Life Sciences Commons^™

Dual Inhibition Of Sodium-Mediated Proton And Calcium Efflux Triggers Non-Apoptotic Cell Death In Malignant Gliomas, William Harley, Candace Floyd, Tamara Dunn, Xiao-Dong Zhang, Tsung-Yu Chen, Manu Hegde, Hasan Palandoken, Michael H. Nantz, Leonardo Leon, K.L. Carraway Iii, Bruce Lyeth, Fredric A. Gorin

Chemistry and Biochemistry

Malignant glioma cells maintain an elevated intracellular pH (pH_i) within hypoxic–ischemic tumormicroenvironments through persistent activation of sodium–proton transport (McLean et al., 2000). Amiloride has been reported to selectively kill human malignant glioma cell lines but not primary astrocytes (Hegde et al., 2004). While amiloride reduces pH_i of malignant gliomas by inhibiting isoform 1 of sodium–proton exchange (NHE1), direct acidification was shown to be cytostatic rather than cytotoxic. At cytotoxic concentrations, amiloride has multiple drug targets including inhibition of NHE1 and sodium–calciumexchange. Amiloride's glioma cytotoxicity can be explained, at least in part, by dual inhibition of NHE1 and …

Reconstructability Analysis Of Epistasis, Martin Zwick

Complex Systems Faculty Publications and Presentations

The literature on epistasis describes various methods to detect epistatic interactions and to classify different types of epistasis. Reconstructability analysis (RA) has recently been used to detect epistasis in genomic data. This paper shows that RA offers a classification of types of epistasis at three levels of resolution (variable-based models without loops, variable-based models with loops, state-based models). These types can be defined by the simplest RA structures that model the data without information loss; a more detailed classification can be defined by the information content of multiple candidate structures. The RA classification can be augmented with structures from related …

The Effects Of Whole Body Vibration On Strength Gains In The Bench Press, The Back Squat, And The Power Clean In Division I Football Players, Kelly S. Poppinga

Theses and Dissertations

The purpose of this study was to determine if whole body vibration effects strength gains in the bench press, the back squat, and the power clean in division 1 football players. Thirty-one NCAA Division 1 male football players volunteered for this study and were randomly assigned to a control group (C=16) or one of two vibration groups (V1=7, V2=8). Subjects followed the training program for eight weeks. A pre-test, mid-test, and post-test one repetition max was measured at 0, 4, and 8 weeks for the bench press, the back squat, and the power clean. A 3x3 factorial ANOVA revealed varied …

Campylobacter Ureolyticus: An Emerging Gastrointestinal Pathogen?, Susan Bullman, Daniel Corcoran, James O'Leary, Brigid Lucey, Deirdre Byrne, Roy D. Sleator

Department of Biological Sciences Publications

A total of 7194 faecal samples collected over a 1-year period from patients presenting with diarrhoea were screened for Campylobacter spp. using EntericBios, a multiplex-PCR system. Of 349 Campylobacter-positive samples, 23.8% were shown to be Campylobacter ureolyticus, using a combination of 16S rRNA gene analysis and highly specific primers targeting the HSP60 gene of this organism. This is, to the best of our knowledge, the first report of C. ureolyticus in the faeces of patients presenting with gastroenteritis and may suggest a role for this organism as an emerging enteric pathogen.

Radiative Forcing Over The Conterminous United States Due To Contemporary Land Cover Use Change And Sensitivity To Snow And Interannual Albedo Variability, Christoper A. Barnes, David P. Roy

GSCE Faculty Publications

Satellite‐derived land cover land use (LCLU), snow and albedo data, and incoming surface solar radiation reanalysis data were used to study the impact of LCLU change from 1973 to 2000 on surface albedo and radiative forcing for 58 ecoregions covering 69% of the conterminous United States. A net positive surface radiative forcing (i.e., warming) of 0.029 Wm−2 due to LCLU albedo change from 1973 to 2000 was estimated. The forcings for individual ecoregions were similar in magnitude to current global forcing estimates, with the most negative forcing (as low as −0.367 Wm−2) due to the transition to forest and the …

Watershed Survey And Pilot Lcc Project, Norman Turgeon, Three Ponds Protective Association

PREP Reports & Publications

This describes two projects accomplished in 2010 by the Three Ponds Protective Association (TPPA) and several partners to identify and reduce soil erosion due to storm water runoff into the Milton Three Ponds

When Curiosity Kills More Than The Cat: The Perils Of Unchecked Scientific Inquiry, Jamie Shannon

Pomona Senior Theses

This work analyzes the ecological, physical, emotional and health impacts of the US nuclear testing done in the Marshall Islands in the mid-20th century.

Spatial Semantics For Better Interoperability And Analysis: Challenges And Experiences In Building Semantically Rich Applications In Web 3.0, Amit P. Sheth

Kno.e.sis Publications

No abstract provided.

Measurements Of Gas‐Phase Inorganic And Organic Acids From Biomass Fires By Negative‐Ion Proton‐Transfer Chemical‐Ionization Mass Spectrometry, P. Veres, James M. Roberts, I. R. Burling, C. Warneke, Joost De Gouw, Robert J. Yokelson

Chemistry and Biochemistry Faculty Publications

[1] Emissions from 34 laboratory biomass fires were investigated at the combustion facility of the U.S. Department of Agriculture Fire Sciences Laboratory in Missoula, Montana. Gas-phase organic and inorganic acids were quantified using negative-ion proton-transfer chemical-ionization mass spectrometry (NI-PT-CIMS), open-path Fourier transform infrared spectroscopy (OP-FTIR), and proton-transfer-reaction mass spectrometry (PTR-MS). NI-PT-CIMS is a novel technique that measures the mass-to-charge ratio (m/z) of ions generated from reactions of acetate (CH₃C(O)O⁻) ions with inorganic and organic acids. The emission ratios for various important reactive acids with respect to CO were determined. Emission ratios for isocyanic acid (HNCO), 1,2 …

Network Science: Using Information Technology To Enable Collaboration, Arden L. Bement Jr.

PPRI Digital Library

No abstract provided.

Simple Sequence Repeat Variation In The Daphnia Pulex Genome, Way Sung, Abraham Tucker, R. Daniel Bergeron, Michael Lynch, W. Kelley Thomas

Molecular, Cellular & Biomedical Sciences

Background: Simple sequence repeats (SSRs) are highly variable features of all genomes. Their rapid evolution makes them useful for tracing the evolutionary history of populations and investigating patterns of selection and mutation across gnomes. The recently sequenced Daphnia pulex genome provides us with a valuable data set to study the mode and tempo of SSR evolution, without the inherent biases that accompany marker selection. Results: Here we catalogue SSR loci in the Daphnia pulex genome with repeated motif sizes of 1-100 nucleotides with a minimum of 3 perfect repeats. We then used whole genome shotgun reads to determine the average …

The Use Of High Distillers' Grains And Nutrient Management In Beef Feedlots, Amy R. Rich

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments were conducted to determine the effect of feeding wet distillers grains (WDGS) at 70% and wheat straw at 25% (DM basis) on finishing steer performance and N mass balance in open feedlot pens. Four treatments were tested as a 2x2 factorial with factors being diet and pen cleaning frequency (monthly or at the end of the feeding period). In both experiments the CON treatment (corn based diet) had greater DMI, ADG, HCW, marbling and fat depth. There was greater N intake and N excretion for both the WINTER and SUMMER experiments on the WDGS. Not all the additional …

Avian Developmental Endocrinology: The Effects And Role Of Yolk Hormones, Sibling Dynamics, And Life-History, Corinne Patricia Kozlowski

Dissertations

Inter-specific variation of the endocrine system is an underlying component for variation in avian life-history. Females deposit hormones into the yolks of their eggs, and embryonic exposure affects nestling phenotype. Hormones are also produced endogenously by developing nestlings, further influencing their behavior and physiology. This dissertation examines the role of the endocrine system in development by focusing on several different avian taxa. Yolk hormone concentrations often vary across the laying order and may mediate hatching asynchrony. These patterns were characterized in two species that lay asynchronously hatching clutches: Eastern screech owls (Megascops asio) and cockatiels (Nymphicus hollandicus). The patterns described …

Ecoweb 1.1: Ecologists' Cooperative Web Bank, Joel E. Cohen

Cohen Laboratory

ECOWeB is a machine-readable database of several hundred food webs compiled from published sources by several ecologists.

A Novel Method To Detect Functional Subgraphs In Biomolecular Networks, Sterling Thomas

Theses and Dissertations

Several biomolecular pathways governing the control of cellular processes have been discovered over the last several years. Additionally, advances resulting from combining these pathways into networks have produced new insights into the complex behaviors observed in cell function assays. Unfortunately, identification of important subnetworks, or “motifs”, in these networks has been slower in development. This study focused on identifying important network motifs and their rate of occurrence in two different biomolecular networks. The two networks evaluated for this study represented both ends of the spectrum of interaction knowledge by comparing a well defined network (apoptosis) with and poorly studied network …

Gene Flow And Dispersal Of The Flatworm, Polycelis Coronata: A Multiscale Analysis, Jeffrey N. Moore

Theses and Dissertations

We determined genetic variance and gene flow across multiple scales (reaches, headwater segments, and catchments) to examine the dispersal ability of the flatworm Polycelis coronata along the Wasatch Mountains of Utah. Multiple models predict patterns of genetic differentiation in stream invertebrates based on dispersal traits and the spatial connectivity of the habitat. The stream hierarchy model predicts genetic differentiation to be low and gene flow to be high between reaches nested in segments, moderate among segments within catchments, and differentiation to be highest and gene flow lowest among catchments, whereas the headwater model predicts the greatest differentiation between headwater segments. …

The Importance Of Sorghum Grain Colour And Hardness, And Their Causes And Measurement, Doreen Mwiita Hikeezi

INTSORMIL Presentations

Discusses sorghum grain color and kernel hardness, assessment methods, and the importance of each to the overall quality of the grain.

Industrial Sorghum Malting: Technologies That Can Be Used And Practical Considerations, Gyebi Duodu

INTSORMIL Presentations

What is malting?

– Germination of grain in moist air under controlled conditions

Objectives for opaque beer brewing

–Mobilization of endogenous enzymes of the grain

–Use of the endogenous enzymes to modify grain constituents for ready solubilisation

–Use of the endogenous enzymes to solubilise starchy adjunct during mashing

Market Development In Support Of Sorghum And Millet Farmers In Tanzania And Zambia, J. Mark Erbaugh, Emmanuel R. Mbiha, Fredy T.M. Kilima, Precious Hamukwala, Gelson Tembo, Donald W. Larson

INTSORMIL Presentations

Problem: Sorghum and millet productivity, production and consumption have been stagnant or declining for 20 years.

Challenge: How to increase the productivity and incomes of sorghum and millet farmers to improve food security and accelerate economic growth.

Solution: Better technology and linking producers to markets are important parts of the solution to the problem.

Objectives

Examine the effects of new production and marketing technology on sorghum and millet producers’ income and productivity in Tanzania and Zambia.

Identify better technologies, enhance market linkages between producers and buyers, improve quality and diversify utilization to ensure food security and increase farm income.

Conclusions: …

Sorghum Malting: Introduction, Objectives, And Scientific Principles, John R.N. Taylor

INTSORMIL Presentations

What is malting? It is limited germination of cereal grains in moist air under controlled conditions.

Why malt sorghum products?

Sorghum malt for home brewing

For brewing opaque beer, lager and stout

For “brewing” malt (malta) type non-alcoholic beverages

To produce malt extract for hot malted beverages,cereal and confectionery flavoring and coloring

For breakfast cereals

For “power flour”–enzyme active flour to “thin” infant porridges

Also explains the science of malting, and the importance of using high-quality grain.

Eagle Lager: A Sorghum Success Story On Many Levels, Ian Mackintosh

INTSORMIL Presentations

In 1999 in Uganda we were faced with a static but highly competitive market.

Needed to grow our market and to do this we needed to make beer cheaper

It was felt that a suitable local ingredient would reduce the cost of manufacture and stimulate local agriculture and the economy as well as provide opportunities for growth and profit for the company.

An extensive base line study of the Ugandan agricultural sector was undertaken to determine what was “out there.”

It was clear that there was enormous potential but little else.

Sorghum seemed the logical starting point because there was …

Improvements In Sorghum Milling Technologies, Martin M. Kebakile

INTSORMIL Presentations

Discusses in some detail various sorghum milling techniques.

Sorghum Malting, Luke Mugode

INTSORMIL Presentations

Malting is defined as the germination of grain in moist air under controlled conditions. Malting occurs in three stages: steeping (soaking), germination, and drying or kilning, then milling can take place. Malting changes the chemistry of the grain.

Virtues Of Sorghum: Utilization And Supply Chain Management, Lloyd W. Rooney

INTSORMIL Presentations

Discusses the consumer demands, value-added supply chain, strategy for value-added products, functionality of sorghums and millets, sorghum food use in Central America, plant breeding and improvement of grain quality, sorghum image, special sorghums as a particularly healthy food item, food utilization, improving sorghum quality, effect of molds, insects, and weathering on grain quality, and mycotoxins.

Breeding Sorghum Cultivars For Processing, Gary Perterson

INTSORMIL Presentations

Discusses the diversity of traits in sorghum and details some aspects of breeding techniques.

Reflections On The Sua-Intsormil Project (2005-2011), Sokoine University Of Agriculture, Joseph J. Mpagalile, Wenceslaus R. Ballegu

INTSORMIL Presentations

Discusses the Sokoine University of Agriculture-INTSORMIL Project that ran from 2005 to 2011. Covers training, research strategy, and stakeholders, and includes recommendations for future projects.

Food Entrepreneurship Curricula, Joseph J. Mpagalile, Wenceslaus R. Ballegu, Jeremia Makindara

INTSORMIL Presentations

SUA Food Entrepreneurship Program Mission Statement: To educate and assist entrepreneurs in developing their sorghum based food manufacturing business. Covers ideas generation, market research, business development, regulations, food safety, brand development, sales and distribution, and with a focus on product development.

Sorghum The Crop Of Yesterday,Today And Tomorrow, S. Simunji

INTSORMIL Presentations

Explains that sorghum will be a crop that survives climate change. Discusses the opportunities and challenges for sorghum crop development in Zambia.

Mycotoxins In Grains – Causes, Prevention And Control, Hester F. Vismer

INTSORMIL Presentations

Discusses mycotoxins, their effect on grain, mitigation considerations, and resulting human toxicology.

Economic Analysis Of Small Scale Sorghum Processing In Dar Es Salaam, Tanzania, Jeremia Makindara, Joseph J. Mpagalile, Wenceslaus Ballegu

INTSORMIL Presentations

Due to small scale food processing initiatives, several entrepreneurs dealing in small scale sorghum processing have emerged in Tanzania and especially in urban centers such as Dar es Salaam. The recent initiative that has contributed to this is the SUA-INTSORMIL Project that started in 2005. However, despite such initiatives being received by many entrepreneurs, very few economic studies have been conducted to assess the benefits obtained by sorghum processing entrepreneurs and the challenges they face are not well explained. This study therefore assessed costs and benefits obtained by small scale sorghum processing entrepreneurs in Dar es Salaam and the challenges …