, Montserrat Arrasate, Elizabeth Brooks, Clare Peter Libeu, Justin Legleiter, Danny Hatters, Jessica Curtis, Kenneth Cheung, Preethi Krishnan, Siddhartha Mitra, Kartika Widjaja, Benjamin A. Shaby, Gregor P. Lotz, Yvonne Newhouse, Emily M. Sontag, Alex Osmand, Michelle Gray, Vanitha Thulasiramin, Frederic Saudou, Mark Segal, X William Yang, Eliezer Masliah, Leslie M. Thompson, Paul J. Muchowski, Karl H. Weisgraber, Steven Finkbeiner

Biological Sciences Faculty Research and Publications

Polyglutamine (polyQ) stretches exceeding a threshold length confer a toxic function to proteins that contain them and cause at least nine neurological disorders. The basis for this toxicity threshold is unclear. Although polyQ expansions render proteins prone to aggregate into inclusion bodies, this may be a neuronal coping response to more toxic forms of polyQ. The exact structure of these more toxic forms is unknown. Here we show that the monoclonal antibody 3B5H10 recognizes a species of polyQ protein in situ that strongly predicts neuronal death. The epitope selectively appears among some of the many low-molecular-weight conformational states assumed by …

Can Antioxidant-Rich Berries Improve Risk Factors Associated With Cardiovascular Disease In Postmenopausal Smokers?, Jordan Alaine Teeple

Graduate Theses and Dissertations

The purpose of this study was to examine the effects of smoking on risk factors associated with cardiovascular disease (CVD) and determine the efficacy of antioxidant-rich berries in ameliorating these risk factors in postmenopausal smokers. Menopause and cigarette smoking have been identified as major risk factors for CVD due to a decrease in antioxidant protection, increase in inflammation and oxidative stress, and adverse changes in serum lipids. Antioxidants, specifically flavonoid compounds found in blackberries and blueberries, have been shown to prevent low-density lipoprotein cholesterol oxidation and thus prevent downstream inflammation and oxidative stress. Healthy, postmenopausal nonsmokers (n=14) and smokers (n=31) …

In Vitro Selection Of Aptamers Against Avian Influenza Virus H5n1, Jingjing Zhao

Graduate Theses and Dissertations

Over $10 billion losses in the poultry industry were caused by avian influenza (AI) so far. Rapid and specific detection of avian influenza virus is urgently needed with the concerns over the outbreaks of highly pathogenic H5N1 influenza virus and cases of animal and human infection. Aptamers are oligonucleic acid or peptide molecules that bind a specific target molecule with good affinity. They show better thermal stability than antibodies. The goal of this research was to select DNA-aptamers as the specific recognition element of AI H5N1virus to be used in detection assays specific for field application. In this study, Systematic …

Improvements On Just-About-Right (Jar) Scales As Product Optimization Tools Using Kano Modeling Concepts, Bangde Li

Graduate Theses and Dissertations

Attribute Kano characteristics are useful in product design to prioritize development efforts. However, attribute Kano characteristics have not been discussed and applied to product optimization when using Just-About-Right (JAR) scales. Product optimizations without identifying attributes Kano characteristics can be misleading. The two objectives in this research were: 1. Determine attribute Kano categories using a modified classic Kano classification methodology. 2. Propose a method to measure attribute performance and identify attribute Kano characteristics to direct product optimization. Two methodologies of attribute Kano classification were investigated. In experiment one, a modified classic Kano methodology was employed to determine attribute Kano categories through …

Effect Of Nutrient Supplements On Cucumber Fermentation By Lactic Acid Bacteria, Shruti Tripuraneni

Graduate Theses and Dissertations

Lactic acid bacteria (LAB) are important industrial microorganisms involved in fermentation of food and beverage products. The strict fermentative growth of LAB has complex requirements of various nutrients including amino acids, vitamins and minerals. Information about the effect of these nutrients on the growth of LAB in cucumber fermentation is not readily available. It is evident, from previous research that certain nutrients like; leucine, isoleucine, tryptophan, valine, biotin, nicotinic acid, pantothenic acid, riboflavin, manganese and magnesium are beneficial for LAB growth, but are not provided in sufficient quantities by the cucumber in the brine. The objective of this study was …

Analyzing Spring Freeze Impacts On Deciduous Forest Productivity Using Modis Satellite Imagery, Karl Lintvedt

Graduate Theses and Dissertations

The impacts of an April 2007 spring freeze event on the productivity of deciduous broadleaf forest were analyzed using geographic information system (GIS) tools. Forest productivity was modeled using the Enhanced Vegetation Index (EVI), as recorded by the Moderate Resolution Imaging Spectroradiometer (MODIS) satellite sensor. Measures of spatial autocorrelation were used to quantify the degree of spatial congruence between a map depicting the severity of the freeze event, and maps modeling forest productivity throughout the year. The results show a geographic correlation between the unseasonably low minimum temperatures sustained during the freeze and the unusually low forest productivity that followed. …

Detection Of Two Pathogens Of High Importance To The National Poultry Improvement Plan: Salmonella Spp. And Mycoplasma Spp., Robin Levi Jarquin

Graduate Theses and Dissertations

In 1935, the National Poultry Improvement Plan (NPIP) was created to control Salmonella gallinarum and Salmonella pullorum . These two pathogens were devastating economically for poultry producers. Through cooperative efforts using vaccination and strict biosecurity, these two pathogens were eradicated from the United States. Currently, the NPIP program is targeting two other poultry pathogens, Salmonella enterica serovar Enteritidis and Mycoplasma . In the broiler industry it targets 2 specific Mycoplasma species (synoviae, gallisepticum). Vaccinations for these bacteria are available, but are not fully effective at controlling all strains and serovars. Thus, constant monitoring systems and strict biosecurity measures are necessary …

Proteome Based Development Of Novel Affinity Tail For Immobilized Metal Affinity Chromatography And Hydrophobic Interaction Chromatography, Neha Tiwari

Graduate Theses and Dissertations

At industrial scale, reducing the step in purification and recovery is desired; this not only decreases the cost but also increases the yield. Hydrophobic Interaction Chromatography (HIC) and Immobilized Metal Affinity Chromatography (IMAC) both are not harsh on biological structure or activity of proteins; also both the techniques are economical and therefore a suitable choice at industrial level. This dissertation comprises of three parts. Purpose for the Part I was to identify and characterize Escherichia coli proteins which display affinity towards both IMAC and Hydrophobic Interaction Chromatography (HIC). Co (II) IMAC was chosen as the primary capture step, followed by …

Earthworms Of Arkansas And Contributions Of Earthworm Evolutionary Origin And Ecological Group To Nitrogen Cycling In A Model Soil And Tall Fescue System, Peter John Tomlinson

Graduate Theses and Dissertations

Evolutionary origin and ecological niche may impact how earthworm species affect N cycling. Tall fescue (Lolium arundinaceum (Schreb) S. J. Darbysh) infection with the toxic endophyte Neotyphodium coenophialum Glenn, Bacon & Hanlin (E+) may influence earthworm communities in soil compared to non-toxic endophyte (NE+) infected tall fescue. Conversely, different earthworms may contribute different amounts of N to E+ vs. NE+ tall fescue. A field survey was conducted from January 2007 to January 2008 to determine the impact of E+ and NE+ tall fescue, planting date, and seasonal variables, e.g. soil temperature and moisture, on earthworm species, abundances, and population …

Effect Of Supplemental Trace Mineral Source (Organic Versus Inorganic) On Bull Semen Quality, Matt Rowe

Graduate Theses and Dissertations

Studies indicate that organic forms of trace minerals can improve cow reproductive performance, particularly during periods of stress. However, limited information is available on the effects of organic trace mineral supplementation on bull fertility. The objective of this study was to evaluate the effect of organic versus inorganic trace mineral supplementation on bull semen quality before and after freezing, as measured by computer-assisted sperm analysis (CASA). Angus and Balancer bulls were assigned to inorganic (n = 9) and organic (n = 10) trace mineral treatments, based on initial semen quality, breed, body weight, and age. The bulls were maintained in …

Determination Of Poultry Litter's Nitrogen-Fertilizer Value For Winter Wheat Production, Brett Lee Gordon

Graduate Theses and Dissertations

Nitrogen (N) availability in poultry litter (PL) applied in the fall as a nutrient source for winter wheat (Triticum aestivum L.) has not been adequately characterized. Our objective was to determine a potentially available N (PAN) coefficient based on the inorganic-N fertilizer equivalence (FEQ) of fall-applied PL to winter wheat via field and laboratory research. Wheat grown at six site-years received 0, 84 and 168 kg N ha-1 as PL applied preplant and/or at-planting. The FEQ of PL-N was based on growth and yield responses of wheat fertilized with 22 to 191 kg N ha-1 applied in late winter as …

Environmental Influences On Juvenile Fish Abundances In A River-Dominated Coastal System, Laure Carassou, Brian Dzwonkowski, Frank J. Hernandez, Jr., Sean P. Powers, William M. Graham, Kyeong Park, John Mareska

University Faculty and Staff Publications

We investigated the influence of climatic and environmental factors on variations in juvenile abundances of marine fishes in a river-dominated coastal system of the north-central Gulf of Mexico, where an elevated primary productivity sustains fisheries of high economic importance. Fish were collected monthly with an otter trawl at three stations near Mobile Bay from 1982 to 2007. Fish sizes were used to isolate juvenile stages within the data set, and monthly patterns in juvenile fish abundance and size were then used to identify seasonal peaks for each species. The average numbers of juvenile fish collected during these seasonal peaks in …

The Marketplace Of Boston: Macrobotanical Remains From Faneuil Hall, Ciana Faye Meyers

Graduate Masters Theses

Residents of Boston in the eighteenth century utilized a wide range of botanical materials in their daily lives, navigating complex urban marketing systems and utilizing their own individual ingenuity to procure botanical resources. The one thousand eight hundred and eighty-three botanical remains recovered from a "community midden" underneath the present-day Faneuil Hall represents a diverse collection of taxa which encodes information not only about the localized dietary practices of colonial urban residents, but also helps to illuminate the more subtle ramifications of Boston's participation in the Atlantic economy on the lives of its residents. These botanical remains represent taxa from …

Bumble Flower Beetle, Taun Beddes, Ryan S. Davis

All Current Publications

The bumble flower beetle (BFB) is a common member of the Scarabaeidae (scarab) beetle family. Its common name originated because adult BFB’s often fly close to the ground and emit a loud buzzing sound similar to that of a bumble bee. Larvae (grubs) and adults are often found in or near fermenting and/or decomposing organic matter.

Corn Earworm, Diane G. Alston, Shawn Olsen, James Barnhill

All Current Publications

In Utah, there are typically three generations of corn earworm (CEW) each year. The first generation of adults either come from overwintering pupae (southern and central Utah), or migrate into northern Utah. The adult moth is tannish brown with a 1 1/2 inch wingspan. The front wings are marked with a distinct dark spot in the center and darker bands near the outer margins. The hind wings are lighter tan, with a dark band along the outer margins. The male moths have green eyes. Moth flight occurs primarily on warm, overcast evenings. Moth populations can be monitored using pheromone or …

Hemipteran Beneficiosos: Damsel Bugs, Ricardo Ramirez, Ron Patterson, Forest Young

All Current Publications

Los insectos depredadores pueden proveer un control de pestes natural al comer insectos no deseados. Este servicio beneficioso se llama control biológico. Los insectos depredadores son una de las razones por las que las poblaciones de pestes pueden mantenerse a niveles no-dañinos.

Spruce Health In Utah Landscapes, Ryan S. Davis, Michael R. Kuhns, Claudia Nischwitz

All Current Publications

Spruces are common trees in cultivated landscapes in Utah. They have varied shapes, attractive foliage color, and can be fairly long-lived. They have pests, but not overly so, and are not very messy.

Termitas Subterraneas, Erin Hodgson, Alan H. Roe

All Current Publications

Las termitas subterráneas son insectos sociales con un sistema de castas, y pertenecen al orden Isoptera y a la familia Termitidae. Estas termitas están relacionadas cercanamente a termitas de madera seca (Kalotermitidae) y de madera húmeda (Termopsidae).

Western Flower Thrips (Frankliniella Occidentalis), L. Irene Terry, Diane Alston

All Current Publications

Western flower thrips (WTF; Thysanoptera: Thripidae) are native to western North America and are widespread throughout this region.

Selecting And Using Inorganic Fertilizers, Rich Koenig, Larry Rupp

All Current Publications

This publication analyzes the selection and use of inorganic fertilizers.

Selecting And Using Organic Fertilizers, Mike Johnson, Rich Koenig

All Current Publications

This publication gives insight into organic fertilizer types and their nutrient values.

Getting Chemicals Into Trees Without Spraying, Michael R. Kuhns

All Current Publications

Chemicals are applied to trees for many reasons. Insecticides repel or kill damaging insects, fungicides treat or prevent fungal diseases, nutrients and plant growth regulators affect growth, and herbicides kill trees or prevent sprouting after tree removal. Spraying is the most typical way to apply these chemicals. It is fast, uses readily available equipment, and is understood. The down side of spraying is that much of the chemical being applied is wasted, either to drift, run off, or because it can not be applied precisely to where it is needed in the tree. Also, in many cases the chemical is …

Sunscald Injury Or Southwest Winter Injury On Deciduous Trees, Katie Wagner, Michael R. Kuhns

All Current Publications

Sunscald or southwest winter injury presents a significant threat to the trunks and limbs of certain deciduous trees. Although it can happen on shrubs it is more common on trees.

Chinches De Cama (Bed Bugs): Para Profesionales En El Control De Pestes, Ryan S. Davis

All Current Publications

En la última década, reportes de infestaciones de chinches de cama (Cimicidae: Cimex lectularius) han estado en aumento en todo Norte América y en otros países. De acuerdo con esto, las presentaciones de chinches de cama al Utah Plant Pest Diagnostic Lab también han aumentado. Esta hoja de datos ha sido diseñada para informar a los profesionales en el control de pestes de las complejidades involucradas en la erradicación de chinches de cama.

A Comparison Of Intensive Care Unit Mortality Prediction Models Through The Use Of Data Mining Techniques, Sujin Kim, Woojae Kim, Rae Woong Park

Institute for Biomedical Informatics Faculty Publications

OBJECTIVES: The intensive care environment generates a wealth of critical care data suited to developing a well-calibrated prediction tool. This study was done to develop an intensive care unit (ICU) mortality prediction model built on University of Kentucky Hospital (UKH)'s data and to assess whether the performance of various data mining techniques, such as the artificial neural network (ANN), support vector machine (SVM) and decision trees (DT), outperform the conventional logistic regression (LR) statistical model.

METHODS: The models were built on ICU data collected regarding 38,474 admissions to the UKH between January 1998 and September 2007. The first 24 hours …

Escape From Competition: Neighbors Reduce Centaurea Stoebe Performance At Home But Not Away, Ragan M. Callaway, Lauren P. Waller, Alecu Diaconu, Robert Pal, Alexandra R. Collins, Heingz Mueller-Schaerer, John L. Maron

Biological Sciences Faculty Publications

The greater abundance of some exotic plants in their nonnative ranges might be explained in part by biogeographic differences in the strength of competition, but these competitive effects have not been experimentally examined in the field. We compared the effects of neighbors on the growth and reproduction of spotted knapweed (Centaurea stoebe) in Europe, where it is native, and in Montana, where it is invasive. There were strong negative competitive effects of neighboring vegetation on C. stoebe growth and reproduction in Europe. In contrast, identical experiments in Montana resulted in insignificant impacts on C. stoebe. Although the …

Genetic Monitoring For Managers: A New Online Resource, Jeffrey Brian Stetz, Katherine Kendall, Christina D. Vojta, Genetic Monitoring (Gem) Working Group

Wildlife Biology Faculty Publications

Monitoring our natural resources will increasingly rely on genetic tools in order to understand and respond to invasive species, habitat degradation, fragmentation, disease, or climate-related changes. In recent years, the rapidly evolving field of genetic monitoring has seen explosive growth in sampling methods, genetic markers, and analytical approaches designed to estimate a wide range of parameters from connectivity to population growth rates. Some of these methods have taken root and now dominate particular aspects of population assessment and monitoring, whereas others have seen less success in real-world applications. To aid managers and researchers with limited genetics experience, we developed a …

Maternal Separation Affects Dopamine Transporter Function In The Spontaneously Hypertensive Rat: An In Vivo Electrochemical Study, Jacqueline S. Womersley, Jennifer H. Hsieh, Lauriston A. Kellaway, Greg A. Gerhardt, Vivienne A. Russell

Neuroscience Faculty Publications

BACKGROUND: Attention-deficit/hyperactivity disorder (ADHD) is a developmental disorder characterised by symptoms of inattention, impulsivity and hyperactivity. The spontaneously hypertensive rat (SHR) is a well-characterised model of this disorder and has been shown to exhibit dopamine dysregulation, one of the hypothesised causes of ADHD. Since stress experienced in the early stages of life can have long-lasting effects on behaviour, it was considered that early life stress may alter development of the dopaminergic system and thereby contribute to the behavioural characteristics of SHR. It was hypothesized that maternal separation would alter dopamine regulation by the transporter (DAT) in ways that distinguish SHR …

Comparing Cropping System Productivity Between Fixed Rotations And A Flexible Fallow System Using Modeling And Historical Weather Data In The Semi-Arid Central Great Plains, Juan Jose Miceli-Garcia

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

In the Central Great Plains, the predominant crop rotation is winter wheat (Triticum aestivum L.)-fallow. Producers are looking to add diversity and intensity to their cropping systems by adding summer crops, however, the elimination of summer fallow may increase crop production risk. The objective of this study was to use crop simulation modeling to compare the productivity of two fixed rotations [winter wheat-corn (Zea mays L.)-fallow and winter wheat-corn-spring triticale (X Triticosecale Wittmack)] with simulated flexible fallow rotations. The flexible fallow rotations made the decision to plant triticale or use summer fallow prior to winter wheat seeding based …

Characterization Of The Tolerance Response In The Soybean Ks4202 To Aphis Glycines Matsumura, Travis J. Prochaska

Department of Entomology: Dissertations, Theses, and Student Research

Since the introduction of the soybean aphid, Aphis glycines Matsumura, to the soybean growing regions of the United States, the soybean aphid has caused considerable economic damage and yield loss to soybean growers. The objectives of this research were to evaluate selected genotypes for resistance to the soybean aphid and characterize transcriptional changes in response to aphid feeding to better understand the underlying tolerant mechanism(s) in KS4202 and genes contributing to its tolerance response. A field study (2009) was conducted to evaluate selected soybean genotypes during their reproductive stages for resistance to A. glycines. The economic injury level (EIL) …