Life Sciences Commons^™

Minutes, Business Meeting, 35th Annual Meeting, Southeastern Fishes Council, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Comparative Conservation Genetics Of Two Endangered Darters, Percina Rex And Percina Jenkinsi, Anna L. George, David A. Neely, Richard L. Mayden

Southeastern Fishes Council Proceedings

No abstract provided.

Number 52 (December 2010), Southern Fishes Council

Southeastern Fishes Council Proceedings

(December 2010) - Comparative Conservation Genetics of Two Endangered Darters, Percina rex and Percina jenkinsi By Anna L. George, David Neely, and Richard Mayden

Invasion of Gulf Menhaden in the Alabama River By T. Heath Haley, R. Kyle Bolton, and Carol E. Johnston

Southeastern Fishes Council State Reports

Minutes, Business Meeting, 35th Annual Meeting, Southeastern Fishes Council

2009 Treasurer's Report for the Southeastern Fishes Council

English Garden Style: Landscape Design According To The Nineteenth-Century American Seed And Nursery Catalogs, Thomas J. Mickey

Bridgewater Review

No abstract provided.

Applications Of Site-Specific Recombination Systems In Transgene Expression And Marker Gene Removal, Mehmet Aydin Akbudak

Graduate Theses and Dissertations

Site Specific Recombination systems, such as FLP-FRT and Cre-lox, have been successfully used for site-specific gene integration and marker-gene deletion in plant systems. They are very useful tools in the integration of single-copy full-length transgene cassettes into the genome because the transgene integration via conventional methods often generate multi-copy locus. Such complex locus containing direct and inverted repeats of full-length and truncated copies of the transgene cassette generate aberrant RNA resulting in gene silencing. Therefore, for stable gene expression, a single copy transgene locus is preferred. However, even single copy locus sometimes succumbs to gene silencing. Although the mechanism is …

Analysis Of The Response Of Medicago Truncatula Calcium Oxalate Mutants To Abiotic Stress, Wayra Gabriela Navia-Gine

Graduate Theses and Dissertations

Calcium oxalate crystals are found in most plant species. In Medicago truncatula wild-type A17, crystals accumulate in leaves along the secondary veins and the only role attributed to them so far is defense against chewing insects. Calcium oxalate deficient (cod) mutants were isolated in M. truncatula; the cod mutants include cod5, which completely lack crystals in the leaves and cod6 that accumulates fewer and smaller crystals compared to A17. We analyzed gene expression in the cod mutants and A17 using GeneChip® Medicago Genome Arrays and found important differences in transcriptome between the three genotypes. In particular, we found a gene …

A Comparison Of Cumulative-Germination Response Of Cheatgrass (Bromus Tectorum L.) And Five Perennial Bunchgrass Species To Simulated Field-Temperature Regimes, Stuart P. Hardegree, Corey A. Moffet, Bruce A. Roundy, Thomas A. Jones, Stephen J. Novak, Patrick E. Clark, Frederick B. Pierson, Gerald N. Flerchinger

Biology Faculty Publications and Presentations

Cheatgrass (Bromus tectorum L.) has come to dominate millions of hectares of rangeland in the Intermountain western United States. Previous studies have hypothesized that one mechanism conferring a competitive advantage to this species is the ability to germinate rapidly at low temperatures in the fall, winter and spring and, therefore, initiate growth and establishment more rapidly than more desirable perennial bunchgrass species. In this experiment, we developed thermal-germination-response models for multiple seedlots of cheatgrass and five perennial grass species. We conducted sensitivity analysis on potentialcumulative- germination response to a 38-y simulation of field-variable conditions of seedbed temperature and moisture. …

Eighteen Microsatellite Loci Developed From Western Burrowing Owls (Athene Cunicularia Hypugaea), Brant C. Faircloth, Alexandra Title, Kevin Tan, Justin Welty, James R. Belthoff, Patricia Adair Gowaty

Biology Faculty Publications and Presentations

Western burrowing owls (Athene cunicularia hypugaea) are ground-dwelling owls distributed throughout western North America. Because of population declines, this species is considered endangered in Canada, and burrowing owls are listed as a species of conservation concern in states of the western USA. Korfanta et al. (2002) previously presented primers for seven microsatellite loci in burrowing owls. Parentage and relatedness studies require a larger number of markers for accuracy and precision. Here, we developed and characterized 18 additional microsatellite DNA loci, and we tested these loci in 23 individuals. The number of alleles per locus ranged from 2 to …

An Nadph-Dependent Genetic Switch Regulates Plant Infection By The Rice Blast Fungus, Richard A. Wilson, Robert P. Gibson, Cristian Quispe, Jennifer A. Littlechild, Nicholas J. Talbot

Fungal Molecular Plant-Microbe Interactions

To cause rice blast disease, the fungus Magnaporthe oryzae breaches the tough outer cuticle of the rice leaf by using specialized infection structures called appressoria. These cells allow the fungus to invade the host plant and proliferate rapidly within leaf tissue. Here, we show that a unique NADPH-dependent genetic switch regulates plant infection in response to the changing nutritional and redox conditions encountered by the pathogen. The biosynthetic enzyme trehalose-6-phosphate synthase (Tps1) integrates control of glucose-6-phosphate metabolism and nitrogen source utilization by regulating the oxidative pentose phosphate pathway, the generation of NADPH, and the activity of nitrate reductase. We report …

Stride-To-Stride Variability Is Altered During Backward Walking In Anterior Cruciate Ligament Deficient Patients, Franceska Zampeli, Constantina Moraiti Moraiti, Sofia Xergia, Vasilios Tsiaras, Nikolaos Stergiou, Anastasios D. Georgoulis

Journal Articles

Background: Recently backward walking is used by physical therapists to strengthen the hamstring muscles and thus improve the function of the knee joint of anterior cruciate ligament deficient patients. The aim of this study was to examine the stride-to-stride variability of anterior cruciate ligament deficient patients during backward walking. The variation of how a motor behavior emerges in time is best captured by tools derived from nonlinear dynamics, for which the temporal sequence in a series of values is the facet of interest.

Methods: Fifteen patients with unilateral anterior cruciate ligament deficiency and eleven healthy controls walked backwards …

Communication For Mate Selection In Anurans: Dominant Frequency Versus Snout-Vent-Length, Shari Jean Kunert

Department of Environmental Studies: Undergraduate Student Theses

No abstract provided.

Is Orthoptera Abundance And Distribution Across A Small Grassland Area Affected By Plant Biomass, Plant Species Richness, And Plant Quality?, Caitlin Falcone

Department of Environmental Studies: Undergraduate Student Theses

The choice of a specific microhabitat represents a compromise among a number of different factors organisms use to monitor habitat suitability. Grassland vegetation structure can vary widely along environmental gradients over a relatively small area. This vegetation structure can have a large influence on habitat selection by grasshoppers (Orthoptera). However, it is not clear which vegetation characteristics are most important in determining grasshopper abundance. We found that plant biomass, plant species richness, and plant quality all have an effect on grasshopper abundance and distribution. We observe that these affects vary both within and among the two years of data collection. …

The Effect Of Music On Robot-Assisted Laparoscopic Surgical Performance, Ka-Chun Siu, I. H. Suh, Mukul Mukherjee, D. Oleynikov, Nikolaos Stergiou

Journal Articles

Music is often played in the operating room to increase the surgeon’s concentration and to mask noise. It could have a beneficial effect on surgical performance. Ten participants with limited experience with the da Vinci robotic surgical system were recruited to perform two surgical tasks: suture tying and mesh alignment when classical, jazz, hip-hop, and Jamaican music were presented. Kinematics of the instrument tips of the surgical robot and surface electromyography of the subjects were recorded. Results revealed that a significant music effect was found for both tasks with decreased time to task completion (P = .005) and total …

A Comparison Of Interventions For Children With Cerebral Palsy To Improve Sitting Postural Control: A Clinical Trial, Regina T. Harbourne, Sandra L. Willett, Anastasia Kyvelidou, Joan E. Deffeyes, Nikolaos Stergiou

Journal Articles

Background The ability to sit independently is fundamental for function but delayed in infants with cerebral palsy (CP). Studies of interventions directed specifically toward sitting in infants with CP have not been reported.

Objective The purpose of this study was to compare 2 interventions for improving sitting postural control in infants with CP.

Design For this randomized longitudinal study, infants under 2 years of age and at risk for CP were recruited for intervention directed toward sitting independence.

Setting The intervention was conducted at home or at an outpatient facility.

Patients and Intervention Fifteen infants with typical development (mean age …

Obesity, Metabolic Hormone Signaling, And Granulosa Cell Gene Expression, Elizabeth Mack

Department of Animal Science: Dissertations, Theses, and Student Research

It has become increasingly clear that female obesity is associated with a myriad of adverse side effects including abnormal female reproduction due, in part, to amenorrhea and anovulatory infertility. The lethal yellow (LY) mouse possesses a deletion mutation which results in ectopic expression of agouti and adult-onset obesity. Furthermore, LY mice exhibit premature loss of fertility, which has been associated with progressive obesity making the LY mouse line an excellent model to study the effects of obesity-dependent factors on ovarian function. In the current study blood serum and granulosa cells were obtained from LY (Ay/a) and age-matched B6 controls (C57BL/6J) …

Ethanol Byproduct Feeds: Determining Accurate Fiber Content, Nutrient Composition And Variability, Storing With Low-Quality Forages, And Fiber Utilization In Finishing Diets, Crystal D. Buckner

Department of Animal Science: Dissertations, Theses, and Student Research

The growing ethanol industry has produced vast quantities of distillers grains plus solubles (DGS) in the wet (WDGS) and dry forms and Sweet Bran wet corn gluten feed (SB). Previous research has demonstrated that these byproduct feeds result in improved feeding values compared to grass in growing diets and corn in finishing diets, with positive economic returns. Four experiments were conducted to evaluate dry matter determination methods and variability of nutrient composition for WDGS, determining the accurate method for measuring NDF in corn and DGS, compare feeding WDGS mixed with straw as either fresh or ensiled, and evaluate fiber digestibility …

Distillers' Grains With Solubles For Feedlot Cattle: Finishing Performance, Lipid Metabolism, And Ethanol Greenhouse Gas Balance, Virgil R. Bremer

Department of Animal Science: Dissertations, Theses, and Student Research

Previous University of Nebraska feedlot research trials have characterized the feed value of wet distillers grains plus solubles (WDGS). These trials were summarized with meta-analysis methodology and indicated the feed value of WDGS interacts with corn processing type, cattle age (calf-fed or yearling), and inclusion level. Two steer finishing studies and a metabolism study were conducted to understand the impact of different lipid sources in WDGS on WDGS feed value. A biphasic lipid extraction procedure was developed to analyze feed samples from these trials that was more effective than Goldfisch ether extraction at lipid analysis of byproduct feds. These trials …

Tracking Floral Visitation Using Dna Barcodes, Ivan Ray Shoemaker

Theses and Dissertations

Floral visitation resulting in interspecific pollen transfer (IPT) from non-native or invasive plant species can affect the reproductive fitness of native plant species through pollen allelopathy, stigma clogging, stylar clogging, and ovule (seed) discounting. The prevalence of IPT and the importance of pollinators in mediating its impacts, however, remain poorly understood. Although most traditional methods for examining visitor movement are insufficient for determining rates of potential IPT, one promising alternative is the use of DNA barcoding. Because floral visitors eat, collect or unknowingly obtain pollen, nectar and other floral tissues, plant DNA should be recoverable from their bodies, permitting molecular …

Evaluation And Adjustment Of Usda Wasde Cotton Forecasts, David Tysinger

All Theses

The dynamic and volatile nature of agricultural markets causes individuals to rely on forecasts in their decision-making. WASDE cotton forecasts are shown to be especially volatile as cotton is one of the most trade dependent commodities in the world. Therefore, its evaluation requires us to look beyond U.S. WASDE categories and evaluate forecasts for China and the World.
This study will use data from monthly WASDE balance sheets for upland cotton for the U.S, China and World over 1985/1986 through 2008/2009 including unpublished price forecasts. The results of this study will provide information that can be used to improve the …

Chemical Composition And Antioxidant Properties Of Maya Nut (Brosimum Alicastrum), Hatice Kubra Tokpunar

All Theses

ABSTRACT
Maya nut was investigated in term of its moisture, ash content, antioxidant activities, phenolic components by high pressure liquid chromatography-ultraviolet detector (HPLC-UV) and fatty acid composition by gas chromatography-flame ionization detector (GC-FID). The results showed that Maya nut was mainly composed of 55.06% linoleic acid (C18:2 n6), 27.2% palmitic acid (C16:0), 6.26% stearic acid (C18:0), 9.9% linolenic acid (C18:3 n3), and 3.36% Cis-11-Eicosenoic (C20:1). Maya nut possesses a higher content of polyunsaturated fatty acids (PUFAs) (linoleic and linolenic acid), but lower content of monounsaturated fatty acids (MUFAs) than canola oil and olive oil. The antioxidant capacities and total phenolic …

Two-Year Growth And Mortality Of Sub-Canopy Baldcypress (Taxodium Distichum [L.] Rich.) In Artificial Canopy Gaps In A North Carolina Swamp, William Degravelles

All Theses

Baldcypress (Taxodium distichum, [L.] Rich.) -tupelo (Nyssa spp.) swamp forests are a major component of the total area of southeastern U.S. forested wetlands which provide valuable ecosystem services related to water quality, stormwater catchment, and wildlife habitat. Historically, baldcypress has also been an important source of wood products, and clearcutting the principal method of harvesting and regenerating the species. However, anthropogenic alterations to flows of many rivers and the associated flood patterns of alluvial wetlands have prevented the establishment of new baldcypress cohorts in many swamps. Regular, extended growing season flooding could prevent germination and/or establishment of baldcypress seedlings if …

Shelf Life Studies On Processed Peaches, Divija Unhale

All Theses

A series of experiments were conducted on the preservation of fresh peaches using freezing and heat (hot-filled packaging). In the first experiment, the effects of four calcium salts (calcium chloride, calcium lactate, calcium citrate, calcium phosphate) on the texture of processed peach slices were determined. Peach slices were immersed in solutions containing dissolved calcium salts at levels of 0.5, 1 and 2 % for 1 minute then stored at 4 ¡C for 3 days. Treatments with calcium salts were firmer than non calcium treated peach slices at all levels. Calcium lactate at 2 % level was best in terms of …

Femoral Loading Mechanics In Virginia Opossums (Didelphis Virginiana): Torsion And Mediolateral Bending In Mammalian Parasagittal Locomotion, William Gosnell

All Theses

Studies of limb bone loading in terrestrial mammals have typically found anteroposterior bending to be the primary loading regime, with torsion contributing minimally. However, previous studies have focused on large, cursorial eutherian species in which the limbs are held essentially upright. Recent in vivo strain data from the Virginia opossum Didelphis virginiana, a marsupial that uses a crouched rather than upright limb posture, have indicated that its femur experiences moderate torsion during locomotion as well as strong mediolateral bending. The elevated femoral torsion and strong mediolateral bending observed in opossums (compared to other mammals) might result from external forces such …

Measuring Menu Performance By Plate Waste Analysis: An Evaluation Of An Acute Care Facility's Spoken Word Liberalized Menu, Megan Sargeant

All Theses

The Bon Secours St. Francis Downtown Hospital has recently changed their menus to offer meals in which the entrŽes are liberalized when possible. The purpose of this study was to evaluate the performance of a newly introduced menu cycle in an acute-care facility foodservice system featuring a spoken word liberalized menu. Plate waste analysis was done by weighed measurements, using visual measurements when absolutes were observed (all or none). Performance of the menu was assessed with weighed plate waste analysis to determine weight of food consumed, energy consumed, and cost of food wasted. Meal consumption averaged 56% of the meal …

Development Of A Carbohydrate Microarray System And A Microcantilever-Based Biosensor For Detection Of Target Bacteria, Yunyan Cheng

All Theses

The increasing number of disease outbreaks results in a demand for novel pathogen detectors. Carbohydrates serving as receptors for pathogen lectins have become the focus of such research. Two primary sugars, ‡-D-mannose and ‡-L-fucose, as receptors for Escherichia coli and Pseudomonas aeruginosa, respectively, are of great interest to researchers due to their high affinity. These interactions can be studied using carbohydrate microarrays, which are also suitable platforms for detecting bacterial pathogens. In addition, carbohydrates have the potential to act as sensing molecules in microcantilever-based biosensors. The goal of this research was to design a carbohydrate microarray system to study the …

Development, Characterization, And Technical Applications Of An Atlantic Killifish Ahr-2 Specific Monoclonal Antibody (Mab 5b6), Josephine Wojdylo

All Theses

The aryl hydrocarbon receptor (AhR) functions as a ligand-activated transcription factor normally found in the cytoplasmic compartment of cells held by chaperones and immunophilin-like proteins. Ligand binding dissociates the AhR/ligand from chaperone proteins, allowing translocation to the nucleus with subsequent transcription of a suite of responsive genes, most notably Phase I, II, and III drug metabolism genes. Select environmental contaminants such as co-planar PCBs, planar polyaromatic hydrocarbons (PAHs), and halogenated aromatic hydrocarbons (HAHs) are potent AhR agonists, with 2,3,7,8 -tetrachlorodibenzodioxin (TCDD) being one of the most potent. Adverse effects of exposure to these potent environmental AhR ligands include immune suppression, …