Life Sciences Commons^™

Inbreeding, Male Diploidy, And Complementary Sex Determination In The Solitary Wasp Euodynerus Foraminatus, Julie Kozaczka Stahlhut

Dissertations

In the insect order Hymenoptera, which includes wasps, bees, and ants, females normally develop from fertilized eggs and are diploid, while males develop from unfertilized eggs and are haploid. Underlying mechanisms of sex determination are more complex and vary among different hymenopteran taxa. One such method is single-locus complementary sex determination, or sl-CSD, in which sex is determined by a single gene locus with many alleles. In species with sl-CSD, diploid individuals that are heterozygous at the sex locus develop as normal females and hemizygous (haploid) individuals as normal males, but diploids homozygous at the sex locus will also develop …

Nonadditive Genetic Effects In Animal Behavior, Lisa M. Meffert, Sara K. Hicks, Jennifer L. Regan

Faculty Publications

Heritabilities, commonly used to predict evolutionary potential, are notoriously low for behaviors. Apart from strong contributions of environmental variance in reducing heritabilities, the additive genetic components can be very low, especially when they are camouflaged by nonadditive genetic effects. We first report the heritabilities of courtship traits in founder‐flush and control populations of the housefly (Musca domestica L.). We estimated the heritability of each male and female display through the regression of the courtships involving daughters and sons (with randomly selected mates) onto the “midparental” courtship values of their parents. Overall, the average heritability was significantly higher for the …

Resistance Of Bt Transgenic Maize To Lesser Cornstalk Borer (Lepidoptera: Pyralidae), Francys M. F. Vilella, Jose M. Waquil, Evaldo F. Vilella, Paulo A. Viana, Paul E. Lynch, John E. Foster

Department of Entomology: Faculty Publications

The lesser cornstalk borer, Elasmopalpus lignosellus (Zeller) (Lepidoptera: Pyralidae), maize causes the symptom known as “dead heart” to maize. The objective of this research was to evaluate if Bt maize would protect seedlings against lesser cornstalk borer damage and whether different temperatures affect the insect-plant interaction.

Two experiments were conducted. Seeds from Pioneer Bt maize hybrids P33G27, P34R07, P34G82, P33A14, and P34D34, expressing the toxin Cry1A(b) were obtained from the market. Seeds from the hybrids Mycogen (experimental) and Garst 8539 expressing, respectively, the toxin Cry1F and Cry9C as well as the control Garst 8539 (non-Bt) were obtained from the seed …

Cold Acclimation Strategy Is Highly Variable Among The Sunfishes (Centrarchidae), Peter J. Lavrentyev, Deidra R. Tschantz, Elizabeth L. Crockett

Biology Faculty Research

We tested the hypothesis that the physiological strategy for acclimating to low body temperature is similar among closely related fish. Largemouth bass (Micropterus salmoides), green sunfish (Lepomis cyanellus), bluegill sunfish (Lepomis macrochirus), black crappie (Pomonix nigromaculatus), and white crappie (Pomonix annularis), all members of the family Centrarchidae, were acclimated to 5° and 25°C. Morphometric variables (total mass, total length, organ masses) and enzyme activities (hexokinase; lactate dehydrogenase; and cytochrome oxidase in heart, liver, and muscle) were measured in 5°C- and 25°C-acclimated fish at 5° and 25°C assay temperatures. Each species displayed a distinct physiological response to cold acclimation that differed …

Historical Changes In Mid-Water Stands Of Common Reed In The Winnebago Pool Lakes, Wisconsin, Anthony O. Gabriel, Leo R. Bodensteiner

All Faculty Scholarship for the College of the Sciences

Despite the tolerance of common reed grass to environmental extremes, mid-water stands in the Winnebago pool lakes of central Wisconsin appear to be diminishing. Formerly occupying shoreline locations, water level manipulations subsequent to dam construction beginning in the 1850's have isolated reed stands off shore. These stands have persisted but casual observations indicate that stand size has been declining. To address this perception we obtained an approximately decadal series of aerial photographs dating back to 1937 for four stands in Lake Poygan. Annual records were available for 1986–94. Using image analysis software, we determined shape and size metrics. Changes in …

Development Of Water-Based Ink System For Braille Printing, Saurabh Varma

Masters Theses

The most commonly used system of writing by and for blind persons is called Braille, named after its French inventor, Louis Braille (1809-1952). Braille is comprised of a rectangular six-dot cell on its end, with up to 63 possible combinations using one or more of the six dots. Braille is the only reliable method of literacy for blind persons, because it enables them to read and write by touch¹.

Currently, Braille is printed by special embossers that serve as a printing press. These embossers create Braille output when it is attached appropriately to computers. The speed of these …

Behavior Of Escherichia Coli O157:H7 In Tomato And Processed Tomato Products, Broderick Eribo, Mogessie Ashenafi

Department of Biology Faculty Publications

The survival of E. coli O157:H7 in acid foods for weeks and its prolonged survival in refrigerated acid foods are well documented. This prompted the study to evaluate survival of E. coli O157:H7 in tomato and processed tomato products. The pH of the various products ranged from 4.2 to 4.8 and some products contained preservatives such as vinegar. Samples were separately inoculated with a mixture of four E. coli O157:H7 strains previously isolated from hamburger meat at lower and higher initial inoculum levels, incubated at 4 and 25 °C and assayed for survival at regular intervals. In fresh whole tomato, …

Shar-Pei Mediates Cell Proliferation Arrest During Imaginal Disc Growth In Drosophila, Madhuri Kango-Singh, Riitta Nolo, Chunyao Tao, Patrik Verstreken, P. Robin Hiesinger, Hugo J. Bellen, Georg Halder

Biology Faculty Publications

During animal development, organ size is determined primarily by the amount of cell proliferation, which must be tightly regulated to ensure the generation of properly proportioned organs. However, little is known about the molecular pathways that direct cells to stop proliferating when an organ has attained its proper size. We have identified mutations in a novel gene, shar-pei, that is required for proper termination of cell proliferation during Drosophila imaginal disc development. Clones of shar-pei mutant cells in imaginal discs produce enlarged tissues containing more cells of normal size. We show that this phenotype is the result of both …

Historical Development Of The Linear Nonthreshold Dose-Response Model As Applied To Radiation, Ronald L. Kathren

The University of New Hampshire Law Review

[Excerpt] "Despite the nearly universal adoption of the linear nonthreshold dose response model (LNT) as the primary basis for radiation protection standards for the past half century, the LNT remains highly controversial and a contentious topic of discussion among health physicists, radiation biologists, and other radiological scientists. Indeed, it has been pointed out that the LNT has assumed the status of a paradigm, synonymous with an ideal, standard, or paragon or perhaps to some, a sacred cow. Reduced to its very basics, the LNT postulates that every increment of ionizing radiation dose, however small, carries with it a commensurate increase …

Arkansas Corn And Grain Sorghum Performance Tests 2002, D. G. Dombek, D. K. Ahrent, R. D. Bond, I. L. Eldridge

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

An Investigation Of Perceived Restrictions On Certified Athletic Trainers Practice, Katy Taake

All-Inclusive List of Electronic Theses and Dissertations

The purpose of this study was to identify the frequency and nature of perceived restrictions by certified athletic trainers (ATCs) and to determine the frequency of perceived restrictions for each practice setting within states that have legislation. This was a qualitative-descriptive study performed with a web-based survey. 10,000 ATCs receiving electronic mail from the National Athletic Trainers' Association (NATA) were invited to participate in this study. 1158 subjects participated in the study, and 724 subjects (295 female and 429 male; age=33.2 ± 8.3yrs) remained after exclusions. A webbased survey was created to evaluate perceived restrictions. Inorder to establish face validity …

Ethnic Differences Of Lower Extremity Kinematics And Ground Reaction Force During Walking, Takuya Okamoto

All-Inclusive List of Electronic Theses and Dissertations

Although gait analysis has been conducted for a long time, there has been little attention paid to ethnic differences in kinematics and kinetics of walking gait. The purposes of this study were: to compare the lower extremity sagittal kinematics and ground reaction forces during walking gait cycle; and to compare the anthropometric dimensions oflower limbs during static standing between Japanese and American Caucasian males. 111 Thirty males from Indiana State University (fifteen from each ethnic group), aged twenty to twenty-nine years, served as subjects for this study. The lower extremity segmental characteristics were measured, using a tape measure, a Vernier …

The Effects Of Post-Cryotherapy Exercise On Ankle Capsular Temperature, Craig A. Jamison

All-Inclusive List of Electronic Theses and Dissertations

Context: Cryokinetics is a common rehabilitative technique. To date, no data describing capsular temperature cycling during cryokinetics have been reported. Objective: To compare ankle capsular temperature before and at the end of each exercise bout during a typical cryokinetics session. Design: A 2 x 10 repeated measure factorial design was used. The independent variables were treatment and bout. The dependent variable for this study capsular temperature of the lateral talo-crural joint. Setting: Data were collected in the Sports Injury Research Laboratory at Indiana State University. Subjects: Nineteen volunteers participated in this study (age= 24.6 + .6 yr; mass= 74.1 + …

Pre-Logging Liana Cutting Reduces Liana Regeneration In Logging Gaps In The Eastern Brazilian Amazon, Jeffrey J. Gerwing, Christopher Uhl

Environmental Science and Management Faculty Publications and Presentations

The cutting of all lianas prior to logging is a reduced-impact logging technique that is predicted to reduce liana proliferation in logging gaps. This study compares liana abundance and species composition in gaps created during conventional and reduced-impact logging in a forest of the eastern Brazilian Amazon. Logging treatments were conducted in side-by-side plots. Shortly following logging, 50-m² plots were located in the approximate centers of four single treefall and four multiple treefall gaps in each logging area. Six years following logging, there were ∼40% fewer climbing lianas in reduced-impact gaps than in conventional logging gaps. In both logging …

Immunocontraception In Japanese Quail Using Mammalian Gnrh Compared To Chicken Gnrh-I And Chicken Gnrh-Ii, Marianna Burks

Open Access Master's Theses (through 2010)

Because of increasing numbers in wildlife, immunocontraceptives are being developed to decrease these animals and other birds. This study was conducted in conjunction with the United States Geological Survey Midcontinent Science Center in Fort Collins, Colorado (USGS), to determine whether mammalian GnRH (m-GnRH) can be used as a potential immunocontraceptive to decrease reproductive fertility in mature Japanese quail.

Eighty male and female Japanese quail were injected with either mGnRH, chicken GnRH-I (c-GnRH-I), chicken GnRH-II (c-GnRH-II), or vehicle (controls). Blood samples were analyzed for estrogen (E2) and testosterone (T) levels, in addition to antibody titer (Aby) development against the corresponding conjugated …

Specialty Tomato Variety Observation Trial For Indiana, 2002, Elizabeth Maynard

Midwest Vegetable Trial Reports

The trial reported here is an extension of the specialty tomato trials conducted in Indiana in 2001. In that trial, 16 varieties were grown in replicated trials in two locations to evaluate their performance and suitability for the restaurant salad market. In this trial, the same 16 varieties plus an additional 17 varieties were grown in unreplicated plots for evaluation of yield and culinary quality.

Specialty Tomato Variety Observation Trial For Indiana, 2002, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

The trial reported here is an extension of the specialty tomato trials conducted in Indiana in 2001. In that trial, 16 varieties were grown in replicated trials in two locations to evaluate their performance and suitability for the restaurant salad market. In this trial, the same 16 varieties plus an additional 17 varieties were grown in unreplicated plots for evaluation of yield and culinary quality.

Ccr4-Bearing T Cells Participate In Autoimmune Diabetes., Soon H. Kim, Mary M. Cleary, Howard S. Fox, Icos Coporation, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Chemokine receptor expression is exquisitely regulated on T cell subsets during the course of their migration to inflammatory sites. In the present study we demonstrate that CCR4 expression marks a pathogenic population of autoimmune T cells. CCR4 was found exclusively on memory CD4(+) T cells during the progression of disease in NOD mice. Cells expressing the CCR4 ligand TARC (thymus- and activation-regulated chemokine) were detected within infiltrated islets from prediabetic mice. Interestingly, neutralization of macrophage-derived chemokine (MDC) with Ab caused a significant reduction of CCR4-positive T cells within the pancreatic infiltrates and inhibited the development of insulitis and diabetes. Furthermore, …

Ocean Zoning For The Gulf Of Maine: A Background Paper, Fara Courtney, Jack Wiggin

Urban Harbors Institute Publications

Coastal and ocean management is a constantly evolving practice. For at least two decades, coastal communities around the world have been experimenting with new ways to control a multitude of activities in their urban and rural harbors. The number and diversity of Marine Protected Areas are growing, with an increasing focus on protecting integrated biological communities, rather than individual species of concern. The United States and Canada are each developing new, comprehensive ocean polices, looking to better integrate management functions at all levels of government.

All of these initiatives – from local to national - reflect some common truths about …

Diagnostic Assays Based On Esterase-Mediated Resistance Mechanisms In Western Corn Rootworms (Coleoptera: Chrysomelidae), Xuguo Zhou, Michael E. Scharf, Srinivas Parimi, Lance J. Meinke, Robert J. Wright, Laurence D. Chandler, Blair D. Siegfried

Department of Entomology: Faculty Publications

Resistance to methyl-parathion among Nebraska western corn rootworm, Diabrotica virgifera virgifera LeConte, populations is associated with increased hydrolytic metabolism of an organophosphate insecticide substrate. An electrophoretic method to identify resistant individuals based on the staining intensity of esterase isozymes on nondenaturing polyacrylamide gels was developed. Three groups of esterases (I, II, and III) were visible on the gels, but only group II esterase isozymes were intensified in resistant populations. A total of 26 and 31 field populations of western corn rootworms from Nebraska (in 1998 and 1999, respectively) were assessed with nondenaturing polyacrylamide gel electrophoresis (PAGE) assays and diagnostic concentration …

Nebraska's Sandhill Crane Populations, Past, Present And Future, Paul A. Johnsgard

Papers in Ornithology

Although the spring concentrations of Sandhill Cranes in Nebraska's Platte Valley are now an avian phenomenon known nationwide, a general appreciation and inventory of this unique concentration of birds has only been attempted in the last few decades. I am often asked how long this largest of all crane concentrations has been occurring in the Platte Valley, and why it has developed only there. Here I will try to summarize the little-known history of this marvelous assemblage, but not dwell on the ecological reasons for it. The latter are now generally well understood to revolve around abundant spring food supplies …

Calliobothrium Spp. (Eucestoda: Tetraphyllidea: Onchobothriidae) In Mustelus Schmitti (Chondrichthyes: Carcharhiniformes) From Argentina And Uruguay, Verónica A. Ivanov, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three species of Calliobothrium inhabit the spiral intestine of Mustelus schmitti in Argentina and Uruguay. Calliobothrium verticillatum australis is redescribed and its taxonomic status modified to species as C. australis. Calliobothrium barbarae n. sp. can be distinguished from all other species of Calliobothrium, which are small bodied, nonlaciniate, and without accessory piece between the bases of axial hook, by worm length, number of segments, cocoon morphology, and hooks shape. Calliobothrium lunae n. sp. is different from other Calliobothrium spp., which are small bodied, nonlaciniate, and have an accessory piece, by the number of segments and testes, hook shape, …

Review Of Parasites And The Behaviour Of Animals By Janice Moore (Oxford University Press, 2002), Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Review of Parasites and the Behaviour of Animals by Janice Moore (Oxford University Press, 2002).

The Birds Of St. Matthew Island, Bering Sea, Kevin Winker, Daniel D. Gibson, Arthur L. Sowls, Brian E. Lawhead, Philip D. Martin, Eric P. Hoberg, Douglas Causey

Harold W. Manter Laboratory of Parasitology: Faculty Publications

St. Matthew Island (608 249 N, 1728 429 W) and its small nearby satellites, Hall Island and Pinnacle Rock, are isolated in the north-central Bering Sea. This infrequently visited location occupies a geographic position with a deep Bering Land Bridge history and is in an area of interdigitation of the Old World, New World, and Beringian avifaunas. It is known for its three Beringian endemics, a bird (McKay’s Bunting, Plectrophenax hyperboreus), a small mammal, and a plant. This level of endemism is striking for a high-latitude island. The only previous summary of the avifauna of St. Matthew Island (Hanna …

Laboratory Analyses Of Water And Shellfish From Coastal Waters And Watershed Of New Hampshire, Jayne S. Finnigan

PREP Reports & Publications

The Department of Health and Human Services-New Hampshire Public Health Laboratories (DHHS-NHPHL) has participated in providing laboratory analyses as part of the National Estuary Program since it’s inception in New Hampshire in 1995. The NHPHL has continued to carry out various actions dealing with the monitoring program as listed in the NH Estuaries Project Management Plan.

The NHPHL implemented selected actions from the NH Estuaries Project Management Plan and Year Six workplan to help address the environmental problems affecting the state’s estuarine systems.

Wildlife Habitat Gis Modeling Study, Society For The Protection Of New Hampshire Forests

PREP Reports & Publications

This technical report is part of a larger study entitled Protecting Wildlife and Significant Habitat in Coastal New Hampshire, an initiative of the Great Bay Resource Protection Partnership (GBRPP), funded by the New Hampshire Estuaries Project. The fieldwork component of the study was implemented by the Audubon Society of New Hampshire (ASNH) and the N.H. Fish & Game Department Non-game and Endangered Wildlife Program (NHF&G) in 2002, in cooperation with The Nature Conservancy of New Hampshire (TNC). This report focuses on the GIS mapping and predictive habitat modeling developed by the Society for the Protection of N.H. Forests (SPNHF) in …

Arkansas Soybean Performance Tests 2002, D. G. Dombek, D. K. Ahrent, R. D. Bond, I. L. Eldridge

Arkansas Agricultural Experiment Station Research Series

Soybean cultivar performance tests are conducted each year in Arkansas by the University of Arkansas Division of Agriculture. The tests provide information to companies developing cultivars and/or marketing seed within the State, and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for soybean producers. The 2002 soybean cultivar performance tests were conducted at the Northeast Research and Extension Center (NEREC) at Keiser, the Cotton Branch Station (CBS) near Marianna, the Rice Research and Extension Center (RREC) near Stuttgart, the Southeast Research and Extension Center - Rohwer Division (SEREC-RD) near Rohwer, the Burton Brothers Farm (BBF) in LaFayette County, …

Arkansas Animal Science Department Report 2002, Zelpha B. Johnson, D. Wayne Kellogg

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Arkansas Corn And Grain Sorghum Performance Tests 2002, D. G. Dombek, D. K. Ahrent, R. D. Bond, I. L. Eldridge

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers. The 2002 corn performance tests contained 59 entries and were conducted at the Northeast Research and Extension Center (NEREC) at Keiser, the Cotton Branch Station (CBS) near Marianna, the Bell Farming Company (BFC) near Des Arc, the Southeast Research and Extension Center - Rohwer Division (SEREC-RD) near Rohwer, and the John Williams Farm (JWF) in Lafayette County. …

Rhoa Kinase And Protein Kinase C Participate In Regulation Of Rabbit Stomach Fundus Smooth Muscle Contraction, Paul H. Ratz, Joel T. Meehl, Thomas J. Eddinger

Biological Sciences Faculty Research and Publications

1. The degree to which the RhoA kinase (ROK) blockers, Y-27632 (1 µM) and HA-1077 (10 µM), and the PKC blocker, GF-109203X (1 µM), reduced force produced by carbachol, a muscarinic receptor agonist, and phenylephrine, an α-adrenoceptor agonist, was examined in rabbit stomach fundus smooth muscle.

2. When examining the effect on cumulative carbachol concentration-response curves (CRCs), ROK and PKC blockers shifted the potency (~EC50) to the right but did not reduce the maximum response.

3. In a single-dose carbachol protocol using moderate (~EC50) and maximum carbachol concentrations, Y-27632 and HA-1077 reduced peak force, but GF-109203X had no effect. By …