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Some Hematological Parameters And Enzyme Histochemistry Of Peripheral Bloodleukocytes In Kivircik Sheep, Mehmet Faruk Aydin, İhsan Kisadere Jan 2022

Some Hematological Parameters And Enzyme Histochemistry Of Peripheral Bloodleukocytes In Kivircik Sheep, Mehmet Faruk Aydin, İhsan Kisadere

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine some hematological parameters (RBC, WBC, HCT, HGB, MCH, MCHC, MCV, and PLT), the presence and percentages of ANAE and AcP-ase positivities in leukocytes and the differential leukocyte count in Kivircik sheep. Blood samples were taken from the jugular vein of 40 (rams = 20; ewes= 20) healthy, 1-year-old sheep. MCH and MCHC values were found statistically higher in rams than ewes (p < 0.05). Mean percentages of ANAE-positive PBL were defined as 74.16 ± 11.94% in ewes and 70.33 ± 8.39% in rams. It was not determined any significant changes according to sex. On the other hand, the percentages of ACP were determined as 67.12 ± 6.54% in ewes and 66.71 ± 6.58% in rams. It was not found any significant differences according to sex. For ANAE enzyme activity, lymphocytes, monocytes, neutrophils, and eosinophilic granulocytes showed a positive reaction. For ACP enzyme activity, although lymphocytes presented positivity, monocytes, neutrophils, eosinophils were negative. The ratio of lymphocyte, monocyte, neutrophile, eosinophile, and basophile of Kivircik ewes and rams were found as 64.35 ± 5.74%, 60.64 ± 8.85%; 3.41 ± 1.27%, 3.82 ± 1.42%; 27.58 ± 5.46%, 30.94 ± 8.71%; 2.41 ± 0.93%, 2.88 ± 1.45%; 1.76 ± 0.75%, 1.29 ± 0.58%, respectively. The difference between mean values was not found significant according to sex (p > 0.05). In conclusion, our study showed that enzyme histochemical methods can be used for identifying peripheral blood cells and can provide facilitates for early diagnosis of immune system-related disorders …


A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska Jan 2022

A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to evaluate the luminol-dependent spontaneous and stimulated chemiluminescence (CL) of whole blood and milk in cows with subclinical and clinical mastitis. The research was carried out on 108 Holstein-Friesian dairy cows aged 3-5 years. Based on the assessment of somatic cell count (SCC) in quarter milk, the animals were divided into 3 groups: I - clinically healthy cows (n = 35), II - animals with subclinical mastitis (n = 30) and III - cows with clinical mastitis (n = 43). The material for the research was whole blood and quarter milk. The samples were …


The Impact Of Fusarium Mycotoxins On Macro And Micro Parameters Of Dairy Cattle, Rimvydas Falkauskas, Bronius Bakutis, Jurgita Jovaisiene, Gintare Vaiciuliene, Gediminas Gerulis, Rasa Zelvyte, Elena Bartkiene, Ignas Silinskas, Jolita Klementaviciute, Violeta Baliukoniene Jan 2022

The Impact Of Fusarium Mycotoxins On Macro And Micro Parameters Of Dairy Cattle, Rimvydas Falkauskas, Bronius Bakutis, Jurgita Jovaisiene, Gintare Vaiciuliene, Gediminas Gerulis, Rasa Zelvyte, Elena Bartkiene, Ignas Silinskas, Jolita Klementaviciute, Violeta Baliukoniene

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to evaluate the impact on macro and micro parameters of dairy cattle when cows consumed total mixed ration (TMR) contaminated with (zearalenone) ZEA and deoxynivalenol (DON) for 60 days. Macro indicators included milk composition and production and micro parameters included blood components, rumen volatile fatty acids (VFAs), and ZEA and DON concentrations in rumen fluid and milk. Twenty dairy cows were fed TMR with naturally contaminated mycotoxins (ZEA 300 - 500 μg/kg; DON 102 - 200 μg/kg). The trial lasted for 60 days. Samples of blood, rumen fluid, and milk were taken three …


Effects Of Rumen-Protected Methionine And Lysine On Milk Yield And Milk Composition Inholstein Dairy Cows Consuming A Corn Grain And Canola Meal-Based Diet, Emrah Gülgün, Eki̇n Sucu Jan 2022

Effects Of Rumen-Protected Methionine And Lysine On Milk Yield And Milk Composition Inholstein Dairy Cows Consuming A Corn Grain And Canola Meal-Based Diet, Emrah Gülgün, Eki̇n Sucu

Turkish Journal of Veterinary & Animal Sciences

Methionine (Met) and lysine (Lys) are thought to be the two most important amino acids for lactation performance. Met and Lys dietary supplementation can thus be an effective approach to improve amino acid balance for lactation performance, particularly in early lactation. The purpose of this study was to determine how rumen-protected (RP) Met + Lys affect milk production, milk composition, and feed efficiency in primiparous Holstein dairy cows fed a corn grain and canola meal-based diet for 60 days (8.5 weeks) following calving. Two hundred primiparous Holstein dairy cows were randomly assigned to one of two dietary treatments based on …


Effect Of Feed Supplementation With Effective Microorganisms (Em) Bokashi Onhepatopancreas And Gut Histology, Growth Performance, And Survival Rate Of Freshwatercrayfish Pontastacus Leptodactylus (Eschscholtz, 1823), Hasan Batuhan Emre Özdoğan, Seval Bahadir Koca, Özlem Özmen, Kami̇l Eki̇nci̇, Emi̇ne Eki̇nci̇, Habi̇l Uğur Koca, Nalan Özgür Yi̇ği̇t Jan 2022

Effect Of Feed Supplementation With Effective Microorganisms (Em) Bokashi Onhepatopancreas And Gut Histology, Growth Performance, And Survival Rate Of Freshwatercrayfish Pontastacus Leptodactylus (Eschscholtz, 1823), Hasan Batuhan Emre Özdoğan, Seval Bahadir Koca, Özlem Özmen, Kami̇l Eki̇nci̇, Emi̇ne Eki̇nci̇, Habi̇l Uğur Koca, Nalan Özgür Yi̇ği̇t

Turkish Journal of Veterinary & Animal Sciences

A sixty-day feeding trial was conducted to investigate the effect of dietary effective microorganisms (EM) Bokashi on organ histology, growth performance, and survival rate of freshwater crayfish Pontastacus leptodactylus (Eschscholtz, 1823). Bokashi serum and powder consisted of two major microorganisms as Bacillus amyloliquefaciens spp. plantarum and Lactobacillus plantarum. Four treatment groups were established in control (without bokashi), bokashi powder 1% and 5% supplemented to diets. Bokashi serum was added at a ratio of 0.01% (v/v) of the total volume of the tank water once a week into which there was a constant flow of fresh water at a flow rate …


Comparison Of Low-Level Laser And Dimethyl Sulfoxide Applications For The Treatment Oftendon Injury In Rabbits, Kardelen Ceydeli̇oğlu, Gülteki̇n Atalan, Duygu Yaman Gram, Gökçen Perk Jan 2022

Comparison Of Low-Level Laser And Dimethyl Sulfoxide Applications For The Treatment Oftendon Injury In Rabbits, Kardelen Ceydeli̇oğlu, Gülteki̇n Atalan, Duygu Yaman Gram, Gökçen Perk

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate and compare the effects of low-level laser therapy (LLLT) and dimethyl sulfoxide (DMSO) on tendon healing in rabbits. A total of 30 adult healthy New Zealand rabbits were used for this study. The rabbits were randomly allocated into 3 groups containing an equal animal number. The experimental tenotomy of the Achilles tendons was created on longitudinal aspect with 10 mm in length. During the postoperative 21-day of period, rabbits in Group I were exposed to laser beam at a rate of 10.8 J/cm$^{2}$ in 5 cm$^{2}$ area for 4 min. In the …


Comparison Of M, Mm And Lts Estimators In Linear Regression In The Presence Of Outlier, Cem Tirink, Hasan Önder Jan 2022

Comparison Of M, Mm And Lts Estimators In Linear Regression In The Presence Of Outlier, Cem Tirink, Hasan Önder

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to evaluate the performance of different estimators that will be used in regression analysis, which is one of the multivariate statistical methods in the presence of outliers in the data set. Sixth month live weight was estimated with various body measurements for Saanen kids taken from a private farm. In the data set, the use and performance of robust estimators were evaluated because the least squares method did not provide reliable results in the case of outliers. M (for Huber and Tukey bisquare) estimator, MM estimator and LTS estimator were used as robust used …


Determining The Genetic Diversity Of Silkworm Lines In Turkey, Ezgi̇ Odabaş, İbrahi̇m Cemal Jan 2022

Determining The Genetic Diversity Of Silkworm Lines In Turkey, Ezgi̇ Odabaş, İbrahi̇m Cemal

Turkish Journal of Veterinary & Animal Sciences

Studies involving molecular genetic characterization of silkworms are quite limited. Therefore, with this study, pure and hybrid silkworm lines raised in Turkey was characterized for the first time by microsatellite DNA markers. Samples were collected from 7 different silkworm lines, of which the pure lines were obtained from the Provincial Directorate of Agriculture and Forestry in Bursa and the hybrid ones from Silkworm Cocoon Association of Turkey (Kozabirlik). DNA samples were extracted from silkworm eggs. Genotyping was done by combining a total of 16 microsatellite markers in a single multiplex. Two hundred and eighty-one alleles were observed for 16 microsatellite …


Investigating Genetic Diversity Of Indonesian Native Cattle Breeds Using Mitochondrialdna 16s Rrna Gene, Restu Misrianti, Sony Hartono Wijaya, Cece Sumantri, Jakaria Jakaria Jan 2022

Investigating Genetic Diversity Of Indonesian Native Cattle Breeds Using Mitochondrialdna 16s Rrna Gene, Restu Misrianti, Sony Hartono Wijaya, Cece Sumantri, Jakaria Jakaria

Turkish Journal of Veterinary & Animal Sciences

This study aimed to estimate the genetic diversity and phylogenetic relationships of Indonesian native and local cattle breeds using 16S rRNA mitochondrial DNA. The study analyzed 93 DNA samples from six cattle breeds. The polymerase chain reaction was conducted using two primer pairs to sequence the complete 16S rRNA gene. The number of the polymorphic site (S), pairwise nucleotide differences (K), haplotype diversity (Hd), and nucleotide diversity (?) were estimated using DnaSP 6 software. PopART v. 1.7 software was employed for the median-joining network analysis, while a neighbor-joining tree was reconstructed based on the Kimura 2-parameter model using MEGA X …


Forage Turnip (Brassica Rapa) Harvested In Different Phases Of Vegetative Stage Andensiled With The Additives Of Molasses And Barley And The Effects Of Additives On Silagequality, In Vitro Digestibility, And Energy Content, Serhat Yildiz, Suphi̇ Deni̇z, Fatma Özkan, Çağri Kale Jan 2022

Forage Turnip (Brassica Rapa) Harvested In Different Phases Of Vegetative Stage Andensiled With The Additives Of Molasses And Barley And The Effects Of Additives On Silagequality, In Vitro Digestibility, And Energy Content, Serhat Yildiz, Suphi̇ Deni̇z, Fatma Özkan, Çağri Kale

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the silage quality, in vitro digestibility and energy content of forage turnip (Brassica rapa) ensiled with molasses or ground barley. Forage turnip was harvested at three different stages: the beginning, middle and end of the flowering period, and it was ensiled with no additive (control), 5% molasses or 4% ground barley. In the trial, pH, nutrient contents, ammonia nitrogen (NH$_{3}$-N) contents, volatile fatty acid (VFA) contents, Fleig scores, in vitro digestibility and energy content were determined. It was observed that the effect of the vegetative stage on the difference between the groups was significant …


Monensin Induces Apoptosis In The Liver Tissue And Primary Hepatocytes Of Chicks, Muhammet Yasi̇n Tekeli̇, Murat Kanbur Jan 2022

Monensin Induces Apoptosis In The Liver Tissue And Primary Hepatocytes Of Chicks, Muhammet Yasi̇n Tekeli̇, Murat Kanbur

Turkish Journal of Veterinary & Animal Sciences

In this study, monensin (MON) was investigated for its apoptotic and genotoxic effects on chick liver tissue and cytotoxic and apoptotic effects on chick primary hepatocytes isolated from perfused liver tissue. Western blotting and real-time PCR (qPCR) were used to determine the apoptotic effect on primary hepatocytes and liver tissue, and the comet assay was used to determine the genotoxic effect on liver tissue. MON decreased cell viability in primary hepatocytes with increasing concentrations. When administered at concentrations of 1 and 10 μM, MON increased the levels of apoptotic p53, caspase-3, and caspase-9, and downregulated the expression of the antiapoptotic …


Do Horses Learn How To Reach For Feed Depending On The Time Of Day?, Agnieszka Ziemianska, Maciej Trojan, Konrad Bazewicz, Justyna Wojtas, Tomasz Próchniak, Iwona Rozempolska-Rucinska Jan 2022

Do Horses Learn How To Reach For Feed Depending On The Time Of Day?, Agnieszka Ziemianska, Maciej Trojan, Konrad Bazewicz, Justyna Wojtas, Tomasz Próchniak, Iwona Rozempolska-Rucinska

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to assess the ability of horses to learn how to open feeder boxes in a direction associated with the time of day. The study involved 10 horses. The first stage of the study consisted in learning how to complete the task. The second stage involved the test and was followed by a control stage. The results indicated the highest number of failed attempts in the leftward movement test. The mean time required by the horses to complete the task of the leftward opening (10.27 s) was twofold longer than in the rightward direction test …


Activities And Mechanisms Of Oregano, Marjoram And Rosemary Essential Oils Against Malassezia Pachydermatitis Isolates From Canine And Feline Otitis, Stefanie Bressan Waller, Marcia Kutscher Ripoll, Anna Luiza Silva, Emanoele Figueiredo Serra, Tábata Pereira Dias, Vittória Bassi Das Neves, Luciéle Pereira De Melo, Patrícia Lindemann, Otávia De Almeida Martins, Angelita Dos Reis Gomes, Renata Osório De Faria, Mário Carlos Araújo Meireles, João Roberto Braga De Mello, Marlete Brum Cleff Jan 2022

Activities And Mechanisms Of Oregano, Marjoram And Rosemary Essential Oils Against Malassezia Pachydermatitis Isolates From Canine And Feline Otitis, Stefanie Bressan Waller, Marcia Kutscher Ripoll, Anna Luiza Silva, Emanoele Figueiredo Serra, Tábata Pereira Dias, Vittória Bassi Das Neves, Luciéle Pereira De Melo, Patrícia Lindemann, Otávia De Almeida Martins, Angelita Dos Reis Gomes, Renata Osório De Faria, Mário Carlos Araújo Meireles, João Roberto Braga De Mello, Marlete Brum Cleff

Turkish Journal of Veterinary & Animal Sciences

Malassezia pachydermatis is an opportunistic yeast found in the ear canal of small animals; however, the current azole-based therapy applied to it has failed to achieve clinical success due to the antifungal resistance. This issue has encouraged the studies in natural products, such as Origanum vulgare (oregano), Origanum majorana (marjoram) and Rosmarinus officinalis (rosemary) essential oils, although their mechanism of action remains unclear. Malassezia pachydermatis specimens deriving from otitis cases in dogs (n = 22) and cats (n = 2) were subjected to CLSI M27-A3. Sorbitol protection and ergosterol effect were analyzed to investigate their mechanism of action. Fungistatic (MIC) …


The Effect Of Osteosubstitution By Platelet-Rich Autofibrin And Hydroxyapatite Ceramic With Ss-Tricalcium Phosphate On Biochemical Parameters Of Blood In Rabbits, Svitlana Shevchenko, Mykhailo Rublenko Jan 2022

The Effect Of Osteosubstitution By Platelet-Rich Autofibrin And Hydroxyapatite Ceramic With Ss-Tricalcium Phosphate On Biochemical Parameters Of Blood In Rabbits, Svitlana Shevchenko, Mykhailo Rublenko

Turkish Journal of Veterinary & Animal Sciences

The paper presents the results of a rabbit blood biochemical study after the substitution of simulated defects in spongy and compact bone tissue by platelet-rich fibrin and its combination with hydroxyapatite granules with ß-tricalcium phosphate. The aim of the work is to establish the biochemical features of reparative osteogenesis in the time of osteosubstitution by platelet-enriched fibrin and its combination with hydroxyapatite and ß-tricalcium phosphate. Bone defects (d = 3 mm) were modeled in rabbits from control and experimental groups (n = 12) under general anaesthesia. In the control group, a replacement was performed with autologous fibrin enriched with platelets …


The Effect Of Microbial Inoculants And Molasses On Quality And In Vitro Digestibility Of Silages Prepared With Different Proportions Of Ryegrass And Hungarian Vetch, Gökhan Şen, Taşkin Erol, Kami̇l Kara, Mehmet Demi̇rci̇, Mehmet Aki̇f Karsli Jan 2022

The Effect Of Microbial Inoculants And Molasses On Quality And In Vitro Digestibility Of Silages Prepared With Different Proportions Of Ryegrass And Hungarian Vetch, Gökhan Şen, Taşkin Erol, Kami̇l Kara, Mehmet Demi̇rci̇, Mehmet Aki̇f Karsli

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to determine the effect of microbial inoculants and molasses on the quality and in vitro digestibility of the silages ensiled from the cultivation of different proportions of ryegrass-Hungarian vetch mixtures grown in Central Anatolian conditions. For this purpose, 5% molasses and 10 g/ton (1.25 × 10$^{11}$ CFU/g) inoculant were added to silages of ryegrass (Lolium multiflorum L.) containing 20%, 40%, 60%, and 80% Hungarian vetch (Vicia pannonica L.), under laboratory conditions. The prepared silages were opened at the end of 60 days and their physical analysis, chemical contents, fermentation parameters, in vitro digestibilities using …


Effects Of Pueraria Lobata Leaf Powder As A Feed Additive On The Immune System And Growth Performance Of Broiler Chickens, Jianping Gong, Dong Wu, Mengchu Li, Xiaoyu Qiu, Ying Jiang, Jie Zhang, Wenping Huang Jan 2022

Effects Of Pueraria Lobata Leaf Powder As A Feed Additive On The Immune System And Growth Performance Of Broiler Chickens, Jianping Gong, Dong Wu, Mengchu Li, Xiaoyu Qiu, Ying Jiang, Jie Zhang, Wenping Huang

Turkish Journal of Veterinary & Animal Sciences

The present research assessed the effect of different levels of Pueraria lobate leaf (PLL) on growth performance, immunity, antioxidant capacity, and blood index in broiler feed. A total of 384 at 21 days old chicks were assigned to 1 of 4 dietary treatments to examine the benefits of basal diet, basal diet +1% PLL, basal diet +2% PLL and basal diet +3% PLL. The trial was divided into 2 time periods, including starter from days 21-49 and finisher from days 50-70. The addition of 2% and 3% PLL powder to diets increased total protein content, triglycerides, albumin, and low-density lipoprotein …


Crude Glycerin And Waste Sesame Seed In The Diets Of Growing Lambs: Impacts On Growth Performance, Nutrient Digestibility, Ruminal Fermentation, Carcass Characteristics, And Meat Fatty Acid Profile, Bora Bölükbaş, İsmai̇l Kaya Jan 2022

Crude Glycerin And Waste Sesame Seed In The Diets Of Growing Lambs: Impacts On Growth Performance, Nutrient Digestibility, Ruminal Fermentation, Carcass Characteristics, And Meat Fatty Acid Profile, Bora Bölükbaş, İsmai̇l Kaya

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to investigate the effects of a combination of crude glycerin and waste sesame seed on the growth performance, rumen fermentation, carcass characteristics, and meat fatty acid profile of lambs. Twenty-four male Karayaka lambs with initial body weights of 26.5 ± 0.53 kg were randomly divided into four groups and raised in individual pens. For 70 days, lambs were fed ad libitum the one of four concentrates: concentrate without CG and WSS (control), concentrate with 10% CG (G), concentrate with 10% WSS (W), and concentrate with 5% CG and 5% WSS (GW). All diets …


High Sensitivity Cardiac Troponin T (Hs-Ctnt) And I (Hs-Ctni) Levels In Dogs With Dirofilaria Immitis, Cemaletti̇n Ayvazoğlu, Şemi̇stan Kiziltepe, Ümi̇t Yaşar, Zehra Gül Yaşar, Pinar Demi̇r, Abuzer Acar Jan 2022

High Sensitivity Cardiac Troponin T (Hs-Ctnt) And I (Hs-Ctni) Levels In Dogs With Dirofilaria Immitis, Cemaletti̇n Ayvazoğlu, Şemi̇stan Kiziltepe, Ümi̇t Yaşar, Zehra Gül Yaşar, Pinar Demi̇r, Abuzer Acar

Turkish Journal of Veterinary & Animal Sciences

Dirofilaria immitis (D. immitis); is a parasitic zoonosis with a prevalence of 53.8%, whose intermediate host is mosquitoes (Anopheles, Aedes, Culex, Taeniorhychus, Mansonia and Armigenes), final hosts are carnivores (dog, cat, fox, etc.) and humans. The causative agent settles in the pulmonary arteries and causes pulmonary hypertension, which can progress to congestive heart failure. Cardiac troponins (cTn), which have recently been used in veterinary cardiology, are considered the gold standard because of their high sensitivity and specificity for myocardial injury. In the study, high-sensitivity cardiac troponin T (hs-cTnT) and high-sensitivity cardiac troponin I (hs-cTnI) levels in healthy …


Effects Of Replacing The Protein Content Of Azolla Pinnata With Concentrate On Physiological And Blood Profiles Changes In Sahiwal Calves, Ninad Bhatt, Ramesh Chandra, Kotresh Prasad Chikkagoudara, Diksha Kandpal, Deepesh Bharat Misra, Nitin Tyagi Jan 2022

Effects Of Replacing The Protein Content Of Azolla Pinnata With Concentrate On Physiological And Blood Profiles Changes In Sahiwal Calves, Ninad Bhatt, Ramesh Chandra, Kotresh Prasad Chikkagoudara, Diksha Kandpal, Deepesh Bharat Misra, Nitin Tyagi

Turkish Journal of Veterinary & Animal Sciences

Eighteen female Sahiwal calves of average four month of age and 54 kg body weight were selected for the study. The animals were divided into three groups (T0, T1, and T2) on body weight basis. The animals were fed as per ICAR feeding standard (2013) for calves for 90 days. The animals in T0 group T0 were fed as per feeding standard while in T1 and T2 groups, fresh Azolla pinnata was fed by replacing 15% and 30% protein content of concentrate with Azolla pinnata on DM basis. The nonsignificant results were obtained for all the physiological parameters. In addition, …


Determination Of Acrylamide Using The Immuno-Enzymatic Method In Commercial Dog And Cat Foods, Ni̇met Kiliç, Bi̇lal Cem Li̇man Jan 2022

Determination Of Acrylamide Using The Immuno-Enzymatic Method In Commercial Dog And Cat Foods, Ni̇met Kiliç, Bi̇lal Cem Li̇man

Turkish Journal of Veterinary & Animal Sciences

Pet animals raised by humans do not have the chance to choose their own food. Therefore, mistakes made by humans in feeding dogs and cats will adversely affect the health of the animal. There are applications at high temperatures at various stages during the production of dog and cat foods. It is predicted that acrylamide (AA) can be formed as a result of a possible Maillard reaction since the cat and dog foods contain starch in its structure. The aim of this study is to determine the AA residue immuno-enzymatically in dry cat and dog feeds from different companies available …


Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil Jan 2022

Investigation Of Antimicrobial Resistance Of Esßl, Amp-C, And Carbapenemaseproducing E. Coli Strains In Retail Poultry Meats, Gülçi̇n Güven, Si̇bel Kizil

Turkish Journal of Veterinary & Animal Sciences

Antimicrobial agents are used for suppressing some bacterial infections during the rearing of poultry. In this study; the antibiotic resistance properties of Extended Spectrum ßeta-lactamase (ESßL), Amp-C, carbapenemase-producing Escherichia coli (E. coli) strains isolated from chicken breast meat were determined by disc diffusion and E-test methods. Additionally, it is aimed to determine antibiotic resistance genes by classical polymerase chain reaction (PCR). In this context, E. coli strains were isolated from 7 of 100 chicken breast meat (fillets) and identified by a rapid identification system (BBL Crystal). Disc diffusion test results of the isolates were evaluated and 100% to tetracycline; ampicillin, …


Pathological And Immunohistochemical Changes In The Liver Of Monocrotaline-Treated Rats, Şevket Soylu, Aydin Çevi̇k, Necati̇ Ti̇murkaan Jan 2022

Pathological And Immunohistochemical Changes In The Liver Of Monocrotaline-Treated Rats, Şevket Soylu, Aydin Çevi̇k, Necati̇ Ti̇murkaan

Turkish Journal of Veterinary & Animal Sciences

Pathological and immunohistochemical changes in livers were investigated in monocrotaline-treated rats. Eighty adult-male Wistar rats were distributed in 5 groups, each containing 16 animals according to monocrotaline dose (Group I: 50 mg/kg, Group II: 100 mg/kg, Group III: 150 mg/kg, and Group IV: 200 mg/kg). The control group was administered with physiological saline. Half of the animals in each group were euthanized at the 18th hour while the remaining half at the 6th week postinoculation. At the end of the 18th hour, the most prominent lesion was hepatocytic apoptosis which was dose-dependent. Apoptotic cells were mostly located in periportal and …


Cryopreservation Of Nili-Ravi Buffalo Bull Semen: Yearly Postthaw Analyses To Depict Climatic Effects, Ejaz Ahmad, Muhammad Shakeel, Zahid Naseer, Adeel Ahmed, Mudussar Nawaz, Muhammad Saleem Akhtar Jan 2022

Cryopreservation Of Nili-Ravi Buffalo Bull Semen: Yearly Postthaw Analyses To Depict Climatic Effects, Ejaz Ahmad, Muhammad Shakeel, Zahid Naseer, Adeel Ahmed, Mudussar Nawaz, Muhammad Saleem Akhtar

Turkish Journal of Veterinary & Animal Sciences

The present study was aimed to observe the postthaw quality of Nili-Ravi buffalo bull semen cryopreserved during heat stress (hot summer months) and normal climatic conditions (fall, winter, and spring months). For this purpose, four (n = 4) adult regular donor Nili-Ravi buffalo bulls were selected to collect and cryopreserve their semen throughout the period of an entire year. The cryopreservation of semen was done following standard freezing protocol. The semen doses cryopreserved during heat stress and normal climatic condition were thawed to determine the sperm motility, motion kinetics, viability, plasma membrane integrity, acrosomal integrity, and morphological abnormalities (head, mid-piece, …


Interaction Effects Of Age And Body Weight Losses During Moulting On The Performance Ofcommercial Laying Hens, Mian Mubashar Saleem, Jibran Hussain, Usman Elahi, Ehsaan Ullah Khan, Muhammad Usman, Syed Ghulam Mohayudin Hashmi, Sohail Ahmad Jan 2022

Interaction Effects Of Age And Body Weight Losses During Moulting On The Performance Ofcommercial Laying Hens, Mian Mubashar Saleem, Jibran Hussain, Usman Elahi, Ehsaan Ullah Khan, Muhammad Usman, Syed Ghulam Mohayudin Hashmi, Sohail Ahmad

Turkish Journal of Veterinary & Animal Sciences

This study evaluated the interaction effects of different age groups and body weight losses during moulting on the productive performance, egg quality, and immune response of commercial laying hens. In total 324 Lohmann Selected Leghorn Lite laying hens of 80 and 108 weeks old were studied. A completely randomized experimental design in factorial arrangement, with 6 treatments of 6 replicates with 9 hens each, was applied. Treatments consisted of 2 production cycles (80 and 108 weeks) and three body weight losses during moulting (20%, 25%, and 30%). Productive performance (body weight, feed intake, egg weight, production percent, egg mass, feed …


Molecular Characterization Of Bacillus Anthracis Isolates Recovered From Nomic Andnonnomic Hosts, Doğan Akça, Fati̇h Büyük, Emre Karakaya, Mustafa Reha Coşkun, Eli̇f Çeli̇k, Mi̇tat Şahi̇n, Özgür Çelebi̇, Sali̇h Otlu, Ali̇ye Gülmez Sağlam, Eray Büyük, Seda Durhan, İzzet Burçi̇n Saticioğlu, Seçi̇l Abay, Tuba Kayman, Fuat Aydin Jan 2022

Molecular Characterization Of Bacillus Anthracis Isolates Recovered From Nomic Andnonnomic Hosts, Doğan Akça, Fati̇h Büyük, Emre Karakaya, Mustafa Reha Coşkun, Eli̇f Çeli̇k, Mi̇tat Şahi̇n, Özgür Çelebi̇, Sali̇h Otlu, Ali̇ye Gülmez Sağlam, Eray Büyük, Seda Durhan, İzzet Burçi̇n Saticioğlu, Seçi̇l Abay, Tuba Kayman, Fuat Aydin

Turkish Journal of Veterinary & Animal Sciences

Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma, soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. …


Determination Of Plant-Specific Retrotransposons In Chicken, Levent Mercan, Ci̇hat Erdem Bülbül, Fati̇h Bi̇lgi̇, Sevgi̇ Marakli Jan 2022

Determination Of Plant-Specific Retrotransposons In Chicken, Levent Mercan, Ci̇hat Erdem Bülbül, Fati̇h Bi̇lgi̇, Sevgi̇ Marakli

Turkish Journal of Veterinary & Animal Sciences

Mobile genetic elements are also known as transposons, comprising a significant proportion of plant and animal genomes. Retrotransposons, a class of transposons, move copy and paste mechanism and, therefore, cause genome obesity. Moreover, the transferability of retrotransposons within and between different kingdoms has provided valuable information for evolutionary relationships in addition to detailed knowledge about genomes. We identified barley-specific retrotransposons (Nikita and Sukkula) and determined polymorphism ratios of these retrotransposons in a chicken genome for the first time to gain new insights for retrotransposons found in different genomes. Transposon sequences belonging to chicken and barley genomes were also investigated to …


Epidemiological Investigation Of Bovine Tuberculosis Infection Dynamics In Turkey, Şahi̇n Çakir, Murat Yildirim, Kadi̇r Serdar Di̇ker, Fevzi̇ye İpek Keski̇n, Selcen Yüksel, Burak Deveci̇, Erhan Akçay Jan 2022

Epidemiological Investigation Of Bovine Tuberculosis Infection Dynamics In Turkey, Şahi̇n Çakir, Murat Yildirim, Kadi̇r Serdar Di̇ker, Fevzi̇ye İpek Keski̇n, Selcen Yüksel, Burak Deveci̇, Erhan Akçay

Turkish Journal of Veterinary & Animal Sciences

A national epidemiological research project was carried out to define the dynamics affecting the epidemiology of bovine tuberculosis (bTB) infection in Turkey and to identify the risk factors. Official veterinarian (OV) and breeder original questionnaires were produced separately as part of this study to collect thorough data regarding the disease from the field. The number of questionnaires that needed to be filled out was decided by 95% confidence interval (CI) and 5% margin of error. The findings of 371 OV and 317 breeder questionnaires completed online across the country were analyzed. In addition, 28 outbreaks determined by random method were …


Estimation Of The Factors Affecting Lactation Milk Yield Of Holstein Cattle By The Adaptiveneuro-Fuzzy Inference System, Aycan Mutlu Yağanoğlu Jan 2022

Estimation Of The Factors Affecting Lactation Milk Yield Of Holstein Cattle By The Adaptiveneuro-Fuzzy Inference System, Aycan Mutlu Yağanoğlu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study is to estimate lactation milk yield of Holstein cattle using first calving age, lactation period, and service period with (ANFIS) adaptive neuro-fuzzy inference system. The input variables for the system in the study were first calving age, lactation period, and service period. The output variable from the system was lactation milk yield. Predicted values obtained from the ANFIS were compared with the observed values. Twenty-seven rule functions are used to obtain the best model and 1000 epochs are used to estimate the accuracy of the training and testing error. The relations between the output and …


The Effects Of Rations Containing Ad Libitum Limestone Grit With Whole Flaxseed On Quails?Performance, Egg Quality, And Blood Parameters, Kadi̇r Emre Buğdayci, Fatma Karakaş Oğuz, Mustafa Numan Oğuz, Cafer Mercan Jan 2022

The Effects Of Rations Containing Ad Libitum Limestone Grit With Whole Flaxseed On Quails?Performance, Egg Quality, And Blood Parameters, Kadi̇r Emre Buğdayci, Fatma Karakaş Oğuz, Mustafa Numan Oğuz, Cafer Mercan

Turkish Journal of Veterinary & Animal Sciences

The purpose of this research is to determine the effects of consuming feed rations containing ad libitum limestone grit with whole or ground flaxseed (10%) on quails? performance, egg quality (shape index, albumen index, yolk index, and Haugh unit), and some blood parameters (hematocrit, cholesterol, and triglyceride) during a period of 8 weeks. Five weeks old quails (Coturnix coturnix japonica; 140 in total) were divided into control and 4 trial groups. The rations of the first (W) and second (G) trial groups consisted of whole or ground flaxseeds (10%), respectively. The composition of the rations of the third (Wg) and …


Development Of Two Novel Sybr Green-Based Quantitative Pcr Assays For Detection Andquantification Of Mycoplasma Gallisepticum And Mycoplasma Synoviae, Mohsen Bashashati, Fereshteh Sabouri Jan 2022

Development Of Two Novel Sybr Green-Based Quantitative Pcr Assays For Detection Andquantification Of Mycoplasma Gallisepticum And Mycoplasma Synoviae, Mohsen Bashashati, Fereshteh Sabouri

Turkish Journal of Veterinary & Animal Sciences

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are considered the most pathogenic and economically devastating avian mycoplasmas. A definite MG and MS diagnosis depends on three main approaches, including isolation and identification, demonstration of specific antibodies, and detection of genetic materials. A fast and precise diagnosis technique is an indispensable prerequisite to prevent and control avian pathogens more effectively. This study aimed to develop and validate convenient SYBR green-based quantitative PCR (qPCR) assays using genome specific primers to rapidly detect and quantify MG and MS. Two sets of primers were designed based on the highly conserved regions of mgc2 and …