Life Sciences Commons^™

An In-Depth Study Of Fruit Preservation: Effect Of Calcium Chloride And Methyl Jasmonate Applications On Quality And Biochemical Properties Of Cherry Fruits During Cold Storage, Muhammet Ali̇ Gündeşli̇

Turkish Journal of Agriculture and Forestry

Sweet cherry (Prunus avium L.) is a highly perishable fruit that undergoes rapid quality deterioration during postharvest storage. This study evaluated the effects of postharvest applications of Methyl Jasmonate (MeJA, 1 mM) and Calcium Chloride (CaCl₂, 2%), applied individually and in combination, on maintaining the quality and biochemical properties of cherries during 21 days of cold storage at 0 ± 1 °C. Key parameters including weight loss, decay rate, soluble solids content (SSC), pH, titratable acidity, respiration rate, phenolic profile, and organic acid composition were monitored. The combined MeJA + CaCl₂ treatment was consistently more effective than individual applications in …

Estimation Of Genetic Parameters In Lemon-Scented Basil (Ocimum Basilicum L.) Lines, Erdi̇nç Günay, İsa Telci̇

Turkish Journal of Agriculture and Forestry

Selective breeding of basil (Ocimum basilicum L.) is primarily aimed at improving plant architecture and essential oil quality, which are critical factors in increasing yield. In lemon-scented basil, the citral component responsible for the intense lemony aroma has significant commercial value in the cosmetics and perfumery industries. This study aimed to evaluate the genetic variability, heritability, and genetic advance of nine agronomic and quality related traits in lemon basil lines, and to explore the interrelationships among these traits. The phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV) for all traits, suggesting the influence of …

Landscape Character Analysis And Assesment At Local Scale, Eli̇f Önal, Serkan Özer

Turkish Journal of Agriculture and Forestry

​Landscape areas are one of the most important elements of human life all over the world. Varied landscape areas are a valuable natural resource and have high cultural value. The first step to ensure the effective landscape use is to identify these areas, analyze them in detail, and determine their characteristics. The northeastern Anatolia region in Türkiye has a rich diversity of landscapes because of varied geographic, climatic, and topographic features. The aim of this study was to identify the landscape character types in the Tortum district, located in the Tortum River basin in northeastern Türkiye, and to assess the …

Sewage Sludge Legislation In Central Europe: Contamination Concerns And Cross-Border Comparisons, Valerie Vranová, Aleš Kučera, Vlastimil Skoták, Erika Fialová, Rahul Datta

Turkish Journal of Agriculture and Forestry

Sewage sludge management represents a major challenge in Europe, carrying significant environmental and legal implications that require strong regulatory enforcement. Here, we explore the legislative measures, technological developments, and environmental impacts of sewage sludge management in Central Europe (Czechia, Slovakia, Germany, Austria, and Poland). Sludge management is regulated at the EU level through a series of directives (e.g., Council Directive 86/278/EEC, which forms the basis of national regulatory frameworks). However, national adaptations vary considerably: Croatia and Slovenia have invested in sludge phosphorus-recovery systems, while Poland and Slovakia have focused on the agricultural use of sludge as a fertilizer. Several countries, …

Crispr-Cas9: An Emerging And Precise Genome Editing System For Crops To Combat Food Insecurity, Muhammad Faran Tahi̇r, Muhammad Hamzah Saleem, Sidra Aslam, Emi̇ne Orhan, Hayat Topçu, Tauqeer Ahmed Qadri, Khalid Ali Khan, Muhammad Anas

Turkish Journal of Agriculture and Forestry

Genome editing has emerged as a transformative tool to improve crop productivity, resilience, and nutritional quality, helping address global food insecurity. Among gene-editing platforms, clustered regularly interspaced short palindromic repeats (CRISPR)/ Cas systems (notably CRISPR-Cas9) offer simple, efficient, and scalable methods for targeted modifications, including knockouts, base edits, and precise “search-and-replace” prime edits. In plants, recent advances such as improved base editors, optimized prime-editing platforms, and DNA-free delivery methods have expanded the scope of edits achievable while reducing off-target effects and regulatory concerns. This review summarizes CRISPR principles, highlights technical breakthroughs and crop applications that mitigate biotic and abiotic stresses, …

In Vitro Propagation Of A New Clonal Apple Rootstock Candidate Hatay, Zeynep Nas, Ahmet Eşi̇tken, Lütfi̇ Pirlak

Turkish Journal of Agriculture and Forestry

​Some clonal apple rootstocks are traditionally propagated using the layering technique. However, traditional propagation methods are seasonal and require a lot of space and labor. In addition, the multiplication rate is low with these methods, resulting in low-profit sapling production for growers. In vitro propagation techniques provide an efficient and effective method of obtaining species-appropriate, quality saplings in less time and space. The study aims to determine the in vitro propagation protocol of the Hatay genotype and to establish an appropriate method. In the study, the highest number of shoots was observed in media containing BA and mT at concentrations …

Cultivar, Canopy Architecture, And Harvest Stage Shape The Chemical Profile Of Plum (Prunus Domestica) Fruit, Jelisaveta Seka Cvijanović, Milica Sredojević, Dragana Dabić Zagorac, Maja Natić, Geza Bujdoso, Miljan Cvetković

Turkish Journal of Agriculture and Forestry

Plum (Prunus domestica L.) is a nutritionally valuable fruit with significant commercial importance, yet consumer acceptance is often constrained by inconsistent fruit quality. This study investigated the effects of cultivar, training system, and harvest stage on the chemical composition of plum fruit, focusing on polyphenols, organic acids, sugars, and sugar alcohols. Three cultivars (Čačanska lepotica, Stanley, and Empress) were evaluated under different canopy architectures, including plum spindle as a control and novel 2-dimensional systems (Bi axe and upright fruiting offshoot), across multiple ripening stages. The results showed that genotype and canopy structure significantly influenced the accumulation of primary and secondary …

Enhancing Salinity Tolerance In Phyla Nodiflora L. With Potassium Humate: Crosstalk Between Osmolytes, Antioxidants, And Mineral Uptake, Fatemeh Sadeghi, Somayeh Esmaeili, Mehrangiz Chehrazi

Turkish Journal of Agriculture and Forestry

Salinity stress impairs plant growth, nutrient homeostasis, and metabolism, particularly in arid ecosystems. Phyla nodiflora, a ground cover used for erosion control, shows preliminary salinity tolerance, but the mechanistic basis of its long-term adaptation and the potential for enhancement via potassium humate (KH) were unknown. Plant growth, key biochemical attributes, and nutrient uptake were assessed to elucidate how KH modulates physiological performance under increasing salinity. In a 129-day greenhouse experiment, plants were exposed to five salinity levels (0 to 16 dS m⁻¹ NaCl) and three KH concentrations (0, 500, and 1000 mg L⁻¹). Severe salinity (16 dS m⁻¹) without KH …

Distribution And Seasonal Prevalence Of Nosema Maddoxi (Microsporidia: Nosematidae) In The Brown Marmorated Stink Bug, Halyomorpha Halys (Hemiptera: Pentatomidae) Populations In Türkiye, Ömer Ertürk, Samet Eker, Mustafa Yaman, Gönül Algi

Turkish Journal of Agriculture and Forestry

The brown marmorated stink bug, Halyomorpha halys feeds on a wide range of crops and causes major economic losses. With its high flight capacity, H. halys is an invasive species that damages many crops worldwide. By elucidating the activity, distribution, and prevalence of the microsporidian pathogen Nosema maddoxi, the damage and economic losses caused by H. halys can be reduced. This study aimed to assess the distribution and seasonal prevalence of N. maddoxi in H. halys populations distributed throughout hazelnut fields the Eastern Black Sea Region of Türkiye. H. halys adults were collected and examined in detail under the microscope …

Genetic Algorithm For Agricultural Logistics Optimization: Solving The Capacitated Vehicle Routing Problem In Rural Distribution, Min Song, Kan Lu

Turkish Journal of Agriculture and Forestry

With the rapid advancement of agricultural modernization and the increasing demand for agricultural products, inefficient logistics distribution has become a major bottleneck in rural supply chains. This study addresses the capacitated vehicle routing problem (CVRP) in agricultural logistics. A genetic algorithm (GA)-based optimization model was proposed to enhance distribution efficiency. The model integrates critical agricultural characteristics, including multidistribution center networks, seasonal delivery schedules, and regional road infrastructure constraints, to minimize both transportation distance and operational costs. Experimental results show that the GA outperforms traditional metaheuristic methods (e.g., particle swarm optimization and simulated annealing), achieving a >5 km reduction in total …

Genetic Diversity And Population Structure Of Carob Germplasm From Lebanon, Morocco, And Spain Reveal Potential For Innovative Breeding Programs, Mohamad Ali El Chami, Guillermo Palacios-Rodriguez, Rafael Maria Navarro-Cerrillo, Lamis Chalak, Maria Dolores-Rey

Turkish Journal of Agriculture and Forestry

The carob tree (Ceratonia siliqua L.) is a drought-tolerant species native to the Mediterranean Basin. It has been cultivated for centuries for its highly nutritious, edible pods. Genetic diversity is one of the key requirements for the effective management and utilization of plant genetic resources. In this study, we evaluated the genetic diversity and population structure of 169 seminatural carob individuals from Lebanon, Spain, and Morocco using nine expressed sequence tag-simple sequence repeat (EST-SSR) markers. A total of eight polymorphic EST-SSR loci produced 43 alleles, with Cesi_187 and Cesi_1187 identified as the most informative markers. Results of AMOVA and SAMOVA …

Molecular Characterization Of Pasteurella Multocida Capsular Type A Strains Isolated From Different Regions Of Anatolia, Türkiye​, Adam Bashir Tawor, Osman Ergani̇ş, Asli Balevi̇, Suliman Mohamed Yousof Sadam

Turkish Journal of Veterinary & Animal Sciences

Pasteurella multocida serotype A is an important pathogen that causes respiratory illness in both farm animals and poultry, leading to severe economic losses worldwide. This study aimed to isolate P. multocida capsular type A (capA) as well as investigate the virulence-associated genes (VAGs) within the isolates. Samples (n = 250) were collected in the Anatolia region from cattle (n = 130), sheep (n = 70), goats (n = 45), and pet birds (n = 5) with clinical respiratory signs. Polymerase chain reaction (PCR) assays were conducted using kmt1 and capA primers for P. multocida species identification and capsular serogroup A …

Trace Elements In Complete Feeds Used In Intensive Rearing Of Cattle, Poultry, And Pigs: Occurrence And Predicted Transferred Concentrations In Animal-Derived Food, Elizabeta Dimitrieska Stojkovikj, Vangelica Enimiteva, Aleksandra Angjeleska, Risto Uzunov

Turkish Journal of Veterinary & Animal Sciences

This study investigated the concentrations of essential (Zn, Cu, Mn, Co, and Mo) and toxic (Pb, Cd, As, and Hg) trace elements in complete feeds used for the intensive rearing of cattle, poultry, and pigs in North Macedonia and predicted their transfer into animal-derived foods. A total of 107 feed samples were collected from 40 farms between May 2023 and April 2024 and analyzed using inductively coupled plasma mass spectrometry. The results revealed that while most essential elements were within the regulatory limits, Co exceeded the maximum level in two cattle feed samples. Zn, Cu, and Mn levels were generally …

Effect Of Rangeland Quality On Rumen Development Of Angora (Mohair) Goat Kids And Bucks, Betül Zehra Sariçi̇çek

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effect of rangeland quality on the rumen development of Angora goat kids and bucks. Twenty male singleton Angora goat kids with similar birth dates and birth weights were selected from each farm in an area with three different rangeland quality levels (good, medium, or poor) in Ankara Province. The kids were weaned at 60 days of age and then grazed completely on rangeland until slaughter. Rumen volumetric and papillary development of animals slaughtered at 4 and 7 months of age were determined. Empty rumen length, width, and weight were affected by rangeland quality …

Arterial Architecture Of The Goat Spleen (Capra Hircus) Revealed By Corrosion Casting And Scanning Electron Microscopy, Baran Erdem, Sevi̇nç Ateş, Meryem Karan

Turkish Journal of Veterinary & Animal Sciences

The spleen is an essential organ involved in hematopoietic and immune functions. A comprehensive understanding of its anatomy, including vascular topography and segmentation, is essential for the accurate diagnosis and treatment of splenic diseases, and the minimization of complications during surgical procedures. Detailed knowledge of the vascular architecture also supports the accurate prediction and management of hemorrhage severity in cases of splenic rupture. The spleen’s microvascular system has a unique and complex organization that is distinct from other organs, and its elucidation is critical for understanding splenic function. In the present study, the spleens of 20 hair goats were examined. …

Hard Palate Morphology In The Anatolian Water Buffalo (Bubalus Bubalis), Mehmet Can, Şükrü Hakan Atalgin, Mehmet Faruk Aydin, Soner Bekmezci̇

Turkish Journal of Veterinary & Animal Sciences

This study aimed to examine the morphological and histological features of the hard palate in the Anatolian water buffalo through light and scanning electron microscopy, complemented by gross and morphometric evaluations. Eight hard palates were used in the study. A total of eight Anatolian water buffalo heads from healthy adult female water buffaloes were collected immediately after slaughter from various slaughterhouses in Balıkesir, Türkiye. After analyzing and identifying the obtained hard palate samples, measurements were taken and documented through photography. The papilla incisiva, located in the middle of the caudal concave edge on the ventral surface of the pulvinus dentalis, …

Full Issue, Volume 14, Number 1., Donna J. Peterson Dr., Scott Cummings, Elizabeth Gregory North

Journal of Human Sciences and Extension

No abstract provided.

New Locality Records And Future Habitat Suitability Of Sassanidotus Gracilis (Birula, 1900) (Scorpiones: Buthidae) In The Iran–Afghanistan–Pakistan Arid Belt, Ersen A. Yağmur, Kerim Çiçek, Homa Barati, Mohammad Moradi

Euscorpius

This study reports new locality records from Iran and a climate-based habitat suitability assessment for Sassanidotus gracilis (Birula, 1900) (Scorpiones: Buthidae) in the Iran–Afghanistan–Pakistan arid belt. We document new specimens from Sistan and Baluchestan Province, Iran, with an updated distribution map and illustrations, and we model the species' potential distribution across south-eastern Iran and adjacent parts of Afghanistan and Pakistan.

Habitat suitability was associated mainly with precipitation seasonality, precipitation of the wettest month, precipitation of the driest month, and slope. In an arid setting these rainfall variables are unlikely to indicate a need for moist habitat; they more plausibly track …

Effects Of Human-Animal Interaction On Positive Youth Development: A Replication Study, Allison Pachunka, Jay Jeffries, Lisa Karr, Lena Luck, Bryan A. Reiling, Douglas H. Schultz, Jeffrey R. Stevens

People and Animals: The International Journal of Research and Practice

Interacting with animals can improve health and well-being across the lifespan. Some programs such as 4-H and FFA center around youth interacting with animals, potentially enhancing youth development. Mueller (2014) investigated whether these interactions and attitudes about animals influenced measures of positive youth development. The aim of our studies was to replicate Mueller’s methods and extend her work by evaluating whether membership in animal-focused youth programs moderate associations between interactions, attitudes, and positive youth development. We did not find strong relationships among human–animal inter-action and positive youth development, which failed to replicate Mueller’s findings. However, we did find relationships between …

Evaluating The Potential Of Cajanus Cajan (L.) Millsp. Root To Improve Atopic Dermatitis With Different Anti-Inflammatory Models And Active Compounds, Shu-Er Yang, Xiang-Ming Cai, Yu-Wen Chu, Jen-Chieh Tsai, Mey-Fann Lee, Tuzz-Ying Song

Journal of Food and Drug Analysis

Atopic dermatitis (AD) is a chronic inflammatory disease characterized by recurrent bacterial infections that cause pruritus, erythema, xerosis and thickening. Cajanus cajan root extract prepared with 95% ethanol (EECR95) has been shown to possess antioxidant, anti-inflammatory, and antibacterial properties in previous studies. This study aimed to evaluate EECR95’s ability to improve AD by inhibiting methicillin-resistant Staphylococcus aureus (MRSA) growth and reducing inflammation. This study found EECR95 effectively inhibits MRSA growth (IC₅₀ = 103.74 μg/mL) and reduces pro-inflammatory cytokine production in an MRSA-keratinocyte-macrophage model. LC/MS/MS analysis revealed the presence of active compounds- flavonoids, isoflavones, and flavonoid glycosides, highlighting EECR95's potential …

Clara S. Ludlow: Mississippi A & M Graduate And American Military Entomology Pioneer, Bradley Brazzeal

Midsouth Entomologist

The systematic study of the mosquitoes of the world did not begin until the early years of the 20^th century. Among the early entomologists to specialize in the field was Clara S. Ludlow, who graduated from Mississippi Agricultural and Mechanical College. Her research began in the Philippines in 1901, while American troops were still fighting in the Philippine-American War, and for over 20 years she researched mosquitoes in connection with the U.S. Army. This article explores Ludlow’s scientific career and the legacy of her research.

First Insular Record And Dna Barcoding Of Euscorpius Vailatii Tropea & Fet, 2015 From Trizonia Island (Fokida, Greece), Konstantinos Kalaentzis, Francesco Frigioni, Alexios Panagiotopoulos, Elias Tzoras, Styliani Minoudi, Alexandros Triantafyllidis

Euscorpius

Euscorpius vailatii Tropea & Fet, 2015 (Scorpiones: Euscorpiidae), originally described from Aitoloakarnania and Fokida (Greece), is herein reported from Trizonia Island in the Gulf of Corinth, representing the first insular record for the species. The examined specimens are consistent with the diagnostic morphology of E. vailatii. The first molecular data for the species are provided, based on both mainland and insular populations. Comparative analyses further highlight the distinctiveness of E. vailatii from geographically close congeners, while phylogenetic results indicate a sister-taxon relationship with E. erymanthius, supporting a Quaternary biogeographic history shaped by the evolution of the Gulf of …

Transcriptomic Profiling Of The Donkey Endometrium Reveals Dynamic Molecular Transitions Across The Estrous Cycle, Limeng Shi, Shuaishuai Wu, Mingyue Zhao, Wenhao Zhou, Abd Ullah, Muhammmad F. Akhtar, Changfa Wang, Muhammad Z. Khan, Ying Han

The Thai Journal of Veterinary Medicine

The endometrium undergoes extensive cyclical remodeling essential for reproductive success; however, the molecular mechanisms governing these processes in donkeys (Equus asinus) remain poorly characterized. To address this gap, we performed high-throughput RNA sequencing on endometrial tissues collected from Dezhou donkeys (n = 16) at four post-ovulation stages: Day 0 (ovulation), Day 3 (early luteal), Day 7 (mid-luteal), and Day 18 (late luteal/pre-luteolysis). Differential gene expression analysis, Gene Ontology enrichment, and KEGG pathway mapping were conducted to characterize temporal transcriptomic dynamics. We identified 2,474, 6,548, and 1,288 differentially expressed genes in Day 0 versus Day 3, Day 0 versus …

Adaptive Phishing Url Detection Using Hybrid Fuzzy C-Means Clustering And Xgboost, Muntadher Mohammed Kareem, Rawaa I. Farhan

Karbala International Journal of Modern Science

Phishing attacks continue to evolve in sophistication, rendering static detection methods increasingly ineffective. Existing URL-based approaches suffer from limited adaptability to emerging phishing patterns, mislabeled training data, and insufficient validation protocols. This paper proposes a hybrid phishing URL detection system that integrates Fuzzy C-Means (FCM) clustering with XGBoost classification, enhanced by a novel Micro Adaptive Feature Extractor (MAFE). The system employs a multi-stage pipeline: feature engineering generating 36 statistical and interaction features, MAFE producing 15 adaptive features through class-aware dynamic weighting, micro-pattern detection, and entropy analysis, and FCM with K=2 clusters providing soft membership features to XGBoost. A two-pass confidence-based …

Occupational Impacts Of Compound Resistance Training In Community-Dwelling Older Adults, Alexis Skalberg, Jennifer Muriithi

Journal of Emerging Scholars in Occupational Therapy

Background

Existing literature highlights the importance of resistance training as an intervention to counteract age-related declines such as loss of muscle mass, decreased strength, and diminished cognitive function. Despite evidence supporting resistance training in older adults, its integration into occupational therapy practices remains underexplored.

Research Design

Six adults over age 65 living in Northwest Arkansas participated in a quasi-experimental pretest-posttest study investigating the impact of compound resistance training on participation in preferred and necessary daily activities, as well as mental health. Subjects completed an initial assessment consisting of a semi-structured interview, the Patient Health Questionnaire, the Perceived Stress Scale, and …

Enzymatic Synthesis Of Salicylate In Yersina Pestis Proceeds Via Intramolecular Hydroxyl Group Transfer., Clarke Miller

Georgia Journal of Science

The salicylate synthase (YbtS) of Yersinia pestis catalyzes the initial step in the biosynthesis of yersiniabactin, an iron-scavenging siderophore and critical virulence factor. While YbtS is proposed to share a  reaction mechanism with other anthranilate synthase homologs, the exact catalytic pathway has remained unresolved.  In this work, stable isotope (¹⁸O) labeling and mass spectrometry are utilized to clarify the catalytic mechanism of YbtS.  The results of the following set of experiments demonstrate that the salicylate hydroxyl group does not originate from solvent water, directly challenging previously proposed models. Instead, the lack of solvent-labeled oxygen incorporation and the absence …

Snails In The Diet Of The Cutlip Minnow, Exoglossum Maxillingua, Tess A. Taloumis, Steven L. Powers

Southeastern Fishes Council Proceedings

The Cutlip Minnow, Exoglossum maxillingua, is an important stream fish native to the eastern United States. It has an unusual lower jaw morphology. Early hypotheses suggested the jaw morphology is an adaptation for feeding on snails. Despite the publication of numerous diet studies on this species, there is no documentation of snails as prey items. During recent snorkel observations in the Roanoke River, E. maxillingua was spotted carrying snails within their mouths, prompting further investigation. An in-depth temporal examination of the diet from 145 specimens of E. maxillingua across multiple sites was conducted to further investigate the diet of …

Comparative Analysis Of Artificial Intelligence (Ai) Implications For Active Learning And Assessment At Various Curricular Levels Of Stem And Health Sciences In Developed Versus Underprivileged Countries, Vinay Munlapudi, Santanu De

FDLA Journal

Today’s all-pervasive emergence of artificial intelligence (AI) necessitates a comprehensive evaluation of its role in education, especially for occupational disciplines in health sciences and STEM. AI has reshaped student learning and instructors’ navigation of the evolving, post coronavirus infectious disease (COVID-19) global academic landscape. This study aimed at encapsulating evidence-based AI implications for student-centered, active learning and assessment at various curricular levels of science, technology, engineering, mathematics (STEM) and health sciences education in developed versus underprivileged or less developed countries. An extensive, scoping review of 65 recent, scholarly publications was conducted over six months through diverse search engines (PubMed, Google …

In Vitro Digestion Modifies Lepidium Meyenii Extract, Altering Its Major Glucosinolates, Lepidilines, Β-Carbolines, And Gut Bacterial Growth, Dominik Tarabasz, Ewa Baranowska-Wójcik, Paweł Szczeblewski, Tomasz Laskowski, Klaudia Gustaw, Marzena Furtak-Niczyporuk, Dominik Szwajgier, Rita Cristina Orihuela-Campos, Henry O. Meissner, Julia Borzyszkowska-Bukowska, Wirginia Kukula-Koch

Journal of Food and Drug Analysis

The tubers of Lepidium meyenii (commonly known as maca) have been used in the Andean cuisine for ages. The study aims to determine the behavior of the extract from the red phenotype of maca in the in vitro gastrointestinal tract model and also to define the overall impact of the extract on the microbiota. Glucosinolates, lepidilines, and a derivative of β-carboline alkaloid, i.e. 1-methyltetrahydro-β-5,6-hydride-carboline-3-carboxylic acid, as representatives of one of the main groups of metabolites were selected for the quantitative analysis after in vitro digestion at every stage of the digestion model. The results showed that lepidilines …

Descriptive Anatomy Of The Female Reproductive Organs In Thamin Eld’S Deer (Rucervus Eldii Thamin) And Fea’S Muntjac (Muntiacus Feae) With Utilizing Of The Glycerin Impregnation Technique For Specimen Preservation, Paisan Tienthai, Pawana Chuesiri, Itti Boonorrana, Piyaporn Kongmakee, Ampika Thongphakdee

The Thai Journal of Veterinary Medicine

The reproductive anatomy of rare cervid species remains insufficiently documented, limiting comparative studies and preservation efforts. This study describes the anatomical details in the female reproductive organs of Thamin Eld’s deer (Rucervus eldii thamin) and Fea’s muntjac (Muntiacus feae), while the application of a modified glycerin impregnation technique was achieved for specimen preservation. Dissections of female reproductive tracts revealed morphological variations that correlate with the known female reproductive characteristics of domestic and closely related species. In both species, the ovaries were characterized as small, ovoid structures, exhibiting prominent follicles or corpora lutea consistent with the stage …