Life Sciences Commons^™

'Good Food’ And Food Sovereignty In The South Texas Borderlands: A Qualitative Investigation Of Alternative Retail Food Outlet Managers And Owners, Kanyanat Lertkhonsan

Theses and Dissertations

This case study describes how Alternative Retail Food Outlets (ARFOs) managers and owners in a county in South Texas near the U.S. – Mexico border I am calling Esperanza County, decide what food to eat and to sell. Data from qualitative interviews reveals that the South Texas socioeconomic – cultural context and informants’ economic constraints shaped two different conceptions of good food. At the same time, informants’ socioeconomic backgrounds and sources of good food reveals that the power to define good food – as organic, local, and seasonal – and those that can access it tend to be the educated …

Influence Of Pretreatments On Physiological Status And Post-Planting Survival Of Thornforest Seedlings In A Restoration Context, Douglas E. Mainhart

Theses and Dissertations

Semi-arid ecosystems across the globe have faced land conversion posing a threat to the important ecosystem services they provide. Revegetation-centered restoration efforts in arid regions have low success due to limited water availability and higher temperatures. Typically, practitioners utilize methods that modify field conditions around seedlings to mitigate these stressors. I evaluated the efficacy of in-nursery treatments to improve plant water status. Three pretreatments were applied: elevated CO2, drought hardening, and an antitranspirant foliar spray. My goal was to understand how these pretreatments would impact survival and growth in the field and what physiological traits they altered. Elevated CO2 and …

Incorporating Native Plants In Insectary Strips To Promote Insect Diversity And Below-Ground Beneficial Microbes In South Texas, Lindsey N. Richards

Theses and Dissertations

Farm edges are generally the most undisturbed areas in a farm with diverse vegetation and can enhance agrobiodiversity and provide crucial food and shelter for wildlife, insects, and soil biota. Planting native wildflowers with the ability to reseed and withstand local climatic conditions for semi-permanent vegetation around farm edges or in between crops has potential to biologically control pests. To test this, we ran a two-year study and installed three different flowering insectary strips on a certified organic vegetable farm in South Texas during the winter season. The treatments were: 1) a commercially sourced 17-species native wildflower seed mix; 2) …

Reference Genome Coverage Based Approach For Whole Shotgun Metagenomic Analysis, Daniel Asare Acheampong

Theses and Dissertations

Advancement in metagenomic sequencing technologies have led to a tremendous improvement in public health through the identification of microbial organisms in their natural environment and the role they play. Developments in second and third generation sequencing technologies have contributed immensely to the increase in prokaryotic and eukaryotic reference genomes. Alignment of metagenomic reads to all these reference genomes in the quest to identify the microbial organism present in the sample could be computationally expensive and time consuming, thus the need for a more quick and accurate taxonomic classifiers that can aid in the fast identification of these organisms. Construction of …

Comparative Metabolites Profiling Of Sesamum Indicum Seeds In Context Of Their Origin, Processing And Biological Activity Using Bioinformatics Tools, Bishoy Fayez Abib

Theses and Dissertations

Sesame (Sesamum indicum), is a significant oilseed crop with great economical and nutritional value, encompassing a plethora of primary and secondary metabolites that exhibit pharmacological and therapeutic merits. The present study, using untargeted comparative metabolomics, aims to authenticate and classify sesame seed accessions from different countries of origin i.e., Egypt, Sudan and Nigeria, based on their metabolite variations and in relation to their biological activity and nutritional value such as mineral content. Also, to further assess the impact of processing on sesame’s nutritional value and biological activity using the industrial production of sesame paste as a case study. To achieve …

Understanding Effect Of Ionic Liquid On Metalloproteins: Laccase And Azurin, Aashka Y. Patel

Theses and Dissertations

Interactions between ionic liquids and biomolecules have been of great interest due to the intrinsic properties of ionic liquids and the flexibility to mix and match cations and anions to create unique ionic liquids. A number of ionic liquid-biomolecule studies have focused on the interactions with proteins, including industrially relevant enzymes. One of these, laccase from Trametes versicolor, is a naturally derived enzyme used in the breakdown of phenolic compounds in a wide variety of industries, especially useful in breakdown of lignocellulosic materials. Here, a combination of experiments and molecular dynamics (MD) simulations were used to investigate the interactions …

The Development Of A Genomic Toolbox For Studying The Evolutionary Genetics Of Reptilian Lungs Using The Chicken Model, Logan Thomas Edvalson

Theses and Dissertations

There is a vast diversity in tetrapod lung branching morphology. Phylogenetically, much of the pulmonary diversity among vertebrates appears to arise from the way epithelial tubes branch or form saccular (cyst) structures. Fgf10 activity has been shown to play a critical role in regulating branch versus cyst morphology. We hypothesize that the species-specific differences in lung morphology may be primarily due to species-specific differences in Fgf10 expression. To test this hypothesis, we have performed bioinformatic analyses on the Fgf10 locus and have identified a conserved 11 kb noncoding region that potentially contains the Fgf10 lung enhancer. We are taking a …

An In Silico Study Of The Delta Opioid Receptor Using Small Molecules, Emily Dean

Theses and Dissertations

The DOR is the least studied out of the three opioid receptors (Mu, Kappa, and Delta). The most is known of the Mu Opioid receptor (MOR) and the drugs that target it have led to the global opioid epidemic due to their adverse effects of tolerance and addiction. The DOR is not known for the same adverse effects and therefore, is a promising pharmacological target for the development of new opioid ligands. In this thesis, molecular modeling, simulations and other computational methods are introduced in Chapter 1 where these methods are used to study the activation mechanism of DOR (Chapter …

Coastal Stormwater Pond Pollutants And The Potential For Development Of Antimicrobial Resistance In Vibrio And Enterococcus Bacteria, Cassandra L. Horton

Theses and Dissertations

The Southeastern coastal plain is the most rapidly urbanizing region in the United States. Associated landscape changes which increase imperviousness lead to hydrological cycle alterations, increasing runoff of nonpoint source (NPS) pollution. Urban and agricultural NPS runoff is generally discharged into stormwater ponds, which sequester chemical contaminants, nutrients, and microbes to reduce loading into coastal ecosystems. Studies of these ponds have indicated elevated levels of trace metals, antimicrobial compounds, and bacterial contamination (SCSGC 2018).

Interactions of aquatic pathogens Vibrio vulnificus and Enterococcus faecium with trace metals (arsenic, copper, zinc) and clinically relevant antimicrobials (triclosan, ciprofloxacin, oxytetracycline) commonly found in coastal …

Natural Variation Of Fructose-1,6-Bisphosphatase In Colias Butterflies, Andrea Blair Stokes

Theses and Dissertations

In a process known as the evolutionary recursion, it is possible to follow how adaptation affects natural selection at successive stages of a species’ life cycle. Through studying allele and genotype frequencies of enzymes in glycolysis, a central metabolic pathway, it is possible to document how polymorphisms can shift the evolving functionality of enzymes within or among species. Fructose-1,6-bisphosphatase (FBPase) is an enzyme in gluconeogenesis that converts fructose-1,6-bisphosphate to fructose 6- phosphate. It is also known to convert the unused amino acid carbon skeletons of glycolysis into glycogen storage. Appearing to play a vital role in the acquisition, storage, and …

Monitoring Titanium Dioxide Engineered Particles In Rural And Urban Surface Waters, Md Mahmudun Nabi

Theses and Dissertations

Quantifying and characterizing engineered particles in environmental systems is key for assessing their risk but remains challenging and requires the distinction between natural and engineered particles. The Ph.D. dissertation research described in this thesis contributes to the current attempts to improve environmental nanoparticle analysis and better understand nanoparticle behavior in natural systems, with the focus being on natural streams, and the application of single particle inductively coupled plasma-time of flight-mass spectrometry (SP-ICP-TOF-MS) for distinguishing and tracing the origins of environmental nanoparticles. The objective of this dissertation is to characterize and quantify the concentrations of titanium dioxide engineered particles in urban …

Xenobiotic Exposures On Women’S Reproductive Health, Maria Zubizarreta Mcclam

Theses and Dissertations

Environmental and chemical exposures are continually introduced into our bodies. The female reproductive cycle is complex and particularly sensitive to toxic substances. A recent rise in infertility and reproductive diseases and cancers make reproductive toxicity a public health concern. The overall purpose of this dissertation is to explore how environmental and chemical exposures impact women’s reproductive health and overall wellbeing. To do this, a variety of topics related to women’s reproductive health are explored, including how environmental exposures can impact reproduction, methods for studying reproductive dysfunctions, fertility preservation among cancer patients, and the overall wellbeing of female cancer patients.

The …

Uncovering The Diverse Roles Of The Human Cst (Ctc1-Stn1-Ten1) Complex In Resolving Genomic Stress, Percy Logan Schuck

Theses and Dissertations

The CST (CTC1-STN1-TEN1) complex is a heterotrimeric single-stranded DNA binding protein known to stimulate polymerase α-primase (Pol α) and promote telomere length regulation, genome-wide DNA replication and double strand break repair. However, much of its cellular function remains unknown. The focus of my dissertation work was to uncover novel roles of CST in DNA stress response pathways. In particular, my work relates to understanding the function of CST in sister chromatid cohesion (SCC) and base excision repair (BER) as well as characterizing CST protein interactions. SCC is established following DNA replication by loading of the cohesion complex, which encircles replicated …

Analysis Of The Cytopathogenic Effect Of Different Hiv-1 Vpr Isoforms On Primary Human Cd4+ T Cells And A Model Cell Line, Jonatan Josue Fierro Nieves

Theses and Dissertations

Human Immunodeficiency Virus (HIV) is the causal agent of acquired immunodeficiency syndrome (AIDS), a disease characterized by the depletion of CD4+ T cells which impairs immune response. Analysis of HIV-1 infected patients has identified two distinctive phenotypes that differ in length of time towards the development of AIDS, Rapid Progressor (RP) and Long-Term Non-Progressor (LTNP) patients. The cause of the differences between these two groups is a process that is still under investigation. Hints about a possible cause have been attributed to the discovery of mutations in the viral protein R (Vpr) that have been associated with these phenotypes: mutations …

Finding Biomarkers From Long-Non-Coding Rnas In Serum Of Patients With Non-Alcoholic Fatty Liver Disease, Nouran Yonis

Theses and Dissertations

Nonalcoholic fatty liver disease (NAFLD) is becoming a serious global public health problem as it is a highly prevalent condition, that begins as simple liver steatosis and develops into an inflammatory steatosis form known as non-alcoholic steatohepatitis (NASH). NAFLD has been linked with multiple comorbidities, which in certain cases predisposes to mortality secondary to extrahepatic neoplasms and coronary diseases. On the other hand, NASH leaves patients at risk of developing hepatocellular carcinoma (HCC). The incidence of NAFLD- based HCC is steadily increasing and is projected to surpass the incidence of HCC from viral origins. Long non-coding RNAs (lncRNAs) have been …

Intrinsic Disorder In Proteins Associated With Neurodegenerative Diseases, Akshatha Ganne

Theses and Dissertations

A protein’s structure is determined by its amino acid sequence and post-translational modifications, and provides the basis for its physiological functions. Roughly a third of the proteome in mammalian species comprises proteins that contain highly unstructured or intrinsically disordered regions. Proteins with extensive unstructured regions are referred to as intrinsically disordered proteins (IDPs). Recent advances in experimental and computational analyses predicted multiple interacting partners for the disordered regions of proteins, implying critical roles in signal transduction and regulation of biological processes. This dissertation has studied the connection between the misfolding of a protein and an insoluble protein using the ability …

Policy Evaluation Of The Role Of Extracurricular Activities On Students' Character Building And Academic Excellence: A Case Study Of Cairo's Schools, Nanis Yousry

Theses and Dissertations

The Education policies have usually been given the highest priority globally. This research reflects that the Egyptian educational system currently faces a bottleneck in applying its policies due to significant budgetary constraints. This is seen in public schools, unlike international ones. The research compares and contrasts three educational systems (public, community and international) with the purpose of examining the impact of policy processes on education. This thesis aims to explore how far the existence of extracurricular activities, as a component that complements academic education, is capable of supporting students to acquire the 21st century life skills and fulfill some of …

Nup211 Plays An Important Role In Regulating Mrna Export And Stress Response, Ayana Ikenouchi

Theses and Dissertations

Nup211 is a nuclear pore basket component in Schizosaccharomyces pombe and roles in the gating functions of NPCs. Using RT-qPCR, I found that in nup211-shutoff cells, the transcript levels of genes in mRNA export and stress-response pathways were significantly changed, suggesting nup211 is involved in regulating stress response pathways.

An Exploration Of Factors That Impact Uptake Of Human Papillomavirus Vaccines, David Samuel Redd

Theses and Dissertations

Introduction The discovery and continued development of vaccines is arguably one of the most important innovations in human history. Vaccination greatly reduces the worldwide incidence and transmission of diseases, preventing permanent injury and premature death. Mass vaccination campaigns have led to the eradication or partial eradication of severe infectious such as smallpox and polio, have reduced childhood mortality, and has led to an overall increase in average health in the human population globally. Despite the documented benefits of vaccination, vaccine hesitancy is increasing, and the uptake of some vaccines is low. Vaccines have been so successful at preventing disease that …

Big Data Meta-Analyses Of Transcriptional Responses Of Human Samples To Orthohantavirus Infection And Shotgun Metagenomics From Crohn's Disease Patients., John L. Krapohl

Theses and Dissertations

Hantavirus is a dangerous zoonotic viral pathogen that is found across Asia, Europe, and the Americas. This virus causes a range of symptoms from flu-like malaise to heart failure and death. It is normally transmitted to humans via the aerosolized feces or urine of infected rodents. Currently, there are no known treatments for the disease, and it continues to threaten human health in endemic areas. In order to identify possible future therapeutic targets, we ran a meta-analysis of existing transcriptomic data collected from infected human tissue. Several genes and cellular pathways were identified, in addition to several potential therapeutics that …

Regulation Of T Cell Activation By The Cd5 Co-Receptor And Altered Peptides, Characterization Of Thymidine Kinase-Specific Antibodies, And Integrating Genomics Education In Society, Kiara Vaden Whitley

Theses and Dissertations

Helper T cells (Th) are a vital component of the immune system responsible for directing other immune cells to eliminate pathogens and cancer. Specifically, Th cells facilitate B cell and cytotoxic T cell (Tc) activation and recruitment and enhance their function against cancer and infectious diseases. Th cells are a valuable resource for improving Tc responses in cancer treatment and have become a focus of immunotherapeutic research. While it is increasingly clear that helper T cells serve an important role, the details about which entities produce an effective Th cell response remain unclear. CD5 is a T cell co-receptor that …

Effects Of Localized Irrigation And Fertilizer On Woody Plant Establishment In Degraded Semi-Arid Environments, Holley M. Lund

Theses and Dissertations

Semi-arid native plant communities worldwide are often disturbed either intentionally or incidentally by human activity. In order to restore ecological function after human activities cease, native plant communities need to be restored. Woody plants are important to ecological function for many reasons including reducing erosion and providing food and shelter for wildlife. Unfortunately, woody plant establishment in these areas has proven to be challenging. Direct seeding efforts can be hindered by poor germination and low seedling emergence. To overcome this, seedling transplants are often used in harsh sites. However, transplanted woody seedlings often experience high mortality during the first year, …

Development Of A Rhizobium Seed Coating To Establish Lupine Species On Reclaimed Minelands, Bridget May Calder

Theses and Dissertations

Symbiotic interactions among various organisms are often necessary for one or both individual's survival. These symbiotic relationships must be considered in restoration projects to allow for the successful establishment of the species. Rhizobia are nitrogen-fixing bacteria found in symbiotic relations with legumes. By utilizing this relationship, restoration practitioners can establish native legume species more successfully while repopulating soil microorganisms into degraded soils. Despite the potential benefits a rhizobium inoculant can have on restoration efforts, minimal research has been done to understand the impacts this treatment has on specific species and the systems they are employed within. Our research goal was …

Effect Of Cover Crops, Grazing And Tillage Practices On Soil Microbial Community Composition, Function, And Soil Health In East Central Mississippi Soybean Production System., Namita Sinha

Theses and Dissertations

Integrating crop and livestock is being considered to improve soil health by carbon sequestration. A two-year study (2019-2021) at CPBES in Newton, MS was aimed to evaluate soil microbial diversity in the warm, humid regions, specifically southeastern USA. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and microbial diversity characterization were performed using QIIME2^®. Soil fungal diversity showed significant differences (alpha diversity, p = 0.031 in yr. 2020 and beta diversity, p = 0.037 in yr. 2021). Canonical Correspondence Analysis (CCA) and Mantel test showed significant influence on fungal diversity due to …

Phenotypic Evaluation Of Energycane (Saccharum Spp.) Genotypes In Northcentral Mississippi, Wyatt Armistead Eason

Theses and Dissertations

As fossil fuel supplies decrease and concerns of climate change increase, the search for alternative sources of fuel has pushed biomass crops to the forefront of discussion. Saccharum spontaneum readily hybridizes with commercial sugarcane and lends cold tolerance and greater yields to the hybrid progeny, called energycane. Twenty genotypes were tested against an energycane variety (Ho 02-113) as a control. Two locations were tested: the HH Leveck Animal Research Center (planted in 2019), and the Bearden Dairy Research Center (planted in 2020). The Bearden Dairy Research Center yielded significantly higher than the HH Leveck Animal Research Center regarding dry matter …

Classification Models For 2,4-D Formulations In Damaged Enlist Crops Through The Application Of Ftir Spectroscopy And Machine Learning Algorithms, Benjamin Blackburn

Theses and Dissertations

With new 2,4-Dichlorophenoxyacetic acid (2,4-D) tolerant crops, increases in off-target movement events are expected. New formulations may mitigate these events, but standard lab techniques are ineffective in identifying these 2,4-D formulations. Using Fourier-transform infrared spectroscopy and machine learning algorithms, research was conducted to classify 2,4-D formulations in treated herbicide-tolerant soybeans and cotton and observe the influence of leaf treatment status and collection timing on classification accuracy. Pooled Classification models using k-nearest neighbor classified 2,4-D formulations with over 65% accuracy in cotton and soybean. Tissue collected 14 DAT and 21 DAT for cotton and soybean respectively produced higher accuracies than the …

Incorporating Follicle Stimulating Hormone To Stimulate Multiple Corpora Lutea For Embryo Transfer In Beef Cattle, Bryan Carter

Theses and Dissertations

Assisted reproductive technologies, such as estrus synchronization and embryo transfer, can aid producers in improving genetics by increasing the number of progeny produced from elite females. The success of embryo transfer is dependent on a viable, competent embryo and a recipient with a receptive uterine environment. Follicular development and luteinization are pertinent for the recipient to establish a functional corpus luteum (CL) that can produce adequate concentrations of progesterone (P4) and provide a uterine environment conducive for the establishment of pregnancy. The objective of this study was to determine if exogenous follicle stimulating hormone (FSH), would increase the number of …

A Biomechanical Approach To Prevent Falls In Ergonomic Settings, Sachini Kodithuwakku Arachchige

Theses and Dissertations

Introduction: Fall-related injuries are exceptionally prevalent in occupational settings. While endangering the workers’ health, falls cause poor productivity and increased economic burden in the workplace. Hence, identifying these threats and training workers to achieve proper postural control is crucial. Purpose: Study 1: To investigate the ankle joint kinematics in unexpected and expected trip responses during single-tasking (ST), dual-tasking (DT), and triple-tasking (TT), before and after a physically fatiguing exercise. Study 2: To investigate the impact of virtual heights, DT, and training on static postural stability and cognitive processing. Methods: Study 1: Twenty collegiate volunteers (10 males and females, one left …

The Effects Of Moderate And High Temperatures On Growth, Metabolism, And Blood-Oxygen Affinity In Ictalurus Spp., Brandon Gerhart

Theses and Dissertations

Despite the growing use of hybrid catfish (Ictalurus furcatus × I. punctatus) in aquaculture, few studies have investigated the comparative effects of temperature on physiological performance with other cultured catfish. Therefore, growth, metabolism, and blood-oxygen affinity of blue (I. furcatus), channel (I. punctatus), and hybrid catfish were compared at moderate (23°C) and high (33°C) water temperatures. Channel catfish had the greatest growth and were in better condition compared to blue and hybrid catfish. Hybrid catfish standard metabolic rates did not differ from blue or channel catfish, while hybrid catfish had higher maximum metabolic rates, …

Nondestructive Assessment Of Flexural And Tensile Properties For Southern Pine Structural Lumber, Marly Gabriela Carmona Uzcategui

Theses and Dissertations

The flexural and tensile properties of visually graded southern yellow pine lumber were modeled. Longitudinal and transverse vibration techniques and proof-loading bending tests were used to assess the flexural and tensile properties of southern pine lumber. The properties evaluated were dynamic modulus of elasticity (dMOE), static modulus of elasticity (E_b), tension modulus of elasticity (E_t), and ultimate tensile stress (UTS). The tensile properties were evaluated in the direction parallel to the grain. This study presents the results of tests conducted on No. 2 2 × 6 and 2 × 10 southern pine lumber of two different …