Life Sciences Commons^™

Comparison Of The Growth Performance And Mortality In Abant Trout (Salmo Trutta Abanticus Tortonese, 1954) And Rainbow Trout (Oncorhynchus Mykiss Walbaum, 1792) Under Farming Conditions, İrfan Uysal, Ati̇lla Alpbaz

Turkish Journal of Zoology

This experiment was conducted between 15th February 2000 (day 1) and 31st January 2001 (day 350) at the DSI-Gölköy Fish Production Station, Bolu (Turkey). Abant trout larvae obtained from eggs of wild Abant trout broodstocks in Lake Abant and rainbow trout larvae obtained from eggs of the hatchery broodstocks in Gölköy Fish Production Station were used. Trials I and II involved eight groups (four Abant and rainbow trout experimental groups, and four Abant and rainbow trout parallel groups). A total of 1784 larvae was used in both the trials. At the end of the experiment, although the mean wet weight …

Identification Of A Genetic Locus Involved In Ppgpp Metabolism In Escherichia Coli, Roxanne Enid Garza

Theses and Dissertations - UTB/UTPA

Two genes involved in the metabolism of ppGpp are relA and spoT . A relX mutant has altered ppGpp levels, so relX may be a regulator of the RelA or SpoT enzymes. The growth of the relX mutant has been reported to be both temperature and leucine sensitive. It was discovered in this work that this relX mutant is not temperature sensitive, however it is leucine sensitive. The original goal was to study effects of the relX gene on ppGpp metabolism. During the process of mapping the relX gene, it was discovered that the leucine sensitivity was caused by some …

The Use Of Aflp To Detect Genetic Differentiation Within And Among Populations Of Two Prairie Plant Species: Panicum Virgatum And Coreopsis Palmata, Christopher Andrew Hilker

Dissertations and Theses @ UNI

The degree of genetic diversity within any species is crucial to its survival with respect to environmental stresses and its ability to adapt. As native Iowa prairie plant populations continues to diminish, genetic diversity within the state becomes crucially important for restoration, reconstruction, and conservation efforts. This study seeks to determine the degree of genetic variation within native Iowa populations of Panicum virgatum (switchgrass) and Coreopsis palmata (prairie coreopsis, tickseed, prairie tickseed). Plants were obtained directly from the tallgrass prairie, from native seed plantings, and from greenhouse grown cultivated varieties (switchgrass). Amplified Fragment Length Polymorphisms (AFLP) provided genetic fingerprints of …

Evaluating The Prevalence, Content And Readability Of Complementary And Alternative Medicine (Cam) Web Pages On The Internet, Smitha Sagaram, Muhammad Walji, Elmer Bernstam

Faculty, Staff and Student Publications

Complementary and alternative medicine (CAM) use is growing rapidly. As CAM is relatively unregulated, it is important to evaluate the type and availability of CAM information. The goal of this study is to deter-mine the prevalence, content and readability of online CAM information based on searches for arthritis, diabetes and fibromyalgia using four common search engines. Fifty-eight of 599 web pages retrieved by a "condition search" (9.6%) were CAM-oriented. Of 216 CAM pages found by the "condition" and "condition + herbs" searches, 78% were authored by commercial organizations, whose pur-pose involved commerce 69% of the time and 52.3% had no …

Evaluating A Medical Error Taxonomy, Juliana Brixey, Todd R. Johnson, Jiajie Zhang

Faculty, Staff and Student Publications

Healthcare has been slow in using human factors principles to reduce medical errors. The Center for Devices and Radiological Health (CDRH) recognizes that a lack of attention to human factors during product development may lead to errors that have the potential for patient injury, or even death. In response to the need for reducing medication errors, the National Coordinating Council for Medication Errors Reporting and Prevention (NCC MERP) released the NCC MERP taxonomy that provides a standard language for reporting medication errors. This project maps the NCC MERP taxonomy of medication error to MedWatch medical errors involving infusion pumps. Of …

A New Flavor-Coated Sachet Bait For Delivering Oral Rabies Vaccine To Raccoons And Coyotes, Samuel B. Linhart, John C. Wlodkowski, Darrell M. Kavanaugh, Laurie Motes-Kreimeyer, Andrew J. Montoney, Richard B. Chipman, Dennis Slate, Laura L. Bigler, Malcomb G. Fearneyhough

United States Department of Agriculture Wildlife Services: Staff Publications

Research was conducted during 1996–2000 to develop baits for delivering an oral rabies vaccine to raccoons (Procyon lotor) and coyotes (Canis latrans). A bait was sought that: (1) was attractive to the target species, (2) could be distributed by aircraft, (3) was as effective (or more so) than the currently used fish meal polymer bait, and (4) could be produced in large numbers by automated procedures and could be purchased by user groups at substantially lower cost.

Ten field trials were conducted to document raccoons’ bait flavor preferences, evaluate a new vaccine sachet bait coated with …

Discounting And Reciprocity In An Iterated Prisoner’S Dilemma, David W. Stephens, Colleen M. Mclinn, Jeffrey R. Stevens

Jeffrey Stevens Publications

The Iterated Prisoner’s Dilemma (IPD) is a central paradigm in the study of animal cooperation. According to the IPD framework, repeated play (repetition) and reciprocity combine to maintain a cooperative equilibrium. However, experimental studies with animals suggest that cooperative behavior in IPDs is unstable, and some have suggested that strong preferences for immediate benefits (that is, temporal discounting) might explain the fragility of cooperative equilibria. We studied the effects of discounting and strategic reciprocity on cooperation in captive blue jays. Our results demonstrate an interaction between discounting and reciprocity. Blue jays show high stable levels of cooperation in treatments with …

Cellular Tropisms And Co-Receptor Usage Of Hiv-1 Isolates From Vertically Infected Children With Neurological Abnormalities And Rapid Disease Progression, Micheline Mccarthy, Jun He, Denise Auger, Rebeca Geffin, Cecilia Hutto, Charles Wood, Gwendolyn Scott

Nebraska Center for Virology: Faculty Publications

The longitudinal evolution of HIV-1 phenotypes was studied in a cohort of six vertically infected children with early onset and rapid progression of clinical disease. Among 30 viral isolates obtained from peripheral blood, tropisms for both human blood-derived cells (macrophages, T-lymphocytes), and for human neural (brain-derived) cells (microglia, astrocytes) were determined, as was chemokine co-receptor usage. All children harbored from birth macrophage-tropic isolates using the CCR5 co-receptor. Two children later developed T-cell tropic isolates with CXCR4 and CCR3 usage. While all six patients developed neurological abnormalities, only three produced neural cell tropic isolates, which used CCR5. However, early and persistent …

Small Glutamine-Rich Protein/Viral Protein U–Binding Protein Is A Novel Cochaperone That Affects Heat Shock Protein 70 Activity, Peter C. Angeletti, Doriann Walker, Antonito T. Panganiban

Nebraska Center for Virology: Faculty Publications

Molecular chaperone complexes containing heat shock protein (Hsp) 70 and Hsp90 are regulated by cochaperones, including a subclass of regulators, such as Hsp70 interacting protein (Hip), C-terminus of Hsp70 interacting protein (CHIP), and Hsp70-Hsp90 organizing factor (Hop), that contain tetratricopeptide repeats (TPRs), where Hsp70 refers to Hsp70 and its nearly identical constitutive counterpart, Hsc70, together. These proteins interact with the Hsp70 to regulate adenosine triphosphatase (ATPase) and folding activities or to generate the chaperone complex. Here we provide evidence that small glutamine-rich protein/viral protein U–binding protein (SGT/UBP) is a cochaperone that negatively regulates Hsp70. By “Far-Western” and pull-down assays, SGT/UBP …

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention relates to methods and compositions for eliciting an immune response against bovine viral epitopes. The methods comprise combining at least one heat shock protein with at least one bovine viral epitope to form a purified epitope/heat shock protein complex and administration of an immune system stimulating amount of the purified epitope/heat shock protein complex. The compositions comprise, a purified epitope/heat shock protein complex comprising at least one bovine viral epitope complexed with at least one heat shock protein, and a pharmaceutically acceptable carrier, diluent or excipient.

Segregation Of Spermatozoa Within Sperm Storage Tubules Of Fowl And Turkey Hens, L. M. King, J. P. Brillard, M. R. Bakst, A. M. Donoghue

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In avian species, spermatozoa reside in the oviduct for prolonged periods in specialized structures known as sperm storage tubules, but little is known about the relative distribution of spermatozoa in these tubules after successive inseminations by different males. The staining efficacies of various fluorescent dyes for fowl and turkey spermatozoa were evaluated to investigate one proposed mechanism of sperm competition. Hens were then inseminated at different intervals with stained and unstained spermatozoa to observe the spatial distribution of spermatozoa within the storage tubules. Several novel fluorescent lipophilic tracers that successfully stain mammalian spermatozoa either did not stain fowl or turkey …

Repellency Of Ir3535, Kbr3023, Para-Menthane-3,8-Diol, And Deet To Black Salt Marsh Mosquitoes (Diptera: Culicidae) In The Everglades National Park, Donald R. Barnard, Ulrich R. Bernier, Kenneth H. Posey, Rui-De Xue

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

IR3535, KBR3023, para-menthane-3,8-diol (PMD), and deet were evaluated in controlled studies with human subjects (n = 5) for repellency to black salt marsh mosquitoes (Ochlerotatus taeniorhynchus Wiedemann), in the Everglades National Park, FL. In tests of 6-h duration, with an average mosquito biting pressure on exposed forearm skin of 19.5 (±13.7) bites per minute, the mean percent repellencies (SE) for IR3535, KBR3023, PMD, and deet was 88.6 (3.2), 97.5 (1.7), 89.2 (2.9), and 94.8 (2.5), respectively. Mean complete protection times (SE) for IR3535, KBR3023, PMD, and deet were 3.0 (1.0), 5.4 (0.6), 3.8 (1.4), and 5.6 (0.5) h, …

Federally Owned Rangelands: Are There New Grounds For Common Ground?, Mark Rey

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Today, we have recreated the range wars, using less violent, twentyfirst century means, but involving equally passionate views and similarly implacable foes. While some advocates vigorously defend a historic land use, others argue with increasing vehemence for sharp reductions-or even a complete cessation-of such uses, dismissing an entire lifestyle as nihilistic. In the face of such apparently intractable antagonism, some have predicted, with unseemly enthusiasm, the eventual sunset of federal land grazing. Others have advanced the seemingly enlightened idea of buying federal grazing leases as a better approach to improving publicly-owned range habitats.

A Procedural Evaluation Of An Analytic-Deliberative Process: The Columbia River Comprehensive Impact Assessment, Aimee Guglielmo Kinney, Thomas M. Leschine

United States Army Corps of Engineers, Omaha District

The U.S. Department of Energy's Columbia River Comprehensive Impact Assessment (CRCIA) was an ambitious attempt to direct its cleanup of the Hanford Nuclear Reservation toward the most significant risks to the Columbia River resulting from past plutonium production. DOE's approach was uncommonly open, including tribal, regulatory agency, and other Hanford interest group representatives on the board that was to develop the assessment approach. The CRCIA process had attributes of the ``analytic-deliberative'' process for risk assessment recommended by the National Research Council. Nevertheless, differences between the DOE and other participants over what was meant by the term ``comprehensive'' in the group's …

Human Immunodeficiency Virus Type 1 (Hiv-1) Tat Induces Nitric-Oxide Synthase In Human Astroglia, Xiaojuan Liu, Malabendu Jana, Subhajit Dasgupta, Sreenivas Koka, Jun He, Charles Wood, Kalipada Pahan

Nebraska Center for Virology: Faculty Publications

Human immunodeficiency virus type 1 (HIV-1) infection is known to cause neuronal injury and dementia in a significant proportion of patients. However, the mechanism by which HIV-1 mediates its deleterious effects in the brain is poorly defined. The present study was undertaken to investigate the effect of the HIV-1 tat gene on the expression of inducible nitric-oxide synthase (iNOS) in human U373MG astroglial cells and primary astroglia. Expression of the tat gene as RSV- tat but not that of the CAT gene as RSV-CAT in U373MG astroglial cells led to the induction of NO production and the expression of iNOS …

Efficient Infection Of Nicotiana Benthamiana By Tomato Bushy Stunt Virus Is Facilitated By The Coat Protein And Maintained By P19 Through Suppression Of Gene Silencing, Feng Qu, Thomas Jack Morris

Nebraska Center for Virology: Faculty Publications

Tomato bushy stunt virus (TBSV) is one of few RNA plant viruses capable of moving systemically in some hosts in the absence of coat protein (CP). TBSV also encodes another protein (p19) that is not required for systemic movement but functions as a symptom determinant in Nicotiana benthamiana. Here, the role of both CP and p19 in the systemic spread has been reevaluated by utilizing transgenic N. benthamiana plants expressing the movement protein (MP) of Red clover necrotic mosaic virus and chimeric TBSV mutants that express CP of Turnip crinkle virus. Through careful examination of the infection phenotype …

Cypress Spurge, H. Faubert, R. A. Casagrande

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Cypress spurge (Euphorbia cyparissias L.) is a herbaceous European perennial introduced into North America as an ornamental plant in the 1860s (Croizat, 1945). It was widely planted in graveyards and often is called graveyard weed (Muenscher, 1936). The plant escaped cultivation and became established on open ground, particularly in pastures (Stuckey and Pearson, 1973).

Musk Thistle (Nodding Thistle), A. Gassman, L.-T. Kok

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Musk thistle, Carduus nutans L., is an invasive weed that has become widespread in the contiguous states of the United States. It is a highly competitive weed of Eurasian origin that has replaced much of the native vegetation in pastures and disturbed areas.

Slenderflower Thistle (Winged Slender Or Seaside Thistle), A. Gassmann, L.-T. Kok

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Like many other Carduus species, slenderflower thistle, Carduus tenuiflorus Curtis, is associated with pastures, disturbed areas, and vacant lots. Invasion is favored by annual burning of pastures. The thistle protects forage from grazing and is a competitive weed in improved pastures.

Bull Thistle (Spear Thistle), L.-T. Kok, A. Gassmann

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Bull thistle, Cirsium vulgare (Savi) Tenore, is an invasive thistle from Eurasia, found throughout the United States and in Canada from Newfoundland to British Columbia. It is capable of invading fields, pastures, wastelands and along roadsides, but will not survive in cultivated fields.

Plumeless Thistle (Curled Thistle, Bristly Thistle), L.-T. Kok, A. Gassmann

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Plumeless thistle, Carduus acanthoides L., is an introduced Eurasian noxious weed in pastures, rangelands, croplands, and along highways in 19 of the contiguous states in the United States (Frick, 1978). Carduus acanthoides and Carduus nutans L. in the northeastern United States often occupy the same habitats, such as overgrazed pastures and disturbed roadsides, and these species sometimes occur as mixed stands.

Canada Thistle, A. S. Mcclay

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Canada thistle, Cirsium arvense (L.) Scop., is a vigorous, competitive weed that occurs in a wide range of habitats and is difficult to control due to its ability to regrow from its extensive, deep creeping root system (Nadeau and Vanden Born, 1989).

Recommended Terminology And Advances In The Systematics Of The Cyathostominea (Nematoda: Strongyloidea) Of Horses, J. Ralph Lichtenfels, L. M. Gibbons, R. C. Krecek

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Terminology for common names for the Tribe Cyathostominea (cyathostomins), and disease caused by the nematodes (cyathostominosis), were recommended to replace the previously used names cyathostomes and cyathostomosis, which are ambiguous, inaccurate or synonymous, by the Third Internal Workshop on the Systematics of Cyathostominea of Horses, held in Stresa, Italy, 28 August 2001. The progress by this international working group at three workshops is reviewed briefly and a list of publications is provided. Included are an annotated checklist by genus and species of 93 species level names and the recognition of 52 species, redescriptions of seven species, and the description of …

Identification And Characterisation Of A Cdna Sequence Encoding A Glutamic Acid-Rich Protein Specifically Transcribed In Trichinella Spiralis Newborn Larvae And Recognised By Infected Swine Serum, Dante S. Zarlenga, Patricia Boyd, J. Ralph Lichtenfels, Dolores Hill, H. Ray Gamble

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Presently, little is known of the mechanism by which Trichinella penetrates and modulates reprogramming of muscle cells. In light of evidence demonstrating strong protective characteristics of antigens derived from this stage, understanding this process may shed light on potential targets for effective abatement of infection. To this end, a PCR-derived cDNA expression library was constructed using 0.5 mg of total RNA from Trichinella spiralis newborn larvae. The library consisted of >125,000 insert-containing clones. Approximately 40-50 × 10³ clones were screened immunologically using sera from pigs experimentally infected with 7,000 Trichinella L1. Multiple clones reacting positively with the swine infection …

Protostrongylus Stilesi (Nematoda: Protostrongylidae): Ecological Isolation And Putative Host-Switching Between Dall’S Sheep And Muskoxen In A Contact Zone, Eric P. Hoberg, Susan J. Kutz, John Nagy, Emily Jenkins, Brett Elkin, Marsha Branigan, Dorothy Cooley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The occurrence of Protostrongylus stilesi in a population of introduced muskoxen, Ovibos moschatus wardi, on the Arctic Coastal Plain, Yukon Territory (YT) and Northwest Territories (NT), Canada, is consistent with a contemporary colonization event from Dall’s sheep, Ovis dalli dalli, which indicates that host specificity may be ecologically based and contextual for this parasite. Colonization of muskoxen by P. stilesi may be a predictable event in zones of sympatry with Dall’s sheep; exposure to infection may coincide with occupation of winter ranges of Dall’s sheep by muskoxen during the summer season. Disruption of contemporary ecological isolating barriers can …

Foundations For An Integrative Parasitology: Collections, Archives, And Biodiversity Informatics, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Burgeoning awareness about biodiversity emphasizes the fundamental importance of museum collections and the contributions of systematists and taxonomists in documenting the structure and history of the biosphere. An essential role is served by this infrastructure in collecting, preparing, analyzing, and disseminating information about the specimens that represent species, document a range of complex biological associations from symbioses to parasitism, and form the tapestry and the myriad facets of biodiversity (e.g., Wilson, 2000). As parasitologists we can examine how we may contribute to this broader documentation and understanding of global biodiversity, and we can articulate and communicate our role as vital …

Coccidia (Apicomplexa: Eimeriidae) Of The Mammalian Order Chiroptera, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The coccidia are protists (phylum Apicomplexa) that, likely, are both the most abundant (numbers of individual zoites) and most speciose of all the kinds of parasites found in/on mammals. They also are among the least studied and understood, with the exception of those species that cause pathology in domesticated Artiodactyla. In this review, I focus only on the largest family of the phylum, Eimeriidae Minchin, 1903, because its members often are among the most prevalent apicomplexans of mammals, and because there has never been a taxonomic summation for those species that infect Chirpotera. In all published descriptions of bat coccidia, …

Development Of The Muskox Lungworm, Umingmakstrongylus Pallikuukensis (Protostrongylidae), In Gastropods In The Arctic, Susan J. Kutz, Eric P. Hoberg, John Nishi, Lydden Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Development of the muskox protostrongylid lungworm, Umingmakstrongylus pallikuukensis, in its slug intermediate host, Deroceras laeve, was investigated under field conditions in the Arctic. Every two weeks, from 19 June to 28 August 1997, groups of ten experimentally infected slugs were placed in tundra enclosures in a mesic sedge meadow near Kugluktuk, Nunavut, Canada. First-stage larvae (L1) infecting slugs on or before 17 July developed to third-stage larvae (L3) in 4–6 weeks. Intensity of L3 in slugs peaked at 6–8 weeks post infection (PI) and then progressively declined by 10, 12, and 48–50 weeks PI. Abundance of L3 in …

Detection Of Giardia Duodenalis Antigen In Coprolites Using A Commercially Available Enzyme-Linked Immunosorbent Assay, Marcelo Luiz Carvalho Gonçalves, Adauto Araújo, Rosemere Duarte, Joaquim Pereira Da Silva, Karl Reinhard, Françoise Bouchet, Luis Fernando Ferreira

Karl Reinhard Publications

The objective of this experiment was to assess the utility of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for diagnosis of giardiasis in archaeological human remains. The kit, a monoclonal antibody assay, is used to detect the presence of Giardia-specific antigen 65 (GSA65) in human feces. We utilized the assay in ancient fecal material. The material included desiccated feces found in mummies or in archaeological sites, and sediments from latrines. A total of 83 specimens, previously examined microscopically for parasites, were examined. The ELISA detected 3 positive samples, dated to about 1200 AD, 1600 AD, and 1700 AD. …

Multidisciplinary Coprolite Analysis, Karl Reinhard, Dennis R. Danielson, Mark Daniels, Sérgio Augusto De Miranda Chaves

Karl Reinhard Publications

Coprolite analysis, as reviewed by Reinhard and Bryant (1992), contributes unique and detailed information regarding diet and parasitic disease. We present here an analysis of dietary components of coprolites from Bighorn Cave using macroscopic remains, pollen concentrations, and phytoliths. In addition, we analyzed Bighorn Cave coprolites for evidence of parasitic organisms, especially intestinal worms. Such analyses of coprolites have become important methods for reconstructing past dietary and medicinal practices. Pollen concentration and phytolith quantification techniques have recently been developed, but until this report no known attempt has been made to synthesize pollen, macroscopic, and phytolith data from a single coprolite …