Life Sciences Commons^™

Mechanistic Characterization Of The Mitochondrial Type I Dna Topoisomerase And A Study Of Genes Containing Type I Dna Topoisomerase-Related Domains, Jaydee Dones Cabral

Theses and Dissertations in Biomedical Sciences

The mitochondrial type I DNA topoisomerase (mt-topo I) serves an important function in the mitochondrion by relaxing mtDNA supercoils to allow for replication of the mitochondrial genome as well as for gene expression. The mt-topo I's role in essential processes, such as replication and transcription, makes it an ideal candidate as a target for antitumor or antifungal drugs. To gain further insight into mt-topo I mechanism, a cleavage assay and drug inhibition studies were performed. As well, a search for the mt-topo I gene or genes containing type I topoisomerase-related domains was conducted. To characterize the mt-topo I mechanism, the …

Role Of Lipolysis And Lipogenesis In The Development Of Diet-Induced Obesity, Michael John Davies

Theses and Dissertations in Biomedical Sciences

Obesity is an increasingly common public health problem with approximately one-half of the American adult population overweight and one-quarter considered obese. This alarming trend has led researchers to determine potential causative factors of excess weight gain in humans. However, it is difficult to discern whether perturbations that result in obesity are the cause or simply the result of the obese state. Diet-induced obesity is one of the animal models that allow researchers to address temporal issues. Our laboratory utilizes a diet-induced obesity model in which Sprague-Dawley rats are placed on a purified moderately high fat diet and ultimately diverge into …

Degradation In The Brockman River And Ellen Brook Catchments, Western Australia, Brian Lloyd

Natural resources published reports

An eight day survey of degradation in the Ellen Brook and Brockman River catchments revealed several areas of concern. Key concerns are:

Erosion and sedimentation

Various forms of erosion were observed during the survey that would cumulatively have an effect on the quality of both the Ellen Brook and the Brockman Rivers, and ultimately the Swan River. Erosion resulted from many situations including landholder management, subdivision design and infrastructure maintenance. Raising awareness as to consequences of erosion and sedimentation, along with remedial techniques and best management practices is recommended for the various levels of stakeholders.

Eutrophication

Eutrophication has been established …

Bulletin 4418 - Nebru Focus Catchment Report, Agriculture Western Australia

Bulletins 4000 -

Nehru Focus Catchment is located in the upper reaches of the Arrowsmith Catchment. It is roughly bounded by Sunset Road in the north, the north-south Kadathinni Hills to the east, the Three Springs - Eneabba Road to the south and Reed Road in the west. It changes in elevation from 372 metres Australian Height Datum (AHD) at roughly the intersection of Nehru Rd the Kadathinni Hills to the east and 220 metres AHD near the outlet of the catchment into the Arrowsmith River.

The total area of the catchment is just over 7,400 hectares.

One of the prime areas of …

Long-Term Pyrene Exposure Of Grass Shrimp, Palaemonetes Pugio, Affects Molting And Reproduction Of Exposed Males And Offspring Of Exposed Females, Eva Oberdörster, Marius Brouwer, Thea Hoexum-Brouwer, Steve Manning, John A. Mclachlan

Faculty Publications

The objective of this study was to investigate the impact of long-term pyrene exposure on molting and reproduction in the model estuarine invertebrate, the grass shrimp (Palaemonetes pugio). Grass shrimp were exposed to measured concentrations of 5.1, 15.0, and 63.4 ppb (mu g/L) pyrene for 6 weeks, during which time we determined molting and survivorship. At the end of the exposure, we immediately sacrificed some of the shrimp for biomarker (CYP1A and vitellin) analyses. The remaining shrimp were used to analyze fecundity and embryo survivorship during an additional 6 weeks after termination of pyrene exposure. Male shrimp at the highest …

A New Submaximal Bicycle Protocol For The Estimation Of Vo₂Max, Anna R. Bennett

Human Movement Studies & Special Education Theses & Dissertations

Simple, valid, and accurate means for estimating aerobic capacity from submaximal cycle ergometry are needed for use with the general public. Current popular methods such as the ACSM cycle ergometer protocol have been shown to overestimate maximal oxygen uptake (VO₂max) by as much as 26%. In the current study, VO₂max was estimated using a new protocol based on a single 6-min stage designed to elicit 70% HRR. The data was also used to estimate VO₂max by the Astrand method for comparison. Forty-nine healthy males and females, aged 18-44 yr (X = 26 ± …

An Ultrastructural Survey Of The Nuchal Glands Of Rhabdophis Tigrinus (Serpentes: Colubridae), Kathleen A. Roberts

Biological Sciences Theses & Dissertations

Rhabdophis tigrinus, an Asian natricine snake, possesses a series of integumentary glands located in the nuchal skin. Previous studies indicated that the nuchal glands are of mesodermal origin, contain cardiac steroids analogous to bufotoxins found in the parotoid glands of toads, which are included in the snake's diet. The nuchal glands are defensive in function. Preliminary histological studies revealed differences in quality of the gland tissue compared to previous studies, and difference in fixation techniques were suspected. Seven specimens of R. tigrinus were fixed by perfusion using a conservative fixation process standard for electron microscopy. The nuchal gland series …

Comparative Systematics Of Subterranean Amphipod Crustaceans In The Families Crangonyctidae And Bogidiellidae, Stefan Koenemann

Biological Sciences Theses & Dissertations

The research project presented in this doctoral dissertation deals with the systematics of two different taxonomic groups of subterranean amphipods (Crustacea). Therefore, rather than being a single project, this study is divided into several sub-projects. Altogether, the chapters for the sub-projects are composed of five publications. An introductory chapter and a summarizing discussion are added to provide a structural unit for the collection of papers and to compare the results of the individual projects.

The thesis investigates the systematics of the amphipod families Bogidiellidae Hertzog, 1936, and Crangonyctidae Bousfield, 1973. Based on descriptive taxonomy according to modern standards, revisions are …

Validation Of Passive Air Sampling Monitors Onboard United States Navy Submarines, Larry A. Mcfarland

Community & Environmental Health Theses & Dissertations

An operating submarine creates a unique air quality mixture of compounds that result from a combination of human metabolism, construction materials, materials brought onboard and compounds created through the interaction of ship systems. A comprehensive study of submarine atmospheres is ongoing during deployments of U.S. Navy nuclear submarines. As part of the overall effort, a paired air sampling comparison field validation was conducted to compare the air sampling effectiveness of passive diffusive monitors compared to more traditional active air sampling methods when sampling for acrolein, formaldehyde and ozone in the enclosed submarine atmosphere. Acrolein monitors containing 2-hydroxymethylpiperidine (HMP) impregnated glass …

Investigations On The Use Of Ion Mobility Spectrometry For Clinical Chemistry Applications, Henri Parson Patten

Theses and Dissertations in Biomedical Sciences

The major objective of this research is to examine ion mobility spectrometry as a rapid screening tool for specific application to clinical chemistry research and laboratory use. Methodology was developed for target analytes representing several classes of physiologically active substances, including anesthetics, illicit drugs, and their metabolites. The IMS characteristics of animal tissues and other compounds such as amino acids and proteins were determined. Quality assurance and control procedures were developed for specific quality data objectives. Criteria were established relating to use of IMS for assessing the precision and accuracy of data, qualitative screening, and semi-quantitative analyses.

It was found …

Ampelopsis Cordata Michx., John E. Ebinger

Specimens by Name

No abstract provided.

2000 Semi-Annual Report, Various Authors

Annual Reports

No abstract provided.

Phyla Lanceolata (Michx.) Greene, John E. Ebinger

Specimens by Name

No abstract provided.

Agricultural Research Division 114th Annual Report 2000

Agricultural Research Division: News and Annual Reports

It is a pleasure to provide you with a copy of the 114th Annual Report of the University of Nebraska Agricultural Research Division (ARD). This report is our opportunity to inform you about our research programs and accomplishments and to allow us to assess the progress and effectiveness of our collective efforts to develop new knowledge. After examining this report, we hope that you will agree with our assessment that ARD research has provided new technology and knowledge for Nebraskans that will improve the profitability of their enterprises, enhance environmental quality, and improve their quality of life.

This report provides …

Increased Levels Of Multiple Forms Of Dihydrofolate Reductase In Peripheral Blood Leucocytes Of Cancer Patients Receiving Haematopoietic Colony-Stimulating Factors: Interim Analysis, M P. Iqbal, I A. Burney, F Sultana, N Mehboobali, T Siddiqui

Department of Biological & Biomedical Sciences

The precise mechanism whereby granulocytes proliferate when haematopoietic colony stimulating factors (CSFs) are used in neutropenic cancer patients is poorly understood. The purpose of this study was to investigate whether these cytokines bring about leucocyte proliferation by increasing the levels of multiple forms of dihydrofolate reductase (DHFR). Blood samples were collected from 36 cancer patients (25 males and 11 females) with chemotherapy-induced neutropenia. One sample of blood from each patient was obtained before therapy either with CSF, such as granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF) or with placebo, and another one at the time of …

Responding To Financial Stress, Larry L. Bitney

Cornhusker Economics

Drought, low grain prices and high fuel prices are on the minds of many Nebraska farm families this summer. The impact of these events will of course be different by area of the state, by enterprise mix on each farm and the financial position of the business coming into this year. Unless managed properly, these events are likely to lead to financial difficulty this fall or next spring.

Changes In Soil Microbial Community Structure With Tillage Under Long-Term Wheat-Fallow Management, Rhae A. Drijber, John W. Doran, Anne M. Parkhurst, D. J. Lyon

Department of Agronomy and Horticulture: Faculty Publications

Fatty acid methyl esters (FAMEs) were used to `fingerprint' soil microbial communities that evolved during 25 years of wheat-fallow cropping following native mixed prairie sod at Sidney, Nebraska, USA. Total ester-linked FAMEs (EL-FAMEs) and phospholipid-linked FAMEs (PL-FAMEs) were compared for their ability to discriminate between plots remaining in sod and those cropped to wheat or left fallow under no-till, sub-till or plow management. Cropped plots were higher in microbial biomass than their fallowed counterparts, and did not differ significantly with tillage for the 0±15 cm depth. Under fallow, microbial biomass was greatest in no-till and least in plow. Both cluster …

Fraxinus Pennsylvanica Marsh., John E. E. Ebinger

Specimens by Name

No abstract provided.

Vitis Vulpina L., John E. Ebinger

Specimens by Name

No abstract provided.

Parthenocissus Quinquefolia (L.) Planch., John E. Ebinger

Specimens by Name

No abstract provided.

Parthenocissus Quinquefolia (L.) Planch., John E. Ebinger

Specimens by Name

No abstract provided.

Simulating Inbred-Maize Yields With Ceres-Im, Daniel P. Rasse, Joe T. Ritchie, Wally Wilhelm, Jun Wei, Edward C. Martin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

CERES-Maize, which was designed for simulation of hybrid maize (Zea mays L.), cannot be applied directly to seed-producing inbred maize because of specific field operations and physiological traits of inbred maize plants. We developed CERES-IM, a modified version of CERES-Maize 3.0 that accommodates these inbred-specific operations and traits, using a set of phenological measurements conducted in Nebraska (NE), and further tested this model with a set of field data from Michigan (MI). Detasseling (i.e., removal of the tassels from the female plants) was conducted prior to silking. Male rows were removed approximately 10 d following 75% silking. The thermal …

Who Is Buying Nebraska Farmland?, Bruce B. Johnson, David Drozd

Cornhusker Economics

As part of our ongoing monitoring and analysis of Nebraska抯 agricultural land market, we have tracked for several years the patterns and trends of actual real estate transactions. Each year, our reporters from around the state provide us with detailed information on about 500 transactions which have occurred during the previous 12 months. They report on sales which they deem as armslength and typical for their locality. Given that only about three percent of the state's agricultural land base changes ownership each year, the tracts reported in our survey represent about 10 percent of the total annual transactions? a size …

Lysimachia Lanceolata Walter, John E. Ebinger

Specimens by Name

No abstract provided.

Viola Pubescens Aiton, John E. Ebinger

Specimens by Name

No abstract provided.

Near-Surface Phytoplankton Distribution In The Western Intra-Americas Sea: The Influence Of El Niño And Weather Events, Nelson M. Gonzalez, Frank E. Muller-Karger, Sergio Cerdeira Estrada, Roberto Perez De Los Reyes, Ivan Victora Del Rio, Pedro Cardenas Perez, Ida Mitrani Arenal

Marine Science Faculty Publications

The space-rime variation of phytoplankton pigments in the western Intra-Americas Sea (IAS), in the vicinity of the island of Cuba, is examined using digital images obtained with the Coastal Zone Color Scanner sensor flown aboard the Nimbus 7 satellite from 1978 to 1986. The results are compared to historical in situ hydrographic observations. A marked seasonality in pigment concentration was observed in waters around Cuba, with an average of 0.07 mg m(-3) in summer (April-September) and 0.13 mg m-3 during winter (October-March). The range of variation in pigment concentration was larger in the Gulf of Mexico relative to the western …

Modified Phase Contrast Microscopy And Its Applications To The Observation Of Marine Biological Objects, Ming Chang Shih, Jiang-Shiou Hwang

Journal of Marine Science and Technology–Taiwan

We demonstrate the imaging technique for the study of marine micro-biota (e.g. size ranged from um to cm) by modifying the Fourier optics with a near IR illuminating source to improve the contrast of the high spatial frequency images and to reveal the micro-structure of the observed biological object. We also present the application of this system to the study of swimming behavior of the Artemia in free space, and the detailed structure of the spines and podia of the sea urchin. The imaging system is consisted of a collimated laser beam, a high pass Fourier optics transform system, a …

Preharvest Soybean Marketing Strategies, George H. Pfeiffer, Roger K. Wilson, Jim Kendrick

Cornhusker Economics

Soybean producers who decide to use the futures markets to price their crop face a number of important decisions. Among the choices they face are whether to use futures or options on futures, when should positions be established and liquidated, which futures months are most appropriate and what particular marketing strategies or combinations of strategies to employ. Ongoing research in Nebraska on soybean marketing strategies from the early 80's through the present has revealed evidence that relatively simple marketing strategies employing futures markets can be profitable for producers, and that the most profitable strategies have been relatively consistent over time. …

Meeting Minutes, June 14th, 2000, National Smokejumper Association Board Of Directors

National Smokejumper Association Meeting Minutes

Agenda: Meeting was called to order.; Minutes.; NEW BUSINESS; proposal to develop and sell a yearbook to smokejumpers.; Membership Report.; Treasurer's Report.; Website Report.; Smokejumper Magazine.; Merchandising Report.; Reunion Committee Report.; Bylaws Committee Report.; Video Committee report.; Executive Committee, once a year, review all outstanding contracts.; History Committee report.; NEW BUSINESS; An association Loan Policy was discussed.; Long Range Planning report.; Election results.; Number of Board members was discussed.; the death of an Alaska Jumper, Dave Liston.; Work Plan for 2000-2001.; Meeting adjourned.;

Method And Apparatus For Controlling Acoustic Signal Bandwidth In An Ultrasonic Diagnostic Imaging System, Jeffrey R. Resnick, Gregory R. Bashford

Biomedical Imaging and Biosignal Analysis Laboratory

An ultrasonic imaging system includes a receive beamformer that generates analog receive signals and a scan converter. A receive signal processing path interconnects the receive beamformer and the scan converter, and this processing path included both an A/D converter characterized by a selectable sampling rate and at least one filter characterized by at least one filter parameter. The filter parameter is selected as a function of the sampling rate to provide enhanced image quality.