Life Sciences Commons^™

Calorie Restriction Enhances Longevity Without Reducing Lifetime Fecundity Or Glucose Titers In Female Lubber Grasshoppers, Sean M. Wells, Iris Cynthia Allen, Erin Fuller, Stephen Melnyk, John Quattrochi

All Volumes (2001-2008)

Calorie restriction, under eating while avoiding malnutrition, enhances longevity in many organisms, in part by delaying fecundity or lowering blood glucose. Calorie restriction begun at middle-age can also enhance longevity. We tested four diets on longevity of female lubber grasshoppers: ad libitum (free access to food), calorie restriction (60% or 71% of ad libitum), and delayed calorie restriction (60% after day 50). Constant calorie restriction increased longevity in grasshoppers by at least 66%. These diets lowered body mass, but it did not reduce lifetime fecundity or chronically lower blood glucose levels. Calorie restriction reduced the levels of stored protein after …

Modern Biological Approaches To Folk Medicines And Traditional Antifungal Therapies, Matthew G. Jessulat, Robert P. Smith, Nadereh H. Mir-Rashed, Golshani Ashkan, John Thor Arnason, Myron L. Smith

Biology Faculty Articles

No abstract provided.

Emotions And Actions Associated With Altruistic Helping And Punishment, Omar Tonsi Eldakar, David Sloan Wilson, Rick O'Gorman

Biology Faculty Articles

Evolutionary altruism (defined in terms of fitness effects) exists in the context of punishment in addition to helping. We examine the proximate psychological mechanisms that motivate altruistic helping and punishment, including the effects of genetic relatedness, potential for future interactions, and individual differences in propensity to help and punish. A cheater who is a genetic relative provokes a stronger emotional reaction than a cheater who is a stranger, but the behavioral response is modulated to avoid making the transgression public in the case of cheating relatives. Numerous behavioral differences are not accompanied by emotional differences, suggesting that other psychological mechanisms …

Cortisol Concentrations In Human Skeletal Muscle Tissue After Phonophoresis With 10% Hydrocortisone Gel, Amanda R. Kuntz, Christine Multer Griffiths, James M. Rankin, Charles W. Armstrong, Thomas J. Mcloughlin

Biology Faculty Articles

CONTEXT: The delivery of hydrocortisone through phonophoresis is a widely prescribed technique for the treatment of various musculoskeletal inflammatory conditions. However, limited scientific evidence exists to support the efficacy of phonophoresis in delivering hydrocortisone to skeletal muscle tissue in humans.

OBJECTIVE: To determine hydrocortisone (cortisol) concentrations in human skeletal muscle tissue after a phonophoresis treatment using 10% hydrocortisone gel.

DESIGN: Randomized design in which 12 subjects were randomly assigned to either an ultrasound (sham) treatment or a 10% hydrocortisone phonophoresis treatment.

SETTING: Laboratory.

PATIENTS OR OTHER PARTICIPANTS: Twelve healthy subjects (8 women, 4 men: age = 22.3 +/- 2.64 years, …

Comparison Of Microbial Water Quality Parameters Of Four Geographically Similar Creeks In Northeast Tennessee, Kimberlee K. Hall, L. K. Gallagher, Brian G. Evanshen, Kurt J. Maier, Phillip R. Scheuerman

ETSU Faculty Works

Four creeks within the Watauga River watershed in Northeast Tennessee are routinely monitored for water quality assessments. To identify sources and monitor remediation, Sinking Creek, Cash Hollow Creek, Buffalo Creek and Boones Creek are monitored for chemical and microbial parameters. These parameters include phosphates, nitrates, BOD and fecal coliforms. Sinking Creek is a tributary of the Watauga River with 10 miles of impaired water. Cash Hollow Creek enters the Watauga River at river mile 11.4 with 3.4 miles of impaired water. Boones Creek contains 18.6 impaired miles while the status of water quality in Buffalo Creek is not yet determined. …

Development Of Proliferation Assays To Explore The Role Of Calcium Activated Potassium Channels In Cell Proliferation, Sean Foster

Honors Theses

Due to the large variety of K^† channel families implicated in cancer cell proliferation, it is desirable to develop techniques capable of quickly and reliably screening a variety of potential channels for a role in proliferation. While there are many established cell counting procedures, the time-consuming nature of such protocols limits their usefulness in broadly identifying particular channels in uncharacterized cell lines. For this reason, a primary goal of the present research was the development of techniques capable of implicating particular ion channels in proliferation with both qualitative and quantitative data. Once this goal was accomplished, the project sought …

Pineywoods Native Plant Center, Winter 2006, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.

3rd Lone Star Regional Native Plant Conference, David Creech, Lijing Zhou, Dawn Stover, James Kroll, Greg Grant, Heinz Gaylord

Lone Star Regional Native Plant Conference

No abstract provided.

Some Notes On The Early History Of The Robert B. Gordon Natural Area For Environmental Studies, David Fluri

Gordon Natural Area History & Strategic Plan Documents

No abstract provided.

Some Notes On The Early History Of The Robert B. Gordon Natural Area For Environmental Studies, Bradley M. Gottfried

Gordon Natural Area History & Strategic Plan Documents

No abstract provided.

Rockfish Resources Of The South Central California Coast: Analysis Of The Resource From Partyboat Data, 1980-2005, John Stephens, Dean Wendt, Debra Wilson-Vandenberg, Jay Carroll, Royden Nakamura, Erin Nakada, Steven Reinecke, Jono Wilson

Biological Sciences

Rockfishes (Sebastes spp.) have historically comprised a large proportion of catches in the nearshore recreational fishery in California, but declining populations of some species have led to increasingly restrictive management of the resource. This report summarizes new and existing data on rockfishes of the south central coast of California. In 2003, the California State Polytechnic University, San Luis Obispo placed observers on commercial passenger fishing vessels (partyboats) from the region. By the end of 2005, we had observed catches from 258 trips (8,839 fisher hours). We appended these data to partyboat catch statistics collected by the California Department of …

Characterizing The Resident, Fermentative Microbial Consortium In The Hindgut Of The Temperate-Zone Herbivorous Fish, Hermosilla Azurea (Teleostei: Kyphosidae), Pat M. Fidopiastis, Daniel J. Bezdek, Michael H. Horn, Judith S. Kandel

Biological Sciences

The zebraperch, Hermosilla azurea Jenkins and Evermann, a warm-temperate marine fish species with a strictly macroalgal diet, has a relatively long digestive tract with an enlarged hindgut and an associated blind caecum (HC). In zebraperch sampled off Santa Catalina Island, California (33°19′42′′N; 118°18′37′′W) in years 1995 through 2001, direct cell counts, gut epithelium assessment of bacterial attachment, and short-chain fatty acid (SCFA) analyses verified that the zebraperch HC possesses a dense and morphologically diverse, fermentative microbiota. Bacterial cell counts and morphological diversity were significantly higher in HC contents compared to anterior gut regions, suggesting that microbial populations were growing along …

Apples At A Glance From Western Australia, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

Western Australia produces a range of horticultural commodities including fruit, vegetables, flowers, nursery products and wine as part of its fast $670 million growing horticultural industry. Natural advantages such as climate and clean environment, soils and water make Western Australia an ideal place to supply a variety of high quality produce to domestic and international markets.

The apple industry in Western Australia has reached optimal yields from well established orchards. Production is currently estimated at a value of $37 million. Western Australia is the second major apple exporter in Australia. Around 20 per cent of the state's production of 37,745 …

A Guide For Skeleton Weed Management And Control, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

Assisting Western Australian landholders to eradicate skeleton weed and to prevent its further spread within the State

Sampling And Testing For Plant Pathogens, Aileen Reid

Bulletins 4000 -

There are many methods used in the laboratory to determine whether Phytophthora or other fungi are present in growing media, water supply and diseased plants. These include:

• filtering water for spores

• baiting for fungi in growing media and water samples

• examining diseased plant tissue microscopically

• direct culturing from roots and other plant parts to isolate the fungal pathogen in pure culture.

This bulletin outlines some of the procedures involved. Growers may be able to do some basic baiting of soil or water samples themselves to alert them of a problem but the procedures involved in isolating …

Wine From Western Australia At A Glance, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

Western Australia continues to enjoy a growing international reputation for the production of premium quality wines. Accolades at international wine exhibitions and in publications are abundant. Natural advantages such as the climate and soils, ideally suited to the production of premium quality wine grapes in a clean environment, make Western Australia an ideal place to supply quality wine to domestic and international markets.

Phytophthora Diseases Of Cutflower Crops, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

Phytophthora root rot is the most common soil borne disease causing plant death in native cut flower production. It is also a pathogen of exotic cutflower crops such as rose, lily, carnation, proteas and gerbera. The fungus, Phytophthora cinnamomi, the cause of jarrah dieback is the pathogen that first comes to mind when Phytophthora is mentioned. This has one of the widest host ranges of all Phytophthora species, particularly amongst native Australia species. P.nicotianae also has a wide host range, infecting a wide range of exotic, as well as Australian native flower crops. There are also a number of …

Brassicas From Western Australia At A Glance, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

Western Australia produces all of its domestic fresh broccoli, cabbage, cauliflower and Chinese cabbage requirements and is ideally positioned to service markets in Asia, the Indian Ocean region and beyond. Singapore, Malaysia and Brunei account for about 78 per cent of vegetable brassica exports from Western Australia. However, markets also exist in Hong Kong, Taiwan, Thailand, Japan, Indonesia and the Middle East.

Opportunities may also exist for supplying value added product such as fresh cut or frozen prepacked florets.The industry, supported by the Department of Agriculture and Food, is pursuing opportunities in productivity improvement, quality assurance and marketing which will …

A Novel Yogurt Product With Lactobacillus Acidophilus, Senthil Ganesh

LSU Master's Theses

Health benefits of Lactobacillus acidophilus include providing immune support for infections or cancer, providing a healthy replacement of good bacteria in the intestinal tract following antibiotic therapy, reducing occurrence of diarrhea in humans, aiding in lowering cholesterol and improving the symptoms of lactose intolerance. Consumer demand exists for new dairy products. There are several types of yogurt like stir curd, set curd and drinkable yogurt and they all need to be refrigerated. Moreover there are very few dairy products that can be stored at room temperature and not many dairy foods are finger foods. A novel yogurt product like a …

Color And Tenderness In Chilled Or Frozen Pork Loin Chops After Antioxident Dipping And Modified Atmosphere Packaging, Dennis Michael Price

LSU Master's Theses

The objective of this research was to evaluate color and tenderness in chilled or frozen pork loin chops after antioxidant dipping and modified atmosphere packaging. Loin chops were dipped in 0.3 M calcium chloride, 2.0 % sodium ascorbate, 0.2 M calcium ascorbate, or 0.3 M calcium ascorbate. Non-dipped chops served as controls. Chops were packaged in high oxygen (80% O2 / 20% CO2) or no oxygen (80% N2 / 20% CO2) and stored chilled (4° C) for 7 days or frozen (-18° C) for 21 days. After storage, chops were displayed under continuous fluorescent lighting for 3 or 6 days. …

A Comparative Study Of Household Demand For Meats By U.S. Hispanics, Carlos Ignacio Garcia

LSU Master's Theses

The general objective of this research project was to study the consumption behavior of U.S. Hispanics for meat products. A comprehensive review of literature on the studies of U.S. Hispanic consumers was performed by means of a systematic survey; different sources of information useful in studying Hispanic consumers were documented. The simultaneous increase in population and income of Hispanics in the United States is attractive to food companies, and makes more relevant the study of their consumer behavior patterns for discovering future market opportunities. Censored incomplete demand systems of the LinQuad form were used for recognizing the consumption patterns by …

Cryopreservation And Intracytoplasmic Sperm Injection With Bovine Epididymal Spermatozoa, Carlos Andres Guerrero

LSU Doctoral Dissertations

Recently, interest in the preservation of epididymal sperm as a potential source of valuable genes for genome resource banks has escalated. The development of a successful protocol to recover and cryopreserve sperm harvested from the epididymides would salvage germplasm from genetically valuable males that are injured and can no longer mate or have unexpectedly died and can be used as a model for the preservation of male gametes from endangered species. In a series of experiments, epididymal sperm was successfully harvested, cryopreserved and used for intracytoplasmic sperm injection. In Experiment I, ethylene glycol was found to cause significantly (P<0.05) less osmotic damage to bovine sperm during a one step addition and/or removal at 4°C as compared with glycerol in all concentrations evaluated. Furthermore, prolonged exposure (5 days at 4°C) of ethylene glycol was found to be less toxic than glycerol to sperm. In Experiment II, it was demonstrated that glycerol was more effective than ethylene glycol in providing protection against freezing injury during the cryopreservation process in the concentrations evaluated. In Experiment III, it was demonstrated that epididymal sperm retrieval using seminal plasma is beneficial to enhance sperm overall and progressive motility characteristics and to protect it from morphological abnormalities derived from the freezing process. In Experiment IV, a one step dilution process for removal of glycerol from cryopreserved epididymal sperm was found to significantly affect plasma membrane integrity and mitochondrial function of sperm previously exposed to seminal plasma. However, seminal plasma exposure did not have any significant detrimental effect on acrosome integrity. Furthermore, it was demonstrated that the longevity and survivability in vitro during a 4-hour incubation period at 37°C of post-thaw epididymal sperm exposed to seminal plasma prior to cryopreservation was not compromised when compared with the control extended sperm. In Experiment V, we have demonstrated that fertilization, blastocyst and fetal development could be achieved with cryopreserved bovine epididymal sperm by intracytoplasmic sperm injection (ICSI). To our knowledge, this is the first report in the United States and second in the world to use bovine epididymal sperm for ICSI. We achieved far markedly improved blastocyst rates over those results recently reported in the first study originating in Japan.

Evaluation On Antioxidant Activities Of The Soybean Oils And Gums, Xianglong Yuan

LSU Master's Theses

In this study crude soy oil was extracted from the soy flour by hexane solvent. The crude oil was refined using a refining procedure similar to the one in edible oil industries, which included degumming, neutralizing, and bleaching. As the result, the eight groups of the oils and the gums were obtained. The compositions of fatty acids and tocopherols in the eight groups of samples were analyzed using GC-FID and HPLC, respectively. The antioxidant activities of the samples were analyzed by two chemical models, cholesterol and DHA. The results showed that ã- and ä-tocopherols may not be the main antioxidants …

Interview With Myo Demayo, Urbanstead Farm (Audio), Myo Demayo

All Sustainability History Project Oral Histories

Interview of Myo DeMayo by Tyler Anderson.

The interview index is available for download.

The year is estimated.

Variation In The Response Of Crustacean Zooplankton Species Richness And Composition To The Invasive Predator Bythotrephes Longimanus, Angela L. Strecker, Shelley E. Arnott, Norman D. Yan, Robert Girard

Environmental Science and Management Faculty Publications and Presentations

The predacious invertebrate Bythotrephes longimanus has invaded >90 freshwater lakes in North America. There is some evidence that B. longimanus has a negative effect on summer zooplankton species richness; however, no study has examined the effect of B. longimanus throughout the ice-free season in more than one lake. We visited 10 invaded and 4 reference lakes every 2 weeks from May to September, collecting B. longimanus, crustacean zooplankton, and water chemistry samples. Composite samples were pooled across the study season for each lake. Bythotrephes longimanus significantly reduced cladoceran species richness, diversity, and abundance, and the total zooplankton community also exhibited …

Neural Induction And Differentiation Of Stem Cells Using The Developmental Gene Pax7, Vijesh G. Vaghjiani

Theses : Honours

Neurodegenerative disorders are conditions that mainly affect the brain and the central nervous system (CNS). Each disease type is characterised by loss of function which results from death of a particular region. Interestingly enough, most of the time disease pathology is due to loss of specific cell types in the CNS. These pathological conditions have both high economic costs and social implications for society. To treat such conditions, either the lost cells must be replaced or the cells surrounding the damaged tissue must be induced to undergo repair to replace the lost cells. The former looks more achievable whereas the …

Potential Of Vibrational Spectroscopy In The Diagnosis Of Human Tumours., Eoghan O'Faolain

Doctoral

Just fewer than 20,000 people are annually diagnosed with some form of cancer in Ireland and one in three people are likely to contract some form of cancer by age 74. With the number of cases increasing at an annual rate of 2%, the early detection and treatment of cancer is becoming increasingly important. Both IR and Raman spectroscopy offer the potential for real time, quantitative detection of cancer and even precancer. This study investigates the potential of Raman and Fourier transform infrared, both benchtop and synchrotron spectroscopies for the detection of cervical cancer. The tissue was classified and its …

Isolation And Characterization Of Toxin A-Negative, Toxin B-Positive Clostridium Difficile In Dublin, Ireland, Denise Drudy, N. Harnedy, S. Fanning, R. O’Mahony, L. Kyne

Articles

Clostridium difficile is a major cause of infectious diarrhoea in hospitalised patients. Most pathogenic C. difficile strains produce two toxins, A and B; however, clinically relevant toxin A-negative, toxin Bpositive (A– B+ ) strains of C. difficile that cause diarrhoea and colitis in humans have been isolated worldwide. The aims of this study were to isolate and characterise A– B+ strains from two university hospitals in Dublin, Ireland. Samples positive for C. difficile were identified daily by review of ELISA results and were cultured on selective media. Following culture, toxin-specific immunoassays, IMR-90 cytotoxicity assays and PCR were used to analyse …

Anti-Herpes Simplex Virus Type 2 Activities Of Some Thai Medicinal Plants, Mali Kruawan, Sakulrat Rattanakiat

The Thai Journal of Pharmaceutical Sciences

To explore for plants possessing anti-herpes simplex virus type 2 (anti-HSV-2) activity, 80 extracts prepared from 16 medicinal plants collected in Thailand were investigated in vitro using the plaque reduction assay including the inactivation, pre-treat...

Assessment Of Client Satisfaction In Six Urban Wic Clinics, Catherine Christie, Julia A. Watkins, Anita Martin, Helen Jackson, Judy E. Perkin, Jacquie Fraser

Nutrition and Dietetics Faculty Research and Scholarship

Customer service is a measure of support and courtesy provided to individuals who patronize an organization, and is a factor vital to the success of any business. Programs that strive to meet critical needs of at risk populations, such as the Special Supplementation Nutrition Program for Women, Infants, and Children (WIC), may also benefit from assessment of client satisfaction. The purpose of the study was to examine factors related to customer satisfaction in Duval County Florida WIC clinics and identify potential barriers to participation through a two-year project initiated by the health department and the state university. The study examined …