Life Sciences Commons^™

Characterization Of Membrane Potentials In Vascular Smooth Muscle Of Hagfish, Lamprey And Trout, Prentiss Jones Jr.

Dissertations

The objective of this study was to characterize membrane potentials in systemic arteries of Pacific hagfish, Eptatretus stouti, Rainbow trout, Onchorhyncus mykiss, and Sea lamprey Petromyzon marinus. Previous studies have characterized membrane potentials in piscine tissue. However, these studies utilized non-vascular tissues such as cardiac muscle and skeletal muscle (69, 161). Characterization of membrane potentials in fish vasculature is without precedent in the literature. The hypothesis of this study was that membrane potentials in fish vascular smooth muscle differ little between species when comparable vasculature is evaluated.

Effect Of Ozone And Calcium Lactate Treaments On Browning And Textured Properties Of Fresh-Cut Lettuce, Daniel Rico, Ana Belen Martin-Diana, Gary Henehan, Jesus Maria Frias, Catherine Barry-Ryan

Articles

The effects of three treatments, 1 mg L⁻¹ ozone at 18–20 °C, 15 g L⁻¹ calcium lactate (CLac) at 50 °C and a combination thereof, were compared on fresh-cut lettuce over 10 days of refrigerated storage. Respiration rate, browning and texture were examined as main quality indicators. The use of ozone produced a significantly (P < 0.05) higher oxygen decline than the use of CLac (from day 3 to day 10). At the end of storage, CLac (alone or combined with ozone) samples had higher oxygen content (∼9%) than ozone samples (∼6%). Enzymatic activity decreased significantly (P < 0.05) in ozone samples. Polyphenol oxidase activity in fresh-cut lettuce treated with ozone (alone or combined with CLac) showed lower values on day 1 (−1) and at the end of storage (g⁻¹) than CLac samples (4000–4800 units g⁻¹). Ozone also reduced peroxidase activity to ∼300 units g⁻¹ after treatment. Finally, pectin methylesterase activity was also reduced with ozone, showing a negative effect on textural properties. Data suggested that …

Xenobiotic Response In Drosophila Melanogaster: Sex Dependence Of P450 And Gst Gene Induction, Gaelle Le Goff, Frederique Hilliou, Blair Siegfried, Sam Boundy, Eric Wajnberg, Luc Sofer, Pascaline Audant, Richard H. Ffrench-Constant, Rene Feyereisen

Department of Entomology: Faculty Publications

The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real-time RT-PCR in adults of both sexes. Only a small subset of the 86 CYP genes was significantly induced by the xenobiotics. Eleven CYP genes and three glutathione S-transferases (GST) genes were significantly induced by phenobarbital, seven CYP and one GST gene were induced by atrazine. Cyp6d5, Cyp6w1, Cyp12d1 and the ecdysone-inducible Cyp6a2 were induced by both chemicals. The constitutive expression of several of the inducible …

A 100 Meter Training Walk Using A Speed Trainers™ Device Does Not Strengthen The Hip Flexors In Collegiate Sprinters, Jayme Donald Jasper

All-Inclusive List of Electronic Theses and Dissertations

Context: Many types of resistance training programs have been implemented in order to enhance sprint performance, however, most programs have overlooked the importance of the hip flexor muscles. Objective: To determine if a training protocol using a Speed Trainers ™ device will increase hip flexion strength, and if that increase in strength has an effect on competition sprint times. Design: A 2 X 2 pre-test post-test design with treatment (hip flexor strength training and sham group) and time (pre-training and posttraining) as the independent variables. Setting: The strengthening program took place at the Indiana State University outdoor track. Competition sprint …

The Effect Of Prophylactic Ankle Bracing On Star Excursion Balance Measures In Uninjured Subjects, Lisa Marie Hardy

All-Inclusive List of Electronic Theses and Dissertations

Context: Reports of bracing on whole body bilateral movements and single directional motions are unclear about whether or not the performance of uninjured active people is affected. Objective: To isolate the braced limb, while performing multidirectional tasks, to gain a better understanding of the effects of bracing on performance. Design: A 1 X 3 repeated measures design with the bracing condition (unbraced, lace-up, and semi-rigid) as the independent variable. Setting: Laboratory. Patients or Other Participants: Thirtysix uninjured, physically-active, volunteers (M: 18, F: 18, age: 23 .6±2. 7y, ht: 173.8±9.3cm, mass: 74.4±12.7kg, reach leg length: 91.9±5.lcm). Main Outcome Measure(s): The average …

The Effect Of Athletic Training Program Course Progression And Implementation Of Course Progression On First-Time Success Rate On The Board Of Certification Examination, Matthea S. Hungerford

All-Inclusive List of Electronic Theses and Dissertations

Context: For optimal preparation for the Board of Certification (BoC) examination, the environment in which a student learns should be designed to disseminate the appropriate knowledge in a sequenced, timely manner. Objective: To determine if there is a relationship between progression of courses (prevention and care, first evaluation course, rehabilitation, modalities, organization and administration) and their respective timing on first-time pass rate of the written and overall BoC examination. Design: Multiple Pearson correlational analyses. Setting: Web-based survey. Patients or Other Participants: Program directors from 31 Commission on Accreditation of Allied Health Education Programs (CAAHEP) accredited entry-level undergraduate athletic training education …

Arsenic Exposure Is Associated With Decreased Dna Repair In Vitro And In Individuals Exposed To Drinking Water Arsenic, Angeline S. Andrew, Jefferey L. Burgess, Maria M. Meza, Eugene Demidenko, Mary G. Waugh, Joshua W. Hamilton, Margaret R. Karagas

Dartmouth Scholarship

The mechanism(s) by which arsenic exposure contributes to human cancer risk is unknown; however, several indirect cocarcinogenesis mechanisms have been proposed. Many studies support the role of As in altering one or more DNA repair processes. In the present study we used individual-level exposure data and biologic samples to investigate the effects of As exposure on nucleotide excision repair in two study populations, focusing on the excision repair cross-complement 1 (ERCC1) component. We measured drinking water, urinary, or toenail As levels and obtained cryopreserved lymphocytes of a subset of individuals enrolled in epidemiologic studies in New Hampshire (USA) and Sonora …

The Influence Of Low Humidity, Pseudacteon Flies, And Competition By Solenopsis Xyloni On Solenopsis Invicta, Ricardo A. Ramirez, D C. Thompson, M D. Remmenga

Biology Faculty Publications

Red imported fire ant, Solenopsis invicta Buren, is a serious pest in the southeastern United States. It has caused economic losses in agricultural products, stings can cause anaphylactic shock, and it has reduced biodiversity. S. invicta has displaced native ant species throughout its range, presumably by competitive exclusion. In 1998, S. invicta populations were confirmed in southern New Mexico, resulting in a quarantine of Doña Ana County. Although large populations of the native southern fire ant, S. xyloniMcCook, are common in the Mesilla Valley, only two small populations of S. invicta have been found since the initial quarantine. It is …

Genetic Considerations For The Conservation And Management Of Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) In Yellowstone National Park, David J. Janetski

Theses and Dissertations

A key component to conservation is an accurate understanding of genetic subdivision within a species. Despite their ecological and economic importance, relatively little is understood about the genetic structuring of Yellowstone cutthroat trout in Yellowstone National Park. Here, we use traditional (Fst, Rst, Nm, and AMOVA) and modern (Bayesian assignment tests, coalescent theory, and nested clade analysis) analytical approaches to describe the population genetic subdivision of cutthroat trout spawning populations in Yellowstone Lake and to identify genetically distinct population segments throughout Yellowstone National Park. Evidence for restricted gene flow between spawning populations within Yellowstone Lake was detected using nested clade …

Analyses Of Cry1ab Binding In Resistant And Susceptible Strains Of The European Corn Borer, Ostrinia Nubilalis (Hubner) (Lepidoptera: Crambidae), Herbert A.A. Siquiera, Joel Gonzalez-Cabrera, Juan Ferre, Ronald Flannagan

Department of Entomology: Faculty Publications

Cry1Ab toxin binding analysis was performed to determine whether resistance in laboratory-selected Ostrinia nubilalis strains is associated with target site alteration. Brush border membrane vesicles were prepared using dissected midguts from late instars of susceptible and resistant strains (Europe-R and RSTT) of O. nubilalis. Immunoblot analysis indicated that three different proteins bound to Cry1Ab toxin and were recognized by an anticadherin serum. In a comparison of resistant and susceptible strains, reduced Cry1Ab binding was apparent for all three bands corresponding to cadherin-like proteins in the Europe-R strain, while reduced binding was apparent in only one band for the RSTT …

Aspidoderidae From North America, With The Description Of A New Species Of Aspidodera (Nematoda: Heterakoidea), F. Agustin Jimenez-Ruiz, Scott Lyell Gardner, Andrea S. Varela-Stokes

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Aspidodera sogandaresi n. sp. (Heterakoidea: Aspidoderidae) from Dasypus novemcinctus Linnaeus, 1758 is herein described. This nematode occurs in armadillos from as far south as the canal zone of Panama, north through central Mexico, and into the southern United States. Previously identified as Aspidodera fasciata (Schneider, 1866), this new species has blunt projections on the lips and lateral expansions at the distal tips of the spicules, whereas A. fasciata has conspicuous digitiform projections on the lips, and a terminal round expansion at the tips of the spicules. Other species of the family present in North America include Aspidodera binansata Railliet and …

Isolation Of An Active Lv1 Gene From Cattle Indicates That Tripartite Motif Protein-Mediated Innate Immunity To Retroviral Infection Is Widespread Among Mammals, Laura M.J. Ylinen, Zuzana Keckesova, Benjamin L.J. Webb, Robert J.M. Gifford, Timothy P.L. Smith, Greg J. Towers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Lv1/TRIM5α (tripartite motif 5α) has recently emerged as an important factor influencing species-specific permissivity to retroviral infection in a range of primates, including humans. Old World monkey TRIM5α blocks human immunodeficiency virus type 1 (HIV-1) infectivity, and the human and New World monkey TRIM5α proteins are inactive against HIV-1 but active against divergent murine (N-tropic murine leukemia virus [MLV-N]) and simian (simian immunodeficiency virus from rhesus macaque [SIVmac]) retroviruses, respectively. Here we demonstrate antiviral activity of the first nonprimate TRIM protein, from cattle, active against divergent retroviruses, including HIV-1. The number of closely related human TRIM sequences makes assignment of …

Immunogenicity Of Terephthalate, A Constituent Of Synthetic Polymers, And Its Modulation By Adjuvants, Youssef Aachoui

All-Inclusive List of Electronic Theses and Dissertations

Objective: In this study we have focused on the potential immunotoxicity resulting from exposure to phthalate compounds which are widely present as basic constituents of many synthetic polymers.

Population Models For Threshold-Based Control Of Tetranychus Urticae In Small-Scale Kenyan Tomato Fields And For Evaluating Weather And Host Plant Species Effects, Markus Knapp, Ibrahima Sarr, Gianni Gilioli, Johann Baumgärtner

All Peer-Reviewed Publications

The spatial distribution of motile life stages of the two-spotted spider mite Tetranychus urticae Koch in Kenyan small-scale tomato fields was described by Taylor's power law and an enumerative sampling plan was designed for research purposes. The exponential increase of T. urticae populations during three growing seasons permits the development and use of a simple exponential model for the design of a threshold-based chemical control system. For this purpose, a critical threshold of 440 motile mites per sample unit at the end of the growing season (12 weeks after transplanting) was translated into a proportion of 0.83 infested units in …

Virion-Associated Restriction Endonucleases Of Chloroviruses, Irina V. Agarkova, David Dunigan, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Chloroviruses are large, double-stranded-DNA, plaque-forming viruses that infect certain eukaryotic chlorella- like green algae. The prototype of the genus is Paramecium bursaria chlorella virus 1 (PBCV-1). Chlorovirus genomes contain various amounts of methylated nucleotides due to virus-encoded DNA methyltransferases (MTases); about 25% of the MTases are associated with companion DNA site-specific (restriction) endonucleases (REases). These enzymes constitute virally encoded restriction-modification (R/M) systems. Although several of the chlorovirus R/M systems are characterized, their biological functions are unknown. The PBCV-1 proteome reveals that two virus-encoded REases, but not their companion MTases, are virion associated, suggesting that viral REases might help degrade the …

Notes On Invasive Plants In The Rio Grande Delta Of Cameron County, Texas., Robert I. Lonard, Frank W. Judd

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

No abstract provided.

The Acute Effects Of Nut Ingestion On Plasma Levels Of Polyphenol Compounds, Biomarkers Of Antioxidant Status, And Lipid Peroxidation, Setareh Torabian Esfahani

Loma Linda University Electronic Theses, Dissertations & Projects

Nuts are rich sources of polyphenolic compounds and nutrients, which have antioxidant capacity. To assess the acute effect of treatment diet (75% of energy from nuts (walnut or almond) and control diet (habitual diet with no nut consumption) in healthy subjects on plasma total phenol content, antioxidant capacity and plasma lipid peroxidation. Thirteen subjects participated in the crossover design study. After an overnight fast, walnut, almond and a control diet, in form of smoothies, were consumed by the study subjects. Each subject participated on three occasions one week apart, consuming one of the smoothies each time. Blood samples were obtained …

Stream Buffer Characterization Study, David G. Justice, Fay A. Rubin

PREP Reports & Publications

The Complex Systems Research Center at the University of New Hampshire conducted a characterization of 2nd order and higher streams within the Piscataqua/Coastal Basin of New Hampshire. GIS and remote sensing data archived in the NH GRANIT database were used to map a suite of anthropogenic factors, including land use, impervious surface coverage, and transportation infrastructure, within standard buffers around each stream segment. These factors were then analyzed to produce a categorical indicator representing the status of each stream.

Mbet3p Is Required For Homotypic Copii Vesicle Tethering In Mammalian Cells, Sidney Yu, Ayano Satoh, Marc Pypaert, Karl Mullen, Jesse C. Hay, Susan Ferro-Novick

Biological Sciences Faculty Publications

TRAPPI is a large complex that mediates the tethering of COPII vesicles to the Golgi (heterotypic tethering) in the yeast Saccharomyces cerevisiae. In mammalian cells, COPII vesicles derived from the transitional endoplasmic reticulum (tER) do not tether directly to the Golgi, instead, they appear to tether to each other ( homotypic tethering) to form vesicular tubular clusters (VTCs). We show that mammalian Bet3p (mBet3p), which is the most highly conserved TRAPP subunit, resides on the tER and adjacent VTCs. The inactivation of mBet3p results in the accumulation of cargo in membranes that colocalize with the COPII coat. Furthermore, using an …

College Of Agricultural Sciences And Natural Resources: 13th Annual Report August 1, 2005-July 31, 2006

College of Agricultural Sciences and Natural Resources (CASNR): Annual Reports

Contents
Introduction 3 • • Dedication 7 • Administration and Staff 8 • Academic Unit Administrators 8 • CASNR Standing Committees 9 • Faculty Advisory Council 9 • Scholarship and Graduation with Distinction Committee 12 • Curriculum Committee12 • Teaching Faculty 13 • Faculty Meetings 13 • New Faculty or Faculty with New Appointments 13 • Retirements 13 • Faculty Awards 14 • Departmental News and Events 16 • Agricultural Economics17 • Agricultural Leadership, Education and Communication 19 • Agronomy and Horticulture 21 • Animal Science 28 • Biochemistry 40 • Biological Systems Engineering 41 • Entomology 47 • Food …

Genetics And Inheritance Of Seed Dormancy Inflicted By Seed- Coat Factors In Rice (Oryza Sativa L.), Z. L. Kanyeka

Tanzania Journal of Science

The study was undertaken to investigate the genetic mode of inheritance of dormancy imposed by the hull (seed coat) in rice seeds. Freshly harvested seeds of parents, F1 and F2 populations of a cross between a dormant cultivar Kisegese and non-dormant strain K2004 were used. Germination test of the de-hulled (without seed coat) and un-dehulled (whole or with seed coat) seeds were conducted at 10 days intervals starting at 15 days after harvesting (15DAH) for 65 days. The seeds were sown in petri dishes. Numbers of germinated and un-germinated seeds were recorded at 7 days after sowing. Germination of seeds …

Physico-Chemical And Grain Cooking Characteristics Of Selected Rice Cultivars Grown In Morogoro, N.B. Shayo, P. Mamiro, C. N. M. Nyaruhucha, T. Mamboleo

Tanzania Journal of Science

A study was carried out at Sokoine University of Agriculture to characterize the local rice varieties grown in Morogoro Region basing on their physical-chemical characteristics and cooking quality parameters. Five cultivars of rice were analyzed and these included Kaling'anaula, Kihogo Red, Salama M17, Supa and Salama. The samples were collected from Crop museum, SUA. The chemical analysis was done to determine the proximate composition, gelatinization temperature and gel consistency as main determinants of cooking quality, sensory evaluation tests was done as determinants for consumer acceptance. The proximate composition results indicated protein content values between 7.94 and 9.46%, ash content between …

Pathogenecity Of Two Strains Of Rice Yellow Mottle Virus On Aromatic Rice Cultivars, Z. L. Kanyeka

Tanzania Journal of Science

Development of rice cultivars with resistance to rice yellow mottle virus (RYMV) requires characterization of pathogenecity of existing strains against susceptible cultivars. The pathogenecity of two RYMV strains; S4 and S“New” identified in Tanzania were assessed against seven local and introduced rice genotypes including cultivars Zambia, ITA173 and TXD 88. The two virus strains showed similar effects on the rice cultivar Zambia killing all the plants at 25d after inoculation. The strains significantly reduced the plant height, panicle length, and number of tillers per pot and total dry matter of all the two cultivars. In another trial, the pathogenecity of …

Occurrence Of Metacercariae Of Diplostomum And Tylodelphys Species (Diplostomidae) In Clarias Species (Clariidae) Fishes From Lake Victoria, M. J. Musiba, G. Nkwengulila

Tanzania Journal of Science

The localisation and abundance of the metacercariae of Diplostomum and Tylodelphys species among the clariids of Lake Victoria were investigated between April and December 2001. Four species viz. Clarias gariepinus (271), C. alluaudi (65), C. werneri (50) and C. liocephalus (47) were examined, but only C. gariepinus was infected in the brain case and along the nasal cavity. Prevalence of metacercariae was high (>70%). Prevalence and mean intensity were similar between male and female fish. Mean intensity increased with fish size and declined in larger fish for D. mashonense; but declined with the increase in fish size for Tylodelphys …

Diversity And Abundance Of Small Mammals In The Serengeti Kopjes, Tanzania, C. D. Timbuka, J. D. Kabigumila

Tanzania Journal of Science

Diversity and abundance of small mammals in the Serengeti kopjes were studied between November 2000 and February 2002. From 22,500 trap-nights and 3,300 bucket-nights, 4,903 small mammals in 18 species, 14 genera, 6 families and 3 orders were recorded. Muridae was the most dominant family with nine species. The highest number of species was recorded in the Maasai (14), Mbuzi Mawe (13) and Seronera (12) kopjes. The least number of species recorded in the Simba, Barafu and Wogakuria kopjes, with eight species each. Species diversity was highest in the Maasai kopjes (H'=2.337) and lowest in the Wogakuria kopjes (H'=1.794), with …

Interview With Peter Hayes, Hyla Woods, 2006 (Audio), Peter Hayes

All Sustainability History Project Oral Histories

Interview of Peter Hayes by Glen Esler on July 28th, 2006.

The interview index is available for download.

Oyster Reef Restoration Project For The City Of Dover, Grizzle, Raymond E. Grizzle, Jennifer Greene, Holly Abeels

PREP Reports & Publications

This project was conducted as a contract between the City of Dover and the University of New Hampshire, with additional funding supplied by the New Hampshire Estuaries Project. The overall goal was to restore as much bottom area as possible (with available funds) of formerly productive oyster bottom in two areas, the Bellamy River and Pomeroy Cove (Piscataqua River). The restored areas were intended as a contribution to the long-term NHEP goal of restoring 20 acres of oyster bottom by 2010 (Trowbridge 2003). Five objectives were addressed: (1) site surveys, map production, and final restoration protocol development; (2) remote setting …

Circadian Rhythmicity By Autocatalysis, Arun Mehra, Christian I. Hong, Mi Shi, Jennifer J. Loros, Jay C. Dunlap, Peter Ruoff

Dartmouth Scholarship

The temperature compensated in vitro oscillation of cyanobacterial KaiC phosphorylation, the first example of a thermodynamically closed system showing circadian rhythmicity, only involves the three Kai proteins (KaiA, KaiB, and KaiC) and ATP. In this paper, we describe a model in which the KaiA- and KaiB-assisted autocatalytic phosphorylation and dephosphorylation of KaiC are the source for circadian rhythmicity. This model, based upon autocatalysis instead of transcription-translation negative feedback, shows temperature-compensated circadian limit-cycle oscillations with KaiC phosphorylation profiles and has period lengths and rate constant values that are consistent with experimental observations.

Smokejumper Obituary: Norrod, Stephen (Myc 1978), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Acer Saccharum Marshall, Gordon C. Tucker

Specimens by Name

No abstract provided.