Life Sciences Commons^™

The Identification Of Cattle Nematode Parasites Resistant To Multiple Classes Of Anthelmintics In A Commercial Cattle Population In The Us, Louis C. Gasbarre, Larry L. Smith, J. Ralph Lichtenfels, Patricia A. Pilitt

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Resistance to modern anthelmintics by ruminant nematode parasites is an increasing problem throughout the world. To date the problem has largely been reported in parasites of small ruminants, but there are increasing reports of such resistance in nematodes recovered from cattle. Until now there have been no published reports of drug resistant parasites from cattle in North America. In 2002 a producer in the upper Midwest who backgrounds young cattle acquired from the southeastern US experienced lower than expected weight gain as well as apparent parasitic gastroenteritis in his cattle during the fall. Fecal sample results supported the suspicion that …

Coccidia (Apicomplexa : Eimeriidae) From The Lagomorph Lepus Tolai In Mongolia, Scott Lyell Gardner, Nathan A. Seggerman, Nyamsuren Batsaikhan, Sumiya Ganzorig, David S. Tinnin, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

In 1999, a single specimen of the Tolai hare, Lepus tolai Pallas, 1778, from the Gobi region of Mongolia was examined and had a new species of eimerian parasite in its intestinal contents. Eimeria gobiensis n. sp. is relatively large; it possesses 2 oocyst walls and a very well-developed oocyst residuum. Oocysts of the new species possess a thick wall with a double layer, a massive 3-layered micropyle, and are ellipsoidal, with average length and width of the oocyst of 38.6 × 24.2 μm, respectively. The range in measurements of these oocysts extends from 27.3 to 49.2 μm in length …

Effects Of Β-Alanine Supplementation And High-Intensity Interval Training On Endurance Performance And Body Composition In Men; A Double-Blind Trial, Abbie E. Smith, Ashley A. Walter, Jennifer L. Graef, Kristina L. Kendall, Jordan R. Moon, Christopher M. Lockwood, David H. Fukuda, Travis W. Beck, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Intermittent bouts of high-intensity exercise result in diminished stores of energy substrates, followed by an accumulation of metabolites, promoting chronic physiological adaptations. In addition, β-alanine has been accepted has an effective physiological hydrogen ion (H⁺) buffer. Concurrent high-intensity interval training (HIIT) and β-alanine supplementation may result in greater adaptations than HIIT alone. The purpose of the current study was to evaluate the effects of combining β-alanine supplementation with high-intensity interval training (HIIT) on endurance performance and aerobic metabolism in recreationally active college-aged men.

Methods: Forty-six men (Age: 22.2 ± 2.7 yrs; Ht: 178.1 ± 7.4 cm; Wt: …

Effects Of Β-Alanine Supplementation And High-Intensity Interval Training On Endurance Performance And Body Composition In Men; A Double-Blind Trial, Abbie E. Smith, Ashley A. Walter, Jennifer L. Graef, Kristina L. Kendall, Jordan R. Moon, Christopher M. Lockwood, David H. Fukuda, Travis W. Beck, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Intermittent bouts of high-intensity exercise result in diminished stores of energy substrates, followed by an accumulation of metabolites, promoting chronic physiological adaptations. In addition, β-alanine has been accepted has an effective physiological hydrogen ion (H⁺) buffer. Concurrent high-intensity interval training (HIIT) and β-alanine supplementation may result in greater adaptations than HIIT alone. The purpose of the current study was to evaluate the effects of combining β-alanine supplementation with high-intensity interval training (HIIT) on endurance performance and aerobic metabolism in recreationally active college-aged men.

Methods: Forty-six men (Age: 22.2 ± 2.7 yrs; Ht: 178.1 ± 7.4 cm; Wt: …

Characterization Of Dictyocaulus Species (Nematoda: Trichostrongyloidea) From Three Species Of Wild Ruminants In Northwestern Spain, Ramon A. Carreno, Natividad Diez-Baños, Maria Del Rosario Hidalgo-Argüello, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Specimens of Dictyocaulus spp. were extracted from the respiratory tracts of 3 ruminant hosts including roe deer (Capreolus capreolus), red deer (Cervus elaphus), and chamois (Rupicapra rupicapra) from wild populations in the province of Leo´n, northwestern Spain. The near-complete nuclear small-subunit ribosomal RNA gene, and 2 regions of the large-subunit ribosomal RNA gene, were amplified by PCR and sequenced. The SSU rDNA gene sequences indicated a high level of similarity between the isolate from C. elaphus and the published sequences for Dictyocaulus eckerti. SSU rDNA gene sequences were identical in the isolates from …

Systematic Position Of Pseudocorynosoma And Andracantha (Acanthocephala, Polymorphidae) Based On Nuclear And Mitochondrial Gene Sequences, Martín García-Varela

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Species of Pseudocorynosoma are North and South American acanthocephalans that use waterfowl as definitive hosts and amphipods as intermediate hosts, whereas species of Andracantha occur in fish-eating birds with a worldwide distribution. Pseudocorynosoma and Andracantha were originally described as Corynosoma (now restricted to endoparasites of marine mammals). Morphologically, Andracantha is distinct from other genera of Polymorphidae in possessing 2 fields of spines on the trunk, whereas Corynosoma and Pseudocorynosoma have a single field. A recent phylogenetic hypothesis based on morphological characters suggested that Andracantha is closely related to Corynosoma, whereas Pseudocorynosoma was of uncertain phylogenetic position within the Polymorphidae. …

Trematoda (Platyhelminthes) Of The Gulf Of Mexico, Robin M. Overstreet, Joshua O. Cook, Richard W. Heard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

First paragraph:

The platyhelminth class Trematoda, consisting of the subclasses Aspidogastrea and Digenea, contains individuals referred to as digeneans, trematodes, or flukes. Aspidogastreans, not digeneans in the strict sense2 number about 80 species in total (Rohde 2005), but adults in the Gulf of Mexico of 4 species have been reported from teleost and chondrichthyan fishes (Hendrix and Overstreet I 977) and one from a turtle (Wharton 1939). Consequently, this chapter deals with trematodes rather than just digeneans, even though all the records but the 5 refer to digeneans. Trematoda is the largest group of platyhelminths, and trematode adults, other than …

Historical Account Of The Two Family-Group Names In Use For The Single Accepted Family Comprising The "Fish Blood Flukes", Stephen A. Bullard, Kirsten Jensen, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The family-group name for the “fish blood flukes” is unstable, with both “Aporocotylidae Odhner, 1912” and “Sanguinicolidae von Graff, 1907” in use for the single family. Although “Sanguinicolidae von Graff, 1907” (or “Graff, 1907”) has been a widely-accepted family-group name for the fish blood flukes subsequent to Yamaguti'’s 1954 and 1958 synoptical publications (“Systema Helminthum”), a critical examination of the relevant literature, much of it published in German during 1900 through 1926, reveals that “Aporocotylidae Odhner, 1912” is the earliest available family-group name for these flukes. The name Aporocotylidae, moreover, was in wide usage by alpha taxonomists before …

Caecincola Longiscens N. Sp. (Digenea: Cryptogonimidae) From The White Crappie, Pomoxis Annularis, In Mississippi, U.S.A., Stephen S. Curran, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new cryptogonimid trematode is described from the white crappie, Pomoxis annularis Rafinesque, 1818, collected from Lake Chotard, an oxbow lake directly connected to the Mississippi River in Issaquena County, and from a small ephemeral oxbow periodically connected with the Pascagoula River in Jackson County, both in Mississippi, U.S.A. Caecincola longiscens n. sp. is characterized by having an elongated body with a length to width ratio of 5.5-7.5:1, tandem testes, an elongated tubular seminal vesicle, and vitelline follicles confined to the hindbody.

Rhipidocotyle Tridecapapillata N. Sp. And Prosorhynchoides Potamoensis N. Sp. (Digenea: Bucephalidae) From Inland Fishes In Mississippi, U.S.A., Stephen S. Curran, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Rhipidocotyle tridecapapillata n. sp. is described from the intestine of the white bass, Morone chrysops (Rafinesque, 1820), from the Luxapalila River in Lowndes County, Mississippi, U.S.A. The new species has 13 papillae that protrude laterally from the anterior rim of the dorsal lobe of the rhynchus, enabling easy differentiation from the only known freshwater North American congeners, Rhipidocotyle papillosa (Woodhead, 1929), which has 15 papillae, and Rhipidocotyle septpapillata Krull, 1934, which has 7 papillae. Prosorhynchoides potamoensis n. sp. is described from the pyloric ceca and intestine of the white crappie, Pomoxis annularis Rafinesque, 1818, from the Pascagoula River in Jackson …

Morphological Deformities As Biomarkers In Fish From Contaminated Rivers In Taiwan, Peter Lin Sun, William E. Hawkins, Robin M. Overstreet, Nancy J. Brown-Peterson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Tilapia (Oreochromis spp.) were collected seasonally from four contaminated rivers in southwestern Taiwan for studies of morphological deformities that could be used as biomarkers of contamination. Morphological deformities found in tilapia were separated into 15 categories. Overall, the prevalence of deformities such as split fins, lower lip extension and gill deformities were significantly related to various water quality parameters, including low DO and high ammonium, lead and zinc concentrations. The persistence of tilapia in polluted waters and the development of a suite of morphological deformities suggest that tilapia can be used as sentinels of non-point source pollution in rivers.

Historical Winter Diets Of Mink (Mustela Vison) In Nebraska, Justin D. Hoffman, Sam Wilson, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Currently there are no published accounts of the specific diets of mink (Mustela vison) in Nebraska. Herein, we present findings of an historic data set on the winter diets of mink in Nebraska. Gastrointestinal (GI) tracts of mink were collected by the Nebraska Game and Parks Commission during the 1946-1947 fur trapping season. The contents of the GI tracts were identified as specifically as possible and percentage of occurrence and percentage of total volume was calculated for each prey item. Mammals and bony fish were the most encountered items. Among mammals, rabbits and muskrats (Ondatra zibethicus) …

Recent Occurrence Of An American Black Bear In Nebraska, Justin D. Hoffman, Sam Wilson, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

We report on a young male, cinnamon-phase American black bear (Ursus americanus) that was collected in northwestern Nebraska on May 12, 2008. This specimen represents the first black bear taken in Nebraska since 1907. Cranial characteristics and mass of the individual indicated a 15–16-month old bear. This bear may have dispersed from the Laramie Mountains in southeastern Wyoming along the North Platte River into western Nebraska. This record adds to the growing number of cases where large carnivores are reinvading previously occupied territory in the Great Plains. These reinvasions potentially have broad ecological implications to local prey populations …

Clinical Relevance Of Sensitization To Lupine In Peanut-Sensitized Adults, K. A. B. M. Peeters, Stef J. Koppelman, A. H. Penninks, A. Lebens, C. A. F. M. Bruijnzeel-Koomen, S. L. Hefle, S. L. Taylor, E. Van Hoffen, A. C. Knulst

Department of Food Science and Technology: Faculty Publications

Background: The use of lupine in food has been increasing during the last decade and allergic reactions to lupine have been reported, especially in peanut-allergic patients. The frequency and the degree of cross-reactivity to other legumes are not known. The aim of the study was to investigate the frequency of sensitization to lupine, and in addition to pea and soy, and its clinical relevance, in peanutsensitized patients. Furthermore, to determine the eliciting dose (ED) for lupine using double-blind placebo-controlled food challenges (DBPCFC).

Methods: Thirty-nine unselected peanut-sensitized patients were evaluated by skin prick tests (SPT) and ImmunoCAP to lupine, pea, and …

Video-Tracking And On-Plant Tests Show Cry1ab Resistance Influences Behavior And Survival Of Neonate Ostrinia Nubilalis Following Exposure To Bt Maize, Jarrad R. Prasifka, Richard L. Hellmich, Andre L. B. Crespo, Blair Siegfried, David W. Onstad

Department of Entomology: Faculty Publications

To examine how resistance to Bacillus thuringiensis (Bt) toxins influences movement and survival of European corn borer (Ostrinia nubilalis [Hübner]) neonates, the responses of Cry1Ab-resistant , -susceptible, and hybrid (F1) larvae were examined using two different techniques. First, using an automated videotracking system, aspects of O. nubilalis movement were quantified in the presence of artificial diet incorporating 50% non-Bt or insect-resistant Cry1Ab maize tissue. Second, O. nubilalis dispersal and survival were measured 48–72 h after hatching on a Cry1Ab maize plant surrounded by two non-Bt maize plants. Video tracking indicated the presence of Cry1Ab tissue increased the total distance …

Pyramidobacter Piscolens Gen. Nov., Sp. Nov., A Member Of The Phylum ‘Synergistetes’ Isolated From The Human Oral Cavity, Julia Downes, Sonia R. Vartoukian, Floyd E. Dewhirst, Jacques Izard, Tsute Chen, Wen-Han Yu, Iain C. Sutcliffe, William G. Wade

Department of Food Science and Technology: Faculty Publications

Four strains of anaerobic, gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group distinct from any species with validly published names. 16S rRNA and 23S rRNA gene sequence analyses and DNA–DNA reassociation data revealed that the strains constituted a novel group within the phylum ‘Synergistetes’ and were most closely related to Jonquetella anthropi. Two libraries of randomly cloned DNA were prepared from strain W5455T and were sequenced to provide a genome survey as a resource for metagenomic studies. A new genus …

Remarks: Neuroscience, Gender, And The Law, Stacey A. Tovino

Faculty Articles

These remarks, delivered at the Neuroscience, Law, and Government Symposium held at the University of Akron School of Law in 2009, explore how stakeholders are using advances in the neuroscience of three gender-specific and gender-prevalent conditions (the postpartum mood disorders, premenstrual dysphoric disorder, and eating disorders) to secure health care benefits under group health plans and individual health insurance policies and to push for the inclusion of these conditions in mental health parity legislation.

Genetic Diversity Of Wilt And Root Rot Pathogens Of Chickpea, As Assessed By Rapd And Issr, Harun Bayraktar, F. Sara Dolar

Turkish Journal of Agriculture and Forestry

Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice's coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates. The isolates that were not easily separable using morphological characteristics were classified again according to their branching in dendrograms. A partial correlation was observed between cluster analysis and geographic origin of Fusarium solani isolates. Additionally, …

Selection For Test Weight And Kernel Weight In High Yielding Wheat Using A Safety-First Index, Emre İlker, Meti̇n Altinbaş, Muzaffer Tosun

Turkish Journal of Agriculture and Forestry

Five breeding lines and 4 cultivars were grown in replicated trials at 1 location in 1999-2000 and 2 locations in 2000-2001 cropping seasons in İzmir in order to determine the usefulness of a safety-first selection index in making selection among high yielding wheat genotypes for test weight and thousand-kernel weight. Genotype × environment interactions were significant for these 2 quality traits. The regression coefficient (b_i) of genotypes, variance (Si_i^2) of a genotype across environments, and a safety-first index (I_i) were calculated to evaluate the stability of genotypes for test weight and thousand-kernel weights. Wheat genotypes with lower bi values and …

The Effects Of Four Organic Soil Conditioners On Aggregate Stability, Pore Size Distribution, And Respiration Activity In A Sandy Loam Soil, Shokrollah Asghari, Mohammad Reza Neyshabouri, Fariborz Abbasi, Nasser Aliasgharzad, Shahin Oustan

Turkish Journal of Agriculture and Forestry

In coarse-textured soils, low water and nutrient holding capacity due to the high number of macropores limits crop growth. To minimize these limitations the application of various soil conditioners (SCs) has been extensively used in recent decades. The objective of this study was to investigate the influences of anionic polyacrylamide (PAM), cattle manure (M), vermicompost (VC), and biological sludge (BS) as organic SCs on mean weight diameter (MWD) of aggregates, water aggregate stability (WAS), pore size distribution (PRSD), and respiration activity (RA) of a sandy loam soil. PAM (0.25 and 0.5 g kg^{-1} of air-dried soil), M (12.5 and 25 …

Evaluation Of A Low-Cost Gps Receiver For Precision Agriculture Use In Adana Province Of Turkey, Muharrem Keski̇n, Sai̇t Muharrem Say, Serap Görücü Keski̇n

Turkish Journal of Agriculture and Forestry

The most vital factor in the application of precision agriculture technology is the cost of the required high-technology equipment. The equipment cost is the major obstacle in adopting the precision agriculture. GPS receiver is one of the most essential tools with high initial costs in this technology. The aim of this study was to evaluate a low-cost GPS receiver in 3 different tests including static, dynamic circular area, and dynamic straight line tests. It was observed that the tested low-cost GPS receiver yielded a deviation of less than 1.50 m, 1.60 m, and 1.48 m in static, circular area, and …

Development Of A Computerized Measurement System For In-Row Seed Spacing Accuracy, Okan Önal, İsmet Önal

Turkish Journal of Agriculture and Forestry

The aim of this study was to develop a computerized measurement system (CMS) combined with a sticky belt test stand for examining in-row seed spacing distribution accuracy. For this purpose 8 performance parameters were selected for precision seeding: mean seed spacing, the standard deviation, the multiples index, the miss index, the quality of feed index, the precision, the population index, and the coefficient of precision (CP-3). The CMS hardware consisted of a high precision optical mouse coupled with a laser pointer and a notebook computer. The use of optical laser technology is a new method for the determination of seed …

Soybean Seed Yield Performances Under Different Cultural Practices, Mehmet Si̇nci̇k, Abdurrahi̇m Tanju Göksoy, Zeki̇ Meti̇n Turan

Turkish Journal of Agriculture and Forestry

This study was carried out to investigate the yield response of 2 soybean (Glycine max (L.) Merrill) cultivars to different planting dates and plant populations and to examine the relationships of seed yield with leaf area index (LAI) and total dry matter (TDM) during the vegetative and reproductive periods for planting dates, plant populations, and cultivars. Two soybean cultivars, A-3127 (early maturity group, MG-III) and 1530 (late maturity group, MG-IV), were planted in 2 planting dates; mid-April and mid-May during 2005 and 2006 at high (660,000 plant ha^{-1}) and low (330,000 plant ha^{-1}) plant populations. Plantings in mid-April increased seed …

Investigations On The Pollen Morphology Of Some Fruit Species, Yasemi̇n Evrenosoğlu, Adalet Misirli

Turkish Journal of Agriculture and Forestry

Morphological characters are used to identify plant material. Pollen morphology is an important parameter. This is of great importance in detailed investigations by scanning electron microscope (SEM). In order to develop a plant material identification tool, this research was carried out to determine the pollen morphology of some fruit species using SEM on apple, pear, quince, apricot, plum, peach, almond, chestnut, walnut, pomegranate, and persimmon cultivars. The surface features, as well as length, width, and P/E ratio of pollen were observed. Pollen length and width changed with investigated species and cultivars; the longest and widest pollen grains were detected in …

Fumigant Toxicity Of Plant Essential Oils And Selected Monoterpenoid Components Against The Adult German Cockroach, Blattella Germanica (L.) (Dictyoptera: Blattellidae), Hasan Tunaz, Mehmet Kubi̇lay Er, Ali̇ Arda Işikber

Turkish Journal of Agriculture and Forestry

The fumigant toxicity of various essential oils and selected monoterpenoid components were tested against the adult German cockroach, Blattella germanica (L.) (Dictyoptera: Blattellidae). Responses varied according to plant material, concentration, and exposure time. High insecticidal activity against adult B. germanica specimens was achieved with allyl isothiocyanate (component of horseradish) and the essential oil of Allium sativum (L.) (Liliales: Liliaceae) at the concentration of 2.5 and 5 \mul l^{-1} of air, respectively, while the essential oils of Thymus vulgaris (L.) (Lamiales: Lamiaceae), Oregano dubium (L.) (Lamiales: Lamiaceae), Allium cepa (L.) (Liliales: Liliaceae), and Rosemarinus officinalis (L.) (Lamiales: Lamiaceae), and the monoterpenoid …

Determination Of The Head Losses In Metal Body Disc Filters Used In Drip Irrigation Systems, Vedat Demi̇r, Hüseyi̇n Yürdem, Arzu Yazgi, Adnan Deği̇rmenci̇oğlu

Turkish Journal of Agriculture and Forestry

The objective of this study was to determine the head losses in several type metal body disc filters manufactured by domestic companies. In order to meet the objective, three different filter designs (L, LT and Y type), each at four different inlet and outlet pipe diameters, were used to measure the head losses at different flow rates in the laboratory. A total of sixteen filters manufactured by three domestic companies (A, B and C) and two different disc elements (linear and curly) were taken into consideration in this study. A comparison of the different filter designs in terms of measured …

Agronomic Trait Assessment And Selection For Number Of Capsules In Determinate X Indeterminate Crosses Of Sesame, M. İlhan Çağirgan, Selçuk Özerden, M. Onur Özbaş

Turkish Journal of Agriculture and Forestry

Despite the progress, improved determinate (dt/dt) lines of sesame are still low yielding. Further improvement may be achieved by crossing locally adapted indeterminate cultivars with desirable determinates from a broad-based composite population. In order to test this hypothesis, 43 F_2 base populations from these kinds of crosses were generated and a distinct variability among populations was observed for seed yield and other traits. A 20% positive selection for number of capsules as the most yield-related component was applied to the pairs of determinate and indeterminate segregating in each population. Although seed yield per plant, number of capsules per plant and …

Reproduction And Survival Of Overwintered And F_1 Generation Of Two Egg Parasitoids Of Sunn Pest, Eurygaster Integriceps Put. (Heteroptera: Scutelleridae), Şener Tarla, Serpi̇l Kornoşor

Turkish Journal of Agriculture and Forestry

The differences in the reproductive biology of overwintered (OW) and F_1 generation of Trissolcus semistriatus Nees and T. festivae Victorov (Hymenoptera: Scelionidae) were investigated under laboratory conditions. The experiments were conducted to evaluate oviposition period, lifetime fecundity, female progeny, sex ratio, and longevity of OW and F_1 generation with the two species as factors. The results of this study showed that the oviposition period of F_1 generation was longer than that of OW females for both of the two parasitoid species. With the exception of F_1 generation of T. festivae, the highest daily average progeny production of females was recorded …

Orthotomicus Tridentatus Eggers: Distribution And Biology In Cedar Forests Of Turkey, Mustafa Avci, Oğuzhan Sarikaya

Turkish Journal of Agriculture and Forestry

Orthotomicus tridentatus Eggers, 1921 (Col.: Curculionidae, Scolytinae) is the most important forest pest of cedar stands in Turkey. Yet, very little is known about its distribution and biology. Therefore, the distribution and biological characteristics of O. tridentatus were studied in the Taurus Mountains in southern Turkey between 2006 and 2008. It was determined to be a species common to all cedar forests. The length of adults was between 2.5 and 3.9 mm and the main galleries were 'S' shaped. O. tridentatus was determined to be a monophagus species for Cedrus libani A. Rich. and as endemic to Turkey. It was …

Isolation And Functional Characterization Of Two Rapeseed Genes Orthologous To Arabidopsis Thaliana Phenylalanine Ammonia-Lyase 2, Yu Ni, Yan-Zhen Dong, Guan-Rong Li, Bo Lei, You-Rong Chai, Jia-Na Li

Turkish Journal of Agriculture and Forestry

Two rapeseed (Brassica napus L.) phenylalanine ammonia-lyase 2 genes (BnPAL2-1 and BnPAL2-2) were isolated. The full-length cDNAs of BnPAL2-1 and BnPAL2-2 are 2422 bp and 2421 bp, with corresponding genomic sequences of 2735 bp and 2742 bp, respectively. Clues from NCBI BLAST, multi-alignment, the phylogenetic tree, conserved domains, preferential amino acids, and tertiary structures all indicated that they are orthologs of Arabidopsis thaliana PAL2 (AtPAL2). The purified His-tagged BnPAL2-1 and BnPAL2-2 proteins, expressed in Escherichia coli, were both active for PAL catabolism, but BnPAL2-2 was much more active than BnPAL2-1. Southern blot analysis showed that the BnPAL2 subfamily might contain …