Life Sciences Commons^™

In Vivo Detection Of Porcine Reproductive And Respiratory Syndrome Virus Rna By In Situ Hybridization At Different Times Post-Infection, Jung-Hyang Sur, Vicki Cooper, Judith A. Galeota, Richard A. Hesse, Alan R. Doster, Fernando A. Osorio

Nebraska Center for Virology: Faculty Publications

We studied the distribution of porcine reproductive and respiratory syndrome virus (PRRSV) RNA in tissues by in situ hybridization at different times postinfection (p.i.). The probe used for in situ hybridization was prepared by reverse transcription of PRRSV RNA, followed by PCR amplification of the cDNA. The sequence amplified corresponded to 433 bp from PRRSV open reading frame 7, which is contained in the nucleocapsid protein gene and which is highly conserved in both European and American strains (H. Mardassi, L. Wilson, S. Mounir, and S. Dea, J. Clin. Microbiol. 32:2197–2203, 1994). An immunohistochemical technique was used to detect PRRSV …

Taking Root, W. John Hayden

Biology Faculty Publications

The brief description of mangrove reproduction in John N. Cole's "Off Key, Out of Reach" [A Sense of Place, July-August] contains an error.

Challenges In The Quest For Keystones, M. E. Power, D. Tilman, J. A. Estes, B. A. Menge, W. J. Bond, L. Scott Mills, G. Daily, J. C. Castilla, J. Lubchenco, R. T. Paine

Wildlife Biology Faculty Publications

Identifying keystone species is difficult-but essential to understanding bow loss of species will affect ecosystems.

Characterization Of Experimentally Induced, Nonaflatoxigenic Variant Strains Of Aspergillus Parasiticus., Shubha Kale Ireland, J. W. Cary, D. Bhatnagar, J. W. Bennett

Faculty and Staff Publications

Six previously isolated, nonaflatoxigenic variants of Aspergillus parasiticus, designated sec mutants, were characterized morphologically by electron microscopy, biochemically by biotransformation studies with an aflatoxin precursor, and genetically by Northern (RNA) hybridization analysis of aflatoxin biosynthetic gene transcripts. Scanning electron micrographs clearly demonstrated that compared with the parental sec+ forms, the variant sec forms had an abundance of vegetative mycelia, orders of magnitude reduced number of conidiophores and conidia, and abnormal metulae. Conidiospores were detected in sec cultures only at higher magnifications (x500), in contrast to the sec+ (wild-type) strain, in which abundant conidiospores (masking the vegetative mycelia) were observed even …

Prevalence Of Antibodies To Feline Parvovirus, Calicivirus, Herpesvirus, Coronavirus, And Immunodeficiency Virus And Of Feline Leukemia Virus Antigen And The Interrelationship Of These Viral Infections In Free-Ranging Lions In East Africa, Regina Hofmann-Lehmann, Daniela Fehr, Markus Grob, Muhamed Elgizoli, Craig Packer, Janice S. Martenson, Stephen J. O'Brien, Hans Lutz

Biology Faculty Articles

While viral infections and their impact are well studied in domestic cats, only limited information is available on their occurrence in free-ranging lions. The goals of the present study were (i) to investigate the prevalence of antibodies to feline calicivirus (FCV), herpesvirus (FHV), coronavirus (FCoV), parvovirus (FPV), and immunodeficiency virus (FIV) and of feline leukemia virus (FeLV) antigen in 311 serum samples collected between 1984 and 1991 from lions inhabiting Tanzania’s national parks and (ii) to evaluate the possible biological importance and the interrelationship of these viral infections. Antibodies to FCV, never reported previously in free-ranging lions, were detected in …

Table Of Contents Volume Seven, Number Four, Fall 1996, Risk Editorial Board

RISK: Health, Safety & Environment (1990-2002)

Table of contents for the journal RISK: Health, Safety & Environment (ISSN: 1073-8673). Includes announcement for the First Biennial International Meeting of the Risk Assessment & Policy Association (RAPA) held in Alexandria, Virginia.

Review Of: Science Advice To The President (William T. Golden, Ed. Aaas Press 2d Ed. 1993)., Timothy Van Dyke

RISK: Health, Safety & Environment (1990-2002)

Review of: Science Advice to the President (William T. Golden, ed. AAAS Press 2d Ed. 1993). Acknowledgments, index of names, introductions, notes, tables. ISBN 0-87168-509-4 [340 pp. $29.95 paper. American Association for the Advancement of Science, 1333 H St. NW, Washington DC 20701.]

Pancreatic Expression Of Interleukin-4 Abrogates Insulitis And Autoimmune Diabetes In Nonobese Diabetic (Nod) Mice., Regula Mueller, Troy Krahl, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Diabetes in nonobese diabetic (NOD) mice is a T cell-dependent autoimmune disease. The destructive activities of autoreactive T cells have been shown to be tightly regulated by effector molecules. In particular, T helper (Th) 1 cytokines have been linked to diabetes pathogenesis, whereas Th2 cytokines and the cells that release them have been postulated to be protective from disease. To test this hypothesis, we generated transgenic NOD mice that express interleukin (IL) 4 in their pancreatic beta cells under the control of the human insulin promoter. We found that transgenic NOD-IL-4 mice, both females and males, were completely protected from …

Monoclonal Antibodies For Detection Of The H7 Antigen Of Escherichia Coli, Yongsheng He, James E. Keen, Ralph B. Westerman, E. Travis Littledike, Jimmy Kwang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two murine monoclonal antibodies (MAbs) (2B7 and 46E9-9) reactive with the H7 flagellar antigen of Escherichia coli were produced and characterized. A total of 217 E. coli strains (48 O157:H7, 4 O157:NM, 23 O157:non-H7, 22 H7:non-O157, and 120 non-O157:non-H7), 17 Salmonella serovars, and 29 other gramnegative bacteria were used to evaluate the reactivities of the two MAbs by indirect enzyme-linked immunosorbent assay (ELISA). Both MAbs reacted strongly with all E. coli strains possessing the H7 antigen and with H23- and H24-positive E. coli strains. Indirect ELISA MAb specificity was confirmed by inhibition ELISA and by Western blotting (immunoblotting), using partially …

Time Of Lambing: Extension Manual, Keith Croker, Ian Mcfarland

Agriculture reports

No abstract provided.

Activation Of The Human Thymidine Kinase (Tk) Promoter By Simian Virus 40 Large T Antigen Requires Both The T Antigen Prb Family-Binding Domain And Tk Promoter Sequences Resembling E2f-Binding Sites., Michelle M. Anderson, Jun Chen, Charles N. Cole, Susan E. Conrad

Dartmouth Scholarship

Infection of quiescent cells with the DNA tumor virus simian virus 40 induces expression of the cellular thymidine kinase (TK) gene a minimum of 10- to 20-fold, and this induction depends upon the viral protein large T antigen (T-Ag). To define both human TK promoter elements and T-Ag functional domains required for transcriptional induction, we have established a system in which stable Rat-1 transfectants harboring TK promoter-luciferase hybrid genes are infected with recombinant adenoviruses expressing either wild-type or mutant forms of T-Ag and luciferase expression is measured as an indicator of promoter activity. The results show that (i) a 135-bp …

A Late Baird's Sandpiper In Keith County, Stephen J. Dinsmore

Nebraska Bird Review

On 23 December 1994, Gordon Brown and I were walking the North Platte River below Keystone Dam as part of the Lake McConaughy Christmas Bird Count (CBC). At approximately 8:30 a.m. MST, about 0.5 mi below the dam, we observed a group of 8 Killdeer and a smaller shorebird, which we immediately recognized as a "peep." We studied the bird for about 30 minutes at distances as close as 40 feet and then returned in the afternoon to photograph it. The size and black legs immediately eliminated Least Sandpiper, and the dark rump eliminated White-rumped Sandpiper. The remaining possibilities were …

Masthead And Table Of Contents Nebraska Bird Review 64(3) September 1996

Nebraska Bird Review

Table of Contents

A Review of the Status of Limnodromus griseus, the Short-billed Dowitcher, in Nebraska ……………..74

A Late Baird’s Sandpiper in Keith County ……………..79

Another Common Crane in Nebraska with a Summary of North American Records…………….. 80

Nebraska Ornithologists’ Union Annual Meeting 18 May 1996…………….. 82

NOU and NAMC Spring Bird Counts May 1996…………….. 83

Table of Bird Species Observed during NOU Annual Meeting 17-19 May 1996, and NAMC 11 May 1996 in Two Counties…………….. 84

Summer Field Report June-July 1996 ……………..90

Observers for Summer Field Report ……………..103

Nou And Namc Spring Bird Count, May 1996

Nebraska Bird Review

Bird species seen in a Nebraska five-county area during the Annual Meeting of the Nebraska ornithologists' Union 17-19 May, are presented in Table 1 on pages 84-90. The total count was 161 species.

The table also includes data for the North American Migration count on 11 May in two Nebraska counties, Boone (compiler: Wayne Mollhoff, Ashland), and Sarpy (compiler: Betty Grenon, Bellevue). Observations in Boone Co. were made between 9:00 a.m. and 6:00 p.m. with two observers for 6 hours and one observer for 3 hours. They covered 3 miles on foot for 6 hours and 40 miles by car …

Summer Field Report, June-July 1996, W. Ross Silcock, Joel G. Jorgensen

Nebraska Bird Review

Starting with this report, Joel Jorgensen will write the reports for sub-passerines. Hopefully this will help with timeliness, especially in getting reports to the Regional Editor for Audubon Field Notes. It is a long process writing up especially the Spring and Fall reports, given the excellent number received.

Highlights in this report are rare loons at Lake McConaughy, three reports of Clark's Grebe, King Rail in Seward Co, Sandhill Cranes in Clay Co, Mountain Plovers in Kimball Co, easterly reports of Black-necked Stilt, first breeding record for Wilson's Phalarope in the Rainwater Basin, Brown Creeper in Sarpy Co, Blue-gray Gnatcatcher …

Nebraska Bird Review (September 1996) 64(3), Whole Issue

Nebraska Bird Review

A Review of the Status of Limnodromus griseus, the Short-billed Dowitcher, in Nebraska ... 74

A Late Baird’s Sandpiper in Keith County ... 79

Another Common Crane in Nebraska with a Summary of North American Records … 80

Nebraska Ornithologists’ Union Annual Meeting 18 May 1996 ... 82

NOU and NAMC Spring Bird Counts May 1996 ... 83

Table of Bird Species Observed during NOU Annual Meeting 17–19 May 1996, and NAMC 11 May 1996 in Two Counties ... 84

Summer Field Report June–July 1996 ... 90

Observers for Summer Field Report … 103

A Recent Record Of Mountain Lion In Nebraska, Hugh H. Genoways, Patricia W. Freeman

University of Nebraska State Museum: Mammalogy Papers

On the morning of November 10, 1991, a deer hunter in the Pine Ridge area of extreme northwestern Nebraska shot a young female mountain lion (Gertzen, 1991, Omaha World-Herald, November 15, Sunrise ed. p. 17sf; Henion, 1991, Omaha World-Herald, November 15, Metro ed. p. 17). Although mountain lions (Felis concolor) were part of the original mammalian fauna of Nebraska (Jones, 1949, J. Mammal. 30: 313; 1962, Bull Univ. Nebraska State Mus. 4: 87-100; 1964, Univ. Kansas Publ., Mus. Nat. Hist. 16: 1-356), the species has been considered to be extirpated from the state. The most recent record that …

Imaging God: God's Earth, Human Worlds, And The Challenge Of Agriculture, Loren Wilkinson

Pro Rege

This article is based on a paper read at the Dordt College Bioethics Conference held February 19-20, 1996.

Managing Pastures For Long-Term Prosperity, Hugh Pringle, Jenny Riches

Natural resources published reports

A Workshop held by the Sandstone Land Conservation District Committee

Lake Mason Station

April 1994

A workshop hosted by Sandstone Land Conservation District Committee (LCDC) was held at Lake Mason station on 28 April 1994. This workshop was based on two previous workshops hosted by the Kalgoorlie and north-eastern Goldfields LCDCs in May 1991 (Pringle et al. 1992). The success of the earlier workshops prompted the rangeland survey team in association with the Sandstone LCDC and the Kalgoorlie District Office of Agriculture Western Australia to initiate the project.

The aims were to provide a summary of local wisdom regarding pasture …

Cropwatch No. 96-20, Aug. 30, 1996, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Sugar beet diseases ........... 136

Evapotranspiration data ... 136

Grasshopper control ......... 137

Hessian fly/pest controls .. 138

Wheat seed treatments ..... 139

Weather outlook ................ 140

Late season weeds ............ 140

Predicting last irrigation.. 141

Kansas pest update ........... 142

Regulation Of Ribulose Bisphosphate Carboxylase Gene Expression In Natural Phytoplankton Communities .1. Diel Rhythms, S. L. Pichard, L. Campbell, J. B. Kang, F. Robert Tabita, John H Paul

Marine Science Faculty Publications

Marine phytoplankton fix carbon dioxide primarily through the action of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the first enzyme in the Calvin Cycle. Although the regulation of this enzyme has been studied in algal cultures and higher plants, little is known regarding RubisCO regulation in natural phytoplankton populations. To determine if natural communities of phytoplankton utilize transcriptional regulation to control RubisCO expression, we investigated the diel relationship between ¹⁴C carbon fixation and RubisCO large subunit (rbcL) transcript levels, rbcL DNA, chlorophyll a, autofluorescent cell counts and bacterial direct counts for natural communities of the southeastern Gulf of Mexico. Studies …

Hydrogen Atom Extraction And Redeposition On Hydrogen-Terminated Silicon Surface With Scanning Tunneling Microscope At Room Temperature, D. H. Huang, Y. Yamamoto

Scanning Microscopy

We have previously demonstrated that hydrogen atoms on the hydrogen-terminated Si(100)-2xl:H surface can be extracted by applying either positive or negative voltage pulses between an STM tip and the sample surface with a certain tunneling current. This phenomenon suggests that electron excitation and field evaporation both play a role in atom transfer. Under our experimental conditions, hydrogen atoms are extracted in pairs from either a single dimer or from two adjacent dimers in neighboring rows. Hydrogen atoms can also be redeposited onto the Si(100)-2xl:H surface from a tungsten tip, which has picked up hydrogen from the sample surface through the …

Verbena Hastata L., Bob Edgin

Specimens by Name

No abstract provided.

Applications Of Laser Microprobe Mass Spectrometry In Biology And Medicine, Annette Bakker, Luc Van Vaeck, Wim Jacob

Scanning Microscopy

Laser microprobe mass spectrometry (LMMS) provides spot analysis with a lateral resolution of 1-5 μm. Focused laser ionisation, combined with mass spectrometry, yields information on elements, molecular identification of inorganic substances, and structural characterisation of organic molecules of typically less than 2-3 kDa. Quantification in complex heterogeneous systems, such as biological materials, is not possible at the present state of the art. The strength of the method lies in the qualitative information on the molecular composition of the analyte, not just element detection, with a lateral resolution at the light microscopy level. The applications of this technique in the field …

Influence Of Bleeding On Post-Mortem Tenderization Of Fish Muscle During Chilled Storage, M. Ando, A. Nishiyabu, T. Nakagawa, Y. Makinodan

Scanning Microscopy

The influence of bleeding on post-mortem tenderization of fish muscle during storage was studied. Six fish species were used for the present study. Among them, delay of post-mortem tenderization of muscle by bleeding was shown in the pelagic fishes: yellowtail, horse mackerel, and striped jack. On the other hand, bleeding had no influence on the muscle firmness of the bottom fishes: red sea bream, flatfish, and rudder-fish. According to transmission electron microscopy, degradation of pericellular collagen fibrils was delayed in bled yellowtail and horse mackerel. In the case of striped jack, though, collagen fibril degradation could not be observed; slower …

The Role Of Calcium In The Regulation Of Apoptosis, David J. Mcconkey

Scanning Microscopy

The recognition that apoptosis is regulated by an evolutionarily conserved set of polypeptides from the nematode Caenorhabditis elegans to humans suggests that a conserved set of biochemical mechanism(s) may also be involved in the response. Early evidence suggested that the endogenous endonuclease implicated in apoptosis in most model systems is Ca2+ -dependent, and subsequent work from a number of independent laboratories suggests that alterations in cytosolic Ca2+ homeostasis are one of the conserved biochemical pathways regulating the response. Molecular targets for Ca2+ are now being identified and include signal transduction intermediates, endonuclease(s) and proteases, and the enzymes involved in the …

12th Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

Contact Force Dependence On Relative Humidity: Investigations Using Atomic Force Microscopy, E. Finot, E. Lesniewska, J. -C. Mutin, S. I. Hosain, J. -P. Goudonnet

Scanning Microscopy

This paper deals with the ability of scanning force microscopy to determine contact forces of various materials. Indeed, with high spring constants at low relative humidity, the nature of the material can be determined by measurement of the contact force as the tip approaches. Cantilevers with a high spring constant are used to achieve solid-solid contact for the tip-sample system. The capillary force estimation provides information on the development of the height of the water meniscus formed between the tip and different surfaces depending on the relative humidity. Finally, we focus our attention on measurements of moduli of elasticity which …

Covalent Labeling Of Proteins With Fluorescent Compounds For Imaging Applications, Darl R. Swartz

Scanning Microscopy

The labeling of proteins with fluorescent compounds for microscopy has allowed a greater understanding of biological processes. The preparation of fluorescent proteins is the first step in development of their use in microscopy. Methods are described to label and characterize a protein as an example of the general approach for other proteins. Skeletal muscle alpha-actinin was labeled with either fluorescein-5-maleimide or 5-iodoaceamidofluorescein and the reaction characterized. The maleimide reaction was much more rapid and efficient than the iodoacetamide reaction giving a coupling efficiency of 65% under the given ration conditions. The fluorescein-5-maleimide alpha-actinin was functionally characterized and there was essentially …

Cropwatch No. 96-19, Aug. 16, 1996, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

ECB update.................132

Evapotranspiration data.................132

Millet for feed.................133

Winter wheat meetings.................133

Herbicide Guide.................133

Restricted entry intervals.................134

Nitrogen use.................134