Life Sciences Commons^™

Visualizing Early Frog Development With Motion-Sensitive 3-D Optical Coherence Microscopy, Richard C. Haskell, Mary E. Williams, Daniel C. Petersen, Barbara M. Hoeling, Andrew J. Schile, J. D. Pennington, M. G. Seetin, J. M. Castelaz, Scott E. Fraser, Cyrus Papan, Hongwu Ren, Johannes F. De Boer, Zhongping Chen

All HMC Faculty Publications and Research

A motion-sensitive en-face-scanning 3-D optical coherence microscope (OCM) has been designed and constructed to study critical events in the early development of plants and animals. We describe the OCM instrument and present time-lapse movies of frog gastrulation, an early developmental event in which three distinct tissue layers are established that later give rise to all major organ systems. OCM images constructed with fringe-amplitude data show the mesendoderm migrating up along the blastocoel roof, thus forming the inner two tissue layers. Motion-sigma data, measuring the random motion of scatterers, is used to construct complementary images that indicate the presence of Brownian …

Folate Biofortification In Tomatoes By Engineering The Pteridine Branch Of Folate Synthesis, Rocio Diaz De La Garza, Eoin P. Quinlivan, Sebastian M. J. Klaus, Gilles J. C. Basset, Jesse F. Gregory Iii, Andrew D. Hanson

Center for Plant Science Innovation: Faculty Publications

Plants are the main source of folate in human diets, but many fruits, tubers, and seeds are poor in this vitamin, and folate deficiency is a worldwide problem. Plants synthesize folate from pteridine, p-aminobenzoate (PABA), and glutamate moieties. Pteridine synthesis capacity is known to drop in ripening tomato fruit; therefore, we countered this decline by fruit-specific overexpression of GTP cyclohydrolase I, the first enzyme of pteridine synthesis. We used a synthetic gene based on mammalian GTP cyclohydrolase I, because this enzyme is predicted to escape feedback control in planta. This engineering maneuver raised fruit pteridine content by 3- …

Availability Of Ground Water Resources In The Piscataqua And Coastal Watersheds, Chormann, R, Frederick Chormann Jr.

PREP Reports & Publications

The following report summarizes progress on the the various components of the Availability of Groundwater Resources in the Piscataqua and Coastal Watershed poject and the status of deliverables as of June 30, 2004. Most tasks have been completed under this “data collection” phase of the overall, multi-year project, although some activities will continue simultaneously with the “data analysis” phase.

Involvement Of Raft-Like Plasma Membrane Domains Of Entamoeba Histolytica In Pinocytosis And Adhesion, Richard C. Laughlin, Glen C. Mcgugan, Rhonda R. Powell, Brenda H. Welter, Lesly A. Temesvari

Publications

Lipid rafts are highly ordered, cholesterol-rich, and detergent-resistant microdomains found in the plasma membrane of many eukaryotic cells. These domains play important roles in endocytosis, secretion, and adhesion in a variety of cell types. The parasitic protozoan Entamoeba histolytica, the causative agent of amoebic dysentery, was determined to have raft-like plasma membrane domains by use of fluorescent lipid analogs that specifically partition into raft and nonraft regions of the membrane. Disruption of raft-like membrane domains in Entamoeba with the cholesterol-binding agents filipin and methyl-β-cyclodextrin resulted in the inhibition of several important virulence functions, fluid-phase pinocytosis, and adhesion to host …

Recognizing And Overcoming Difficult Site Conditions For Afforestation Of Bottomland Hardwoods, William Conner, J A. Stanturf, E S. Gardiner, C J. Schweitzer, A W. Ezell

Publications

In the last decade, about 370,000 acres (150,000 ha) of economically marginal
farmland in the Lower Mississippi Alluvial Valley (LMAV) have been restored to
bottomland hardwood forests (Stanturf and others 1998, King and Keeland 1999,
Schoenholtz and others 2001). Planting of this considerable acreage is due to several federal programs, such as the Wetlands Reserve Program (WRP), that assist landowners by financing afforestation (Figure 1). Unfortunately, these operational plantings have not performed as well as smaller plantings or research plots (Stanturf and others 2001a). For example, a recent survey of WRP plantings in westcentral Mississippi revealed that more than 90 …

Historical Evidence Of Riparian Forests In The Great Plains And How That Knowledge Can Aid With Restoration And Management., Elliot West, Greg Ruark

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

While some argue that the Great Plains were dominated by grasslands and that riparian woodlands were rare, others contend that trees would logically have occurred in riparian areas due to favorable microenvironment conditions. Historically, what native plant communities were found in riparian zones of the Great Plains? The answers to this question depend to a large extent on what time period is used as a reference of pre-settlement conditions.

The U.S. Fish and Wildlife Service (1981) drew upon conditions in 1905 for insight and concluded that trees were “wholly absent” or consisted of scattered cottonwood and willow. However, such a …

Nebline, September 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

What if You Could Access Extension’s Educational Resources at the Push of a Button?
Sorting Through Internet Clutter
What Users Are Saying
Garden Guide: Things to do this month
Good, Low-Maintenance Trees to Plant
Herbal Festival
Aster Yellows on Purple Coneflower
Ladybugs, Ladybugs, Fly Away Home! Please!
Composting Indoors with Worms
Upcoming Household Hazardous Waste Collections
Packed Lunch Pizzazz!
Sports Mix Recipe
Adding A Rainbow To Your Diet
President’s Notes — Janet’s Jargon
Household Hints: Chill Out with an Efficient Fridge
FCE News & Events
Stress Affects Adolescents Too
Plan Now for Holiday Spending
Values
St. Johnswort is Increasing in …

Nebraska Vine Lines, Vol. Vii, No. 5, September/October 2004.

Nebraska Vine Lines (2003–2006)

Contents

• Fall Workshop: Instrumentation for Vineyards and Wineries

• Electronic Nose Technologies: A New Instrumentation Option for Wineries?

• Just Getting Started – or “So You Want to Grow Grapes”

• Fall Vineyard Management By Paul Read, Viticulture Professor

• FUNDAMENTALS OF WINE EVALUATION

• Summer Closes with a Bang

Immunity In Medicine, M Saeed

Department of Biological & Biomedical Sciences

No abstract provided.

Forage News [2004-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forage Spokesman Nominations
• Kentucky Grazing Conference
• Forages at KCA
• Switchgrass and Horses
• Cutting Alfalfa in Autumn Increases Annual Yield, Doesn’t Hurt Stands, but Is Unlikely to Increase Profits
• Nitrogen Rate and Source Effects on the Yield and Nutritive Value of Stockpiled Tall Fescue
• Stewardship for the 21^ST Century: A Report on America’s Forage and Resources and Needs - Executive Summary
• Upcoming Events

In Memory Of Clyde E. Johnson- September 2004

Nebraska Bird Review

Long-time NOU member Clyde Johnson passed away on May 4, 2003. He is survived by his wife Emma, who now resides in Fredericksburg, Texas.

Clyde and Emma lived in Omaha, where Clyde was employed as an insurance agent. They made numerous birding trips to Central America, Europe and Asia. Few other details of his life are available, which is quite likely just the way Clyde wanted it. Although Clyde was quiet about his personal life, he was well known among NOU members for his contributions of time, enthusiasm and money to the organization.

Clyde and Emma joined the NOV in …

Masthead From Nebraska Bird Review- September 2004

Nebraska Bird Review

The Nebraska Bird Review is published quarterly by the Nebraska Ornithologists' Union, Inc., as its official journal and is sent to members not in arrears of dues. Annual subscription rates (on a calendar-year basis only): $15 in the United States, $18 in Canada and $30 in all other countries, payable in advance. Single copies are $4 each, postpaid, in the United States, $5 in Canada, and $8 elsewhere. Send orders for back issues to Mary Lou Pritchard, NOU Librarian, c/o University of Nebraska State Museum, W-436 Nebraska Hall, Lincoln, NE 685880514.

Menlberships in the NOU (on a calendar-year basis only): …

The 2001 Nebraska Nesting Report, Wayne Mollhoff

Nebraska Bird Review

The spring of 2001 will likely be remembered as the winter that wouldn't end, especially in central and western Nebraska. The last remnants of roadside snowbanks remained in the east until 10 April, but the last blizzard closed down the Panhandle on 21-22 April, and the last appreciable snowfall there was on 19 May. None of these dates represent late records, but are remarkably later than average for the past 10-15 years, and seemed especially burdensome following the long cold winter and the remarkably early spring the previous year.

While I recognize the danger inherent in making generalizations when working …

Summer Field Report, June-July 2004, W. Ross Silcock

Nebraska Bird Review

The coverage of the state by observers who report their sightings, mostly to the listserv NEBirds, is very good relative to their rather low number. Even so, there are parts of the state that are only recently revealing their secrets. Extensive work in the Panhandle in the late 1990s, including mist-netting by Steve Dinsmore, showed that several western species are regular migrants through that part of the state, and similar extensive coverage of waterbirds and shorebirds at Lake McConaughy yielded similar valuable information, following the pioneering efforts there by Dick Rosche. This report contains many references to another interesting area …

The Greater Snow Goose (Chen Caerulescens Atlanticus) In Nebraska, W. Ross Silcock

Nebraska Bird Review

Recently, Mr. William Lemburg of Cairo, Nebraska, in a letter to the Editor of the Nebraska Bird Review, noted the following: "I have a friend who does quite a bit of goose hunting. He remarked to me that during the spring snow goose season, he occasionally shoots an extra-large Snow. Thinking that maybe a few Greater Snows that winter along the Atlantic Coast may end up in the Central Flyway, I asked him to save the head of one if he bagged one the following season. This is about five years ago. The following season he did get one. …

The Official List Of The Birds Of Nebraska: 2003

Nebraska Bird Review

The Official List of the Birds of Nebraska was last published in 1997 (NOU Records Committee 1997). That list included 427 species whose occurrence in Nebraska had been documented to the satisfaction of the NOU Records Committee at that time, following the sequence and nomenclature as outlined by the American Ornithologists' Union (1983, 1985, 1987, 1989, 1991, 1993, 1995, 1997, 1998, 2000, 2002, 2003). The present list includes 447 species based initially on Bray et al. 1986, and subsequently all changes to the Official List of the Birds of Nebraska as determined by the NOU Records Committee (Mollhoff 1987, 1989; …

Nebraska Bird Review Whole Issue September 2004 Volume 72 Number 3

Nebraska Bird Review

Table of Contents

Summer Field Report, June - July 2004
by W. Ross Silcock ...........................78

Birds of Spring Creek Prairie Audubon Center - 1999-2004
by Kevin Poague ...........................94

In Memory of Clyde E. Johnson ...........................98

The 2001 Nebraska Nesting Report by Wayne J. Mollhoff ...........................99

The Greater Snow Goose (Chen caerulescens atlanticus)
in Nebraska by W. Ross Silcock ............................104

The Official List of the Birds of Nebraska: 2003
by Mark A. Brogie ...........................108

Subscription and Organization Information ...........................127

Immunogold Labeling To Enhance Contrast In Optical Coherence Microscopy Of Tissue Engineered Corneal Constructs, Chris B. Raub, Elizabeth J. Orwin, Richard C. Haskell

All HMC Faculty Publications and Research

Our lab has used an optical coherence microscope (OCM) to assess both the structure of tissue-engineered corneal constructs and their transparency. Currently, we are not able to resolve cells versus collagen matrix material in the images produced. We would like to distinguish cells in order to determine if they are viable while growing in culture and also if they are significantly contributing to the light scattering in the tissue. In order to do this, we are currently investigating the use of immunogold labeling. Gold nanoparticles are high scatterers and can create contrast in images. We have conjugated gold nanoparticles to …

Nitrogen Isotope Dynamics Of The Cariaco Basin, Venezuela, Robert C. Thunell, Daniel M. Sigman, Frank E. Muller-Karger, Yrene Astor, Ramon Varela

Marine Science Faculty Publications

We report ¹⁵N/¹⁴N measurements of water column nitrate and ammonium, sinking particles, and sediments from the Cariaco Basin, an anoxic marine basin off the coast of Venezuela. Water column denitrification occurring in the basin has only a very small isotopic imprint on nitrate in the basin because nitrate consumption is nearly complete in the actively denitrifying water near the oxic/anoxic interface (∼275 m). Being free of a large denitrification signal, the δ¹⁵N of shallow thermocline nitrate is ∼3.5‰, significantly lower than the mean deep ocean nitrate δ¹⁵N of 5‰. This may be due …

Vertical Wall Loads In A Model Grain Bin With Non-Axial Internal Inserts, Marek Molenda, Michael D. Montross, Jozef Horabik, Sidney A. Thompson

Biosystems and Agricultural Engineering Faculty Publications

A study was conducted to estimate the degree of load asymmetry in a bin with non-axial internal inserts. Internal inserts in the form of an annulus segment were attached to the wall, and their influence on vertical wall loads during centric filling and discharge in a model bin were measured. Wall and floor loads were measured in a corrugated-wall model grain bin with a diameter of 2.44 m and a height of 7.3 m filled with soft red winter wheat to a depth of 6.7 m (height-to-diameter ratio of 2.75). Tests were conducted with inserts that extended circumferentially 30°, 60°, …

Seasonal Aeration Rates For The Eastern United States Based On Long-Term Weather Patterns, Michael D. Montross, Samuel G. Mcneill, Thomas C. Bridges

Biosystems and Agricultural Engineering Faculty Publications

Most aeration fans are sized to produce a minimum airflow rate of 0.1 m³/min/t (0.1 cfm/bu) in on-farm grain storage structures. At this airflow rate a significant amount of time is required to move a cooling front completely through a bin. The desired grain temperature and prevailing weather conditions will have a significant effect on required fan size. Thirty years of weather data were analyzed for the eastern United States to determine the amount of time available in temperature windows between 0 to 15.C and 0 to 17.C. Contour maps were generated with ArcMap 8.3 for the percentage …

Medical Triggers Are Associated With Better Short- And Long-Term Weight Loss Outcomes, Amy A. Gorin, Suzanne Phelan, James O. Hill, Rena R. Wing

Kinesiology and Public Health

Background. Medical events are often reported as triggers for weight loss, but it is unknown whether medical triggers result in better short- and long-term weight control.
Methods. The relationship between medical triggers and weight loss was examined in the National Weight Control Registry (NWCR), a database of individuals who have lost ≥30 lbs and kept it off for ≥1 year. Recall of weight loss triggers may become difficult over time, thus participants were limited to those reporting weight loss (1) participants with medical triggers (N = 207), (2) participants with nonmedical triggers (N = 539), and (3) participants …

On The Approximation Of The Coriolis Terms In C-Grid Models, Dmitri A. Nechaev, Max Yaremchuk

Faculty Publications

A new representation of the Coriolis terms on the Arakawa C grid is proposed. The approximation dumps the grid-scale noise that arises because of spatial averaging of the Coriolis terms when the grid spacing is larger than the deformation radius. The proposed approximation can also be applied in C-grid schemes with semi-implicit treatment of the Coriolis terms. The new scheme is analyzed in the context of the linear inertial-gravity waves and its advantageous behavior is demonstrated with respect to the conventional technique.

Influence Of Indoor Microclimate And Diet On Survival Of Anopheles Gambiae S.S. (Diptera: Culicidae) In Village House Conditions In Western Kenya, Bernard A. Okech, Louis C. Gouagna, Bart G.J. Knols, Ephantus W. Kabiru, Gerry F. Killeen, John C. Beier, Guiyun Yan, John I. Githure

All Peer-Reviewed Publications

The survival of female Anopheles gambiae s.s. mosquitoes inside two village house types (grass-thatched and iron-roofed) was studied in relation to diet and ambient indoor microclimatic conditions. Two batches of 20-30, 1-day-old laboratory-bred mosquitoes were maintained inside cages in the grass-thatched (n = 2) and iron-roofed (n = 2) houses and fed daily, one group on 10% glucose and the other on human blood. Throughout the experiments, indoor temperature and relative humidity of the houses were recorded, and mortality of mosquitoes monitored daily until all had died. The experiments were replicated thrice. There was no significant variation in the overall …

Cholinergic Modulation Of The Locomotor Network In The Lamprey Spinal Cord, Katharina A. Quinlan, Peter G. Placas, James T. Buchanan

Biological Sciences Faculty Research and Publications

Acetylcholine (ACh) was found here to be a strong modulator of swimming activity in the isolated spinal cord preparation of the adult lamprey (Ichthyomyzon unicuspis). During fictive swimming induced with either D-glutamate or N-methyl-D-aspartate, addition of ACh (200 µM) significantly reduced the cycle period of ventral root bursts to 54%, intersegmental phase lag to 32%, and ventral root burst proportion to 80% of control levels. Effects of ACh were apparent at concentrations as low as 1 µM. Both nicotinic and muscarinic receptors are involved, in that application of either nicotinic or muscarinic agonists alone significantly reduced cycle …

Reticulospinal Neurons Receive Direct Spinobulbar Inputs During Locomotor Activity In Lamprey, James F. Einum, James T. Buchanan

Biological Sciences Faculty Research and Publications

Reticulospinal neurons of the lamprey brain stem receive rhythmic input from the spinal cord during locomotor activity. The goal of the present study was to determine whether such spinal input has a direct component to reticulospinal neurons or depends on brain stem interneurons. To answer this question, an in vitro lamprey brain stem-spinal cord preparation was used with a diffusion barrier placed caudal to the obex, separating the experimental chamber into two baths. Locomotor activity was induced in the spinal cord by perfusion of D-glutamate or N-methyl-DL-aspartate into the spinal cord bath. The brain stem bath was first perfused …

Alfalfa Variety Selection Guidelines, Thomas Griggs

All Current Publications

This fact sheet provides guidelines for selecting the right alfalfa variety and includes preliminary site and end-use consideration, variety selection based on plant traits, and seed quality and treatment considerations.

The Bvg Virulence Control System Regulates Biofilm Formation In Bordetella Bronchiseptica, Yasuhiko Irie, Seema Mattoo, Ming H. Yuk

Biology Faculty Publications

Bordetella species utilize the BvgAS (Bordetella virulence gene) two-component signal transduction system to sense the environment and regulate gene expression among at least three phases: a virulent Bvg⁺ phase, a nonvirulent Bvg⁻ phase, and an intermediate Bvgⁱ phase. Genes expressed in the Bvg⁺ phase encode known virulence factors, including adhesins such as filamentous hemagglutinin (FHA) and fimbriae, as well as toxins such as the bifunctional adenylate cyclase/hemolysin (ACY). Previous studies showed that in the Bvgⁱ phase, FHA and fimbriae continue to be expressed, but ACY expression is significantly downregulated. In this report, we determine …

Pineywoods Native Plant Center, Sept 2004, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.

Technical Assessment Of Natural Resource Management Threats And Options In The Northern Agricultural Region Of Western Australia, Lorinda Hunt, G Patterson, Northern Agricultural Catchments Council (Wa)

Resource management technical reports

Analysis of the risk of soil, land and water degradation within the Northern Agricultural Region of Western Australia. Natural resource issues discussed include: acid groundwater, acid sulfate soils, climate change, dryland salinity, flooding, herbicide resistance, non-wetting (water repellence), nutrient loss and eutrophication, remnant vegetation decline, soil acidity, soil fertility decline, soil structure decline, subsurface compaction, waterlogging, wind erosion and soil contamination.