Life Sciences Commons^™

Evaluation Of 9,10 Anthraquinone Application To Pre-Seed Set Sunflowers For Repelling Blackbirds, Megan D. Niner, George M. Linz, Mark E. Clark

United States Department of Agriculture Wildlife Services: Staff Publications

Most nonlethal methods available for reducing blackbird (Icteridae) damage to sunflowers rely on fright responses (e.g., propane cannons, distress calls, pyrotechnics, raptor silhouettes) that birds quickly learn to ignore. Chemicals that cause taste or feeding aversions have potential to overcome the spatial and behavioral limitations of frightening methods. Anthraquinone (AQ) is an effective feeding repellent as a seed treatment to deter birds from eating freshly planted grains. In the United States, foliar application of AQ is not permitted on food crops except on small experimental plots. In August 2013, we applied 37.4 L/ha of an aqueous mix consisting of 15.1 …

Multi-Trophic Level Feeding Interactions Among Two Native And Two Non-Native Species: Implications For The Endangered Reticulated Flatwoods Salamander (Ambystoma Bishopi), Kelly C. Jones, Brandon K. Rincon, Thomas A. Gorman, Carola A. Haas, Richard M. Engeman

United States Department of Agriculture Wildlife Services: Staff Publications

Eglin Air Force Base (Eglin) in Florida is one of few remaining public lands where the Reticulated Flatwoods Salamander (Ambystoma bishopi) occurs, and it represents the only remaining location within the entire range of this endangered species to have two populations that both occur in wetland complexes with >2 occupied wetlands (Gorman et al., 2009). The decline of the species has been rapid and few remaining breeding locations for this species remain. It was listed as federally endangered in 2009 (USFWS, 2009). Flatwoods salamanders depend on complex herbaceous vegetation for all aspects of their life history and while …

Sunflower Bird Pests, George M. Linz, James J. Hanzel

United States Department of Agriculture Wildlife Services: Staff Publications

Sunflower (Helianthus annuus L.) is annually planted on approximately 26 million hectares in Australia, China, Europe, India, North America, Paldstan, Russia, South America, and Ukraine (National Sunflower Association, 2014). Flocks of granivorous birds, ranging in size from a few to millions, can be found in every sunflower growing region and have been documented to cause serious economic losses (Linz et al., 2011) (Figure 7.1). Avian species that damage sunflower generally belong to the parakeet (Psittacldae), dove (Columbidae), cockatoo (Cacatuidae), sparrow (Passeridae), crow (Corvidae), and blackbird (Icterinae) families (De Grazio, 1989; Linz and Hanzel, 1997; Linz et al., 2011; Rodriquez …

Serologic Survey Of Snowshoe Hares (Lepus Americanus) In The Greater Yellowstone Area For Brucellosis, Tularemia, And Snowshoe Hare Virus, Jack Rhyan, Dan Tyers, Jeremy Zimmer, Kristen Lewandowski, Steve Hennager, John Young, Ryan Pappert, Amanda Panella, Olga Kosoy

United States Department of Agriculture Wildlife Services: Staff Publications

We examined sera from snowshoe hares (Lepus americanus) livetrapped in the northern Greater Yellowstone Area (GYA), US, for antibodies to Brucella abortus, Francisella tularensis, and snowshoe hare virus (SSHV). Zero of 90, 0 of 67, and 40 of 100 samples were antibody positive for B. abortus, F. tularensis, and SSHV, respectively. Hares were trapped from 2009 to 2012, and of the six animals that were captured twice with at least 1 yr between captures, four developed antibody to SSHV, indicating active exposure to the agent, These findings suggest snowshoe hares in the GYA do not play …

Food Habits Of Adult Male White-Tailed Deer Determined By Camera Collars, Michael J. Lavelle, Chad R. Blass, Justin W. Fischer, Scott E. Hygnstrom, David G. Hewitt, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Many factors influence what and why animals select the foods they eat. Several methods have been used to estimate food habits of herbivores, but they all have limitations such as defining available foods and misrepresenting particular forages. We evaluated plant consumption by adult male white-tailed deer (Odocoileus virginianus) with camera collars in a semi-enclosed population in southern Texas, USA, during late autumn 2010. We collected 1,241 videos taken at all times of the day and night from 15 camera collars and confirmed consumption of 40 plant species with an 84% probability of identification. Diets of individual deer varied …

Boa Constrictor, Are R. Berentsen, Juan G. Garcia-Cancel, Edna M. Diaz-Negron, Oscar A. Diaz-Marrero, Alberto R. Puente-Rolón, Robert N. Reed, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

No abstract provided.

Evaluating The Reliability Of Field Identification And Morphometric Classifications For Carnivore Scats Confirmed With Genetic Analysis, Robert C. Lonsinger, Eric M. Gese, Lisette P. Waits

United States Department of Agriculture Wildlife Services: Staff Publications

Scat surveys are commonly used to monitor carnivore populations. Scats of sympatric carnivores can be difficult to differentiate and field-based identification can be misleading. We evaluated the success of field-based species identification for scats of 2 sympatric carnivores—coyotes (Canis latrans) and kit foxes (Vulpes macrotis). We conducted scat surveys in the Great Basin desert of Utah, USA, during the winter and summer of 2013, and we detected 1,680 carnivore scats. We classified scats based on field identification, recorded morphometricmeasurements, and collected fecalDNA samples for molecular species identification. We subsequently evaluated the classification success of field identification …

Contact Heterogeneities In Feral Swine: Implications For Disease Management And Future Research, Kim M. Pepin, Amy J. Davis, James C. Beasley, Raoul Boughton, Tyler A. Campbell, Susan M. Cooper, Wesson D. Gaston, Steve Hartley, John C. Kilgo, Samantha M. Wisely, A. Christy Wyckoff, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Contact rates vary widely among individuals in socially structured wildlife populations. Understanding the interplay of factors responsible for this variation is essential for planning effective disease management. Feral swine (Sus scrofa) are a socially structured species which pose an increasing threat to livestock and human health, and little is known about contact structure. We analyzed 11 GPS data sets from across the United States to understand the interplay of ecological and demographic factors on variation in co-location rates, a proxy for contact rates. Between-sounder contact rates strongly depended on the distance among home ranges (less contact among sounders …

To Cross Or Not To Cross: Modeling Wildlife Road Crossings As A Binary Response Variable With Contextual Predictors, Shane R. Siers, Robert N. Reed, Julie A. Savidge

United States Department of Agriculture Wildlife Services: Staff Publications

Roads are significant barriers to landscape-scale movements of individuals or populations of many wildlife taxa. The decision by an animal near a road to either cross or not cross may be influenced by characteristics of the road, environmental conditions, traits of the individual animal, and other aspects of the context within which the decision is made. We considered such factors in a mixed-effects logistic regression model describing the nightly road crossing probabilities of invasive nocturnal Brown Treesnakes (Boiga irregularis) through short-term radiotracking of 691 snakes within close proximity to 50 road segments across the island of Guam. All …

Is There A Single Best Estimator? Selection Of Home Range Estimators Using Area-Under-The-Curve, W. David Walter, Dave P. Onorato, Justin W. Fischer

United States Department of Agriculture Wildlife Services: Staff Publications

Background: Global positioning system (GPS) technology for monitoring home range and movements of wildlife has resulted in prohibitively large sample sizes of locations for traditional estimators of home range. We used areaunder- the-curve to explore the fit of 8 estimators of home range to data collected with both GPS and concurrent very high frequency (VHF) technology on a terrestrial mammal, the Florida panther Puma concolor coryi, to evaluate recently developed and traditional estimators.

Results: Area-under-the-curve was the highest for Florida panthers equipped with Global Positioning System (GPS) technology compared to VHF technology. For our study animal, estimators of home …

Exposure Of Feral Swine (Sus Scrofa) In The United States To Selected Pathogens, John A. Baroch, Carl A. Gagnon, Sonia Lacouture, Marcelo Gottschalk

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine (Sus scrofa) are widely distributed in the United States. In 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the US to evaluate exposure to important swine endemic pathogens. Antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) were found in 2.5% and 25.3% of tested sera, respectively. Positive serological reactions against Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae have been detected in 19.7% and 69.7% of animals. More than 15% of animals presented antibodies against these 2 pathogens simultaneously. Most animals were also …

Evaluating Fladry Designs To Improve Utility As A Nonlethal Management Tool To Reduce Livestock Depredation, Julie K. Young, Elizabeth Miller, Anna Essex

United States Department of Agriculture Wildlife Services: Staff Publications

Nonlethal deterrents against carnivores are important components to protecting livestock and conserving carnivore populations. However, the performance of the visual deterrent called fladry, a historical tool used to defend livestock from carnivores, is often hindered by design flaws that eventually reduce its effectiveness. Our purpose was to identify a fladry design that reduces coiling (i.e., wrapping of individual flags tight to the rope from which they hang) and maintains free movement of the deterrent in the wind. We created 6 new designs, replicated designs using 2 materials (nylon and marine vinyl), and compared them with the design most commonly used …

Evaluating Behavioral Syndromes In Coyotes (Canis Latrans), Julie K. Young, Marion Mahe, Stewart W. Breck

United States Department of Agriculture Wildlife Services: Staff Publications

As more research focuses on behavioral syndromes and their role in ecological and evolutionary processes, it is imperative that methods to test behavior are valid. The objectives of this study were to (1) assess behavior in captive coyotes (Canis latrans) using three methods [agitation scores, novel object test, and flightinitiation distance (FID)] and (2) to determine whether the three tests were correlated within individuals to describe behavioral syndromes. Female coyotes had higher agitation scores during handling (2.6 ± 0.5) than males (1.5 ± 0.3; t = 1.90, p = 0.06): scores ranged from 0 to 8 on a …

Animal Reactions To Oncoming Vehicles: A Conceptual Review, Steven L. Lima, Bradley F. Blackwell, Travis L. Devault, Esteban Fernández-Juricic

United States Department of Agriculture Wildlife Services: Staff Publications

Animal–vehicle collisions (AVCs) are a substantial problem in a human-dominated world, but little is known about what goes wrong, from the animal’s perspective, when a collision occurs with an automobile, boat, or aircraft. Our goal is to provide insight into reactions of animals to oncoming vehicles when collisions might be imminent. Avoiding a collision requires successful vehicle detection, threat assessment, and evasive behaviour; failures can occur at any of these stages. Vehicle detection seems fairly straightforward in many cases, but depends critically on the sensory capabilities of a given species. Sensory mechanisms for detection of collisions (looming detectors) may be …

Widespread Detection Of Antibodies To Leptospira In Feral Swine In The United States, K. K. Pedersen, K. L. Pabilonia, T. D. Anderson, S. N. Bevins, C. R. Hicks, J. M. Kloft, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

As feral swine continue to expand their geographical range and distribution across the United States, their involvement in crop damage, livestock predation, and pathogen transmission is likely to increase. Despite the relatively recent discovery of feral swine involvement in the aetiology of a variety of pathogens, their propensity to transmit and carry a wide variety of pathogens is disconcerting. We examined sera from 2055 feral swine for antibody presence to six serovars of Leptospira that can also infect humans, livestock or domestic animals. About 13% of all samples tested positive for at least one serovar, suggesting that Leptospira infection is …

Anticoagulant Rodenticide Exposure And Toxicosis In Coyotes (Canis Latrans) In The Denver Metropolitan Area, Sharon A. Poessel, Stewart W. Breck, Karen A. Fox, Eric M. Gese

United States Department of Agriculture Wildlife Services: Staff Publications

Anticoagulant rodenticides are widely used in urban areas to control rodent pests and are responsible for secondary poisoning in many nontarget wildlife species. We tested the livers of five coyotes (Canis latrans) in the Denver Metropolitan Area, Colorado, US, for anticoagulant rodenticides. All five livers were positive for brodifacoum, with values ranging from 95 ppb to 320 ppb, and one liver was positive for bromadiolone, with a value of 885 ppb. Both of these rodenticides are second-generation anticoagulants, which are more potent and more likely to cause secondary poisoning than first-generation anticoagulants due to their accumulation and persistence …

The Effect Of Social Context And Social Scale On The Perception Of Relationships In Monk Parakeets, Elizabeth A. Hobson, Darlene J. John, Tiffany L. Mcintosh, Michael L. Avery, Timonthy F. Wright

United States Department of Agriculture Wildlife Services: Staff Publications

Social relationships formed within a network of interacting group members can have a profound impact on an individual’s behavior and fitness. However, we have little understanding of how individuals perceive their relationships and how this perception relates to our external measures of interactions. We investigated the perception of affiliative and agonistic relationships at both the dyadic and emergent social levels in two captive groups of monk parakeets (Myiopsitta monachus, n = 21 and 19) using social network analysis and playback experiments. At the dyadic social scale, individuals directed less aggression towards their strong affiliative partners and more aggression …

Antibody Response Of Cattle To Vaccination With Commercialmodified Live Rabies Vaccines In Guatemala, Amy T. Gilbert, Lauren Greenberg, David Moran, Danilo Alvarez, Marlon Alvarado, Daniel L. Garcia, Leonard Peruski

United States Department of Agriculture Wildlife Services: Staff Publications

Vampire bat rabies is a public and animal health concern throughout Latin America. Aspart of an ecological study of vampire bat depredation on cattle in southern Guatemala,we conducted a vaccine seroconversion study among three dairy farms. The main objec-tives of this cross sectional and cohort study were to understand factors associated withbat bites among cattle, to determine whether unvaccinated cattle had evidence of rabiesvirus exposure and evaluate whether exposure was related to bat bite prevalence, and toassess whether cattle demonstrate adequate seroconversion to two commercial vaccinesused in Guatemala. In 2012, baseline blood samples were collected immediately prior tointramuscular inoculation of …

A Behaviorally-Explicit Approach For Delivering Vaccine Baits To Mesopredators To Control Epizootics In Fragmented Landscapes, James C. Beasley, Todd C. Atwood, Michael E. Byrne, Kurt C. Vercauteren, Shylo R. Johnson, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

Despite the widespread use of aerial baiting to manage epizootics among free-ranging populations, particularly in rabies management, bait acceptance and seroconversion rates often are lower than required to eliminate spread of disease. Our objectives in this study, therefore, were to evaluate the performance of stratified bait distribution models derived from resource selection functions (RSF) on uptake of placebo rabies baits by raccoons (Procyon lotor) and Virginia opossums (Didelphis virginiana), as well as the probability of bait uptake as a function of proximity to bait distribution areas in fragmented agricultural ecosystems. Among 478 raccoons and 108 opossums …

Is Anthropogenic Cougar Mortality Compensated By Changes In Natural Mortality In Utah? Insight From Long-Term Studies, Michael L. Wolfe, David N. Koons, David C. Stoner, Patricia Terletzky, Eric M. Gese, David M. Choate, Lise M. Aubry

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding the interplay between exploitation and natural mortality is essential to guiding sustainable conservation of wildlife. Exploitation of carnivores by humans has long been thought to result in compensatory reductions of natural mortality among survivors. If rates of human exploitation exceed natural mortality, however, such actions will ‘add’ to overall mortality and could imperil the sustainability of such actions. We applied competing risk analyses to >/= 16 years of data for heavily harvested and semi-protected cougar populations in Utah to test the additive and compensatory mortality hypotheses, while accounting for parameter uncertainty. We additionally tested for presence of the two …

An Individual-Based Model For Feral Hogs In Great Smoky Mountains National Park, Rene A. Salinas, William H. Stiver, Joseph L. Corn, Suzanne Lenhart, Charles Collins, Marguerite Madden, Kurt C. Vercauteren, Brandon S. Schmit, Ellen Kasari, Agricola Odoi, Graham Hickling, Hamish Mccallum

United States Department of Agriculture Wildlife Services: Staff Publications

The expansion of feral hog (Sus scrofa) populations in the United States has resulted in increased efforts to develop and implement control strategies designed to minimize the impacts done by this invasive species. We describe an individual-based model for feral hogs in Great Smoky Mountains National Park (GSMNP). The objectives of the model are to provide an understanding of the population dynamics of this feral hog population and to determine the efficacy of the annual harvest as a population control method. Results suggest that the dynamics of the population are driven by fall hard mast production and the …

Evaluating Landowner-Based Beaver Relocation As A Tool To Restore Salmon Habitat, V. M. Petro, J. D. Taylor Ii, D. M. Sanchez

United States Department of Agriculture Wildlife Services: Staff Publications

Relocating American beavers (Castor canadensis) from unwanted sites to desirable sites (i.e., where damage exceeds stakeholder capacity) has been posited as a method to enhance in-stream habitat for salmonids in the Pacific Northwest region of the US; however, no studies have evaluated this method. From September–December 2011, we trapped and relocated 38 nuisance beavers using guidelines available to Oregon landowners. Release sites were selected from models that identified high values of beaverdamhabitat suitability and where dams would increase intrinsic potential of coho salmon (Oncorhynchus kisutch). Mean distance moved from release sites within 16 weeks post-release was 3.3 …

Assessment Of Population Structure Of Coyotes In East-Central Alabama Using Microsatellite Dna, Dalinda L. Damm, James B. Armstrong, Wendy M. Arjo, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Canis latrans (Coyotes) are a management concern in the southeastern US because of their potential impacts on agriculture, other wildlife species, and human health and safety. This region is part of a recent range expansion by Coyotes, and information about their population structure in the southeastern US is lacking. In this study, we used microsatellite DNA to assess genetic diversity and population structure among Coyotes in east-central Alabama. We detected high genetic diversity (H_E = 0.78) and no population structure across the total sampling area. Additionally, we investigated population structure between urban and rural groups. We detected low but …

Assessing Chemical Control Of Earthworms At Airports, Thomas W. Seamans, Bradley F. Blackwell, Glen E. Bernhardt, Daniel A. Potter

United States Department of Agriculture Wildlife Services: Staff Publications

Earthworms originating from Europe (e.g., Lumbricus spp., Aporrectodea spp.), which are common in the United States and southern Canada can create hazardous conditions at airports by attracting birds that pose a threat to aircraft. These nonnative earthworms are also considered pests on golf courses and sports fields, as well as having detrimental effects on temperate boreal forests. No toxicants or repellents are currently registered for earthworm control in the United States. Our purpose was to identify products that could be used to repel or suppress nonnative earthworms on airports or other managed sites where they pose a hazard or nuisance. …

Effects Of Mountain Beaver Management And Thinning On 15-Year-Old Douglas Fir Growth And Survival, Dan L. Campbell, Richard M. Engeman, James P. Farley

United States Department of Agriculture Wildlife Services: Staff Publications

We examined 4-year growth of 15-year-old damaged and undamaged Douglas fir (Pseudotsuga menzesii) after integrating temporary population reductions of mountain beaver (Aplodontia rufa) with thinning in a pre-commercial hand-planted plantation in western Washington. Five treatment combinations were considered: (1) trapping mountain beavers in an unthinned area, (2) trapping before thinning to 65 trees/ha (160 trees/ac), (3) no trapping and thinning to 65 trees/ha, (4) no trapping and thinning to 146 trees/ha (360 trees/ac), and (5) no trapping and no thinning. Removal of ≥90 % of mountain beavers temporarily reduced mountain beaver activity whether the stand was unthinned or thinned. Diameter …

Avian Bornaviruses In North American Gulls, Jianhua Guo, Ian Tizard, John A. Baroch, H L. Shivaprasad, Susan L. Payne

United States Department of Agriculture Wildlife Services: Staff Publications

Avian bornaviruses, recently described members of the family Bornaviridae, have been isolated from captive parrots and passerines as well as wild waterfowl in which they may cause lethal neurologic disease. We report detection of avian bornavirus RNA in the brains of apparently healthy gulls. We tested 439 gull brain samples from 18 states, primarily in the northeastern US, using a reversetranscriptase PCR assay with primers designed to detect a conserved region of the bornavirus M gene. Nine birds yielded a PCR product of appropriate size. Sequencing of PCR products indicated that the virus was closely related to aquatic bird bornavirus …

Review Of The 2012 Epizootic Hemorrhagic Disease Outbreak In Domestic Ruminants In The United States, G Stevens, B Mccluskey, A. King, E O'Hearn, G Mayr

United States Department of Agriculture Wildlife Services: Staff Publications

An unusually large number of cases of Epizootic hemorrhagic disease (EHD) were observed in United States cattle and white-tailed deer in the summer and fall of 2012. USDA APHIS Veterinary Services area offices were asked to report on foreign animal disease investigations and state diagnostic laboratory submissions which resulted in a diagnosis of EHD based on positive PCR results. EHD was reported in the following species: cattle (129 herds), captive white-tailed deer (65 herds), bison (8 herds), yak (6 herds), elk (1 herd), and sheep (1 flock). A majority of the cases in cattle and bison were found in Nebraska, …

The Cost Of Canine Rabies On Four Continents, A. M. Anderson, S. A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

We estimated the economic impacts of canine rabies in Latin America, Africa and Asia. Direct and indirect costs of rabies post-exposure prophylaxis, dog vaccination and control, rabies diagnostic testing and cattle mortality-related costs were accounted for. The number of human deaths was updated from previous estimates based on population growth, and the costs associated with the risk of human mortality were incorporated. We accounted for uncertainty associated with the parameter estimates using a Monte Carlo simulation and estimated that the global burden of canine rabies is approximately $124 billion annually. This result illustrates the potential benefits that could be realized …

Modeling The Cost Of Bird Strikes To Us Civil Aircraft, Aaron M. Anderson, David S. Carpenter, Michael J. Begier, Bradley F. Blackwell, Travis L. Devault, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

The objective of our analysis is to develop a model of damage costs that arise from collisions between aircraft and birds, based on data drawn from the Federal Aviation Administration National Wildlife Strike Database (NWSD). We develop a two-part model, composed of two separate statistical models, that accounts for the effects of aircraft mass category, engine type, component of the aircraft struck, and the size and number of birds struck. Our results indicate the size of bird, number of birds, and engine ingestions are the largest determinants of strike-related costs. More generally, our result is a model that provides a …

Serological Investigation Of Heartland Virus (Bunyaviridae: Phlebovirus) Exposure In Wild And Domestic Animals Adjacent To Human Case Sites In Missouri 2012–2013, Angela M. Bosco-Lauth, Nicholas A. Panella, J. Jeffrey Root, Tom Gidlewski, R. Ryan Lash, Jessica R. Harmon, Kristen L. Burkhalter, Marvin S. Godsey, Harry M. Savage, William L. Nicholson, Nicholas Komar, Aaron C. Brault

United States Department of Agriculture Wildlife Services: Staff Publications

Heartland virus (HRTV; Bunyaviridae: Phlebovirus) has recently emerged as a causative agent of human disease characterized by thrombocytopenia and leukopenia in the United States. The lone star tick (Amblyomma americanum L.) has been implicated as a vector. To identify candidate vertebrate amplification hosts associated with enzootic maintenance of the virus, sera and ticks were sampled from 160 mammals (8 species) and 139 birds (26 species) captured near 2 human case residences in Andrew and Nodaway Counties in northwest Missouri. HRTV-specific neutralizing antibodies were identified in northern raccoons (42.6%), horses (17.4%), white-tailed deer (14.3%), dogs (7.7%), and Virginia opossums …