Life Sciences Commons^™

Production Of Polyhydroaxyalkanoate (Pha) From Palm Oil By Pseudomonas Oleovorans And Indigenous Bacterial Isolate, Pseudomonas Sp., Zazali Alias

Student Works (2000-2009)

In this study, the yield of biomass and polyhydroxyalkanoate  (PHA) of Pseudomonas oleovorans grown in batch and fed-batch cultivations were compared. When oleic acid (QA) (0.5% w/v) was used as carbon source, a significant increase in biomass.and PHA content were observed through fedbatch cultivation. The biomass and PHA content obtained from batch cultivation were 1.45±0.19 g/I and 2.50±0.77 % cell dry weight (CDW) respectively, while in the fed-batch cultivation, the biomass and PHA content increased significantly to 3.35±0.78 g/I and 32.72±0.30 %CDW respectively.  When saponified palm olein (SPO) (0.5% w/v) was used as a carbon source, the batch cultivation yielded …

Subcloning, Characterization And Sequencing Of The Streptomycin Resistance Gene Of A Multiple Antibiotic Resistance Transposon From Salmonella Typhi, Ramesvari Pararajasingam Pillai

Student Works (2000-2009)

Salmonella typhi S8 is resistant to ampicillin (Ac), chloramphenicol (Cm), cotrimoxazole (Ctm), streptomycin (Sm), and tetracycline (Tc). The Ac, Cm, Ctm. and Sm resistance traits have been shown to be mediated by a multiple antibiotic resistance transposon located on a large conjugative plasmid. Transposition of the transposon encoding Ac^R (ampicillin resistance), Cm^R Ctm^R and Sm^R designated TnX8, was done by Wong (1999) to a recipient replicon, plasmid pUB307. The Ac^R Cm^R, Ctm^R and Sm^R genes were subcloned into plasmid pKan by digesting the recombinant plasmid, des ignated pCL8 (pUB307::TnX8), with San. This subcloning strategy generated different recombinant pKans that harbour …

Method Development And Validation For Drug Analysis By Hplc Gc-Ms, Yann Jen Goh

Student Works (2000-2009)

Enalapril and its metabolite Enalaprilat were determined by gas chromatography - mass spectrometry (GC - MS) and high - performance liquid chromatography (HPLC). For HPLC, the detection limit of enalapril and enalaprilat was 10n g/ml in methanol, whereas for the GC - MS, the detection limit was 6.25ng/ml in plasma. These methods were compared and GC - MS was selected for routine analysis. Enalapril and Enalaprilat in plasma were extracted and cleaned up by using Sep - Pak C 18. Enalapril and Enalaprilat were detected after reaction with diazomethane and were identified by gas chromatography - mass spectrometry as methyl …

Method Development By Hplc For Separation Of Various Hydrocarbon Groups In Coal Samples From Batu Arang, Selangor, Yong Chee Hui Audrey

Student Works (2000-2009)

A preliminary work involving method development for normal stationary phase (silica) pennits the high-performance liquid chromatographic separation or aliphatic and polyaromatic hydrocarbon (PAHs) classes. The Soxhlet extraction method was applied on the coal rock samples and normal phase (silica) short column clean-up separation from highly polymeric material in the coal extract. Method developed on analytical and preparative normal phase silica HPLC columns were to fractionate coal rock extracts, isocratic elution of hexane: ethyl acetate (95 :5, V:V) was found to be quite a good mobile phase. An alternative fractionation method, preparative thin layer chromatography (TLC), was also investigated to separate …

Use Of Oak Hammocks By Neotropical Migrant Songbirds During Stopover In Coastal South Carolina, Scott Glen Somershoe

College of Graduate Studies: Theses & Dissertations (1964–2006)

Many species of Neotropical migrant songbirds have been reported to be declining in the last few decades, and the loss and fragmentation of stopover habitat has been implicated as a potential cause of these population declines. To manage properly a fragmented forest landscape for migrating songbirds information is needed on how the size and the habitat of forest fragments influence their use by migrants during stopover. Therefore, I conducted censuses of Neotropical migrant birds in eight oak hammocks of varying size (0.32-3.08 ha) at the Savannah National Wildlife Refuge, Jasper County, South Carolina. Censuses were conducted at 0600-1030 h during …

Isolation And Identification Of Sterols In Palm Oil And Their Analysis By Gc Adn Gc-Ms, Ai Chen Han

Student Works (2000-2009)

The unsaponifiablc matters were separated from crude palm oil, crude palm kernel oil, and crude palm fibre oil. The sterol fractions were isolated by preparative thin layer chromatography and were then analysed by gas chromatography and combined gas chromatography-mass spectrometry. Qualitative separations of sterols are illustrated. Tables are presented showing the sterol composition determined in the oi ls, values obtained for individual contents are in broad agreement with other published results There were no major differences between crude palm oil, crude palm kernel oil and crude palm fibre oil with respect to their sterols oomposition. P-sitosterol was found as the …

Kelimpahan Dan Biojisim Makrofauna Di Hutan Bakau Dan Kawasan Bersebelahan, Zainal Abidin Zakaria

Student Works (2000-2009)

A study of abundance and biomass of the macrofauna in the mangroves in Sementa (Selangor) and Kuala Sepetang (Perak) was carried out monthly from September 1993 to December 1994 and from January 1994 to December 1994 . Two sites, a forested and a cleared area (deforested) were selected in each location for the study. Sampling for macrofauna was done by randomly selecting a spot and then pressing an aluminium quadrate of 40 cm x 40 cm into the sediment up to a depth of 20 cm. Eight quadrates were sampled on each occasion. The macrofauna community consisted of the following …

Kajian Kultur Tisu Tumbuhan Zinnia Elegans Jacq. Dan Rosa Hybrida Linn. Var. Christian Dior, Rashidi Othman

Student Works (2000-2009)

From the first experiment, it was found 1ha1 the primary root elongation of Z. elegans was optimum on the fifth day with a value of 40.82 ± 15.39 mm Thus, the root segment with an elongation in the range of25.43 to 56.21 mm has been adopted as the standard length for the m VI VO and m vt/fo cytological studies. In vivo cytological studies on Z. elegans revealed that the mitotic index (MI) measured was 13.47 ± 0.46%, the chromosome number observed was 2n = 23, the average cell and nuclear area values were 113.55 ± 4.44 μm1 and 40.58 …

Secondary School Students' Attitudes Toward Physical Activity, Mai Keok Low

Student Works (2000-2009)

The purpose of this study is to investigate the attitude of Malaysian secondary school students toward physical activity, age 16, in Senior Methodist Girls ' School, Kuala Lumpur. The dependent variable of this study is attitude in terms of the six perceived sub-domains of physical activity. The sub-domains are: physical activity as an aesthetic experience physical activity as catharsis; physical activity as a pursuit of vertigo physical activity for health and fitness; physical activity as a social experience and physical activity as an ascetic experience. The independent variables are academic fields of study (Arts and Science) and athletic experience of …

Ontogeny, Reproduction, And Population Dynamics Of Stoplephorus Baganensis And Thryssa Kammalensis (Pisces : Engraulididae) In A Malaysian Estuary Sarpedonti, Valerie, Valerie Sarpedonti

Student Works (2000-2009)

Larval ontogeny of Stolephorus baganem:;is and Thryssa kammalensis, comprises three successive phases characterized by: (I) development of swimming appendages, (2) changes in allometric growth of various body parts, and (3) increase in body depth, as well as eye diameter, in the case of S. baganensis. Both anchovy species are multiple spawners, spawning all year round but varying in intensity. Mass spawnings of S bagane11sis start in the first (summer) inter-monsoon period and last for four months. This period is associated with changes in the wind field and increased rainfall, dissolved oxygen and food availability. The presence of mature adults during …

Respiration And Heart Rate At The Surface Between Dives In Northern Elephant Seals, Burney J. Le Boeuf, Daniel E. E. Crocker, Jason Gedamke, J. Grayson, Paul M. Webb, Susanna B. Blackwell, Daniel P. Costa

Arts & Sciences Faculty Publications

All underwater activities of diving mammals are constrained by the need for surface gas exchange. Our aim was to measure respiratory rate (fb) and heart rate (fh) at the surface between dives in free-ranging northern elephant seals Mirounga angustirostris. We recorded fb and fh acoustically in six translocated juveniles, 1.8-2. 4 years old, and three migrating adult males from the rookery at Ano Nuevo, California, USA. To each seal, we attached a diving instrument to record the diving pattern, a satellite tag to track movements and location, a digital audio tape recorder or acoustic datalogger with an external hydrophone to …

Application Of A Polymerase Chain Reaction-Elisa To Detect Wuchereria Bancrofti In Pools Of Wild-Caught Anopheles Punctualatus In A Filariasis Control Area In Papua New Guinea, Moses J. Bockarie, Peter U. Fischer, Steven A. Williams, Peter A. Zimmerman, Lysaght Griffin, Michael P. Alpers, James W. Kazura

Biological Sciences: Faculty Publications

Chemotherapy-based eradication programs are aimed at stopping transmission of Wuchereria bancroftiby its obligatory mosquito vector. This study compares one year post-treatment W. bancrofti infection rates of Anopheles punctulatus, the main vector of lymphatic filariasis in Papua New Guinea, using traditional dissection techniques and a polymerase chain reaction (PCR)-based ELISA of a parasite-specific Ssp I repeat. A total of 633 mosquitoes in 35 batches were dissected. Six batches contained W. bancrofti-infected mosquitoes, giving a minimum infection rate of 0.9%. This value was not different than the actual infection rate, which was 9 (1.4%) of 633 mosquitoes (P� 0.48). The DNA was …

The Abundance And Distribution Of Arthropods From Sandstone Outcrops In Coffee County, Georgia, F. Michael Mcaloon

College of Graduate Studies: Theses & Dissertations (1964–2006)

Broxton Rocks Nature Preserve, a 1,233-hectare preserve containing late Pleistocene sandstone (Altamaha grit) outcrops of various sizes and topographies in a conifer-dominated loblolly pine - hardwood forest, represents a unique habitat to the coastal plain of Georgia. Using modified pitfall traps, I sampled arthropods in four subhabitats (bare rock, lichens, ecotone, wiregrass) at Broxton Rocks between January and November 1999. A total of 312,006 arthropods belonging to four classes (Arachnida, Diplopoda, Chilopoda, Insecta) represented by 24 orders was trapped. The numbers of arthropods varied among habitat types, and over the collection dates (P

The Effects Of Low Ph On Sunfish Assemblages In Southeast Georgia, William Boyd Tate

College of Graduate Studies: Theses & Dissertations (1964–2006)

No abstract provided.

Cloning And Sequencing Of Sodium-Hydrogen Exchanger In The Gills Of The Long-Horned Sculpin (Myoxocephalus Octodecimspinosus), Danielle Lee Gunning

College of Graduate Studies: Theses & Dissertations (1964–2006)

No abstract provided.

Effects Of Height And Vegetation On Success Of Bird Nests In Maritime Forests, Sharon M. Defalco

College of Graduate Studies: Theses & Dissertations (1964–2006)

Nest predation is an important source of mortality in songbirds and may contribute to declines in Neotropical migrants. I used artificial nests baited with fresh Japanese Quail and Zebra Finch eggs in conjunction with observations of natural nests to quantity the ettects ol nest height and vegetation on nest predation on St. Catherine's Island, a barrier island in southeast Georgia. Because of intense browsing by white-tailed deer. I predicted that lower, more exposed nests would be least successful. Artificial nests (n = 389) were placed in the field, and natural nests (n = 49) were observed, durintz April and May …

The Effects Of Forest Fragment Size, Isolation, And Microhabitat Variables On Nest Box Use By Southern Flying Squirrels (Glaucomys Volans) In Southern Illinois, Catherine Wong, Eric K. Bollinger, Thomas A. Nelson

Faculty Publications

We studied the effects of forest fragmentation on southern flying squirrels (Glaucomys volans) in southern Illinois, and examined factors influencing nest box use. Ten nest boxes were placed in each of 30 oak-hickory forest fragments. Nest boxes were checked monthly after installation. We measured habitat variables that described the nest box tree, the microhabitat surrounding the nest box tree, and landscape level characteristics of the fragments (isolation and area). Overall, 78% of the 300 nest boxes were used. Glaucomys volans was captured in 24 of the 30 forest fragments, and evidence of squirrel use was found in 4 additional fragments. …

A Quantitative And Qualitative Study On The Benthic Algal Floraof Palandöken Pond, Hasan Gürbüz

Turkish Journal of Biology

In this study, the composition and density of the benthic algae and seasonal variations of the benthic algae of Palandöken Pond were determined between September 1990 and September 1992. Samples were collected from two previously selected sampling sites. It was found that Bacillariophyta was the dominant benthic algal group in this pond. During our investigation a total of 160 taxa were identified and 135 them were composed of epipelic algae. The percentages of algal groups were as follows: Bacillariophyta 57%, Cholorphyta 11% and Euglenophyta5%. It was found that there were plenty of epipelic algal species living together with epiphytic and …

Investigation Of Selected Industrially Important Enzymatic Activities Of Turkish Agricultural Mycoflora-I: Amylase, Lipase, Protease, Şemi̇nur Topal, Ceyda Pembeci̇, Mehli̇ka Borcakli, Mehmet Batum, Özlem Çelti̇k

Turkish Journal of Biology

In this context, fungi being a rich source in from metabolites point of view, their utilization possibilities in various area are investigated. Primary metabolites of the fungi representing Turkish agricultural mycoflora which belongs to our culture collections are determined and their importance with respect to industry will be evaluated. It is assumed that enzymes such as, protease, amylase, lipase, cellulase, xylanase, b-glucanase, pectinase of these moulds represent a potential source for food industry. In this context, qualitative screening three of these enzymes producing abilities of relevant moulds are carried out. Methods used for enzyme determination cover, "Opacity Capacities in Deep …

The Population Structure And Growth Properties Of Chalcalburnusmossulensis (Heckel, 1843) Caught From Askale Region Of River Karasu, Mustafa Türkmen, İhsan Akyurt

Turkish Journal of Biology

In this study, the population structure and growth properties of Chalcalburnus mossulensis (Heckel, 1843) caught from Askale Region of River Karasu betwen February 1996 and January 1997 were examined. The age composition of population varied between I and VI ages, and the most abundant age group was the third age group (42.13%). The population was composed of 51.74% females and 48.26% males. The fork length and the body weights of males and females varied between 8.9-18.1 cm, 3.6-67.9 g and 8.5-18.5 cm, 5.1-79.5 g respectively. The specific and relative growth for length and weight were maximum in the second age. …

Increase Of The \Alpha-Amylase Yield By Some Bacillusstrains, Eli̇f Sarikaya, Veli̇tti̇n Gürgün

Turkish Journal of Biology

In order to obtain high amounts of \alpha-amylase enzyme from Bacillus bacteria a new medium was developed. In this medium, three strains of Bacillus bacteria were grown in the presence of different carbon, nitrogen and metal ions. The highest \alpha-amylase yield was obtained by addition of Na-citrate, strach and sucrose for the strains of B. subtilis, B. amyloliquefaciens I and B. amyloliquefaciens II, respectively. As a nitrogen source, yeast extract was found to be effective on enzyme yield for B. subtilis and B. amyloliquefaciens II, while ammonium sulfate was found to be effective for B. amyloliquefaciens I. None of the …

Study Of Interphases Betweensubunits Of Human Serum Albumin By Tritiumlabelling Method, Elimchan S. Dzhafarov

Turkish Journal of Biology

For the purpose of obtaining information about interphases between subunits of human serum albumin (HSA), a new tritium labelling method was adopted. The existence of linear dependence between accessible surface of amino acid residues and their specific radio-activity enabled to obtain information about qualitattive and quantitative amino acid composition of surface layer of protein globule and its large fragments. It is shown that the fragments surface characterized by higher accessibility of hydrophobic amino acid residues such as Ala, Val, Leu, Ile, Pro and Phe. Moreover Lys, Asx and Glx demonstrate high accessibility also. It is supposed that the spatial HSA …

Distributions And Possible Roles Of Laminin, Fibronectin And Theirreceptor Subunits Integrin \Beta4 And \Alpha5 In Remodelling Of Extracellularmatrix During Decidualization In Rats, Ümi̇t Ali̇ Kayişli, Mevlüt Asar, Ramazan Demi̇r

Turkish Journal of Biology

In the endometrium preparing for pregnancy, the stromal matrix components of the cells and their products begin structural differentiation due to endometrial receptivity. According to the hypothesis that 'extracellular matrix (ECM) proteins and adhesion molecules take roles in the structural differentiation as a result of endometrial decidualization', our aim was to determine the roles of ECM molecules laminin, fibronectin and adhesion proteins integrin \alpha5 and \beta4 in the decidulization and implantation by examining the emergence and changing distribution of these proteins. In this study, five experimental groups, consisting of 40 pregnant animals on pregnancy days 4-8, were used. Paraffin tissue …

The Effect Of Soy Flour On Alcohol And Cell Concentration In Alcoholfermentation Of Molasses, Tuncay Ari, Sedat Dönmez

Turkish Journal of Biology

The effects of soy flour on alcohol fermentation of molasses by different yeast strains were investigated. Six strains of Saccharomyces cerevisiae and one Saccharomyces bayanus were used. It was found that soy flour affected fermentation time, alcohol concentration and total cell count in all strains. But the degree of this effect was different among strains. The maximum ethanol concentration were 63.9 g/l in Saccharomyces cerevisiae N3 strains. The maximum theoritical yield, however was 91.6% in Saccharomyces cerevisiae W4 strains. Compared with the control, the largest increment in ethanol concentration was in Saccharomyces bayanus 1A as 12.2%. Furthermore, addition of soy …

Effects Of Exogenous Spermine On Seed Germination And Early Growthof Sunflower Seeds Under Salinity Stress, Fatma Mutlu, Suna Boncuk

Turkish Journal of Biology

The effects of various concentrations (0.01, 1, 2mM) of spermine (Spm) on germination and some early growth parameters of sunflower ( Helianthus annuus L. cv. Santafe) seeds were investigated in media containing different concentrations of NaCl (50, 100, 200mM). Regardless of its concentrations, application of Spm under non-saline conditions remained ineffective on germination percentage as well as on the early growth parameters studied (length of radicle, fresh and dry weights). The salt, depending on its concentration, inhibited or delayed the germination to a great extent. The growth parameters studied in the early growth period were also adversely affected by salt …

Transmission Of The Embryonic Stem Cell (Esc)Genome To Offspring And Sexual Development Inchimeric Mice From A Male Esc Line, Sezen Arat

Turkish Journal of Biology

This study was performed to determine the capability of embryonic stem (ES) cells at late passage number to form functional germ cells and to orientate the sexual development of chimeric mice produced from ES cell injection into embryos. When an R1 ES cell line was routinely cultured on mitotically inactivated feeder cells in culture medium supplemented with leukemia inhibitory factor (LIF), the colonies maintained an undifferentiated ideal ES cell morphology. When the ES cells were injected into 45 eight-cell embryos, 38 embryos (84%) combined with ES cells. Twenty-eight percent of the embryos transferred to recipients developed to term. Thirty percent …

Germin, An Oxalate Oxidase, Has A Function In Manyaspects Of Plant Life, Mahmut Çalişkan

Turkish Journal of Biology

Germin, a molecular marker of wheat embryo germination, is a remarkably protease-resistant, apoplastic, homopentameric glycoprotein. Different forms of germin found to be related to various aspects of plant development have been discovered. Germins were determined in cereals, dicotyledonous angiosperms, gymnosperms and in a prosit, all these proteins are together called germin-like proteins due to their DNA sequence homology. Cereal germins have oxalate oxidase activity, an activity which generates hydrogen peroxide by degrading oxalic acid. Both of these molecules have been implied in the regulation of cell development, differentiation, signalling and defence systems of plants. The germin-like proteins found in dicotyledonous …

The Effect Of Sulphur Dioxide On Macrophage Phagocytic Activity In Diabetic Rats, Veci̇he Ni̇met İzgüt Uysal, Vural Küçükatay, Aysel Ağar, Pi̇raye Yargiçoğlu

Turkish Journal of Biology

Sulphur dioxide as a criterion of air pollution supresses macrophage functions. Some immunological changes in diabetes mellitus have been detected. In the present study, we examined the effect of sulphur dioxide on macrophage phagocytic activity in diabetic rats. The animals were separated into four groups as: 1) Control, 2) Sulphur dioxide, 3) Diabetes mellitus, 4) Diabetes mellitus+sulphur dioxide. The first and the third groups of animals were respirated with filtered atmosphere air in a special chamber one hour daily for eight weeks, while the other groups were exposed to 10 ppm sulphur dioxide in the same conditions and periods. The …

Physiological Changes In Grapevines Induced By Osmotic Stress Originated From Salt And Their Role In Salt Resistance, Nuray Si̇vri̇tepe

Turkish Journal of Biology

Rooted cuttings of Çavuş (salt-resistant), Müşküle (salt-sensitive) and Sultani Çekirdeksiz (moderately salt-sensitive) grapevine cultivars, grown in perlite medium, were subjected to salt stress by irrigating them with 1/2 Hoagland nutrient solution including different NaCl concentrations (0.00, 0.50 and 0.75%). It was determined that stomatal conductance and transpiration were strongly inhibited in Müşküle and Sultani Çekirdeksiz with salt treatments, while these physiological activities were maintained with a controlled decrease in cv. Çavuş. Relative water content of leaves decreased while loss of turgor increased in cv. Müşküle due to the salt treatments. Similar changes were also observed in cv. Sultani Çekirdeksiz although …

Transcriptional Regulation Of Chicken Apolipoprotein A-I Gene Expression, Wen Lin Kumfert

Theses and Dissertations in Biomedical Sciences

Deregulation of a set of critical cellular genes has long been speculated as a cause of the oncogenic transformation induced by v-Jun oncoprotein. In the past several years, extensive efforts have been made to identify such genes. Several target genes have been identified that are specifically associated with the v-Jun induced transformation phenotype in chicken embryo fibroblasts (CEF).

We have undertaken the objective to identify and characterize the genes that become deregulated in response to, or as a consequence of, Jun-induced transformation. By exploiting the difference in oncogenic potential between v-Jun and c-Jun in CEF, the chicken apolipoprotein A-I (apoA-I) …