Life Sciences Commons^™

Project Report No. 47, Loblolly Pine Plantations In East Texas Two Harvest Schedules, No Thinning, Final Harvest At 25 Years Thin At 15 Years Final Harvest At 25 Years Sawlog/Veneer Wood Flow Comparison, A Simulation, J. David Lenhart, Jennifer Allen

Informal Project Reports

DOES A THINNING AT IS YEARS WITH FINAL HARVEST AT 25 YEARS INCREASE SAWLOG/VENEER WOOD FLOW? DOES A THINNING AT IS YEARS WITH FINAL HARVEST AT 25 YEARS INCREASE NET PRESENT VALUE?

The Carboxyl Terminus Of The Bacteriophage T4 Dna Polymerase Is Required For Holoenzyme Complex Formation, Anthony J. Berdis, Patrice Soumillion, Stephen J. Benkovic

Chemistry Faculty Publications

To further elucidate the mechanism and dynamics of bacteriophage T4 holoenzyme formation, a mutant polymerase in which the last six carboxyl-terminal amino acids are deleted, was constructed, overexpressed, and purified to homogeneity. The mutant polymerase, designated ΔC6 exo−, is identical to wild-type exo− polymerase with respect to kcat, kpol, and dissociation constants for nucleotide and DNA substrate. However, unlike wild-type exo− polymerase, the ΔC6 exo− polymerase is unable to interact with the 45 protein to form the stable holoenzyme. A synthetic polypeptide corresponding to the carboxyl terminus of the wild-type exo− polymerase was tested as an in vitro inhibitor of …

Species Composition, Abundance, And Vertical Distribution Of The Stomiid (Pisces: Stomiiformes) Fish Assemblage Of The Gulf Of Mexico, Tracey Sutton, T. L. Hopkins

Marine & Environmental Sciences Faculty Articles

Species composition, abundance, and vertical distribution of the stomiid fish assemblage were investigated in the eastern Gulf of Mexico, a low-latitude, oligotrophic oceanic ecosystem. Seventy-two described species, representing 18 genera, and one undescribed species were identified from 1155 trawl samples. With an additional 10 species reported elsewhere, the stomiid species number now known equals 83, making the Stomiidae the most diverse fish family in the Gulf of Mexico. The assemblage was dominated by three species,Photostomias guernei, Chauliodus sloani and Stomias affinis. These species, as well as four other common species, exhibited an asynchronous diel vertical migration pattern (450–900 …

High-Resolution Time-Series Data For 1991/1992 Primary Production And Related Parameters At A Palmer Lter Coastal Site: Implications For Modeling Carbon Fixation In The Southern Ocean, Mark A. Moline, Barbara B. Prezelin

Biological Sciences

Our goal was to provide a high-resolution temporal data base for modeling primary production in shelf waters adjacent to Palmer Station, Antarctica. Here, the resulting 1991/1992 data base is used to: (1) determine in situ productivity over a range of seasonal to subseasonal time scales; (2) identify time scales of significant variability in marine productivity during the peak growing season; (3) identify environmental, experimental and analytical factors that can significantly impact the accuracy of daily, weekly and seasonal productivity estimates; and (4) integrate our findings with previous studies of Antarctic coastal primary pro- duction. Data were gathered every 2–3 days …

Kentucky Warbler (Vol. 72, No. 4), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Genetic And Social Control Of Male Maturation In Phallichthys Quadripunctatus (Pisces: Poeciliidae), Gita R. Kolluru, David N. Reznick

Biological Sciences

Age and size at maturity can have significant fitness consequences. Selection often favors early-maturing individuals because of their higher survival to maturity and greater relative contribution to population growth rate, but it may also favor delayed maturation if fitness increases with age or size at maturity. Males of several poeciliid fishes exhibit variation in age and size at maturity primarily controlled by a sex-linked gene called the P-locus. Wild-caught Phallichthys quadripunctatus males show a bimodal size distribution, which is often associated with a P-locus polymorphism in other poeciliids. We conducted two experiments to evaluate the inheritance of male age and …

Phylogenetic Analysis And Taxonomic Revision Of The Perdita Subgenera Macrotera, Macroteropsis, Macroterella And Cockerellula (Hymenoptera: Andrenidae), Bryan N. Danforth

Da

No abstract provided.

Nebline, November 1996

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Holiday food safety
Lancaster County Extension Educator recognized
Over the garden fence
Snow molds
Harvesting and storing winter squash
November garden hints
Winterizing roses
Winter care for strawberry plants
Harvesting popcorn
"Preparing Ag Professionals for Tomorrow's Challenges"
Emergency conservation program
1996 pesticide container recycling program nets over 17,000 containers
Beef Basics Home Study Courses
Precision Decisions '96
Report illegal dumping!
Household hazardous waste collection on November 2
The aluminum age
Wood for your fireplace
Which spider's the biter? You can't tell from looking at the bite!
Bt corn: environmentally friendly pest control
Cleaning closets? What to do with those unwanted …

Observation Of Spatial Variability Of Diurnal Thermocline And Rain-Formed Halocline In The Western Pacific Warm Pool, Alexander Soloviev, Roger Lukas

Marine & Environmental Sciences Faculty Articles

High-resolution measurements of temperature and salinity were made in the near-surface layer of the ocean during the Tropical Oceans-Global Atmosphere Coupled Ocean-Atmosphere Response Experiment, using probes mounted on the bow of the R/V Moana Wave. Because of surface waves and pitching of the vessel, the bow probes profiled the near-surface layer of the ocean within depths of 0–4.0 m. In the near-surface layer of the ocean in the western Pacific warm pool, strong variability of temperature and salinity produced by diurnal heating and/or rain was often observed. The contoured density field revealed cases of pronounced spatial variability. The shallow …

A Re-Evaluation Of Hotspot Settlement In Lekking Sage Grouse, Robert M. Gibson

School of Biological Sciences: Faculty Publications

Recent analyses of avian leks have come to conflicting conclusions concerning the role of male settlement on female traffic hotspots. This issue was re-examined in the sage grouse, Centrocercus urophasianus, using data on prenesting movements of radio-tagged females and the dispersion of lekking males collected during a 10-year field study. As expected with hotspot settlement, leks were preferentially located in areas through which females traveled between wintering and nesting ranges before mating. In addition, the distribution of males among leks was related proximately to variation in numbers of females visiting each lek during the mating period and ultimately to …

Transmittance And Reflectance Measurements Of Corn Leaves From Plants With Different Nitrogen And Water Supply, James S. Schepers, T. M. Blackmer, Wallace Wilhelm, M. Resende

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen is essential for crop production, but also contributes to eutrophication of surface water and degradation of drinking water quality. Modern corn production requires relatively large quantities of N, which are generally supplied by fertilizers. Over-application of N fertilizers and animal wastes frequently results in nitrate leaching. Synchronizing N availability with crop N need offers the potential to protect the environment without sacrificing production. Tools are needed to rapidly and easily monitor crop N status to make timely decisions regarding fertilizer application. Analytical and optical techniques were evaluated with greenhouse grown corn at silking to evaluate several methods to monitor …

Cellular Basis Of Decreased Immune Responses To Pneumococcal Vaccines In Aged Mice, Manju Garg, Wei Luo, Alan M. Kaplan, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previously, model systems were developed in our laboratory to study murine immune responses to the 23-valent pneumococcal polysaccharide vaccine Pnu-Imune, both in vivo and in vitro (M. Garg and B. Subbarao, Infect. Immun. 60:2329-2336, 1992; M. Garg, A. M. Kaplan, and S. Bondada, J. Immunol. 152: 1589-1596, 1994). Using these systems, we found that aged mice did not respond to the vaccine in vivo or in vitro. Cell separation studies showed that the unresponsiveness of the aged spleen cells to the vaccine was not due to an intrinsic B-cell defect or to T-cell-mediated immunosuppression but resulted from an accessory cell …

Effect Of Sediment Particle Size And Temperature On Fecal Bacteria Mortality Rates And The Fecal Coliform/Fecal Streptococci Ratio, J. M. Howell, Mark S. Coyne, P. L. Cornelius

Plant and Soil Sciences Faculty Publications

Extended survival of fecal bacteria in sediment can obscure the source and extent of fecal contamination in agricultural settings. The variability in fecal coliform/fecal streptococci (FC/FS) ratios with time and discrepancies between observable fecal sources and measured FC/FS ratios in shallow surface water from agricultural watersheds may be explained by examining FC and FS mortality rates in response to ambient temperature and sediment particle size. We measured FC and FS mortality rates at three different temperatures and in three feces-amended sediments with different particle size in a laboratory study. In controlled conditions, using physiological saline to reduce cell death by …

Western Australian Primary Industry And Fisheries Portfolios - Major Achievements 1993 - 1996, Department Of Primary Industry And Fisheries, Western Australia

Farm Systems and Economics published reports

AGRICULTURE and fishing were WA's first industries. Farm production in particular, was the State's economic mainstay for many years. It re1mains crucial lo our prosperity, and its future holds enormous potential.

As well as employment and income, agriculture and fisheries provide important recreation and landscape opportunities for our highly urbanised population, rural communities and international visitors. Recreational fishing- which is extremely popular - and the emerging farm tourism industry arc direct beneficiaries of strategics developed for sustainable primary industries.

Trade-Offs, Food Web Structure, And The Coexistence Of Habitat Specialists And Generalists, Mark A. Mcpeek

Dartmouth Scholarship

Species differ greatly in the breadth of their environmental distributions. Within the same collection of habitats, some species occur in many habitats, while others are only able to exist in one of a few. Trade-offs in the abilities of species to perform in various ecological interactions are important both to facilitating species coexistence within a habitat and to limiting the distributions of species among habitats. In this article I use a food web model to explore how in the same collection of habitats some species may be limited by trade-offs to occupying only one habitat, while other species may face …

Circadian Clock-Controlled Genes Isolated From Neurospora Crassa Are Late Night- To Early Morning-Specific, Deborah Bell-Pedersen, Mari L. Shinohara, Jennifer J. Loros, Jay C. Dunlap

Dartmouth Scholarship

An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control of genes involved in clock output pathways. In the fungus Neurospora crassa, one pathway known to be under control of the clock is asexual spore (conidia) development. To understand more fully the processes that are regulated by the N. crassa circadian clock, systematic screens were carried out for genes that oscillate at the transcriptional level. Time-of-day-specific cDNA libraries were generated and used in differential screens to identify six new clock-controlled genes (ccgs). Transcripts specific for each of the ccgs …

Mechanisms Of Cytokine-Mediated Localized Immunoprotection., Nora Sarvetnick

Journal Articles: Regenerative Medicine

No abstract provided.

Defining Arealistic Control For The Chloroform Fumigation-Incubation Method Using Microscopiccounting And 14c-Substrates, William R. Horwath, Eldor A. Paul, David Harris, Jeanette M. Norton, Leslie Jagger, Kenneth A. Horton

Plants, Soils, and Climate Faculty Publications

Chloroform fumigation-incubation (CFI) has made possible the extensive characterization of soil microbial biomass carbon (C) (MBC). Defining the non-microbial C mineralized in soils following fumigation remains the major limitation of CFI. The mineralization of non-microbial C during CFI was examined by adding ¹⁴C-maize to soil before incubation. The decomposition of the ¹⁴C-maize during a 10-d incubation after fumigation was 22.5% that in non-fumigated control soils. Re-inoculation of the fumigated soil raised ¹⁴C-maize decomposition to 77% that of the unfumigated control. A method was developed which varies the proportion of mineralized C from the unfumigated soil …

Parotid Gland Lipoma: A Rare Entity, S Muzaffar, N Kayani, S H. Hasan

Department of Pathology and Laboratory Medicine

No abstract provided.

A Highly Sensitive Plant Hybrid Protein Assay System Based On The Spm Promoter And Tnpa Protein For Detection And Analysis Of Transcription Activation Domains, Michael Schläppi, Ramesh Raina, Nina V. Fedoroff

Biological Sciences Faculty Research and Publications

TnpA is a multifunctional DNA binding protein encoded by the maize Suppressor-mutator (Spm) transposable element. TnpA is required for transposition and is a repressor of the unmethylated Spm promoter. While analyzing protein domains using a yeast GAL4-based hybrid system in transiently transformed tobacco cells, we found that TnpA represses the >10-fold transcriptional activation observed when the GAL4 DNA-binding domain is used alone. By contrast, compared to the backgroundless TnpA DNA-binding domain alone, 33- to 45-fold activation of the Spm promoter was observed when the VP16 activation domain was fused to it. TnpA-binding sites, but no TATA box, …

Human Herpesvirus Type 8 Dna Sequences In Cell-Free Plasma And Mononuclear Cells Of Kaposi's Sarcoma Patients, William J. Harrington, Omar Bagasra, Carlos E. Sosa, Lisa E. Bobroski, Marianna Baum, Xue Lan Wen, Lisa Cabral, Gerald E. Byrne, Roger J. Pomerantz, Charles Wood

Nebraska Center for Virology: Faculty Publications

Human herpesvirus (HHV) type 8 bas been detected in both classical and AIDS-related Kaposi's sarcoma, body-cavity lymphomas, and other types of tumors. HHV-8 has also been detected in DNA from peripheral blood mononuclear cells (PBMC) of some Kaposi's sarcoma patients and more readily in B cell fractions derived from panned cell subpopulations. Two patients were followed using several methods: in situ hybridization, solution-based polymerase chain reaction (PCR), and in situ PCR. HHV-8 was intermittently detected in plasma and detection correlated with detection in PBMC. In situ PCR demonstrated HHV-8 sequences in both peripheral blood B lymphocytes and, to a lesser …

The Use Of Bauxite Residue As A Soil Amendment In The Peel-Harvey Coastal Plain Catchment - Report On Results Of 1995 Environmental Monitoring Program, Mark Rivers

Natural resources published reports

This report was submitted in accordance with the recommendations of the Environmental Protection Authority (EPA) who have stated that a monitoring program shall be undertaken by Agriculture Western Australia within six months of the widespread use of bauxite residue as a soil amendment. Although this widespread use of bauxite residue has not yet formally commenced, pre-emptive monitoring has been undertaken in order to gain some understanding of the general environmental characteristics of the Peel-Harvey catchment and, in particular, the characteristics and quality of the water flowing through the catchment.

It represents the second annual report submitted to the EPA describing …

Western Australian Salinity Action Plan, Agriculture Western Australia, Department Of Conservation And Land Management, Department Of Environmental Protection, Water And Rivers Commission

Natural resources published reports

Salinity is one of the State's most critical environmental problems, threatening major economic and social consequences. It is preventable and, in the long term, reversible. Complete elimination is not realistic, but we can dramatically reduce its impact.

Salinity is caused by replacing deep-rooted native plants with shallow-rooted crops and pastures. More rainfall passed below the root zone and accumulates as groundwater so that watertables rise. The groundwater mobilises natural salts in the soil as it rises and carries them towards the surface, eventually degrading land and streams.

Using The Spot-Mapping Censusing Technique To Assess Changes In Breeding Bird Communities At Santa Ana National Wildlife Refuge, Alamo, Texas, 1940-1996, Alberto Cantu

Theses and Dissertations - UTB/UTPA

Results of breeding-bird censuses done by Davis in 1940 and by Gehlbach between 1973-1978 at Santa Ana National Wildlife, Alamo Texas, were compared to a 1995-1996 bird survey done in Gehlbach's study area. According to the Wilcoxon rank-sum test, bird densities in 1940 and 1995-1996 were similar, while 1973-1978 densities were lower. The 1995-1996 bird community was 76% similar to both the earlier studies. However, the communities studied in 1940 and 1973-1978 had only a 60% similarity. Negligible differences in bird densities and species composition were found between the 1995 and 1996 communities. Differences are attributed to changes in vegetation …

Atomic Force Microscopy Of Humic Acids, A. Ikai, R. Osterberg

Scanning Microscopy

Atomic force microscopic (AFM) images of humic acids show discrete, globular particles, where particles of the order of magnitude 100 to 300 nm dominate the image fields; the humic acids had been grown to a steady state at pH 5.0. The AFM data are consistent with our previously reported small-angle neutron scattering (SANS) study done under similar conditions. In further agreement, the cluster-cluster interactions shown in our previous SANS study may have their counterparts in closely interacting particles appearing as twin particles in the AFM images.

Energy Metabolism, Enzymatic Flux Capacities, And Metabolic Flux Rates In Flying Honeybees, R. K. Suarez, J. R.B. Lighton, B. Joos, S. (Stephen) P. Roberts, J. F. Harrison

Biological Sciences Faculty Research & Creative Works

Honeybees Rely Primarily on the Oxidation of Hexose Sugars to Provide the Energy Required for Flight. Measurement of VCO₂ (Equal to VO₂, Because VCO₂/VO₂ = 1.0 during Carbohydrate Oxidation) during Flight Allowed Estimation of Steady-State Flux Rates through Pathways of Flight Muscle Energy Metabolism. Comparison of V(_Max) Values for Flight Muscle Hexokinase, Phosphofructokinase, Citrate Synthase, and Cytochrome C Oxidase with Rates of Carbon and O₂ Flux during Flight Reveal that These Enzymes Operate Closer to V(Max) in the Flight Muscles of Flying Honeybees Than in Other Muscles Previously Studied. Possible Mechanistic …

Estimation Of Direct And Maternal Breed Effects For Prediction Of Expected Progeny Differences For Birth And Weaning Weights In Three Multibreed Populations, F. A. Rodriguez-Almeida, L. Dale Van Vleck, K. E. Gregory

Department of Animal Science: Faculty Publications

Direct and maternal breed effects on birth and 200-d weights were estimated for nine parental breeds (Hereford [H], Angus [A], Braunvieh [B], Limousin [L], Charolais [C], Simmental [S], Gelbvieh [G], Red Poll [R], and Pinzgauer [P]) that contributed to three composite populations (MARC I = ¼B, ¼C, ¼L, ⅛H, ⅛A; MARC II = ¼G, ¼S, ¼H, ¼A; and MARC III = ¼R, ¼P, ¼H, ¼A). Records from each population, the composite plus pure breeds and crosses used to create each composite, were analyzed separately. The animal model included fixed effects of contemporary group (birth year-sex-dam age), proportions of individual and …

Preparation Of Cultured Smooth Muscle Cells From Human Myometrium For X-Ray Microanalysis, Jarin Hongpaisan, Godfried M. Roomans

Scanning Microscopy

Methodological aspects of the use of X-ray microanalysis in physiological and pharmacological experiments on cultured myometrial cells were investigated. Cultured human myometrial cells were grown from biopsies after detaching the fibroblasts. Of the cultured cells, 95-98% showed desmin-like immunoreactivity. Transmission electron microscopy showed that subcultured cells were different from myometrial cells in situ. The effects of washing the cells to remove external salt-rich medium were investigated. All solutions removed the external medium, resulting in lower concentrations of Na and Cl. In the cells washed with 0.3 M mannitol, most of the elemental concentrations were significantly lower than in their …

The Vascular Flora Of The Lula Lake Land Trust, R. David Sapp

Honors Theses

The present study at the Lula Lake Land Trust centered on making a detailed and comprehensive list of the vascular flora contained within its boundaries. The collection of specimens began in May 1995 and extended through July 1996, for a total of fourteen months. The field work resulted in 412 specimens, comprising 79 families, 178 genera, and 252 species. The Lula Lake Land Trust is a non-profit, privately owned entity dedicated to the conservation and preservation of the Lula Lake area. It is in an alliance with The Nature Conservancy of Georgia, the Tennessee Aquarium, and the University of Tennessee …

Morphological And Chemical Properties Of Plastic Residues In Composts, Charles R. Krause, Leona E. Horst, Harry A. J. Hoitink

Scanning Microscopy

The relative quantity of plastic film residues and other man-made materials in composts prepared from municipal solid wastes (MSW), biosolids, yard wastes, and pine bark was evaluated utilizing light and scanning electron microscopy equipped with energy dispersive X-ray analyzer. MSW composts screened through a 4 mm screen contained significant but highly variable quantities of plastic film residues and other man-made materials that escaped detection with the unaided eye. The other composts were free of such particles. We conclude that the database for evaluation of man-made materials in MSW composts is inadequate and must be developed further.