Life Sciences Commons^™

Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden

Theses and Dissertations

The nursery/greenhouse industry is innovative. There have been several reports in the popular literature of non-traditional adjuvants used in plant propagation; however, these claims have never been evaluated in a scientific setting. Five studies, conducted at the South Mississippi Branch Experiment Station in Poplarville in 2019 and 2021 determined the impact that adding honey to water-soluble auxin solutions and surfactants to foliar applied auxin solutions on physiological responses associated with adventitious root formation. Treatments in studies presented in chapters two and three included three honey sources (multiflora, local, and Manuka). Honey-infused water-soluble auxin solutions affected plant species but not in …

Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers

Theses and Dissertations

A long-held practice is to mix agricultural soil with a soil amendment to improve growing conditions in crops. A common soil amendment is biosolids produced from both municipal and dairy wastewater due to the macro- and micronutrients within it. Both the agricultural and wastewater industries are participating in the Circular Economy concept (CEC). Two experiments explored using expended bioelectrochemical systems (BES) that treated either synthetic dairy wastewater (DWW) or synthetic municipal wastewater (SWW) as soil amendments to improve corn plant growth when treated with three different nutrient treatments: 100%- 50%- and 0% Hoagland Nutrient Solutions. Biochar and used terracotta clay …

Assessing The Legacy Of Erosion And Flood Control Management Efforts On The Fish Assemblages And Physical Conditions Of Yazoo Basin Bluff Hill Streams, Nicky M. Faucheux

Theses and Dissertations

The hills of Yazoo Basin have a long history of land use modification and subsequent erosion and flood control issues. In response, federal actions were taken to address these issues beginning after the Mississippi River flood of 1927. Four major flood control reservoirs were built in 1932-1957, and instream low-drop grade control structures (GCS) were installed beginning in the 1980s. The objective of my dissertation was to ascertain the long-term effects of these efforts on stream fish assemblages and channel morphology. To assess whether the reservoirs affected upstream fish assemblages as barriers to recolonization by fluvial fishes or as source …

Effects Of Microcystin-Lr On Channel Catfish (Ictalurus Punctatus) Susceptibility To Microbial Pathogens (Aeromonas Hydrophila And Edwardsiella Piscicida), Alison Marchant

Theses and Dissertations

Microcystin-LR is a hepatotoxin produced by cyanobacteria. Aeromonas hydrophila and Edwardsiella piscicida infections are leading causes of losses in market-sized channel catfish (Ictalurus punctatus). These older fish should have natural immunity in place and a predisposing factor is likely a prerequisite for these disease outbreaks. While microcystin-LR rarely causes mortality in warm-water aquaculture, we believe it may be a predisposing factor that leads to bacterial disease outbreaks during the summer months due to its ability to damage the liver. Our study investigated microcystin-LR’s effects on channel catfish susceptibility to these pathogens. We found that a sublethal dose of microcystin-LR induced …

Reassessing Nitrogen Management For Maize Production In Mississippi, Camden Dawson Oglesby

Theses and Dissertations

12 N treatments in 2020 and 11 in 2021 were replicated four times over four locations in a randomized complete block design. The first research tested the efficacy of CYG for Mississippi corn (Zea mays L.) production. The optimum N rates were calculated by fitting four models. Differences between the CYG rate and AONR were compared. AONR varied from 134 to 301 kg N ha–1 at different management levels. When we compared the AONR to the CYG rate, the CYG rate over-recommended N in 12 of the 14 possible comparisons. The second study compared different VIs, methods, and sensors at …

Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg

Theses and Dissertations

The work here presents two main parts. In the first part, chapters 1 – 3 focus on dynamical systems modeling in plant immunity, whereas chapters 4 – 6 describe contributions to computational modeling and analysis of proteomics and genomics data. Chapter 1 investigates dynamical and biochemical patterns of reversibly oxidized cysteines (RevOxCys) during effector-triggered immunity (ETI) in Arabidopsis, examines the regulatory patterns associated with Arabidopsis thimet oligopeptidase 1 and 2’s (TOP1 and TOP2), roles in the RevOxCys events during ETI, and analyzes the redox phenotype of the top1top2 mutant. The second chapter investigates the peptidome dynamics during ETI …

Investigation Of The Material Properties Of Poly Pipe Irrigation Tubing For Identifying Performance Characteristics By Thickness, Victor Frank Carey Iv

Theses and Dissertations

Poly Pipe Tubing is widely used in the Mississippi Delta for supplemental irrigation of crops during the growing season. Computerized Hole Selection (CHS) is a tool used to create a prescriptive hole punching map to manage flow and minimize tail-water runoff. Some producers have documented that CHS does not work on their farm. The common thought of failure in poly pipe tubing is the rupturing or splitting of the tubing. This research was based on the thought that failure occurs before the tubing is ruptured and is caused by over pressurization during an irrigation event. Static testing revealed that there …

The Emerging Value Of The Viroid Model In Understanding Plant Responses To Foreign Rnas, Junfei Ma

Theses and Dissertations

RNAs play essential roles in various biological processes. Mounting evidence has demonstrated that RNA subcellular localization and intercellular trafficking govern their functions in coordinating plant growth at the organismal level. Beyond that, plants constantly encounter foreign RNAs (i.e., RNAs from pathogens including viruses and viroids). The subcellular localizations of RNAs are crucial for their function. While numerous types of RNAs (i.e., mRNAs, small RNAs, rRNAs, tRNAs, and long noncoding RNAs) have been found to traffic in a non-cell-autonomous fashion within plants, the underlying regulatory mechanism remains unclear. Viroids are single-stranded circular noncoding RNAs, which entirely rely …

Evaluating The Impacts Of Waterlogging Stress On Cowpea (Vigna Unguiculata L.) Growth Traits And Physiological Performance, Omolayo Joshua Olorunwa

Theses and Dissertations

The progressive increase in the global population and the rapidly changing climate have put unprecedented pressure on crop production. Cowpea is one of the world’s most important leguminous crops, contributing to food security and environmental sustainability. However, cowpea productivity is limited due to waterlogging stress. The main objective of this study was to explore physiological and biochemical mechanisms to understand how cowpea genotypes respond to waterlogging stress. Four studies were conducted in controlled and field conditions to achieve these objectives. Study 1 characterized the waterlogging tolerance of 30 cowpea genotypes in a controlled environment using 24 morphophysiological parameters with waterlogging …

Healthy Eating Index And Body Fat Distribution, Masar Ozrail

Theses and Dissertations

Body fat distribution has been identified as a more significant risk factor for metabolic-related diseases. This study investigated whether body fat distribution affects dietary outcomes (quality and intake), anthropometric measures, body composition, and skin carotenoid levels. A cross-sectional study with 91 female students was conducted. Thirty-one percent of participants (n=28) had an android body fat distribution. Body fat distribution was associated with WC and WHtR (p

The Use Of 87sr/86sr Isotopic Ratios To Assess Natal Areas And Movement Of Juvenile Gulf Sturgeon Acipenser Oxyrinchus Desotoi In The Pascagoula And Pearl River Systems, Joshua Neary

Theses and Dissertations

The effects of water and dietary influence, and time duration for discernment, of pectoral fin spine ⁸⁷Sr/⁸⁶Sr were evaluated in Atlantic Sturgeon Acipenser oxyrinchus oxyrinchus. Fish were subjected to ⁸⁷Sr-enriched water (0.1 mg/L) or diet (0.25 migrogram/g or 0.5 microgram/g) for 1, 2, 3, 6, or 12 weeks. Fish were also held at 15 or 25°C to determine if temperature influenced ⁸⁷Sr/⁸⁶Sr. Results found fin spine ⁸⁷Sr/⁸⁶Sr changed in water after 1 week and diet after 12 weeks suggesting water is the primary influence. In wild Gulf Sturgeon A. o. …

Development Of A Novel Loop-Mediated Isothermal Amplification (Lamp) Assay For The Rapid Detection Of Clostridium Perfringens, Deepa Chaudhary

Theses and Dissertations

Clostridium perfringens (C. perfringens) causes diseases such as necrotic enteritis in poultry. Current methods for early detection of C. perfringens, including culture, and molecular techniques (PCR and qPCR), have logistical limitations in detecting pathogens on-site because they require specialized equipment and long sample preparation time. This study describes developing a highly sensitive on-site LAMP assay combined with a simple DNA extraction method to detect C. perfringens. Results showed that the LAMP assay was highly specific and sensitive with the optimal reaction conditions: 63°C for 30 mins with primer concentration of 1.2 mM FIP/BIP and 0.2 mM F3/B3. The HotSHOT method …

Effects Of Microbial Transglutaminase On Gluten-Free Sourdough Bread Structure And Loaf Characteristics, Anna J. Redd

Theses and Dissertations

In an effort to mimic the continuous protein matrix found in gluten-containing breads, the effects of the enzyme microbial transglutaminase were tested in gluten-free (GF) sourdough breads containing five different GF flours: chickpea, brown rice, white rice, oat, and quinoa. The utilization of transglutaminase in GF sourdough bread applications was shown to improve some of the final bread characteristics of GF breads. White and brown rice GF sourdough loaves at 24 hours showed a 28% and 13% decrease in crumb firmness with the addition of 2 U/g dough, respectively. Quinoa-GF sourdough breads at 24 hours showed a 6% increase in …

Motivation Toward Sport, Physical Activity And Exercise Between College Students In Hungary And The United States During Covid-19, Whitney Gene Davies

Theses and Dissertations

During the COVID-19 pandemic many researchers noted changes in exercise, physical activity, and sports behaviors. The current study attempts to identify aspects of motivation for physical activity, exercise, and sport for students in two countries, the USA and Hungary. The current study used the Sports Motivation Scale II to measure students’ motivation based on the Self-Determination Theory. Results indicated that students in the USA were more motivated for exercise, physical activity, and sport during the COVID-19 pandemic than were their Hungarian counterparts. Students in the United States scored higher (MUS = 17.88) in overall motivation than their Hungarian counterparts (MHungary …

The Use Of Inertial Measurement Unit For The Characterization Of Multiple Functional Movement Patterns In Individuals With Chronic Ankle Instability, Seunguk Han

Theses and Dissertations

Patients with a history of lateral ankle sprain (LAS) may experience different levels of mechanical and/or sensorimotor deficits following their injuries. Although various factors, such as structural damage, sensorimotor adaptation, perceived instability, swelling and/or pain, can develop and perpetuate the condition of chronic ankle instability (CAI), most previous CAI research on biomechanics has considered all patients with CAI as a homogeneous group. Recent research has clustered patients with CAI into six distinct movement patterns during a maximal jump-landing/cutting task. This approach could motivate clinicians to develop appropriate rehabilitation programs for each patient with CAI depending on their movement patterns. However, …

Are Changes In Muscle Blood Flow Associated With The Age-Related Decrease In Critical Power?, Abigail Dorff

Theses and Dissertations

Aging results in lower exercise tolerance, manifested as decreased Critical Power (PCRIT). Aging is also associated with reduced physical activity, decreased muscle mass, and altered muscle blood flow, all of which may contribute to the age-related decrease in PCRIT. Purpose: The purpose of this study was to determine if the age-related decrease in PCRIT occurs independently of changes in physical activity and muscle mass and if it is related to impaired muscle blood flow. Methods: 10 Old (63.1 ± 2.5 years, 5 female and 5 male) and 10 Young (24.4 ± 4.0 years, 5 female and 5 male) physically active …

Sitting Time And Insulin Resistance In 6,931 United States Adults: The Role Of Abdominal Adiposity, Kayla Marie Parker

Theses and Dissertations

This cross-sectional investigation of 6,931 U.S. adults examined the relationship between sitting time and insulin resistance. A primary objective was to evaluate how this relationship was mediated by the following variables: age, sex, race, year of assessment, cigarette smoking, physical activity, body mass index (BMI), and waist circumference. Self-reported sitting time, measured in minutes per day, was the exposure variable. Insulin resistance, indexed by the homeostatic model assessment of insulin resistance (HOMA-IR), was the outcome variable. Data were used from the 2011-2018 National Health and Nutrition Examination Survey (NHANES). Results showed a strong, positive association between sitting time and insulin …

Bobcat Identification, Abundance, And Behavior At Road Mitigation Structures In South Texas, Victoria Hanley

Theses and Dissertations

Highways in South Texas fragment the landscape and result in high road mortality rates for the endangered US ocelot (Leopardus pardalis). In response, the Texas Department of Transportation constructed nine wildlife crossing structures (WCS) on Farm-to-Market (FM) 106 which adjoins Laguna Atascosa National Wildlife Refuge in Cameron County, Texas. With ocelots being rare and elusive, bobcats (Lynx rufus) are often used as a surrogate species to study felid behavior at road mitigation structures in South Texas. Bobcats have unique markings which allow them to be individually identified. Three methods of individual identification of bobcats using camera …

The Role Of Lncrna Uca1 In Colorectal Cancer Progression And Metastasis, Sophia M. Leslie

Theses and Dissertations

Colorectal cancer (CRC) is the primary cause of cancer-related deaths worldwide, and it is the third most common cancer diagnosed in adults in the United States according to the American Cancer Society. We have identified lncRNA UCA1, a poor prognosis marker in CRC, responsible for increased proliferation and upregulating key genes related to glucose metabolism. Recombinant cell lines were generated to overexpress and knockdown UCA1 to establish the phenotype and to investigate the properties endowed in metastasis. A vital aspect of the metastatic progression is when the cell survives detachment from the extracellular matrix. At 36 hours of anchorage-independent stimulation, …

Dynamic Soil Properties In The Lower Rio Grande Valley: Understanding Responses Of Infiltration Rate, Bulk Density, Aggregate Stability To Different Tillage Practices, Temiloluwa Faith Awotoye

Theses and Dissertations

Dynamic Soil Properties (DSPs) are soil properties that change in response to both natural and human-caused interruptions and pressures such as agro-based and wildland management. These soil properties can transform for months or even years in response to the farm use and management procedures. This research project investigates the relationship between different dynamic soil physical properties and tillage practices in the Texas Hidalgo sandy clay loam benchmark soil series. Data analyses was done to compare the effect of tillage to these soil physical properties and build a discussion about relationships between on-farm practices to other aspects of farm viability. We …

The Molecular Evolution Of Insect Opsin Genes, Maria Jose Cardenas Muedano

Theses and Dissertations

Opsins are part of the superfamily of G-coupled receptor proteins and together with chromophores, are responsible for initiating the signal transduction cascade responsible for animal vision. Since opsins have a critical role on visual perception these genes are crucial for a myriad of adaptive traits and behaviors and should show diverse signs of natural selection at the molecular level. Understanding the evolution of opsins across the wide diversity of insect groups will allow us to better understand the evolutionary pressure on insect opsins and serve as a model for both other gene systems and other taxonomic groups of animals that …

An Assessment Of Physical And Microbial Dynamic Properties Sensitive To Tillage Practices In Hidalgo Sandy Clay Loam Soils, Rocio N. Hernandez

Theses and Dissertations

Mechanized tillage is known to impact the dynamic physical, biological and chemical properties of a given soil, and as a result, can cause systematic harm to the soil’s function (Alam et al., 2014). The aim of this study is to assess how a series of land management tillage practices will impact dynamic physical and biological soil health parameters such as aggregation, carbon, respiration, and enzymatic activity (β-glucosidase). According to Acir et al (2020), the aforementioned soil parameters are both co-influenced and dependent, indicating a response to tillage is likely. A total of 198 samples of Hidalgo series soils were collected …

Effects Of Roundup Exposure On Redox Status, Cellular Apoptosis, And Antioxidant And Osmoregulatory Enzyme Expressions In Goldfish (Carassius Auratus), Md Imran Noor

Theses and Dissertations

Intense anthropogenic activities of industrialized nations dramatically increase environmental pollution. This study focused on the effects of Roundup, a glyphosate-based herbicide, exposure (low- and high-dose: 0.5 and 5 mg/L for 2 weeks) on dinitrophenyl protein (DNP), nitrotyrosine protein (NTP), superoxidase dismutase (SOD), catalase (CAT), Na+ /K+ - ATPase (NKA), and renin expressions, and cellular apoptosis in the gills and kidneys of goldfish. Histopathological analysis showed widespread tissue damage in both gills and kidneys. Immunohistochemical analysis provided insights into the expression of molecular biomarkers in tissues. Fish exposed to Roundup exhibited a significant (P<0.05) upregulation in DNP, NTP, SOD, and CAT expressions, and apoptotic nuclei in both tissues. Additionally, exposure to Roundup significantly increased renin expression in kidneys and decreased NKA expression in gills. Overall, our results suggest that exposure to Roundup induces oxidative/nitrative stress and cellular apoptosis and alters osmoregulatory and antioxidant systems which may lead to impaired physiological functions in goldfish.

Foxo Factors Regulate The Expression Of Canonical Wnt Target Genes In A Set Of Basal-Like Breast And Glioblastoma Multiforme Cancer Cell Lines, Shania Pintor

Theses and Dissertations

Basal-like breast cancer (BBC) and Glioblastoma Multiforme (GBM) each express stem gene expression signatures which are partly driven by FOXO transcription factors. Preliminary data from Gene Set Enrichment Analysis (GSEA) with RNA Seq. data indicated that a set of WNT target genes including LEF1, and TCF7 were robustly induced after 48-hours of AS1842856 (FOXO1 inhibitor) treatment. These same genes were also induced in GBM cell lines U87MG, LN18, LN229, A172, and DBTRG upon treatment with AS1842856. In contrast to FOXO1 inhibition, RNAi targeting of FOXO1, led to reduced LEF1, and TCF7 gene expression in BT549 and U87MG …

Influence Of Glucose And Lactic Acid On Macrophage Transition In Pancreatic Tumors, Anyssa A. Rodriguez

Theses and Dissertations

Pancreatic cancer is the seventh leading cause of cancer-related mortality worldwide. According to the American Cancer Society, it is the eleventh most common cancer diagnosed in both men and women in the United States. Pancreatic ductal adenocarcinoma (PDAC) comprises 90% of all pancreatic malignancies and is associated with poor clinical outcome because of late diagnosis and resistance to therapy. In addition, studies show that the tumor microenvironment has become an emerging interest in cancer research due to its complex components and unique characteristics that aid in cancer initiation, maintenance, and progression. Accumulating evidence signifies that tumor associated macrophages (TAMs) play …

Weather Parameters Influencing The Incidence Of Citrus Canker Caused By Aw Strain In The Rio Grande Valley, Amit Sharma

Theses and Dissertations

Citrus canker caused by bacterium Xanthomonas citri subsp. citri (Xcc) seriously affects the citrus industry by making the fruit unmarketable due to unsightly lesions on the fruit. Canker caused by Aw strain of Xcc was reported in the citrus trees located in the residential areas of the Rio Grande Valley (RGV). Canker severity differs amongst cultivars/varieties, and it is influenced by prevailing environmental conditions. Multiple regression modeling of the disease incidence with the environmental variables such as temperature, humidity, windspeed, wind gust, and rainfall was performed to understand the environmental conditions that are favorable for spread of citrus …

Effects Of Disturbance (Mowing) On Florivory And Floral Defenses In Solanum Elaeagnifolium, A Noxious And Worldwide Invasive Weed, Alejandro Rafael Vasquez Marcano

Theses and Dissertations

Chapter 1: Literature Review- In this literature review we have examined weed management as it concerns urban and agricultural ecosystems, as well as the need for mowing assessments as it relates to floral traits and defense against herbivory.

Chapter 2: Experiments- In the experiments we examined the role of mowing on floral growth traits, floral defense traits, and their effect on Manduca sexta specialist herbivores.

Chapter 3: Results- In the results we found SLN flowers to have larger diameters than unmowed flowers; however, flower mass was in line with our predictions as unmowed flowers were heavier in high mowing frequency …

South Texas Wildlife Activity Across A Fragmented Landscape And Road Mitigation Corridor, Caitlin K. Brett

Theses and Dissertations

Wildlife crossing structures (WCS) and roadside fencing are commonly installed to mitigate habitat fragmentation, wildlife road mortalities, and other negative effects that roads can have on the surrounding landscape. Eight such WCS were constructed below Farm-to-Market (FM)106 in Cameron County, Texas, across a 16 km corridor transecting the Laguna Atascosa National Wildlife Refuge. These WCS, paired with adjacent roadside fencing, were intended to prevent road mortalities of the endangered ocelot (Leopardus pardalis) and to mitigate the barrier effect of FM106 on this and other meso-mammal species. This study will analyze camera trap data from roadside and habitat reference …

Grapefruit Peel Biochar Applications For Immobilizing Copper And Lead In Soil, Michael Alfredo Navarro

Theses and Dissertations

This study investigated the efficacy of grapefruit peel (GP) derived biochar materials (two pyrolysis temperatures 450°C and 800°C) as a potential adsorbent to immobilize heavy metal (copper and lead) in a local sandy loam clay soil. A batch adsorption test with heavy metal concentrations at 50 mg L-1, and 100 mg L-1 revealed that soil itself had the highest immobilization percentage compared to the GP biochars. A soil column experiment was conducted to determine how much the GP biochar samples and the tested soil will exhibit adsorptive characteristics for heavy metals when 50 ml of 50 mg L-1 copper or …

Wildlife Road Mortality Patterns In South Texas And Survey Methodology Improvement, Bradley E. Beer

Theses and Dissertations

Mortalities of wildlife caused by collisions with vehicles along roads are increasing in prevalence, threatening the existence of various species and populations. A better understanding of how mortalities change in response to natural and anthropogenic variables and efficient methods of obtaining mortality data are essential to mitigating such mortalities. This thesis investigates several key elements to improving road mortality surveys in south Texas. First, it was found that road mortality survey counts did not change under a pandemic-related lockdown and that 2 mortality survey observers detect more mortalities than 1. Analysis of brown pelican groundings on Texas State Highway 48 …