Life Sciences Commons^™

Low Endemism, Continued Deep-Shallow Interchanges, And Evidence For Cosmopolitan Distributions In Free-Living Marine Nematodes (Order Enoplida), Holly M. Bik, W. Kelley Thomas, David H. Lunt, P. John D. Lambshead

Hubbard Center for Genome Studies (HCGS)

Background: Nematodes represent the most abundant benthic metazoa in one of the largest habitats on earth, the deep sea. Characterizing major patterns of biodiversity within this dominant group is a critical step towards understanding evolutionary patterns across this vast ecosystem. The present study has aimed to place deep-sea nematode species into a phylogenetic framework, investigate relationships between shallow water and deep-sea taxa, and elucidate phylogeographic patterns amongst the deep-sea fauna. Results: Molecular data (18 S and 28 S rRNA) confirms a high diversity amongst deep-sea Enoplids. There is no evidence for endemic deep-sea lineages in Maximum Likelihood or Bayesian phylogenies, …

From Seed To Harvest: A Comparative Life Cycle Assessment Of Conventional Vs. Organic Cotton Agriculture, Rebecca Hoehn

Masters Theses, 2010-2019

Conventional and organic cotton farming practices both have benefits and detriments to each type. Cotton agriculture is responsible for the use of a third of the agriculture industries’ pesticide use. In order to help alleviate the use of pesticide dependency the following study reviews the use of pesticides as well as other stressors of both systems and hopes to decide how sustainable agriculture in the cotton industry can be attained. Evaluations of both systems have been compared in order to reach the decisions of sustainability.

The Role Of Quality Labels In Farming Diversification And Rural Development, Eman Vella

Masters Theses, 2010-2019

The European Union promotes marketing of quality food products through a quality labelling scheme having three labels known as PDO, PGI and TSG. Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) are two labels that protect products with an association to a particular region. Such products need to have a traditional link with the area as well as unique attributes (known as specifications) that make the product different from the customary. PDO labels designate a product that is linked with an area in every aspect, while PGI labels indicate that the product has a unique geographical link in …

Sustainable Agricultural Management And Landscaping Through Agroforestry And Permaculture Case Study: Northern Malta, Shawn Sammy Vella

Masters Theses, 2010-2019

The farming industry in Malta is facing severe challenges. Many small patches of arable land are being abandoned following land use conflicts and competition from foreign agricultural products. This reduces the economic capacity of the farming industry which leads to soil erosion and land degradation. On the other hand, the remaining land is being intensively farmed with the use of chemical fertilisers and pesticides. Local government following EU regulations is trying to change this trend by aiming more at promoting a sustainable future for this industry in Malta whilst preserving the natural environments, but still many farmers choose not to …

Modulation Of Alpha-Subunit Visit-Dg Sequence Residues Ser-347, Gly-351 And Thr-349 In The Catalytic Sites Of Escherichia Coli Atp Synthase., Laura Elaine Brudecki

Electronic Theses and Dissertations

Binding of inorganic phosphate (P_i) in ATP synthase catalytic sites is a crucial step for the synthesis of adenosine-5'-triphosphate (ATP). ATP is the fundamental means of cellular energy in almost every organism, and in order to gain insight into the regulation of ATP catalysis, critical amino acid residues responsible for binding P_i must be identified. Here, we investigate the role of highly conserved α-subunit VISIT-DG sequence residues αSer-347, αGly-351, and αThr-349 in P_i binding. Mutations αS347A/Q, αG351Q, αT349A/D/R, βR182A, and αT349R/βR182A were generated via site directed mutagenesis. Results from biochemical assays showed that αSer-347 is required …

Sensitivity And Exposure Of Brook Trout (Salvelinus Fontinalis) Habitat To Climate Change, Bradly Allen Trumbo

Masters Theses, 2010-2019

Predicting coldwater fisheries distributions under various climate scenarios is of interest to many fisheries managers and researchers. Larger scale models have been useful in highlighting the potential large scale threat. However, the error associated with these models makes predictions of the persistence of individual cold water fisheries problematic. Most of this error is associated with predicted air and water temperatures which typically are simple elevation and location (latitude/longitude) models with simple caveats such as 1°C increase in air temperature equals 0.8°C increase in water temperatures. I directly measured paired air and water temperatures in watersheds containing reproducing populations of brook …

The Effects Of Nutrient Availability And Season On The Somatotropic Axis In Free-Ranging Alaskan Moose (Alces Alces), Amanda A. Parillo

Master's Theses

No abstract provided.

Molecular Evidence Suggests Multiple Evolutionary Origins Of Sociality In The Polyphenic Spider Anelosimus Studiosus (Araneae: Theridiidae)., Nathaniel O. Weber

Electronic Theses and Dissertations

Anelosimus studiosus exhibits two behavioral phenotypes: subsocial and social. This is the only documented spider inhabiting a temperate climate exhibiting social behavior. While the subsocial phenotype is most common throughout the range, the social behavior occurs in isolated pockets in northern latitudes. This study examines the origins of the social phenotype within a segment of the spider's range. Two hypotheses are tested: 1) pockets of social behavior represent a single origin or 2) pockets of social behavior represent local evolutions, thus leading to multiple origins of evolution. Microsatellite loci were used to determine genetic structure of the population and to …

Discovery And Characterization Of An Antibiotic From The Soil Bacterium Bacillus Sp., Thomas S. Barber

Electronic Theses and Dissertations

Many important antibiotics have become nearly obsolete due to the rise of antibiotic resistant pathogens. Rhodococcus, an actinomycete related to the prolific antibiotic producing genus Streptomyces, harbors over 30 genes for secondary metabolism that could be involved in antibiotic production. Several antibiotics have already been reported for Rhodococcus, suggesting the genus may be a good source for new inhibitory compounds. Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 Rhodococcus) and screened for antibiotic producers. BTHX2, a species of Bacillus was found to have activity against Micrococcus luteus and Rhodococcus erythropolis. BTHX2 …

Lack Of Rhythmicity In The Honey Bee Queen: An Investigation Of Temporal Behavioral Patterns In Apis Mellifera Ligustica., Jennifer N. Johnson

Electronic Theses and Dissertations

Little is known about the behavioral patterns of honey bee queens. To determine if mated honey bee queens possess diel rhythmicity in behavior, we observed them in glass-sided observation hives using three types of observation regimes: focal studies consisting of 2-hour and 24-hour continuous observations as well as scan-sampling of multiple queens. All behaviors (active: walking, inspecting, egg-laying, begging for food, feeding, and grooming self; inactive: standing) occurred at all times of day and night, but no queen showed consistent diel rhythmicity in any of the individual behaviors. There were no consistent diel differences in active versus inactive behaviors or …

A Profile Of Rural Southern Appalachian Hiv Patients: Vamc Versus Com., Lauren Brooks

Undergraduate Honors Theses

Although the AIDS epidemic in America began approximately three decades ago, much remains to be learned about the epidemiology of human immunodeficiency virus (HIV) infections in rural America. This study compared profiles of HIV patients at the James H. Quillen Veterans Affairs Medical Center (VAMC) in Mountain Home, TN to those seen at a university-based community HIV care clinic (COM) in Johnson City, TN.

Neurochemical Levels Correlate With Population Level Differences In Social Structure And Individual Behavior In The Polyphenic Spider, Anelosimus Studiosus., Jennifer Bryson Price

Electronic Theses and Dissertations

Anelosimus studiosus is a socially polyphenic spider. Individuals can be classified as social/tolerant or solitary/aggressive. These behavioral differences are associated with considerable variation in social structure. Here, we begin to examine the physiological differences that may underlie the behavioral dimorphism in this species and possible implications for the evolution of sociality. Octopamine is a neurotransmitter that has been found to elevate aggression in invertebrates. Serotonin has been shown, in some cases, to interact antagonistically with octopamine. We used High Pressure Liquid Chromatography with Electrochemical Detection to quantify levels of these neurochemicals among adult females from social (multi-female) and solitary (single-female) …

Mosaic Analysis With Double Markers (Madm) As A Method To Map Cell Fates In Adult Mouse Taste Buds., Preston D. Moore

Electronic Theses and Dissertations

Taste buds are chemosensory endorgans embedded in the oral epithelium composed of cells that undergo continuous replacement. Mature taste cells live on average 10-14 days and are replaced by new cells when they die. However, the mechanism by which taste cells are produced and integrated into the taste bud as mature taste cells remains unknown. Previous studies approached this issue from either cell cycle gene expression properties or lineage tracing of precursor cells. In our study, we apply a new fate mapping technique that combines these two ideas. This technique, Mosaic Analysis with Double Markers, allows for simultaneous gene knockout …

Systemic And Microcirculatory Effects Of Hboc-201: A Hemoglobin-Based Oxygen Carrier, Bjorn Song

Theses and Dissertations

Top-loading This study compared the effects on oxygen (O2) transport of four fluids: 5.9% human serum albumin (HSA) a non-O2 carrying iso-oncotic solution (volume control);HBOC-201 (Hemopure, Biopure Corp., Cambridge, MA); MP50, HBOC-201 with a P50 of 18 mmHg; and lastly LP50, an HBOC-201 with P50 of 17 mmHg and higher viscosity (4 cP). It has been proposed that HBOC with a higher O2 affinity and a viscosity closer to that of whole blood will cause less vasoconstriction and thus a lower MAP than Hemopure (P50 = 40 mmHg and 2.2 cP). Intravital microscopic measurements were made on the spinotrapezius muscle …

Replacement Of Grazed Forage With Wdgs And Poor Quality Hay And Straw Mixtures, Sandra B. Villasanti

Department of Animal Science: Dissertations, Theses, and Student Research

A grazing study was conducted at the University of Nebraska Gudmundsen Sandhills Laboratory from mid-June to late-August across two years (2009 and 2010) to evaluate the effects of supplementation with mixtures of wet distillers grains (WDGS) and straw or hay on grazed forage intake. Twenty 1 ha paddocks replicated over two blocks were randomly assigned to one of four treatments: Control (CON) at the recommended stocking rate (1.68 AUM/ha in 2009 and 1.64 AUM/ha in 2010), and three double stocked treatments supplemented with 60% straw and 40% WDGS (STRAW), 60% hay and 40% WDGS (LOW), and 70% hay and 30% …

Bioremediation Of Arsenic Contaminated Soils Using Rudbeckia Hirta And Arbuscular Mycorrhizal Fungi, Michael Shaw

Honors Theses

No abstract provided.

H-Ns Binding And Repression Of The Ctx Promoter In Vibrio Cholerae, Emily A. Stonehouse, Robin R. Hulbert, Melinda B. Nye, Karen Skorupski, Ronald K. Taylor

Dartmouth Scholarship

Expression of the ctx and tcp genes, which encode cholera toxin and the toxin coregulated pilus, the Vibrio cholerae O1 virulence determinants having the largest contribution to cholera disease, is repressed by the nucleoid-associated protein H-NS and activated by the AraC-like transcriptional regulator ToxT. To elucidate the molecular mechanism by which H-NS controls transcription of the ctxAB operon, H-NS repression and binding were characterized by using a promoter truncation series, gel mobility shift assays, and DNase I footprinting. Promoter regions found to be important for H-NS repression correlated with in vitro binding. Four main H-NS binding regions are present at …

Phylogenetics, Morpohology, And Genomic Evolution In Physarieae (Brassicaceae), Sara Fuentes-Soriano

Dissertations

The mustard family (Brassicaceae) is economically important but the evolution of its morphology is not well understood. I investigate the evolution of morphological and genomic characters and calculate ancestral trait values in the Brassicaceae within a phylogenetic context using the tribe Physarieae as a model system. Physarieae are a unique and diverse group of American mustards characterized by multi-aperturate pollen. Phylogenetic analyses of DNA sequences (chloroplast ndhF and nuclear ITS and LUMINIDEPENDENS) were used to test the monophyly and explore evolutionary relationships of Physarieae. The phylogenetic inferences were used to identify morphological traits to delimit the tribe, to interpret the …

Ua66/5/1 Ogden College Of Science & Engineering Agriculture Student Organizations, Wku Archives

WKU Archives Collection Inventories

Records created by and about Agriculture student organizations including:

• Block & Bridle Club
• Cherry Country Life Club
• WKU Equestrian Team

Gender And Color Specific Differences In Event Related Potentials, Abhishek Trikha

Theses and Dissertations

This project analyzed gender and color-specific differences in event-related potentials (ERPs). Previous studies have shown that males process color differently than females. In a recent study, sex differences were found in ERPs during a visual object recognition task. There were higher EEG amplitudes in females (especially P300) than males. Significant sex and color-specific differences have been found in diseases involving altered dopamine (DA) machinery. Thus, we analyzed differences between ERPs in males vs females during a color task. We also compared the color-specific differences in ERPs between males and females. Males and females participated in EEG recording sessions for 2 …

Development Of A Methodology To Attain High Levels Of Storage Proteins In Cassava Tuberized Roots And Its Implications, Mohammad Abhary

Dissertations

Cassava (Manihot esculenta Crantz.) is a member of the Euphorbiaceae family, cultivated in the tropics and subtropical areas as a staple food crop for more than 700 million people around the world. The starchy storage roots of cassava are rich in calories and deficient in all other nutrients, cassava meals has the lowest of all sources of dietary protein. Severe protein deficiencies and malnutrition problems are common among those who rely on cassava as their daily meal. Although cassava is ranked the fifth most important crop in the world, the research on this crop is very limited. Among the limited …

The Effect Of Passive Stretching And Isometric Contractions On Delayed Onset Muscle Soreness After A Typical Bout Of Exercise, John W. Gibson

Theses and Dissertations

Delayed Onset Muscle Soreness (DOMS) is a common response to activities involving lengthening contractions. Muscle inflammation is associated with DOMS and may play an integral role in protecting a muscle from damage and soreness in response to subsequent bouts of lengthening contractions. Research in animals has shown that prior exposure to passive stretching and isometric contractions of a muscle resulting in muscle inflammation attenuates the muscle inflammatory response following subsequent bouts of lengthening contractions. The purpose of this study was to determine whether passive stretching and isometric contractions in humans would reduce DOMS following a typical bout of resistance exercise. …

The Effects Of Chronic Ampk Activation On Hepatic Triglyceride Accumulation And Glycerol-3-Phosphate Acyltransferaseactivity With High Fat Feeding, Mary E. Curtis

Theses and Dissertations

High fat feeding increases hepatic fat accumulation and is associated with hepatic insulin resistance. AMP Activated Protein Kinase (AMPK) is thought to inhibit lipid synthesis by the acute inhibition of glycerol-3-phosphate acyltransferase (GPAT) activity and transcriptional regulation via SREBP-1c. The purpose of this study was to determine if chronic activation of AMPK prevented an increase in GPAT1 activity in rats fed a high fat diet. Rats were fed a control (C), or a high fat (HF) diet (60% fat) for 6 weeks and injected with saline or a daily AICAR dose of 0.5 mg/g body weight. Chronic AMPK activation by …

Chemical Vapor Deposition Of Silanes And Patterning On Silicon, Feng Zhang

Theses and Dissertations

Self assembled monolayers (SAMs) are widely used for surface modification. Alkylsilane monolayers are one of the most widely deposited and studied SAMs. My work focuses on the preparation, patterning, and application of alkysilane monolayers. 3-aminopropyltriethoxysilane (APTES) is one of the most popular silanes used to make active surfaces for surface modification. To possibly improve the surface physical properties and increase options for processing this material, I prepared and studied a series of amino silane surfaces on silicon/silicon dioxide from APTES and two other related silanes by chemical vapor deposition (CVD). I also explored CVD of 3-mercaptopropyltrimethoxysilane on silicon and quartz. …

The Relationship Between Diet Quality And Body Composition In College Women: A Cross-Sectional Analysis, Annette Elisabeth Perkins

Theses and Dissertations

Objective. Determine the relationship between dietary quality and body weight/composition in college women. Specific emphasis was made regarding adherence to current MyPyramid guidelines, fruit, vegetable and junk food consumption.

Design/Participants. The study used a cross-sectional design. One hundred and sixty three women were recruited to participate in the study. All participants were university students (20.4 ± 1.6 y). Diet intake was measured using the Dietary History Questionnaire (DHQ) and the Healthy Eating Index (HEI) was calculated to assess diet quality. Body fat percentage was assessed using the Bod Pod and BMI was calculated using height and weight measurements. Physical activity …

Promoting Awareness Of Healthy Eating And Physical Activity In Hispanic Women To Reduce Cancer Risk, Donaji Stelzig, Nathalie Folch, Norma Olvera

International Journal of Exercise Science: Conference Proceedings

Latino population is one of the fastest growing minority groups in the United States. As the Latino population continues to increase, chronic diseases such as cancer and obesity will increase. Thus, the development of interventions that promote awareness of healthy eating and an active lifestyle among Latinos are greatly needed. The purpose of this study was to assess the effectiveness of a community-based wellness intervention for Latino women in promoting healthy eating and physical activity. Method: Twenty-four Latino women between the ages 30-65 years participated in a eight-week intervention that included nutrition education focusing on nutrition labeling, selecting healthy food …

Community Support, School Health Programs, And Effective Management On Childhood Obesity, Ling Zhu, Breanca Thomas

International Journal of Exercise Science: Conference Proceedings

No Abstract

Introducing “Symbolic Weightism”: Discourses Of Agency And Morality In Obesity Stigma, Meredith B. Stockton

International Journal of Exercise Science: Conference Proceedings

Title: INTRODUCING “SYMBOLIC WEIGHTISM”: DISCOURSES OF AGENCY AND MORALITY IN OBESITY STIGMA

Author: Meredith B. Stockton, MS

Affiliations: Institute of Social Psychology, London School of Economics and Political Science

Purpose: With the increasing obesity rates in the United States and the United Kingdom, the number of weight-based discrimination cases continues to rise. This study compares obesity stigma to racial stigma, a link first proposed by Crandall (1994), to frame weight-based discrimination as an equally legitimate and severe form of prejudice. Method: Using a social psychological perspective, a thematic and critical discourse analysis was conducted on 170 YouTube video comments and …

Community Based Nutritition Education: A Model For Raising Awareness Of Childhood Obesity, Selina M. Ahmed

International Journal of Exercise Science: Conference Proceedings

Background: Since 2003, Texas Southern University has coordinated conferences to educate Houston’s Third Ward community about strategies to combat childhood obesity. Presenters included faculty and students as speakers and activity coordinators. Participants indicated that these conferences are helpful in increasing awareness of health problems and the availability of community health resources. Purpose: To address the high rate of childhood obesity in Houston’s Third Ward and its costs in terms of health and long-term productivity on that community, faculty at Texas Southern University (TSU) coordinated annual conferences to educate community members about strategies to combat childhood obesity. Methods: Seven conferences intended …

Is There Less Access To Quality Grocery Stores In Minority And Poor Neighborhoods In Houston, Texas?, Chin-Hsing Chen, Lei Zhou, Deborah Banerjee, Monica Slentz

International Journal of Exercise Science: Conference Proceedings

Purpose: There has been an enormous increase in obesity and diet-related diseases in the past decade. Limited access to nutritious foods and easier access to unhealthy foods is believed to be among those at fault. However, access to healthy foods, although studied extensively, does not have a standard measure and is conceptualized in multiple ways by researchers. The relationships between availability of quality grocery store and the socio-demographic profile of the neighborhood are not well understood, particularly in an ethnically and socially diverse city, such as Houston. Our study aims to explore the relationships between distribution of small, medium or …