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2006

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Articles 2551 - 2580 of 3150

Full-Text Articles in Life Sciences

Effect Of Calcium Lactate And Heat-Shock On Texture In Fresh-Cut Lettuce During Storage, Ana Belen Martin-Diana, Daniel Rico, Jesus Maria Frias, Gary Henehan, Jemina Mulcahy, J. Barat, Catherine Barry-Ryan Jan 2006

Effect Of Calcium Lactate And Heat-Shock On Texture In Fresh-Cut Lettuce During Storage, Ana Belen Martin-Diana, Daniel Rico, Jesus Maria Frias, Gary Henehan, Jemina Mulcahy, J. Barat, Catherine Barry-Ryan

Articles

Textural and microstructural changes in fresh-cut lettuce were analysed over 12 days storage. The vegetable was treated with 120 ppm chlorine and with 15 g/L calcium lactate at room temperature (18–20 °C) and at 50 °C (heat-shock). Texturometer analysis showed that samples washed with calcium lactate had significantly (p > 0.05) higher crispness values than samples washed with chlorine. However the use of 50 °C treatment (heat-shock) gave better textural properties at the end of storage and significantly retarded the softening process, being in agreement with the sensorial results. Cryo-SEM micrographs showed a loss of turgor (shrinkage) of the tissue cells …

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Horseradish And Soybean Peroxidases: Comparable Tools For Alternative Niches?, Barry Ryan, CiaráN Fagan, Neil Carolan Jan 2006

Horseradish And Soybean Peroxidases: Comparable Tools For Alternative Niches?, Barry Ryan, CiaráN Fagan, Neil Carolan

Articles

Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful biotechnological tools. HRP is often termed the classical plant heme peroxidase, and although it has been studied for decades our understanding has deepened since its cloning and subsequent expression, which has enabled numerous mutational and protein engineering studies. SBP, however, has been neglected until recently; despite offering a real alternative to HRP that actually outperforms it in terms of stability. SBP is now used in numerous biotechnological applications, including biosensors. Review of both is timely. This article summarises and discusses the main insights into the structure and mechanism of HRP, …

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Antimicrobial Resistance In Non-Typhoidal Salmonella From Food Sources Colombia: Evidence For An Unusual Plasmid Located Class 1 Integron In Serotypes Typhimurium And Anatum, Ciara Walsh, Rebecca O'Mahoney, Teresa Quinn, Denise Drudy, Paul Whyte, Salim Matar, Seamus Fanning Jan 2006

Antimicrobial Resistance In Non-Typhoidal Salmonella From Food Sources Colombia: Evidence For An Unusual Plasmid Located Class 1 Integron In Serotypes Typhimurium And Anatum, Ciara Walsh, Rebecca O'Mahoney, Teresa Quinn, Denise Drudy, Paul Whyte, Salim Matar, Seamus Fanning

Articles

Seventy-two isolates representing 18 serotypes recovered from various food samples collected in Colombia were tested for antimicrobial susceptibilities. The collection was further characterized for extended-spectrum cephalosporin, aminoglycoside, and tetracycline resistance markers. Multidrug resistant (MDR) isolates were further investigated for class 1 integrons and were evaluated for the presence of conjugative plasmids along with a determination of the incompatibility group by polymerase chain reaction (PCR). Antibiogram analysis showed that the incidence rate of ceftiofur resistance was moderately high (15%). A similar level of resistance to neomycin and oxytetracycline (11% and 10%, respectively) was also observed. There was a high prevalence of …

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Antimicrobial Resistance In Irish Isolates Of Verocytotoxigenic Escherichia Coli (E. Coli)-Vtec., Ciara Walsh, Geraldine Duffy, R. O'Mahoney, I. S. Blair, D. A. Mcdowell Jan 2006

Antimicrobial Resistance In Irish Isolates Of Verocytotoxigenic Escherichia Coli (E. Coli)-Vtec., Ciara Walsh, Geraldine Duffy, R. O'Mahoney, I. S. Blair, D. A. Mcdowell

Articles

This study compared the antimicrobial resistance profiles of Escherichia coli O157:H7 isolates (n = 257) recovered from bovine hides, minced beef and human clinical samples in Ireland, to those profiles of a range of Irish non-O157 E. coli (O111 and O26) isolates (n=31) from a variety of clinical and veterinary sources. Four multi-drug resistant (MDR) E. coli O157:H7 food isolates were identified, with resistance to 10 (1 isolate), 6 (1 isolate) and 4 (2 isolates) antimicrobial agents respectively. Two of these isolates (resistant to 7 and 4 antimicrobial classes) were characterised further by molecular methods and found to contain class …

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Survival Of Antibiotic Resistant And Antibiotic Sensitive Strains Of E. Coli O157 And E. Coli O26 In Food Matrices., Ciara Walsh, Geraldine Duffy, I. S. Blair, D. A. Mcdowell Jan 2006

Survival Of Antibiotic Resistant And Antibiotic Sensitive Strains Of E. Coli O157 And E. Coli O26 In Food Matrices., Ciara Walsh, Geraldine Duffy, I. S. Blair, D. A. Mcdowell

Articles

Escherichia coli O157:H7 or E. coli O26, which were AS (antibiotic sensitive), AR (laboratory created antibiotic resistant mutants), or naturally MAR (multi-antibiotic resistant), were inoculated into laboratory media, yoghurt or orange juice and their growth/survival monitored during enrichment at 37 °C or storage at 4 °C. The strains were also inoculated into minced beef and their thermal inactivation (D-values) examined at 55 °C, with and without a prior heat shock at 48 °C. The growth kinetics (lag phases, growth rates) of the VTEC (verocytotoxigenic E. coli), incubated over 24 h at 37 °C in laboratory media, were …

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The Effect Of Tgf-B And Egf On Mmp-2 Activation And Mt1-Mmp Phosphorylation In U87mg And T98g Human Glioma Cells, Jacquelyn B. Knupp Jan 2006

The Effect Of Tgf-B And Egf On Mmp-2 Activation And Mt1-Mmp Phosphorylation In U87mg And T98g Human Glioma Cells, Jacquelyn B. Knupp

Honors Theses

Glioblastoma multiforme (GBM), a high-grade, malignant brain tumor of astrocyte origin, invades normal brain tissue and most often results in death ofthe patient withinayearofdiagnosis. In order to invade the surrounding tissue and thereby metastasize, these tumor cells must migrate through the extracellular matrix. The pmpose ofthis research project is to investigate the family ofmatrix metalloproteinases responsible for extracellular degradation. Specifically, this investigation looks at MMP-2 activation via MT 1-MMP and the possible importance of phosphorylation of the tyrosine residue on the cytoplasmic tail of MTl-MMP. U87wildtypegliomacellsaswellasT98 p53 mutant glioma cells were treated with transforming growth factor beta (TGF-P). Preliminary results …

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Humboldt Journal Of Microbiology: Volume 8, Number 1- May 2006, Hsu Press Jan 2006

Humboldt Journal Of Microbiology: Volume 8, Number 1- May 2006, Hsu Press

Humboldt Journal of Microbiology

No abstract provided.

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Leeches (Annelida: Hirudinida) Of Northern Arkansas, William E. Moser, Donald J. Klemm, Dennis J. Richardson, Benjamin A. Wheeler, Stanley E. Trauth, Bruce A. Daniels Jan 2006

Leeches (Annelida: Hirudinida) Of Northern Arkansas, William E. Moser, Donald J. Klemm, Dennis J. Richardson, Benjamin A. Wheeler, Stanley E. Trauth, Bruce A. Daniels

Journal of the Arkansas Academy of Science

Twenty-one lotic and lentic environments throughout central and northern Arkansas were surveyed for the presence of leeches during June 2004 and April, July - October 2005. Fourteen species of leeches {Helobdella elongata, Helobdella papillata, Helobdella stagnalis, Placobdella cryptobranchii, Placobdella multilineata, Placobdella ornata, Placobdella papillifera, Placobdella parasitica, Placobdella phaler a, Placobdella picta, Haemopis marmorata, Erpobdella fervida, Erpobdella microstoma, and Erpobdella punctata) representing 3 families were collected. Five species (H. elongata, P. cryptobranchii, P. multilineata, H. marmorata, and E. fervida) are reported from Arkansas for the first time. The natural history of the 22 species of leeches now known from Arkansas is …

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Discovery Of A Dicephalic Western Diamondback Rattlesnake, Crotalus Atrox (Serpentes: Viperidae), From Texas, With A Summary Of Dicephalism Among Members Of The Genus Crotalus, Chris T. Mcallister, Van Wallach Jan 2006

Discovery Of A Dicephalic Western Diamondback Rattlesnake, Crotalus Atrox (Serpentes: Viperidae), From Texas, With A Summary Of Dicephalism Among Members Of The Genus Crotalus, Chris T. Mcallister, Van Wallach

Journal of the Arkansas Academy of Science

We document the fourth record of a dicephalic western diamondback rattlesnake, Crotalus atrox, collected on 17 September 2005 in Tom Green County, Texas, along with features of its external and internal anatomy, which are compared with normal C. atrox neonates. This is only the second reported case of complete anatomical duplication of the respiratory system and nearly complete duplication of the circulatory and digestive systems in dicephalic snakes. In addition, we present a summary of dicephalism among 10 species within the genus Crotalus.

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Evaluation Of Cross Pollination Of Zephyranthes And Habranthus Species And Hybrids, Moytri Roy Chowdhury, John Hubstenberger Jan 2006

Evaluation Of Cross Pollination Of Zephyranthes And Habranthus Species And Hybrids, Moytri Roy Chowdhury, John Hubstenberger

Journal of the Arkansas Academy of Science

The genus Zephyranthes and the related genus Habranthus in the family Amaryllidaceae are commonly grown ornamental bulbs having new world homologies. Inter-specific and inter-generic hybridizations are complicated by the fact that some of the species are apomictic, cross incompatible, or have widely variable 2n chromosome numbers. A simple, inexpensive method of pollen storage was tested to evaluate the production of hybrid seed. Intact anthers harvested after pollen release and stored at 4°C were used for pollination. Emasculated flowers were pollinated at noon on the first or second day of anthesis. Hybrid crosses were labeled and seed collected when formed. All …

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Geographic Distribution And Harvest Dynamics Of The Eastern Spotted Skunk In Arkansas, D. Blake Sasse, Matthew E. Gompper Jan 2006

Geographic Distribution And Harvest Dynamics Of The Eastern Spotted Skunk In Arkansas, D. Blake Sasse, Matthew E. Gompper

Journal of the Arkansas Academy of Science

The eastern spotted skunk (Spilogale putorius) is a small carnivore found across much of the central and southeastern United States, and while once common, this species has become rare in most obits range. We used harvest records collected by the Arkansas Game and Fish Commission from 1941-2004 to examine historic and current distribution and long-term harvest dynamics of this species in Arkansas. Eastern spotted skunks have historically been most common in the Ozarks and the Ouachitas though the species appears to have been present, but uncommon, in the Gulf Coastal Plain and in some counties in southeastern Arkansas near the …

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New Evidence For A Maternity Colony Of The Indiana Bat (Myotis Sodalis) In The Delta Of Arkansas, Stephen C. Brandebura, Rex E. Medlin Jr., Thomas S. Risch Jan 2006

New Evidence For A Maternity Colony Of The Indiana Bat (Myotis Sodalis) In The Delta Of Arkansas, Stephen C. Brandebura, Rex E. Medlin Jr., Thomas S. Risch

Journal of the Arkansas Academy of Science

No abstract provided.

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Vascular Flora Of The South Fork Native Plant Reserve, Van Buren County, Arkansas, Theo Witsell, Brent Baker Jan 2006

Vascular Flora Of The South Fork Native Plant Reserve, Van Buren County, Arkansas, Theo Witsell, Brent Baker

Journal of the Arkansas Academy of Science

The South Fork Native Plant Preserve, a property owned and managed by the Gates Rogers Foundation, Inc., is located along the South Fork of the Little Red River (now Greers Ferry Lake) in Van Buren County, Arkansas. We conducted a floristic inventor/ of a 61.3 ha study area consisting of the preserve, adjacent lakefront property owned by the U.S. Army Corps of Engineers, and a riparian corridor on neighboring land from February through November 2005. A total of 582 vascular plant taxa, representing 118 families and 336 genera, was documented including six species (Carex lupuliformis Sartwell ex L.H.Dewey, Carex swanii …

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Unusual Prey Item Found In An Eastern Bluebird Nest, Sarah De Viney Baxter, Richard J. Baxter, Thomas S. Risch Jan 2006

Unusual Prey Item Found In An Eastern Bluebird Nest, Sarah De Viney Baxter, Richard J. Baxter, Thomas S. Risch

Journal of the Arkansas Academy of Science

No abstract provided.

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Distribution Of Arkansas's Bottomland Bats, Rex E. Medlin Jr., Stephen C. Brandebura, H. Bobby Fokidis, Thomas S. Risch Jan 2006

Distribution Of Arkansas's Bottomland Bats, Rex E. Medlin Jr., Stephen C. Brandebura, H. Bobby Fokidis, Thomas S. Risch

Journal of the Arkansas Academy of Science

No abstract provided.

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Hyperinfection With The Bass Tapeworm, Proteocephalus Ambloplites (Cestoda), In The Black Basses Micropterus Punctulatus And M. Dolomieui From Certain Arkansas Reservoir Lakes, James J. Daly Sr., Randal J. Keller, Bruce Deyoung Jan 2006

Hyperinfection With The Bass Tapeworm, Proteocephalus Ambloplites (Cestoda), In The Black Basses Micropterus Punctulatus And M. Dolomieui From Certain Arkansas Reservoir Lakes, James J. Daly Sr., Randal J. Keller, Bruce Deyoung

Journal of the Arkansas Academy of Science

No abstract provided.

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New Distributional Records Of Lampreys From Arkansas, Henry W. Robison, C. Renn Tumlison, James C. Petersen Jan 2006

New Distributional Records Of Lampreys From Arkansas, Henry W. Robison, C. Renn Tumlison, James C. Petersen

Journal of the Arkansas Academy of Science

No abstract provided.

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Cajun Dwarf Crawfish (Cambrellus Shufeldtii): An Intermediate Host For Southwellina Dimorpha (Acanthocephala), Dennis J. Richardson, William F. Font Jan 2006

Cajun Dwarf Crawfish (Cambrellus Shufeldtii): An Intermediate Host For Southwellina Dimorpha (Acanthocephala), Dennis J. Richardson, William F. Font

Journal of the Arkansas Academy of Science

No abstract provided.

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Complex Stand On The White River National Wildlife Refuge: Implications For Bottomland Hardwood Old Growth, Brian Roy Lockhart, James E. Kellum Jan 2006

Complex Stand On The White River National Wildlife Refuge: Implications For Bottomland Hardwood Old Growth, Brian Roy Lockhart, James E. Kellum

Journal of the Arkansas Academy of Science

No abstract provided.

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Five Years Of Change In An Old-Growth Pine-Hardwood Remnant In Ashley County, Arkansas, Don C. Bragg Jan 2006

Five Years Of Change In An Old-Growth Pine-Hardwood Remnant In Ashley County, Arkansas, Don C. Bragg

Journal of the Arkansas Academy of Science

The Levi Wilcoxon Demonstration Forest near Hamburg, Arkansas is an industrially-owned remnant of old-growth pine and hardwoods. Some of the loblolly (Pinus taeda L.)and shortleaf (Pinus echinata Mill.)pine in this stand are over 200 years old, and numerous individuals exceed 90 cm in diameter and 30 m in height. A2000 survey of a portion of this tract found that 27 tree species contributed an average of387.5 live stems/ha and 31.8 m2 /ha of basal area. An inventory of the same plots in2006 yielded noticeable declines in density (now down to 342.5 stems/ha) and basal area (now 28.2 m2 /ha). Much …

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Eye Development At The Houston "Fly Meeting", Amit Singh Jan 2006

Eye Development At The Houston "Fly Meeting", Amit Singh

Biology Faculty Publications

Meeting report: The 47th Annual Drosophila Research Conference or "Fly Meeting" took place at Houston, Texas, USA from March 29th- April 2nd, 2006, under the aegis of the Genetics Society of America. The Fly Meeting provides an excellent opportunity for fly researchers to present their work and to get a snapshot of recent developments and upcoming trends in their research field. The fruit fly, Drosophila melanogaster, is a very versatile model to study growth, patterning, neural development, evolution, systemetics and various other facets of biomedical science. The topics presented in the meeting covered a very broad spectrum of fly research. …

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Gene Expression And Discovery During Lens Regeneration In Mouse: Regulation Of Epithelial To Mesenchymal Transition And Lens Differentiation, Mario Medvedovic, Craig R. Tomlinson, Mindy Kay Call, Matthew Grogg, Panagiotis A. Tsonis Jan 2006

Gene Expression And Discovery During Lens Regeneration In Mouse: Regulation Of Epithelial To Mesenchymal Transition And Lens Differentiation, Mario Medvedovic, Craig R. Tomlinson, Mindy Kay Call, Matthew Grogg, Panagiotis A. Tsonis

Biology Faculty Publications

Purpose: It has been shown that after extracapsular lens removal by anterior capsulotomy in the mouse, the lens can be regenerated. However, as the capsular bag is filled with fibers, epithelial to mesenchymal transition (EMT), an event which is common after cataract surgery as well, takes place during early stages. This study, using a unique mouse model, was undertaken to identify novel regulators and networks in order to more clearly understand secondary cataracts at the molecular level.

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Comparison Of Dot Chromosome Sequences From D. Melanogaster And D. Virilis Reveals An Enrichment Of Dna Transposon Sequences In Heterochromatic Domains, Elizabeth Slawson, Christopher Shaffer, Colin Malone, Wilson Leung, Elmer Kellmann, Rachel Shevchek, Carolyn Craig, Seth Bloom, James Bogenpohl 2nd, James Dee, Emiko Morimoto, Jenny Myoung, Andrew Nett, Fatih Ozsolak, Mindy Tittiger, Andrea Zeug, Mary-Lou Pardue, Jeremy Buhler, Elaine Mardis, Sarah C.R. Elgin Jan 2006

Comparison Of Dot Chromosome Sequences From D. Melanogaster And D. Virilis Reveals An Enrichment Of Dna Transposon Sequences In Heterochromatic Domains, Elizabeth Slawson, Christopher Shaffer, Colin Malone, Wilson Leung, Elmer Kellmann, Rachel Shevchek, Carolyn Craig, Seth Bloom, James Bogenpohl 2nd, James Dee, Emiko Morimoto, Jenny Myoung, Andrew Nett, Fatih Ozsolak, Mindy Tittiger, Andrea Zeug, Mary-Lou Pardue, Jeremy Buhler, Elaine Mardis, Sarah C.R. Elgin

Biology Faculty Research

Background: Chromosome four of Drosophila melanogaster, known as the dot chromosome, is largely heterochromatic, as shown by immunofluorescent staining with antibodies to heterochromatin protein 1 (HP1) and histone H3K9me. In contrast, the absence of HP1 and H3K9me from the dot chromosome in D. virilis suggests that this region is euchromatic. D. virilis diverged from D. melanogaster 40 to 60 million years ago. Results: Here we describe finished sequencing and analysis of 11 fosmids hybridizing to the dot chromosome of D. virilis (372,650 base-pairs) and seven fosmids from major euchromatic chromosome arms (273,110 base-pairs). Most genes from the dot chromosome of …

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Zinc Content In The Organs And Tissues Of Freshwater Fish From The Kardjali And Studen Kladenets Dam Lakes In Bulgaria, Iliana G. Velcheva Jan 2006

Zinc Content In The Organs And Tissues Of Freshwater Fish From The Kardjali And Studen Kladenets Dam Lakes In Bulgaria, Iliana G. Velcheva

Turkish Journal of Zoology

A study was conducted to estimate zinc loads in the organs and tissues of Alburnus alburnus, Cyprinus carpio and Perca fluviatilis inhabiting 2 dam lakes Kardjali and Studen Kladenets, located along the Arda river (South Bulgaria). The zinc content was determined by atomic absorption spectrophotometry using a Perkin Elmer 380 B apparatus .The effect of the temporal factor was proved over a 3-year study. The highest zinc concentrations in fish organs and tissues were detected in summer and autumn. The highest zinc loads were found in the kidney and liver, and the lowest in the muscles of the 3 fish …

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Gis And 3d Analysis Applied To Sea Turtle Mortalities And Navigation Channel Dredging, Bradley A. Shellito, Keith B. Lockwood Jan 2006

Gis And 3d Analysis Applied To Sea Turtle Mortalities And Navigation Channel Dredging, Bradley A. Shellito, Keith B. Lockwood

Virginia Journal of Science

Between 2000 and 2003 there were an increased number of documented sea turtle mortalities related to hopper dredging in the channels of the Chesapeake Bay. A pilot study was undertaken to create a bathymetric surface and three-dimensional model of the Cape Henry Channel using Geographic Information Systems (GIS) as a visualization tool to examine sea turtle mortalities in relation to the dredging. In Fall 2003, the US Army Corps of Engineers dredged the Thimble Shoals Federal Navigation Channel, and a more refined model was developed using this data. This project examines the growing concerns over sea turtle mortality rates and …

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Primary Structure Of The Carbohydrate-Binding Modules In Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates, Melek Özkan, Gülay Özcengi̇z Jan 2006

Primary Structure Of The Carbohydrate-Binding Modules In Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates, Melek Özkan, Gülay Özcengi̇z

Turkish Journal of Biology

In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, …

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Chinese Flame Tree (Koelreuteria Bipinnata Franch.) (Sapindaceae) New To The Arkansas Flora, Brett Serviss, Nicole Freeman, Sara Melancen Jan 2006

Chinese Flame Tree (Koelreuteria Bipinnata Franch.) (Sapindaceae) New To The Arkansas Flora, Brett Serviss, Nicole Freeman, Sara Melancen

Journal of the Arkansas Academy of Science

No abstract provided.

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Comparison Of Different Irrigation Methods Based On The Parametric Evaluation Approach, Orhan Dengi̇z Jan 2006

Comparison Of Different Irrigation Methods Based On The Parametric Evaluation Approach, Orhan Dengi̇z

Turkish Journal of Agriculture and Forestry

The main objective of this research is to compare two different irrigation methods according to parametric evaluation system in the Field Plants Central Research Institute-Ikizce Research Farm's soils located in southern Ankara. Soil properties of the study area including texture, depth, EC, drainage, carbonate content and slope were derived from a detailed soil map scaled 1/5000. After analyzing and evaluating soil properties using geographic information system techniques, gravity and drop irrigation suitability maps were generated. Results showed that 13.1% of the study area was highly suitable for surface and gravity irrigation methods, whereas 51.2% of the study area was highly …

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Effects Of Inoculation With Rhizobium On Seed Yield And Yield Components Of Common Vetch (Vicia Sativa L.), Sebahatti̇n Albayrak, Cafer Sirri Sevi̇may, Özgür Töngel Jan 2006

Effects Of Inoculation With Rhizobium On Seed Yield And Yield Components Of Common Vetch (Vicia Sativa L.), Sebahatti̇n Albayrak, Cafer Sirri Sevi̇may, Özgür Töngel

Turkish Journal of Agriculture and Forestry

The effects of inoculation with Rhizobium leguminosarum on seed yield and yield components of common vetch (Vicia sativa L.) were evaluated under Black Sea Coastal Region conditions, Turkey, in the 2001-2002 and 2002-2003 growing seasons using common vetch cultivars Kubilay, Ürem, Karaelçi, Uludağ, Emir, Çubuk and Nilüfer. The experiment was established as a split block design with 3 replications. Inoculation increased seed yield and all yield components of common vetch cultivars. It was observed that inoculated common vetch cultivars gave higher biological yield (8.5%), seed yield (7.6%), straw yield (10.4%), pod length (25.5%), number of seed per pod (16.2%), number …

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Study On Vertical And Lateral Leaching Of Nitrate From A Wheat Field In China, Md. Toufiq Iqbal Jan 2006

Study On Vertical And Lateral Leaching Of Nitrate From A Wheat Field In China, Md. Toufiq Iqbal

Turkish Journal of Agriculture and Forestry

Vertical and lateral leaching of nitrate from a wheat field was studied through one season in a field in China by applying increasing amount of N fertilizers (e.g. 90, 180, 270 and 360 kg ha^{-1}). Results showed that nitrate leaching was the dominating way of nitrate loss from wheat land during the first two month after sowing seeds. After irrigation, the nitrate concentration in the leachate of 30 cm soil layer was higher than that of 60 cm, while the concentration of nitrate leaching at the five N treatments through the two depth soil layer came into the same level …

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